ACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTGCTACAA

TGGGCAGAACAAAGGGCAGCGAAACCGCGAGGTTAAGCCAATCCCACAA

ATCTGTTCTCAGTTCGGATCGCAGTCTGCAACTCGACTGCGTGAAGCTG

GAATCGCTAGTAATCGCGGATCAGCATGCCGCGGTGAATACGTTCCCGG

GCCTTGTACACACCGCCCGTCACACCACGAGAGTTTGTAACACCCGAAG

TCGGTGAGGTAACCTTTTTGGAGCCAGCCGCCGAAGGTGGGACAGATGA

TTGGG.

TABLE 1

BLAST result of 16S rRNA gene sequencing of strain M-SHDS-22

Pairwise
Mismatch/

Rank
Name
Strain
Accession
Similarity(%)
Total nt

1
Bacillus amyloliquefaciens
DSM 7
FN597644
99.85975
2/1426

2
Bacillus siamensis
KCTC 13613
AJVF01000043
99.85975
2/1426

3
Bacillus velezensis
CR-502
AY603658
99.85369
2/1367

4
Bacillus subtilis
NCIB 3610
ABQL01000001
99.57924
6/1426

5
Bacillus nakamurai
NRRL B-41091
LSAZ01000028
99.57924
6/1426

6
Bacillus cabrialesii
TE3
MK462260
99.50912
7/1426

7
Bacillus inaquosorum
KCTC 13429
AMXN01000021
99.50912
7/1426

8
Bacillus stercoris
JCM 30051
MN536904
99.50912
7/1426

9
Bacillus vallismortis
DV1-F-3
JH600273
99.43899
8/1426

10
Bacillus atrophaeus
JCM 9070
AB021181
99.36886
9/1426

11
Bacillus tequilensis
KCTC 13622
AYTO01000043
99.36886
9/1426

12
Bacillus halotolerans
ATCC 25096
LPVF01000003
99.36886
9/1426

13
Bacillus spizizenii
NRRL B-23049
CP002905
99.36886
9/1426

14
Bacillus mojavensis
RO-H-1
JH600280
99.29874
10/1426

15
Bacillus glycinifermentans
GO-13
LECW01000063
98.52632
21/1425

16
Bacillus paralicheniformis
KJ-16
KY694465
98.45614
22/1425

17
Bacillus licheniformis
ATCC 14580
AE017333
98.24561
25/1425

18
Bacillus haynesii
NRRL B-41327
MRBL01000076
98.17544
26/1425

19
Bacillus sonorensis
NBRC 101234
AYTN01000016
98.10526
27/1425

As shown in Table 1, the strain M-SHIDS-22 belonged to the genus Bacillus sp. The strain M-SHDS-22 was compared with the strain whole genome data of Bacillus amyloliquefaciens (DSM7) model. The results were shown in FIG. 9, where an Average nucleotide identity (ANI) value was: 98.39%, which was greater than an ANI same-species determination threshold of 96%. After identification, the M-SHDS-22 was Bacillus amyloliquefaciens, named Bacillus amyloliquefaciens strain MHDS-1, and was deposited in the CCTCC in Wuhan University, China on Jul. 10, 2023, with a deposit number of CCTCC NO: M20231262.

Example 2
(1) Activation of the Strain:

The Bacillus amyloliquefaciens strain MHDS-1 was inoculated on LB solid medium and cultured at 32° C. for 38 h to obtain an activated Bacillus amyloliquefaciens strain MHDS-1.

The activation medium was an LB solid medium, including 10 g/L of peptone, 5 g/L of yeast extract, 10 g/L of sodium chloride, 15 g/L of agar, and water as a balance. Each component was dissolved by heating and then sterilized by high-pressure steam at 121° C. for 30 min.

(2) Preparation of Bacterial Suspension:

Distilled water was filled into 6 15 mL centrifuge tubes, of which a 1st centrifuge tube was filled with 10 mL of the distilled water, while 2nd to 6th centrifuge tubes were filled with 9 mL of the distilled water, and the tube lids were not fastened. 100 mL of distilled water was filled into a 250 mL wide-mouthed triangular flask, and a breathable sealing film was placed on a flask mouth. After high-pressure steam sterilization at 121° C. for 30 min, the flasks were all placed in a super clean bench to allow air cooling to obtain sterile water.

The activated strain was scraped with a sterile inoculation loop and placed in the sterile water, and a strain concentration was adjusted to 0.5×10⁸CFU/mL to obtain a bacterial suspension of the Bacillus amyloliquefaciens strain MHDS-1.

(3) Fermentation:

80 g of 80-mesh Taxus media