TECHNICAL FIELD OF THE INVENTION

The present invention relates to a triazine compound having a microsomal prostaglandin E2 synthase-1 (mPGES-1) inhibitory activity or a pharmaceutically acceptable salt thereof, a pharmaceutical composition containing same, pharmaceutical use thereof and the like.

BACKGROUND OF THE INVENTION

Non-steroidal anti-inflammatory drugs (NSAIDs) are often used for the treatment of diseases accompanying inflammation, fever and pain, for example, rheumatism, osteoarthritis, headache and the like. NSAIDs show an anti-inflammatory action, an antipyretic action and an analgesic action by preventing production of prostanoids by inhibiting cyclooxygenase (COX).

COX includes two isoforms of COX-1 which is ubiquitously distributed and constitutively expressed, and COX-2 which expression is induced by various pro-inflammatory stimulations, for example, cytokines such as interleukin-1Î² (IL-1Î²) and the like. COX-1 and COX-2 are enzymes that convert arachidonic acid derived from cell membrane phospholipids to prostaglandin H2 (PGH2) which is a prostanoid precursor. Specific prostanoid synthases are responsible for the conversion of PGH2 to respective prostanoids (prostaglandin E2 (PGE2), prostaglandin F2Î± (PGF2Î±), prostaglandin 12 (PGI2), prostaglandin D2 (PGD2), thromboxane A2 (TXA2) etc.). These prostanoids have various physiological activities, for example, induction/suppression of inflammation, vasodilation/vasoconstriction, bronchodilation/bronchoconstriction, induction of/awakening from sleep, development of fever and the like. PGE2 is the most commonly existing prostaglandin in living organisms, and is known to be deeply involved in inflammation, pain and fever. Therefore, suppression of PGE2 production is considered the main action mechanism of NSAIDs.

Inhibition of COX-1 or COX-2 suppresses all prostanoids production in the downstream thereof. This is considered to cause side effects of NSAIDs. Since NSAIDs that non-selectively inhibit COX also suppress production of PGE2 by COX-1 and PGE2 protectively acts on stomach mucosal injury, NSAIDs are considered to suppress secretion of gastric mucus and gastric mucosal blood flow, thereby increasing the risk of stomach perforations, bleeding and the like. While COX-2 selective inhibitors suppress production of PGI2 having a vasodilation action and a platelet aggregation inhibitory action in vascular endothelial cells, they do not suppress production of TXA2 which is a blood coagulation factor produced by platelet COX-1. Therefore, they are considered to disrupt the balance of the blood coagulation system to increase the risk of cardiovascular disorder.

Microsomal prostaglandin E2 synthase-1 (mPGES-1) is an enzyme that catalyzes the final step of PGE2 biosynthesis, and belongs to the membrane-associated proteins in eicosanoid and glutathione metabolism family (MAPEG family). The human mPGES-1 gene was cloned in 1999, and indicated to be constitutively expressed in placenta, prostate, testis and mammary gland (non-patent document 1). In other organs, human mPGES-1 gene expression is induced by various pro-inflammatory stimulations, conjugated with COX-2. For example, inflammatory cytokine IL-1Î² and Tumor Necrosis Factor-Î± (TNF Î±) induce mPGES-1 expression in synovial cell, osteoblast, endothelial cell, orbital fibroblast, gingival cell, chondrocyte, endothelial cell, myocardial cell and the like. For example, Lipopolysaccharide (LPS), which is a bacterial endotoxin, induces mPGES-1 expression in macrophage, smooth muscle and the like.

mPGES-1 inhibitor is considered to selectively suppress PGE2 production only in the topical site of inflammation or tissues where mPGES-1 is expressed, and does not suppress production of prostanoids (PGI2, PGD2, PGF2Î±, TXA2 etc.) other than PGE2 (non-patent documents 2, 3). Therefore, mPGES-1 inhibitor is considered to be a medicament having an efficacy equivalent to that of NSAIDs but free of side effects of NSAIDs derived from a decreased production of prostanoids other than PGE2.

It is also known that when one of the metabolism pathways downstream from PGH2 is shut off in the arachidonic acid cascade, PGH2 is converted to prostanoids other than the shut-off pathway, or shunt occurs. That is, it is known that while the production amount of PGE2 in macrophage derived from mPGES-1 knockout mice stimulated with LPS becomes lower than the PGE2 production amount in macrophage derived from wild-type (WT) mice stimulated with LPS, the production amounts of TXB2, PGI2, PGD2 and PGF2Î± in macrophage derived from mPGES-1 knockout mice stimulated with LPS increase beyond the production amounts thereof in macrophage derived from WT mice stimulated with LPS (non-patent document 4). Since mPGES-1 inhibitor increases production of other prostanoids while suppressing the PGE2 production, it is considered to be effective even for diseases different from those treated by NSAIDs.

Use of mPGES-1 inhibitor is described below.

(1) Pain

In mPGES-1 knockout mice, intraperitoneal PGE2 production amount and nociceptive response per unit time significantly decrease as compared to WT mice, in the evaluation of nociceptive response by LPS stimulation which is an acute inflammatory pain model. Therefore, mPGES-1 inhibitor is considered to be an analgesic for acute inflammatory pain (non-patent documents 3, 6).

(2) Rheumatism

mPGES-1 gene of Swedish females contains some single nucleotide polymorphisms that increase the onset risk and severity of rheumatism. An increase in the mPGES-1 expression is immunohistologically confirmed in the synovium of rheumatism patients showing single nucleotide polymorphism (Reference SNP ID number: rs23202821) that increases severity, as compared to patients free of mutation (non-patent document 5). In mPGES-1 knockout mice, intraarticular infiltration of inflammatory cells, articular destruction and tumentia of the four limbs are markedly suppressed in a collagen-induced arthritis model, which is an animal model of rheumatism, as compared to WT mice (non-patent document 6). Therefore, mPGES-1 inhibitor is considered to be a therapeutic drug for rheumatism.

(3) Osteoarthritis

mRNA expression of mPGES-1 increases in meniscus cells of osteoarthritis patients (non-patent document 7). mPGES-1 inhibitor reduces nociceptive responses in osteoarthritis model using monoiodoacetic acid, as compared to WT mice (patent document 1). Therefore, mPGES-1 inhibitor is considered to be a therapeutic drug for osteoarthritis.

(4) Fever

In mPGES-1 knockout mice, body temperature elevation due to LPS stimulation is suppressed as compared to WT mice (non-patent document 8). Therefore, mPGES-1 inhibitor is considered to be an antipyretic drug.

(5) Alzheimer's Disease

Long-term use of NSAIDs mitigates the onset and progression of Alzheimer's disease. Under amyloid Î² peptide treatment, PGE2 production in the primary culture brain neuron of mPGES-1 knockout mice is suppressed, compared to the brain neuron of WT mice, and nerve cell death does not occur (non-patent document 9). Therefore, mPGES-1 inhibitor is considered to be a therapeutic drug for Alzheimer's disease.

(6) Multiple Sclerosis

EP4 gene of multiple sclerosis patients contains some single nucleotide polymorphisms that increase the onset risk (Reference SNP ID numbers: rs9292777, rs4613763, rs1044063, rs6896969). In macrophage present in the periventricular demyelinating lesion of multiple sclerosis patients, expression of mPGES-1 protein is confirmed. In mPGES-1 knockout mice, PGE2 production in the spinal cord of experimental autoimmune encephalomyelitis model mice, which is an animal model of multiple sclerosis, is suppressed, and progression of paralysis is suppressed, as compared to WT mice, (non-patent document 10) Therefore, mPGES-1 inhibitor is considered to be a therapeutic drug for multiple sclerosis.

(7) Arteriosclerosis

In mPGES-1 knockout mice, PGE2 production in vascular endothelial cells of high-fat fed low density lipoprotein (LDL) receptor deficient mice, which is an atherosclerosis model, decreases, and atheroma formation is delayed as compared to WT mice. In vascular endothelial cells, production of PGI2, which is known to have a platelet function suppressive action, increases (non-patent document 11). Therefore, mPGES-1 inhibitor is considered to be a prophylactic or therapeutic drug for arteriosclerosis.

(8) Glaucoma, Ocular Hypertension

Glaucoma is a disease showing a characteristic change in the optic nerve and the field of vision. Optic nerve disorder can be generally improved or suppressed by sufficiently decreasing the intraocular pressure. Glaucoma can be categorized into open angle glaucoma and closed angle glaucoma.

mPGES-1 gene is constitutively highly expressed in human conjunctiva (GEO accession No: GSE2513 (Gene Expression Omnibus:http://www.ncbi.nlm.nih.gov/geo/)). In the retina of glaucoma patients, expression of mPGES-1 increases as compared to healthy individuals. In the retina of high intraocular pressure dogs and high intraocular pressure mice, which are glaucoma models, expression of mPGES-1 increases as compared to normal animals (GEO accession No: human GSE2378, dog GSE21879, mouse GSE3554).

When PGE2 is instilled into the eyes of healthy individuals, the intraocular pressure increases, along with the expansion of blood vessels, for 2 hours after instillation (non-patent document 12). When PGE2 is administered to rabbits subconjunctivally, the intraocular pressure increases due to dilatation of ciliary body and increase in the aqueous humor production (non-patent document 13). PGF2Î± and PGD2, which are prostaglandins that may increase when mPGES-1 is inhibited, decrease the intraocular pressure of rabbit (non-patent document 14). PGF2Î± formulations increase outflow of aqueous humor and are used as therapeutic drugs for glaucoma that decrease the intraocular pressure. PGI2 does not show a clear action on the intraocular pressure of rabbits. That is, the intraocular pressure is considered to decrease since decrease of PGE2 suppresses aqueous humor production by mPGES-1 inhibition, and/or since increased PGD2 and PGF2Î± promote outflow of aqueous humor due to shunt. Also, PGE2 promotes expression of vascular endothelial growth factor (VEGF) from retina (non-patent document 15). Since VEGF produced in retina transfers to the anterior ocular segment to cause angiogenesis glaucoma, which is increase of the intraocular pressure that is caused by obstruction of corner angle due to angiogenesis in iris, mPGES-1 inhibitor is considered to show an improvement or prophylactic effect on angiogenesis glaucoma as well. Furthermore, considering an anti-inflammatory action by the inhibition of PGE2 production, mPGES-1 inhibitor is applicable to patients having intraocular inflammation, who require careful administration of the existing prostaglandin formulations (latanoprost etc.). Therefore, mPGES-1 inhibitor is considered to be a therapeutic drug also effective for glaucoma having various background diseases.

(9) Ischemic Retinal Disease

Excessive secretion of VEGF plays a key role in ischemic retinal diseases such as diabetic retinopathy, diabetic macular edema, retinal vein occlusion and the like. Since PGE2 promotes expression of VEGF (non-patent document 15), mPGES-1 inhibitor is considered to improve these diseases.

(10) Systemic Scleroderma

Expression of mPGES-1 increases in the skin of systemic scleroderma patients, as compared to healthy individuals. Similarly, expression of mPGES-1 increases in the skin of bleomycin induced scleroderma model mice, which is a systemic scleroderma model, as compared to the skin of normal mice. As compared to WT mice, mPGES-1 knockout mice showed a decrease in the accumulation of macrophage in the dermal lesion of bleomycin induced scleroderma model mice, and mitigation of cutaneous thickening, deposition of extracellular matrix and increase in the collagen content (non-patent document 16). Therefore, mPGES-1 inhibitor is considered to be a therapeutic drug for systemic scleroderma.

(11) Cancer

In mPGES-1 knockout mice, the polyp number and size were markedly suppressed in azoxymethane-induced colorectal cancer model mice, which are animal model of colorectal cancer, as compared to WT mice. In mPGES-1 knockout mice, PGE2 production in large intestinal tumor tissue decreased and production amount of PGI2 that inhibits adhesion of cancer cells and PGD2 that induces cell death via peroxisome proliferator-activated receptor Î³ (PPARÎ³) increased, as compared to WT mice. When colorectal cancer or lung cancer cells were transplanted into the spleen of mPGES-1 knockout mice, the post-transplantation weight of spleen tumor and the rate of metastasis to the liver decreased as compared to WT mice. Growth of lung cancer cells was decreased when they ware co-cultured in vitro with mPGES-1 knockout mice-derived bone marrow macrophages compared to when they ware co-cultured with WT mice-derived bone marrow macrophages, which indicates that host macrophage-derived PGE2 is involved in cancer cell growth (non-patent document 17).

Therefore, mPGES-1 inhibitor is considered to be an anticancer drug that suppresses the growth and metastasis of cancer including colorectal cancer.

(12) Disease for which Suppression of PGE2 Production is Effective

As inflammatory symptoms and/or pain relating to the conditions thereof, for which NSAIDs are effective, for example, arthritis, gout, nephrolithiasis, urolithiasis, headache, menstrual pain, toothache, lumbago, muscular pain, periarthritis scapulohumeralis, cervical syndrome, temporomandibular disorder, and postoperative or posttraumatic inflammation and pain, and inflammation and pain after tooth extraction can be mentioned. Besides these, acute and chronic non-bacterial inflammation of eye can be mentioned and, for example, uveitis, allergic conjunctivitis and postoperative inflammation and ophthalmalgia in intraocular operation can be mentioned.

The main mechanism for the efficacy of NSAIDs is considered to be the suppression of PGE2 production, which is an inflammation promoting substance. Since mPGES-1 inhibitor also has a suppressive action on the PGE2 production, it is considered to be a therapeutic drug for these diseases.

The mPGES-1 inhibitor is considered to be beneficial for the prophylaxis or treatment of pain, rheumatism, osteoarthritis, fever, Alzheimer's disease, multiple sclerosis, arteriosclerosis, glaucoma, ocular hypertension, ischemic retinal disease, systemic scleroderma, cancer including colorectal cancer and diseases for which suppression of PGE2 production is effective.

DOCUMENT LISTPatent Document

patent document 1: WO 2012/161965

Non-Patent Documents

non-patent document 1: JAKOBSSON, P J et al. Identification of human prostaglandin E synthase: a microsomal, glutathione-dependent, inducible enzyme, constituting a potential novel drug target. Proc Natl Acad Sci USA. Jun. 22 1999, Vol. 96, No. 13, pages 7220-7225.
non-patent document 2: SAMUELSSON, B et al. Membrane prostaglandin E synthase-1: a novel therapeutic target. Pharmacol Rev. Sep. 2007, Vol. 59, No. 3, pages 207-224.
non-patent document 3: KAMEI, D et al. Reduced pain hypersensitivity and inflammation in mice lacking microsomal prostaglandin e synthase-1. J Biol Chem. Aug. 6 2004, Vol. 279, No. 32, pages 33684-33695.
non-patent document 4: TREBINO, C E et al. Redirection of eicosanoid metabolism in mPGES-1-deficient macrophages. J Biol Chem. Apr. 29 2005, Vol. 280, No. 17, pages 16579-16585.
non-patent document 5: KOROTKOVA, M et al. Variants of gene for microsomal prostaglandin E2 synthase show association with disease and severe inflammation in rheumatoid arthritis. Eur J Hum Genet. Aug. 2011, Vol. 19, No. 8, pages 908-914.
non-patent document 6: TREBINO, C E et al. Impaired inflammatory and pain responses in mice lacking an inducible prostaglandin E synthase. Proc Natl Acad Sci USA. Jul. 22 2003, Vol. 100, No. 15, pages 9044-9049.
non-patent document 7: SUN, Y et al. Analysis of meniscal degeneration and meniscal gene expression. BMC Musculoskelet Disord. 2010, Vol. 11, pages 19.
non-patent document 8: ENGBLOM, D et al. Microsomal prostaglandin E synthase-1 is the central switch during immune-induced pyresis. Nat Neurosci. Nov. 2003, Vol. 6, No. 11, pages 1137-1138.
non-patent document 9: KUROKI, Y et al. Deletion of microsomal prostaglandin E synthase-1 protects neuronal cells from cytotoxic effects of beta-amyloid peptide fragment 31-35. Biochem Biophys Res Commun. Aug. 3 2012, Vol. 424, No. 3, pages 409-413.
non-patent document 10: KIHARA, Y et al. Targeted lipidomics reveals mPGES-1-PGE2 as a therapeutic target for multiple sclerosis. Proc Natl Acad Sci USA. Dec. 22 2009, Vol. 106, No. 51, pages 21807-21812.
non-patent document 11: WANG, M et al. Deletion of microsomal prostaglandin E synthase-1 augments prostacyclin and retards atherogenesis. Proc Natl Acad Sci USA. Sep. 26 2006, Vol. 103, No. 39, pages 14507-14512.
non-patent document 12: FLACH, A J et al. Topical prostaglandin E2 effects on normal human intraocular pressure. J Ocul Pharmacol. Spring 1988, Vol. 4, No. 1, pages 13-18.
non-patent document 13: NAKAJIMA, T et al. [Effects of prostaglandin E2 on intraocular pressure, anterior chamber depth and blood flow volume of the iris and the ciliary body in rabbit eyes]. Nihon Ganka Gakkai Zasshi. Apr. 1992, Vol. 96, No. 4, pages 455-461.
non-patent document 14: GOH, Y et al. Prostaglandin D2 reduces intraocular pressure. Br J Ophthalmol. Jun. 1988, Vol. 72, No. 6, pages 461-464.
non-patent document 15: YANNI, S E et al. The role of PGE2 receptor EP4 in pathologic ocular angiogenesis. Invest Ophthalmol Vis Sci. Nov. 2009, Vol. 50, No. 11, pages 5479-5486.
non-patent document 16: MCCANN, M R et al. mPGES-1 null mice are resistant to bleomycin-induced skin fibrosis. Arthritis Res Ther. 2011, Vol. 13, No. 1, pages R6.
non-patent document 17: SASAKI, Y et al. Microsomal prostaglandin E synthase-1 is involved in multiple steps of colon carcinogenesis. Oncogene. Jun. 14 2012, Vol. 31, No. 24, pages 2943-2952.

SUMMARY OF THE INVENTION

The present invention aims to provide a triazine compound having an mPGES-1 inhibitory activity or a pharmaceutically acceptable salt thereof, a pharmaceutical composition containing same, and pharmaceutical use thereof and the like. As the target disease, for example, pain, rheumatism, osteoarthritis, fever, Alzheimer's disease, multiple sclerosis, arteriosclerosis, glaucoma, ocular hypertension, ischemic retinal disease, systemic scleroderma, cancer including colorectal cancer and diseases for which suppression of PGE2 production is effective can be mentioned.

The present inventors have found a triazine compound having an mPGES-1 inhibitory activity, which is represented by the following formula [I], and completed the present invention.

Accordingly, the present invention is as follows.

[01]

A compound represented by the formula [I] or a pharmaceutically acceptable salt thereof:

[Image Omitted]

wherein

X is CH or N,

ring Cy is
the formula:

[Image Omitted]

or
the formula:

[Image Omitted]

{wherein R¹is

- (1) halogen,
- (2) C_1-6alkyl,
- (3) cyano or
- (4) haloC_1-4alkyl,

R²is

- (1) halogen,
- (2) hydroxy,
- (3) carboxy,
- (4) C_1-6alkyl,
- (5) C_1-6alkoxy,
- (6) haloC_1-4alkoxy,
- (7) haloC_1-4alkyl,
- (8) C_1-6alkyl-carbonyl,
- (9) â€”C(O)NR^a1R^a2(R^a1and R^a2are each independently hydrogen or C_1-6alkyl) or
- (10) â€”(C_nH_2n)â€”R^b
- (n is 1, 2, 3 or 4, â€”(C_nH_2n)â€” may be straight or branched chain, and

R^bis

- (a) hydroxy,
- (b) carboxy,
- (c) C_1-6alkoxy,
- (d) C_1-6alkyl-carbonyloxy,
- (e) â€”C(O)NR^b1R^b2(R^b1and R^b2are each independently hydrogen or C_1-6alkyl),
- (f) â€”OC(O)NR^b3R^b4(R^b3and R^b4are each independently hydrogen or C_1-6alkyl),
- (g) â€”NR^b5C(O)NR^b6R^b7(R^b5, R^b6and R^b7are each independently hydrogen or C_1-6alkyl),
- (h) â€”NR^b8R^b9(R^b8and R^b9are each independently hydrogen, C_1-6alkyl or haloC_1-4alkyl),
- (i) â€”NR^b10S(O)₂R^b11(R^b10and R^b11are each independently hydrogen, C_1-6alkyl or C_3-7cycloalkyl),
- (j) â€”NR^b12C(O)OR^b13(R^b12is hydrogen or C_1-6alkyl, and R^b13is C_1-6alkyl),
- (k) â€”NR^b14C(O)R^b15(R^b14is hydrogen or C_1-6alkyl, and

R^b15is

- (i) C_6-10aryl,
- (ii) C_1-8alkyl (said C_1-8alkyl is optionally substituted by 1, 2 or 3 substituents selected from the group consisting of hydroxy, haloC_1-4alkyl, C_1-6alkoxy and C_6-10aryl),
- (iii) adamantyl or
- (iv) C_3-7cycloalkyl (said C_3-7cycloalkyl is optionally substituted by 1, 2, 3 or 4 substituents selected from the group consisting of C_1-6alkyl, halogen, hydroxyl C_1-6alkyl and halo C_1-4alkyl, and/or optionally form a fused ring with a benzene ring), or

R^b14and R^b15optionally form a 4-, 5- or 6-membered lactam together with the nitrogen atom the nitrogen atom that R^b14is bonded to and the carbon atom that R^b15is bonded to (said lactam is optionally substituted by 1, 2 or 3 C_1-6alkyls, and/or optionally form a fused ring with a benzene ring),

- (l) the formula:

[Image Omitted]

wherein m2 and m3 are each independently 1, 2 or 3, m4 is 0, 1, 2, 3 or 4, R^b16is C_1-6alkyl or C_1-6alkoxy, and when m4 is 2, 3 or 4, each R^b16is selected independently, or

- (m) the formula:

[Image Omitted]

wherein m5 and m6 are each independently 1, 2 or 3, and R^b17is C_1-6alkyl or C_1-6alkoxy)),

R³is

- (1) halogen,
- (2) hydroxy,
- (3) C_1-6alkyl or
- (4) â€”OR^c{R^cis C_1-6alkyl optionally substituted by 1, 2 or 3 substituents selected from the group consisting of the following (a) to (f);
  - (a) halogen,
  - (b) hydroxy,
  - (c) C_1-6alkoxy,
  - (d) â€”C(O)NR^c1R^c2(R^c1and R^c2are each independently hydrogen or C_1-6alkyl),
  - (e) C_6-10aryl (said C_6-10aryl is optionally substituted by 1, 2 or 3 substituents selected from the group consisting of
    - (i) halogen,
    - (ii) hydroxy,
    - (iii) C_1-6alkyl,
    - (iv) C_1-6alkoxy, and
    - (v) haloC_1-4alkyl), and
  - (f) 5- or 6-membered heteroaryl containing 1, 2 or 3 nitrogen atoms, oxygen atoms or sulfur atoms (said heteroaryl is optionally substituted by 1, 2 or 3 substituents selected from the group consisting of
    - (i) halogen,
    - (ii) hydroxy,
    - (iii) C_1-6alkyl,
    - (iv) C_1-6alkoxy, and
    - (v) haloC_1-4alkyl)}, and

R⁴is

- (1) hydrogen,
- (2) halogen,
- (3) C_1-6alkyl or
- (4) C_1-6alkoxy},

R⁵is

- (1) halogen,
- (2) hydroxy,
- (3) C_1-6alkylsulfanyl,
- (4) C_1-6alkyl (said C_1-6alkyl is optionally substituted by 1, 2 or 3 substituents selected from the group consisting of halogen, C_6-10aryl and C_1-6alkoxy),
- (5) C_3-7cycloalkyl,
- (6) â€”OR^d{R^dis
  - (a) C_2-6alkynyl,
  - (b) C_3-7cycloalkyl optionally substituted by 1, 2 or 3 C_1-6alkyls or
  - (c) C_1-8alkyl (said C_1-8alkyl is optionally substituted by 1, 2 or 3 substituents selected from the group consisting of the following (i) to (v);
    - (i) halogen,
    - (ii) C_6-10aryl,
    - (iii) C_1-6alkoxy,
    - (iv) C_3-7cycloalkyl (said C_3-7cycloalkyl is optionally substituted by 1, 2 or 3 substituents selected from the group consisting of C_1-6alkyl and haloC_1-4alkyl), and
    - (v) 4-, 5- or 6-membered saturated heterocyclyl containing 1, 2 or 3 nitrogen atoms, oxygen atoms or sulfur atoms (said saturated heterocyclyl is optionally substituted by 1, 2 or 3 substituents selected from the group consisting of C_1-6alkyl and haloC_1-4alkyl))} or
- (7) the formula:

[Image Omitted]

wherein R^eis

- (a) C_1-6alkyl,
- (b) C_3-7cycloalkyl,
- (c) 5- or 6-membered heteroaryl containing 1, 2 or 3 nitrogen atoms, oxygen atoms or sulfur atoms, or
- (d) C_6-10aryl (said C_6-10aryl is optionally substituted by 1, 2 or 3 substituents selected from the group consisting of
  - (i) halogen,
  - (ii) C_1-6alkyl,
  - (iii) haloC_1-4alkyl,
  - (iv) C_1-6alkoxy, and
  - (v) haloC_1-4alkoxy), and
    m1 is 0, 1, 2 or 3 and, when m1 is 2 or 3, each R⁵is selected independently,
    excluding 4,6-bis-(2,5-dimethyl-phenyl)-1,3,5-triazin-2-ol.
    [02]

The compound of [01] or a pharmaceutically acceptable salt thereof, wherein ring Cy is the formula:

[Image Omitted]

wherein R¹, R²and R⁴are as defined in [01].
[03]

The compound of [01] or a pharmaceutically acceptable salt thereof, wherein ring Cy is the formula:

[Image Omitted]

wherein R¹, R³and R⁴are as defined in [01]
[04]

The compound of any of [01] to [03] or a pharmaceutically acceptable salt thereof, wherein X is CH.

[05]

The compound of any of [01] to [03] or a pharmaceutically acceptable salt thereof, wherein X is N.

[06]

The compound of any of [01] to [05] or a pharmaceutically acceptable salt thereof, wherein R¹is

- (1) chloro,
- (2) methyl,
- (3) cyano or
- (4) trifluoromethyl.
  [07]

The compound of any of [01] to [06] or a pharmaceutically acceptable salt thereof, wherein R⁴is hydrogen.

[08]

The compound of any of [01], [02] and [04] to [07] or a pharmaceutically acceptable salt thereof, wherein R²is â€”(C_nH_2n)â€”R^b(n is 1 or 2, â€”(C_nH_2n)â€” may be straight or branched chain, and R^bis

- (a) â€”C(O)NR^b1R^b2,
- (b) â€”NR^b5C(O)NR^b6R^b7,
- (c) â€”NR^b10S(O)₂R^b11or
- (d) â€”NR^b14C(O) R^b15
  (R^b1, R^b2, R^b5, R^b6, R^b7, R^b10, R^b11, R^b14, and R^b15are as defined in [01])).
  [09]

The compound of [08] or a pharmaceutically acceptable salt thereof, wherein R²is â€”CH₂â€”R^b(R^bis as defined in [08])

[10]

The compound of any of [01] and [03] to [09] or a pharmaceutically acceptable salt thereof, wherein R³is

- (1) halogen,
- (2) hydroxy,
- (3) C_1-6alkyl or
- (4) â€”OR^c{R^cis C_1-6alkyl optionally substituted by 1, 2 or 3 substituents selected from the group consisting of the following (a) to (f)
  - (a) halogen,
  - (b) hydroxy,
  - (c) C_1-6alkoxy,
  - (d) â€”C(O)NR^c1R^c2(R^c1and R^c2are each independently hydrogen or C_1-6alkyl),
  - (e) phenyl (said phenyl is optionally substituted by 1, 2 or 3 substituents selected from the group consisting of
    - (i) halogen,
    - (ii) hydroxy,
    - (iii) C_1-6alkyl,
    - (iv) C_1-6alkoxy, and
    - (v) haloC_1-4alkyl), and
  - (f) pyridyl (said pyridyl is optionally substituted by 1, 2 or 3 substituents selected from the group consisting of
    - (i) halogen,
    - (ii) hydroxy,
    - (iii) C_1-6alkyl,
    - (iv) C_1-6alkoxy, and
    - (v) haloC_1-4alkyl)}.
      [11]

The compound of any of [01] to [10] or a pharmaceutically acceptable salt thereof, wherein m1 is 1, and R⁵is the formula:

[Image Omitted]

wherein R^eis as defined in [01]
[12]

A compound selected from the following formulas:

[Image Omitted]

or a pharmaceutically acceptable salt thereof.
[13]

A pharmaceutical composition comprising the compound of any of [01] to [12] or a pharmaceutically acceptable salt thereof, and a pharmaceutically acceptable carrier.

[14]

An mPGES-1 inhibitor comprising the compound of any of [01] to [12] or a pharmaceutically acceptable salt thereof.

[15]

A therapeutic or prophylactic agent for pain, rheumatism, fever, osteoarthritis, arteriosclerosis, Alzheimer's disease, multiple sclerosis, glaucoma, ocular hypertension, ischemic retinal disease, systemic scleroderma, cancer or a disease for which suppression of PGE2 production is effective, comprising the compound of any of [01] to [12] or a pharmaceutically acceptable salt thereof.

[16]

A therapeutic or prophylactic agent for glaucoma or ocular hypertension, comprising the compound of any of [01] to [12] or a pharmaceutically acceptable salt thereof, and one or more kinds of other therapeutic agents for glaucoma in combination.

[17]

A method of inhibiting mPGES-1, comprising administering a pharmaceutically effective amount of the compound of any of [01] to [12] or a pharmaceutically acceptable salt thereof to a human.

[18]

A method of treating or preventing pain, rheumatism, fever, osteoarthritis, arteriosclerosis, Alzheimer's disease, multiple sclerosis, glaucoma, ocular hypertension, ischemic retinal disease, systemic scleroderma, cancer or a disease for which suppression of PGE2 production is effective, which method comprising administering a pharmaceutically effective amount of the compound of any of [01] to [12] or a pharmaceutically acceptable salt thereof to a human.

[19]

The method of [18] for treating or preventing glaucoma or ocular hypertension, further comprising administering a pharmaceutically effective amount of one or more kinds of other therapeutic agents for glaucoma to the human.

[20]

Use of the compound of any of [01] to [12] or a pharmaceutically acceptable salt thereof for the production of an mPGES-1 inhibitor.

[21]

Use of the compound of any of [01] to [12] or a pharmaceutically acceptable salt thereof for the production of a therapeutic or prophylactic agent for pain, rheumatism, fever, osteoarthritis, arteriosclerosis, Alzheimer's disease, multiple sclerosis, glaucoma, ocular hypertension, ischemic retinal disease, systemic scleroderma, cancer or a disease for which suppression of PGE2 production is effective.

Effect of the Invention

The compound of the present invention is effective as a therapeutic or prophylactic agent for pain, rheumatism, fever, osteoarthritis, arteriosclerosis, Alzheimer's disease, multiple sclerosis, glaucoma, ocular hypertension, ischemic retinal disease, systemic scleroderma, cancer including colorectal cancer, a disease for which suppression of PGE2 production is effective and the like.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1 shows effect of a test article (compounds of Example 2-98), a reference article (Xalatan (registered trademark)) or a vehicle (methylcellulose, MC) on the intraocular pressure immediately before and after administration in Macaca fascicularis.

DESCRIPTION OF EMBODIMENTS

The definitions of the terms used in the present invention are as follows.

The â€œhalogenâ€ is fluoro, chloro, bromo or iodo.

The â€œC_1-6alkylâ€ means straight chain or branched chain alkyl having 1 to 6 carbon atoms. Examples thereof include methyl, ethyl, propyl, isopropyl, butyl, isobutyl, sec-butyl, tert-butyl, pentyl, isopentyl, neopentyl, 1-ethylpropyl, hexyl, isohexyl, 1,1-dimethylbutyl, 2,2-dimethylbutyl, 3,3-dimethylbutyl, 2-ethylbutyl and the like.

The â€œC_1-8alkylâ€ means straight chain or branched chain alkyl having 1 to 8 carbon atoms. Examples thereof include methyl, ethyl, propyl, isopropyl, 1,1-dimethylpropyl, 1-ethyl-propyl, l-methyl-1-ethyl-propyl, butyl, isobutyl, sec-butyl, tert-butyl, 1-methyl-1-propyl-butyl, pentyl, isopentyl, neopentyl, 1-ethylpropyl, hexyl, isohexyl, 1,1-dimethylbutyl, 2,2-dimethylbutyl, 3,3-dimethylbutyl, 2-ethylbutyl and the like.

The â€œC_1-6alkoxyâ€ means alkoxy wherein the alkyl moiety is the above-defined â€œC_1-6alkylâ€ . Examples thereof include methoxy, ethoxy, propoxy, isopropoxy, butoxy, isobutoxy, sec-butoxy, tert-butoxy, pentyloxy, isopentyloxy, neopentyloxy, 1,2-dimethylpropyloxy, 1-ethylpropyloxy, hexyloxy, isohexyloxy, 1,2,2-trimethylpropyloxy, 1,1-dimethylbutyloxy, 2,2-dimethylbutyloxy, 3,3-dimethylbutyloxy, 2-ethylbutyloxy and the like.

The â€œhaloC_1-4alkylâ€ means straight chain or branched chain alkyl having 1-4 carbon atoms, which is substituted by 1 to 9 the above-defined â€œhalogensâ€ . When it is substituted by plural halogens, respective halogens may be the same or different. Examples thereof include 2-fluoroethyl, 2-chloroethyl, 2-bromoethyl, 3-fluoropropyl, 3-chloropropyl, 4-fluorobutyl, 4-chlorobutyl, 1,1-difluoroethyl, 1,1-difluoropropyl, 1,1-difluoro-2-methylpropyl, trifluoromethyl, 2,2,2-trifluoroethyl, 3,3,3-trifluoropropyl, 4,4,4-trifluorobutyl, pentafluoroethyl, 2,2,2-trifluoro-1-trifluoromethyl-ethyl and the like.

The â€œhaloC_1-4alkoxyâ€ means alkoxy wherein the alkyl moiety is the above-defined â€œhaloC_1-4alkylâ€ . Examples thereof include fluoromethoxy, chloromethoxy, bromomethoxy, 2-fluoroethoxy, 2-chloroethoxy, 2-bromoethoxy, 3-fluoropropoxy, 3-chloropropoxy, 4-fluorobutoxy, 4-chlorobutoxy, 1,1-difluoroethoxy, 2,2-difluoroethoxy, 1,1-difluoropropoxy, 2,2-difluoropropoxy, 3,3-difluoropropoxy, 1,1-difluoro-2-methylpropoxy, trifluoromethoxy, 2,2,2-trifluoroethoxy, 3,3,3-trifluoropropoxy, 4,4,4-trifluorobutoxy, pentafluoroethoxy, 2,2,2-trifluoro-1-trifluoromethyl-ethoxy and the like.

The â€œhydroxyC_1-6alkylâ€ means the above-defined â€œC_1-6alkylâ€ substituted by 1 or 2 hydroxy. Examples thereof include hydroxymethyl, 2-hydroxyethyl, 1-hydroxy-1-methylethyl, 1,2-dihydroxyethyl, 3-hydroxypropyl, 1-hydroxy-2,2-dimethylpropyl, 4-hydroxybutyl, 1-hydroxy-2,2-dimethylbutyl, 5-hydroxypentyl, 6-hydroxyhexyl and the like.

The â€œC_1-6alkyl-carbonylâ€ means carbonyl bonded to the above-defined â€œC_1-6alkylâ€ . Examples thereof include acetyl, propionyl, 2,2-dimethylpropionyl, butyryl, 3-methylbutyryl, 2,2-dimethylbutyryl, pentanoyl, 4-methylpentanoyl, hexanoyl and the like.

The â€œC_1-6alkyl-carbonyloxyâ€ means carbonyloxy bonded to the above-defined â€œC_1-6alkylâ€ . Examples thereof include methylcarbonyloxy, ethylcarbonyloxy, propylcarbonyloxy, isopropylcarbonyloxy, butylcarbonyloxy, isobutylcarbonyloxy, sec-butylcarbonyloxy, tert-butylcarbonyloxy, pentylcarbonyloxy, isopentylcarbonyloxy, 2-methylbutylcarbonyloxy, 1,1-dimethylpropylcarbonyloxy, neopentylcarbonyloxy, 3,3-dimethylbutylcarbonyloxy, 1-ethylpropylcarbonyloxy, hexylcarbonyloxy and the like.

The â€œC_3-7cycloalkylâ€ means 3- to 7-membered monocyclic cycloalkyl. Examples thereof include cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl and cycloheptyl.

The â€œC_6-10arylâ€ means 6- to 10-membered aryl. Examples thereof include phenyl, 1-naphthyl, 2-naphthyl and the like. Of these, preferred is phenyl.

The â€œ5- or 6-membered heteroaryl containing 1, 2 or 3 nitrogen atoms, oxygen atoms or sulfur atomsâ€ means 5- or 6-membered monocyclic heteroaryl containing, besides carbon atoms, 1, 2 or 3 hetero atoms selected from a nitrogen atom, an oxygen atom and a sulfur atom. Examples thereof include furyl, thienyl, pyrrolyl, oxazolyl, isoxazolyl, thiazolyl, isothiazolyl, imidazolyl, pyrazolyl, oxadiazolyl(1,2,5-oxadiazolyl, 1,3,4-oxadiazolyl, 1,2,4-oxadiazolyl), thiadiazolyl(1,2,5-thiadiazolyl, 1,3,4-thiadiazolyl, 1,2,4-thiadiazolyl), triazolyl(1,2,3-triazolyl, 1,2,4-triazolyl), pyridyl, pyrimidinyl, pyridazinyl, pyrazinyl, 1,3,5-triazinyl and the like. Of these, preferred is pyridyl.

The â€œ4-, 5- or 6-membered saturated heterocyclyl containing 1, 2 or 3 nitrogen atoms, oxygen atoms or sulfur atomsâ€ means 4-, 5- or 6-membered monocyclic saturated heterocyclyl containing, besides carbon atoms, 1, 2 or 3 hetero atoms selected from a nitrogen atom, an oxygen atom and a sulfur atom. The carbon atom of the heterocycle is optionally substituted by oxo. When a sulfur atom is contained as a hetero atom, the sulfur atom is optionally monooxidized or dioxidized. Examples thereof include oxetanyl, azetidinyl, tetrahydrofuryl, tetrahydropyranyl, tetrahydrothienyl, tetrahydrothiopyranyl, oxazolidinyl, isoxazolidinyl, thiazolidinyl, isothiazolidinyl, imidazolidinyl, pyrazolidinyl, pyrrolidinyl, piperidyl (including piperidino), morpholinyl (including morpholino), thiomorpholinyl (including thiomorpholino), piperazinyl, 1,1-dioxidoisothiazolidinyl, 1,1-dioxidotetrahydrothienyl, 1,1-dioxidotetrahydrothiopyranyl, 1,1-dioxidothiomorpholinyl (including 1,1-dioxidothiomorpholino) and the like. In addition, the saturated heterocyclyl may be partially saturated. Examples thereof include imidazolinyl, oxazolinyl, pyrazolinyl, thiazolinyl and the like. Of these, preferred is oxetanyl.

The â€œC_1-6alkylsulfanylâ€ means sulfanyl bonded to the above-defined â€œC_1-6alkylâ€ . Examples thereof include methylsulfanyl, ethylsulfanyl, propylsulfanyl, isopropylsulfanyl, n-butylsulfanyl, isobutylsulfanyl, sec-butylsulfanyl, tert-butylsulfanyl, pentylsulfanyl, 1,1-dimethylpropylsulfanyl, 2,2-dimethylpropylsulfanyl, hexylsulfanyl and the like.

The â€œC_2-6alkynylâ€ means straight chain or branched chain hydrocarbon having 2 to 6 carbon atoms and at least one triple bond. Examples thereof include ethynyl, 1-propynyl, 2-propynyl, 1-butynyl, 2-butynyl, 3-butynyl, 1-methyl-2-propynyl, 3,3-dimethylbutynyl (that is, 3,3-dimethylbut-1-ynyl) and the like.

The â€œâ€”(C_nH_2n)â€”â€ means straight chain or branched chain alkylene having n carbon atoms and 2n hydrogen atoms. Examples thereof include â€”CH₂â€”, â€”CH₂CH₂â€”, â€”CH(CH₃)â€”, â€”CH₂CH₂CH₂â€”, â€”C(CH₃)₂â€”, â€”CH(CH₃)CH₂â€” and the like.

When R²is (10) â€”(C_nH_2n)â€”R^band R^bis (k) â€”NR^b14C(O)R^b15, â€œ(ii) C_1-8alkyl (said C_1-8alkyl is optionally substituted by 1, 2 or 3 substituents selected from the group consisting of hydroxy, haloC_1-4alkyl, C_1-6alkoxy and C_6-10aryl)â€ for R^b15means the above-defined â€œC_1-8alkylâ€ substituted or not substituted by the same or different, 1, 2 or 3 substituents selected from the group consisting of hydroxy, the above-defined â€œhaloC_1-4alkylâ€ , the above-defined â€œC_1-6alkoxyâ€ and the above-defined â€œC_6-10arylâ€ , at the substitutable position(s) thereof. Examples of R^binclude 2-ethoxy-3-methoxypropylcarbonylamino, 1-methyl-1-methoxy-2,2,2-trifluoroethylcarbonylamino and the like.

When R²is (10) â€”(C_nH_2n)â€”R^band R^bis (k) â€”NR^b14C(O)R^b15, â€œ(iv) C_3-7cycloalkyl (said C_3-7cycloalkyl is optionally substituted by 1, 2, 3 or 4 substituents selected from the group consisting of C_1-6alkyl, halogen, hydroxyC_1-6alkyl and haloC_1-4alkyl, and/or optionally form a fused ring with a benzene ring)â€ for R^b15means (1) the above-defined â€œC_3-7cycloalkylâ€ substituted by the same or different, 1, 2, 3 or 4 substituents selected from the group consisting of the above-defined â€œC_1-6alkylâ€ , the above-defined â€œhalogenâ€ , the above-defined â€œhydroxyC_1-6alkylâ€ and the above-defined â€œhaloC_1-4alkylâ€ , at the substitutable position(s) thereof, (2) unsubstituted C_3-7cycloalkyl, or (3) C_3-7cycloalkyl of (1) or (2), fused with one benzene ring at a fusible position. Examples of R^binclude 1,2,3,4-tetrahydro-naphthalen-2-ylcarbonylamino, 2-methyl-indan-2-ylcarbonylamino and the like.

When R²is (10) â€”(C_nH_2n)â€”R^band R^bis (k) â€”NR^b14C(O)R^b15, â€œR^b14and R^b15optionally form a 4-, 5- or 6-membered lactam together with the nitrogen atom that R^b14is bonded to and the carbon atom that R^b15is bonded toâ€ means that R^bis 2-oxo-azetidin-1-yl, 2-oxo-pyrrolidin-1-yl, 2-oxo-piperidin-1-yl or the like.

In addition, in this case, â€œsaid lactam is optionally substituted by 1, 2 or 3 C_1-6alkyls, and/or optionally form a fused ring with a benzene ringâ€ means that, in addition to the above-mentioned â€œlactamâ€ , (1) the same or different 1, 2 or 3 C_1-6alkyls defined above are present at the substitutable position(s) of the lactam, (2) one benzene ring is fused at the fusible position of the lactam, and (3) one benzene ring is fused at the fusible position of the lactam substituted by C_1-6alkyl(s). Examples of R^binclude 3,4-dimethyl-2-oxo-pyrrolidin-1-yl, 1-oxo-1,3-dihydro-isoindol-2-yl, 3,3-dimethyl-2-oxo-2,3-dihydro-indol-1-yl and the like.

In the compound represented by the formula [I], preferable embodiments of respective groups are as described below.

R¹is preferably chloro, methyl, cyano or trifluoromethyl, more preferably chloro or trifluoromethyl, and further preferably chloro.

R²is preferably

- (1) halogen,
- (2) hydroxy,
- (3) carboxy,
- (5) C_1-6alkoxy,
- (6) haloC_1-4alkoxy,
- (7) haloC_1-4alkyl,
- (8) C_1-6alkyl-carbonyl,
- (9) â€”C(O)NR^a1R^a2(R^a1and R^a2are as defined above) or
- (10) â€”(C_nH_2n)â€”R^b(R^bis as defined above), more preferably
- (10) â€”(C_nH_2n)â€”R^b(R^bis as defined above).

R^bis preferably

- (g) â€”NR^b5C(O)NR^b6R^b7(R^b5, R^b6and R^b7are as defined above),
- (h) â€”NR^b8R^b9(R^b8and R^b9are as defined above),
- (i) â€”NR^b10S(O)₂R^b11(R^b10and R^b11are as defined above),
- (j) â€”NR^b12C(O)OR^b13(R^b12and R^b13are as defined above), or
- (k) â€”NR^b14C(O)R^b15(R^b14and R^b15are as defined above, more preferably
- (k) â€”NR^b14C(O)R^b15(R^b14and R^b15are as defined above)

n is preferably 1 or 2, more preferably 1.

R^b14is preferably hydrogen or methyl, more preferably hydrogen.

R^b15is preferably

- (ii) C_1-4alkyl (said C_1-4alkyl is optionally substituted by 1 or 2 substituents selected from the group consisting of hydroxy, trifluoromethyl, C_1-4alkoxy and phenyl) or
- (iv) C_3-7cycloalkyl (said C_3-7cycloalkyl is optionally substituted by 1, 2, 3 or 4 substituents selected from the group consisting of C_1-4alkyl, halogen, hydroxyC_1-4alkyl and trifluoromethyl),
  more preferably C_1-4alkyl optionally substituted by 1 or 2 trifluoromethyls and C_1-4alkoxy, or C_3-7cycloalkyl optionally substituted by one trifluoromethyl, further preferably tert-butyl, 3,3,3-trifluoro-2,2-dimethylpropyl, 3,3,3-trifluoro-2-methoxy-2-methylpropyl, 3,3,3-trifluoro-2-methyl-2-trifluoromethylpropyl, or 1-trifluoromethylcyclopropyl.

R³is preferably

- (3) C_1-6alkyl or
- (4) â€”OR^c{R^cis C_1-6alkyl optionally substituted by 1, 2 or 3 substituents selected from the group consisting of the following (a) to (f);
- (a) halogen,
- (b) hydroxy,
- (c) C_1-6alkoxy,
- (d) â€”C(O)NR^c1R^c2(R^c1and R^c2are as defined above),
- (e) C_6-10aryl (said C_6-10aryl is optionally substituted by 1, 2 or 3 substituents selected from the group consisting of
  - (i) halogen,
  - (ii) hydroxy,
  - (iii) C_1-6alkyl,
  - (iv) C_1-6alkoxy, and
  - (v) haloC_1-4alkyl), and
- (f) 5- or 6-membered heteroaryl containing 1, 2 or 3 nitrogen atoms, oxygen atoms or sulfur atoms (said heteroaryl is optionally substituted by 1, 2 or 3 substituents selected from the group consisting of
  - (i) halogen,
  - (ii) hydroxy,
  - (iii) C_1-6alkyl,
  - (iv) C_1-6alkoxy, and
  - (v) haloC_1-4alkyl)}.

R^cis preferably methyl optionally substituted by 1 or 2 substituents selected from the following (e) and (f);

- (e) C_6-10aryl (said C_6-10aryl is optionally substituted by 1, 2 or 3 substituents selected from the group consisting of
  - (i) halogen,
  - (ii) hydroxy,
  - (iii) C_1-6alkyl,
  - (iv) C_1-6alkoxy, and
  - (v) haloC_1-4alkyl), and
- (f) 5- or 6-membered heteroaryl containing 1, 2 or 3 nitrogen atoms, oxygen atoms or sulfur atoms (said heteroaryl is optionally substituted by 1, 2 or 3 substituents selected from the group consisting of
  - (i) halogen,
  - (ii) hydroxy,
  - (iii) C_1-6alkyl,
  - (iv) C_1-6alkoxy, and
  - (v) haloC_1-4alkyl), more preferably methyl optionally substituted by 1 or 2 substituents selected from the following (e1) and (f1);
- (e1) phenyl (said phenyl is optionally substituted by 1 or 2 substituents selected from the group consisting of
  - (i) halogen,
  - (ii) hydroxy,
  - (iii) C_1-6alkyl,
  - (iv) C_1-6alkoxy, and
  - (v) haloC_1-4alkyl), and
- (f1) pyridyl (said pyridyl is optionally substituted by 1 or 2 substituents selected from the group consisting of
  - (i) halogen,
  - (ii) hydroxy,
  - (iii) C_1-6alkyl,
  - (iv) C_1-6alkoxy, and
  - (v) haloC_1-4alkyl)

R⁴is preferably hydrogen, fluoro, chloro, or methyl, more preferably hydrogen.

R⁵is preferably

- (1) halogen,
- (4) C_1-6alkyl (said C_1-6alkyl is optionally substituted by 1, 2 or 3 substituents selected from the group consisting of halogen, C_6-10aryl and C_1-6alkoxy),
- (5) C_3-7cycloalkyl,
- (6) â€”OR^d{R^dis
  - (a) C_2-6alkynyl,
  - (b) C_3-7cycloalkyl optionally substituted by 1, 2 or 3 C_1-6alkyls or
  - (c) C_1-8alkyl (said C_1-8alkyl is optionally substituted by 1, 2 or 3 substituents selected from the group consisting of the following (i) to (v);
    - (i) halogen,
    - (ii) C_6-10aryl,
    - (iii) C_1-6alkoxy,
    - (iv) C_3-7cycloalkyl (said C_3-7cycloalkyl is optionally substituted by 1, 2 or 3 substituents selected from the group consisting of C_1-6alkyl and haloC_1-4alkyl), and
    - (v) 4-, 5- or 6-membered saturated heterocyclyl containing one oxygen atom (said saturated heterocyclyl is optionally substituted by 1, 2 or 3 substituents selected from the group consisting of C_1-6alkyl and haloC_1-4alkyl))}, or
- (7) the formula:

[Image Omitted]

wherein R^eis

- (a) C_1-6alkyl,
- (b) C_3-7cycloalkyl,
- (c) 5- or 6-membered heteroaryl containing 1, 2 or 3 nitrogen atoms, oxygen atoms or sulfur atoms, or
- (d) C_6-10aryl (said C_6-10aryl is optionally substituted by 1, 2 or 3 substituents selected from the group consisting of
  - (i) halogen,
  - (ii) C_1-6alkyl,
  - (iii) haloC_1-4alkyl,
  - (iv) C_1-6alkoxy, and
  - (v) haloC_1-4alkoxy).

R^dis preferably C_1-8alkyl (said C_1-8alkyl is optionally substituted by 1, 2 or 3 substituents selected from the group consisting of the following (i) to (v);

- (i) halogen,
- (ii) C_6-10aryl,
- (iii) C_1-6alkoxy,
- (iv) C_3-7cycloalkyl (said C_3-7cycloalkyl is optionally substituted by 1, 2 or 3 substituents selected from the group consisting of C_1-6alkyl and haloC_1-4alkyl), and
- (v) 4-, 5- or 6-membered saturated heterocyclyl containing one oxygen atom (said saturated heterocyclyl is optionally substituted by 1, 2 or 3 substituents selected from the group consisting of C_1-6alkyl and haloC_1-4alkyl)).

R^eis preferably

- (b) C_3-7cycloalkyl,
- (c) 5- or 6-membered heteroaryl containing 1, 2 or 3 nitrogen atoms, oxygen atoms or sulfur atoms, or
- (d) C_6-10aryl (said C_6-10aryl is optionally substituted by 1, 2 or 3 substituents selected from the group consisting of
  - (i) halogen,
  - (ii) C_1-6alkyl,
  - (iii) haloC_1-4alkyl,
  - (iv) C_1-6alkoxy, and
  - (v) haloC_1-4alkoxy)

m1 is preferably 0, 1 or 2, more preferably 1 or 2.

In the compound represented by the formula [I], one of preferable embodiments is a compound represented by the following formula [I-A]:

[Image Omitted]

wherein
a carbon atom with a hydrogen atom is not substituted by R⁴and R⁵,
X, R¹, R²and R⁴are as defined in the aforementioned formula [I],

R⁵is

- (1) halogen,
- (4) C_1-6alkyl (said C_1-6alkyl is optionally substituted by 1, 2 or 3 substituents selected from the group consisting of halogen, C_6-10aryl and C_1-6alkoxy),
- (5) C_3-7cycloalkyl, or
- (6) â€”OR^d{R^dis
  - (a) C_2-6alkynyl or
  - (c) C_1-8alkyl (said C_1-8alkyl is optionally substituted by 1, 2 or 3 substituents selected from the group consisting of the following (i) to (v);
    - (i) halogen,
    - (ii) C_6-10aryl,
    - (iii) C_1-6alkoxy,
    - (iv) C_3-7cycloalkyl (said C_3-7cycloalkyl is optionally substituted by 1, 2 or 3 substituents selected from the group consisting of C_1-6alkyl and haloC_1-4alkyl), and
    - (v) 4-, 5- or 6-membered saturated heterocyclyl containing 1, 2 or 3 nitrogen atoms, oxygen atoms or sulfur atoms (said saturated heterocyclyl is optionally substituted by 1, 2 or 3 substituents selected from the group consisting of C_1-6alkyl and haloC_1-4alkyl))}, and m7 is 0, 1 or 2 and, when m7 is 2, each R⁵is selected independently.

In a compound represented by the formula [I], one of the preferable other embodiments is a compound represented by the following formula [I-B]:

[Image Omitted]

wherein
a carbon atom with a hydrogen atom is not substituted by R⁴and R⁵,
X, R³and R⁴are as defined in the aforementioned formula [I], R¹is chloro or trifluoromethyl,

R⁵is

- (4) C_1-6alkyl (said C_1-6alkyl is optionally substituted by 1, 2 or 3 substituents selected from the group consisting of halogen, C_6-10aryl and C_1-6alkoxy),
- (6) â€”OR^d{R^dis
  C_1-8alkyl (said C_1-8alkyl is optionally substituted by 1, 2 or 3 substituents selected from the group consisting of the following (i) to (iv);
- (i) halogen,
- (ii) C_6-10aryl,
- (iii) C_1-6alkoxy, and
- (iv) C_3-7cycloalkyl (said C_3-7cycloalkyl is optionally substituted by 1, 2 or 3 substituents selected from the group consisting of C_1-6alkyl and haloC_1-4alkyl))}, or
- (7) the formula:

[Image Omitted]

wherein R^eis

- (b) C_3-7cycloalkyl, or
- (d) C_6-10aryl (said C_6-10aryl is optionally substituted by 1, 2 or 3 substituents selected from the group consisting of
  - (i) halogen,
  - (ii) C_1-6alkyl,
  - (iii) haloC_1-4alkyl,
  - (iv) C_1-6alkoxy, and
  - (v) haloC_1-4alkoxy), and
    m7 is 0, 1 or 2 and, when m7 is 2, each R⁵is selected independently.

In a compound represented by the formula [I], one of the preferable other embodiments is a compound represented by the following formula [I-C]:

[Image Omitted]

wherein

X is CH or N,R^b15is

- (ii) C_1-4alkyl (said C_1-4alkyl is optionally substituted by 1 or 2 substituents selected from trifluoromethyl and methoxy) or
- (iv) C_3-7cycloalkyl optionally substituted by trifluoromethyl,

R^5ais

- (1) fluoro,
- (4) methyl (said methyl is optionally substituted by 3 fluoros), or
- (6) â€”OR^d{R^dis
  - (a) C_2-4alkynyl or
  - (c) C_1-4alkyl optionally substituted by one C_3-7cycloalkyl (said C_3-7cycloalkyl is optionally substituted by trifluoromethyl)},

R^5bis

- (1) halogen,
- (4) C_1-4alkyl, or
- (5) cyclopropyl, and
  m8 is 0 or 1.

A pharmaceutically acceptable salt of a compound represented by the formula [I](hereinafter to be also referred to as the compound of the present invention) may be any salt as long as it forms a nontoxic salt with the compound of the present invention, and examples thereof include salts with inorganic acid, salts with organic acid, salts with inorganic base, salts with organic base, salts with amino acid and the like.

Various forms of pharmaceutically acceptable salts are well known in this field and, for example, they are described in the following documents.

(a) Berge et al., J. Pharm. Sci., 66, p 1-19 (1977),
(b) Stahl et al., â€œHandbook of Pharmaceutical Salt: Properties, Selection, and Useâ€ (Wiley-VCH, Weinheim, Germany, 2002),
(c) Paulekuhn et al., J. Med. Chem., 50, p 6665-6672 (2007)

Examples of the salts with inorganic acid include salts with hydrochloric acid, nitric acid, sulfuric acid, phosphoric acid, hydrobromic acid and the like.

Examples of the salts with organic acid include salts with oxalic acid, maleic acid, citric acid, fumaric acid, lactic acid, malic acid, succinic acid, tartaric acid, acetic acid, trifluoroacetic acid, gluconic acid, ascorbic acid, methanesulfonic acid, benzenesulfonic acid, p-toluenesulfonic acid and the like.

Examples of the salts with organic acid include salts with adipic acid, alginic acid, 4-aminosalicylic acid, anhydromethylenecitric acid, benzoic acid, calcium edetate, camphoric acid, camphor-10-sulfonic acid, carbonic acid, edetic acid, ethane-1,2-disulfonic acid, dodecylsulfuric acid, ethanesulfonic acid, glucoheptonic acid, glucuronic acid, glucoheptonic acid, glycollyarsanilic acid, hexylresorcinic acid, hydrofluoric acid, hydroiodic acid, hydroxy-naphtoic acid, 2-hydroxy-1-ethanesulfonic acid, lactobionic acid, mandelic acid, methylsulfuric acid, methylnitric acid, methylenebis(salicylic acid), galactaric acid, naphthalene-2-sulfonic acid, 2-naphtoic acid, 1,5-naphthalenedisulfonic acid, oleic acid, pamoic acid, pantothenic acid, pectin acid, picric acid, propionic acid, polygalacturonic acid, salicylic acid, stearic acid, tannic acid, teoclic acid, thiocyanic acid, undecanoic acid and the like.

Examples of the salts with inorganic base include sodium salt, potassium salt, calcium salt, magnesium salt, ammonium salt and the like.

Furthermore, examples of the salts with inorganic base include salts with aluminum, barium, bismuth, lithium, or zinc.

Examples of the salts with organic base include salts with methylamine, diethylamine, trimethylamine, triethylamine, ethanolamine, diethanolamine, triethanolamine, ethylenediamine, tris(hydroxymethyl)methylamine, dicyclohexylamine, N,Nâ€²-dibenzylethylenediamine, guanidine, pyridine, picoline, choline, cinchonine, meglumine and the like.

Furthermore, examples of the salts with organic base include salts with arecoline, betaine, clemizole, N-methylglucamine, N-benzylphenethylamine or tris(hydroxymethyl)methylamine.

Examples of the salts with amino acid include salts with lysine, arginine, aspartic acid, glutamic acid and the like.

Among the above-mentioned salts, preferred are salts with hydrochloric acid, sulfuric acid or p-toluenesulfonic acid.

Various salts can be obtained by reacting a compound represented by the formula [I] with inorganic base, organic base, inorganic acid, organic acid or amino acid according to a known method.

A compound represented by the formula [I] or a pharmaceutically acceptable salt thereof may be present as a solvate. The â€œsolvateâ€ is a compound represented by the formula [I] or a pharmaceutically acceptable salt thereof, which is coordinated with a solvent molecule, and also encompasses hydrates. The solvate is preferably a pharmaceutically acceptable solvate, examples thereof include a hydrate, ethanolate, dimethyl sulfoxidate and the like of a compound represented by the formula [I] or a pharmaceutically acceptable salt thereof. Specific examples include semihydrate, monohydrate, dihydrate or monoethanolate of a compound represented by the formula [I], monohydrate of sodium salt or 2/3 ethanolate of dihydrochloride of a compound represented by the formula [I], and the like.

The solvates can be obtained by a known method.

In addition, a compound represented by the formula [I] may be labeled with isotope (e.g., ²H, ³H, ¹⁴C, ³⁵S etc.)

The compound of the present invention may exist as a tautomer. In this case, the compound of the present invention can be a single tautomer or a mixture of individual tautomers. For example, a compound represented by the formula [I] may contain a tautomer shown below

[Image Omitted]

Such tautomer is also encompassed in the compound represented by the formula [I].

The compound of the present invention may have a carbon double bond. In this case, the compound of the present invention can be present as E form, Z form, or a mixture of E form and Z form.

The compound of the present invention may contain a stereoisomer that should be recognized as a cis/trans isomer. In this case, the compound of the present invention can be present as a cis form, a trans form, or mixture of a cis form and a trans form.

The compound of the present invention may contain one or more asymmetric carbons. In this case, the compound of the present invention may be present as a single enantiomer, a single diastereomer, a mixture of enantiomers or a mixture of diastereomers.

The compound of the present invention may be present as an atropisomer. In this case, the compound of the present invention may be present as an individual atropisomer or a mixture of atropisomers.

The compound of the present invention may simultaneously contain plural structural characteristics that produce the above-mentioned isomers. Moreover, the compound of the present invention may contain the above-mentioned isomers at any ratio.

In the absence of other reference such as annotation and the like, the formulae, chemical structures and compound names indicated in the present specification without specifying the stereochemistry thereof encompass all the above-mentioned isomers that may exist.

A diastereomeric mixture can be separated into each diastereomer by conventional methods such as chromatography, crystallization and the like. In addition, each diastereomer can also be formed by using a stereochemically single starting material, or by a synthesis method using a stereoselective reaction.

An enantiomeric mixture can be separated into each single enantiomer by a method well known in the pertinent field.

For example, enantiomeric mixture can be prepared by reacting the enantiomeric mixture with a substantially pure enantiomer that is known as a chiral auxiliary. The diastereomeric mixture can be separated into each diastereomer mentioned above. The diastereomer mixture can be separated into each diastereomer as mentioned above. The separated diastereomer can be converted to a desired enantiomer by removing the added chiral auxiliary by cleavage.

In addition, a mixture of enantiomers of a compound can also be directly separated by a chromatography method using a chiral solid phase well known in the pertinent field.

Alternatively, one of the enantiomers of a compound can also be obtained by using a substantially pure optically active starting material or stereoselective synthesis (asymmetric induction) of a prochiral intermediate using a chiral auxiliary and an asymmetric catalyst.

The absolute steric configuration can be determined based on the X-ray crystal analysis of the resultant crystalline product or intermediate. In this case, a resultant crystalline product or intermediate derivatized with a reagent having an asymmetric center with a known steric configuration may be used where necessary.

As a compound represented by the formula [I] or a pharmaceutically acceptable salt thereof, a substantially purified compound represented by the formula [I] or a pharmaceutically acceptable salt thereof is preferable. More preferred is a compound represented by the formula [I] or a pharmaceutically acceptable salt thereof or a solvate thereof, which is purified to have a purity of more than 80%.

Examples of the â€œpharmaceutical compositionâ€ include oral preparations such as tablet, capsule, granule, powder, troche, syrup, emulsion, suspension and the like, and parenteral agents such as external preparation, suppository, injection, eye drop, nasal preparations, pulmonary preparation and the like.

The pharmaceutical composition of the present invention is produced according to a method known per se in the art of pharmaceutical preparations, by mixing etc. a compound represented by the formula [I] or a pharmaceutically acceptable salt thereof, or a solvate thereof with a suitable amount of at least one kind of pharmaceutically acceptable carrier and the like as appropriate. While the content of the compound represented by the formula [I] or a pharmaceutically acceptable salt thereof, or a solvate thereof in the pharmaceutical composition varies depending on the dosage form, dose and the like, it is, for example, 0.00001 to 100 wt % of the whole composition.

Examples of the â€œpharmaceutically acceptable carrierâ€ include various organic or inorganic carrier substances conventionally used as preparation materials, for example, excipient, disintegrant, binder, glidant, lubricant and the like for solid preparations, and solvent, solubilizing agent, suspending agent, isotonicity agent, buffering agent, soothing agent, surfactant, pH adjuster, thickening agent and the like for liquid preparations. Where necessary, moreover, additives such as preservative, antioxidant, colorant, sweetening agent and the like are used.

Examples of the â€œexcipientâ€ include lactose, sucrose, D-mannitol, D-sorbitol, cornstarch, dextrin, microcrystalline cellulose, crystalline cellulose, carmellose, carmellose calcium, sodium carboxymethyl starch, low-substituted hydroxypropylcellulose, gum arabic and the like.

Examples of the â€œdisintegrantâ€ include carmellose, carmellose calcium, carmellose sodium, sodium carboxymethyl starch, croscarmellose sodium, crospovidone, low-substituted hydroxypropylcellulose, hydroxypropylmethylcellulose, crystalline cellulose and the like.

Examples of the â€œbinderâ€ include hydroxypropylcellulose, hydroxypropylmethylcellulose, povidone, crystalline cellulose, sucrose, dextrin, starch, gelatin, carmellose sodium, gum arabic and the like.

Examples of the â€œglidantâ€ include light anhydrous silicic acid, magnesium stearate and the like.

Examples of the â€œlubricantâ€ include magnesium stearate, calcium stearate, talc and the like.

Examples of the â€œsolventâ€ include purified water, ethanol, propylene glycol, macrogol, sesame oil, corn oil, olive oil and the like.

Examples of the â€œsolubilizing agentâ€ include propylene glycol, D-mannitol, benzyl benzoate, ethanol, triethanolamine, sodium carbonate, sodium citrate and the like.

Examples of the â€œsuspending agentâ€ include benzalkonium chloride, carmellose, hydroxypropylcellulose, propylene glycol, povidone, methylcellulose, glycerol monostearate and the like.

Examples of the â€œisotonicity agentâ€ include glucose, D-sorbitol, sodium chloride, D-mannitol and the like.

Examples of the â€œbuffering agentâ€ include sodium hydrogenphosphate, sodium acetate, sodium carbonate, sodium citrate and the like.

Examples of the â€œsoothing agentâ€ include benzyl alcohol and the like.

Examples of the â€œsurfactantâ€ include polyoxyethylene hydrogenated castor oil, polyethylene glycol monostearate, polyoxyethylene sorbitan fatty acid ester, alkyldiaminoethylglycine, alkylbenzenesulfonate, benzethonium chloride and the like.

Examples of the â€œpH adjusterâ€ include hydrochloric acid, sulfuric acid, phosphoric acid, citric acid, acetic acid, sodium hydrogen carbonate, sodium carbonate, potassium hydroxide, sodium hydroxide, monoethanolamine, triethanolamine and the like.

Examples of the â€œthickening agentâ€ include polyvinyl alcohol, carboxyvinyl polymer, methylcellulose, hydroxyethylcellulose, polyethylene glycol, dextran and the like.

Examples of the â€œpreservativeâ€ include ethyl parahydroxybenzoate, chlorobutanol, benzyl alcohol, sodium dehydroacetate, sorbic acid and the like.

Examples of the â€œantioxidantâ€ include sodium sulfite, ascorbic acid and the like.

Examples of the â€œcolorantâ€ include food colors (e.g., Food Color Red No. 2 or 3, Food Color Yellow No. 4 or 5 etc.), 3-carotene and the like.

Examples of the â€œsweetening agentâ€ include saccharin sodium, dipotassium glycyrrhizinate, aspartame and the like.

The pharmaceutical composition of the present invention can be administered orally or parenterally (e.g., topical, rectal, intravenous administration etc.) to human as well as mammals other than human (e.g., hamster, guinea pig, cat, dog, swine, bovine, horse, sheep, monkey etc.). The dose varies depending on the subject of administration, disease, symptom, dosage form, administration route and the like. For example, the daily dose for oral administration to an adult patient (body weight: about 60 kg) is generally within the range of about 0.1 g to 10 g, based on the compound of the present invention as the active ingredient. This amount can be administered in one to several portions.

The above-mentioned compound represented by the formula [I] or a pharmaceutically acceptable salt thereof, or a solvate thereof can be used in combination with one or a plurality of other medicaments (hereinafter to be also referred to as a concomitant drug) according to a method generally employed in the medical field (hereinafter to be referred to as combined use).

The administration period of the above-mentioned compound represented by the formula [I] or a pharmaceutically acceptable salt thereof, and a concomitant drug is not limited, and they may be administered to an administration subject as combination preparation, or the both preparations may be administered simultaneously or at given intervals as individual preparations. In addition, the pharmaceutical composition of the present invention and a concomitant drug may be used in the form of a kit. The dose of the concomitant drug is similar to the clinically-employed dose and can be appropriately selected according to the subject of administration, disease, symptom, dosage form, administration route, administration time, combination and the like. The administration form of the concomitant drug is not particularly limited, and it is only required that the compound represented by the formula [I] or a pharmaceutically acceptable salt thereof, or a solvate thereof is combined with a concomitant drug.

Examples of the concomitant drug include therapeutic agents for glaucoma such as prostaglandin preparation, Î² blocker, Î± receptor agonist, sympathetic nerve stimulation agent, Î± blocker, carbonic anhydrase inhibitor, anticholinesterase agent, Rho kinase inhibitor and the like.

Examples of the prostaglandin preparation include isopropyl unoprostone, latanoprost, travoprost, tafluprost, bimatoprost and the like.

Examples of the Î² blocker include timolol maleate, Befunolol hydrochloride, carteolol hydrochloride, betaxolol hydrochloride, nipradilol, levobunolol hydrochloride and the like.

Examples of the Î± receptor agonist include brimonidine tartrate and the like.

Examples of the sympathetic nerve stimulation agent include dipivefrin hydrochloride, pilocarpine hydrochloride and the like.

Examples of the Î± blocker include bunazosin hydrochloride and the like.

Examples of the carbonic anhydrase inhibitor include dorzolamide hydrochloride, brinzolamide and the like.

Examples of the anticholinesterase agent include distigmine bromide and the like.

Examples of the Rho kinase inhibitor include ripasudil hydrochloride hydrate and the like.

An example of the specific combination of medicaments is a combination of one medicament selected from latanoprost, travoprost, tafluprost, timolol maleate, dorzolamide hydrochloride and brinzolamide, and the above-mentioned compound represented by the formula [I] or a pharmaceutically acceptable salt thereof, or a solvate thereof.

Next, one example of the production methods of the compound to practice the present invention is explained below. However, the production method of the compound of the present invention or a pharmaceutically acceptable salt thereof is not limited thereto.

Even when no directly corresponding disclosure is found in the following Production Methods, the steps may be modified for efficient production of the compound, such as introduction of a protecting group into a functional group with deprotection in a subsequent step, changing the order of Production Methods and steps, appropriate use of reagents other than the exemplified reagents to promote progress of the reactions, and the like.

The treatment after reaction in each step may be conventional ones, where isolation and purification can be performed as necessary according to a method appropriately selected from conventional methods such as crystallization, recrystallization, distillation, partitioning, silica gel chromatography, preparative HPLC and the like, or a combination of those methods. In some cases, the next step may be conducted without isolation and purification.

An intermediate capable of forming a salt may also be obtained as a salt, or used as a salt for reactions. Examples of such salt include hydrochloride of an intermediate having an amino group.

[Production Method 1-1]

[Image Omitted]

wherein Hal¹is chloro or bromo;
R⁶is C_1-6alkyl such as methyl, ethyl and the like or benzyl;
Z is a boron substituent used for the Suzuki coupling reaction such as â€”B(OH)₂, â€”B(OR⁷)₂(wherein R⁷is C_1-4alkyl or one R⁷may be bonded to the other R⁷to form a ring), â€”BF₃, the formula

[Image Omitted]

and the like; and
X, Cy, R⁵and m1 are as defined in the aforementioned formula [I].

(Step 1-1-1)

Compound [3] can be obtained by the Suzuki coupling reaction of compound [1] and compound [2]. For example, compound [3] can be obtained by reacting compound [1] with compound [2] under heating in a solvent in the presence of a base and a palladium catalyst. Where necessary, a ligand may be added. Not less than 1.5 equivalents of compound [1] are preferably used relative to compound [2] to prevent the Suzuki coupling reaction from progressing twice.

Examples of the palladium catalyst to be used for the reaction include palladium acetate, tetrakistriphenylphosphinepalladium, bis(triphenylphosphine)palladium dichloride, (bis(diphenylphosphino)ferrocene)palladium dichloride-methylene chloride complex and the like.

Examples of the base to be used for the reaction include inorganic bases such as alkali metal salts (e.g., potassium phosphate, sodium carbonate, sodium hydrogen carbonate, potassium carbonate, potassium acetate, sodium acetate, cesium fluoride etc.) and the like, and organic bases such as triethylamine and the like.

Examples of the ligand to be used for the reaction include organic phosphine ligands (e.g., triphenylphosphine, tricyclohexylphosphine, 2,2â€²-bis(diphenylphosphino)-1,1â€²-binaphthalene, 2-dicyclohexylphosphino-2â€²,6â€²-dimethoxybiphenyl etc.) and the like.

Examples of the solvent to be used for the reaction include ether solvents such as 1,4-dioxane, tetrahydrofuran, diethyl ether, 1,2-dimethoxyethane, cyclopentyl methyl ether and the like; alcohol solvents such as methanol, ethanol, 1-propanol, 2-propanol and the like; hydrocarbon solvents such as toluene, xylene, hexane and the like; polar solvents such as N,N-dimethylformamide, N,N-dimethylacetamide, dimethyl sulfoxide, acetonitrile and the like; a mixed solvent thereof, and a mixed solvent thereof with water.

Compound [1] may be a commercially available product such as 2,4-dichloro-6-methoxy-1,3,5-triazine, or may be obtained by converting a commercially available product as appropriate by a method well known to those of ordinary skill in the art.

As for the Suzuki coupling reaction, for example, the following review article is known (SUZUKI, A et al. Palladium-Catalyzed Cross-Coupling Reactions of Organoboron Compounds. Chem Rev. 1995, Vol. 95, pages 2457-2483).

(Step 1-1-2)

Compound [5] can be obtained by the Suzuki coupling reaction of compound [3] and compound [4]. For example, compound [5] can be obtained by reacting compound [3] with compound [4] under heating in a solvent in the presence of a base and a palladium catalyst. Where necessary, a ligand may be added.

Examples of the palladium catalyst to be used for the reaction include palladium acetate, tetrakistriphenylphosphinepalladium, bis(triphenylphosphine)palladium dichloride, (bis(diphenylphosphino)ferrocene)palladium dichloride-methylene chloride complex and the like.

Examples of the base to be used for the reaction include inorganic bases such as alkali metal salts (e.g., potassium phosphate, sodium carbonate, sodium hydrogen carbonate, potassium carbonate, potassium acetate, sodium acetate, cesium fluoride etc.) and the like, and organic bases such as triethylamine and the like.

Examples of the ligand to be used for the reaction include organic phosphine ligands such as triphenylphosphine, tricyclohexylphosphine, 2,2â€²-bis(diphenylphosphino)-1,1â€²-binaphthalene, 2-dicyclohexylphosphino-2â€²,6â€²-dimethoxybiphenyl and the like, and the like.

Examples of the solvent to be used for the reaction include ether solvents such as 1,4-dioxane, tetrahydrofuran, diethyl ether, 1,2-dimethoxyethane, cyclopentyl methyl ether and the like; alcohol solvents such as methanol, ethanol, 1-propanol, 2-propanol and the like; hydrocarbon solvents such as toluene, xylene, hexane and the like; polar solvents such as N,N-dimethylformamide, N,N-dimethylacetamide, dimethyl sulfoxide, acetonitrile and the like; a mixed solvent thereof, and a mixed solvent thereof with water.

(Step 1-1-3)

Compound [I] can be obtained by converting the alkoxy of compound [5] to hydroxy by hydrolysis. For example, when R⁶is C_1-6alkyl, compound [I] can be obtained by reacting compound [5] in a solvent in the presence of a base at room temperature to under heating, and neutralizing the obtained solution.

Examples of the base to be used for the reaction include lithium hydroxide, sodium hydroxide, potassium hydroxide, sodium methoxide and the like.

Examples of the solvent to be used for the reaction include a mixed solvent of water and alcohol solvents such as methanol, ethanol, 1-propanol, 2-propanol and the like; and a mixed solvent thereof with ether solvents such as 1,4-dioxane, tetrahydrofuran, diethyl ether, 1,2-dimethoxyethane, cyclopentyl methyl ether and the like.

[Production Method 1-2]

Compound [2] can be obtained by, for example, Production Method 1-2.

[Production Method 1-2]

[Image Omitted]

wherein L¹is a leaving group such as bromo, iodo, trifluoromethanesulfonyloxy and the like, X, R⁵and m1 are as defined in the aforementioned formula [I], and Z is as defined in the aforementioned Production Method 1-1.

(Step 1-2)

Compound [2] can be obtained by borating compound [6]. For example, compound [2] can be obtained by reacting compound [6] with a boron reagent under heating in the presence of a base and a palladium catalyst. Where necessary, a ligand may be added

Examples of the boron reagent to be used for the reaction include 4,4,4â€²,4â€²,5,5,5â€²,5â€²-octamethyl-2,2â€²-bi-1,3,2-dioxaborolane, 5,5,5â€²,5â€²-tetramethyl-2,2â€²-bi-1,3,2-dioxaborinane, tetrahydroxydiboron, 4,4,5,5-tetramethyl-1,3,2-dioxaborolane and the like.

Examples of the palladium catalyst to be used for the reaction include palladium acetate, tetrakistriphenylphosphinepalladium, bis(triphenylphosphine)palladium dichloride, (bis(diphenylphosphino)ferrocene)palladium dichloride-methylene chloride complex and the like.

Examples of the base to be used for the reaction include inorganic bases such as alkali metal salts (e.g., potassium phosphate, sodium carbonate, sodium hydrogen carbonate, potassium carbonate, potassium acetate, sodium acetate, cesium fluoride etc.) and the like, and organic bases such as triethylamine and the like.

Examples of the ligand to be used for the reaction include organic phosphorus ligands (e.g., triphenylphosphine, tricyclohexylphosphine, 2,2â€²-bis(diphenylphosphino)-1,1â€²-binaphthalene, 2-dicyclohexylphosphino-2â€²,6â€²-dimethoxybiphenyl etc.) and the like.

Examples of the solvent to be used for the reaction include ether solvents such as 1,4-dioxane, tetrahydrofuran, diethyl ether, 1,2-dimethoxyethane, cyclopentyl methyl ether and the like; alcohol solvents such as methanol, ethanol, 1-propanol, 2-propanol and the like; hydrocarbon solvents such as toluene, xylene, hexane and the like; polar solvents such as N,N-dimethylformamide, N,N-dimethylacetamide, dimethyl sulfoxide, acetonitrile and the like; a mixed solvent thereof, and a mixed solvent thereof with water.

Compound [2] can also be obtained by adding an organic metal reagent to compound [6] in a solvent at âˆ’78Â° C. to room temperature, and reacting the product with a boron compound at âˆ’78Â° C. to room temperature.

Examples of the organic metal reagent to be used for the reaction include n-butyllithium, tert-butyllithium, isopropylmagnesium chloride and the like.

Examples of the boron reagent to be used for the reaction include trimethyl borate, triisopropyl borate, 2-isopropoxy-4,4,5,5-tetramethyl-1,3,2-dioxaborolane and the like.

Examples of the solvent to be used for the reaction include ether solvents such as 1,4-dioxane, tetrahydrofuran, diethyl ether, 1,2-dimethoxyethane, cyclopentyl methyl ether and the like; hydrocarbon solvents such as toluene, xylene, hexane and the like, and a mixed solvent thereof.

In one embodiment, compound [6] may be a commercially available product such as those shown below, or may be obtained by converting a commercially available product as appropriate by a method well known to those of ordinary skill in the art.

[Image Omitted]

In one embodiment, compound [2] may be a commercially available product such as those shown below, or may be obtained by converting a commercially available product as appropriate by a method well known to those of ordinary skill in the art.

[Image Omitted]

wherein Z is as defined in the aforementioned Production Method 1-1.

[Production Method 1-3]

Compound [4] can be obtained by, for example, Production Method 1-3.

[Production Method 1-3]

[Image Omitted]

wherein R¹, R², R³and R⁴are as defined in the aforementioned formula [I], L¹is as defined in the aforementioned Production Method 1-2, and Z is as defined in the aforementioned Production Method 1-1.

(Step 1-3)

Compound [4] is compound [8a] or [8b]. Compound [8a] or [8b], i.e., compound [4], can be obtained by borating compound [7a] or [7b] in the same manner as in Production Method 1-2, Step 1-2.

Compounds [7a] and [7b] may be commercially available products such as 2-bromo-4-methylbenzonitrile and 2-bromo-3-methylphenol, or may be obtained by converting a commercially available product as appropriate by a method well known to those of ordinary skill in the art.

In one embodiment, compound [4] may be a commercially available product such as those shown below, or may be obtained by converting a commercially available product as appropriate by a method well known to those of ordinary skill in the art.

[Image Omitted]

wherein Z is as defined in the aforementioned Production Method 1-1.

[Production Method 2-1] or [Production Method 2-3]

For example, compound [I-a1] which is a compound represented by the formula [I] wherein ring Cy is the formula

[Image Omitted]

wherein R¹, R⁴, R^b15and n are as defined in the aforementioned formula [I], can be obtained by appropriately converting the substituent of ring Cy.

When C_nH_2nis a straight chain, Production Method 2-1 is preferable, and when C_nH_2nis a branched chain, Production Method 2-3 is preferable.

[Production Method 2-1]

[Image Omitted]

wherein Y is the formula

[Image Omitted]

wherein R⁵, R⁶and m1 are as defined in the aforementioned formula [I];
C_1-6Alkyl is C_1-6alkyl;
t is 0, 1, 2 or 3, â€”(C_tH_2t)â€” may be a straight or branched chain;
Hal²is bromo or iodo;
P^vis a hydroxy-protecting group such as methoxymethyl and the like;
P^wis an amino-protecting group such as tert-butoxycarbonyl and the like;
L²is a leaving group such as halogen (e.g., chloro, bromo and the like), methanesulfonyloxy, p-toluenesulfonyloxy and the like;
R¹, R⁴, R⁶, R^b15and n are as defined in the aforementioned formula [I], and Z is as defined in the aforementioned Production Method 1-1.

(Step 2-1-1)

Compound [10] can be obtained by converting the ester of compound [9] to carboxy by hydrolysis. For example, compound [10] can be obtained by reacting compound [9] in a solvent in the presence of a base at room temperature to under heating, and neutralizing the obtained solution.

Examples of the base to be used for the reaction include lithium hydroxide, sodium hydroxide, potassium hydroxide, sodium methoxide and the like.

Examples of the solvent to be used for the reaction include a mixed solvent of water and alcohol solvents such as methanol, ethanol, 1-propanol, 2-propanol and the like; and a mixed solvent thereof with ether solvents such as 1,4-dioxane, tetrahydrofuran, diethyl ether, 1,2-dimethoxyethane, cyclopentyl methyl ether and the like.

Compound [9] may be a commercially available product such as those shown below, or may be obtained by converting a commercially available product as appropriate by a method well known to those of ordinary skill in the art.

[Image Omitted]

(Step 2-1-2)

Compound [11] can be obtained by converting the carboxy of compound [10] to hydroxy by reduction. For example, compound [11] can be obtained by reacting compound [10] with a reducing agent in a solvent under ice-cooling to room temperature.

Examples of the reducing agent to be used for the reaction include lithium aluminum hydride, diisobutylaluminum hydride, sodium bis(2-methoxyethoxy)aluminum hydride, borane-tetrahydrofuran complex and the like.

Examples of the solvent to be used for the reaction include tetrahydrofuran, diethyl ether, 1,2-dimethoxyethane, cyclopentyl methyl ether, toluene, xylene, hexane and the like and a mixed solvent thereof.

(Step 2-1-3)

Compound [12] can be obtained by protecting the hydroxy group of compound [11]. The protection reaction can be performed by a known method according to the protecting group to be employed.

For example, when P^vis methoxymethyl, compound [12] can be obtained by reacting compound [11] with chloromethyl methyl ether in a solvent such as tetrahydrofuran, 1,2-dimethoxyethane, cyclopentyl methyl ether, N,N-dimethylformamide and the like in the presence of a base such as sodium hydride and the like from ice-cooling to room temperature.

(Step 2-1-4)

Compound [13] can be obtained by borating compound [12] in the same manner as in Production Method 1-2, Step 1-2.

(Step 2-1-5)

Compound [14] can be obtained by the Suzuki coupling reaction of compound [3] and compound [13] in the same manner as in Production Method 1-1, Step 1-1-2.

(Step 2-1-6)

Compound [15] can be obtained by removing P^vof compound [14] by hydroxy-deprotection by a conventional method. The deprotection reaction can be performed by a known method according to the protecting group to be employed.

For example, when P^vis methoxymethyl, a treatment with an acid such as hydrochloric acid, trifluoroacetic acid, methanesulfonic acid and the like only needs to be performed in a single or mixed solvent of chloroform, 1,4-dioxane, tetrahydrofuran, diethyl ether, 1,2-dimethoxyethane, cyclopentyl methyl ether, ethyl acetate, ethanol, methanol, water and the like.

Compound [15] can also be obtained by the Suzuki coupling reaction of compound [3] and compound [23] represented by the formula

[Image Omitted]

wherein R¹and R⁴are as defined in the aforementioned formula [I], Z is as defined in the aforementioned Production Method 1-1, and t is as defined in the aforementioned Production Method 2-1,
in the same manner as in Production Method 1-1, Step 1-1-2.

(Step 2-1-7)

Compound [16] can be obtained by converting the hydroxy of compound [15] to the leaving group L². For example, when L²is methanesulfonyloxy, compound [16] can be obtained by reacting compound [15] with methanesulfonyl chloride in a solvent in the presence of a base at room temperature. When L²is bromo, compound [16] can be obtained by reacting compound [15] with carbon tetrabromide in a solvent in the presence of triphenylphosphine from ice-cooling to room temperature.

Examples of the base to be used for the reaction include triethylamine, pyridine and the like.

Examples of the solvent to be used for the reaction include ether solvents such as 1,4-dioxane, tetrahydrofuran, diethyl ether, 1,2-dimethoxyethane, cyclopentyl methyl ether and the like; hydrocarbon solvents such as toluene, hexane, xylene and the like; halogenated solvents such as dichloromethane, chloroform and the like; and polar solvents such as N,N-dimethylformamide, N,N-dimethylacetamide, dimethyl sulfoxide, acetonitrile and the like.

It is possible to use dimethylsulfide instead of the above-mentioned triphenylphosphine, and N-bromosuccinimide can be used instead of the above-mentioned carbon tetrabromide.

p-Toluenesulfonyl chloride and benzenesulfonyl chloride can be used instead of the above-mentioned methanesulfonyl chloride.

(Step 2-1-8)

Compound [18] can be obtained by reacting compound [16] in a solvent in the presence of a base at room temperature to under heating compound [17]. Examples of the protecting group P^winclude tert-butoxycarbonyl.

Examples of the base to be used for the reaction include inorganic bases such as alkali metal salts (e.g., cesium carbonate, potassium phosphate, sodium carbonate, potassium carbonate etc.) and the like.

Examples of the solvent to be used for the reaction include polar solvents such as N,N-dimethylformamide, N,N-dimethylacetamide, dimethyl sulfoxide, acetonitrile and the like.

(Step 2-1-9)

Compound [19] can be obtained by removing P^wof compound [18] by amine-deprotection by a conventional method. The deprotection reaction can be performed by a known method according to the protecting group to be employed.

For example, when P^wis tert-butoxycarbonyl, a treatment with an acid such as hydrochloric acid, trifluoroacetic acid, methanesulfonic acid and the like only needs to be performed in a solvent.

Examples of the solvent to be used for the reaction include ether solvents such as 1,4-dioxane, tetrahydrofuran, diethyl ether, 1,2-dimethoxyethane, cyclopentyl methyl ether and the like; hydrocarbon solvents such as toluene, hexane, xylene and the like; halogenated solvents such as dichloromethane, chloroform and the like; ester solvents such as ethyl acetate and the like; and alcohol solvents such as methanol, ethanol, 1-propanol, 2-propanol and the like.

(Step 2-1-10)

Compound [21] can be obtained by a conventional amide bond forming reaction, for example, by reacting compound [19] with compound [20] in a solvent in the presence of a condensing agent and an additive. A base may be added as necessary.

Examples of the condensing agent to be used for the reaction include dicyclohexylcarbodiimide (DCC), 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (WSC.HCl), diisopropylcarbodiimide, 1,1â€²-carbonyldiimidazole (CDI), O-(7-azabenzotriazol-1-yl)-N,N,Nâ€²,Nâ€²-tetramethyluronium hexafluorophosphate (HATU), (benzotriazol-1-yloxy)tris(pyrrolidino)phosphonium hexafluorophosphate (PyBOP), diphenylphosphoryl azide and the like.

Examples of the additive to be used for the reaction include 1-hydroxybenzotriazole (HOBt), 1-hydroxy-7-azabenzotriazole (HOAt), N-hydroxysuccinimide (HOSu), 4-dimethylaminopyridine and the like.

Examples of the base to be used for the reaction include organic bases such as pyridine, triethylamine and the like.

Examples of the solvent to be used for the reaction include ether solvents such as 1,4-dioxane, tetrahydrofuran, diethyl ether, 1,2-dimethoxyethane, cyclopentyl methyl ether and the like; hydrocarbon solvents such as toluene, hexane, xylene and the like; halogenated solvents such as dichloromethane, chloroform and the like; and polar solvents such as N,N-dimethylformamide, N,N-dimethylacetamide, dimethyl sulfoxide, acetonitrile, pyridine and the like. These may be used singly or as a mixture of two or more kinds thereof.

Compound [20] may be a commercially available product such as cyclopentanecarboxylic acid and 1-(trifluoromethyl)cyclopropane-1-carboxylic acid, or may be obtained by converting a commercially available product as appropriate by a method well known to those of ordinary skill in the art.

(Step 2-1-11)

Compound [21] can be indicated as compound [22]. Compound [I-a1] can be obtained by converting the alkoxy of compound [22] to hydroxy by hydrolysis in the same manner as in Production Method 1-1, Step 1-1-3.

[Production Method 2-2]

Compound [10a] which is compound [10] wherein R¹is C_1-6alkyl or chloro can be obtained by [Production Method 2-2].

[Production Method 2-2]

[Image Omitted]

wherein R^xis C_1-6alkyl or chloro;
R⁴is as defined in the aforementioned formula [I], and Hal²and t are as defined in the aforementioned Production Method 2-1.

(Step 2-2)

Compound [10a] can be obtained by halogenating compound [24]. For example, when Hal²is iodo, compound [10a] can be obtained by reacting compound [24] with N-iodosuccinimide in an acid at room temperature.

Examples of the acid to be used for the reaction include concentrated sulfuric acid and the like.

Compound [24] may be a commercially available product such as 4-chlorophenylacetic acid, 3-(4-chlorophenyl)propionic acid, 4-(4-chlorophenyl)butanoic acid, 4-methylphenylacetic acid and 2-(4-methylphenyl)propionic acid, or may be obtained by converting a commercially available product as appropriate by a method well known to those of ordinary skill in the art.

[Production Method 2-3]

[Image Omitted]

wherein j and k are each 0, 1, 2 or 3, j+k=nâˆ’1;
R¹, R⁴, R⁵, R^b15and n are as defined in the aforementioned formula [I],
Z is as defined in the aforementioned Production Method 1-1, and
Hal², Y, P^wand L²are as defined in the aforementioned Production Method 2-1.

(Step 2-3-1)

Compound [26] can be obtained by borating compound [25] in the same manner as in Production Method 1-2, Step 1-2.

Compound [25] may be a commercially available product such as 1-(3-bromo-4-chlorophenyl)propan-1-one and 1-(3-bromo-4-chlorophenyl)butan-1-one, or may be obtained by converting a commercially available product as appropriate by a method well known to those of ordinary skill in the art.

(Step 2-3-2)

Compound [27] can be obtained by the Suzuki coupling reaction of compound [3] and compound [26] in the same manner as in Production Method 1-1, Step 1-1-2.

(Step 2-3-3)

Compound [28] can be obtained by converting the carboxy of compound [27] to hydroxy by reduction. For example, compound [28] can be obtained by reacting compound [27] with a reducing agent in a solvent under ice-cooling to room temperature.

Examples of the reducing agent to be used for the reaction include sodium borohydride and the like.

Examples of the solvent to be used for the reaction include methanol, ethanol, 2-propanol, 1,4-dioxane, tetrahydrofuran, diethyl ether, 1,2-dimethoxyethane, cyclopentyl methyl ether and the like.

(Step 2-3-4)

Compound [29] can be obtained by converting the hydroxy of compound [28] to the leaving group L²in the same manner as in Production Method 2-1, Step 2-1-7.

(Step 2-3-5)

Compound [30] can be obtained by reacting compound [29] with compound [17] in the same manner as in Production Method 2-1, Step 2-1-8.

(Step 2-3-6)

Compound [31] can be obtained by removing P^wof compound [30] in the same manner as in Production Method 2-1, Step 2-1-9.

(Step 2-3-7)

Compound [32] can be obtained by reacting compound [31] with compound [20] in the same manner as in Production Method 2-1, Step 2-1-10.

(Step 2-3-8)

Compound [32] can be indicated as compound [22].

Compound [I-a1] can be obtained by converting alkoxy of compound [22] to hydroxy by hydrolysis in the same manner as in Production Method 1-1, Step 1-1-3.

In Production Method 2-1, compound [I-a2] which is a compound represented by the formula [I] wherein ring Cy is the formula

[Image Omitted]

wherein R¹, R⁴and n are as defined in the aforementioned formula [I], can be obtained by subjecting compound [9] to the reactions of Step 2-1-4, Step 2-1-5 and Step 2-1-11.

In Production Method 2-1, the amide bond forming reaction is performed by using compound [10] and HNR^b1R^b2such as dimethylamine, tert-butylamine and the like and in the same manner as in Step 2-1-10. Thereafter, the resultant product is subjected to the reactions of Step 2-1-4, Step 2-1-5 and Step 2-1-11, whereby compound [I-a3] which is a compound represented by the formula [I] wherein ring Cy is the formula

[Image Omitted]

wherein R¹, R⁴, R^b1, R^b2and n are as defined in the aforementioned formula [I], can be obtained.
In Production Method 2-1, compound [I-a4] which is a compound represented by the formula [I] wherein ring Cy is the formula

[Image Omitted]

wherein R¹, R⁴and n are as defined in the aforementioned formula [I] can be obtained by subjecting compound [15] to the reaction of Step 2-1-11.

In Production Method 2-1, the reaction of Step 2-1-11 is performed by using compound [15]. Thereafter, the resultant product is reacted with a C_1-6alkyl-carboxylic anhydride such as acetic anhydride, propionic anhydride and the like, whereby compound [I-a5] which is a compound represented by the formula [I] wherein ring Cy is the formula

[Image Omitted]

wherein R¹, R⁴and n are as defined in the aforementioned formula [I] and C_1-6Alkyl is as defined in the aforementioned Production Method 2-1, can be obtained.

In Production Method 2-1, compound [15] is reacted with ClC(O)NR^b3R^b4such as dimethylcarbamoyl chloride, diethylcarbamoyl chloride and the like in the presence of a base. Thereafter, the resultant product is subjected to the reaction of Step 2-1-11, whereby compound [I-a6] which is a compound represented by the formula [I] wherein ring Cy is the formula

[Image Omitted]

wherein R¹, R⁴, R^b, R^b4and n are as defined in the aforementioned formula [I], can be obtained.

In Production Method 2-1, compound [15] is subjected to an alkylation reaction by using sodium hydride and a C_1-6alkyl halide. Thereafter, the resultant product is subjected to the reaction of Step 2-1-11, whereby compound [I-a7] which is a compound represented by the formula [I] wherein ring Cy is the formula

[Image Omitted]

wherein R¹, R⁴and n are as defined in the aforementioned formula [I] and C_1-6Alkyl is as defined in the aforementioned Production Method 2-1, can be obtained.

In Production Method 2-1, compound [16] is subjected to an amination reaction by using HNR^b8R^b9such as dimethylamine, diethylamine and the like. Thereafter, the resultant product is subjected to the reaction of Step 2-1-11, whereby compound [I-a8] which is a compound represented by the formula [I] wherein ring Cy is the formula

[Image Omitted]

wherein R¹, R⁴, R^b8, R^b9and n are as defined in the aforementioned formula [I], can be obtained.

In Production Method 2-1, compound [16] is reacted by using sodium hydride, and HNR^b14C(O)R^b15such as N-methylacetamide, 2-pyrrolidinone and the like. Thereafter, the resultant product is subjected to the reaction of Step 2-1-11, whereby compound [I-a9] which is a compound represented by the formula [I] wherein ring Cy is the formula

[Image Omitted]

wherein R¹, R⁴, R^b14, R^b15and n are as defined in the aforementioned formula [I], can be obtained.

In Production Method 2-1, compound [16] is reacted by using sodium hydride and compound [33] represented by the formula

[Image Omitted]

wherein R^b17, m5 and m6 are as defined in the aforementioned formula [I]. Thereafter, the resultant product is subjected to the reaction of Step 2-1-11, whereby compound [I-a10] which is a compound represented by the formula [I] wherein ring Cy is the formula

[Image Omitted]

wherein R¹, R⁴, R^b17, n, m5 and m6 are as defined in the aforementioned formula [I], can be obtained.

In Production Method 2-1, compound [19] is reacted with ClC(O)NR^b6R^b7such as dimethylcarbamoyl chloride, diethylcarbamoyl chloride and the like in the presence of a base. Thereafter, the resultant product is subjected to the reaction of Step 2-1-11, whereby compound [I-a11] which is a compound represented by the formula [I] wherein ring Cy is the formula

[Image Omitted]

wherein R¹, R⁴, R^b6, R^b7and n are as defined in the aforementioned formula [I], can be obtained.

In Production Method 2-1, compound [19] is reacted with R^b11S(O)₂Cl such as methanesulfonyl chloride and the like in the presence of a base. Thereafter, the resultant product is subjected to the reaction of Step 2-1-11, whereby compound [I-a12] which is a compound represented by the formula [I] wherein ring Cy is the formula

[Image Omitted]

wherein R¹, R⁴, R^b11and n are as defined in the aforementioned formula [I], can be obtained.

In Production Method 2-1, compound [19] is reacted with R^b13OC(O)Cl such as ethyl chloroformate and the like in the presence of a base. Thereafter, the resultant product is subjected to the reaction of Step 2-1-11, whereby compound [I-a13] which is a compound represented by the formula [I] wherein ring Cy is the formula

[Image Omitted]

wherein R¹, R⁴, R^b13and n are as defined in the aforementioned formula [I], can be obtained.

In Production Method 2-3, compound [I-a14] which is a compound represented by the formula [I] wherein ring Cy is the formula

[Image Omitted]

wherein R¹and R⁴are as defined in the aforementioned formula [I], j and k are as defined in the aforementioned Production Method 2-2, can be obtained by subjecting compound [27] to the reaction of Step 2-3-8.

By Production Method 2-4, compound [I-a15] which is a compound represented by the formula [I] wherein ring Cy is the formula

[Image Omitted]

wherein R¹, R⁴, R^b16, m2, m3 and m4 are as defined in the aforementioned Production Method 2-1, can be obtained.

[Production Method 2-4]

[Image Omitted]

wherein R¹, R⁴, R^b16, m2, m3 and m4 are as defined in the aforementioned formula [I] and C_1-6Alkyl, L², P^v, t and Y are as defined in the above-mentioned Production Method 2-1.

(Step 2-4-1)

Compound [36] can be obtained by reacting compound [34] with compound [35] in a solvent in the presence of a base.

Examples of the base to be used for the reaction include, lithium diisopropylamide, lithium bis(trimethylsilyl)amide and the like base.

Examples of the solvent to be used for the reaction include ether solvents such as tetrahydrofuran, diethyl ether, 1,2-dimethoxyethane, cyclopentyl methyl ether and the like; hydrocarbon solvents such as toluene, hexane, xylene and the like, and a mixed solvent thereof.

Compound [35] may be a commercially available product such as benzyl chloromethyl ether, or may be obtained by converting a commercially available product as appropriate by a method well known to those of ordinary skill in the art.

(Step 2-4-2)

Compound [37] can be obtained by removing P^vof compound [36] in the same manner as in Production Method 2-1, Step 2-1-6.

(Step 2-4-3)

Compound [38] can be obtained by converting the ester of compound [37] to carboxy by hydrolysis in the same manner as in Production Method 2-1, Step 2-1-1.

(Step 2-4-4)

Compound [39] can be obtained by reacting compound [38] with compound [19] in a solvent in the presence of a condensing agent and an additive in the same manner as in Production Method 2-1, Step 2-1-10.

(Step 2-4-5)

Compound [40] can be obtained by cyclization of compound [39] by intramolecular Mitsunobu reaction. For example, compound [40] can be obtained by reacting compound [39] with an azodicarboxylic acid diester (e.g., diethyl azodicarboxylate, diisopropyl azodicarboxylate, bis(2-methoxyethyl) azodicarboxylate etc.) in a solvent in the presence of a phosphine such as triphenylphosphine, tributylphosphine and the like.

Examples of the solvent to be used for the reaction include dichloromethane, chloroform, 1,4-dioxane, tetrahydrofuran, diethyl ether, 1,2-dimethoxyethane, cyclopentyl methyl ether, toluene, N,N-dimethylformamide and the like. These may be used singly or as a mixture of two or more kinds thereof.

[Production Method 3-1]

Another method of appropriately converting the substituent of ring Cy is, for example, Production Method 3-1 for obtaining compound [I-b1] which is a compound represented by the formula [I] wherein ring Cy is the formula

[Image Omitted]

wherein R¹, R⁴and R^care as defined in the aforementioned formula [I].

[Production Method 3-1]

[Image Omitted]

wherein R¹, R⁴, R⁵, R^c, m1 and X are as defined in the aforementioned formula [I], Z is as defined in the above-mentioned Production Method 1-1, and Hal²and P^vare as defined in the above-mentioned Production Method 2-1.

(Step 3-1-1)

Compound [42] can be obtained by protecting the hydroxy group of compound [41] in the same manner as in Production Method 2-1, Step 2-1-3.

Compound [41] may be a commercially available product such as 2-bromo-3-methylphenol, or may be obtained by converting a commercially available product as appropriate by a method well known to those of ordinary skill in the art.

(Step 3-1-2)

Compound [43] can be obtained by borating compound [42] in the same manner as in Production Method 1-3, Step 1-3.

(Step 3-1-3)

Compound [44] can be obtained by the Suzuki coupling reaction of compound [3] and compound [43] in the same manner as in Production Method 1-1, Step 1-1-2.

(Step 3-1-4)

Compound [45] can be obtained by removing P^vof compound [44] in the same manner as in Production Method 2-1, Step 2-1-6.

(Step 3-1-5)

Compound [47] can be obtained by the Mitsunobu reaction of compound [45] and compound [46]. For example, compound [47] can be obtained by reacting compound [45] with compound [46] in a solvent in the presence of an azodicarboxylic acid diester (e.g., diethyl azodicarboxylate, diisopropyl azodicarboxylate, bis(2-methoxyethyl) azodicarboxylate etc.) and a phosphine such as triphenylphosphine, tributylphosphine and the like.

Examples of the solvent to be used for the reaction include dichloromethane, chloroform, 1,4-dioxane, tetrahydrofuran, diethyl ether, 1,2-dimethoxyethane, cyclopentyl methyl ether, toluene, N,N-dimethylformamide and the like. These may be used singly or as a mixture of two or more kinds thereof.

Compound [46] may be a commercially available product such as benzyl alcohol, 2-pyridinemethanol and the like, or may be obtained by converting a commercially available product as appropriate by a method well known to those of ordinary skill in the art.

(Step 3-1-6)

Compound [I-b1] can be obtained by converting the alkoxy of compound [47] to hydroxy by hydrolysis in the same manner as in Production Method 1-1, Step 1-1-3.

In Production Method 3-1, for example, compound [I-b2]which is a compound represented by the formula [I] wherein ring Cy is the formula

[Image Omitted]

wherein R¹and R⁴are as defined in the aforementioned formula [I], can be obtained by subjecting compound [45] to the reaction of Step 3-1-6.

[Production Method 3-2]

Compound [43a] which is compound [43] wherein R¹is chloro or trifluoromethyl can also be obtained by [Production Method 3-2].

[Production Method 3-2]

[Image Omitted]

wherein R^yis chloro or trifluoromethyl;
R⁴is as defined in the aforementioned formula [I], Z is as defined in the aforementioned Production Method 1-1, and P^vis as defined in the aforementioned Production Method 2-1.

(Step 3-2-1)

Compound [49] can be obtained by protecting the hydroxy group of compound [48] in the same manner as in Production Method 2-1, Step 2-1-3.

In one embodiment, compound [48] may be a commercially available product such as those shown below, or may be obtained by converting a commercially available product as appropriate by a method well known to those of ordinary skill in the art.

[Image Omitted]

(Step 3-2-2)

Compound [43a] can be obtained by reacting compound [49] with a boron compound in a solvent in the presence of a base. For example, compound [43a] can be obtained by adding a base to compound [49] in a solvent at âˆ’78Â° C. to room temperature, and reacting the resultant product with a boron reagent at âˆ’78Â° C. to room temperature.

Examples of the base to be used for the reaction include n-butyllithium, sec-butyllithium and the like.

Examples of the boron reagent to be used for the reaction include 2-isopropoxy-4,4,5,5-tetramethyl-1,3,2-dioxaborolane, trimethyl borate and the like.

Examples of the solvent to be used for the reaction include tetrahydrofuran, diethyl ether, 1,2-dimethoxyethane, cyclopentyl methyl ether and the like.

[Production Method 4]

For example, compound [I-c1] of the formula

[Image Omitted]

wherein R⁵, R^e, X and Cy are as defined in the aforementioned formula [I], m7 is 0, 1 or 2, and when m7 is 2, each R⁵is selected independently, can be obtained by appropriately converting the substituent of compound [2].

[Production Method 4]

[Image Omitted]

wherein L³is a leaving group such as trifluoromethanesulfonyloxy and the like;
P^xis a hydroxy-protecting group such as benzyl and the like;
R⁵, R⁶, R^e, X and Cy are as defined in the aforementioned formula [I], Hal¹and Z are as defined in the aforementioned Production Method 1-1, and m7 is as defined in the aforementioned formula [I-A].

(Step 4-1)

Compound [51] can be obtained by the Suzuki coupling reaction of compound [1] and compound [50] in the same manner as in Production Method 1-1, Step 1-1-1.

Compound [50] may be a commercially available product such as 4-(benzyloxy)phenylboronic acid, or may be obtained by converting a commercially available product as appropriate by a method well known to those of ordinary skill in the art.

(Step 4-2)

Compound [52] can be obtained by the Suzuki coupling reaction of compound [4] and compound [51] in the same manner as in Production Method 1-1, Step 1-1-2.

(Step 4-3)

Compound [53] can be obtained by removing the phenol protecting group P^xof compound [52]. The deprotection can be performed by a known method according to the protecting group to be employed.

For example, when P^xis benzyl, compound [52] only needs to be subjected to a hydrogenation reaction in a single or mixed solvent of tetrahydrofuran, ethyl acetate, ethanol, methanol, water and the like in the presence of a catalyst such as palladium carbon, platinum carbon and the like.

(Step 4-4)

Compound [54] can be obtained by converting the hydroxy to a leaving group L³. For example, when the leaving group is trifluoromethanesulfonyloxy, compound [54] can be obtained by reacting compound [53] with trifluoromethanesulfonic anhydride, N-phenyl bis(trifluoromethanesulfonimide) and the like in a solvent in the presence of a base from ice-cooling to room temperature.

Examples of the base to be used for the reaction include organic bases such as pyridine, 2,6-lutidine, triethylamine and the like; inorganic bases such as alkali metal salts (e.g., cesium carbonate, sodium hydride etc.) and the like.

Examples of the solvent to be used for the reaction include ether solvents such as 1,4-dioxane, tetrahydrofuran, diethyl ether, 1,2-dimethoxyethane, cyclopentyl methyl ether and the like; hydrocarbon solvents such as toluene, hexane, xylene and the like; halogenated solvents such as dichloromethane, chloroform and the like; polar solvents such as N,N-dimethylformamide, N,N-dimethylacetamide, dimethyl sulfoxide, acetonitrile, pyridine and the like, and the like. These may be used singly or as a mixture of two or more kinds thereof.

(Step 4-5)

Compound [56] can be obtained by the Sonogashira reaction of compound [54] and compound [55]. For example, compound [56] can be obtained by reacting compound [54] with compound [55] in a solvent preferably under heating in the presence of a base, a palladium catalyst and a copper catalyst.

Examples of the palladium catalyst to be used for the reaction include palladium acetate, tetrakistriphenylphosphinepalladium, bis(triphenylphosphine)palladium dichloride, (bis(diphenylphosphino)ferrocene)palladium dichloride-methylene chloride complex and the like.

Examples of the copper catalyst to be used for the reaction include copper iodide, copper bromide and the like.

Examples of the base to be used for the reaction include diethylamine, dicyclohexylamine, triethylamine, N-ethyldiisopropylamine and the like.

Examples of the solvent to be used for the reaction include ether solvents such as 1,4-dioxane, tetrahydrofuran, diethyl ether, 1,2-dimethoxyethane, cyclopentyl methyl ether and the like; hydrocarbon solvents such as toluene, hexane, xylene and the like; and polar solvents such as N,N-dimethylformamide, N,N-dimethylacetamide, dimethyl sulfoxide, acetonitrile, pyridine and the like. These may be used singly or as a mixture of two or more kinds thereof.

Compound [55] may be a commercially available product such as cyclohexylacetylene, 2-ethynylpyridine and the like, or may be obtained by converting a commercially available product as appropriate by a method well known to those of ordinary skill in the art.

As for the Sonogashira coupling reaction, for example, the following review article is known (NAJERA, C et al. The Sonogashira Reaction: A Booming Methodology in Synthetic Organic Chemistry. Chem Rev. 2007, Vol. 107, pages 874-922.).

(Step 4-6)

Compound [I-c1] can be obtained by converting the alkoxy of compound [56] to hydroxy by hydrolysis in the same manner as in Production Method 1-1, Step 1-1-3.

In Production Method 4, compound [I-c2] of the formula

[Image Omitted]

wherein R⁵, X and Cy are as defined in the aforementioned formula [I], and m7 is as defined in the aforementioned formula [I-A], can be obtained by subjecting compound [53] to the reaction of Step 4-6.

In Production Method 4, compound [I-c3] of the formula

[Image Omitted]

wherein R⁵, R^d, X and Cy are as defined in the aforementioned formula [I], and m7 is as defined in the aforementioned formula [I-A], can be obtained by the Mitsunobu reaction of compound [53] and R^dOH such as cyclohexylmethanol and the like in the same manner as in Production Method 3-1, Step 3-1-5, and subjecting the resultant product to the reaction of Step 4-6.

In Production Method 4, the Suzuki coupling reaction of compound [54] and compound [57] of the formula

[Image Omitted]

wherein Z is as defined in the aforementioned Production Method 1-1, is performed in the same manner as in Production Method 1-1, Step 1-1-2. The resultant product is subjected to the reaction of Step 4-6, whereby compound [I-c4] of the formula

[Image Omitted]

wherein R⁵, X and Cy are as defined in the aforementioned formula [I] and m7 is as defined in the aforementioned formula [I-A], can be obtained.

In Production Method 4, the Suzuki coupling reaction of compound [54] and compound [58] of the formula

[Image Omitted]

wherein m9 is 1, 2, 3, or 4, and Z is as defined in the aforementioned Production Method 1-1, is performed in the same manner as in Production Method 1-1, Step 1-1-2. After reduction of the olefin of the resultant product, the obtained product is subjected to the reaction of Step 4-6, whereby compound [I-c5] of the formula

[Image Omitted]

wherein m9 is as defined above, m7 is as defined in the aforementioned formula [I-A], and R⁵, X and Cy are as defined in the aforementioned formula [I], can be obtained. For reduction reaction of the olefin, for example, a hydrogenation reaction only needs to be performed in a single or mixed solvent of tetrahydrofuran, ethyl acetate, ethanol, methanol, water and the like in the presence of a catalyst such as palladium carbon or platinum carbon and the like.

EXAMPLES

The present invention is explained in more detail in the following by referring to Examples and Experimental Examples, which are not to be construed as limitative.

The abbreviations in the Examples are as follows.

WSC.HCl: 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride
HOBt.H₂O: 1-hydroxy-1H-benzotriazole monohydrate
DMSO: dimethyl sulfoxide
M: mol/L

Production Example 1Synthesis of N-(4-chloro-3-{4-[4-(2,2-dimethylpropoxyl)phenyl]-6-hydroxy-1,3,5-triazin-2-yl}benzyl)-3,3,3-trifluoro-2,2-dimethylpropionamide (Example No. 1-86)

[Image Omitted]

(1) 2-chloro-4-[4-(2,2-dimethylpropoxyl)phenyl]-6-methoxy-1,3,5-triazine

[Image Omitted]

Under an argon atmosphere, a suspension of 4-(2,2-dimethylpropoxyl)phenylboronic acid (2.0 g, 9.6 mmol), 2,4-dichloro-6-methoxy-1,3,5-triazine (3.5 g, 19 mmol), [1,1â€²-bis(diphenylphosphino) ferrocene]palladium(II)dichloride dichloromethane adduct (1.1 g, 0.96 mmol) and 2M aqueous sodium carbonate solution (14 ml, 29 mmol) in toluene (20 ml) was stirred at 100Â° C. for 3.5 hr. At room temperature, to the reaction mixture were added water and ethyl acetate and the mixture was partitioned. The organic layer was washed with saturated aqueous sodium hydrogen carbonate, washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=19/1âˆ’4/1) to give the title compound (2.3 g, yield 77%).

¹H-NMR (400 MHz, CDCl₃) Î´: 1.06 (9H, s), 3.68 (2H, s), 4.14 (3H, s), 6.94-7.02 (2H, m), 8.42-8.46 (2H, m).

(2) (4-chloro-3-{4-[4-(2,2-dimethylpropoxyl)phenyl]-6-methoxy-1,3,5-triazin-2-yl}phenyl)methanol

[Image Omitted]

Under an argon atmosphere, a suspension of 2-chloro-4-[4-(2,2-dimethylpropoxyl)phenyl]-6-methoxy-1,3,5-triazine (2.3 g, 7.4 mmol) obtained in the above-mentioned (1), 2-chloro-5-hydroxymethylphenylboronic acid (1.7 g, 8.9 mmol), [1,1â€²-bis(diphenylphosphino)ferrocene]palladium(II)dichloride dichloromethane adduct (0.61 g, 0.74 mmol) and 2M aqueous sodium carbonate solution (15 ml, 30 mmol) in 1,4-dioxane (23 ml) was stirred at 100Â° C. for 1.5 hr. At room temperature, to the reaction mixture were added water and ethyl acetate and the mixture was partitioned. The organic layer was washed with saturated aqueous sodium hydrogen carbonate, washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=4/1âˆ’1/1) to give the title compound (1.3 g, yield 43%).

¹H-NMR (400 MHz, CDCl₃) Î´: 1.06 (9H, s), 1.75 (1H, t, J=5.9 Hz), 3.69 (2H, s), 4.19 (3H, s), 4.77 (2H, d, J=5.9 Hz), 6.98-7.03 (2H, m), 7.46 (1H, dd, J=8.2, 2.2 Hz), 7.53 (1H, d, J=8.2 Hz), 8.00 (1H, d, J=2.2 Hz), 8.52-8.58 (2H, m).

(3) tert-butyl N-(4-chloro-3-{4-[4-(2,2-dimethylpropoxyl)phenyl]-6-methoxy-1,3,5-triazin-2-yl}benzyl)-N-(tert-butoxycarbonyl)carbamate

[Image Omitted]

Under an argon atmosphere, to a solution of (4-chloro-3-20 {4-[4-(2,2-dimethylpropoxy)phenyl]-6-methoxy-1,3,5-triazin-2-yl}phenyl)methanol (1.3 g, 3.2 mmol) obtained in the above-mentioned (2) and triethylamine (0.58 ml, 4.2 mmol) in tetrahydrofuran (13 ml) was added methanesulfonyl chloride (0.29 ml, 3.8 mmol) under ice-cooling, and the mixture was warmed to room temperature. After stirring for 0.5 hr, the reaction mixture was filtered, and the filtrate was concentrated under reduced pressure. To a solution of the residue in N,N-dimethylformamide (13 ml) were added cesium carbonate (3.1 g, 9.5 mmol) and di-tert-butyl iminodicarboxylate (0.83 g, 3.8 mmol), and the mixture was stirred for 3 hr. To the reaction mixture were added water and ethyl acetate and the mixture was partitioned. The organic layer was washed with saturated aqueous sodium hydrogen carbonate, washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=9/1âˆ’7/3) to give the title compound (1.6 g, yield 82%).

¹H-NMR (400 MHz, CDCl₃) Î´: 1.06 (9H, s), 1.48 (18H, s), 3.69 (2H, s), 4.18 (3H, s), 4.83 (2H, s), 6.96-7.01 (2H, m), 7.39 (1H, dd, J=8.2, 2.2 Hz), 7.48 (1H, d, J=8.2 Hz), 7.98 (1H, d, J=2.2 Hz), 8.51-8.57 (2H, m).

(4) 4-chloro-3-{4-[4-(2,2-dimethylpropoxyl)phenyl]-6-methoxy-1,3,5-triazin-2-yl}benzylamine hydrochloride

[Image Omitted]

Under an argon atmosphere, to a solution of tert-butyl N-(4-chloro-3-{4-[4-(2,2-dimethylpropoxyl)phenyl]-6-methoxy-1,3,5-triazin-2-yl}benzyl)-N-(tert-butoxycarbonyl)carbamate (1.3 g, 2.2 mmol) obtained in the above-mentioned (3) in 1,4-dioxane (2.8 ml) was added 4M hydrogen chloride/1,4-dioxane solution (11 ml) at room temperature, and the mixture was stirred for 3 hr. The solid was collected by filtration from the suspension, and dried under reduced pressure to give the title compound (0.97 g, yield 99%).

¹H-NMR (400 MHz, DMSO-d₆) Î´: 1.03 (9H, s), 3.76 (2H, s), 4.10-4.18 (2H, m), 4.14 (3H, s), 7.11-7.17 (2H, m), 7.72 (2H, d, J=0.9 Hz), 8.13 (1H, br s), 8.40-8.58 (5H, m).

(5) N-(4-chloro-3-{4-[4-(2,2-dimethylpropoxyl)phenyl]-6-methoxy-1,3,5-triazin-2-yl}benzyl)-3,3,3-trifluoro-2,2-dimethylpropionamide

[Image Omitted]

Under an argon atmosphere, to a solution of 4-chloro-3-{4-[4-(2,2-dimethylpropoxyl)phenyl]-6-methoxy-1,3,5-triazin-2-yl}benzylamine hydrochloride (0.97 g, 2.2 mmol) obtained in the above-mentioned (4) and 3,3,3-trifluoro-2,2-dimethylpropionic acid (0.41 g, 2.6 mmol) in N,N-dimethylformamide (10 ml) were added HOBt.H₂O (0.43 g, 2.8 mmol), WSC.HCl (2.8 g, 2.8 mmol) and triethylamine (0.91 ml, 6.5 mmol) at room temperature, and the mixture was stirred for 3.5 hr. 3,3,3-Trifluoro-2,2-dimethylpropionic acid (0.067 g, 0.43 mmol), HOBt.H₂O (0.066 g, 0.43 mmol) and WSC.HCl (0.082 g, 0.43 mmol) were added, and the mixture was stirred for 1.5 hr. To the reaction mixture were added water and ethyl acetate and the mixture was partitioned. The organic layer was washed with saturated aqueous sodium hydrogen carbonate, washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=9/1âˆ’7/3) to give the title compound (0.97 g, yield 81%).

¹H-NMR (400 MHz, CDCl₃) Î´: 1.07 (9H, s), 1.44 (6H, s), 3.69 (2H, s), 4.19 (3H, s), 4.55 (2H, d, J=5.8 Hz), 6.22 (1H, br s), 6.96-7.03 (2H, m), 7.34 (1H, dd, J=8.3, 2.3 Hz), 7.51 (1H, d, J=8.3 Hz), 7.91 (1H, d, J=2.3 Hz), 8.50-8.57 (2H, m).

(6) N-(4-chloro-3-{4-[4-(2,2-dimethylpropoxyl)phenyl]-6-hydroxy-1,3,5-triazin-2-yl}benzyl)-3,3,3-trifluoro-2,2-dimethylpropionamide (Example No. 1-86)

[Image Omitted]

Under an argon atmosphere, to a solution of N-(4-chloro-3-{4-[4-(2,2-dimethylpropoxyl)phenyl]-6-methoxy-1,3,5-triazin-2-yl}benzyl)-3,3,3-trifluoro-2,2-dimethylpropionamide (0.97 g, 1.76 mmol) obtained in the above-mentioned (5) in methanol (10 ml) was added 4M aqueous sodium hydroxide solution (3.5 ml, 14 mmol) at room temperature, and the mixture was stirred at 65Â° C. for 1.5 hr. To the reaction mixture were added 2M hydrochloric acid (7.0 ml, 14 mmol) and water at room temperature, and the mixture was stirred. The precipitated solid was collected by filtration, washed with water, and dried under reduced pressure to give the title compound (0.87 g, yield 92%).

¹H-NMR (400 MHz, DMSO-d₆) Î´: 1.02 (9H, s), 1.37 (6H, s), 3.73 (2H, s), 4.35 (2H, d, J=5.8 Hz), 7.08 (2H, d, J=9.1 Hz), 7.40 (1H, dd, J=8.3, 2.2 Hz), 7.58 (1H, d, J=8.3 Hz), 7.62 (1H, d, J=1.9 Hz), 8.29 (2H, d, J=9.1 Hz), 8.62 (1H, t, J=5.8 Hz), 13.13 (1H, s).

Production Example 2Synthesis of 1-[4-chloro-3-(4-hydroxy-6-phenyl-1,3,5-triazin-2-yl)-benzyl]-3,3-dimethyl-1,3-dihydroindol-2-one (Example No. 1-258)

[Image Omitted]

(1) 2-(5-bromomethyl-2-chlorophenyl)-4-methoxy-6-phenyl-1,3,5-triazine

[Image Omitted]

By a method similar to that in Production Example 1 (1) and (2), and using 2,4-dichloro-6-methoxy-1,3,5-triazine, 2-chloro-5-hydroxymethylphenylboronic acid, and phenylboronic acid instead of 4-(2,2-dimethylpropoxyl)phenylboronic acid, [4-chloro-3-(4-methoxy-6-phenyl-1,3,5-triazin-2-yl)phenyl]methanol was obtained.

Under an argon atmosphere, to a solution of the obtained [4-chloro-3-(4-methoxy-6-phenyl-1,3,5-triazin-2-yl)phenyl]methanol (0.47 g, 1.4 mmol) and triphenylphosphine (0.56 g, 2.1 mmol) in chloroform (4.5 ml) was added carbon tetrabromide (0.71 g, 2.1 mmol) under ice-cooling. The reaction mixture was stirred at room temperature for 10 min, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=30/1âˆ’9/1) to give the title compound (0.49 g, yield 87%).

¹H-NMR (400 MHz, CDCl₃) Î´: 4.22 (3H, s), 4.53 (2H, s), 7.45-7.64 (5H, m), 8.06 (1H, br s), 8.57-8.63 (2H, m).

(2) 1-[4-chloro-3-(4-methoxy-6-phenyl-1,3,5-triazin-2-yl)benzyl]-3,3-dimethyl-1,3-dihydroindol-2-one

[Image Omitted]

Under an argon atmosphere, to a solution of 3,3-dimethylindolin-2-one (0.050 g, 0.31 mmol) in N,N-dimethylformamide (1.0 ml) was added sodium hydride (0.012 g, 60 wt % oil dispersion) under ice-cooling. After stirring for 30 min, 2-(5-bromomethyl-2-chlorophenyl)-4-methoxy-6-phenyl-1,3,5-triazine (0.10 g, 0.26 mmol) obtained in the above-mentioned (1) was added, and the mixture was stirred at room temperature for 30 min. To the reaction mixture were added saturated aqueous ammonium chloride solution and ethyl acetate and the mixture was partitioned. The organic layer was washed with saturated aqueous sodium hydrogen carbonate, washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=7/2) to give the title compound (0.11 g, yield 89%).

¹H-NMR (400 MHz, CDCl₃) Î´: 1.44 (6H, s), 4.18 (3H, s), 4.98 (2H, s), 6.72-6.76 (1H, m), 7.02-7.08 (1H, m), 7.13-7.19 (1H, m), 7.21-7.25 (1H, m), 7.31-7.36 (1H, m), 7.46-7.53 (3H, m), 7.55-7.61 (1H, m), 8.00 (1H, br s), 8.51-8.58 (2H, m).

(3) 1-[4-chloro-3-(4-hydroxy-6-phenyl-1,3,5-triazin-2-yl)benzyl]-3,3-dimethyl-1,3-dihydroindol-2-one (Example No. 1-258)

[Image Omitted]

Under an argon atmosphere, to a solution of 1-[4-chloro-3-(4-methoxy-6-phenyl-1,3,5-triazin-2-yl)benzyl]-3,3-dimethyl-1,3-dihydroindol-2-one (0.11 g, 0.23 mmol) obtained in the above-mentioned (2) in methanol (10 ml) was added 4M aqueous sodium hydroxide solution (0.34 ml, 1.4 mmol) at room temperature, and the mixture was stirred at 65Â° C. for 2 hr. To the reaction mixture were added 10 wt % aqueous citric acid solution (1.4 ml) and water (7.0 ml) at room temperature, and the mixture was stirred for 30 min. The precipitated solid was collected by filtration, washed with water, and dried under reduced pressure to give the title compound (0.10 g, yield 96%).

¹H-NMR (400 MHz, DMSO-d₆) Î´: 1.34 (6H, s), 4.99 (2H, s), 6.97 (1H, d, J=7.6 Hz), 7.05 (1H, t, J=7.6 Hz), 7.20 (1H, t, J=7.6 Hz), 7.39 (1H, d, J=7.6 Hz), 7.48 (1H, dd, J=8.3, 1.8 Hz), 7.55 (2H, t, J=7.6 Hz), 7.59-7.68 (2H, m), 7.75 (1H, d, J=1.8 Hz), 8.29 (2H, d, J=7.6 Hz), 13.32 (1H, br s).

Production Example 3Synthesis of N-[4-chloro-3-(4-hydroxy-6-phenyl-1,3,5-triazin-2-yl)benzyl]-N-ethyl-3,3,3-trifluoro-2,2-dimethylpropionamide (Example No. 1-263)

[Image Omitted]

(1) [4-chloro-3-(4-methoxy-6-phenyl-1,3,5-triazin-2-yl)benzyl]ethylamine

[Image Omitted]

Under an argon atmosphere, to 2-(5-bromomethyl-2-chlorophenyl)-4-methoxy-6-phenyl-1,3,5-triazine (0.20 g, 0.51 mmol) obtained in the same manner as in Production Example 2 (1) was added a solution of 2M ethylamine tetrahydrofuran (2.5 ml) at room temperature, and the mixture was stirred for 1 hr. To the reaction mixture were added saturated aqueous sodium hydrogen carbonate and ethyl acetate and the mixture was partitioned. The organic layer was washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure to give the title compound (0.28 g) as a crude product.

¹H-NMR (400 MHz, CDCl₃) Î´: 1.14 (3H, t, J=7.2 Hz), 2.70 (2H, q, J=7.2 Hz), 3.86 (2H, s), 4.22 (3H, s), 7.44 (1H, dd, J=8.2, 2.2 Hz), 7.48-7.55 (3H, m), 7.57-7.62 (1H, m), 7.97 (1H, d, J=2.2 Hz), 8.58-8.64 (2H, m).

(2) N-[4-chloro-3-(4-methoxy-6-phenyl-1,3,5-triazin-2-yl)benzyl]-N-ethyl-3,3,3-trifluoro-2,2-dimethylpropionamide

[Image Omitted]

Under an argon atmosphere, to a solution of [4-chloro-3-(4-methoxy-6-phenyl-1,3,5-triazin-2-yl)benzyl]ethylamine (0.18 g, 0.38 mmol) obtained in the above-mentioned (1) and 3,3,3-trifluoro-2,2-dimethylpropionic acid (0.12 g, 0.76 mmol) in chloroform (2.0 ml) were added WSC.HCl (0.15 g, 0.76 mmol) and 4-dimethylaminopyridine (0.93 mg, 0.76 mmol) at room temperature, and the mixture was stirred for 16 hr. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=8/3) to give the title compound (0.086 g, yield 46%).

¹H-NMR (400 MHz, CDCl₃) Î´: 1.20 (3H, t, J=6.9 Hz), 1.55 (6H, s), 3.47 (2H, q, J=6.9 Hz), 4.21 (3H, s), 4.71 (2H, s), 7.24-7.30 (1H, m), 7.45-7.63 (4H, m), 7.88 (1H, br s), 8.56-8.64 (2H, m).

(3) N-[4-chloro-3-(4-hydroxy-6-phenyl-1,3,5-triazin-2-yl)benzyl]-N-ethyl-3,3,3-trifluoro-2,2-dimethylpropionamide (Example No. 1-263)

[Image Omitted]

Under an argon atmosphere, to a solution of N-[4-chloro-3-(4-methoxy-6-phenyl-1,3,5-triazin-2-yl)benzyl]-N-ethyl-3,3,3-trifluoro-2,2-dimethylpropionamide (0.086 g, 0.17 mmol) obtained in the above-mentioned (2) in methanol (1.5 ml) was added 4M aqueous sodium hydroxide solution (0.26 ml, 1.0 mmol) at room temperature, and the mixture was stirred at 65Â° C. for 2 hr. At room temperature, 10 wt % aqueous citric acid solution (1.2 ml) and water (6 ml) were added, and the mixture was stirred for 30 min. The precipitated solid was collected by filtration, washed with water, and dried under reduced pressure to give a crude product. To a suspension of the crude product in ethyl acetate (1.5 ml) was added n-hexane (1.5 ml), and the mixture was stirred for 30 min. The solid was collected by filtration, dried under reduced pressure to give the title compound (0.067 g, yield 80%).

¹H-NMR (400 MHz, DMSO-d₆) Î´: 1.13 (3H, t, J=6.9 Hz), 1.50 (6H, s), 3.42 (2H, br s), 4.66 (2H, s), 7.41 (1H, dd, J=8.3, 1.8 Hz), 7.56 (2H, t, J=7.9 Hz), 7.61-7.69 (3H, m), 8.34 (2H, d, J=7.9 Hz), 13.33 (1H, br s).

Production Example 4Synthesis of 7-tert-butyl-2-[4-chloro-3-(4-hydroxy-6-phenyl-1,3,5-triazin-2-yl)benzyl]-2-azaspiro[3.5]nonan-1-one (Example No. 1-266)

[Image Omitted]

(1) methyl 1-benzyloxymethyl-4-tert-butyl-cyclohexanecarboxylate

[Image Omitted]

Under an argon atmosphere, to a solution of methyl 4-tert-butyl-cyclohexanecarboxylate (0.46 g, 2.3 mmol) in tetrahydrofuran (2.5 ml) was added dropwise 2M heptane/tetrahydrofuran/ethylbenzene solution (1.4 ml, 2.8 mmol) of lithium diisopropylamide at âˆ’78Â° C. over 5 min. After stirring for 1 hr, benzyl chloromethyl ether (0.38 ml, 2.8 mmol) was added dropwise over 1 min. Under ice-cooling, the mixture was stirred for 1 hr. To the reaction mixture were added 10 wt % aqueous citric acid solution (3.0 ml) and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated aqueous sodium hydrogen carbonate, washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=30/1) to give the title compound (0.49 g, yield 66%). While the title compound was obtained as a single stereoisomer, the relative configuration thereof is undetermined. Specifically, whether the methoxycarbonyl group is cis/trans relative to the tert-butyl group is undetermined.

¹H-NMR (400 MHz, CDCl₃) Î´: 0.81 (9H, s), 0.88-0.99 (1H, m), 1.00-1.21 (4H, m), 1.68 (2H, d, J=12.0 Hz), 2.29 (2H, d, J=12.0 Hz), 3.36 (2H, s), 3.69 (3H, s), 4.48 (2H, br s), 7.22-7.38 (5H, m).

(2) methyl 4-tert-butyl-1-hydroxymethyl-cyclohexanecarboxylate

[Image Omitted]

Under an argon atmosphere, to a solution of methyl 1-benzyloxymethyl-4-tert-butyl-cyclohexanecarboxylate (0.49 g, 1.5 mmol) obtained in the above-mentioned (1) in methanol (5.5 ml) was added ASCA-2 (4.5% palladium of activated carbon support-0.5% platinum catalyst (see N.E. CHEMCAT, Fine chemical Oct. 1, 2002, pages 5-14), 0.20 g) at room temperature. Under 1 atm hydrogen, the mixture was stirred for 4 hr. Under an argon atmosphere, the reaction mixture was filtered through celite, and the filtrate was eluted with ethyl acetate. The filtrate was concentrated under reduced pressure to give the title compound (0.27 g, yield 75%). While the title compound is a single stereoisomer, the relative configuration thereof is undetermined.

¹H-NMR (400 MHz, CDCl₃) Î´: 0.83 (9H, s), 0.91-1.17 (5H, m), 1.64-1.78 (3H, m), 2.20-2.31 (2H, m), 3.53 (2H, d, J=6.0 Hz), 3.73 (3H, s).

(3) 4-tert-butyl-1-hydroxymethyl-cyclohexanecarboxylic acid

[Image Omitted]

Under an argon atmosphere, to a solution of methyl 4-tert-butyl-1-hydroxymethyl-cyclohexanecarboxylate (0.27 g, 1.2 mmol) obtained in the above-mentioned (2) in methanol (1.7 ml) were added tetrahydrofuran (1.7 ml) and 4M aqueous sodium hydroxide solution (1.7 ml, 7.0 mmol) at room temperature, and the mixture was stirred at 65Â° C. for 1.5 hr. Methanol (1.7 ml), tetrahydrofuran (1.7 ml) and 4M aqueous sodium hydroxide solution (1.7 ml, 7.0 mmol) were added, and the mixture was stirred at 65Â° C. for 2 hr. To the reaction mixture were added 2M hydrochloric acid (7.5 ml, 15 mmol) and water at room temperature, and the mixture was stirred. Ethyl acetate was added and the mixture was partitioned. The organic layer was washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=12/1) to give the title compound (0.24 g, yield 94%). While the title compound is a single stereoisomer, the relative configuration thereof is undetermined.

¹H-NMR (400 MHz, DMSO-d₆) Î´: 0.80 (9H, s), 0.86-1.12 (5H, m), 1.53-1.66 (2H, m), 2.00-2.13 (2H, m), 3.31 (2H, s).

(4) 4-tert-butyl-1-hydroxymethyl-cyclohexanecarboxylic acid 4-chloro-3-(4-methoxy-6-phenyl-1,3,5-triazin-2-yl)benzylamide

[Image Omitted]

By a method similar to that in Production Example 1 (1)-(4), and using 2,4-dichloro-6-methoxy-1,3,5-triazine, 2-chloro-5-hydroxymethylphenylboronic acid, and phenylboronic acid instead of 4-(2,2-dimethylpropoxyl)phenylboronic acid, 4-chloro-3-(4-methoxy-6-phenyl-1,3,5-triazin-2-yl)benzylamine hydrochloride was obtained.

Under an argon atmosphere, to a solution of the obtained 4-chloro-3-(4-methoxy-6-phenyl-1,3,5-triazin-2-yl)benzylamine hydrochloride (0.90 g, 0.25 mmol) and 4-tert-butyl-1-hydroxymethyl-cyclohexanecarboxylic acid (0.080 g, 0.37 mmol) obtained in the above-mentioned (3) in N,N-dimethylformamide (2.0 ml) were added HOBt.H₂O (0.057 g, 0.37 mmol), WSC.HCl (0.071 g, 0.37 mmol) and triethylamine (0.10 ml, 0.74 mmol) at room temperature, and the mixture was stirred for 13 hr. To the reaction mixture were added saturated aqueous sodium hydrogen carbonate and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated aqueous sodium hydrogen carbonate, washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=1/2âˆ’1/3) to give the title compound (0.11 g, yield 81%). While the title compound is a single stereoisomer, the relative configuration thereof is undetermined.

¹H-NMR (400 MHz, CDCl₃) Î´: 0.78 (9H, s), 0.94-1.22 (5H, m), 1.66-1.75 (2H, m), 2.22-2.30 (2H, m), 2.42 (1H, t, J=5.0 Hz), 3.52 (2H, d, J=5.0 Hz), 4.21 (3H, s), 4.57 (2H, d, J=5.8 Hz), 6.46 (1H, t, J=5.8 Hz), 7.38 (1H, dd, J=8.3, 2.3 Hz), 7.47-7.55 (3H, m), 7.57-7.62 (1H, m), 7.97 (1H, d, J=2.3 Hz), 8.57-8.62 (2H, m).

(5) 7-tert-butyl-2-[4-chloro-3-(4-methoxy-6-phenyl-1,3,5-triazin-2-yl)benzyl]-2-azaspiro[3.5]nonan-1-one

[Image Omitted]

Under an argon atmosphere, to a solution of 4-tert-butyl-1-hydroxymethyl-cyclohexanecarboxylic acid 4-chloro-3-(4-methoxy-6-phenyl-1,3,5-triazin-2-yl)benzylamide (0.11 g, 0.20 mmol) obtained in the above-mentioned (4) and triphenylphosphine (0.080 g, 0.30 mmol) in tetrahydrofuran (1.0 ml) was added bis(2-methoxyethyl) azodicarboxylate (0.071 g, 0.30 mmol) at room temperature, and the mixture was stirred for 1.5 hr. To the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated aqueous sodium hydrogen carbonate, washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by preparative thin layer chromatography (eluent: n-hexane/ethyl acetate=4/1) to give the title compound (0.068 g, yield 66%). While the title compound is a single stereoisomer, the relative configuration of the tert-butyl group is undetermined.

¹H-NMR (400 MHz, CDCl₃) Î´: 0.81-1.77 (7H, m), 0.87 (9H, s), 2.03-2.12 (2H, m), 2.87 (2H, br s), 4.21 (3H, s), 4.40 (2H, br s), 7.30-7.37 (1H, m), 7.48-7.64 (4H, m), 7.90 (1H, br s), 8.57-8.63 (2H, m)

(6) 7-tert-butyl-2-[4-chloro-3-(4-hydroxy-6-phenyl-1,3,5-triazin-2-yl)benzyl]-2-azaspiro[3.5]nonan-1-one (Example No. 1-266)

[Image Omitted]

Under an argon atmosphere, to a solution of 7-tert-butyl-2-[4-chloro-3-(4-methoxy-6-phenyl-1,3,5-triazin-2-yl)benzyl]-2-azaspiro[3.5]nonan-1-one (0.068 g, 0.13 mmol) obtained in the above-mentioned (5) in methanol (1.2 ml) was added 4M aqueous sodium hydroxide solution (0.20 ml, 0.81 mmol) at room temperature, and the mixture was stirred at 65Â° C. for 1.5 hr. At room temperature, to the reaction mixture were added 10 wt % aqueous citric acid solution (0.82 ml) and water (4.0 ml), and the mixture was stirred for 30 min. The precipitated solid was collected by filtration, washed with water, and dried under reduced pressure to give the title compound (0.062 g, yield 94%). While the title compound is a single stereoisomer, the relative configuration of the tert-butyl group is undetermined.

¹H-NMR (400 MHz, DMSO-d₆) Î´: 0.83 (9H, s), 0.90-0.99 (1H, m), 1.41-1.67 (6H, m), 1.96-2.03 (2H, m), 2.92 (2H, s), 4.38 (2H, s), 7.47 (1H, dd, J=8.3, 1.8 Hz), 7.56 (2H, t, J=7.6 Hz), 7.63-7.69 (3H, m), 8.34 (2H, d, J=7.6 Hz), 13.34 (1H, br s).

Production Example 5Synthesis of 4-[2-(6-methylpyridin-2-ylmethoxy)-6-trifluoromethylphenyl]-6-(4-phenylethynylphenyl)-1,3,5-triazin-2-ol hydrochloride (Example No. 2-98)

[Image Omitted]

(1) 2-bromo-1-methoxymethoxy-3-trifluoromethyl-benzene

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Under an argon atmosphere, to a solution of 2-bromo-3-fluorobenzotrifluoride (6.0 g, 25 mmol) and 2-(methylsulfonyl)ethanol (4.3 g, 35 mmol) in N,N-dimethylformamide (10 ml) was added sodium hydride (2.8 g, 60 wt % oil dispersion) in 3 portions under ice-cooling. After stirring at room temperature for 10 min, chloromethyl methyl ether (5.3 ml, 69 mmol) was added dropwise under ice-cooling. After stirring for 30 min, the mixture was stirred at room temperature for 15 min. Under ice-cooling, to the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated aqueous sodium hydrogen carbonate, washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=12/1) to give the title compound (5.0 g, yield 70%).

¹H-NMR (400 MHz, CDCl₃) Î´: 3.53 (3H, s), 5.29 (2H, s), 7.31-7.38 (3H, m).

(2) 2-(2-methoxymethoxy-6-trifluoromethylphenyl)-4,4,5,5-tetramethyl-1,3,2-dioxaborolane

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Under an argon atmosphere, to a solution of 2-bromo-1-methoxymethoxy-3-trifluoromethyl-benzene (4.9 g, 17 mmol) obtained in the above-mentioned (1) in tetrahydrofuran (90 ml) was added dropwise n-butyllithium (1.6M n-hexane solution, 11 ml, 17 mmol) at âˆ’78Â° C. over 30 min. 2-Isopropoxy-4,4,5,5-tetramethyl-1,3,2-dioxaborolane (3.5 ml, 17 mmol) was added dropwise over 15 min, and the mixture was warmed to room temperature and stirred for 2 hr. To the reaction mixture were added saturated ammonium chloride aqueous solution and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated aqueous sodium hydrogen carbonate, washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=9/1) to give the title compound (2.8 g, yield 48%).

¹H-NMR (400 MHz, CDCl₃) Î´: 1.39 (12H, s), 3.47 (3H, s), 5.18 (2H, s), 7.20 (1H, d, J=8.4 Hz), 7.24-7.28 (1H, m), 7.36-7.42 (1H, m).

(3) 2-(4-benzyloxyphenyl)-4-methoxy-6-(2-methoxymethoxy-6-trifluoromethylphenyl)-1,3,5-triazine

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By a method similar to that in Production Example 1 (1), and using 2,4-dichloro-6-methoxy-1,3,5-triazine, and 4-(benzyloxy)phenylboronic acid instead of 4-(2,2-dimethylpropoxyl)phenylboronic acid, 2-(4-benzyloxyphenyl)-4-chloro-6-methoxy-1,3,5-triazine was obtained.

Under an argon atmosphere, to a solution of the obtained 2-(4-benzyloxyphenyl)-4-chloro-6-methoxy-1,3,5-triazine (3.0 g, 9.2 mmol) and 2-(2-methoxymethoxy-6-trifluoromethylphenyl)-4,4,5,5-tetramethyl-1,3,2-dioxaborolane (2.8 g, 8.4 mmol) obtained in the above-mentioned (2) in N,N-dimethylformamide (25 ml) were added [1,1â€²-bis (diphenylphosphino) ferrocene]palladium(II)dichloride dichloromethane adduct (1.4 g, 1.7 mmol), copper(I) iodide (0.48 g, 2.5 mmol) and 2M aqueous sodium carbonate solution (13 ml, 25 mmol), and the mixture was stirred at 115Â° C. for 45 min. To the reaction mixture were added water and ethyl acetate. After stirring, the insoluble material was removed by celite filtration, and the filtrate was eluted with ethyl acetate. The filtrate was partitioned, and the organic layer was washed with saturated aqueous sodium hydrogen carbonate, washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=7/2) to give the title compound (2.0 g, yield 47%).

¹H-NMR (400 MHz, DMSO-d₆) Î´: 3.39 (3H, s), 4.14 (3H, s), 5.13 (2H, s), 5.15 (2H, s), 7.02-7.08 (2H, m), 7.30-7.46 (7H, m), 7.48-7.55 (1H, m), 8.47-8.52 (2H, m).

(4) 4-[4-methoxy-6-(2-methoxymethoxy-6-trifluoromethylphenyl)-1,3,5-triazin-2-yl]phenol

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Under an argon atmosphere, to a solution of 2-(4-benzyloxyphenyl)-4-methoxy-6-(2-methoxymethoxy-6-trifluoromethylphenyl)-1,3,5-triazine (2.0 g, 4.0 mmol) obtained in the above-mentioned (3) in ethyl acetate (10 ml) were added methanol (10 ml) and 10 wt % palladium carbon (0.49 g) at room temperature. Under 1 atm hydrogen, the mixture was stirred for 2 hr. Under an argon atmosphere, the reaction mixture was filtered through celite, and the filtrate was eluted with ethyl acetate. The filtrate was concentrated under reduced pressure to give the title compound (1.6 g, yield 97%).

¹H-NMR (400 MHz, CDCl₃) Î´: 3.39 (3H, s), 4.14 (4H, s), 5.13 (2H, s), 5.39 (1H, br s), 6.87-6.93 (2H, m), 7.40-7.45 (2H, m), 7.48-7.55 (1H, m), 8.43-8.48 (2H, m).

(5) trifluoromethanesulfonic acid 4-[4-methoxy-6-(2-methoxymethoxy-6-trifluoromethylphenyl)-1,3,5-triazin-2-yl]phenyl ester

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Under an argon atmosphere, to a solution of 4-[4-methoxy-6-(2-methoxymethoxy-6-trifluoromethylphenyl)-1,3,5-triazin-2-yl]phenol (1.6 g, 3.9 mmol) obtained in the above-mentioned (4) in pyridine (15 ml) was added dropwise trifluoromethanesulfonic anhydride (13 ml, 7.7 mmol) under ice-cooling, and the mixture was stirred at room temperature for 30 min. To the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated aqueous sodium hydrogen carbonate, washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=3/1) to give the title compound (2.0 g, yield 95%).

¹H-NMR (400 MHz, CDCl₃) Î´: 3.39 (3H, s), 4.17 (3H, s), 5.13 (2H, s), 7.37-7.48 (4H, m), 7.51-7.58 (1H, m), 8.61-8.67 (2H, m).

(6) 2-methoxy-4-(2-methoxymethoxy-6-trifluoromethylphenyl)-6-(4-phenylethynylphenyl)-1,3,5-triazine

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Under an argon atmosphere, to a solution of trifluoromethanesulfonic acid 4-[4-methoxy-6-(2-methoxymethoxy-6-trifluoromethylphenyl)-1,3,5-triazin-2-yl]phenyl ester (0.50 g, 0.93 mmol) obtained in the above-mentioned (5), bis(triphenylphosphine)palladium(II)dichloride (0.098 g, 0.139 mmol) and copper(I) iodide (0.053 g, 0.28 mmol) in N,N-dimethylformamide (5.0 ml) were added triethylamine (0.39 ml, 2.8 mmol) and ethynylbenzene (0.51 ml, 4.6 mmol), and the mixture was stirred at 65Â° C. for 2.5 hr. To the reaction mixture were added water and ethyl acetate. After stirring for 1 hr, the insoluble material was removed by celite filtration, and the filtrate was eluted with ethyl acetate. The filtrate was partitioned, and the organic layer was washed with saturated aqueous sodium hydrogen carbonate, washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=5/1âˆ’4/1) to give the title compound (0.45 g, yield 98%).

¹H-NMR (400 MHz, DMSO-d₆) Î´: 3.40 (3H, s), 4.17 (3H, s), 5.14 (2H, s), 7.34-7.39 (3H, m), 7.42-7.47 (2H, m), 7.50-7.59 (3H, m), 7.62-7.67 (2H, m), 8.50-8.55 (2H, m).

(7) 2-[4-methoxy-6-(4-phenylethynylphenyl)-1,3,5-triazin-2-yl]-3-trifluoromethylphenol

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Under an argon atmosphere, to a solution of 2-methoxy-4-(2-methoxymethoxy-6-trifluoromethylphenyl)-6-(4-phenylethynylphenyl)-1,3,5-triazine (0.45 g, 0.92 mmol) obtained in the above-mentioned (6) in methanol (4.5 ml) were added 1,4-dioxane (4.5 ml) and methanesulfonic acid (0.030 ml, 0.46 mmol) at room temperature. The mixture was stirred at 70Â° C. for 5 hr, and triethylamine (0.13 ml, 0.92 mmol) was added to the reaction mixture at room temperature. To the reaction mixture was added water (45 ml), and the mixture was stirred for 30 min. The precipitated solid was collected by filtration and dried to give the title compound (0.38 g, yield 93%).

¹H-NMR (400 MHz, CDCl₃) Î´: 4.23 (3H, s), 7.25-7.30 (1H, m), 7.36-7.40 (3H, m), 7.43-7.47 (1H, m), 7.50-7.60 (3H, m), 7.67-7.72 (2H, m), 8.48-8.52 (2H, m), 12.43 (1H, br s)

(8) 2-methoxy-4-[2-(6-methylpyridin-2-ylmethoxy)-6-trifluoromethylphenyl]-6-(4-phenylethynylphenyl)-1,3,5-triazine

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Under an argon atmosphere, to a solution of 2-[4-methoxy-6-(4-phenylethynylphenyl)-1,3,5-triazin-2-yl]-3-trifluoromethylphenol (0.24 g, 0.54 mmol) obtained in the above-mentioned (7), 6-methyl-2-pyridinemethanol (0.099 g, 0.80 mmol) and triphenylphosphine (0.21 g, 0.80 mmol) in tetrahydrofuran (6.0 ml) was added bis(2-methoxyethyl) azodicarboxylate (0.19 g, 0.80 mmol) in 3 portions under ice-cooling. The reaction mixture was stirred for 20 min and at room temperature for 20 hr. Thereafter, to the reaction mixture were added 6-methyl-2-pyridinemethanol (0.099 g, 0.80 mmol) and triphenylphosphine (0.21 g, 0.80 mmol), and bis(2-methoxyethyl) azodicarboxylate (0.19 g, 0.80 mmol) in 2 portions under ice-cooling. After stirring for 20 min, the reaction mixture was stirred for 10 min at room temperature. To the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated aqueous sodium hydrogen carbonate, washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=4/3) to give the title compound (0.28 g, yield 95%).

¹H-NMR (400 MHz, CDCl₃) Î´: 2.51 (3H, s), 4.17 (3H, s), 5.21 (2H, s), 6.96-7.01 (1H, m), 7.02-7.07 (1H, m), 7.20-7.25 (1H, m), 7.33-7.42 (5H, m), 7.47-7.59 (3H, m), 7.62-7.68 (2H, m), 8.52-8.57 (2H, m).

(9) 4-[2-(6-methylpyridin-2-ylmethoxy)-6-trifluoromethylphenyl]-6-(4-phenylethynylphenyl)-1,3,5-triazin-2-ol

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Under an argon atmosphere, to a suspension of 2-methoxy-4-[2-(6-methylpyridin-2-ylmethoxy)-6-trifluoromethylphenyl]-6-(4-phenylethynylphenyl)-1,3,5-triazine (0.28 g, 0.52 mmol) obtained in the above-mentioned (8) in methanol (4.6 ml) were added 4M aqueous sodium hydroxide solution (0.77 ml, 3.1 mmol) and tetrahydrofuran (0.46 ml) at room temperature. At 65Â° C., the reaction mixture was stirred for 3.5 hr. To the reaction mixture were added 10 wt % aqueous citric acid solution (3.2 ml) and water (16 ml) at room temperature, and the mixture was stirred for 30 min. The precipitated solid was collected by filtration, washed with water, and dried under reduced pressure to give the title compound (0.27 g, yield 95%).

¹H-NMR (400 MHz, DMSO-d₆) Î´: 2.43 (3H, s), 5.31 (2H, s), 7.07-7.17 (2H, m), 7.43-7.49 (3H, m), 7.50-7.68 (5H, m), 7.69-7.82 (3H, m), 8.32-8.38 (2H, m), 13.63 (1H, br s).

(10) 4-[2-(6-methylpyridin-2-ylmethoxy)-6-trifluoromethylphenyl]-6-(4-phenylethynylphenyl)-1,3,5-triazin-2-ol hydrochloride (Example No. 2-98)

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Under an argon atmosphere, to a solution of 4-[2-(6-methylpyridin-2-ylmethoxy)-6-trifluoromethylphenyl]-6-(4-phenylethynylphenyl)-1,3,5-triazin-2-ol (0.27 g, 0.49 mmol) obtained in the above-mentioned (9) in 1,4-dioxane (5.3 ml) was added 4M hydrogen chloride/1,4-dioxane solution (0.37 ml, 1.5 mmol) at room temperature. To the reaction mixture was added n-hexane (21 ml), and the mixture was stirred for 30 min. The precipitated solid was collected by filtration, washed with n-hexane, and dried under reduced pressure to give the title compound (0.26 g, yield 91%).

¹H-NMR (400 MHz, DMSO-d₆) Î´: 2.48 (3H, s), 5.37 (2H, s), 7.23 (1H, d, J=7.3 Hz), 7.28 (1H, d, J=7.3 Hz), 7.48-7.45 (3H, m), 7.56 (1H, d, J=7.9 Hz), 7.64-7.59 (2H, m), 7.67 (1H, d, J=8.6 Hz), 7.82-7.72 (4H, m), 8.35 (2H, dd, J=6.8, 2.0 Hz).

Production Example 6Synthesis of 2-[4-chloro-2-methyl-5-(4,4,5,5-tetramethyl[1,3,2]dioxaborolan-2-yl)benzyloxy]tetrahydropyran(1) 4-chloro-5-iodo-2-methylbenzoic acid

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Under an argon atmosphere, to 4-chloro-2-methylbenzoic acid (1.9 g, 11 mmol) were added concentrated sulfuric acid (16 ml) and N-iodosuccinimide (2.7 g, 12 mmol) under ice-cooling, and the mixture was stirred at room temperature for 4 hr. The reaction mixture was carefully poured into ice water and the mixture was stirred. The precipitated solid was collected by filtration, washed with water, and dried under reduced pressure to give the title compound (3.3 g, yield 99%).

¹H-NMR (CDCl₃) Î´: 2.58 (3H, s), 7.38 (1H, br s), 8.50 (1H, s).

(2) (4-chloro-5-iodo-2-methylphenyl) methanol

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Under an argon atmosphere, to a solution of 4-chloro-5-iodo-2-methylbenzoic acid (2.4 g, 8.1 mmol) in tetrahydrofuran (12 ml) were added triethylamine (1.2 ml, 8.9 mmol) and isobutyl chloroformate (1.2 ml, 8.9 mmol) under ice-cooling, and the mixture was stirred for 30 min. At room temperature, the insoluble material was removed by filtration, and washed with tetrahydrofuran (36 ml). The filtrate was added dropwise to a solution of prepared sodium borohydride (0.92 g, 24 mmol) in water (4.5 ml) over 10 min under ice-cooling. After stirring at room temperature for 2 hr, to the reaction mixture was added sodium borohydride (0.30 g, 8.1 mmol), and the mixture was stirred for 1 hr. To the reaction mixture were added saturated aqueous ammonium chloride solution and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated aqueous sodium hydrogen carbonate, washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: chloroform/ethyl acetate=100/0âˆ’95/5) to give the title compound (2.0 g, yield 88%).

¹H-NMR (400 MHz, CDCl₃) Î´: 1.60 (1H, t, J=5.7 Hz), 2.26 (3H, s), 4.63 (2H, d, J=5.6 Hz), 7.25-7.26 (1H, m), 7.84 (1H, br S).

(3) 2-(4-chloro-5-iodo-2-methylbenzyloxy)tetrahydropyran

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Under an argon atmosphere, to a solution of (4-chloro-5-iodo-2-methylphenyl)methanol (2.0 g, 7.1 mmol) obtained in the above-mentioned (1) in chloroform (20 ml) were added pyridinium p-toluenesulfonate (0.27 mg, 1.1 mmol) and 3,4-dihydro-2H-pyran (0.97 ml, 11 mmol) at room temperature, and the mixture was stirred for 16 hr. The reaction mixture was concentrated under reduced pressure, and the residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=9/1) to give the title compound (2.6 g, yield 99%).

¹H-NMR (400 MHz, CDCl₃) Î´: 1.51-1.92 (6H, m), 2.26 (3H, s), 3.52-3.59 (1H, m), 3.85-3.91 (1H, m), 4.38 (1H, d, J=12.6 Hz), 4.67-4.72 (2H, m), 7.25 (1H, br s), 7.82 (1H, br s).

(4) 2-[4-chloro-2-methyl-5-(4,4,5,5-tetramethyl[1,3,2]dioxaborolan-2-yl)benzyloxy]tetrahydropyran

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Under an argon atmosphere, to a solution of 2-(4-chloro-5-iodo-2-methylbenzyloxy)tetrahydropyran (2.3 g, 6.2 mmol) obtained in the above-mentioned (2) in 1,4-dioxane (23 ml) were added biphenyl-2-yl-dicyclohexylphosphine (0.43 g, 1.2 mmol), palladium(II) acetate (0.070 g, 0.31 mmol), triethylamine (3.4 ml, 25 mmol) and 4,4,5,5-tetramethyl-1,3,2-dioxaborolane (2.7 ml, 18 mmol) at room temperature, and the mixture was stirred at 80Â° C. for 5 hr. Under ice-cooling, to the reaction mixture was added dropwise water, and ethyl acetate was added. After partitioning, the organic layer was washed with saturated aqueous sodium hydrogen carbonate, washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=98/2âˆ’80/20) to give the title compound (1.3 g, yield 60%).

¹H-NMR (400 MHz, CDCl₃) Î´: 1.36 (12H, s), 1.47-1.90 (6H, m), 2.34 (3H, s), 3.52-3.59 (1H, m), 3.88-3.95 (1H, m), 4.42 (1H, d, J=11.6 Hz), 4.67 (1H, t, J=3.5 Hz), 4.74 (1H, d, J=11.6 Hz), 7.18 (1H, br s), 7.63 (1H, br s).

Production Example 7Synthesis of tert-butyl-(4-chloro-3-iodo-2-methylbenzyloxy)dimethylsilane(1) 3-(tert-butyl-dimethylsilanyloxymethyl)-6-chloro-2-methylphenylamine

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Under an argon atmosphere, to a solution of 3-(tert-butyldimethylsilanyloxymethyl)-2-methyl-phenylamine (0.91 g, 3.6 mmol) in tetrahydrofuran (5.0 ml) was added N-chlorosuccinimide (0.48 g, 3.6 mmol) at room temperature. After stirring for 22 hr, to the reaction mixture was added n-hexane (10 ml), and the insoluble material was filtered off. The filtrate was concentrated, and purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=20/1) to give the title compound (0.18 g, yield 17%).

¹H-NMR (400 MHz, CDCl₃) Î´: 0.08 (6H, s), 0.92 (9H, s), 2.11 (3H, s), 4.01 (2H, br s), 4.60-4.69 (2H, m), 6.77 (1H, d, J=8.4 Hz), 7.11 (1H, d, J=8.4 Hz).

(2) tert-butyl-(4-chloro-3-iodo-2-methylbenzyloxy)dimethylsilane

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Under an argon atmosphere, to a solution of 3-(tert-butyl-dimethylsilanyloxymethyl)-6-chloro-2-methylphenylamine (0.18 g, 0.63 mmol) obtained in the above-mentioned (1) in acetonitrile (2.0 ml) were added iodine (0.19 g, 0.76 mmol) and tert-butyl nitrite (0.11 ml, 0.94 mmol) at room temperature, and the mixture was stirred at 65Â° C. for 30 min. At room temperature, to the reaction mixture were added water and ethyl acetate. After partitioning, the organic layer was washed with saturated aqueous sodium hydrogen carbonate, 10 wt % aqueous sodium thiosulfate solution, washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=40/1) to give the title compound (0.099 g, yield 40%).

¹H-NMR (400 MHz, CDCl₃) Î´: 0.10 (6H, s), 0.93 (9H, s), 2.47 (3H, s), 4.68 (2H, s), 7.30 (1H, d, J=8.4 Hz), 7.35 (1H, d, J=8.1 Hz).

Production Example 8Synthesis of 2-(6-chloro-2-methoxymethoxy-3-methylphenyl)-4,4,5,5-tetramethyl[1,3,2]dioxaborolane(1) 4-chloro-2-methoxymethoxy-1-methylbenzene

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Under an argon atmosphere, to a solution of 5-chloro-2-methylphenol (1.0 g, 7.0 mmol) in N,N-dimethylformamide (20 ml) was added sodium hydride (0.34 g, 60 wt % oil dispersion) under ice-cooling. After stirring for 15 min, the mixture was stirred at room temperature for 30 min. Under ice-cooling, chloromethyl methyl ether (0.64 ml, 8.4 mmol) was added, and the mixture was stirred for 30 min. To the reaction mixture were added water and diethyl ether, and the mixture was partitioned at room temperature. The organic layer was washed with water, washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/diethyl ether=25/1) to give the title compound (1.3 g, yield 96%).

¹H-NMR (400 MHz, CDCl₃) Î´: 2.20 (3H, s), 3.48 (3H, s), 5.18 (2H, s), 6.89 (1H, dd, J=7.9, 2.0 Hz), 7.03-7.07 (2H, m).

(2) 2-(6-chloro-2-methoxymethoxy-3-methylphenyl)-4,4,5,5-tetramethyl[1,3,2]dioxaborolane

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Under an argon atmosphere, to a solution of 4-chloro-2-methoxymethoxy-1-methylbenzene (0.75 g, 4.0 mmol) obtained in the above-mentioned (1) in tetrahydrofuran (20 ml) was added dropwise n-butyllithium (1.6M n-hexane solution, 2.5 ml, 4.0 mmol) at âˆ’78Â° C. over 5 min. After stirring for 30 min, 2-isopropoxy-4,4,5,5-tetramethyl-1,3,2-dioxaborolane (0.81 ml, 4.0 mmol) was added. After stirring for 2 hr, the stirring was discontinued, and the mixture was warmed to room temperature.

After 13 hr, to the reaction mixture were added saturated aqueous ammonium chloride solution and ethyl acetate, and the mixture was partitioned. Thereafter, the organic layer was washed with water, washed with saturated brine, dried over magnesium sulfate, filtered to remove magnesium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=12/1) to give the title compound (0.20 g, yield 15%).

¹H-NMR (400 MHz, CDCl₃) Î´: 1.40 (12H, s), 2.27 (3H, s), 3.55 (3H, s), 5.03 (2H, s), 7.01 (1H, d, J=8.2 Hz), 7.07-7.11 (1H, m).

Production Example 9Synthesis of N-{4-chloro-3-[4-(4-isobutylphenyl)-6-hydroxy-1,3,5-triazin-2-yl]benzyl}-2,2-dimethylpropionamide (Example No. 1-51)

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(1) 2-chloro-4-(4-isobutylphenyl)-6-methoxy-1,3,5-triazine

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Under an argon atmosphere, a suspension of 4-(2-methylpropyl)phenylboronic acid (35 g, 200 mmol), 2,4-dichloro-6-methoxy-1,3,5-triazine (46 g, 260 mmol), tetrakis(triphenylphosphine)palladium(0) (2.3 g, 2.0 mmol) and sodium carbonate (63 g, 590 mmol) in toluene (280 ml) and distilled water (280 ml) was stirred at 70Â° C. for 3.5 hr. At room temperature, to the reaction mixture were added water, ethyl acetate, and n-hexane, and the mixture was partitioned. The organic layer was washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure to give the title compound (60 g) as a crude product.

(2) {4-chloro-3-[4-(4-isobutylphenyl)-6-methoxy-1,3,5-triazin-2-yl]phenyl}methanol

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Under an argon atmosphere, a suspension of a crude product (60 g) of 2-chloro-4-(4-isobutylphenyl)-6-methoxy-1,3,5-triazine obtained in the above-mentioned (1), 2-chloro-5-hydroxymethylphenylboronic acid (44 g, 240 mmol), [1,1â€²-bis(diphenylphosphino) ferrocene]palladium(II)dichloride dichloromethane adduct (3.2 g, 3.9 mmol) and cesium fluoride (90 g, 590 mmol) in acetonitrile (440 ml) and distilled water (130 ml) was stirred at 67Â° C. for 2 hr. At room temperature, to the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with water, and the organic layer was washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=7/3âˆ’6/4) to give the title compound (57 g).

¹H-NMR (CDCl₃) Î´: 0.93 (6H, d, J=6.6 Hz), 1.77 (1H, t, J=6.1 Hz), 1.90-1.97 (1H, m), 2.57 (2H, d, J=7.3 Hz), 4.21 (3H, s), 4.77 (2H, d, J=6.1 Hz), 7.29 (2H, d, J=8.3 Hz), 7.47 (1H, dd, J=8.3, 2.1 Hz), 7.54 (1H, d, J=8.3 Hz), 8.01 (1H, d, J=2.1 Hz), 8.51 (2H, d, J=8.3 Hz).

(3) tert-butyl N-{4-chloro-3-[4-(4-isobutylphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzyl}-N-(tert-butoxycarbonyl)carbamate

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Under an argon atmosphere, to a solution of {4-chloro-3-[4-(4-isobutylphenyl)-6-methoxy-1,3,5-triazin-2-yl]phenyl}methanol (0.25 g, 0.64 mmol) obtained in the above-mentioned (2) and triphenylphosphine (0.25 g, 0.96 mmol) in chloroform (2.4 ml) was added carbon tetrabromide (0.32 g, 0.96 mmol) under ice-cooling. The reaction mixture was stirred at room temperature for 10 min. The reaction mixture was subject to silica gel column chromatography (eluent: n-hexane/ethyl acetate=30/1âˆ’10/1), and concentrated under reduced pressure. A solution of the residue in N,N-dimethylformamide (2.0 ml) was added to a solution of di-tert-butyl iminodicarboxylate (0.140 g, 0.64 mmol) and sodium hydride (0.026 g, 60 wt % oil dispersion) in N,N-dimethylformamide (1.0 ml) under ice-cooling, and the mixture was stirred at room temperature for 15 min. The reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with water, and the organic layer was washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=6/1) to give the title compound (0.27 g, yield 72%).

¹H-NMR (CDCl₃) Î´: 0.93 (6H, d, J=6.6 Hz), 1.47 (18H, s), 1.88-1.98 (1H, m), 2.57 (2H, d, J=7.3 Hz), 4.19 (3H, s), 4.83 (2H, s), 7.28 (2H, d, J=8.4 Hz), 7.39 (1H, dd, J=8.4, 2.3 Hz), 7.48 (1H, d, J=8.4 Hz), 8.00 (1H, d, J=2.3 Hz), 8.50 (2H, dt, J=8.4, 1.8 Hz).

(4) 4-chloro-3-[4-(4-isobutylphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzylamine hydrochloride

[Image Omitted]

Under an argon atmosphere, to tert-butyl N-{4-chloro-3-[4-(4-isobutylphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzyl}-N-(tert-butoxycarbonyl)carbamate (0.27 g, 0.46 mmol) obtained in the above-mentioned (3) was added 4M hydrogen chloride/1,4-dioxane solution (2.0 ml) at room temperature, and the mixture was stirred for 30 min. The solid was collected by filtration from the suspension, and dried under reduced pressure to give the title compound as a crude product (0.16 g).

(5) N-{4-chloro-3-[4-(4-isobutylphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzyl}-2,2-dimethylpropionamide

[Image Omitted]

Under an argon atmosphere, to a solution of a crude product (0.035 g) of 4-chloro-3-[4-(4-isobutylphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzylamine hydrochloride obtained in the above-mentioned (4), HOBt.H₂O (0.019 g, 0.12 mmol) and WSC.HCl (0.024 g, 0.13 mmol) in N,N-dimethylformamide (1.0 ml) were added 2,2-dimethylpropionic acid (0.014 ml, 0.12 mmol) and triethylamine (0.035 ml, 0.25 mmol) at room temperature, and the mixture was stirred for 3 hr. To the reaction mixture were added saturated aqueous sodium hydrogen carbonate and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated aqueous sodium hydrogen carbonate, washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=3/2) to give the title compound (0.030 g).

¹H-NMR (CDCl₃) Î´: 0.93 (6H, d, J=6.6 Hz), 1.24 (9H, s), 1.88-1.99 (1H, m), 2.57 (2H, d, J=7.1 Hz), 4.20 (3H, s), 4.50 (2H, d, J=6.0 Hz), 5.98 (1H, br s), 7.29 (2H, d, J=8.3 Hz), 7.36 (1H, dd, J=8.2, 2.3 Hz), 7.51 (1H, d, J=8.2 Hz), 7.92 (1H, d, J=2.3 Hz), 8.50 (2H, d, J=8.3 Hz).

(6) N-{4-chloro-3-[4-hydroxy-6-(4-isobutylphenyl)-1,3,5-triazin-2-yl]benzyl}-2,2-dimethylpropionamide (Example No. 1-51)

[Image Omitted]

Under an argon atmosphere, to a solution of N-{4-chloro-3-[4-(4-isobutylphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzyl}-2,2-dimethylpropionamide (0.030 g, 0.064 mmol) obtained in the above-mentioned (5) in methanol (10 ml) was added 4M aqueous sodium hydroxide solution (0.096 ml) at room temperature, and the mixture was stirred at room temperature for 16 hr. To the reaction mixture were added 10% aqueous citric acid solution (0.38 ml) and water (2.3 ml) at room temperature, and the mixture was stirred. The precipitated solid was collected by filtration, washed with water, and dried under reduced pressure to give the title compound (0.026 g, yield 90%). A suspension of the title compound (0.030 g) in DME (0.60 ml) was stirred at room temperature, and the solid was collected by filtration and dried to give the title compound as crystals (0.026 g).

Production Example 10Synthesis of N-{4-chloro-3-[4-(3-fluoro-4-methylphenyl)-6-hydroxy-1,3,5-triazin-2-yl]benzyl}-3,3,3-trifluoro-2,2-dimethylpropionamide (Example No. 1-81)

[Image Omitted]

(1) 2-chloro-4-(3-fluoro-4-methylphenyl)-6-methoxy-1,3,5-triazine

[Image Omitted]

Under an argon atmosphere, to a suspension of 3-fluoro-4-methylphenylboronic acid (0.43 g, 2.8 mmol), 2,4-dichloro-6-methoxy-1,3,5-triazine (1.0 g, 5.6 mmol) and tetrakis(triphenylphosphine)palladium(0) (0.16 g, 0.14 mmol) in toluene (8 ml) was added 2M aqueous tripotassium phosphate solution (4.0 ml) at room temperature, and the mixture was stirred at 100Â° C. for 3 hr. At room temperature, to the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with water, partitioned, washed with saturated brine, dried over magnesium sulfate, filtered to remove magnesium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/chloroform=2/3âˆ’1/2) to give the title compound (0.58 g, yield 81%).

¹H-NMR (CDCl₃) Î´: 2.37 (3H, d, J=2.1 Hz), 4.17 (3H, s), 7.32 (1H, t, J=7.9 Hz), 8.12 (1H, dd, J=10.7, 1.7 Hz), 8.19 (1H, dd, J=7.9, 1.7 Hz).

(2) {4-chloro-3-[4-(3-fluoro-4-methylphenyl)-6-methoxy-1,3,5-triazin-2-yl]phenyl}methanol

[Image Omitted]

Under an argon atmosphere, to a solution of 2-chloro-4-(3-fluoro-4-methylphenyl)-6-methoxy-1,3,5-triazine (0.58 g, 2.3 mmol) obtained in the above-mentioned (1), 2-chloro-5-hydroxymethylphenylboronic acid (0.51 g, 2.7 mmol) and [1,1â€²-bis(diphenylphosphino)ferrocene]palladium(II) dichloride dichloromethane adduct (0.18 g, 0.23 mmol) in 1,4-dioxane (9.0 ml) was added 2M aqueous sodium carbonate solution (4.5 ml), and the mixture was stirred at 100Â° C. for 2 hr. At room temperature, to the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated brine, dried over magnesium sulfate, filtered to remove magnesium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=4/3) to give the title compound (0.44 g, yield 53%).

¹H-NMR (CDCl₃) Î´: 1.76 (1H, t, J=5.8 Hz), 2.37 (3H, d, J=1.9 Hz), 4.21 (3H, s), 4.78 (2H, d, J=5.8 Hz), 7.33 (1H, t, J=7.9 Hz), 7.47 (1H, dd, J=8.1, 2.2 Hz), 7.54 (1H, d, J=8.1 Hz), 8.02 (1H, d, J=2.2 Hz), 8.23 (1H, dd, J=10.7, 1.6 Hz), 8.29 (1H, dd, J=7.9, 1.6 Hz).

(3) tert-butyl N-{4-chloro-3-[4-(3-fluoro-4-methylphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzyl}-N-(tert-butoxycarbonyl)carbamate

[Image Omitted]

Under an argon atmosphere, to a solution of {4-chloro-3-[4-(3-fluoro-4-methylphenyl)-6-methoxy-1,3,5-triazin-2-yl]phenyl}methanol (0.44 g, 1.2 mmol) obtained in the above-mentioned (2) in tetrahydrofuran (13 ml) were added triethylamine (0.22 ml, 1.6 mmol) and methanesulfonyl chloride (0.10 ml, 1.3 mmol) under ice-cooling, and the mixture was stirred for 0.5 hr. The reaction mixture was added to a solution of di-tert-butyl iminodicarboxylate (0.32 g, 1.5 mmol) and cesium carbonate (1.2 g, 3.6 mmol) in N,N-dimethylformamide (3.0 ml) at room temperature, and the mixture was stirred for 1 hr. To the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated brine, dried over magnesium sulfate, filtered to remove magnesium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=6/1) to give the title compound (0.64 g, yield 94%).

¹H-NMR (CDCl₃) Î´: 1.48 (18H, s), 2.37 (3H, d, J=1.6 Hz), 4.19 (3H, s), 4.83 (2H, s), 7.31 (1H, t, J=7.9 Hz), 7.40 (1H, dd, J=8.4, 2.3 Hz), 7.49 (1H, d, J=8.4 Hz), 8.00 (1H, d, J=2.3 Hz), 8.22 (1H, dd, J=10.7, 1.6 Hz), 8.28 (1H, dd, J=7.9, 1.6 Hz).

(4) 4-chloro-3-[4-(3-fluoro-4-methylphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzylamine hydrochloride

[Image Omitted]

Under an argon atmosphere, to a solution of tert-butyl N-{4-chloro-3-[4-(3-fluoro-4-methylphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzyl}-N-(tert-butoxycarbonyl)carbamate (0.64 g, 1.1 mmol) obtained in the above-mentioned (3) in 1,4-dioxane (2.0 ml) was added 4M hydrogen chloride/1,4-dioxane solution (6.0 ml) at room temperature, and the mixture was stirred for 2 hr. To the reaction mixture was added n-hexane (32 ml), and the mixture was stirred for 45 min. The solid was collected by filtration from the suspension, and dried under reduced pressure to give the title compound (0.45 g, yield 99%).

¹H-NMR (DMSO-D₆) Î´: 2.36 (3H, d, J=1.4 Hz), 4.13-4.19 (2H, m), 4.17 (3H, s), 7.55 (1H, t, J=8.0 Hz), 7.71 (1H, dd, J=8.1, 2.1 Hz), 7.75 (1H, d, J=8.1 Hz), 8.16-8.20 (2H, m), 8.27 (1H, dd, J=7.9, 1.6 Hz), 8.38 (3H, br s).

(5) N-{4-chloro-3-[4-(3-fluoro-4-methylphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzyl}-3,3,3-trifluoro-2,2-dimethylpropionamide

[Image Omitted]

Under an argon atmosphere, to a solution of 4-chloro-3-[4-(3-fluoro-4-methylphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzylamine hydrochloride (0.070 g, 0.18 mmol) obtained in the above-mentioned (4), HOBt.H₂O (0.041 g, 0.27 mmol) and 3,3,3-trifluoro-2,2-dimethylpropionic acid (0.042 g, 0.27 mmol) in N,N-dimethylformamide (1.0 ml) were added WSC.HCl (0.051 g, 0.27 mmol) and triethylamine (0.037 ml, 0.027 mmol) at room temperature, and the mixture was stirred for 1.5 hr. To the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated aqueous sodium hydrogen carbonate, washed with saturated brine, dried over magnesium sulfate, filtered to remove magnesium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=2/1) to give the title compound (0.080 g, yield 90%).

¹H-NMR (CDCl₃) Î´: 1.45 (6H, s), 2.37 (3H, d, J=1.9 Hz), 4.20 (3H, s), 4.55 (2H, d, J=5.8 Hz), 6.23 (1H, br s), 7.30-7.37 (2H, m), 7.52 (1H, d, J=8.4 Hz), 7.93 (1H, d, J=2.3 Hz), 8.22 (1H, dd, J=10.7, 1.6 Hz), 8.28 (1H, dd, J=7.9, 1.6 Hz).

(6) N-{4-chloro-3-[4-(3-fluoro-4-methyl-phenyl)-6-hydroxy-1,3,5-triazin-2-yl]benzyl}-3,3,3-trifluoro-2,2-dimethylpropionamide (Example No. 1-81)

[Image Omitted]

Under an argon atmosphere, to a solution of N-{4-chloro-3-[4-(3-fluoro-4-methylphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzyl}-3,3,3-trifluoro-2,2-dimethylpropionamide (0.077 g, 0.16 mmol) obtained in the above-mentioned (5) in methanol (1.4 ml) was added 4M aqueous sodium hydroxide solution (0.23 ml) at room temperature, and the mixture was stirred at 60Â° C. for 2 hr. To the reaction mixture were added 10% aqueous citric acid solution (0.070 ml) and water at room temperature, and the mixture was stirred. The precipitated solid was collected by filtration, washed with water, and dried under reduced pressure to give the title compound (0.070 g, yield 92%).

Production Example 11Synthesis of N-{4-chloro-3-[4-hydroxy-6-(4-isopropylphenyl)-1,3,5-triazin-2-yl]benzyl}-3,3,3-trifluoro-2,2-dimethylpropionamide (Example No. 1-98)

[Image Omitted]

(1) 2-chloro-4-(4-isopropylphenyl)-6-methoxy-1,3,5-triazine

[Image Omitted]

Under an argon atmosphere, to a suspension of 4-isopropylphenylboronic acid (0.30 g, 1.7 mmol), 2,4-dichloro-6-methoxy-1,3,5-triazine (0.23 g, 1.4 mmol) and [1,1â€²-bis(diphenylphosphino) ferrocene]palladium(II)dichloride dichloromethane adduct (0.11 g, 0.14 mmol) in 1,4-dioxane (4.0 ml) was added 2M aqueous sodium carbonate solution (2.0 ml) at room temperature, and the mixture was stirred at 100Â° C. for 1.5 hr. At room temperature, to the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=20/1) to give the title compound (0.21 g, yield 57%).

¹H-NMR (CDCl₃) Î´: 1.29 (6H, d, J=7.1 Hz), 2.99-3.02 (1H, m), 4.16 (3H, s), 7.34-7.38 (2H, m), 8.39-8.43 (2H, m).

(2) {4-chloro-3-[4-(4-isopropylphenyl)-6-methoxy-1,3,5-triazin-2-yl]phenyl}methanol

[Image Omitted]

Under an argon atmosphere, to a suspension of 2-chloro-4-(4-isopropylphenyl)-6-methoxy-1,3,5-triazine (0.21 g) obtained in the above-mentioned (1), 2-chloro-5-hydroxymethylphenylboronic acid (0.15 g, 0.80 mmol) and [1,1â€²-bis(diphenylphosphino)ferrocene]palladium(II)dichloride dichloromethane adduct (0.066 g, 0.080 mmol) in 1,4-dioxane (2.4 ml) was added 2M aqueous sodium carbonate solution (1.2 ml) at room temperature, and the mixture was stirred at 100Â° C. for 2 hr. At room temperature, to the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with water, washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=5/3) to give the title compound (0.15 g, yield 51%).

¹H-NMR (CDCl₃) Î´: 1.30 (6H, d, J=7.1 Hz), 1.77 (1H, t, J=6.1 Hz), 2.95-3.07 (1H, m), 4.20 (3H, s), 4.77 (2H, d, J=6.1 Hz), 7.35-7.39 (2H, m), 7.46 (1H, dd, J=8.2, 2.2 Hz), 7.54 (1H, d, J=8.2 Hz), 8.01 (1H, dd, J=2.2, 0.4 Hz), 8.50-8.54 (2H, m).

(3) tert-butyl N-{4-chloro-3-[4-(4-isopropylphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzyl}-N-(tert-butoxycarbonyl)carbamate

[Image Omitted]

Under an argon atmosphere, to a solution of {4-chloro-3-[4-(4-isopropylphenyl)-6-methoxy-1,3,5-triazin-2-yl]phenyl}methanol (0.15 g, 0.41 mmol) obtained in the above-mentioned (2) and triphenylphosphine (0.16 g, 0.62 mmol) in chloroform (1.5 ml) was added carbon tetrabromide (0.20 g, 0.62 mmol) under ice-cooling, and the mixture was stirred at room temperature for 10 min. The reaction mixture was applied to silica gel column chromatography (eluent: n-hexane/ethyl acetate=30/1âˆ’10/1) and concentrated under reduced pressure.

A solution of the residue in N,N-dimethylformamide (1.5 ml) was added to a solution of di-tert-butyl iminodicarboxylate (0.089 g, 0.41 mmol) and sodium hydride (0.016 g, 60 wt % oil dispersion) in N,N-dimethylformamide (0.70 ml) under ice-cooling, and the mixture was stirred at room temperature for 15 min. To the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with water, washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=5/1) to give the title compound (0.20 g, yield 85%).

¹H-NMR (CDCl₃) Î´: 1.30 (6H, d, J=7.0 Hz), 1.47 (18H, s), 2.94-3.05 (1H, m), 4.19 (3H, s), 4.83 (2H, s), 7.34-7.41 (3H, m), 7.48 (1H, d, J=8.4 Hz), 8.00 (1H, d, J=2.3 Hz), 8.49-8.53 (2H, m).

(4) 4-chloro-3-[4-(4-isopropylphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzylamine hydrochloride

[Image Omitted]

Under an argon atmosphere, to tert-butyl N-{4-chloro-3-[4-(4-isopropylphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzyl}-N-(tert-butoxycarbonyl)carbamate (0.20 g, 0.35 mmol) obtained in the above-mentioned (3) was added 4M hydrogen chloride/1,4-dioxane solution (2.0 ml) at room temperature, and the mixture was stirred for 1 hr. The suspension was concentrated under reduced pressure, and azeotropically distilled with ethyl acetate (twice) to give the title compound as a crude product (0.14 g).

(5) N-{4-chloro-3-[4-(4-isopropylphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzyl}-3,3,3-trifluoro-2,2-dimethylpropionamide

[Image Omitted]

Under an argon atmosphere, to a solution of a crude product (0.10 g) of 4-chloro-3-[4-(4-isopropylphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzylamine hydrochloride obtained in the above-mentioned (4), HOBt.H₂O (0.052 g, 0.34 mmol) and WSC.HCl (0.066 g, 0.34 mmol) in N,N-dimethylformamide (1.0 ml) were added 3,3,3-trifluoro-2,2-dimethylpropionic acid (0.042 g, 0.27 mmol) and triethylamine (0.069 ml, 0.49 mmol) at room temperature, and the mixture was stirred for 4 hr. To the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=1/1) to give the title compound (0.054 g).

¹H-NMR (CDCl₃) Î´: 1.30 (6H, d, J=6.8 Hz), 1.44 (6H, s), 2.95-3.05 (1H, m), 4.18 (3H, s), 4.53 (2H, d, J=5.7 Hz), 6.34 (1H, br s), 7.30-7.39 (3H, m), 7.50 (1H, d, J=8.4 Hz), 7.91 (1H, d, J=2.2 Hz), 8.49-8.53 (2H, m).

(6) N-{4-chloro-3-[4-hydroxy-6-(4-isopropylphenyl)-1,3,5-triazin-2-yl]benzyl}-3,3,3-trifluoro-2,2-dimethylpropionamide (Example No. 1-98)

[Image Omitted]

Under an argon atmosphere, to a solution of N-{4-chloro-3-[4-(4-isopropylphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzyl}-3,3,3-trifluoro-2,2-dimethylpropionamide (0.050 g, 0.099 mmol) obtained in the above-mentioned (5) in methanol (0.50 ml) was added 4M aqueous sodium hydroxide solution (0.20 ml) at room temperature, and the mixture was stirred at 60Â° C. for 2 hr. To the reaction mixture were added 2N hydrochloric acid (0.40 ml) and water at room temperature, and the mixture was stirred. The precipitated solid was collected by filtration, washed with water, and dried under reduced pressure to give the title compound (0.043 g, yield 89%).

Production Example 12Synthesis of N-{4-chloro-3-[4-hydroxy-6-(4-isobutoxyphenyl)-1,3,5-triazin-2-yl]benzyl}-3,3,3-trifluoro-2,2-dimethylpropionamide (Example No. 1-109)

[Image Omitted]

(1) 2-chloro-4-(4-isobutoxyphenyl)-6-methoxy-1,3,5-triazine

[Image Omitted]

Under an argon atmosphere, to a suspension of 4-isobutoxyphenylboronic acid (0.50 g, 2.58 mmol), 2,4-dichloro-6-methoxy-1,3,5-triazine (0.93 g, 5.15 mmol), tetrakis(triphenylphosphine)palladium(0) (0.15 g, 0.129 mmol) and sodium carbonate (0.819 g, 7.73 mmol) in toluene (5.0 ml) was added distilled water (3.5 ml), and the mixture was stirred at 86Â° C. for 2 hr. At room temperature, to the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=10/1) to give the title compound (0.606 g, yield 80%).

¹H-NMR (CDCl₃) Î´: 1.05 (6H, d, J=6.7 Hz), 2.07-2.17 (1H, m), 3.81 (2H, d, J=6.5 Hz), 4.14 (3H, s), 6.95-7.00 (2H, m), 8.42-8.46 (2H, m).

(2) {4-chloro-3-[4-(4-isobutoxyphenyl)-6-methoxy-1,3,5-triazin-2-yl]phenyl}methanol

[Image Omitted]

Under an argon atmosphere, a suspension of 2-chloro-4-(4-isobutoxyphenyl)-6-methoxy-1,3,5-triazine (0.60 g, 2.0 mmol) obtained in the above-mentioned (1), 2-chloro-5-hydroxymethylphenylboronic acid (0.57 g, 3.1 mmol), [1,1â€²-bis(diphenylphosphino)ferrocene]palladium(II)dichloride dichloromethane adduct (0.083 g, 0.10 mmol) and tripotassium phosphate (1.3 g, 6.1 mmol) in N,N-dimethylformamide (6.0 ml) was stirred at 60Â° C. for 1.5 hr. At room temperature, to the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with water and partitioned, washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=3/2) to give the title compound (0.32 g, yield 39%).

¹H-NMR (CDCl₃) Î´: 1.05 (6H, d, J=6.7 Hz), 1.77 (1H, t, J=5.9 Hz), 2.08-2.18 (1H, m), 3.82 (2H, d, J=6.5 Hz), 4.19 (3H, s), 4.77 (2H, d, J=5.9 Hz), 6.98-7.01 (2H, m), 7.46 (1H, dd, J=8.2, 2.2 Hz), 7.53 (1H, d, J=8.2 Hz), 8.00 (1H, d, J=2.2 Hz), 8.55 (2H, m).

(3) tert-butyl N-{4-chloro-3-[4-(4-isobutoxyphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzyl}-N-(tert-butoxycarbonyl)carbamate

[Image Omitted]

Under an argon atmosphere, to a solution of {4-chloro-3-[4-(4-isobutoxyphenyl)-6-methoxy-1,3,5-triazin-2-yl]phenyl}methanol (0.24 g, 0.61 mmol) obtained in the above-mentioned (2) in tetrahydrofuran (2.0 ml) were added triethylamine (0.11 ml, 0.79 mmol) and methanesulfonyl chloride (0.052 ml, 0.67 mmol) under ice-cooling, and the mixture was stirred for 0.5 hr. The reaction mixture was filtered, and the filtrate was concentrated under reduced pressure. To a solution of the residue in N,N-dimethylformamide (1.5 ml) were added cesium carbonate (0.59 g, 1.8 mmol) and di-tert-butyl iminodicarboxylate (0.16 g, 0.73 mmol) at room temperature, and the mixture was stirred for 1.5 hr. To the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated brine, dried over magnesium sulfate, filtered to remove magnesium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=6/1) to give the title compound (0.34 g, yield 92%).

¹H-NMR (CDCl₃) Î´: 1.05 (6H, d, J=6.7 Hz), 1.47 (18H, s), 2.08-2.18 (1H, m), 3.82 (2H, d, J=6.5 Hz), 4.18 (3H, s), 4.82 (2H, s), 6.96-7.00 (2H, m), 7.39 (1H, dd, J=8.3, 2.3 Hz), 7.48 (1H, d, J=8.3 Hz), 7.99 (1H, d, J=2.3 Hz), 8.52-8.56 (2H, m).

(4) 4-chloro-3-[4-(4-isobutoxyphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzylamine hydrochloride

[Image Omitted]

Under an argon atmosphere, to a solution of tert-butyl N-{4-chloro-3-[4-(4-isobutoxyphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzyl}-N-(tert-butoxycarbonyl)carbamate (0.34 g, 0.56 mmol) obtained in the above-mentioned (3) in 1,4-dioxane (1.0 ml) was added 4M hydrogen chloride/1,4-dioxane solution (3.0 ml) at room temperature, and the mixture was stirred for 2.5 hr. To the reaction mixture was added n-hexane (20 ml), and the mixture was stirred. The solid was collected by filtration from the suspension, and dried under reduced pressure to give the title compound (0.24 g, yield 95%).

¹H-NMR (DMSO-D₆) Î´: 1.01 (6H, d, J=6.8 Hz), 2.01-2.11 (1H, m), 3.88 (2H, d, J=6.4 Hz), 4.14 (3H, s), 4.12-4.17 (2H, m), 7.12-7.15 (2H, m), 7.72 (2H, br s), 8.13 (1H, br s), 8.40-8.51 (5H, m).

(5) N-{4-chloro-3-[4-(4-isobutoxyphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzyl}-3,3,3-trifluoro-2,2-dimethylpropionamide

[Image Omitted]

Under an argon atmosphere, to a solution of 4-chloro-3-[4-(4-isobutoxyphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzylamine hydrochloride (0.065 g, 0.14 mmol) obtained in the above-mentioned (4), HOBt.H₂O (0.033 g, 0.22 mmol) and 3,3,3-trifluoro-2,2-dimethylpropionic acid (0.034 g, 0.22 mmol) in N,N-dimethylformamide (0.70 ml) were added WSC.HCl (0.042 g, 0.22 mmol) and triethylamine (0.030 ml, 0.22 mmol) at room temperature, and the mixture was stirred for 5 hr. To the reaction mixture were added 3,3,3-trifluoro-2,2-dimethylpropionic acid (0.034 g, 0.22 mmol), WSC.HCl (0.042 g, 0.22 mmol), HOBt.H₂O (0.033 g, 0.22 mmol) and triethylamine (0.030 ml, 0.22 mmol), and the mixture was stirred for 1 hr. To the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated brine, dried over magnesium sulfate, filtered to remove magnesium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=3/1) to give the title compound (0.068 g, yield 86%).

¹H-NMR (CDCl₃) Î´: 1.06 (6H, d, J=6.8 Hz), 1.44 (6H, br s), 2.08-2.18 (1H, m), 3.82 (2H, d, J=6.6 Hz), 4.19 (3H, s), 4.55 (2H, d, J=5.7 Hz), 6.21 (1H, br s), 6.97-7.01 (2H, m), 7.34 (1H, dd, J=8.3, 2.3 Hz), 7.51 (1H, d, J=8.3 Hz), 7.91 (1H, d, J=2.3 Hz), 8.53-8.55 (2H, m).

(6) N-{4-chloro-3-[4-hydroxy-6-(4-isobutoxyphenyl)-1,3,5-triazin-2-yl]benzyl}-3,3,3-trifluoro-2,2-dimethylpropionamide (Example No. 1-109)

[Image Omitted]

Under an argon atmosphere, to a solution of N-{4-chloro-3-[4-(4-isobutoxyphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzyl}-3,3,3-trifluoro-2,2-dimethylpropionamide (0.066 g, 0.12 mmol) obtained in the above-mentioned (5) in methanol (1.1 ml) was added 4M aqueous sodium hydroxide solution (0.18 ml) at room temperature, and the mixture was stirred at 60Â° C. for 2 hr. To the reaction mixture were added 10% aqueous citric acid solution (0.55 ml) and water at room temperature, and the mixture was stirred. The precipitated solid was collected by filtration, washed with water, and dried under reduced pressure to give the title compound (0.057 g, yield 88%). A suspension of the title compound (0.030 g) in acetonitrile (0.60 ml) was stirred at room temperature, and the solid was collected by filtration and dried to give the title compound as crystals (0.011 g).

Production Example 13Synthesis of N-{4-chloro-3-[4-hydroxy-6-(4-propoxyphenyl)-1,3,5-triazin-2-yl]benzyl}-3,3,3-trifluoro-2,2-dimethylpropionamide (Example No. 1-122)

[Image Omitted]

(1) 2-chloro-4-methoxy-6-(4-propoxyphenyl)-1,3,5-triazine

[Image Omitted]

Under an argon atmosphere, to a suspension of 4-propoxyphenylboronic acid (1.0 g, 5.6 mmol), 2,4-dichloro-6-methoxy-1,3,5-triazine (2.0 g, 11 mmol) and tetrakis(triphenylphosphine)palladium(0) (0.65 g, 0.56 mmol) in toluene (25 ml) was added 2M aqueous sodium carbonate solution (8.4 ml), and the mixture was stirred at 100Â° C. for 1 hr. At room temperature, to the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated brine, dried over magnesium sulfate, filtered to remove magnesium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=20/1) to give the title compound (1.1 g, yield 70%).

¹H-NMR (CDCl₃) Î´: 1.06 (3H, t, J=7.4 Hz), 1.83-1.87 (2H, m), 4.02 (2H, t, J=6.6 Hz), 4.14 (3H, s), 6.96-6.99 (2H, m), 8.43-8.45 (2H, m).

(2) {4-chloro-3-[4-methoxy-6-(4-propoxyphenyl)-1,3,5-triazin-2-yl]phenyl}methanol

[Image Omitted]

Under an argon atmosphere, to a solution of 2-chloro-4-methoxy-6-(4-propoxyphenyl)-1,3,5-triazine (0.75 g, 2.7 mmol) obtained in the above-mentioned (1), 2-chloro-5-hydroxymethylphenylboronic acid (0.60 g, 3.2 mmol) and [1,1â€²-bis(diphenylphosphino)ferrocene]palladium(II)dichloride dichloromethane adduct (0.22 g, 0.27 mmol) in 1,4-dioxane (15 ml) was added 2M aqueous sodium carbonate solution (5.4 ml), and the mixture was stirred at 100Â° C. for 3 hr. At room temperature, to the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with water and partitioned, washed with saturated brine, dried over magnesium sulfate, filtered to remove magnesium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: chloroform/ethyl acetate=10/1) to give the title compound (0.95 g, yield 91%).

¹H-NMR (CDCl₃) Î´: 1.07 (3H, t, J=7.4 Hz), 1.77 (1H, t, J=5.8 Hz), 1.84-1.87 (2H, m), 4.02 (2H, t, J=6.6 Hz), 4.19 (3H, s), 4.77 (2H, d, J=5.8 Hz), 7.00 (2H, d, J=8.7 Hz), 7.45 (1H, dd, J=8.3, 1.9 Hz), 7.53 (1H, d, J=8.3 Hz), 8.00 (1H, d, J=1.9 Hz), 8.55 (2H, d, J=8.7 Hz).

(3) tert-butyl N-{4-chloro-3-[4-methoxy-6-(4-propoxyphenyl)-1,3,5-triazin-2-yl]benzyl}-N-(tert-butoxycarbonyl)carbamate

[Image Omitted]

Under an argon atmosphere, to a solution of {4-chloro-3-[4-methoxy-6-(4-propoxyphenyl)-1,3,5-triazin-2-yl]phenyl}methanol (0.95 g, 2.5 mmol) obtained in the above-mentioned (2) in tetrahydrofuran (13 ml) were added triethylamine (0.45 ml, 3.2 mmol) and methanesulfonyl chloride (0.23 ml, 3.0 mmol) under ice-cooling, and the mixture was stirred for 0.5 hr. The reaction mixture was filtered, and the filtrate was concentrated under reduced pressure. To a solution of the residue in N,N-dimethylformamide (13 ml) were added cesium carbonate (2.4 g, 7.4 mmol) and di-tert-butyl iminodicarboxylate (0.64 g, 3.0 mmol) at room temperature, and the mixture was stirred for 1 hr. To the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated brine, dried over magnesium sulfate, filtered to remove magnesium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=10/1) to give the title compound (1.3 g, yield 90%).

(4) 4-chloro-3-[4-methoxy-6-(4-propoxyphenyl)-1,3,5-triazin-2-yl]benzylamine hydrochloride

[Image Omitted]

Under an argon atmosphere, to tert-butyl N-{4-chloro-3-[4-methoxy-6-(4-propoxyphenyl)-1,3,5-triazin-2-yl]benzyl}-N-(tert-butoxycarbonyl)carbamate(1.3 g, 2.2 mmol) obtained in the above-mentioned (3) was added 4M hydrogen chloride/1,4-dioxane solution (5.0 ml) at room temperature, and the mixture was stirred for 30 min. To the reaction mixture were added 1,4-dioxane (2.0 ml) and n-hexane (5.0 ml), and the mixture was stirred for 45 min. The solid was collected by filtration from the suspension, and dried under reduced pressure to give the title compound (0.68 g, yield 73%).

¹H-NMR (DMSO-D₆) Î´: 1.00 (3H, t, J=7.4 Hz), 1.73-1.83 (2H, m), 4.06 (2H, t, J=6.5 Hz), 4.12-4.18 (2H, m), 4.14 (3H, s), 7.12-7.16 (2H, m), 7.69-7.74 (2H, m), 8.13 (1H, br s), 8.44 (3H, br s), 8.45-8.50 (2H, m).

(5) N-{4-chloro-3-[4-methoxy-6-(4-propoxyphenyl)-1,3,5-triazin-2-yl]benzyl}-3,3,3-trifluoro-2,2-dimethylpropionamide

[Image Omitted]

Under an argon atmosphere, to a solution of 4-chloro-3-[4-methoxy-6-(4-propoxyphenyl)-1,3,5-triazin-2-yl]benzylamine hydrochloride (0.10 g, 0.24 mmol) obtained in the above-mentioned (4), HOBt.H₂O (0.054 g, 0.36 mmol) and WSC.HCl (0.068 g, 0.36 mmol) in N,N-dimethylformamide (1.5 ml) were added 3,3,3-trifluoro-2,2-dimethylpropionic acid (0.056 g, 0.36 mmol) and triethylamine (0.099 ml, 0.71 mmol) at room temperature, and the mixture was stirred for 4 hr. To the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated brine, dried over magnesium sulfate, filtered to remove magnesium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=10/3) to give the title compound (0.096 g, yield 78%).

¹H-NMR (DMSO-D₆) Î´: 1.00 (3H, t, J=7.3 Hz), 1.39 (6H, s), 1.73-1.83 (2H, m), 4.05 (2H, t, J=6.5 Hz), 4.11 (3H, s), 4.39 (2H, d, J=5.9 Hz), 7.10-7.14 (2H, m), 7.44 (1H, dd, J=8.1, 2.3 Hz), 7.61 (1H, d, J=8.1 Hz), 7.86 (1H, d, J=2.3 Hz), 8.42-8.47 (2H, m), 8.66 (1H, t, J=5.9 Hz).

(6) N-{4-chloro-3-[4-hydroxy-6-(4-propoxyphenyl)-1,3,5-triazin-2-yl]benzyl}-3,3,3-trifluoro-2,2-dimethylpropionamide (Example No. 1-122)

[Image Omitted]

Under an argon atmosphere, to a solution of N-{4-chloro-3-[4-methoxy-6-(4-propoxyphenyl)-1,3,5-triazin-2-yl]benzyl}-3,3,3-trifluoro-2,2-dimethylpropionamide (0.094 g, 0.18 mmol) obtained in the above-mentioned (5) in methanol (0.94 ml) was added 4M aqueous sodium hydroxide solution (0.27 ml) at room temperature, and the mixture was stirred at 65Â° C. for 2 hr. To the reaction mixture were added 2N hydrochloric acid (0.54 ml) and water at room temperature, and the mixture was stirred. The precipitated solid was collected by filtration, washed with water, and dried under reduced pressure to give the title compound (0.069 g, yield 75%). A suspension of the title compound (0.050 g) in acetone (1.0 ml) was dissolved by heating under reflux, and the solid was collected by filtration at room temperature and dried to give the title compound as crystals (0.012 g).

Production Example 14Synthesis of N-(4-chloro-3-{4-hydroxy-6-[4-(1-methylcyclopropylmethoxy)phenyl]-1,3,5-triazin-2-yl}benzyl)-3,3,3-trifluoro-2,2-dimethylpropionamide (Example No. 1-128)

[Image Omitted]

(1) 2-chloro-4-methoxy-6-(4-methoxymethoxyphenyl)-1,3,5-triazine

[Image Omitted]

Under an argon atmosphere, to a suspension of 4-(methoxymethoxy)phenylboronic acid (1.0 g, 5.5 mmol), 2,4-dichloro-6-methoxy-1,3,5-triazine (2.0 g, 11 mmol) and tetrakis(triphenylphosphine)palladium(0) (0.64 g, 0.55 mmol) in toluene (25 ml) was added 2M aqueous sodium carbonate solution (8.3 ml), and the mixture was stirred at 100Â° C. for 1 hr. At room temperature, to the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated brine, dried over magnesium sulfate, filtered to remove magnesium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=20/1âˆ’10/1) to give the title compound (1.3 g, yield 84%).

(2) {4-chloro-3-[4-methoxy-6-(4-methoxymethoxyphenyl)-1,3,5-triazin-2-yl]phenyl}methanol

[Image Omitted]

Under an argon atmosphere, to a solution of 2-chloro-4-methoxy-6-(4-methoxymethoxyphenyl)-1,3,5-triazine (1.3 g, 4.4 mmol) obtained in the above-mentioned (1), 2-chloro-5-hydroxymethylphenylboronic acid (0.99 g, 5.3 mmol) and [1,1â€²-bis(diphenylphosphino)ferrocene]palladium(II)dichloride dichloromethane adduct (0.36 g, 0.44 mmol) in 1,4-dioxane (25 ml) was added 2M aqueous sodium carbonate solution (8.8 ml), and the mixture was stirred at 100Â° C. for 3 hr. At room temperature, to the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with water, washed with saturated brine, dried over magnesium sulfate, filtered to remove magnesium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: chloroform/ethyl acetate=10/1) to give the title compound (0.98 g, yield 56%).

(3) tert-butyl N-{4-chloro-3-[4-methoxy-6-(4-methoxymethoxyphenyl)-1,3,5-triazin-2-yl]benzyl}-N-(tert-butoxycarbonyl)carbamate

[Image Omitted]

Under an argon atmosphere, to a solution of {4-chloro-3-[4-methoxy-6-(4-methoxymethoxyphenyl)-1,3,5-triazin-2-yl]phenyl}methanol (0.78 g, 2.0 mmol) obtained in the above-mentioned (2) in tetrahydrofuran (7.8 ml) were added triethylamine (0.36 ml, 2.6 mmol) and methanesulfonyl chloride (0.19 ml, 2.4 mmol) under ice-cooling, and the mixture was stirred for 0.5 hr. The reaction mixture was filtered, and the filtrate was concentrated under reduced pressure. To a solution of the residue in N,N-dimethylformamide (10 ml) were added cesium carbonate (2.0 g, 6.0 mmol) and di-tert-butyl iminodicarboxylate (0.53 g, 2.4 mmol), and the mixture was stirred for 2 hr. To the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated brine, dried over magnesium sulfate, filtered to remove magnesium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=10/1) to give the title compound (0.80 g, yield 68%).

¹H-NMR (DMSO-D₆) Î´: 1.42 (18H, s), 3.41 (3H, s), 4.12 (3H, s), 4.77 (2H, s), 5.32 (2H, s), 7.18-7.23 (2H, m), 7.45 (1H, dd, J=8.2, 2.3 Hz), 7.65 (1H, d, J=8.2 Hz), 7.91 (1H, d, J=2.3 Hz), 8.43-8.47 (2H, m).

(4) 4-[4-(5-aminomethyl-2-chlorophenyl)-6-methoxy-1,3,5-triazin-2-yl]phenol hydrochloride

[Image Omitted]

Under an argon atmosphere, to tert-butyl N-{4-chloro-3-[4-methoxy-6-(4-methoxymethoxyphenyl)-1,3,5-triazin-2-yl]benzyl}-N-(tert-butoxycarbonyl)carbamate (0.40 g, 0.68 mmol) obtained in the above-mentioned (3) was added 4M hydrogen chloride/1,4-dioxane solution (2.0 ml) at room temperature, and the mixture was stirred for 1 hr. To the reaction mixture was added n-hexane (3.0 ml), and the mixture was stirred for 45 min. The solid was collected by filtration, and dried under reduced pressure to give the title compound as a crude product (0.26 g).

(5) 4-(4-{2-chloro-5-[(3,3,3-trifluoro-2,2-dimethylpropionylamino)methyl]phenyl}-6-methoxy-1,3,5-triazin-2-yl)phenyl 3,3,3-trifluoro-2,2-dimethylpropionate

[Image Omitted]

Under an argon atmosphere, to a solution of a crude product (0.10 g) of 4-[4-(5-aminomethyl-2-chlorophenyl)-6-methoxy-1,3,5-triazin-2-yl]phenol hydrochloride obtained in the above-mentioned (4), HOBt.H₂O (0.061 g, 0.40 mmol) and WSC.HCl (0.076 g, 0.40 mmol) in N,N-dimethylformamide (1.5 ml) were added 3,3,3-trifluoro-2,2-dimethylpropionic acid (0.066 g, 0.40 mmol) and triethylamine (0.11 ml, 0.79 mmol) at room temperature, and the mixture was stirred for 2 hr. To the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated brine, dried over magnesium sulfate, filtered to remove magnesium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: chloroform/ethyl acetate=10/1) to give the title compound (0.090 g).

¹H-NMR (DMSO-D₆) Î´: 1.39 (6H, s), 1.59 (6H, s), 4.15 (3H, s), 4.40 (2H, d, J=6.0 Hz), 7.39-7.47 (3H, m), 7.62 (1H, d, J=8.4 Hz), 7.88 (1H, d, J=2.1 Hz), 8.55-8.60 (2H, m), 8.66 (1H, t, J=6.0 Hz).

(6) N-{4-chloro-3-[4-(4-hydroxyphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzyl}-3,3,3-trifluoro-2,2-dimethylpropionamide

[Image Omitted]

Under an argon atmosphere, to a solution of 4-(4-{2-chloro-5-[(3,3,3-trifluoro-2,2-dimethylpropionylamino)methyl]phenyl}-6-methoxy-1,3,5-triazin-2-yl)phenyl 3,3,3-trifluoro-2,2-dimethylpropionate (0.070 g, 0.15 mmol) obtained in the above-mentioned (5) in methanol (0.70 ml) was added 5M sodium methoxide/methanol solution (0.032 ml) at room temperature, and the mixture was stirred for 1 hr. The reaction mixture was adjusted to pH=2 with 2N hydrochloric acid under ice-cooling. To the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated brine, dried over magnesium sulfate, filtered to remove magnesium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: chloroform/ethyl acetate=4/1) to give the title compound (0.036 g, yield 51%).

¹H-NMR (DMSO-D₆) Î´: 1.38 (6H, s), 4.10 (3H, s), 4.39 (2H, d, J=6.2 Hz), 6.91-6.95 (2H, m), 7.42 (1H, dd, J=8.3, 2.3 Hz), 7.60 (1H, d, J=8.3 Hz), 7.84 (1H, d, J=2.3 Hz), 8.34-8.39 (2H, m), 8.65 (1H, t, J=6.2 Hz), 10.38 (1H, br s).

(7) N-(4-chloro-3-{4-methoxy-6-[4-(1-methylcyclopropylmethoxyl)phenyl]-1,3,5-triazin-2-yl}benzyl)-3,3,3-trifluoro-2,2-dimethylpropionamide

[Image Omitted]

Under an argon atmosphere, to a solution of N-{4-chloro-3-[4-(4-hydroxyphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzyl}-3,3,3-trifluoro-2,2-dimethylpropionamide (0.036 g, 0.075 mmol) obtained in the above-mentioned (6), 1-methyl-cyclopropanemethanol (0.0087 ml, 0.090 mmol) and triphenylphosphine (0.024 g, 0.090 mmol) in tetrahydrofuran (0.50 ml) was added 1.9M diethyl azodicarboxylate/toluene solution (0.051 ml, 0.098 mmol) under ice-cooling, and the mixture was stirred for 1 hr. The reaction mixture was stirred at room temperature for 1 hr, and 1.9M diethyl azodicarboxylate/toluene solution (0.028 ml, 0.053 mmol) was added. The reaction mixture was stirred at room temperature for 1 hr, and purified by preparative thin layer chromatography (eluent: chloroform/ethyl acetate=19/1) to give the title compound (0.029 g, yield 70%).

¹H-NMR (DMSO-D₆) Î´: 0.42 (2H, dd, J=5.6, 4.0 Hz), 0.56 (2H, dd, J=5.4, 4.2 Hz), 1.20 (3H, s), 1.39 (6H, s), 3.88 (2H, s), 4.11 (3H, s), 4.39 (2H, d, J=5.9 Hz), 7.09-7.14 (2H, m), 7.43 (1H, dd, J=8.2, 2.1 Hz), 7.60 (1H, d, J=8.2 Hz), 7.85 (1H, d, J=2.1 Hz), 8.41-8.46 (2H, m), 8.66 (1H, t, J=5.9 Hz).

(8) N-(4-chloro-3-{4-hydroxy-6-[4-(1-methylcyclopropylmethoxyl)phenyl]-1,3,5-triazin-2-yl}benzyl)-3,3,3-trifluoro-2,2-dimethylpropionamide (Example No. 1-128)

[Image Omitted]

Under an argon atmosphere, to a solution of N-(4-chloro-3-{4-methoxy-6-[4-(1-methylcyclopropylmethoxyl)phenyl]-1,3,5-triazin-2-yl}benzyl)-3,3,3-trifluoro-2,2-dimethylpropionamide (0.028 g, 0.051 mmol) obtained in the above-mentioned (7) in methanol (0.28 ml) was added 4M aqueous sodium hydroxide solution (0.077 ml) at room temperature, and the mixture was stirred at 60Â° C. for 1 hr. To the reaction mixture were added 2N hydrochloric acid (0.16 ml) and water at room temperature, and the mixture was stirred. The precipitated solid was collected by filtration, washed with water, and dried under reduced pressure to give the title compound (0.019 g, yield 69%).

Production Example 15Synthesis of N-{4-chloro-3-[4-(3-chloro-4-methylphenyl)-6-hydroxy-1,3,5-triazin-2-yl]benzyl}-3,3,3-trifluoro-2,2-dimethylpropionamide (Example No. 1-129)

[Image Omitted]

(1) 2-chloro-4-(3-chloro-4-methylphenyl)-6-methoxy-1,3,5-triazine

[Image Omitted]

Under an argon atmosphere, to a suspension of 3-chloro-4-methylphenylboronic acid (0.47 g, 2.8 mmol), 2,4-dichloro-6-methoxy-1,3,5-triazine (1.0 g, 5.6 mmol) and tetrakis(triphenylphosphine)palladium(0) (0.32 g, 0.28 mmol) in toluene (5.0 ml) was added 2M aqueous sodium carbonate solution (4.2 ml), and the mixture was stirred at 100Â° C. for 2 hr. At room temperature, to the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=97/3âˆ’94/6) to give the title compound (0.61 g, yield 81%).

¹H-NMR (CDCl₃) Î´: 2.47 (3H, s), 4.17 (3H, s), 7.37 (1H, d, J=8.0 Hz), 8.28 (1H, dd, J=8.0, 1.8 Hz), 8.47 (1H, d, J=1.8 Hz).

(2){4-chloro-3-[4-(3-chloro-4-methylphenyl)-6-methoxy-1,3,5-triazin-2-yl]phenyl}methanol

[Image Omitted]

Under an argon atmosphere, to a solution of 2-chloro-4-(3-chloro-4-methylphenyl)-6-methoxy-1,3,5-triazine (0.61 g, 2.3 mmol) obtained in the above-mentioned (1), 2-chloro-5-hydroxymethylphenylboronic acid (0.51 g, 2.7 mmol) and [1,1â€²-bis(diphenylphosphino) ferrocene]palladium(II)dichloride dichloromethane adduct (0.19 g, 0.23 mmol) in 1,4-dioxane (6.0 ml) was added 2M aqueous sodium carbonate solution (4.5 ml), and the mixture was stirred at 100Â° C. for 1.5 hr. At room temperature, to the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=8/2âˆ’6/4) to give the title compound (0.61 g, yield 71%).

¹H-NMR (CDCl₃) Î´: 1.81 (1H, t, J=5.9 Hz), 2.47 (3H, s), 4.21 (3H, s), 4.78 (2H, d, J=5.9 Hz), 7.37 (1H, d, J=7.9 Hz), 7.47 (1H, dd, J=8.1, 2.2 Hz), 7.54 (1H, d, J=8.1 Hz), 8.01 (1H, d, J=2.2 Hz), 8.38 (1H, dd, J=7.9, 1.8 Hz), 8.57 (1H, d, J=1.8 Hz).

(3) 4-chloro-3-[4-(3-chloro-4-methylphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzylamine hydrochloride

[Image Omitted]

Under an argon atmosphere, to a solution of {4-chloro-3-[4-(3-chloro-4-methylphenyl)-6-methoxy-1,3,5-triazin-2-yl]phenyl}methanol (0.61 g, 1.6 mmol) obtained in the above-mentioned (2) in tetrahydrofuran (6.0 ml) were added triethylamine (0.29 ml, 2.1 mmol) and methanesulfonyl chloride (0.15 ml, 1.9 mmol) under ice-cooling, and the mixture was stirred for 1 hr. The reaction mixture was filtered, and the filtrate was concentrated under reduced pressure. To a solution of the residue in N,N-dimethylformamide (6.0 ml) were added cesium carbonate (1.6 g, 4.8 mmol) and di-tert-butyl iminodicarboxylate (0.42 g, 1.9 mmol) at room temperature, and the mixture was stirred for 2 hr. To the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=95/5âˆ’80/20). Under an argon atmosphere, to a solution of the purified product in 1,4-dioxane (2.0 ml) was added 4M hydrogen chloride/1,4-dioxane solution (8.0 ml) at room temperature, and the mixture was stirred for 2.5 hr. To the reaction mixture was added n-hexane, and the solid was collected by filtration, and dried under reduced pressure to give the title compound (0.67 g, yield 99%).

¹H-NMR (DMSO-D₆) Î´: 2.46 (3H, s), 4.12-4.21 (5H, m), 7.62 (1H, d, J=8.0 Hz), 7.73-7.75 (2H, m), 8.17 (1H, br s), 8.38 (1H, dd, J=8.0, 1.6 Hz), 8.47 (1H, d, J=1.6 Hz), 8.48 (3H, br s).

(4) N-{4-chloro-3-[4-(3-chloro-4-methylphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzyl}-3,3,3-trifluoro-2,2-dimethylpropionamide

[Image Omitted]

Under an argon atmosphere, to a solution of 4-chloro-3-[4-(3-chloro-4-methylphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzylamine hydrochloride (0.070 g, 0.17 mmol) obtained in the above-mentioned (3), HOBt.H₂O (0.039 g, 0.26 mmol) and WSC.HCl (0.049 g, 0.26 mmol) in N,N-dimethylformamide (0.70 ml) were added 3,3,3-trifluoro-2,2-dimethylpropionic acid (0.037 g, 0.24 mmol) and triethylamine (0.071 ml, 0.51 mmol) at room temperature, and the mixture was stirred for 1 hr. To the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=9/1âˆ’8/2) to give the title compound (0.072 g, yield 82%).

¹H-NMR (CDCl₃) Î´: 1.45 (6H, s), 2.47 (3H, s), 4.21 (3H, s), 4.56 (2H, d, J=5.6 Hz), 6.24 (1H, br s), 7.34-7.39 (2H, m), 7.52 (1H, d, J=8.2 Hz), 7.92 (1H, d, J=2.3 Hz), 8.38 (1H, dd, J=8.2, 1.8 Hz), 8.56 (1H, d, J=1.8 Hz).

(5) N-{4-chloro-3-[4-(3-chloro-4-methylphenyl)-6-hydroxy-1,3,5-triazin-2-yl]benzyl}-3,3,3-trifluoro-2,2-dimethylpropionamide (Example No. 1-129)

[Image Omitted]

Under an argon atmosphere, to a solution of N-{4-chloro-3-[4-(3-chloro-4-methylphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzyl}-3,3,3-trifluoro-2,2-dimethylpropionamide (0.072 g, 0.14 mmol) obtained in the above-mentioned (4) in methanol (0.70 ml) was added 4M aqueous sodium hydroxide solution (0.28 ml) at room temperature, and the mixture was stirred at 60Â° C. for 1 hr. To the reaction mixture were added 2N hydrochloric acid (0.56 ml) and water at room temperature, and the mixture was stirred. The precipitated solid was collected by filtration, washed with water, and dried under reduced pressure to give the title compound (0.057 g, yield 82%).

Production Example 16Synthesis of N-{4-chloro-3-[4-hydroxy-6-(3-isopropyl-4-trifluoromethylphenyl)-1,3,5-triazin-2-yl]benzyl}-3,3,3-trifluoro-2,2-dimethylpropionamide (Example No. 1-130)

[Image Omitted]

(1) 4-benzyloxy-2-bromo-1-trifluoromethylbenzene

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Under an argon atmosphere, to a solution of 2-bromo-4-fluoro-1-trifluoromethylbenzene (1.5 g, 6.2 mmol) and sodium hydride (0.74 g, 60 wt % oil dispersion) in N,N-dimethylformamide (15 ml) was added benzyl alcohol (0.64 ml, 6.2 mmol) under ice-cooling, and the mixture was stirred for 0.5 hr. The reaction mixture was stirred at 60Â° C. for 2 hr. At room temperature, to the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=99/1âˆ’98/2) to give the title compound (1.3 g, yield 69%).

¹H-NMR (CDCl₃) Î´: 5.08 (2H, s), 6.93 (1H, dd, J=8.8, 2.4 Hz), 7.30 (1H, d, J=2.4 Hz), 7.33-7.41 (5H, m), 7.57 (1H, d, J=8.8 Hz).

(2) 4-benzyloxy-2-isopropenyl-1-trifluoromethylbenzene

[Image Omitted]

Under an argon atmosphere, to a solution of 4-benzyloxy-2-bromo-1-trifluoromethylbenzene (1.3 g, 3.9 mmol) obtained in the above-mentioned (1) in 1,4-dioxane (13 ml) were added 2-isopropenyl-4,4,5,5-tetramethyl-[1,3,2]dioxaborolane (0.99 g, 5.9 mmol), [1,1â€²-bis(diphenylphosphino)ferrocene]palladium(II)dichloride dichloromethane adduct (0.32 g, 0.39 mmol) and 2M aqueous sodium carbonate solution (5.9 ml) at room temperature, and the mixture was stirred at 100Â° C. for 2 hr. At room temperature, to the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=99/1âˆ’97/3) to give the title compound (1.1 g, yield 99%).

¹H-NMR (CDCl₃) Î´: 2.04 (3H, s), 4.88 (1H, br s), 5.08 (2H, s), 5.18 (1H, br s), 6.82 (1H, d, J=2.6 Hz), 6.89 (1H, dd, J=8.8, 2.6 Hz), 7.31-7.42 (5H, m), 7.54 (1H, d, J=8.8 Hz).

(3) 3-isopropyl-4-trifluoromethylphenol

[Image Omitted]

Under an argon atmosphere, to a solution of 4-benzyloxy-2-isopropenyl-1-trifluoromethylbenzene (1.2 g, 3.9 mmol) obtained in the above-mentioned (2) in tetrahydrofuran (12 ml) was added 10 wt % palladium carbon (0.23 g) at room temperature, and the mixture was stirred under 1 atm hydrogen atmosphere for 5 hr. Under a nitrogen atmosphere, the reaction mixture was filtered through celite and eluted with ethyl acetate. The filtrate was concentrated under reduced pressure to give the title compound (0.76 g, yield 96%).

¹H-NMR (CDCl₃) Î´: 1.23 (6H, d, J=6.7 Hz), 3.24-3.35 (1H, m), 5.04 (1H, br s), 6.66 (1H, dd, J=8.6, 2.6 Hz), 6.87 (1H, d, J=2.6 Hz), 7.46 (1H, d, J=8.6 Hz).

(4) 3-isopropyl-4-trifluoromethylphenyl trifluoromethanesulfonate

[Image Omitted]

Under an argon atmosphere, to a solution of 3-isopropyl-4-trifluoromethylphenol (0.77 g, 3.8 mmol) obtained in the above-mentioned (3) in chloroform (8.0 ml) were added triethylamine (0.58 ml, 4.1 mmol) and trifluoromethanesulfonic anhydride (0.67 ml, 4.0 mmol) under ice-cooling, and the mixture was stirred for 1 hr. To the reaction mixture were added water and chloroform, and the mixture was partitioned. The organic layer was washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=98/2) to give the title compound (0.78 g, yield 62%).

¹H-NMR (CDCl₃) Î´: 1.28 (6H, d, J=6.7 Hz), 3.34-3.46 (1H, m), 7.19 (1H, dd, J=8.8, 2.4 Hz), 7.34 (1H, d, J=2.4 Hz), 7.70 (1H, d, J=8.8 Hz).

(5) 2-(3-isopropyl-4-trifluoromethylphenyl)-4,4,5,5-tetramethyl-[1,3,2]dioxaborolane

[Image Omitted]

Under an argon atmosphere, to a solution of 3-isopropyl-4-trifluoromethylphenyl trifluoromethanesulfonate (0.78 g, 2.3 mmol) obtained in the above-mentioned (4) in DMSO (8.0 ml) were added bis(pinacolato)diboron (0.71 g, 2.8 mmol), potassium acetate (0.68 g, 7.0 mmol) and [1,1â€²-bis(diphenylphosphino)ferrocene]palladium(II)dichloride dichloromethane adduct (0.095 g, 0.12 mmol) at room temperature, and the mixture was stirred at 80Â° C. for 2 hr. At room temperature, to the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=98/2) to give the title compound (0.48 g, yield 66%).

¹H-NMR (CDCl₃) Î´: 1.29 (6H, d, J=7.0 Hz), 1.36 (12H, s), 3.29-3.40 (1H, m), 7.57 (1H, d, J=7.9 Hz), 7.68 (1H, d, J=7.9 Hz), 7.88 (1H, br s).

(6) 2-chloro-4-(3-isopropyl-4-trifluoromethylphenyl)-6-methoxy-1,3,5-triazine

[Image Omitted]

Under an argon atmosphere, to a suspension of 2-(3-isopropyl-4-trifluoromethylphenyl)-4,4,5,5-tetramethyl-[1,3,2]dioxaborolane (0.48 g, 1.5 mmol) obtained in the above-mentioned (5), 2,4-dichloro-6-methoxy-1,3,5-triazine (0.69 g, 3.8 mmol) and [1,1â€²-bis (diphenylphosphino) ferrocene]palladium(II) dichloride dichloromethane adduct (0.13 g, 0.15 mmol) in 1,4-dioxane (5.0 ml) was added 2M aqueous sodium carbonate solution (3.1 mL), and the mixture was stirred at 100Â° C. for 1 hr. At room temperature, to the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=97/3âˆ’94/6) to give the title compound (0.36 g, yield 71%).

(7) {4-chloro-3-[4-(3-isopropyl-4-trifluoromethylphenyl)-6-methoxy-1,3,5-triazin-2-yl]phenyl}methanol

[Image Omitted]

Under an argon atmosphere, to a solution of 2-chloro-4-(3-isopropyl-4-trifluoromethylphenyl)-6-methoxy-1,3,5-triazine (0.36 g, 1.1 mmol) obtained in the above-mentioned (6), 2-chloro-5-hydroxymethylphenylboronic acid (0.25 g, 1.3 mmol) and [1,1â€²-bis(diphenylphosphino)ferrocene]palladium(II)dichloride dichloromethane adduct (0.089 g, 0.11 mmol) in 1,4-dioxane (3.6 ml) was added 2M aqueous sodium carbonate solution (2.2 ml), and the mixture was stirred at 100Â° C. for 1.5 hr. At room temperature, to the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=8/2âˆ’1/1) to give the title compound (0.30 g, yield 62%).

¹H-NMR (CDCl₃) Î´: 1.36 (6H, d, J=6.8 Hz), 1.79 (1H, t, J=6.0 Hz), 3.37-3.48 (1H, m), 4.24 (3H, s), 4.79 (2H, d, J=6.0 Hz), 7.49 (1H, dd, J=8.4, 2.2 Hz), 7.57 (1H, d, J=8.4 Hz), 7.75 (1H, d, J=8.4 Hz), 8.07 (1H, d, J=2.2 Hz), 8.47 (1H, d, J=8.4 Hz), 8.73 (1H, br s).

(8) 4-chloro-3-[4-(3-isopropyl-4-trifluoromethylphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzylamine hydrochloride

[Image Omitted]

Under an argon atmosphere, to a solution of {4-chloro-3-[4-(3-isopropyl-4-trifluoromethylphenyl)-6-methoxy-1,3,5-triazin-2-yl]phenyl}methanol (0.30 g, 0.68 mmol) obtained in the above-mentioned (7) in tetrahydrofuran (3.0 ml) were added triethylamine (0.12 ml, 0.89 mmol) and methanesulfonyl chloride (0.063 ml, 0.82 mmol) under ice-cooling, and the mixture was stirred for 0.5 hr. The reaction mixture was filtered, and the filtrate was concentrated under reduced pressure. To a solution of the residue in N,N-dimethylformamide (3.0 ml) were added cesium carbonate (0.67 g, 2.0 mmol) and di-tert-butyl iminodicarboxylate (0.18 g, 0.82 mmol) at room temperature, and the mixture was stirred for 1.5 hr. To the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=95/5âˆ’80/20). Under an argon atmosphere, to a solution (1.0 ml) of the purified product in 1,4-dioxane was added 4M hydrogen chloride/1,4-dioxane solution (4.0 ml) at room temperature, and the mixture was stirred for 1.5 hr. To the reaction mixture was added n-hexane, and the solid was collected by filtration and dried under reduced pressure to give the title compound (0.24 g, yield 74%).

¹H-NMR (DMSO-D₆) Î´: 1.33 (6H, d, J=6.7 Hz), 3.28-3.40 (1H, m), 4.13-4.22 (5H, m), 7.73 (1H, dd, J=8.2, 2.2 Hz), 7.77 (1H, d, J=8.3 Hz), 7.92 (1H, d, J=8.3 Hz), 8.20 (1H, d, J=2.2 Hz), 8.35 (3H, br s), 8.48 (1H, d, J=8.8 Hz), 8.70 (1H, s).

(9) N-{4-chloro-3-[4-(3-isopropyl-4-trifluoromethylphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzyl}-3,3,3-trifluoro-2,2-dimethylpropionamide

[Image Omitted]

Under an argon atmosphere, to a solution of 4-chloro-3-[4-(3-isopropyl-4-trifluoromethylphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzylamine hydrochloride (0.080 g, 0.17 mmol) obtained in the above-mentioned (8), HOBt.H₂O (0.039 g, 0.26 mmol) and WSC.HCl (0.049 g, 0.26 mmol) in N,N-dimethylformamide (0.80 ml) were added 3,3,3-trifluoro-2,2-dimethylpropionic acid (0.037 g, 0.24 mmol) and triethylamine (0.071 ml, 0.51 mmol) at room temperature, and the mixture was stirred for 1 hr. To the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=19/1âˆ’8/2) to give the title compound (0.077 g, yield 79%).

¹H-NMR (CDCl₃) Î´: 1.35 (6H, d, J=6.0 Hz), 1.44 (6H, br s), 3.37-3.49 (1H, m), 4.23 (3H, s), 4.56 (2H, d, J=5.8 Hz), 6.25 (1H, br s), 7.37 (1H, dd, J=8.4, 2.3 Hz), 7.54 (1H, d, J=8.4 Hz), 7.74 (1H, d, J=8.4 Hz), 7.96 (1H, d, J=2.3 Hz), 8.46 (1H, d, J=8.4 Hz), 8.72 (1H, br s).

(10) N-{4-chloro-3-[4-hydroxy-6-(3-isopropyl-4-trifluoromethylphenyl)-1,3,5-triazin-2-yl]benzyl}-3,3,3-trifluoro-2,2-dimethylpropionamide (Example No. 1-130)

[Image Omitted]

Under an argon atmosphere, to a solution of N-{4-chloro-3-[4-(3-isopropyl-4-trifluoromethylphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzyl}-3,3,3-trifluoro-2,2-dimethylpropionamide (0.077 g, 0.13 mmol) obtained in the above-mentioned (9) in methanol (0.80 ml) was added 4M aqueous sodium hydroxide solution (0.27 ml) at room temperature, and the mixture was stirred at 60Â° C. for 1 hr. To the reaction mixture were added 2N hydrochloric acid (0.54 ml) and water at room temperature, and the mixture was stirred. The precipitated solid was collected by filtration, washed with water, and dried under reduced pressure to give the title compound (0.066 g, yield 88%).

Production Example 17Synthesis of N-{3-[4-(4-butoxyphenyl)-6-hydroxy-1,3,5-triazin-2-yl]-4-chlorobenzyl}-3,3,3-trifluoro-2,2-dimethylpropionamide (Example No. 1-131)

[Image Omitted]

(1) N-{3-[4-(4-butoxyphenyl)-6-methoxy-1,3,5-triazin-2-yl]-4-chlorobenzyl}-3,3,3-trifluoro-2,2-dimethylpropionamide

[Image Omitted]

Under an argon atmosphere, to a solution of N-{4-chloro-3-[4-(4-hydroxyphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzyl}-3,3,3-trifluoro-2,2-dimethylpropionamide (0.10 g, 0.21 mmol) obtained in the above-mentioned [Production Example 14](6), n-butanol (0.023 ml, 0.25 mmol) and triphenylphosphine (0.066 g, 0.25 mmol) in tetrahydrofuran (1.0 ml) was added bis(2-methoxyethyl) azodicarboxylate (0.059 g, 0.25 mmol) under ice-cooling, and the mixture was stirred for 1 hr. To the reaction mixture were added n-butanol (0.019 ml, 0.21 mmol), triphenylphosphine (0.055 g, 0.21 mmol) and bis(2-methoxyethyl) azodicarboxylate (0.049 g, 0.21 mmol), and the mixture was stirred at room temperature for 2 hr. To the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated brine, dried over magnesium sulfate, filtered to remove magnesium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=1/1) to give the title compound (0.096 g, yield 85%).

(2) N-{3-[4-(4-butoxyphenyl)-6-hydroxy-1,3,5-triazin-2-yl]-4-chlorobenzyl}-3,3,3-trifluoro-2,2-dimethylpropionamide (Example No. 1-131)

[Image Omitted]

Under an argon atmosphere, to a solution of N-{3-[4-(4-butoxyphenyl)-6-methoxy-1,3,5-triazin-2-yl]-4-chlorobenzyl}-3,3,3-trifluoro-2,2-dimethylpropionamide (0.096 g, 0.18 mmol) obtained in the above-mentioned (1) in methanol (0.96 ml) was added 4M aqueous sodium hydroxide solution (0.27 ml) at room temperature, and the mixture was stirred at 65Â° C. for 2 hr. To the reaction mixture were added 2N hydrochloric acid (0.54 ml) and water and the mixture was stirred at room temperature. The precipitated solid was collected by filtration, washed with water, and dried under reduced pressure to give the title compound (0.086 g, yield 93%).

Production Example 18Synthesis of N-{4-chloro-3-[4-(3-cyclopropyl-4-fluorophenyl)-6-hydroxy-1,3,5-triazin-2-yl]benzyl}-3,3,3-trifluoro-2,2-dimethylpropionamide (Example No. 1-135)

[Image Omitted]

(1) 2-chloro-4-(3-cyclopropyl-4-fluorophenyl)-6-methoxy-1,3,5-triazine

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Under an argon atmosphere, to a suspension of 2-(3-cyclopropyl-4-fluorophenyl)-4,4,5,5-tetramethyl-[1,3,2]dioxaborolane (0.59 g, 2.2 mmol), 2,4-dichloro-6-methoxy-1,3,5-triazine (0.81 g, 4.5 mmol) and [1,1â€²-bis(diphenylphosphino) ferrocene]palladium(II)dichloride dichloromethane adduct (0.18 g, 0.22 mmol) in 1,4-dioxane (3.0 ml) was added 2M aqueous sodium carbonate solution (3.4 ml), and the mixture was stirred at 100Â° C. for 1 hr. At room temperature, to the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=25/1âˆ’20/1) to give the title compound as a crude product (0.44 g).

(2) {4-chloro-3-[4-(3-cyclopropyl-4-fluorophenyl)-6-methoxy-1,3,5-triazin-2-yl]phenyl}methanol

[Image Omitted]

Under an argon atmosphere, to a solution of a crude product (0.44 g) of 2-chloro-4-(3-cyclopropyl-4-fluorophenyl)-6-methoxy-1,3,5-triazine obtained in the above-mentioned (1), 2-chloro-5-hydroxymethylphenylboronic acid (0.31 g, 1.6 mmol) and [1,1â€²-bis (diphenylphosphino) ferrocene]palladium(II) dichloride dichloromethane adduct (0.11 g, 0.13 mmol) in 1,4-dioxane (5.4 ml) was added 2M aqueous sodium carbonate solution (2.7 ml), and the mixture was stirred at 100Â° C. for 1 hr. At room temperature, to the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=5/3) to give the title compound (0.32 g).

¹H-NMR (CDCl₃) Î´: 0.83-0.88 (2H, m), 1.01-1.07 (2H, m), 1.79 (1H, t, J=6.0 Hz), 2.10-2.19 (1H, m), 4.20 (3H, s), 4.77 (2H, d, J=6.0 Hz), 7.13 (1H, t, J=9.2 Hz), 7.47 (1H, d, J=8.1 Hz), 7.54 (1H, d, J=8.1 Hz), 8.01 (1H, br s), 8.20 (1H, d, J=7.6 Hz), 8.38-8.41 (1H, m).

(3) tert-butyl N-{4-chloro-3-[4-(3-cyclopropyl-4-fluorophenyl)-6-methoxy-1,3,5-triazin-2-yl]benzyl}-N-(tert-butoxycarbonyl)carbamate

[Image Omitted]

Under an argon atmosphere, to a solution of {4-chloro-3-[4-(3-cyclopropyl-4-fluorophenyl)-6-methoxy-1,3,5-triazin-2-yl]phenyl}methanol (0.32 g, 0.82 mmol) obtained in the above-mentioned (2) in tetrahydrofuran (3.3 ml) were added triethylamine (0.15 ml, 1.1 mmol) and methanesulfonyl chloride (0.076 ml, 0.98 mmol) under ice-cooling, and the mixture was stirred for 0.5 hr. The reaction mixture was filtered, and the filtrate was concentrated under reduced pressure. To a solution of the residue in N,N-dimethylformamide (3.3 ml) were added cesium carbonate (0.80 g, 2.5 mmol) and di-tert-butyl iminodicarboxylate (0.21 g, 0.98 mmol) at room temperature, and the mixture was stirred for 1 hr. To the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated brine, dried over magnesium sulfate, filtered to remove magnesium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=7/1) to give the title compound (0.40 g, yield 83%).

¹H-NMR (CDCl₃) Î´: 0.84-0.88 (2H, m), 1.01-1.07 (2H, m), 1.47 (18H, s), 2.09-2.18 (1H, m), 4.18 (3H, s), 4.83 (2H, s), 7.11 (1H, dd, J=9.7, 8.6 Hz), 7.40 (1H, dd, J=8.3, 2.2 Hz), 7.49 (1H, d, J=8.3 Hz), 8.00 (1H, d, J=2.2 Hz), 8.19 (1H, dd, J=7.5, 2.2 Hz), 8.36-8.41 (1H, m).

(4) 4-chloro-3-[4-(3-cyclopropyl-4-fluorophenyl)-6-methoxy-1,3,5-triazin-2-yl]benzylamine hydrochloride

[Image Omitted]

Under an argon atmosphere, to tert-butyl N-{4-chloro-3-[4-(3-cyclopropyl-4-fluorophenyl)-6-methoxy-1,3,5-triazin-2-yl]benzyl}-N-(tert-butoxycarbonyl)carbamate (0.40 g, 0.68 mmol) obtained in the above-mentioned (3) was added 4M hydrogen chloride/1,4-dioxane solution (3.3 ml) at room temperature, and the mixture was stirred for 1 hr. To the reaction mixture was added ethyl acetate (35 ml), and the mixture was stirred. The solid was collected by filtration and dried under reduced pressure to give the title compound (0.26 g, yield 89%).

¹H-NMR (DMSO-D₆) Î´: 0.78-0.83 (2H, m), 1.05-1.10 (2H, m), 2.10-2.19 (1H, m), 4.16 (3H, s), 4.16 (2H, s), 7.39 (1H, dd, J=9.9, 8.7 Hz), 7.71 (1H, dd, J=8.4, 2.1 Hz), 7.75 (1H, d, J=8.4 Hz), 8.13 (1H, dd, J=7.7, 2.1 Hz), 8.16 (1H, d, J=2.1 Hz), 8.35-8.37 (4H, m).

(5) N-{4-chloro-3-[4-(3-cyclopropyl-4-fluorophenyl)-6-methoxy-1,3,5-triazin-2-yl]benzyl}-3,3,3-trifluoro-2,2-dimethylpropionamide

[Image Omitted]

Under an argon atmosphere, to a solution of 4-chloro-3-[4-(3-cyclopropyl-4-fluorophenyl)-6-methoxy-1,3,5-triazin-2-yl]benzylamine hydrochloride (0.070 g, 0.17 mmol) obtained in the above-mentioned (4), HOBt.H₂O (0.033 g, 0.22 mmol) and WSC.HCl (0.041 g, 0.22 mmol) in N,N-dimethylformamide (2.0 ml) were added 3,3,3-trifluoro-2,2-dimethylpropionic acid (0.034 g, 0.22 mmol) and triethylamine (0.069 ml, 0.48 mmol) at room temperature, and the mixture was stirred for 3 hr. To the reaction mixture were added saturated aqueous sodium hydrogen carbonate and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=5/3) to give the title compound (0.082 g, yield 94%).

¹H-NMR (CDCl₃) Î´: 0.82-0.87 (2H, m), 1.01-1.05 (2H, m), 1.43 (6H, s), 2.10-2.16 (1H, m), 4.18 (3H, s), 4.54 (2H, d, J=5.6 Hz), 6.21 (1H, br s), 7.11 (1H, t, J=9.2 Hz), 7.34 (1H, d, J=8.3 Hz), 7.51 (1H, d, J=8.3 Hz), 7.90 (1H, s), 8.18 (1H, d, J=7.7 Hz), 8.36-8.40 (1H, m).

(6) N-{4-chloro-3-[4-(3-cyclopropyl-4-fluorophenyl)-6-hydroxy-1,3,5-triazin-2-yl]benzyl}-3,3,3-trifluoro-2,2-dimethylpropionamide (Example No. 1-135)

[Image Omitted]

Under an argon atmosphere, to a solution of N-{4-chloro-3-[4-(3-cyclopropyl-4-fluorophenyl)-6-methoxy-1,3,5-triazin-2-yl]benzyl}-3,3,3-trifluoro-2,2-dimethylpropionamide (0.082 g, 0.16 mmol) obtained in the above-mentioned (5) in methanol (1.8 ml) was added 4M aqueous sodium hydroxide solution (0.24 ml) at room temperature, and the mixture was stirred at 60Â° C. for 3 hr. To the reaction mixture were added 10% aqueous citric acid solution (1.0 ml) and water, and the mixture was stirred at room temperature. The precipitated solid was collected by filtration, washed with water, and dried under reduced pressure to give the title compound (0.065 g, yield 81%).

Production Example 19Synthesis of (R)â€”N-{4-chloro-3-[4-(4-chloro-3-methylphenyl)-6-hydroxy-1,3,5-triazin-2-yl]benzyl}-3,3,3-trifluoro-2-methoxy-2-methylpropionamide (Example No. 1-136)

[Image Omitted]

(1) benzyl (R)-3,3,3-trifluoro-2-hydroxy-2-methylpropionate

[Image Omitted]

Under an argon atmosphere, to a suspension of (R)-3,3,3-trifluoro-2-hydroxy-2-methylpropionic acid (2.2 g, 14 mmol) and potassium carbonate (2.3 g, 16 mmol) in N,N-dimethylformamide (30 ml) was added benzyl bromide (1.8 ml, 15 mmol) at room temperature, and the mixture was stirred for 4 hr. To the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=6/1) to give the title compound (3.0 g, yield 90%).

¹H-NMR (CDCl₃) Î´: 1.60 (3H, s), 3.78 (1H, s), 5.31 (2H, s), 7.33-7.42 (5H, m).

(2) benzyl (R)-3,3,3-trifluoro-2-methoxy-2-methylpropionate

[Image Omitted]

Under an argon atmosphere, to a solution of benzyl (R)-3,3,3-trifluoro-2-hydroxy-2-methylpropionate (3.4 g, 14 mmol) obtained in the above-mentioned (1) in N,N-dimethylformamide (40 ml) was added sodium hydride (0.60 g, 60 wt % oil dispersion) under ice-cooling, and the mixture was stirred for 1 hr. To the reaction mixture was added methyl iodide (1.3 ml, 20 mmol), and the mixture was stirred at room temperature for 2 hr. To the reaction mixture were added saturated aqueous ammonium chloride and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=15/1) to give the title compound (2.8 g, yield 78%).

¹H-NMR (CDCl₃) Î´: 1.59 (3H, s), 3.40 (3H, s), 5.26 (2H, s), 7.31-7.37 (5H, m).

(3) (R)-3,3,3-trifluoro-2-methoxy-2-methylpropionic acid

[Image Omitted]

Under an argon atmosphere, to a solution of benzyl (R)-3,3,3-trifluoro-2-methoxy-2-methylpropionate (2.8 g, 11 mmol) obtained in the above-mentioned (2) in ethyl acetate (50 ml) was added 10 wt % palladium carbon (0.23 g) at room temperature, and the mixture was stirred under 1 atm hydrogen atmosphere for 5 hr. Under a nitrogen atmosphere, the reaction mixture was filtered through celite and eluted with ethyl acetate. The filtrate was concentrated under reduced pressure to give the title compound (1.4 g, yield 78%).

¹H-NMR (CDCl₃) Î´: 1.68 (3H, s), 3.54 (3H, s).

(4) 2-chloro-4-(4-chloro-3-methylphenyl)-6-methoxy-1,3,5-triazine

[Image Omitted]

Under an argon atmosphere, to a suspension of 4-chloro-3-methylphenylboronic acid (0.47 g, 2.8 mmol), 2,4-dichloro-6-methoxy-1,3,5-triazine (1.0 g, 5.6 mmol) and tetrakis(triphenylphosphine)palladium(0) (0.32 g, 0.28 mmol) in toluene (5.0 ml) was added 2M aqueous sodium carbonate solution (4.2 ml), and the mixture was stirred at 100Â° C. for 2 hr. At room temperature, to the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=98/2âˆ’95/5) to give the title compound (0.50 g, yield 48%). ¹H-NMR (CDCl₃) Î´: 2.47 (3H, s), 4.17 (3H, s), 7.47 (1H, d, J=8.4 Hz), 8.26 (1H, dd, J=8.4, 2.1 Hz), 8.36 (1H, d, J=2.1 Hz).

(5) {4-chloro-3-[4-(4-chloro-3-methylphenyl)-6-methoxy-1,3,5-triazin-2-yl]phenyl}methanol

[Image Omitted]

Under an argon atmosphere, to a solution of 2-chloro-4-(4-chloro-3-methylphenyl)-6-methoxy-1,3,5-triazine (0.50 g, 1.3 mmol) obtained in the above-mentioned (4), 2-chloro-5-hydroxymethylphenylboronic acid (0.30 g, 1.6 mmol) and [1,1â€²-bis(diphenylphosphino) ferrocene]palladium(II)dichloride dichloromethane adduct (0.11 g, 0.13 mmol) in 1,4-dioxane (5.0 ml) was added 2M aqueous sodium carbonate solution (2.6 ml), and the mixture was stirred at 100Â° C. for 1.5 hr. At room temperature, to the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=8/2âˆ’6/4) to give the title compound (0.40 g, yield 80%).

¹H-NMR (CDCl₃) Î´: 1.79 (1H, t, J=5.3 Hz), 2.48 (3H, s), 4.21 (3H, s), 4.78 (2H, d, J=5.3 Hz), 7.45-7.50 (2H, m), 7.54 (1H, d, J=8.1 Hz), 8.01 (1H, d, J=2.1 Hz), 8.37 (1H, dd, J=8.4, 2.1 Hz), 8.46 (1H, d, J=2.1 Hz).

(6) 4-chloro-3-[4-(4-chloro-3-methylphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzylamine hydrochloride

[Image Omitted]

Under an argon atmosphere, to a solution of {4-chloro-3-[4-(4-chloro-3-methylphenyl)-6-methoxy-1,3,5-triazin-2-yl]phenyl}methanol (0.40 g, 1.1 mmol) obtained in the above-mentioned (5) in tetrahydrofuran (4.0 ml) were added triethylamine (0.19 ml, 1.4 mmol) and methanesulfonyl chloride (0.098 ml, 1.3 mmol) under ice-cooling, and the mixture was stirred for 1 hr. The reaction mixture was filtered, and the filtrate was concentrated under reduced pressure. To a solution of the residue in N,N-dimethylformamide (2.0 ml) were added cesium carbonate (1.0 g, 3.2 mmol) and di-tert-butyl iminodicarboxylate (0.37 g, 1.7 mmol) at room temperature, and the mixture was stirred for 3 hr. To the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=95/5âˆ’80/20). Under an argon atmosphere, to a solution (2.0 ml) of the purified product in 1,4-dioxane was added 4M hydrogen chloride/1,4-dioxane solution (4.0 ml) at room temperature, and the mixture was stirred for 2 hr. To the reaction mixture was added n-hexane, and the solid was collected by filtration and dried under reduced pressure to give the title compound (0.43 g, yield 99%).

¹H-NMR (DMSO-D₆) Î´: 2.47 (3H, s), 4.13-4.19 (5H, m), 7.67 (1H, d, J=8.3 Hz), 7.71-7.76 (2H, m), 8.16 (1H, d, J=1.6 Hz), 8.35 (1H, dd, J=8.3, 1.6 Hz), 8.41-8.50 (4H, m).

(7) (R)â€”N-{4-chloro-3-[4-(4-chloro-3-methylphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzyl}-3,3,3-trifluoro-2-methoxy-2-methylpropionamide

[Image Omitted]

Under an argon atmosphere, to a solution of 4-chloro-3-[4-(4-chloro-3-methylphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzylamine hydrochloride (0.070 g, 0.17 mmol) obtained in the above-mentioned (6), HOBt.H₂O (0.039 g, 0.26 mmol) and WSC.HCl (0.049 g, 0.26 mmol) in N,N-dimethylformamide (0.70 ml) were added (R)-3,3,3-trifluoro-2-methoxy-2-methylpropionic acid (0.038 g, 0.22 mmol) obtained in the above-mentioned (3) and triethylamine (0.071 ml, 0.51 mmol) at room temperature, and the mixture was stirred for 18 hr. To the reaction mixture were added saturated aqueous sodium hydrogen carbonate and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=3/2) to give the title compound (0.058 g, yield 65%).

¹H-NMR (CDCl₃) Î´: 1.66 (3H, s), 2.48 (3H, s), 3.45 (3H, s), 4.20 (3H, s), 4.48 (1H, dd, J=15.1, 5.8 Hz), 4.63 (1H, dd, J=15.1, 6.5 Hz), 7.16 (1H, br s), 7.37 (1H, dd, J=8.3, 2.3 Hz), 7.48 (1H, d, J=8.3 Hz), 7.53 (1H, d, J=8.3 Hz), 7.92 (1H, d, J=2.3 Hz), 8.36 (1H, dd, J=8.3, 2.0 Hz), 8.46 (1H, d, J=2.0 Hz).

(8) (R)â€”N-{4-chloro-3-[4-(4-chloro-3-methylphenyl)-6-hydroxy-1,3,5-triazin-2-yl]benzyl}-3,3,3-trifluoro-2-methoxy-2-methylpropionamide (Example No. 1-136)

[Image Omitted]

Under an argon atmosphere, to a solution of (R)â€”N-{4-chloro-3-[4-(4-chloro-3-methylphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzyl}-3,3,3-trifluoro-2-methoxy-2-methylpropionamide (0.058 g, 0.11 mmol) obtained in the above-mentioned (7) in methanol (1.3 ml) was added 4M aqueous sodium hydroxide solution (0.17 ml) at room temperature, and the mixture was stirred at 60Â° C. for 3 hr. To the reaction mixture were added 10% aqueous citric acid solution (0.68 ml) and water, and the mixture was stirred at room temperature. The precipitated solid was collected by filtration, washed with water, and dried under reduced pressure to give the title compound (0.051 g, yield 88%).

Production Example 20Synthesis of (R)â€”N-{4-chloro-3-[4-hydroxy-6-(4-propoxyphenyl)-1,3,5-triazin-2-yl]benzyl}-3,3,3-trifluoro-2-methoxy-2-methylpropionamide (Example No. 1-137)

[Image Omitted]

(1) (R)â€”N-{4-chloro-3-[4-methoxy-6-(4-propoxyphenyl)-1,3,5-triazin-2-yl]benzyl}-3,3,3-trifluoro-2-methoxy-2-methylpropionamide

[Image Omitted]

Under an argon atmosphere, to a solution of 4-chloro-3-[4-methoxy-6-(4-propoxyphenyl)-1,3,5-triazin-2-yl]benzylamine hydrochloride (0.80 g, 0.19 mmol) obtained in [Production Example 13](4), HOBt.H₂O (0.044 g, 0.28 mmol) and WSC.HCl (0.055 g, 0.28 mmol) in N,N-dimethylformamide (1.0 ml) were added (R)-3,3,3-trifluoro-2-methoxy-2-methylpropionic acid (0.046 g, 0.27 mmol) obtained in [Production Example 19](3) and triethylamine (0.080 ml, 0.57 mmol) at room temperature, and the mixture was stirred for 18 hr. To the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=8/2âˆ’3/2) to give the title compound (0.084 g, yield 82%).

¹H-NMR (CDCl₃) Î´: 1.07 (3H, t, J=7.4 Hz), 1.66 (3H, br s), 1.81-1.90 (2H, m), 3.45 (3H, br s), 4.02 (2H, t, J=6.5 Hz), 4.19 (3H, s), 4.50 (1H, dd, J=15.0, 5.8 Hz), 4.59 (1H, dd, J=15.0, 6.3 Hz), 6.97-7.02 (2H, m), 7.14 (1H, br s), 7.35 (1H, dd, J=8.3, 2.3 Hz), 7.51 (1H, d, J=8.3 Hz), 7.92 (1H, d, J=2.3 Hz), 8.52-8.56 (2H, m).

(2) (R)â€”N-{4-chloro-3-[4-hydroxy-6-(4-propoxyphenyl)-1,3,5-triazin-2-yl]benzyl}-3,3,3-trifluoro-2-methoxy-2-methylpropionamide (Example No. 1-137)

[Image Omitted]

Under an argon atmosphere, to a solution of (R)â€”N-{4-chloro-3-[4-methoxy-6-(4-propoxyphenyl)-1,3,5-triazin-2-yl]benzyl}-3,3,3-trifluoro-2-methoxy-2-methylpropionamide (0.084 g, 0.16 mmol) obtained in the above-mentioned (1) in methanol (0.80 ml) was added 4M aqueous sodium hydroxide solution (0.30 ml) at room temperature, and the mixture was stirred at 65Â° C. for 1.5 hr. To the reaction mixture were added 2N hydrochloric acid (0.60 ml) and water, and the mixture was stirred at room temperature. The precipitated solid was collected by filtration, washed with water, and dried under reduced pressure to give the title compound (0.072 g, yield 89%).

Production Example 21Synthesis of N-{4-chloro-3-[4-(3,4-dimethylphenyl)-6-hydroxy-1,3,5-triazin-2-yl]benzyl}-3,3,3-trifluoro-2,2-dimethylpropionamide (Example No. 1-150)

[Image Omitted]

(1) 2-chloro-4-(3,4-dimethylphenyl)-6-methoxy-1,3,5-triazine

[Image Omitted]

Under an argon atmosphere, to a suspension of 3,4-dimethylbenzeneboronic acid (0.42 g, 2.8 mmol), 2,4-dichloro-6-methoxy-1,3,5-triazine (1.0 g, 5.6 mmol) and tetrakis(triphenylphosphine)palladium(0) (0.32 g, 0.28 mmol) in toluene (8.4 ml) was added 2M aqueous sodium carbonate solution (4.2 ml), and the mixture was stirred at 100Â° C. for 2 hr. At room temperature, to the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=20/1) to give the title compound (0.64 g, 92%).

¹H-NMR (CDCl₃) Î´: 2.35 (6H, s), 4.16 (3H, s), 7.26 (3H, d, J=7.8 Hz), 8.22 (1H, dd, J=7.8, 2.1 Hz), 8.25 (1H, d, J=2.1 Hz).

(2) {4-chloro-3-[4-(3,4-dimethylphenyl)-6-methoxy-1,3,5-triazin-2-yl]phenyl}methanol

[Image Omitted]

Under an argon atmosphere, to a solution of 2-chloro-4-(3,4-dimethylphenyl)-6-methoxy-1,3,5-triazine (0.64 g, 2.6 mmol) obtained in the above-mentioned (1), 2-chloro-5-hydroxymethylphenylboronic acid (0.57 g, 3.1 mmol) and [1,1â€²-bis (diphenylphosphino) ferrocene]palladium(II)dichloride dichloromethane adduct (0.21 g, 0.26 mmol) in 1,4-dioxane (10 ml) was added 2M aqueous sodium carbonate solution (5.1 ml), and the mixture was stirred at 100Â° C. for 1 hr. At room temperature, to the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=5/3) to give the title compound (0.54 g, yield 59%).

¹H-NMR (CDCl₃) Î´: 1.87 (1H, t, J=5.0 Hz), 2.35 (3H, s), 2.36 (3H, s), 4.20 (3H, s), 4.76 (2H, d, J=5.0 Hz), 7.27 (2H, d, J=8.2 Hz), 7.45 (1H, dd, J=8.4, 1.6 Hz), 7.53 (1H, d, J=8.4 Hz), 7.99 (1H, d, J=1.6 Hz), 8.33 (1H, d, J=8.2 Hz), 8.35 (1H, br s).

(3) 4-chloro-3-[4-(3,4-dimethylphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzylamine hydrochloride

[Image Omitted]

Under an argon atmosphere, to a solution of {4-chloro-3-[4-(3,4-dimethylphenyl)-6-methoxy-1,3,5-triazin-2-yl]phenyl}methanol (0.54 g, 1.5 mmol) obtained in the above-mentioned (2) in tetrahydrofuran (5.5 ml) were added triethylamine (0.28 ml, 2.0 mmol) and methanesulfonyl chloride (0.14 ml, 1.8 mmol) under ice-cooling, and the mixture was stirred for 0.5 hr. The reaction mixture was filtered, and the filtrate was concentrated under reduced pressure. To a solution of the residue in N,N-dimethylformamide (5.5 ml) were added cesium carbonate (1.5 g, 4.6 mmol) and di-tert-butyl iminodicarboxylate (0.40 g, 1.8 mmol) under ice-cooling, and the mixture was stirred at room temperature for 1 hr. To the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=7/1). Under an argon atmosphere, to the purified product was added 4M hydrogen chloride/1,4-dioxane solution (6.5 ml) at room temperature, and the mixture was stirred for 0.5 hr. To the reaction mixture was added ethyl acetate, and the solid was collected by filtration, and dried under reduced pressure to give the title compound (0.56 g, yield 94%).

¹H-NMR (DMSO-D6) Î´: 2.34 (3H, s), 2.35 (3H, s), 4.12-4.19 (5H, m), 7.38 (1H, d, J=7.9 Hz), 7.69-7.75 (2H, m), 8.12 (1H, d, J=1.9 Hz), 8.26 (1H, dd, J=7.9, 1.6 Hz), 8.29 (1H, br s), 8.44 (3H, br s).

(4) N-{4-chloro-3-[4-(3,4-dimethylphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzyl}-3,3,3-trifluoro-2,2-dimethylpropionamide

[Image Omitted]

Under an argon atmosphere, to a solution of 4-chloro-3-[4-(3,4-dimethylphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzylamine hydrochloride (0.070 g, 0.18 mmol) obtained in the above-mentioned (3), HOBt.H₂O (0.035 g, 0.23 mmol) and WSC.HCl (0.044 g, 0.23 mmol) in N,N-dimethylformamide (2.0 ml) were added 3,3,3-trifluoro-2,2-dimethylpropionic acid (0.036 g, 0.23 mmol) and triethylamine (0.075 ml, 0.54 mmol) at room temperature, and the mixture was stirred for 4 hr. To the reaction mixture were added saturated aqueous sodium hydrogen carbonate and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=2/1) to give the title compound (0.075 g, yield 85%).

¹H-NMR (CDCl₃) Î´: 1.44 (6H, s), 2.36 (3H, s), 2.37 (3H, s), 4.20 (3H, s), 4.55 (2H, d, J=5.7 Hz), 6.22 (1H, br s), 7.27 (3H, d, J=7.8 Hz), 7.35 (1H, dd, J=8.2, 2.2 Hz), 7.52 (1H, d, J=8.2 Hz), 7.91 (1H, d, J=2.2 Hz), 8.32 (1H, dd, J=7.8, 1.7 Hz), 8.35 (1H, br s).

(5) N-{4-chloro-3-[4-(3,4-dimethylphenyl)-6-hydroxy-1,3,5-triazin-2-yl]benzyl}-3,3,3-trifluoro-2,2-dimethylpropionamide (Example No. 1-150)

[Image Omitted]

Under an argon atmosphere, to a solution of N-{4-chloro-3-[4-(3,4-dimethylphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzyl}-3,3,3-trifluoro-2,2-dimethylpropionamide (0.075 g, 0.15 mmol) obtained in the above-mentioned (4) in methanol (1.8 ml) was added 4M aqueous sodium hydroxide solution (0.23 ml) at room temperature, and the mixture was stirred at 60Â° C. for 4 hr. To the reaction mixture were added 10% aqueous citric acid solution (1.0 ml) and water at room temperature, and the mixture was stirred. The precipitated solid was collected by filtration, washed with water, and dried under reduced pressure to give the title compound (0.063 g, yield 86%).

Production Example 22Synthesis of N-{4-chloro-3-[4-(4-cyclopropylmethoxyphenyl)-6-hydroxy-1,3,5-triazin-2-yl]benzyl}-3,3,3-trifluoro-2-methyl-2-trifluoromethylpropionamide (Example No. 1-169)

[Image Omitted]

(1) 2-chloro-4-(4-cyclopropylmethoxyphenyl)-6-methoxy-1,3,5-triazine

[Image Omitted]

Under an argon atmosphere, to a suspension of 4-(cyclopropylmethoxy)benzeneboronic acid (2.5 g, 13 mmol), 2,4-dichloro-6-methoxy-1,3,5-triazine (4.7 g, 26 mmol) and tetrakis(triphenylphosphine)palladium(0) (1.5 g, 1.3 mmol) in toluene (25 ml) was added 2M aqueous sodium carbonate solution (20 ml), and the mixture was stirred at 100Â° C. for 2 hr. At room temperature, to the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=90/10âˆ’80/20) to give the title compound (3.0 g, 79%).

¹H-NMR (CDCl₃) Î´: 0.36-0.41 (2H, m), 0.65-0.71 (2H, m), 1.25-1.36 (1H, m), 3.90 (2H, d, J=7.0 Hz), 4.14 (3H, s), 6.96-7.00 (2H, m), 8.42-8.47 (2H, m).

(2) {4-chloro-3-[4-(4-cyclopropylmethoxyphenyl)-6-methoxy-1,3,5-triazin-2-yl]phenyl}methanol

[Image Omitted]

Under an argon atmosphere, to a solution of 2-chloro-4-(4-cyclopropylmethoxyphenyl)-6-methoxy-1,3,5-triazine (3.0 g, 10 mmol) obtained in the above-mentioned (1), 2-chloro-5-hydroxymethylphenylboronic acid (2.3 g, 12 mmol) and [1,1â€²-bis(diphenylphosphino)ferrocene]palladium(II)dichloride dichloromethane adduct (0.84 g, 1.0 mmol) in 1,4-dioxane (30 ml) was added 2M aqueous sodium carbonate solution (21 ml), and the mixture was stirred at 100Â° C. for 3 hr. At room temperature, to the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=8/2âˆ’1/1) to give the title compound (2.9 g, yield 71%).

¹H-NMR (CDCl₃) Î´: 0.37-0.41 (2H, m), 0.65-0.71 (2H, m), 1.27-1.36 (1H, m), 1.76 (1H, t, J=6.0 Hz), 3.90 (2H, d, J=6.7 Hz), 4.19 (3H, s), 4.77 (2H, d, J=6.0 Hz), 6.98-7.02 (2H, m), 7.46 (1H, dd, J=8.1, 1.9 Hz), 7.53 (1H, d, J=8.1 Hz), 8.00 (1H, d, J=1.9 Hz), 8.53-8.57 (2H, m).

(3) 4-chloro-3-[4-(4-cyclopropylmethoxyphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzylamine hydrochloride

[Image Omitted]

Under an argon atmosphere, to a solution of {4-chloro-3-[4-(4-cyclopropylmethoxyphenyl)-6-methoxy-1,3,5-triazin-2-yl]phenyl}methanol (2.9 g, 7.3 mmol) obtained in the above-mentioned (2) in tetrahydrofuran (29 ml) were added triethylamine (1.3 ml, 9.5 mmol) and methanesulfonyl chloride (0.68 ml, 8.7 mmol) under ice-cooling, and the mixture was stirred for 0.5 hr. The reaction mixture was filtered, and the filtrate was concentrated under reduced pressure. To a solution of the residue in N,N-dimethylformamide (29 ml) were added cesium carbonate (7.1 g, 22 mmol) and di-tert-butyl iminodicarboxylate (1.9 g, 8.7 mmol) under ice-cooling, and the mixture was stirred at room temperature for 2 hr. To the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=95/5âˆ’70/30). Under an argon atmosphere, to a solution (9.3 ml) of the purified product in 1,4-dioxane was added 4M hydrogen chloride/1,4-dioxane solution (37 ml) at room temperature, and the mixture was stirred for 3 hr. To the reaction mixture was added ethyl acetate, and the solid was collected by filtration, and dried under reduced pressure to give the title compound (3.1 g, yield 97%).

¹H-NMR (DMSO-D₆) Î´: 0.34-0.39 (2H, m), 0.57-0.63 (2H, m), 1.21-1.32 (1H, m), 3.95 (2H, d, J=7.0 Hz), 4.11-4.18 (5H, m), 7.11-7.15 (2H, m), 7.70-7.74 (2H, m), 8.13 (1H, br s), 8.42-8.53 (5H, m).

(4) N-{4-chloro-3-[4-(4-cyclopropylmethoxyphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzyl}-3,3,3-trifluoro-2-methyl-2-tri fluoromethylpropionamide

[Image Omitted]

Under an argon atmosphere, to a solution of 4-chloro-3-[4-(4-cyclopropylmethoxyphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzylamine hydrochloride (0.080 g, 0.18 mmol) obtained in the above-mentioned (3), HOBt.H₂O (0.037 g, 0.24 mmol) and WSC.HCl (0.046 g, 0.24 mmol) in N,N-dimethylformamide (2.0 ml) were added 2,2-bis(trifluoromethyl)propionic acid (0.050 g, 0.24 mmol) and triethylamine (0.077 ml, 0.55 mmol) at room temperature, and the mixture was stirred for 1.5 hr. To the reaction mixture were added HOBt.H₂O (0.037 g, 0.24 mmol), WSC.HCl (0.046 g, 0.24 mmol), 2,2-bis(trifluoromethyl)propionic acid (0.050 g, 0.24 mmol) and triethylamine (0.077 ml, 0.55 mmol), and the mixture was stirred for 2 hr. To the reaction mixture were added HOBt.H₂O (0.037 g, 0.24 mmol), WSC.HCl (0.046 g, 0.24 mmol), 2,2-bis(trifluoromethyl)propionic acid (0.050 g, 0.24 mmol) and triethylamine (0.077 ml, 0.55 mmol), and the mixture was stirred for 1.5 hr. To the reaction mixture were added saturated aqueous sodium hydrogen carbonate and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=3/1) to give the title compound (0.045 g, yield 41%).

¹H-NMR (CDCl₃) Î´: 0.36-0.41 (2H, m), 0.65-0.71 (2H, m), 1.26-1.35 (2H, m), 1.70 (3H, s), 3.90 (2H, d, J=6.7 Hz), 4.19 (3H, s), 4.61 (2H, d, J=5.8 Hz), 6.49 (1H, br s), 6.98-7.02 (2H, m), 7.32 (1H, dd, J=8.5, 2.1 Hz), 7.53 (1H, d, J=8.5 Hz), 7.92 (1H, d, J=2.1 Hz), 8.52-8.56 (2H, m).

(5) N-{4-chloro-3-[4-(4-cyclopropylmethoxyphenyl)-6-hydroxy-1,3,5-triazin-2-yl]benzyl}-3,3,3-trifluoro-2-methyl-2-trifluoromethylpropionamide (Example No. 1-169)

[Image Omitted]

Under an argon atmosphere, to a solution of N-{4-chloro-3-[4-(4-cyclopropylmethoxyphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzyl}-3,3,3-trifluoro-2-methyl-2-trifluoromethylpropionamide (0.045 g, 0.076 mmol) obtained in the above-mentioned (4) in methanol (0.70 ml) was added 4M aqueous sodium hydroxide solution (0.11 ml) at room temperature, and the mixture was stirred at 60Â° C. for 4 hr. To the reaction mixture were added 10% aqueous citric acid solution (0.50 ml) and water, and the mixture was stirred at room temperature. The precipitated solid was collected by filtration, washed with water, and dried under reduced pressure to give the title compound (0.039 g, yield 89%).

Production Example 23Synthesis of 1-trifluoromethylcyclopropanecarboxylic acid 4-chloro-3-[4-hydroxy-6-(4-isobutoxyphenyl)-1,3,5-triazin-2-yl]benzylamide (Example No. 1-178)

[Image Omitted]

(1) 1-trifluoromethylcyclopropanecarboxylic acid 4-chloro-3-[4-(4-isobutoxyphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzylamide

[Image Omitted]

Under an argon atmosphere, to a solution of 4-chloro-3-[4-(4-isobutoxyphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzylamine hydrochloride (0.10 g, 0.23 mmol) obtained in [Production Example 12](4), HOBt.H₂O (0.049 g, 0.32 mmol) and WSC.HCl (0.061 g, 0.32 mmol) in N,N-dimethylformamide (0.75 ml) were added 1-trifluoromethylcyclopropane-1-carboxylic acid (0.050 g, 0.32 mmol) and triethylamine (0.064 ml, 0.46 mmol) at room temperature, and the mixture was stirred for 1.5 hr. To the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=2/1, then chloroform/ethyl acetate=9/1) to give the title compound (0.068 g, yield 55%). ¹H-NMR (DMSO-D₆) Î´: 1.01 (6H, d, J=6.9 Hz), 1.23-1.27 (2H, m), 1.30-1.36 (2H, m), 2.00-2.11 (1H, m), 3.87 (2H, d, J=6.4 Hz), 4.12 (3H, s), 4.37 (2H, d, J=5.9 Hz), 7.11-7.15 (2H, m), 7.43 (1H, dd, J=8.2, 2.1 Hz), 7.60 (1H, d, J=8.2 Hz), 7.85 (1H, d, J=2.1 Hz), 8.43-8.47 (2H, m), 8.50 (1H, t, J=5.9 Hz).

(2) 1-trifluoromethyl-cyclopropanecarboxylic acid 4-chloro-3-[4-hydroxy-6-(4-isobutoxyphenyl)-1,3,5-triazin-2-yl]benzylamide (Example No. 1-178)

[Image Omitted]

Under an argon atmosphere, to a solution of 1-trifluoromethylcyclopropanecarboxylic acid 4-chloro-3-[4-(4-isobutoxyphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzylamide (0.065 g, 0.12 mmol) obtained in the above-mentioned (1) in methanol (1.0 ml) was added 4M aqueous sodium hydroxide solution (0.12 ml) at room temperature, and the mixture was stirred at 60Â° C. for 3 hr. To the reaction mixture were added 2N hydrochloric acid (0.24 ml) and water at room temperature, and the mixture was stirred. The precipitated solid was collected by filtration, washed with water, and dried under reduced pressure to give the title compound (0.060 g, yield 94%).

Production Example 24Synthesis of N-(4-chloro-3-{4-[4-((S)-1-cyclopropylethoxy)phenyl]-6-hydroxy-1,3,5-triazin-2-yl}benzyl)-3,3,3-trifluoro-2,2-dimethylpropionamide (Example No. 1-184)

[Image Omitted]

(1) N-(4-chloro-3-{4-[4-((S)-1-cyclopropylethoxy)phenyl]-6-methoxy-1,3,5-triazin-2-yl}benzyl)-3,3,3-trifluoro-2,2-dimethylpropionamide

[Image Omitted]

Under an argon atmosphere, to a solution of N-{4-chloro-3-[4-(4-hydroxyphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzyl}-3,3,3-trifluoro-2,2-dimethylpropionamide (0.080 g, 0.17 mmol) obtained in the above-mentioned [Production Example 14](6), (1R)-1-cyclopropylethan-1-ol (0.029 g, 0.33 mmol) and triphenylphosphine (0.087 g, 0.33 mmol) in tetrahydrofuran (1.0 ml) was added bis(2-methoxyethyl)azodicarboxylate (0.078 g, 0.33 mmol) under ice-cooling, and the mixture was stirred at room temperature for 17 hr. To the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=2/1) to give the title compound (0.079 g, yield 86%).

¹H-NMR (CDCl₃) Î´: 0.28-0.36 (1H, m), 0.38-0.45 (1H, m), 0.53-0.63 (2H, m), 1.12-1.21 (1H, m), 1.41 (3H, d, J=6.0 Hz), 1.44 (6H, s), 3.95-4.05 (1H, m), 4.18 (3H, s), 4.54 (2H, d, J=5.6 Hz), 6.20 (1H, br s), 6.95-7.00 (2H, m), 7.34 (1H, dd, J=8.3, 1.9 Hz), 7.51 (1H, d, J=8.3 Hz), 7.91 (1H, d, J=1.9 Hz), 8.50-8.55 (2H, m).

(2) N-(4-chloro-3-{4-[4-((S)-1-cyclopropylethoxy)phenyl]-6-hydroxy-1,3,5-triazin-2-yl}benzyl)-3,3,3-trifluoro-2,2-dimethylpropionamide (Example No. 1-184)

[Image Omitted]

Under an argon atmosphere, to a solution of N-(4-chloro-3-{4-[4-((S)-1-cyclopropylethoxy)phenyl]-6-methoxy-1,3,5-triazin-2-yl}benzyl)-3,3,3-trifluoro-2,2-dimethylpropionamide (0.079 g, 0.14 mmol) obtained in the above-mentioned (1) in methanol (1.3 ml) was added 4M aqueous sodium hydroxide solution (0.22 ml) at room temperature, and the mixture was stirred at 65Â° C. for 4 hr. To the reaction mixture were added 10% aqueous citric acid solution (0.90 ml) and water at room temperature, and the mixture was stirred. The precipitated solid was collected by filtration, washed with water, and dried under reduced pressure to give the title compound (0.072 g, yield 93%).

Production Example 25Synthesis of N-(4-chloro-3-{4-[4-((R)-1-cyclopropylethoxy)phenyl]-6-hydroxy-1,3,5-triazin-2-yl}benzyl)-3,3,3-trifluoro-2,2-dimethylpropionamide (Example No. 1-185)

[Image Omitted]

(1) N-(4-chloro-3-{4-[4-((R)-1-cyclopropylethoxy)phenyl]-6-methoxy-1,3,5-triazin-2-yl}benzyl)-3,3,3-trifluoro-2,2-dimethylpropionamide

[Image Omitted]

Under an argon atmosphere, to a solution of N-{4-chloro-3-[4-(4-hydroxyphenyl)-6-methoxy-1,3,5-triazin-2-yl]benzyl}-3,3,3-trifluoro-2,2-dimethylpropionamide (0.080 g, 0.17 mmol) obtained in the above-mentioned [Production Example 14](6), (1S)-1-cyclopropylethan-1-ol (0.029 g, 0.33 mmol) and triphenylphosphine (0.087 g, 0.33 mmol) in tetrahydrofuran (1.0 ml) was added bis(2-methoxyethyl) azodicarboxylate (0.078 g, 0.33 mmol) under ice-cooling, and the mixture was stirred at room temperature for 17 hr. To the reaction mixture were added water and ethyl acetate, and the mixture was partitioned. The organic layer was washed with saturated brine, dried over sodium sulfate, filtered to remove sodium sulfate, and concentrated under reduced pressure. The residue was purified by silica gel column chromatography (eluent: n-hexane/ethyl acetate=2/1) to give the title compound (0.038 g, yield 41%).

¹H-NMR (CDCl₃) Î´: 0.28-0.36 (1H, m), 0.38-0.45 (1H, m), 0.53-0.63 (2H, m), 1.12-1.21 (1H, m), 1.41 (3H, d, J=6.0 Hz), 1.44 (6H, s), 3.95-4.05 (1H, m), 4.18 (3H, s), 4.54 (2H, d, J=5.6 Hz), 6.20 (1H, br s), 6.95-7.00 (2H, m), 7.34 (1H, dd, J=8.3, 1.9 Hz), 7.51 (1H, d, J=8.3 Hz), 7.91 (1H, d, J=1.9 Hz), 8.50-8.55 (2H, m).

(2) N-(4-chloro-3-{4-[4-((R)-1-cyclopropylethoxy)phenyl]-6-hydroxy-1,3,5-triazin-2-yl}benzyl)-3,3,3-trifluoro-2,2-dimethylpropionamide (Example No. 1-185)

[Image Omitted]

Under an argon atmosphere, to a solution of N-(4-chloro-3-{4-[4-((R)-1-cyclopropylethoxy)phenyl]-6-methoxy-1,3,5-triazin-2-yl}benzyl)-3,3,3-trifluoro-2,2-dimethylpropionamide (0.038 g, 0.069 mmol) obtained in the above-mentioned (1) in methanol (0.62 ml) was added 4M aqueous sodium hydroxide solution (0.10 ml) at room temperature, and the mixture was stirred at 65Â° C. for 4 hr. To the reaction mixture were added 10% aqueous citric acid solution (0.42 ml) and water at room temperature, and the mixture was stirred. The precipitated solid was collected by filtration, washed with water, and dried under reduced pressure to give the title compound (0.034 g, yield 91%).

The compounds of Example 1-1 to Example 1-267, Example 2-1 to Example 2-130, and Example 3-1 to Example 3-23 were obtained according to the above-mentioned Production Methods. The structural formulas and property data of the Example compounds are shown in Table 1-1 to Table 1-34, Table 2-1 to Table 2-15, and Table 3-1 to Table 3-3. In the Tables, the notes show the following.

[Image Omitted]

Note 1 (Example No. 1-188, 1-189)

Using 2,4-dichloro-6-methoxy-1,3,5-triazine, 4-fluoro-3-methylphenylboronic acid instead of 4-(2,2-dimethylpropoxyl)phenylboronic acid, and 5-acetyl-2-chlorophenylboronic acid instead of 2-chloro-5-hydroxymethylphenylboronic acid, and by a method similar to that in Production Example 1 (1) and (2), compound A was obtained.

Racemic compound B was obtained by reducing the carbonyl group of compound A with sodium borohydride.

Racemic compound D was obtained by treating compound B in the same manner as in Production Example 1 (3) and (4).

Compound F as a diastereomer mixture was obtained by reacting racemic compound D with pure enantiomer compound E.

Compound F1 which is a less polar diastereomer (Merck TLC Silica gel 60G F254 25 Glassplates, eluent: n-hexane/ethyl acetate=2/1) and compound F2 which is a more polar diastereomer were obtained by purifying compound F by silica gel column chromatography. While compound F1 and compound F2 are single stereoisomers, the absolute configuration of the asymmetric carbon at the benzyl position is undetermined.

The compound of Example No. 1-188 was obtained by hydrolyzing compound F1 in the same manner as in Production Example 1 (6). Similarly, the compound of Example No. 1-189 was obtained from compound F2. While the compound of Example No. 1-188 and the compound of Example No. 1-189 are single stereoisomers, the absolute configuration of the asymmetric carbon at the benzyl position is undetermined.

[Image Omitted]

Note 2 (Example No. 1-200, 1-201)

Using 2,4-dichloro-6-methoxy-1,3,5-triazine, 4-(2,2-dimethylpropoxyl)phenylboronic acid, and 5-acetyl-2-chlorophenylboronic acid instead of 2-chloro-5-hydroxymethylphenylboronic acid, and by a method similar to that in Production Example 1 (1) and (2), compound J was obtained.

Racemic compound K was obtained by reducing the carbonyl group of compound J with sodium borohydride.

Racemic compound M was obtained by treating compound K in the same manner as in Production Example 1 (3) and (4).

Compound N as a diastereomer mixture was obtained by reacting racemic compound M with pure enantiomer compound E.

Compound N was purified by silica gel column chromatography in the same manner as in note 1, by a method similar to that in Production Example 1 (6), the compound of Example No. 1-200 was obtained from compound N1 which is a less polar diastereomer (Merck TLC Silica gel 60G F254 25 Glassplates, eluent: n-hexane/ethyl acetate=2/1), and the compound of Example No. 1-201 was obtained from compound N2 which is a more polar diastereomer. While the compounds of Example Nos. 1-200 and 1-201 are single stereoisomers, the absolute configuration of the asymmetric carbon at the benzyl position is undetermined.

Note 3 (Example Nos. 1-256, 1-257)

While they are single stereoisomers, the relative configuration thereof is undetermined.

Note 4 (Example No. 1-266)

While it is a single stereoisomer, the relative configuration of the tert-butyl group is undetermined.

Note 5 (Example No. 1-267)

While it is a single stereoisomer, the relative configuration of the methoxy group is undetermined.

TABLE 1-1ExampleStructureNMRMS(M + H)MS(M âˆ’ H)Note1-1 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 2.50 (3H, s), 7.38 td, J = 8.3, 2.5 Hz), 7.46 (1H, dd, J = 8.4, 5.3 Hz), 7.56-7.65 (1H, br m), 7.95 (2H, d, J = 8.4 Hz), 8.55 (2H, d, J = 8.4 Hz), 13.33 (1H, br s).350348 1-2 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 2.35 (3H, s), 2.50 (3H, s), 7.30 (1H, d, J = 7.7 Hz), 7.34 (1H, d, J = 7.7 Hz), 7.51 (1H, br s), 7.94 (2H, d, J = 8.1 Hz), 8.55 (2H, d, J = 8.1 Hz), 13.17 (1H, br s).346344 1-3 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 2.50 (3H, s), 7.45 (1H, d, J = 8.4 Hz), 7.59 (1H, dd, J = 8.4, 2.3 Hz), 7.81 (1H, br s), 7.94 (2H, d, J = 8.1 Hz), 8.54 (2H, d, J = 8.1 Hz), 13.33 (1H, s).366364 1-4 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 2.38 (3H, s), 7.46 (1H, d, J = 8.1 Hz), 7.55 (1H, d, J = 8.1 Hz), 7.64 (1H, br s), 7.94 (2H, d, J = 8.4 Hz), 8.54 (2H, d, J = 8.4 Hz), 13.49 (1H, br s).366364 1-5 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 7.69 (1H, dd, J = 8.8, 2.2 Hz), 7.83 (1H, d, J = 8.8 Hz), 7.91 (1H, d, J = 2.2 Hz), 7.95 (2H, d, J = 8.2 Hz), 8.53 (2H, d, J = 8.2 Hz), 13.67 (1H, br s).436434 1-6 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 3.83 (3H, s), 7.21 (1H, dd, J = 8.8, 3.1 Hz), 7.41 (1H, d, J = 3.1 Hz), 7.57 (1H, d, J = 8.8 Hz), 7.94 (2H, d, J = 8.4 Hz), 8.54 (2H, d, J = 8.4 Hz), 13.52 (1H, br s).382380 1-7 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 7.01 (1H, dd, J = 8.6, 2.9 Hz), 7.17 (1H, s), 7.44 (1H, d, J = 8.6 Hz), 7.94 (2H, d, J = 8.4 Hz), 8.53 (2H, d, J = 8.4 Hz), 10.17 (1H, s), 13.46 (1H, br s).368366 1-8 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 7.81 (1H, d, J = 8.4 Hz), 7.94 (2H, d, J = 8.4 Hz), 8.12 (1H, dd, J = 8.5, 2.0 Hz), 8.36 (1H, d, J = 1.9 Hz), 8.54 (2H, d, J = 8.4 Hz), 13.53 (2H, br s).396394

TABLE 1-21-9 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 2.63 (3H, s), 7.66 (1H, d, J = 8.2 Hz), 7.88 (1H, dd, J = 8.3, 1.7 Hz), 7.95 (2H, d, J = 8.4 Hz), 8.11 (1H, br s), 8.54 (2H, d, J = 8.4 Hz), 13.41 (1H, br s).400398 1-10 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 2.61 (3H, s), 7.54 (1H, d, J = 7.9 Hz), 7.94 (2H, d, J = 8.4 Hz), 8.04 (1H, dd, J = 7.9, 1.8 Hz), 8.30 (1H, br s), 8.55 (2H, d, J = 8.4 Hz), 13.23 (2H, br s).376374 1-11 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 2.59 (3H, s), 7.46 (1H, br s), 7.50 (1H, d, J = 8.2 Hz), 7.94 (2H, d, J = 8.4 Hz), 8.00 (1H, dd, J = 7.9, 1.8 Hz), 8.01 (1H, br s), 8.24 (1H, br s), 8.56 (2H, d, J = 8.4 Hz), 13.28 (1H, s).375373 1-12 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 4.58 (2H, d, J = 5.5 Hz), 5.45 (1H, t, J = 5.6 Hz), 7.56 (1H, dd, J = 8.2, 2.0 Hz), 7.62 (1H, d, J = 8.2 Hz), 7.74 (1H, br s), 7.94 (2H, d, J = 8.4 Hz), 8.54 (2H, d, J = 8.4 Hz), 13.53 (1H, br s).382380 1-13 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 2.65 (3H, s), 7.84 (1H, d, J = 8.4 Hz), 7.95 (2H, d, J = 8.4 Hz), 8.16 (1H, dd, J = 8.4, 2.2 Hz), 8.38 (1H, d, J = 2.2 Hz), 8.54 (2H, d, J = 8.4 Hz), 13.65 (1H, s).394392 1-14 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.22 (3H, t, J = 7.7 Hz), 2.50 (3H, s), 2.66 (2H, q, J = 7.6 Hz), 7.32 (1H, d, J = 7.9 Hz), 7.37 (1H, dd, J = 7.8, 1.7 Hz), 7.55 (1H, br s), 7.94 (2H, d, J = 8.4 Hz), 8.55 (2H, d, J = 8.4 Hz), 13.19 (1H, br s).360358 1-15 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.39 (9H, s), 4.20 (2H, d, J = 6.0 Hz), 7.47-7.53 (2H, m), 7.62 (1H, d, J = 8.4 Hz), 7.66 (1H, br s), 7.93 (2H, d, J = 8.4 Hz), 8.53 (2H, d, J = 8.4 Hz), 13.54 (1H, s).481479 1-16 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.89 (3H, s), 4.32 (2H, d, J = 6.0 Hz), 7.49 (1H, dd, J = 8.3, 2.3 Hz), 7.62 (1H, d, J = 8.2 Hz), 7.67 (1H, d, J = 2.2 Hz), 7.94 (2H, d, J = 8.4 Hz), 8.46 (1H, t, J = 6.0 Hz), 8.53 (2H, d, J = 8.4 Hz), 13.55 (1H, br s).423421

TABLE 1-31-17 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 4.13 (2H, br s), 7.72- 7.77 (2H, m), 7.93-7.97 (3H, m), 8.39 (3H, br s), 8.54 (2H, d, J = 8.4 Hz), 13.67 (1H, s).381379 1-18 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.36 (3H, d, J = 6.4 Hz), 4.78-4.84 (1H, m), 5.42 (1H, d, J = 4.2 Hz), 7.57-7.62 (2H, m), 7.77 (1H, br s), 7.94 (2H, d, J = 8.2 Hz), 8.54 (2H, d, J = 8.2 Hz), 13.52 (1H, br s).396394 1-19 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 3.34 (3H, s), 4.50 (2H, s), 7.57 (1H, dd, J = 8.3, 2.1 Hz), 7.65 (1H, d, J = 8.2 Hz), 7.76 (1H, d, J = 2.0 Hz), 7.94 (2H, d, J = 8.4 Hz), 8.54 (2H d, J = 8.4 Hz), 13.54 (1H, br s).396394 1-20 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 2.93 (3H, s), 4.25 (2H, d, J = 6.4 Hz), 7.60 (1H, dd, J = 8.4, 2.0 Hz), 7.66 (1H, d, J = 8.4 Hz), 7.70 (1H, t, J = 6.4 Hz), 7.78 (1H, d, J = 2.0 Hz), 7.94 (2H, d, J = 8.4 Hz), 8.54 (2H, d, J = 8.4 Hz), 13.58 (1H, br s).459457 1-21 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 2.07 (1.0H, s), 2.08 (2.0H, s), 2.82 (1.0H, s), 2.96 (2.0H, s), 4.56 (1.3H, s), 4.64 (0.7H, s), 7.47 (1.0H, d, J = 8.2 Hz), 7.61-7.68 (2.0H, m), 7.94 (2.0H, d, J = 8.4 Hz), 8.53 (2.0H, d, J = 8.4 Hz), 13.56 (1.0H, s).437435 1-22 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 3.56 (3H, s), 4.26 (2H, d, J = 6.2 Hz), 7.50 (1H, dd, J = 8.5, 2.1 Hz), 7.63 (1H, d, J = 8.4 Hz), 7.68 (1H, d, J = 2.0 Hz), 7.80 (1H, t, J = 6.2 Hz), 7.94 (2H, d, J = 8.4 Hz), 8.53 (2H, d, J = 8.4 Hz), 13.54 (1H, s).439437 1-23 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 3.71 (2H, s), 7.53 (1H, dd, J = 8.3, 1.9 Hz), 7.62 (1H, d, J = 8.2 Hz), 7.71 (1H, d, J = 1.8 Hz), 7.94 (2H, d, J = 8.4 Hz), 8.54 (2H, d, J = 8.4 Hz), 12.53 (1H, br s), 13.54 (1H, br s).410408 1-24 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.13 (9H, s), 4.32 (2H, d, J = 6.0 Hz), 7.45 (1H, dd, J = 8.3, 2.1 Hz), 7.61 (1H, d, J = 8.2 Hz), 7.65 (1H, d, J = 2.0 Hz), 7.94 (2H, d, J = 8.4 Hz), 8.18 (1H, t, J = 6.1 Hz), 8.53 (2H, d, J = 8.4 Hz), 13.55 (1H, br s).465463

TABLE 1-41-25 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 2.09 (3H, s), 5.16 (2H, s), 7.63 (1H, dd, J = 8.3, 2.1 Hz), 7.68 (1H, d, J = 8.2 Hz), 7.81 (1H, d, J = 2.0 Hz), 7.94 (2H, d, J = 8.4 Hz), 8.54 (2H, d, J = 8.4 Hz), 13.57 (1H, br s).424422 1-26 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 2.59 (3H, d, J = 4.6 Hz), 3.50 (2H, s), 7.50 (1H, dd, J = 8.3, 2.1 Hz), 7.60 (1H, d, J = 8.4 Hz), 7.67 (1H, d, J = 2.0 Hz). 7.94 (2H, d, J = 8.4 Hz), 8.01-8.06 (1H, br m), 8.54 (2H, d, J = 8.4 Hz), 13.54 (1H, s).423421 1-27 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.02 (3H, t, J = 7.6 Hz), 2.16 (2H, q, J = 7.6 Hz), 4.32 (2H, d, J = 6.0 Hz), 7.49 (1H, dd, J = 8.4, 2.2 Hz), 7.62 (1H, d, J = 8.4 Hz), 7.67 (1H, d, J = 2.2 Hz), 7.94 (2H, d, J = 8.4 Hz), 8.39 (1H, t, J = 6.0 Hz), 8.53 (2H, d, J = 8.4 Hz), 13.55 (1H, br s).437435 1-28 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.04 (6H, d, J = 7.1 Hz), 2.38-2.48 (1H, m), 4.32 (2H, d, J = 6.2 Hz), 7.47 (1H, dd, J = 8.4, 2.2 Hz), 7.62 (1H, d, J = 8.4 Hz), 7.66 (1H, d, J = 2.2 Hz), 7.94 (2H, d, J = 8.4 Hz), 8.37 (1H, t, J = 6.2 Hz), 8.53 (2H, d, J = 8.4 Hz), 13.55 (1H, br s).451449 1-29 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.11-1.40 (5H, m), 4.67-1.76 (5H, m), 2.13-2.20 (1H, m), 4.31 (2H, d, J = 6.2 Hz), 7.46 (1H, dd, J = 8.3, 2.1 Hz), 7.61 (1H, d, J = 8.2 Hz), 7.65 (1H, d, J = 2.0 Hz), 7.93 (2H, d, J = 8.4 Hz), 8.34 (1H, t, J = 6.1 Hz), 8.53 (2H, d, J = 8.4 Hz), 13.55 (1H, br s).491489 1-30 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.44-1.82 (8H, m), 2.58-2.66 (1H, m), 4.32 (2H, d, J = 6.2 Hz), 7.47 (1H, dd, J = 8.3, 2.1 Hz), 7.62 (1H, d, J = 8.2 Hz), 7.66 (1H, d, J = 2.0 Hz), 7.93 (2H, d, J = 8.4 Hz), 8.39 (1H, t, J = 6.2 Hz), 8.53 (2H, d, J = 8.4 Hz), 13.55 (1H, br s).477475 1-31 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.20 (3H, t, J = 7.3 Hz), 3.02 (2H, q, J = 7.4 Hz), 4.23 (2H, d, J = 6.5 Hz), 7.60 (1H, dd, J = 8.4, 2.1 Hz), 7.66 (1H, d, J = 8.4 Hz), 7.72 (1H, t, J = 6.4 Hz), 7.77 (1H, d, J = 2.1 Hz), 7.94 (2H, d, J = 8.4 Hz), 8.54 (2H, d, J = 8.4 Hz), 13.57 (1H, br s).473471 1-32 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.31 (9H, s), 4.32 (2H, d, J = 6.4 Hz), 7.60-7.66 (3H, m), 7.76 (1H, br s), 7.94 (2H, d, J = 8.4 Hz), 8.53 (2H, d, J = 8.4 Hz), 13.58 (1H, br s).501499

TABLE 1-51-33 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.24 (6H, d, J = 6.8 Hz), 3.14-3.20 (1H, m), 4.25 (2H, d, J = 6.4 Hz), 7.60 (1H, dd, J = 8.5, 1.9 Hz), 7.66 (1H, d, J = 8.4 Hz), 7.70 (1H, t, J = 6.5 Hz), 7.77 (1H, d, J = 1.8 Hz), 7.94 (2H, d, J = 8.2 Hz), 8.53 (2H, d, J = 8.2 Hz), 13.58 (1H, br s).487485 1-34 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 2.81 (6H, s), 4.28 (2H, d, J = 5.8 Hz), 6.98 (1H, t, J = 5.9 Hz), 7.50 (1H, dd, J = 8.3, 2.0 Hz), 7.59 (1H, d, J = 8.4 Hz), 7.68 (1H, d, J = 1.9 Hz), 7.94 (2H, d, J = 8.4 Hz), 8.53 (2H, d, J = 8.4 Hz), 13.54 (1H, br s).452450 1-35 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 2.56 (3H, d, J = 4.6 Hz), 4.26 (2H, d, J = 6.2 Hz), 5.90 (1H, q, J = 4.6 Hz), 6.52 (1H, t, J = 6.2 Hz), 7.49 (1H, dd, J = 8.3, 2.1 Hz), 7.60 (1H, d, J = 8.4 Hz), 7.66 (1H, d, J = 2.0 Hz), 7.94 (2H, d, J = 8.4 Hz), 8.53 (2H, d, J = 8.4 Hz), 13.55 (1H, br s).438436 1-36 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.85 (3H, t, J = 7.4 Hz), 1.54 (2H, sextet, J = 7.4 Hz), 2.13 (2H, t, J = 7.4 Hz), 4.33 (2H, d, J = 6.2 Hz), 7.48 (1H, dd, J = 8.2, 2.0 Hz), 7.62 (1H, d, J = 8.2 Hz), 7.66 (1H, d, J = 2.0 Hz), 7.94 (2H, d, J = 8.2 Hz), 8.41 (1H, t, J = 6.1 Hz), 8.53 (2H, d, J = 8.2 Hz), 13.55 (1H, br s).451449 1-37 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.87 (6H, d, J = 6.4 Hz), 1.96-2.06 (3H, m), 4.33 (2H, d, J = 6.0 Hz), 7.48 (1H, dd, J = 8.2, 2.0 Hz), 7.61 (1H, d, J = 8.2 Hz), 7.66 (1H, d, J = 2.0 Hz), 7.93 (2H, d, J = 8.4 Hz), 8.42 (1H, t, J = 6.1 Hz), 8.53 (2H, d, J = 8.4 Hz), 13.55 (1H, br s).465463 1-38 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.16 (3H, t, J = 7.1 Hz), 4.01 (2H, q, J = 7.1 Hz), 4.25 (2H, d, J = 6.2 Hz), 7.50 (1H, dd, J = 8.4, 2.0 Hz), 7.63 (1H, d, J = 8.4 Hz), 7.68 (1H, d, J = 2.0 Hz), 7.76 (1H, t, J = 6.3 Hz), 7.94 (2H, d, J = 8.4 Hz), 8.53 (2H, d, J = 8.4 Hz), 13.55 (1H, br s).453451 1-39 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.17 (6H, d, J = 6.2 Hz), 4.24 (2H, d, J = 6.2 Hz), 4.73-4.80 (1H, m), 7.49 (1H, dd, J = 8.4, 2.0 Hz), 7.63 (1H, d, J = 8.4 Hz), 7.66-7.71 (2H, m), 7.93 (2H, d, J = 8.4 Hz), 8.53 (2H, d, J = 8.4 Hz), 13.55 (1H, br s).467465 1-40 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 2.85 (3H, s), 3.05 (3H, s), 3.80 (2H, s), 7.47 (1H, dd, J = 8.4, 2.2 Hz), 7.59 (1H, d, J = 8.4 Hz), 7.65 (1H, d, J = 2.2 Hz), 7.94 (2H, d, J = 8.4 Hz), 8.54 (2H, d, J = 8.4 Hz), 13.54 (1H, br s).437435

TABLE 1-61-41 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.25 (9H, s), 3.47 (2H, s), 7.49 (1H, dd, J = 8.5, 2.1 Hz), 7.59 (1H, d, J = 8.4 Hz), 7.66 (1H, d, J = 2.0 Hz), 7.77 (1H, s), 7.94 (2H, d, J = 8.4 Hz), 8.54 (2H, d, J = 8.4 Hz), 13.54 (1H, s). 465463 1-42 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.51-1.71 (4H, m), 1.83-1.92 (4H, m), 3.47-3.54 (1H, m), 4.26 (2H, d, J = 6.4 Hz), 7.59 (1H, d, J = 8.3 Hz), 7.66 (1H, d, J = 8.3 Hz), 7.72 (1H, t, J = 6.3 Hz), 7.77 (1H, s), 7.94 (2H, d, J = 8.3 Hz), 8.53 (2H, d, J = 8.3 Hz), 13.58 (1H, br s).513511 1-43 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.13 (9H, s), 4.31 (2H, d, J = 6.0 Hz), 7.44 (1H, dd, J = 8.2, 2.0 Hz), 7.56-7.58 (3H, m), 7.64-7.69 (2H, m), 8.18 (1H, t, J = 6.0 Hz), 8.34 (2H, d, J = 7.1 Hz), 13.34 (1H, br s).397395 1-44 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.91 (3H, t, J = 7.4 Hz), 1.13 (9H, s), 1.62-1.65 (2H, m), 2.65 (2H, t, J = 7.5 Hz), 4.31 (2H, d, J = 6.0 Hz), 7.38 (2H, d, J = 8.2 Hz), 7.43 (1H, dd, J = 8.4, 2.0 Hz), 7.59 (1H, d, J = 8.4 Hz), 7.64 (1H, br s), 8.17 (1H, t, J = 6.1 Hz), 8.26 (2H, d, J = 8.2 Hz), 13.25 (1H, br s).439437 1-45 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 2.51 (3H, s), 4.55 (2H, d, J = 5.5 Hz), 5.29 (1H, t, J = 5.6 Hz), 7.36 (1H, d, J = 7.9 Hz), 7.46 (1H, dd, J = 7.8, 1.7 Hz), 7.65 (1H, br s), 7.94 (2H, d, J = 8.4 Hz), 8.55 (2H, d, J = 8.4 Hz), 13.22 (1H, br s).362360 1-46 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.13 (9H, s), 1.24 (6H, d, J = 6.8 Hz), 2.96-3.03 (1H, m), 4.31 (2H, d, J = 6.0 Hz), 7.41-7.46 (3H, m), 7.58 (1H, d, J = 8.4 Hz), 7.64 (1H, br s), 8.17 (1H, t, J = 6.0 Hz), 8.27 (2H, d, J = 8.2 Hz), 13.26 (1H, br s).439437 1-47 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.88 (3H, s), 2.50 (3H, s), 4.29 (2H, d, J = 6.0 Hz), 7.35 (1H, d, J = 7.9 Hz), 7.39 (1H, dd, J = 7.9, 1.8 Hz), 7.59 (1H, br s), 7.94 (2H, d, J = 8.2 Hz), 8.37 (1H, t, J = 6.0 Hz), 8.54 (2H, d, J = 8.2 Hz), 13.26 (1H, br s).403401 1-48 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.13 (9H, s), 2.50 (3H, s), 4.30 (2H, d, J = 6.0 Hz), 7.34-7.35 (2H, br m), 7.57 (1H, br s), 7.93 (2H, d, J = 8.4 Hz), 8.09 (1H, t, J = 6.1 Hz), 8.54 (2H, d, J = 8.4 Hz), 13.24 (1H, br s).445443

TABLE 1-71-49 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.13 (9H, s), 1.25 (6H, d, J = 7.0 Hz), 2.96-3.03 (1H, m), 4.32 (2H, d, J = 5.8 Hz), 7.44 (1H, dd, J = 8.1, 1.2 Hz), 7.48 (1H, t, J = 7.7 Hz), 7.56 (1H, d, J = 7.7 Hz), 7.59 (1H, d, J = 8.1 Hz), 7.65 (1H, br s), 8.16-8.20 (3H, m), 13.30 (1H, br s).439437 1-50 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.91 (3H, t, J = 7.3 Hz), 1.13 (9H, s), 1.61-1.65 (2H, m), 2.65 (2H, t, J = 7.5 Hz), 4.31 (2H, d, J = 6.2 Hz), 7.43-7.50 (3H, m), 7.59 (1H, d, J = 8.2 Hz), 7.64 (1H, br s), 8.16-8.18 (3H, m), 13.30 (1H, s).439437 1-51 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.88 (6H, d, J = 6.6 Hz), 1.13 (9H, s), 1.88-1.92 (1H, m), 2.55 (2H d, J = 7.1 Hz), 4.31 (2H, d, J = 6.2 Hz), 7.35 (2H, d, J = 8.2 Hz), 7.43 (1H, dd, J = 8.4, 2.0 Hz), 7.58 (1H, d, J = 8.2 Hz), 7.64 (1H, br s), 8.17 (1H, t, J = 6.1 Hz), 8.26 (2H, d, J = 8.4 Hz), 13.25 (1H, br s).439437 1-52 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.06 (3H, s), 1.14- 1.49 (8H, m), 1.91-1.98 (2H, m), 4.32 (2H, d, J = 5.8 Hz), 7.43 (1H, d, J = 8.1 Hz), 7.52- 7.59 (3H, m), 7.62-7.67 (2H, m), 8.16 (1H, t, J = 5.9 Hz), 8.32 (2H, d, J = 8.1 Hz), 13.31 (1H, br s).437435 1-53 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.26 (6H, s), 3.15 (3H, s), 4.32 (2H, d, J = 6.3 Hz), 7.45 (1H, d, J = 7.2 Hz), 7.53-7.59 (3H, m), 7.63-7.68 (2H, m), 8.32 (2H, d, J = 7.9 Hz), 8.46 (1H, t, J = 6.3 Hz), 13.31 (1H, br s).413411 1-54 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.12 (9H, s), 4.31 (2H, d, J = 6.0 Hz), 7.45 (1H, d, J = 8.4 Hz), 7.60 (1H, d, J = 8.4 Hz), 7.63 (1H, s), 7.81 (1H, t, J = 7.9 Hz), 8.03 (1H, d, J = 7.4 Hz), 8.16 (1H, t, J = 5.9 Hz), 8.57-8.63 (2H, m), 13.51 (1H, br s).465463 1-55 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.13 (9H, s), 1.22 (3H, t, J = 7.6 Hz), 2.70 (2H, q, J = 7.6 Hz), 4.31 (2H. d. J = 6.0 Hz), 7.42-7.53 (3H, m), 7.59 (1H, d, J = 8.2 Hz), 7.64 (1H, s), 8.15- 8.19 (3H, m), 13.31 (1H, br s).425423 1-56 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.28 (6H, s), 3.17 (3H, s), 4.33 (2H, d, J = 6.2 Hz), 7.48 (1H, dd, J = 8.4, 2.1 Hz), 7.61 (1H, d, J = 8.3 Hz), 7.67 (1H, s), 7.94 (2H, d, J = 8.3 Hz), 8.48 (1H, t, J = 6.3 Hz), 8.52 (2H, d, J = 8.3 Hz), 13.55 (1H, br s).481479

TABLE 1-81-57 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.13 (9H, s), 1.34 (9H, s), 4.31 (2H, d, J = 6.0 Hz), 7.43 (1H, dd, J = 8.2, 2.0 Hz), 7.49 (1H, t, J = 7.8 Hz), 7.59 (1H, d, J = 8.2 Hz), 7.66 (1H, s), 7.72 (1H, d, J = 8.2 Hz), 8.15-8.18 (2H, m), 8.38 (1H, s), 13.33 (1H, br s).453451 1-58 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.72 (3H, t, J = 7.4 Hz), 1.08 (6H, s), 1.49 (2H, q, J = 7.4 Hz), 4.32 (2H, d, J = 6.0 Hz), 7.47 (1H, dd, J = 8.2, 2.0 Hz), 7.61 (1H, d, J = 8.2 Hz), 7.65 (1H, s), 7.82 (1H, t, J = 7.8 Hz), 8.04 (1H, d, J = 7.9 Hz), 8.15 (1H, t, J = 6.1 Hz), 8.59-8.63 (2H, m), 13.54 (1H, s).479477 1-59 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.37 (6H, s), 4.36 (2H, d, J = 5.6 Hz), 7.45 (1H, d, J = 8.4 Hz), 7.62 (1H, d, J = 8.4 Hz), 7.64 (1H, s), 7.81 (1H, t, J = 7.7 Hz), 8.03 (1H, d, J = 7.9 Hz), 8.58-8.66 (3H, m), 13.53 (1H, br s).519517 1-60 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.13 (9H, s), 4.31 (2H, d, J = 6.0 Hz), 7.45 (1H, d, J = 8.4 Hz), 7.55 (2H, d, J = 8.7 Hz), 7.61 (1H, d, J = 8.4 Hz), 7.64 (1H, s), 8.18 (1H, t, J = 6.0 Hz), 8.46 (2H, d, J = 8.7 Hz), 13.45 (1H, br s).481479 1-61 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.13 (9H, s), 4.31 (2H, d, J = 6.0 Hz), 7.45 (1H, dd, J = 8.4, 2.2 Hz), 7.60 (1H, d, J = 8.4 Hz), 7.63-7.73 (3H, m), 8.18 (1H, t, J = 6.0 Hz), 8.21 (1H, s), 8.37 (1H, dt, J = 7.4, 1.5 Hz), 13.50 (1H, br s).481479 1-62 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.12 (9H, s), 1.34 (3H, t, J = 7.0 Hz), 4.09 (2H, q, J = 7.0 Hz), 4.30 (2H, d, J = 6.0 Hz), 7.20 (1H, dd, J = 8.1, 2.3 Hz), 7.41-7.46 (2H, m), 7.57 (1H, d, J = 8.4 Hz), 7.63 (1H, s), 7.84 (1H, s), 7.91 (1H, d, J = 7.7 Hz), 8.16 (1H, t, J = 6.0 Hz), 13.30 (1H, br s).441439 1-63 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.13 (9H, s), 2.41 (3H, s), 4.31 (2H, d, J = 6.0 Hz), 7.37 (2H, d, J = 8.1 Hz), 7.43 (1H, d, J = 8.1 Hz), 7.59 (1H, d, J = 8.1 Hz), 7.64 (1H, s), 8.17 (1H, t, J = 6.0 Hz), 8.24 (2H, d, J = 8.1 Hz), 13.24 (1H, br s).411409 1-64 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.14 (9H, s), 2.40 (3H, s), 4.32 (2H, d, J = 6.0 Hz), 7.41-7.51 (3H, m), 7.60 (1H, d, J = 8.4 Hz), 7.64 (1H, s), 8.11-8.20 (3H, m), 13.30 (1H, br s).411409

TABLE 1-91-65 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.60-1.71 (6H, m), 1.79-1.83 (6H, m), 1.94-1.98 (3H, m), 4.30 (2H, d, J = 6.0 Hz), 7.42 (1H, d, J = 8.6 Hz), 7.53-7.59 (3H, m), 7.63-7.68 (2H, m), 8.10 (1H, t, J = 6.1 Hz), 8.34 (2H, d, J = 7.5 Hz), 13.34 (1H, br s).475473 1-66 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 172-1.94 (2H, m), 1.99-2.08 (2H, m), 2.10-2.20 (2H, m), 3.03- 3.11 (1H, m), 4.31 (2H, d, J = 6.0 Hz), 7.45 (1H, dd, J = 8.4, 2.0 Hz), 7.54-7.69 (5H, m), 8.28 (1H, t, J = 6.0 Hz), 8.34 (2H, d, J = 7.3 Hz), 13.34 (1H, br s).395393 1-67 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.46-1.81 (8H, m), 2.58-2.66 (1H, m), 4.32 (2H, d, J = 6.0 Hz), 7.45 (1H, dd, J = 8.4, 2.2 Hz), 7.56 (2H, t, J = 7.6 Hz), 7.60 (1H, d, J = 8.2 Hz), 7.64-7.69 (2H, m), 8.34 (2H, d, J = 7.5 Hz), 8.40 (1H, t, J = 6.1 Hz), 13.34 (1H, br s).409407 1-68 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.50-1.68 (4H, m), 1.82-1.91 (2H, m), 2.29-2.36 (2H, m), 4.37 (2H, d, J = 5.8 Hz), 7.41 (1H, d, J= 8.8 Hz), 7.54 (2H, t, J = 7.7 Hz), 7.59 (1H, d, J = 8.4 Hz), 7.62-7.67 (2H, m), 8.32 (2H, d, J = 7.7 Hz), 8.69 (1H, t, J = 5.9 Hz), 13.33 (1H, br s).477475 1-69 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.38 (6H, s), 4.37 (2H, d, J = 6.0 Hz), 7.44 (1H, dd, J = 8.3, 2.1 Hz), 7.56 (2H, t, J = 7.6 Hz), 7.61 (1H, d, J = 8.4 Hz), 7.64-7.69 (2H, m), 8.34 (2H, d, J = 7.5 Hz), 8.64 (1H, t, J = 6.0 Hz), 13.35 (1H, br s).451449 1-70 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.77-1.94 (2H, m), 2.30-2.39 (2H, m), 2.45-2.57 (2H, m), 4.39 (2H, d, J = 5.8 Hz), 7.44 (1H, d, J = 7.4 Hz), 7.54 (2H, t, J = 7.7 Hz), 7.60 (1H, d, J = 8.4 Hz), 7.63-7.68 (2H, m), 8.33 (2H, d, J = 7.7 Hz), 8.78 (1H, t, J = 5.9 Hz), 13.32 (1H, br s).463461 1-71 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.08-1.42 (5H, m), 1.57-1.77 (5H m), 2.13-2.21 (1H, m), 4.31 (2H, d, J = 6.0 Hz), 7.44 (1H dd, J = 8.2, 2.2 Hz), 7.54-7.60 (3H, m), 7.65-7.69 (2H, m), 8.33-8.35 (3H, m), 13.34 (1H, br s).423421 1-72 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.35 (3H, s), 1.63- 1.76 (3H, m), 1.84-1.93 (1H, m), 2.30-2.37 (2H, m), 4.32 (2H, d, J = 6.2 Hz), 7.45 (1H, dd, J = 8.4, 2.0 Hz), 7.56 (2H, t, J = 7.6 Hz), 7.60 (1H, d, J = 8.4 Hz), 7.64-7.68 (2H, m), 8.18 (1H, t, J = 6.1 Hz), 8.34 (2H, d, J = 7.5 Hz), 13.35 (1H, br s).409407

TABLE 1-101-73 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.19 (3H, s), 1.35- 1.43 (2H, m), 1.51-1.62 (4H, m), 1.98-2.05 (2H, m), 4.32 (2H, d, J = 6.0 Hz), 7.44 (1H, dd, J = 8.3, 2.1 Hz), 7.54-7.60 (3H m), 7.64- 7.69 (2H, m), 8.20 (1H, t, J = 6.1 Hz), 8.34 (2H, d, J = 7.3 Hz), 13.34 (1H, br s).423421 1-74 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.12 (9H, s), 4.30 (2H, d, J = 6.0 Hz), 7.37 (1H, d, J = 8.8 Hz), 7.39 (1H, d, J = 8.8 Hz), 7.42 (1H, dd, J = 8.5, 2.0 Hz), 7.58 (1H, d, J = 8.4 Hz), 7.62 (1H, s), 8.16 (1H, t, J = 5.9 Hz), 8.38 (1H, d, J = 8.8 Hz), 8.40 (1H, d, J = 8.8 Hz), 13.33 (1H, br s).415413 1-75 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.13 (9H, s), 3.32 (3H, s), 4.31 (2H, d, J = 6.0 Hz), 4.50 (2H, s), 7.43 (1H, d, J = 8.6 Hz), 7.53 (1H, t, J = 7.7 Hz), 7.59 (2H, d, J = 8.1 Hz), 7.63 (1H, s), 8.18 (1H, t, J = 6.0 Hz), 8.26 (1H, d, J = 7.7 Hz), 8.30 (1H, s), 13.34 (1H, br s).441439 1-76 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.14 (9H, s), 4.32 (2H, d, J = 6.0 Hz), 7.45 (1H, dd, J = 8.4, 2.1 Hz), 7.52 (1H, td, J = 8.4, 2.7 Hz), 7.59-7.63 (2H, m), 7.65 (1H, d, J = 2.1 Hz), 8.05 (1H, dt, J = 9.8, 2.0 Hz), 8.16-8.20 (2H, m), 13.43 (1H, br s).415413 1-77 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.38 (6H, s), 4.37 (2H, d, J = 5.8 Hz), 7.41 (1H, d, J = 8.4 Hz), 7.58 (1H, d, J = 8.4 Hz), 7.61-7.65 (2H, m), 7.69 (1H, t, J = 7.8 Hz), 8.21 (1H, s), 8.36 (1H, d, J = 7.7 Hz), 8.63 (1H, t, J = 5.8 Hz), 13.52 (1H, br s).535533 1-78 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.48-1.71 (4H, m), 1.81-1.94 (2H, m), 2.30-2.39 (2H, m), 4.39 (2H, d, J = 5.9 Hz), 7.45 (1H, dd, J = 8.3, 1.4 Hz), 7.59-7.75 (4H, m), 8.22 (1H, br s), 8.35- 8.39 (1H, m), 8.72 (1H, t, J = 5.9 Hz), 13.51 (1H, br s).561559 1-79 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.50-1.69 (4H, m), 1.83-1.93 (2H, m), 2.29-2.39 (2H, m), 4.39 (2H, d, J = 6.0 Hz), 7.44 (1H, dd, J = 8.3, 1.9 Hz), 7.60-7.66 (2H, m), 7.82 (1H, t, J = 7.7 Hz), 8.03 (1H, d, J = 77 Hz), 8.59-8.65 (2H, m), 8.71 (1H, t, J = 6.0 Hz), 13.54 (1H, br s).545543 1-80 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.34 (9H, s), 1.49- 1.69 (4H, m), 1.83-1.92 (2H, m), 2.29-2.38 (2H, m), 4.38 (2H, d, J = 6.0 Hz), 7.42 (1H, d, J = 8.3 Hz), 7.49 (1H, t, J = 7.8 Hz), 7.61 (1H, d, J = 8.3 Hz), 7.65 (1H, s), 7.71 (1H, d, J = 7.8 Hz), 8.15 (1H, d, J = 7.8 Hz), 8.38 (1H, s), 8.71 (1H, t, J = 6.0 Hz), 13.35 (1H, br s).533531

TABLE 1-111-81 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.37 (6H, s), 2.32 (3H, d, J = 1.2 Hz), 4.36 (2H, d, J = 5.9 Hz), 7.50-7.43 (2H, m), 7.60 (1H, d, J = 8.4 Hz), 7.64 (1H, br s), 7.99 (1H, d, J = 10.9 Hz), 8.08 (1H, dd, J = 7.8, 1.5 Hz), 8.63 (1H, t, J = 5.9 Hz), 13.36 (1H, s).483481 1-82 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.13 (9H, s), 1.31 (6H, d, J = 6.0 Hz), 4.31 (2H, d, J = 6.0 Hz), 4.72-4.82 (1H, m), 7.06 (2H, d, J = 8.9 Hz), 7.42 (1H, d, J = 8.4 Hz), 7.58 (1H, d, J = 8.4 Hz), 7.63 (1H, s), 8.17 (1H, t, J = 6.0 Hz), 8.29 (2H, d, J = 8.9 Hz), 13.12 (1H, br s).455453 1-83 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.17 (6H, d, J = 6.3 Hz), 1.34 (9H, s), 4.23 (2H, d, J = 6.0 Hz), 4.72-4.80 (1H, m), 7.43-7.52 (2H, m), 7.60 (1H, d, J = 8.4 Hz), 7.65-7.73 (3H, m), 8.15 (1H, d, J = 7.7 Hz), 8.38 (1H, s), 13.33 (1H, br s).455453 1-84 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.27 (6H, s), 1.34 (9H, s), 3.16 (3H, s), 4.33 (2H, d, J = 6.3 Hz), 7.42-7.46 (1H, m), 7.49 (1H, d, J = 7.9 Hz), 7.57 (1H, d, J = 8.1 Hz), 7.66-7.71 (2H, m), 8.14 (1H, d, J = 7.9 Hz), 8.38 (1H, s), 8.47 (1H, t, J = 6.3 Hz), 13.32 (1H, br s).469467 1-85 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.36 (3H, t, J = 7.0 Hz), 1.51-1.69 (4H, m), 1.84-1.91 (2H, m), 2.30-2.38 (2H, m), 4.10 (2H, q, J = 7.0 Hz), 4.38 (2H, d, J = 6.0 Hz), 7.20 (1H, dd, J = 8.3, 2.4 Hz), 7.39-7.43 (1H, m), 7.46 (1H, d, J = 7.9 Hz), 7.59 (1H, d, J = 8.3 Hz), 7.63 (1H, s), 7.85 (1H, d, J = 2.4 Hz), 7.89-7.96 (1H, m), 8.71 (1H, t, J = 6.0 Hz), 13.33 (1H, br s).521519 1-86 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.02 (9H, S), 1.37 (6H, s), 3.73 (2H, s), 4.35 (2H, d, J = 5.8 Hz), 7.08 (2H, d, J = 9.1 Hz), 7.40 (1H, dd, J = 8.3, 2.2 Hz), 7.58 (1H, d, J = 8.3 Hz), 7.62 (1H, d, J = 1.9 Hz), 8.29 (2H, d, J = 9.1 Hz), 8.62 (1H, t, J = 5.8 Hz), 13.13 (1H, s).537535 1-87 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.54 (3H, s), 3.36 (3H, s), 4.32-4.44 (2H, m), 7.46 (1H, d, J = 8.2 Hz), 7.53-7.71 (5H, m), 8.34 (2H, d, J = 7.5 Hz), 9.04 (1H, t, J = 6.3 Hz), 13.35 (1H, br s).467465 1-88 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.00 (6H, d, J = 6.8 Hz), 1.38 (6H, s), 1.99-2.10 (1H, m), 3.83 (2H, d, J = 6.4 Hz), 4.37 (2H, d, J = 6.0 Hz), 7.23 (1H, dd, J = 8.2, 2.6 Hz), 7.41-7.49 (2H, m), 7.61 (1H, d, J = 8.2 Hz), 7.66 (1H, s), 7.84-7.88 (1H, m), 7.93 (1H, d, J = 7.9 Hz), 8.64 (1H, t, J = 6.0 Hz), 13.33 (1H, br s).523521

TABLE 1-121-89 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.54 (3H, s), 3.36 (3H, s), 4.32-4.44 (2H, m), 7.46 (1H, d, J = 8.2 Hz), 7.53-7.71 (5H, m), 8.34 (2H, d, J = 7.5 Hz), 9.04 (1H, t, J = 6.3 Hz), 13.35 (1H, br s).467465 1-90 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.38 (6H, s), 2.31 (3H, s), 4.37 (2H, d, J = 5.8 Hz), 7.31 (1H, t, J = 9.1 Hz), 7.43 (1H, dd, J = 8.4, 2.0 Hz), 7.60 (1H, d, J = 8.4 Hz), 7.63 (1H, s), 8.21 (1H, s), 8.28 (1H, d, J = 7.4 Hz), 8.64 (1H, t, J = 5.8 Hz), 13.32 (1H, br s).483481 1-91 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.30 (6H, d, J = 6.0 Hz), 1.38 (6H, s), 4.37 (2H, d, J = 5.8 Hz), 4.66-4.72 (1H, m), 7.20 (1H, dd, J = 8.4, 2.1 Hz), 7.42-7.47 (2H, m), 7.60 (1H, d, J = 8.4 Hz), 7.66 (1H, s), 7.84-7.86 (1H, m), 7.90 (1H, d, J = 7.7 Hz), 8.64 (1H, t, J = 5.8 Hz), 13.32 (1H, br s).509507 1-92 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.34 (9H, s), 1.38 (6H, s), 4.37 (2H, d, J = 6.0 Hz), 7.42 (1H, d, J = 8.4 Hz), 7.48 (1H, t, J = 7.9 Hz), 7.59 (1H, d, J = 8.4 Hz), 7.66 (1H, s), 7.70 (1H, d, J = 7.7 Hz), 8.15 (1H, d, J = 7.7 Hz), 8.37-8.39 (1H, m), 8.63 (1H, t, J = 6.0 Hz), 13.33 (1H, br s).507505 1-93 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.38 (6H, s), 2.35 (6H, s), 4.37 (2H, d, J = 5.8 Hz), 7.30 (1H, s), 7.44 (1H, d, J = 8.4 Hz), 7.60 (1H, d, J = 8.4 Hz), 7.63 (1H, s), 7.96 (2H, s), 8.64 (1H, t, J = 5.8 Hz), 13.27 (1H, br s).479477 1-94 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.33 (9H, s), 4.55 (2H, d, J = 5.8 Hz), 7.45-7.50 (3H, m), 7.52- 7.56 (2H, m), 7.61 (1H, d, J = 8.4 Hz), 7.71 (1H, d, J = 8.4 Hz), 7.78 (1H, s), 7.89-7.91 (2H, m), 8.14 (1H, d, J = 7.7 Hz), 8.37 (1H, s), 9.15 (1H, t, J = 5.8 Hz), 13.33 (1H, br s).473471 1-95 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.88 (6H, d, J = 6.6 Hz), 1.38 (6H, s), 1.82-1.94 (1H, m), 2.55 (2H, d, J = 7.1 Hz), 4.37 (2H, d, J = 6.0 Hz), 7.42-7.50 (3H, m), 7.59-7.67 (2H, m), 8.12- 8.20 (2H, m), 8.64 (1H, t, J = 6.0 Hz), 13.31 (1H, br s).507505 1-96 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.19 (3H, t, J = 7.1 Hz), 1.54 (3H, s), 3.46-3.54 (1H, m), 3.57- 3.66 (1H, m), 4.40 (2H, d, J = 6.2 Hz), 7.46 (1H, d, J = 8.4 Hz), 7.54-7.70 (5H, m), 8.34 (2H, d, J = 7.5 Hz), 8.86 (1H, t, J = 6.2 Hz), 13.35 (1H, br s).481479

TABLE 1-131-97â€‚ [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.19 (3H, t, J = 7.1 Hz), 1.54 (3H, s), 3.46-3.54 (1H, m), 3.57- 3.66 (1H, m), 4.40 (2H, d, J = 6.2 Hz), 7.46 (1H, d, J = 8.4 Hz), 7.54-7.70 (5H, m), 8.34 (2H, d, J = 7.5 Hz), 8.86 (1H, t, J = 6.2 Hz), 13.35 (1H, br s).481479 1-98â€‚ [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.24 (6H, d, J = 7.0 Hz), 1.38 (6H, s), 4.36 (2H, d, J = 5.8 Hz), 7.35-7.40 (3H, m), 7.54 (1H, d, J = 8.4 Hz), 7.61 (1H, d, J = 2.1 Hz), 8.25 (2H, d, J = 8.4 Hz), 8.62 (1H, t, J = 5.8 Hz), 13.26 (1H, br s).493491 1-99â€‚ [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.38 (6H, s), 4.37 (2H, d, J = 5.8 Hz), 4.86 (2H, q, J = 8.8 Hz), 7.36 (1H, dd, J = 8.0, 2.4 Hz), 7.43 (1H, dd, J = 8.0, 2.4 Hz), 7.53 (1H, t, J = 8.0 Hz), 7.60 (1H, d, J = 8.0 Hz), 7.65 (1H, s), 7.93-7.95 (1H, m), 8.03 (1H, d, J = 7.7 Hz), 8.64 (1H, t, J = 5.8 Hz), 13.35 (1H, br s).549547 1-100 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.36 (6H, s), 1.79- 1.87 (1H, m), 1.93-2.05 (1H, m), 2.07-2.18 (2H, m), 2.29-2.36 (2H, m), 3.55-3.64 (1H, m), 4.35 (2H, d, J = 6.0 Hz), 7.37-7.48 (3H, m), 7.56 (1H, d, J = 8.1 Hz), 7.61 (1H, s), 8.13 (1H, d, J = 7.0 Hz), 8.18 (1H, s), 8.61 (1H, t, J = 6.0 Hz), 13.31 (1H, br s).505503 1-101 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.88 (6H, d, J = 6.5 Hz), 1.25 (9H, s), 1.84-1.95 (1H, m), 2.55 (2H, d, J = 7.2 Hz), 3.45 (2H, s), 7.35 (2H, d, J = 8.1 Hz), 7.46 (1H, d, J = 8.4 Hz), 7.57 (1H, d, J = 8.4 Hz), 7.65 (1H, s), 7.76 (1H, s), 8.26 (2H, d, J = 8.1 Hz), 13.24 (1H, br s).453451 1-102 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.91 (3H, t, J = 7.6 Hz), 1.38 (6H, s), 1.58-1.69 (2H, m), 2.64 (2H, t, J = 7.6 Hz), 4.37 (2H, d, J = 6.0 Hz), 7.40-7.46 (3H, m), 7.58 (1H, d, J = 8.1 Hz), 7.63 (1H, d, J = 1.9 Hz), 8.14-8.18 (2H, m), 8.63 (1H, t, J = 6.0 Hz), 13.32 (1H, br s).493491 1-103 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.54 (3H, s), 3.36 (3H, s), 4.32-4.46 (2H, m), 7.48 (1H, d, J = 7.7 Hz), 7.60-7.75 (4H, m), 8.22 (1H, s), 8.37 (1H, d, J = 7.3 Hz), 9.04 (1H, t, J = 6.3 Hz), 13.52 (1H, br s).551549 1-104 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.91 (3H, t, J = 7.4 Hz), 1.54 (3H, s), 1.58-1.69 (2H, m), 2.65 (2H, t, J = 7.6 Hz), 3.36 (3H, s), 4.32-4.45 (2H, m), 7.43-7.52 (3H, m), 7.61 (1H, d, J = 8.4 Hz), 7.67 (1H, s), 8.13-8.19 (2H, m), 9.04 (1H, t, J = 6.2 Hz), 13.31 (1H, br s).509507

TABLE 1-141-105 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.54 (3H, s), 2.34 (3H, d, J = 1.1 Hz), 3.36 (3H, s), 4.33-4.45 (2H, m), 7.44-7.52 (2H, m), 7.61 (1H, d, J = 8.2 Hz), 7.67 (1H, s), 8.00 (1H, d, J = 11.0 Hz), 8.09 (1H, dd, J = 7.9, 1.5 Hz), 9.04 (1H, t, J = 6.2 Hz), 13.38 (1H, br s).499497 1-106 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.54 (3H, s), 2.32 (3H, d, J = 1.3 Hz), 3.36 (3H, s), 4.32-4.44 (2H, m), 7.32 (1H, t, J = 9.0 Hz), 7.46 (1H, d, J = 8.3 Hz), 7.61 (1H, d, J = 8.3 Hz), 7.65 (1H, s), 8.19-8.25 (1H, m), 8.28 (1H, d, J = 7.3 Hz), 9.03 (1H, t, J = 6.3 Hz), 13.33 (1H, br s).499497 1-107 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.25 (6H, d, J = 6.9 Hz), 1.38 (6H, s), 2.94-3.06 (1H, m), 4.37 (2H, d, J = 5.9 Hz), 7.41-7.68 (5H, m), 8.13- 8.19 (1H, m), 8.22 (1H, br s), 8.64 (1H, t, J = 5.9 Hz), 13.33 (1H, br s).493491 1-108 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.25 (6H, d, J = 7.3 Hz), 1.54 (3H, s), 2.94-3.07 (1H, m), 3.36 (3H, s), 4.31-4.46 (2H, m), 7.42-7.71 (5H, m), 8.16 (1H, d, J = 7.7 Hz), 8.22 (1H, br s), 9.04 (1H, t, J = 6.0 Hz), 13.32 (1H, br s).509507 1-109 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.98 (6H, d, J = 6.5 Hz), 1.37 (6H, s), 2.07-2.00 (1H, m), 3.85 (2H, d, J = 6.5 Hz), 4.35 (2H, d, J = 6.0 Hz), 7.07 (2H, d, J = 9.1 Hz), 7.41 (1H, dd, J = 8.1, 2.1 Hz), 7.58 (1H, d, J = 8.1 Hz), 7.62 (1H, s), 8.29 (2H, d, J = 9.1 Hz), 8.63 (1H, t, J = 5.9 Hz), 13.13 (1H, br s).523521 1-110 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.22 (3H, t, J = 7.6 Hz), 1.38 (6H, s), 2.70 (2H, q, J = 7.6 Hz), 4.37 (2H, d, J = 6.0 Hz), 7.42-7.54 (3H, m), 7.61 (1H, d, J = 8.4 Hz), 7.65 (1H, s), 8.16 (1H, d, J = 7.7 Hz), 8.19 (1H, s), 8.65 (1H, t, J = 6.0 Hz), 13.32 (1H, br s).479477 1-111 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.38 (6H, s), 2.40 (3H, s), 4.37 (2H, d, J = 6.0 Hz), 7.41-7.50 (3H, m), 7.61 (1H, d, J = 8.4 Hz), 7.65 (1H, s), 8.11-8.18 (2H, m), 8.65 (1H, t, J = 6.0 Hz), 13.32 (1H, br s).465463 1-112 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.38 (6H, s), 2.42 (3H, s), 4.37 (2H, d, J = 6.0 Hz), 7.44 (1H, dd, J = 8.4, 2.1 Hz), 7.61 (2H, dd, J = 8.4, 2.1 Hz), 7.64 (1H, d, J = 2.1 Hz), 8.17 (1H, dd, J = 8.4, 2.1 Hz), 8.30 (1H, d, J = 2.1 Hz), 8.65 (1H, t, J = 6.0 Hz), 13.39 (1H, br s).499497

TABLE 1-151-113 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.38 (6H, s), 4.36 (2H, d, J = 6.0 Hz), 4.89 (2H, q, J = 8.8 Hz), 7.20 (2H, d, J = 9.1 Hz), 7.39 (1H, dd, J = 8.3, 2.0 Hz), 7.56 (1H, d, J = 8.3 Hz), 7.62 (1H, d, J = 2.0 Hz), 8.33 (2H, d, J = 9.1 Hz), 8.64 (1H, t, J = 6.0 Hz), 13.24 (1H, br s).549547 1-114 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.94 (6H, d, J = 6.6 Hz), 1.37 (6H, s), 1.63 (2H, q, J = 6.6 Hz), 1.79 (1H, m), 4.04 (2H, t, J = 6.6 Hz), 4.33 (2H, d, J = 6.0 Hz), 6.95 (2H, d, J = 8.9 Hz), 7.23 (1H, d, J = 8.3 Hz), 7.42 (1H, d, J = 8.3 Hz), 7.50 (1H, d, J = 2.0 Hz), 8.22 (2H, d, J = 8.9 Hz), 8.59 (1H, t, J = 6.0 Hz).537535 1-115 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.38 (6H, s), 2.55 (3H, s), 4.36 (2H, d, J = 6.0 Hz), 7.39 (2H, d, J = 8.7 Hz), 7.40 (1H, d, J = 8.3 Hz), 7.58 (1H, d, J = 8.3 Hz), 7.63 (1H, s), 8.26 (2H, d, J = 8.7 Hz), 8.64 (1H, t, J = 6.0 Hz), 13.26 (1H, s).497495 1-116 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.37 (6H, s), 1.94- 1.80 (4H, m), 2.11-2.04 (2H, m), 2.77-2.69 (1H, m), 4.05 (2H, d, J = 6.7 Hz), 4.35 (2H, d, J = 5.8 Hz), 7.07 (2H, d, J = 9.1 Hz), 7.40 (1H, dd, J = 8.4, 2.1 Hz), 7.57 (1H, d, J = 8.4 Hz), 7.62 (1H, s), 8.28 (2H, d, J = 9.1 Hz), 8.62 (1H, t, J = 5.8 Hz), 13.13 (1H, s).535533 1-117 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.27 (6H, d, J = 7.1 Hz), 1.38 (6H, s), 3.19-3.28 (1H, m), 4.37 (2H, d, J = 6.0 Hz), 7.33 (1H, t, J = 9.5 Hz), 7.44 (1H, d, J = 8.4 Hz), 7.59-7.68 (2H, m), 8.21-8.26 (1H, m), 8.33 (1H, dd, J = 7.5, 2.2 Hz), 8.64 (1H, t, J = 6.0 Hz), 13.36 (1H, br s).511509 1-118 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.92 (3H, t, J = 7.3 Hz), 1.38 (6H, s), 1.56-1.67 (2H, m), 2.67 (2H, t, J = 7.7 Hz), 4.37 (2H, d, J = 6.0 Hz), 7.33 (1H, t, J = 9.3 Hz), 7.44 (1H, d, J = 8.4 Hz), 7.59-7.66 (2H, m), 8.21-8.30 (2H, m), 8.65 (1H, t, J = 6.0 Hz), 13.34 (1H, br s).511509 1-119 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.38 (6H, s), 2.01 (3H, t, J = 19.0 Hz), 4.37 (2H, d, J = 5.8 Hz), 7.44 (1H, dd, J = 8.4, 2.1 Hz), 7.61 (2H, d, J = 8.1 Hz), 7.65 (2H, d, J = 2.1 Hz), 7.74 (2H, d, J = 8.4 Hz), 8.43 (2H, d, J = 8.1 Hz), 8.64 (1H, t, J = 5.8 Hz), 13.46 (1H, br s).515513 1-120 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.01 (6H, d, J = 6.7 Hz), 1.37 (6H, s), 2.04-2.10 (1H, m), 3.95 (2H, d, J = 6.7 Hz), 4.33 (2H, d, J = 6.0 Hz), 7.25 (1H, dd, J = 8.3, 2.2 Hz), 7.29 (1H, d, J = 8.3 Hz), 7.44 (1H, d, J = 8.3 Hz), 7.51 (1H, d, J = 2.2 Hz), 8.49-8.53 (2H, m), 8.56-8.62 (1H, m).591589

TABLE 1-161-121 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.23 (3H, t, J = 7.7 Hz), 1.54 (3H, s), 2.70 (2H, q, J = 7.7 Hz), 3.36 (3H, s), 4.32-4.45 (2H, m), 7.44-7.54 (3H, m), 7.61 (1H, d, J = 8.4 Hz), 7.67 (1H, s), 8.13-8.19 (2H, m), 9.03 (1H, t, J = 6.3 Hz), 13.31 (1H, br s).495493 1-122 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.99 (3H, t, J = 7.4 Hz), 1.38 (6H, s), 1.76 (2H, m), 4.04 (2H, t J = 6.6 Hz), 4.36 (2H, d, J = 5.8 Hz), 7.07 (2H, d, J = 8.8 Hz), 7.40 (1H, d, J = 6.0 Hz), 7.57 (1H, d, J = 6.0 Hz), 7.62 (1H, s), 8.29 (2H, d, J = 8.8 Hz), 8.63 (1H, s), 13.14 (1H, s).509507 1-123 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.00 (6H, d, J = 6.9 Hz), 1.54 (3H, s), 1.98-2.12 (1H, m), 3.36 (3H, s), 3.86 (2H, d. J = 6.2 Hz), 4.30-4.45 (2H, m), 7.09 (2H, d, J = 8.5 Hz), 7.44 (1H, d, J = 8.5 Hz), 7.59 (1H, d, J = 8.5 Hz), 7.66 (1H, br s), 8.30 (2H, d, J = 8.9 Hz), 9.03 (1H, t, J = 6.2 Hz), 13.14 (1H, br s).539537 1-124 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.21 (3H, t, J = 7.6 Hz), 1.38 (6H, s), 2.71 (2H, q, J = 7.6 Hz), 4.37 (2H, d, J = 6.0 Hz), 7.33 (1H, t, J = 9.3 Hz), 7.44 (1H, dd, J = 8.5, 2.1 Hz), 7.59-7.66 (2H, m), 8.21-8.26 (1H, m), 8.30 (1H, dd, J = 7.7, 2.2 Hz), 8.65 (1H, t, J = 6.0 Hz), 13.35 (1H, br s).497495 1-125 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.75-2.00 (2H, m), 2.28-2.43 (5H, m), 2.45-2.61 (2H, m), 4.40 (2H, d, J = 5.7 Hz), 7.33 (1H, t, J = 9.1 Hz), 7.46 (1H, dd, J = 8.4, 1.8 Hz), 7.63 (1H, d, J = 8.4 Hz), 7.66 (1H, br s), 8.19-8.32 (2H, m), 8.81 (1H, t, J = 5.7 Hz), 13.33 (1H, br s).495493 1-126 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.54 (3H, s), 3.36 (3H, s), 4.32-4.46 (2H, m), 7.48 (1H, dd, J = 8.4, 1.6 Hz), 7.63 (1H, d, J = 8.4 Hz), 7.68 (1H, br s), 7.94 (2H, d, J = 8.4 Hz), 8.53 (2H, d, J = 8.4 Hz), 9.04 (1H, t, J = 6.0 Hz), 13.56 (1H, br s).535533 1-127 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.20-1.39 (4H, m), 2.32 (3H, s), 4.35 (2H, d, J = 5.9 Hz), 7.33 (1H, t, J = 9.1 Hz), 7.45 (1H, dd, J = 8.4, 1.8 Hz), 7.61 (1H d, J = 8.4 Hz), 7.64 (1H, s), 8.18-8.31 (2H, m), 8.48 (1H, t, J = 5.9 Hz), 13.32 (1H, br s).481479 1-128 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.41 (2H, dd, J = 5.8, 4.0 Hz), 0.55 (2H, dd, J = 5.2, 4.0 Hz), 1.19 (3H, s), 1.38 (6H, s), 3.87 (2H, s), 4.36 (2H, d, J = 5.8 Hz), 7.07 (2H, d, J = 8.9 Hz), 7.41 (1H, d, J = 7.3 Hz), 7.58 (1H, d, J = 7.3 Hz), 7.63 (1H, s), 8.29 (2H, d, J = 8.9 Hz), 8.64 (1H, t, J = 5.8 Hz), 13.14 (1H, s).535533

TABLE 1-171-129 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.39 (6H, s), 2.43 (3H, s), 4.37 (2H, d, J = 6.0 Hz), 7.44 (1H, d, J = 8.4 Hz), 7.55 (1H, d, J = 8.4 Hz), 7.61 (1H, d, J = 8.4 Hz), 7.64 (1H, s), 8.20 (1H, dd, J = 8.4, 1.5 Hz), 8.31 (1H, d, J = 1.5 Hz), 8.64 (1H, t, J = 6.0 Hz), 13.39 (1H, br s).499497 1-130 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.30 (6H, d, J = 6.7 Hz), 1.38 (6H s), 4.37 (2H, d, J = 5.8 Hz), 7.43 (1H, dd, J = 8.4, 2.0 Hz), 7.60 (1H, d, J = 8.4 Hz), 7.65 (1H, d, J = 2.0 Hz), 7.83 (1H, d, J = 8.4 Hz), 8.29 (1H, d, J = 8.4 Hz), 8.52 (1H, s), 8.63 (1H, t, J = 5.8 Hz), 13.55 (1H, br s).561559 1-131 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.94 (3H, t, J = 7.3 Hz), 1.38 (6H, s), 1.45 (2H, m), 1.69-1.76 (2H, m), 3.17 (2H, d, J = 5.1 Hz), 4.08 (2H, m), 4.37 (2H, d, J = 5.8 Hz), 7.10 (2H, d, J = 9.1 Hz), 7.44 (1H, s), 7.60 (m, s), 8.31 (2H, d, J = 9.1 Hz), 8.64 (1H, s), 13.14 (1H, s).523521 1-132 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.35 (3H, t, J = 7.1 Hz), 1.37 (6H, s), 4.13 (2H, q, J = 7.1 Hz), 4.35 (2H, d, J = 6.0 Hz), 7.07 (2H, d, J = 8.8 Hz), 7.42 (aH, brs), 7.60 (2H, brs), 8.29 (2H, d, J = 8.8 Hz), 8.62 (1H, t, J = 6.0 Hz), 13.13 (1H, s).495493 1-133 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.34 (2H, m), 0.57 (2H, m), 1.23 (1H, m), 1.37 (6H, s), 3.92 (2H, d, J = 6.7 Hz), 4.35 (2H, d, J = 6.0 Hz), 7.07 (2H, s), 7.42 (1H, s), 7.59 (2H, s), 8.28 (2H, d, J = 9.1 Hz), 8.62 (1H, t, J = 6.0 Hz), 13.13 (1H, s).521519 1-134 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.38 (6H, s), 4.36 (2H, d, J = 5.8 Hz), 6.90 (2H, d, J = 8.8 Hz), 7.42 (1H, d, J = 8.6 Hz), 7.59 (1H, d, J = 8.6 Hz), 7.64 (1H, s), 8.23 (2H, d, J = 8.8 Hz), 8.64 (1H, t, J = 5.8 Hz), 10.46 (1H, s).467465 1-135 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.74-0.87 (2H, m), 1.01-1.09 (2H, m), 1.38 (6H, s), 2.07-2.15 (1H, m), 4.37 (2H, d, J = 6.0 Hz), 7.33 (1H, t, J = 9.3 Hz), 7.44 (1H, d, J = 8.2 Hz), 7.61 (1H, d, J = 8.2 Hz), 7.65 (1H, s), 7.96 (1H, dd, J = 7.4, 1.9 Hz), 8.15-8.21 (1H, m), 8.64 (1H, t, J = 6.0 Hz), 13.33 (1H, br s).509507 1-136 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.54 (3H, s), 2.42 (3H, s), 3.36 (3H, s), 4.32-4.45 (2H, m), 7.46 (1H, dd, J = 8.4, 2.0 Hz), 7.58-7.68 (3H, m), 8.16 (1H, dd, J = 8.4, 2.0 Hz), 8.30 (1H, d, J = 2.0 Hz), 9.04 (1H, t, J = 6.2 Hz), 13.39 (1H, br s).515513

TABLE 1-181-137 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.99 (3H, t, J = 7.4 Hz), 1.54 (3H, s), 1.71-1.81 (2H, m), 3.36 (3H, s), 4.04 (2H, t, J = 6.6 Hz), 4.32- 4.43 (2H, m), 7.07 (2H, d, J = 9.0 Hz), 7.42 (1H, dd, J = 8.3, 1.9 Hz), 7.58 (1H, d, J = 8.3 Hz), 7.65 (1H, d, J = 1.9 Hz), 8.29 (2H, d, J = 9.0 Hz), 9.03 (1H, t, J = 6.6 Hz), 13.15 (1H, br s).525523 1-138 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.54 (3H, s), 2.01 (3H, t, J = 19.0 Hz), 3.36 (3H, s, J = 8.8 Hz), 4.38 (2H, dd, J = 6.3, 2.8 Hz), 7.44 (1H, dd, J = 8.4, 2.1 Hz), 7.60 (1H, d, J = 8.4 Hz), 7.66 (1H, d, J = 2.1 Hz), 7.73 (2H, d, J = 8.4 Hz), 8.42 (2H, d, J = 8.4 Hz), 9.03 (1H, t, J = 6.3 Hz), 13.47 (1H, s).531529 1-139 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.38 (6H, s), 3.86 (3H, s), 4.37 (2H d, J = 6.0 Hz), 7.10 (2H, d, J = 8.8 Hz), 7.42 (1H, d, J = 8.6 Hz), 7.59 (1H, d, J = 8.6 Hz), 7.64 (1H, s), 8.32 (2H, d, J = 8.8 Hz), 8.63 (1H t, J = 6.0 Hz), 13.15 (1H, s).481479 1-140 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.31 (6H, d, J = 6.0 Hz), 1.38 (6H, s), 4.37 (2H, d, J = 5.8 Hz), 4.77 (1H, m), 7.06 (2H, d, J = 8.8 Hz), 7.41 (1H, d, J = 8.4 Hz), 7.59 (1H, d, J = 8.4 Hz), 7.64 (1H, s), 8.29 (2H, d, J = 8.8 Hz), 8.63 (1H, t, J = 5.8 Hz), 13.13 (1H, s).509507 1-141 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.38 (6H, s), 1.66 (2H, m), 1.85 (2H, m), 2.07 (2H, m), 4.36 (2H, d, J = 5.8 Hz), 4.81 (1H, m), 6.98 (2H, d, J = 8.8 Hz), 7.40 (1H, d, J = 7.9 Hz), 7.57 (1H, d, J = 7.9 Hz), 7.62 (1H, s), 8.28 (2H, d, J = 8.8 Hz), 8.63 (1H, t, J = 5.8 Hz), 13.14 (1H, s).521519 1-142 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.38 (6H, s), 2.83 (2H, td, J = 11.3, 5.5 Hz), 4.33 (2H, t, J = 5.8 Hz), 4.37 (2H, d, J = 6.0 Hz), 7.12 (2H, d, J = 8.8 Hz), 7.43 (1H, s), 7.58 (1H, s), 7.64 (1H, s), 8.32 (2H, d, J = 8.8 Hz), 8.64 (1H, d, J = 6.0 Hz), 13.18 (1H, s).563561 1-143 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.38 (3H, s), 1.39 (6H, s), 4.18 (2H, s), 4.32 (2H, d, J = 5.8 Hz), 4.37 (2H d, J = 5.8 Hz), 4.51 (2H, d, J = 5.8 Hz), 7.15 (2H, d, J = 8.8 Hz), 7.43 (1H, d, J = 7.7 Hz), 7.60 (1H, d, J = 7.7 Hz), 7.64 (1H, s), 8.33 (2H, d, J = 8.8 Hz), 8.64 (1H, t, J = 5.8 Hz), 13.17 (1H, s).551549 1-144 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.38 (6H, s), 3.32 (3H, s), 3.69 (2H, t, J = 4.4 Hz), 4.21 (2H, t, J = 4.4 Hz), 4.37 (2H, d, J = 5.8 Hz), 7.11 (2H, d, J = 8.8 Hz), 7.42 (1H, d, J = 8.1 Hz), 7.60 (1H, d, J = 8.1 Hz), 7.64 (1H, s), 8.31 (2H, d, J = 8.8 Hz), 8.63 (1H, d, J = 5.8 Hz), 13.15 (1H, s).525523

TABLE 1-191-145 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.91 (3H, t, J = 7.6 Hz), 1.38 (6H, s), 1.80 (2H, q, J = 7.6 Hz), 4.21 (2H, s), 4.35 (2H, d, J = 6.0 Hz), 4.37 (2H, d, J = 5.8 Hz), 4.46 (2H, d, J = 6.0 Hz), 7.15 (2H, d, J = 8.8 Hz), 7.42 (1H, d, J = 8.4 Hz), 7.59 (1H, d, J = 8.4 Hz), 7.64 (1H, s), 8.32 (2H, d, J = 8.8 Hz), 8.64 (1H, t, J = 5.8 Hz), 13.17 (1H, s).565563 1-146 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.38 (6H, s), 1.84 (3H, t, J = 2.2 Hz), 4.37 (2H, d, J = 5.8 Hz), 4.87 (2H, d, J = 2.2 Hz), 7.12 (2H, d, J = 8.8 Hz), 7.43 (1H, d, J = 8.4 Hz), 7.60 (1H, d, J = 8.4 Hz), 7.65 (1H, s), 8.32 (2H, d, J = 8.8 Hz), 8.64 (1H, t, J = 5.8 Hz), 13.17 (1H, s).519517 1-147 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.38 (6H, s), 2.53 (3H, s), 4.37 (2H, d, J = 6.0 Hz), 7.42 (1H, dd, J = 8.1, 2.1 Hz), 7.59 (1H, d, J = 8.1 Hz), 7.63 (1H, d, J = 2.1 Hz), 7.84 (1H, d, J = 8.1 Hz), 8.30 (1H, d, J = 8.1 Hz), 8.34 (1H, s), 8.64 (1H, t, J = 6.0 Hz), 13.53 (1H, br s).533531 1-148 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.54 (3H, s), 2.54 (3H, s), 3.36 (3H, s), 4.33-4.44 (2H, m), 7.47 (1H, dd, J = 8.3, 2.0 Hz), 7.62 (1H, d, J = 8.3 Hz), 7.66 (1H, d, J = 2.0 Hz), 7.86 (1H, d, J = 8.3 Hz), 8.31 (1H, d, J = 8.3 Hz), 8.35 (1H, s), 9.04 (1H, t, J = 6.4 Hz), 13.53 (1H, br s).549547 1-149 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.22-1.36 (4H, m), 2.53 (3H, s), 4.34 (2H, d, J = 6.0 Hz), 7.40 (1H, dd, J = 8.4, 2.1 Hz), 7.57 (1H, d, J = 8.4 Hz), 7.61 (1H, d, J = 2.1 Hz), 7.82 (1H, d, J = 8.4 Hz), 8.30 (1H, d, J = 8.4 Hz), 8.34 (1H, s), 8.47 (1H, t, J = 6.0 Hz), 13.53 (1H, br s).531529 1-150 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.38 (6H, s), 2.31 (3H, s), 2.32 (3H, s), 4.37 (2H, d, J = 6.0 Hz), 7.33 (1H, d, J = 8.2 Hz), 7.43 (1H, d, J = 8.2 Hz), 7.58-7.67 (2H, m), 8.07 (1H, d, J = 8.2 Hz), 8.12 (1H, s), 8.65 (1H, t, J = 6.0 Hz), 13.23 (1H, br s).479477 1-151 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.21 (3H, t, J = 7.5 Hz), 1.54 (3H s), 2.71 (2H, q, J = 7.5 Hz), 3.36 (3H, s), 4.32-4.45 (2H m), 7.33 (1H, t, J = 9.3 Hz), 7.46 (1H, dd, J = 8.4, 2.0 Hz), 7.61 (1H, d, J = 8.4 Hz), 7.66 (1H, s), 8.20-8.26 (1H, m), 8.29 (1H, dd, J = 7.6, 2.3 Hz), 9.04 (1H, t, J = 6.3 Hz), 13.35 (1H, br s).513511 1-152 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.19-1.27 (5H, m), 1.32-1.37 (2H, m), 2.71 (2H, q, J = 7.5 Hz), 4.35 (2H, d, J = 6.0 Hz), 7.33 (1H, t, J = 9.2 Hz), 7.45 (1H, dd, J = 8.4, 2.0 Hz), 7.61 (1H, d, J = 8.4 Hz), 7.65 (1H, s), 8.21-8.26 (1H, m), 8.30 (1H, dd, J = 7.5, 2.0 Hz), 8.48 (1H, t, J = 6.0 Hz), 13.34 (1H, br s).495493

TABLE 1-201-153 [Image Omitted]

1H-NMR (DMSO-D6) : 1.39 (6H, s), 4.37 (2H, d, J = 6.0 Hz), 7.45 (1H, dd, J = 8.3, 2.1 Hz), 7.59-7.66 (3H, m), 8.32-8.38 (1H, m), 8.45 (1H, dd, J = 7.4, 2.1 Hz), 8.65 (1H, t, J = 6.0 Hz), 13.48 (0H, br s).503501 1-154 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.99 (3H, t, J = 7.4 Hz), 1.22-1.27 (2H, m), 1.31-1.37 (2H, m), 1.71-1.81 (2H, m), 4.04 (2H, t, J = 6.5 Hz), 4.34 (2H, d, J = 6.0 Hz), 7.09 (2H, d, J = 9.0 Hz), 7.43 (1H, dd, J = 8.4, 2.0 Hz), 7.59 (1H, d, J = 8.4 Hz), 7.64 (1H, s), 8.31 (2H, d, J = 9.0 Hz), 8.47 (1H, t, J = 6.0 Hz), 13.14 (1H, br s).507505 1-155 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.90 (3H, t, J = 7.3 Hz), 1.27 (3H, d, J = 6.0 Hz), 1.37 (6H, s), 1.43 (2H, m), 1.52-1.60 (1H, m), 1.67 (1H, m), 4.37 (2H, d, J = 5.8 Hz), 4.62 (1H, m), 7.07 (2H, d, J = 9.1 Hz), 7.42 (1H, d, J = 8.1 Hz), 7.59 (1H, d, J = 8.1 Hz), 7.64 (1H, s), 8.29 (2H, d, J = 9.1 Hz), 8.63 (1H, t, J = 5.8 Hz), 13.13 (1H, s).537535 1-156 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.90 (3H, t, J = 7.3 Hz), 1.27 (3H, d, J = 6.0 Hz), 1.36 (6H, s), 1.43 (2H, m), 1.51-1.60 (1H, m), 1.65 (1H, m), 4.37 (2H, d, J = 5.8 Hz), 4.62 (1H, m), 7.06 (2H, d, J = 8.8 Hz), 7.41 (1H, d, J = 8.1 Hz), 7.59 (1H, d, J = 8.1 Hz), 7.64 (1H, s), 8.29 (2H, d, J = 8.8 Hz), 8.63 (1H, t, J = 5.8 Hz), 13.13 (1H, s).537535 1-157 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.77-0.83 (2H, m), 1.02-1.08 (2H, m), 1.22-1.27 (2H, m), 1.31-1.36 (2H, m), 2.07-2.15 (1H, m), 4.34 (2H, d, J = 5.8 Hz), 7.33 (1H, t, J = 9.3 Hz), 7.44 (1H, d, J = 8.4 Hz), 7.60 (1H, d, J = 8.4 Hz), 7.65 (1H, s), 7.96 (1H, d, J = 7.9 Hz), 8.15-8.20 (1H, m), 8.47 (1H, t, J = 5.8 Hz), 13.31 (1H, br s).507505 1-158 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.99 (3H, t, J = 7.5 Hz), 1.21 (6H, s), 1.76 (2H, m), 3.03 (2H, s), 4.04 (2H, t, J = 6.5 Hz), 4.40 (2H, s), 7.09 (2H, d, J = 8.9 Hz), 7.47 (1H, d, J = 8.3 Hz), 7.65 (1H, d, J = 8.3 Hz), 7.67 (1H, s), 8.31 (2H, d, J = 8.9 Hz), 13.14 (1H, s).453451 1-159 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.72-0.77 (2H, m), 0.97-1.04 (2H, m), 1.38 (6H, s), 1.99-2.07 (1H, m), 4.37 (2H, d, J = 6.0 Hz), 7.34-7.45 (3H, m), 7.59 (1H, d, J = 8.4 Hz), 7.65 (1H, s), 8.03 (1H, s), 8.10 (1H, d, J = 7.7 Hz), 8.64 (1H, t, J = 6.0 Hz), 13.32 (1H, br s).491489 1-160 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.72-0.78 (2H, m), 0.98-1.04 (2H, m), 1.21-1.27 (2H, m), 1.31- 1.36 (2H, m), 2.00-2.07 (1H, m), 4.34 (2H, d, J = 5.8 Hz), 7.35-7.46 (3H, m), 7.60 (1H, d, J = 8.1 Hz), 7.66 (1H, s), 8.03 (1H, s), 8.10 (1H, d, J = 7.4 Hz), 8.47 (1H, t, J = 5.8 Hz), 13.30 (1H, br s).489487

TABLE 1-211-161 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.32-1.38 (9H, m), 2.55 (3H, s), 4.10 (2H, q, J = 7.2 Hz), 4.35 (2H, d, J = 5.8 Hz), 6.86-6.92 (2H, m), 7.39 (1H, d, J = 8.6 Hz), 7.56 (1H, d, J = 8.6 Hz), 7.62 (1H, s), 7.81 (1H, br s), 8.61 (1H, t, J = 5.8 Hz), 13.08 (1H, br s).509507 1-162 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.35 (2H, q, J = 5.1 Hz), 0.57-0.60 (2H, m), 1.22-1.30 (1H, m), 1.54 (3H, s), 3.36 (3H, s), 3.93 (2H, d, J = 7.0 Hz), 4.37 (2H, dd, J = 5.9, 2.9 Hz), 7.07 (2H, d, J = 8.8 Hz), 7.42 (1H, d, J = 8.4 Hz), 7.58 (1H, d, J = 8.4 Hz), 7.65 (1H, s), 8.29 (2H, d, J = 8.8 Hz), 9.02 (1H, t, J = 5.9 Hz), 13.14 (1H, br s).537535 1-163 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.33-0.37 (2H, m), 0.57-0.61 (2H, m), 1.22-1.35 (5H, m), 3.92 (2H, d, J = 7.0 Hz), 4.33 (2H, d, J = 5.8 Hz), 7.05 (2H, d, J = 8.8 Hz), 7.39 (1H, dd, J = 8.3, 1.9 Hz), 7.55 (1H, d, J = 8.3 Hz), 7.61 (1H, d, J = 1.9 Hz), 8.28 (2H, d, J = 8.8 Hz), 8.46 (1H, t, J = 5.8 Hz), 13.15 (1H, br s).519517 1-164 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 105 (2H, m), 1.10 (2H, m), 1.38 (6H, s), 4.20 (2H, s), 4.34 (2H, d, J = 5.6 Hz), 7.02 (2H, d, J = 7.9 Hz), 7.32 (1H, d, J = 7.5 Hz), 7.49 (1H, d, J = 7.5 Hz), 7.56 (1H, s), 8.26 (2H, d, J = 7.9 Hz), 8.61 (1H, t, J = 5.6 Hz), 13.18 (1H, s).589587 1-165 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.77-0.82 (2H, m), 1.01-1.07 (2H, m), 1.54 (3H, s), 2.07-2.15 (1H, m), 3.35 (3H, s), 4.31-1.44 (2H, m), 7.32 (1H, t, J = 9.3 Hz), 7.44 (1H, d, J = 8.4 Hz), 7.60 (1H, d, J = 8.4 Hz), 7.67 (1H, s), 7.95 (1H, d, J = 7.2 Hz), 8.14-8.19 (1H, m), 9.02 (1H, t, J = 6.3 Hz), 13.31 (1H br s).525523 1-166 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.33-0.38 (2H, m), 0.56-0.62 (2H, m), 1.19-1.31 (7H, m), 2.59 (2H, q, J = 12.1 Hz), 3.93 (2H, d, J = 7.1 Hz), 4.33 (2H, d, J = 6.0 Hz), 7.07 (2H, d, J = 9.0 Hz), 7.42 (1H, d, J = 8.4 Hz), 7.58 (1H, d, J = 8.4 Hz), 7.63 (1H, s), 8.29 (2H, d, J = 9.0 Hz), 8.35 (1H, t, J = 6.0 Hz), 13.14 (1H, br s).535533 1-167 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.11-0.16 (2H, m), 0.41-0.48 (2H, m), 0.79-0.90 (1H, m), 1.38 (6H, s), 1.65 (2H, q, J = 6.5 Hz), 4.14 (2H, t, J = 6.5 Hz), 4.37 (2H, d, J = 6.0 Hz), 7.09 (2H d, J = 9.0 Hz), 7.41 (1H, dd, J = 8.4, 2.0 Hz), 7.59 (1H, d, J = 8.4 Hz), 7.64 (1H, d, J = 2.0 Hz), 8.30 (2H, d, J = 9.0 Hz), 8.64 (1H, t, J = 6.0 Hz), 13.15 (1H, br s).535533 1-168 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.32-0.38 (2H, m), 0.56-0.62 (2H, m), 1.01 (3H, s), 1.12 (3H, s), 1.19-1.30 (1H, m), 1.78-1.85 (2H, m), 1.90- 1.97 (2H, m), 2.95-3.05 (1H, m), 3.93 (2H, d, J = 7.0 Hz), 4.30 (2H, d, J = 6.0 Hz), 7.08 (2H, d, J = 8.6 Hz), 7.43 (1H, d, J = 8.4 Hz), 7.58 (1H, d, J = 8.4 Hz), 7.62 (1H, s), 8.25- 8.32 (3H, m), 13.13 (1H, br s).493491

TABLE 1-221-169 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.32-0.38 (2H, m), 0.55-0.62 (2H, m), 1.20-1.29 (1H, m), 1.74 (3H, s), 3.93 (2H, d, J = 6.7 Hz), 4.43 (2H, d, J = 6.0 Hz), 7.07 (2H, d, J = 8.4 Hz), 7.41 (1H, d, J = 8.4 Hz), 7.60 (1H, d, J = 8.4 Hz), 7.65 (1H, s), 8.29 (2H, d, J = 8.4 Hz), 9.10 (1H, t, J = 6.0 Hz), 13.14 (1H, br s).575573 1-170 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.33-0.38 (2H, m), 0.56-0.62 (2H, m), 1.20-1.31 (1H, m), 1.57- 1.90 (6H, m), 1.97-2.09 (2H, m), 2.29-2.39 (1H, m), 3.93 (2H, d, J = 7.1 Hz), 4.31-4.33 (2H, br m), 7.08 (2H, d, J = 8.8 Hz), 7.44 (1H, d, J = 8.4 Hz), 7.58 (1H, d, J = 8.4 Hz), 7.64 (1H, s), 8.29 (2H, d, J = 8.8 Hz), 8.50 (1H, t, J = 6.0 Hz), 13.14 (1H, br s).529527 1-171 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.33-0.38 (2H, m), 0.56-0.62 (2H, m), 0.79 (6H, t, J = 7.5 Hz), 1.20-1.54 (5H, m), 1.98-2.07 (1H, m), 3.94 (2H, d, J = 7.1 Hz), 4.34 (2H, d, J = 6.0 Hz), 7.08 (2H, d, J = 9.0 Hz), 7.46 (1H, d, J = 8.2 Hz), 7.60 (1H, d, J = 8.2 Hz), 7.66 (1H, s), 8.29 (2H, d, J = 9.0 Hz), 8.46 (1H, t, J = 5.7 Hz), 13.14 (1H, br s).481479 1-172 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.99-1.33 (5H, m), 1.38 (6H, s), 1.62-1.86 (6H, m), 3.89 (2H, d, J = 6.2 Hz), 4.36 (2H, d, J = 6.0 Hz), 7.06 (2H, d, J = 9.0 Hz), 7.40 (1H, dd, J = 8.4, 2.0 Hz), 7.57 (1H, d, J = 8.4 Hz), 7.62 (1H, d, J = 2.0 Hz), 8.29 (2H, d, J = 9.0 Hz), 8.64 (1H, t, J = 6.0 Hz), 13.15 (1H, br s).563561 1-173 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.33-0.37 (2H, m), 0.56-0.62 (2H, m), 1.11 (6H, s), 1.17 (6H, s), 1.20-1.29 (2H, m), 3.93 (2H, d, J = 7.1 Hz), 4.29 (2H, d, J = 6.0 Hz), 7.07 (2H, d, J = 8.8 Hz), 7.43 (1H, dd, J = 8.4, 2.0 Hz), 7.57 (1H, d, J = 8.4 Hz), 7.62 (1H, d, J = 2.0 Hz), 8.29 (2H, d, J = 8.8 Hz), 8.37 (1H, t, J = 6.0 Hz), 13.14 (1H, br s).507505 1-174 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 2.51 (3H, s), 7.49- 7.46 (3H, m), 7.63-7.60 (2H, m), 7.66 (1H, d, J = 7.9 Hz), 7.77 (2H, d, J = 8.6 Hz), 7.96 (1H, d, J = 7.9 Hz), 8.22 (1H, s), 8.53 (2H, d, J = 8.6 Hz), 13.46 (1H, s).389387 1-175 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.36 (2H, td, J = 5.2, 3.9 Hz), 0.56-0.60 (2H, m), 1.23-1.30 (1H, m), 1.38 (6H, s), 2.21 (3H, s), 3.91 (2H, d, J = 6.7 Hz), 4.34 (2H, d, J = 5.8 Hz), 6.97 (1H, d, J = 8.6 Hz), 7.29 (1H, dd, J = 8.3, 1.9 Hz), 7.47 (1H, d, J = 8.4 Hz), 7.53 (1H, d, J = 1.9 Hz), 8.08-8.14 (2H, m), 8.61 (1H, t, J = 5.8 Hz).535533

TABLE 1-231-176 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.30 (2H, td, J = 5.3, 4.0 Hz), 0.56 (2H, ddd, J = 9.2, 5.3, 3.1 Hz), 1.22-1.27 (1H, m), 1.38 (6H, s), 2.29 (6H, s), 3.69 (2H, d, J = 7.0 Hz), 4.36 (2H, d, J = 5.8 Hz), 7.41 (1H, dd, J = 8.4, 1.9 Hz), 7.58 (1H, d, J = 8.4 Hz), 7.61 (1H, d, J = 1.9 Hz), 8.02 (2H, s), 8.64 (1H, t, J = 5.8 Hz), 13.18 (1H, br s).549547 1-177 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.88 (6H, d, J = 6.4 Hz), 1.54 (3H, s), 1.83- 1.98 (1H, m), 2.55 (2H, d, J = 7.3 Hz), 3.36 (3H, s), 4.31-4.45 (2H, m), 7.35 (2H, d, J = 8.3 Hz), 7.45 (1H, dd, J = 8.1, 2.0 Hz), 7.60 (1H, d, J = 8.1 Hz), 7.67 (1H, br s), 8.26 (2H, d, J = 8.3 Hz), 9.03 (1H, t, J = 6.2 Hz), 13.26 (1H, br s).523521 1-178 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.99 (6H, d, J = 6.4 Hz), 1.20-1.39 (4H, m), 1.96-2.13 (1H, m), 3.84 (2H, d, J = 6.4 Hz), 4.33 (2H, d, J = 6.0 Hz), 7.05 (2H, d, J = 9.1 Hz), 7.37 (1H, dd, J = 8.3, 2.0 Hz), 7.54 (1H, d, J = 8.3 Hz), 7.60 (1H, d, J = 2.0 Hz), 8.28 (2H, d, J = 9.1 Hz), 8.46 (1H, t, J = 5.8 Hz), 13.15 (1H, br s).521519 1-179 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.94 (3H, s), 0.95 (3H, s), 1.27-1.36 (2H, m), 1.38 (6H, s), 1.41-1.49 (2H, m), 1.56- 1.66 (2H, m), 1.80-1.89 (2H, m), 4.36 (2H, d, J = 5.7 Hz), 4.45-4.53 (1H, m), 7.07 (2H, d, J = 9.0 Hz), 7.40 (1H, dd, J = 8.2, 2.0 Hz), 7.58 (1H, d, J = 8.2 Hz), 7.63 (1H, d, J = 2.0 Hz), 8.28 (2H, d, J = 9.0 Hz), 8.63 (1H, t, J = 6.1 Hz), 13.13 (1H, br s).577575 1-180 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.91 (3H, t, J = 7.3 Hz), 1.27-1.37 (2H, m), 1.38 (6H, s), 1.55-1.64 (2H, m), 2.67 (2H, t, J = 7.7 Hz), 4.37 (2H, d, J = 6.0 Hz), 7.38 (2H, d, J = 8.2 Hz), 7.42 (1H, d, J = 8.4 Hz), 7.59 (1H, d, J = 8.4 Hz), 7.64 (1H, s), 8.25 (2H, d, J = 8.2 Hz), 8.64 (1H, t, J = 6.0 Hz), 13.27 (1H, br s).507505 1-181 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.90 (3H, t, J = 7.7 Hz), 1.26-1.37 (2H, m), 1.54 (3H, s), 1.55-1.64 (2H, m), 2.67 (2H, t, J = 7.7 Hz), 3.36 (3H, s), 4.32-4.44 (2H, m), 7.38 (2H, d, J = 7.5 Hz), 7.44 (1H, d, J = 8.4 Hz), 7.59 (1H, d, J = 8.4 Hz), 7.66 (1H, s), 8.25 (2H, d, J = 7.5 Hz), 9.03 (1H, t, J = 6.2 Hz), 13.27 (1H, br s).523521 1-182 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.99 (6H, t, J = 6.0 Hz), 1.37 (6H, s), 2.00-2.10 (1H, m), 4.11 (2H, d, J = 6.5 Hz), 4.34 (2H, d, J = 6.0 Hz), 6.88 (1H, d, J = 8.7 Hz), 7.30 (1H, dd, J = 8.1, 2.1 Hz), 7.48 (1H, d, J = 8.1 Hz), 7.57 (1H, d, J = 2.1 Hz), 8.47 (1H, dd, J = 8.7, 2.4 Hz), 8.60 (1H, t, J = 6.0 Hz), 9.02 (1H, d, J = 2.4 Hz).524522 1-183 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.24 (6H, s), 2.32 (3H, d, J = 1.3 Hz), 2.59 (2H, q, J = 12.1 Hz), 4.34 (2H, d, J = 6.2 Hz), 7.32 (1H, t, J = 9.2 Hz), 7.44 (1H, dd, J = 8.4, 2.0 Hz), 7.59 (1H, d, J = 8.4 Hz), 7.63 (1H, d, J = 2.0 Hz), 8.19-8.25 (1H, m), 8.28 (1H, d, J = 7.7 Hz), 8.35 (1H, t, J = 5.8 Hz), 13.33 (1H, br s).497495

TABLE 1-241-184 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.29-0.39 (2H, m), 0.47-0.55 (2H, m), 1.08-1.17 (1H, m), 1.32 (3H, d, J = 6.3 Hz), 1.38 (6H, s), 4.10-4.18 (1H, m), 4.36 (2H, d, J = 6.0 Hz), 7.05 (2H, d, J = 8.8 Hz), 7.41 (1H, d, J = 8.0 Hz), 7.58 (1H, d, J = 8.0 Hz), 7.63 (1H, s), 8.27 (2H, d, J = 8.8 Hz), 8.63 (1H, t, J = 6.0 Hz), 13.13 (1H, br s).535533 1-185 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.29-0.39 (2H, m), 0.47-0.55 (2H, m), 1.08-1.17 (1H, m), 1.32 (3H, d, J = 6.3 Hz), 1.38 (6H, s), 4.10-4.18 (1H, m), 4.36 (2H, d, J = 6.0 Hz), 7.05 (2H, d, J = 8.8 Hz), 7.41 (1H, d, J = 8.0 Hz), 7.58 (1H, d, J = 8.0 Hz), 7.63 (1H, s), 8.27 (2H, d, J = 8.8 Hz), 8.63 (1H, t, J = 6.0 Hz), 13.13 (1H, br s).535533 1-186 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.37 (6H, s), 2.28 (3H, d, J = 1.2 Hz), 4.33 (2H, d, J = 6.0 Hz), 7.15 (1H, t, J = 9.1 Hz), 7.22 (1H, dd, J = 8.4, 2.3 Hz), 7.41 (1H, d, J = 8.4 Hz), 7.49 (1H, d, J = 2.3 Hz), 8.10-8.16 (1H, m), 8.19 (1H, dd, J = 8.1, 1.6 Hz), 8.59 (1H, t, J = 6.0 Hz).505503 1-187 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.02 (9H, s), 3.75 (2H, s), 4.57 (2H, d, J = 5.7 Hz), 5.42 (1H, t, J = 5.7 Hz), 7.10 (2H, d, J = 8.8 Hz), 7.53 (1H, d, J = 8.4 Hz), 7.59 (1H, d, J = 8.4 Hz), 7.71 (1H, s), 8.31 (2H, d, J = 8.8 Hz), 13.13 (1H, br s).400398 1-188 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.45 (3H, d, J = 6.8 Hz), 1.49 (3H, s), 2.32 (3H, s), 3.33 (3H, s), 5.02-5.10 (1H, m), 7.32 (1H, t, J = 9.3 Hz), 7.60 (2H, s), 7.76 (1H, s), 8.18-8.24 (1H, m), 8.27 (1H, d, J = 6.8 Hz), 8.81 (1H, d, J = 8.4 Hz), 13.32 (1H, s).5135111 1-189 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.46 (3H, d, J = 6.8 Hz), 1.54 (3H, s), 2.31 (3H, s), 3.37 (3H, s), 5.02-5.11 (1H, m), 7.32 (1H, t, J = 9.2 Hz), 7.54 (1H, d, J = 8.4 Hz), 7.61 (1H, d, J = 8.4 Hz), 7.72 (1H, s), 8.19-8.25 (1H, m), 8.28 (1H, d, J = 7.3 Hz), 8.83 (1H, d, J = 8.2 Hz), 13.33 (1H, br s).5135111 1-190 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.33-0.37 (2H, m), 0.56-0.62 (2H, m), 1.21-1.30 (1H, m), 2.85 (3H, br s), 2.90 (3H, br s), 3.94 (2H, d, J = 7.1 Hz), 5.13 (2H, s), 7.09 (2H, d, J = 8.6 Hz), 7.56-7.67 (2H, br m), 7.78 (1H, br s), 8.30 (2H, d, J = 8.6 Hz), 13.15 (1H, br s).455453 1-191 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.30-1.40 (9H, m), 1.44-1.55 (3H, m), 1.64-1.74 (2H, m), 1.80- 1.88 (2H, m), 2.63-2.70 (1H, m), 4.33 (2H, d, J = 6.0 Hz), 7.25 (1H, dd, J = 8.6, 2.1 Hz), 7.40-7.46 (3H, m), 7.52 (1H, d, J = 2.1 Hz), 8.25 (2H, d, J = 8.1 Hz), 8.59 (1H, t, J = 6.0 Hz).557555

TABLE 1-251-192 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.35 (2H, dt, J = 7.9, 2.8 Hz), 0.57-0.61 (2H, m), 1.21-1.30 (1H, m), 3.94 (2H, d, J = 7.0 Hz), 7.09 (2H, d, J = 8.8 Hz), 7.77 (1H, d. J = 8.4 Hz), 8.09 (1H, dd, J = 8.4, 1.6 Hz), 8.30 (2H, d, J = 9.0 Hz), 8.33 (1H, s), 13.31 (1H, s).398396 1-193 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.35 (2H, td, J = 5.2, 4.1 Hz), 0.56-0.62 (2H, m), 1.21-1.29 (1H, m), 3.94 (2H, d, J = 7.0 Hz), 7.08 (2H, d, J = 8.8 Hz), 7.58 (1H, s), 7.74 (1H, d, J = 8.4 Hz), 8.06 (1H, d, J = 8.4 Hz), 8.17 (1H, s), 8.27 (1H, d, J = 2.1 Hz), 8.31 (2H, d, J = 8.8 Hz), 13.14 (1H, s).397395 1-194 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.35 (2H, q, J = 4.8 Hz), 0.57-0.61 (2H, m), 1.22-1.29 (1H, m), 2.81 (3H, d, J = 4.4 Hz), 3.94 (2H, d, J = 7.0 Hz), 7.09 (2H, d, J = 8.8 Hz), 7.75 (1H, d, J = 8.4 Hz), 8.02 (1H, d, J = 8.1 Hz), 8.24 (1H, s), 8.31 (2H, d, J = 8.8 Hz), 8.63-8.67 (1H, m), 13.19 (1H, s).411409 1-195 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.33-0.37 (2H, m), 0.57-0.61 (2H, m), 1.20-1.30 (1H, m), 2.95 (3H, s), 3.00 (3H, s), 3.94 (2H, d, J = 7.0 Hz), 7.09 (2H, d, J = 8.8 Hz), 7.63 (1H, d, J = 9.1 Hz), 7.70 (1H, d, J = 8.1 Hz), 7.83 (1H, d, J = 1.9 Hz), 8.30 (2H, d, J = 9.1 Hz), 13.16 (1H, s).425423 1-196 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.02 (9H, s), 1.51 (6H, s), 3.05 (3H, s), 3.75 (2H, s), 4.65 (2H, s), 7.10 (2H, d, J = 8.8 Hz), 7.41 (1H, d, J = 8.6 Hz), 7.61-7.66 (2H, m), 8.31 (2H, d, J = 8.8 Hz), 13.15 (1H, br s).551549 1-197 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.02 (9H, s), 3.34 (3H, s), 3.75 (2H, s), 4.49 (2H, s), 7.10 (2H, d, J = 8.8 Hz), 7.53 (1H, dd, J = 8.2, 2.0 Hz), 7.62 (1H, d, J = 8.2 Hz), 7.72 (1H, d, J = 2.0 Hz), 8.31 (2H, d, J = 8.8 Hz), 13.14 (1H, br s).414412 1-198 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.02 (9H, s), 2.79 (2H, t, J = 6.6 Hz), 3.65 (2H, td, J = 6.6, 5.1 Hz), 3.75 (2H, s), 4.69 (1H, t, J = 5.1 Hz), 7.10 (2H, d, J = 8.8 Hz), 7.47 (1H, s), 7.54 (1H, d, J = 8.1 Hz), 7.63 (1H, s), 8.31 (2H, d, J = 8.8 Hz), 13.10 (1H, br s).414412 1-199 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.02 (9H, s), 2.89 (2H, t, J = 6.5 Hz), 3.25 (3H, s), 3.58 (2H, t, J = 6.5 Hz), 3.75 (2H, s), 7.10 (2H, d, J = 8.8 Hz), 7.47 (1H, d, J = 8.1 Hz), 7.55 (1H, d, J = 8.1 Hz), 7.65 (1H, d, J = 1.9 Hz), 8.31 (2H, d, J = 8.8 Hz), 13.10 (1H, s).428426

TABLE 1-261-200 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.02 (9H, s), 1.45 (3H, d, J = 7.1 Hz), 1.49 (3H, s), 3.33 (3H, s), 3.75 (2H, s), 5.02-5.11 (1H, m), 7.09 (2H, d, J = 8.8 Hz), 7.58 (2H, s), 7.76 (1H, s), 8.29 (2H, d, J = 8.8 Hz), 8.82 (1H, d, J = 8.2 Hz), 13.14 (1H, br s).5675652 1-201 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.02 (9H, s), 1.47 (3H, d, J = 7.1 Hz), 1.54 (3H, s), 3.37 (3H, s), 3.75 (2H, s), 5.02-5.11 (1H, m), 7.09 (2H, d, J = 8.2 Hz), 7.51 (1H, d, J = 8.4 Hz), 7.59 (1H, d, J = 8.4 Hz), 7.72 (1H, s), 8.31 (2H, d, J = 8.2 Hz), 8.83 (1H, d, J = 8.2 Hz), 13.15 (1H, br s).5675652 1-202 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.48 (6H, s), 4.29 (2H, d, J = 6.0 Hz), 7.08 (1H, t, J = 7.6 Hz), 7.21 (2H, t, J = 7.6 Hz), 7.30 (2H, d, J = 8.4 Hz), 7.33-7.41 (2H, m), 7.52-7.60 (3H, m), 7.68 (1H, t, J = 7.2 Hz), 8.03 (1H, t, J = 6.0 Hz), 8.34 (2H, d, J = 8.1 Hz), 13.33 (1H, br s).459457 1-203 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.00 (9H, t, J = 16.5 Hz), 1.29 (6H, s), 2.80 (2H, t, J = 7.0 Hz), 3.30 (4H, s), 3.34 (3H, q, J = 6.7 Hz), 3.73 (2H, s), 7.07 (2H, d, J = 8.8 Hz), 7.37 (1H, dd, J = 8.4, 2.0 Hz), 7.51 (1H, d, J = 8.4 Hz), 7.57 (1H, d, J = 2.1 Hz), 8.00 (1H, t, J = 5.6 Hz), 8.29 (2H, d, J = 8.8 Hz), 13.10 (1H, br s).551549 1-204 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.89 (6H, d, J = 6.7 Hz), 1.02 (9H, s), 1.81-1.91 (1H, m), 3.25 (2H, d, J = 6.5 Hz), 3.75 (2H, s), 4.53 (2H, s), 7.10 (2H, d, J = 8.8 Hz), 7.54 (1H, d, J = 8.4 Hz), 7.62 (1H, d, J = 8.1 Hz), 7.73 (1H, s), 8.31 (2H, d, J = 8.8 Hz), 13.12 (1H, s).456454 1-205 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.91 (3H, t, J = 7.3 Hz), 1.02 (9H, s), 1.28-1.37 (2H, m), 1.55-1.62 (2H, m), 2.65 (2H, t, J = 7.7 Hz), 3.75 (2H, s), 7.10 (2H, d, J = 9.1 Hz), 7.43 (1H, d, J = 8.1 Hz), 7.53 (1H, d, J = 8.1 Hz), 7.61 (1H, d, J = 2.1 Hz), 8.31 (2H, d, J = 9.1 Hz), 13.09 (1H, s).426424 1-206 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.88 (6H, d, J = 6.6 Hz), 1.51 (6H, s), 1.84-1.95 (1H, m), 2.55 (2H, d, J = 7.3 Hz), 3.05 (3H, s), 4.65 (2H, s), 7.35 (2H, d, J = 8.4 Hz), 7.41 (1H, d, J = 8.2 Hz), 7.62-7.66 (2H, m), 8.26 (2H, d, J = 8.4 Hz), 13.26 (1H, br s).521519 1-207 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.01 (9H, s), 3.75 (2H, s), 4.67 (2H, d, J = 5.5 Hz), 5.55 (1H, t, J = 5.5 Hz), 7.10 (2H, d, J = 8.8 Hz), 7.73 (1H, d, J = 7.9 Hz), 7.78 (1H, s), 7.90 (1H, d, J = 7.9 Hz), 8.29 (2H, d, J = 8.8 Hz), 13.21 (1H, br s).434432

TABLE 1-271-208 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.01 (9H, s), 1.39 (6H, s), 3.74 (2H, s), 4.44 (2H, d, J = 6.0 Hz), 7.08 (2H, d, J = 8.6 Hz), 7.57 (1H, d, J = 8.1 Hz), 7.68 (1H, s), 7.88 (1H, d, J = 8.1 Hz), 8.28 (2H, d, J = 8.6 Hz), 8.69 (1H, t, J = 6.0 Hz), 13.23 (1H, br s).571569 1-209 [Image Omitted]

1H-NMR (CDCl3) Î´: 1.07 (9H, s), 1.12 (3H, t, J = 5.8 Hz), 1.48 (9H, br s), 3.27 (2H, br s), 3.70 (2H, s), 4.48 (2H, s), 7.02 (2H, d, J = 8.6 Hz), 7.41 (1H, br s), 7.50 (1H, d, J = 8.1 Hz), 7.89 (1H, s), 8.50 (2H, d, J = 8.4 Hz).527525 1-210 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.02 (9H, s), 1.23 (3H, t, J = 7.2 Hz), 2.99 (2H, dt, J = 19.7, 7.2 Hz), 3.76 (2H, s), 4.23 (2H, t, J = 6.0 Hz), 7.13 (2H, d, J = 9.1 Hz), 7.74 (2H, s), 7.97 (1H, s), 8.33 (2H, d, J = 9.1 Hz), 9.05 (2H, s).427425 1-211 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.00-1.32 (5H, m), 1.51 (6H, s), 1.61-1.85 (6H, m), 3.05 (3H, s), 3.89 (2H, d, J = 6.2 Hz), 4.65 (2H, s), 7.08 (2H, d, J = 9.0 Hz), 7.40 (1H, d, J = 8.2 Hz), 7.61-7.65 (2H, m), 8.30 (2H, d, J = 9.0 Hz), 13.14 (1H, br s).577575 1-212 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.02 (9H, s), 3.24 (2H, q, J = 9.5 Hz), 3.75 (2H, s), 3.86 (2H, s), 7.10 (2H, d, J = 8.6 Hz), 7.54-7.63 (2H, m), 7.74 (1H, s), 8.32 (2H, d, J = 8.6 Hz), 13.11 (1H, s).481479 1-213 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.30-1.41 (3H, m), 1.44-1.56 (9H, m), 1.65-1.73 (2H, m), 1.81- 1.88 (2H, m), 2.66-2.73 (1H, m), 3.05 (3H, s), 4.65 (2H, s), 7.42 (1H, d, J = 8.6 Hz), 7.54 (2H, d, J = 8.4 Hz), 7.62-7.66 (2H, m), 8.30 (2H, d, J = 8.4 Hz), 13.36 (1H, br s).571569 1-214 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.02 (9H, s), 1.09 (6H, s), 2.72 (2H, s), 3.75 (2H, s), 4.40 (1H, s), 7.10 (2H, d, J = 8.8 Hz), 7.44 (1H, d, J = 8.8 Hz), 7.53 (1H, d, J = 8.1 Hz), 7.61 (1H, s), 8.31 (2H, d, J = 8.8 Hz), 13.10 (1H, br s).442440 1-215 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.02 (9H, s), 1.10 (6H, 3), 2.81 (2H, s), 3.17 (3H, s), 3.75 (2H, s), 7.10 (2H, d, J = 8.8 Hz), 7.41 (1H, dd, J = 8.5, 1.7 Hz), 7.53 (1H, d, J = 8.5 Hz), 7.58 (1H, d, J = 1.7 Hz), 8.31 (2H, d, J = 8.8 Hz), 13.10 (1H, s).456454

TABLE 1-281-216 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.00 (9H, s), 1.06 (3H, d, J = 6.3 Hz), 2.68 (2H, d, J = 6.0 Hz), 3.73 (2H, s), 3.82-3.88 (1H, m), 4.60 (1H, d, J = 4.9 Hz), 7.09 (2H, d, J = 9.1 Hz), 7.42 (1H, d, J = 9.3 Hz), 7.51 (1H, d, J = 8.1 Hz), 7.59 (1H, s), 8.30 (2H, d, J = 9.1 Hz), 13.08 (1H, s).428426 1-217 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.02 (9H, s), 1.20 (9H, s), 2.36 (2H, t, J = 7.6 Hz), 2.86 (2H, t, J = 7.6 Hz), 3.75 (2H, s), 7.10 (2H, d, J = 8.8 Hz), 7.40-7.46 (2H, m), 7.53 (1H, d, J = 8.2 Hz), 7.61 (1H, s), 8.31 (2H, d, J = 8.8 Hz), 13.11 (1H, br s).497495 1-218 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.02 (9H, s), 2.66 (2H, t, J = 7.6 Hz), 2.81 (3H, s), 2.87 (2H, t, J = 7.6 Hz), 2.95 (3H, s), 3.75 (2H, s), 7.10 (2H, d, J = 8.8 Hz), 7.46-7.56 (2H, m), 7.66 (1H, d, J = 2.0 Hz), 8.31 (2H, d, J = 9.0 Hz), 13.11 (1H, br s).469467 1-219 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.89 (6H, d, J = 6.5 Hz), 1.30-1.40 (3H, m), 1.46-1.55 (3H, m), 1.65-1.73 (2H, m), 1.80-1.90 (3H, m), 2.67- 2.72 (1H, m), 3.25 (2H, d, J = 6.5 Hz), 4.53 (2H, s), 7.54 (2H, d, J = 8.4 Hz), 7.57 (1H, d, J = 1.6 Hz), 7.64 (1H, d, J = 8.1 Hz), 7.73 (1H, s), 8.31 (2H, d, J = 8.4 Hz), 13.33 (1H, br s).476474 1-220 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.51 (6H, s), 3.06 (3H, s), 4.65 (2H, s), 7.42 (1H, d, J = 8.4 Hz), 7.56 (2H, t, J = 7.7 Hz), 7.62-7.69 (3H, m), 8.34 (2H, d, J = 7.7 Hz), 13.34 (1H, br s).465463 1-221 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.24 (9H, s), 3.02 (3H, s), 4.62 (2H, s), 7.40 (1H, dd, J = 8.4, 1.8 Hz), 7.56 (2H, t, J = 7.6 Hz), 7.61-7.69 (3H, m), 8.34 (2H, d, J = 7.6 Hz), 13.34 (1H, br s).465463 1-222 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.01 (1.8H, d, J = 6.7 Hz), 1.04 (4.2H, d, J = 6.7 Hz), 2.84 (0.9H, s), 2.84-2.88 (0.3H, m), 2.90-2.97 (0.7H, m), 3.01 (2.1H, s), 4.57 (1.4H, s), 4.71 (0.6H, s), 7.42 (1H, dd, J = 8.3, 1.9 Hz), 7.57 (2H, t, J = 7.6 Hz), 7.60-7.69 (3H, m), 8.34 (2H, d, J = 7.6 Hz), 13.34 (1H, br s).397395 1-223 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.02 (9H, s), 1.37 (9H, s), 2.79 (3H, s), 3.75 (2H, s), 4.50 (2H, s), 7.11 (2H, d, J = 8.9 Hz), 7.55 (1H, dd, J = 8.1, 1.6 Hz), 7.68 (1H, d, J = 8.1 Hz), 7.72 (1H, s), 8.31 (2H, d, J = 8.9 Hz), 13.17 (1H, br s).533531

TABLE 1-291-224 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.97-1.05 (3H, m), 2.34-2.43 (2H, m), 2.85 (0.9H, s), 2.96 (2.1H, s), 4.57 (1.4H, s), 4.65 (0.6H, s), 7.44 (1H, d, J = 8.4 Hz), 7.54-7.69 (5H, m), 8.34 (2H, d, J = 8.4 Hz), 13.35 (1H, br s).383381 1-225 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.84-0.93 (3H, m), 1.50-1.60 (2H, m), 2.31-2.38 (2H, m), 2.84 (0.9H, s), 2.96 (2.1H, s), 4.57 (1.4H, s), 4.66 (0.6H, s), 7.41-7.46 (1H, m), 7.54-7.69 (5H, m), 8.34 (2H, d, J = 7.6 Hz), 13.34 (1H, br s).397395 1-226 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.87-0.93 (6H, m), 1.99-2.10 (1H, m), 2.22-2.28 (2H, m), 2.84 (0.9H, s), 2.97 (2.1H, s), 4.58 (1.4H, s), 4.66 (0.6H, s), 7.40-7.46 (1H, m), 7.56 (2H, t, J = 7.6 Hz), 7.60-7.70 (3H, m), 8.34 (2H, d, J = 7.6 Hz), 13.34 (1H, br s).411409 1-227 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.02 (9H, s), 1.43 (6H, s), 2.88 (2H, t, J = 7.5 Hz), 3.03 (3H, s), 3.55 (2H, t, J = 7.5 Hz), 3.74 (2H, s), 7.09 (2H, d, J = 9.0 Hz), 7.45 (1H, dd, J = 8.0, 2.0 Hz), 7.55 (1H, d, J = 8.0 Hz), 7.64 (1H, d, J = 2.0 Hz), 8.30 (2H, d, J = 9.0 Hz), 13.11 (1H, br s).565563 1-228 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.46-1.86 (8H, m), 2.83 (0.9H, s), 2.96-3.10 (1H, m), 3.01 (2.1H, S), 4.57 (1.4H, s), 4.72 (0.6H, s), 7.42 (1H, d, J = 8.3 Hz), 7.56 (2H, t, J = 7.6 Hz), 7.60-7.69 (3H, m), 8.34 (2H, d, J = 7.6 Hz), 13.33 (1H, br s).423421 1-229 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.10-1.43 (5H, m), 1.58-1.77 (5H, m), 2.56-2.68 (1H, m), 2.81 (0.9H, s), 3.01 (2.1H, s), 4.56 (1.4H, s), 4.70 (0.6H, s), 7.38-7.43 (1H, m), 7.56 (2H, t, J = 7.6 Hz), 7.59-7.69 (3H, m), 8.34 (2H, d, J = 7.6 Hz), 13.34 (1H, br s).437435 1-230 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 2.87-2.97 (3H, m), 4.54 (0.8H, s), 4.75 (1.2H, s), 7.39-7.51 (5.4H, m), 7.54-7.61 (2.6H, m), 7.62-7.70 (2.4H, m), 7.74-7.83 (0.6H, m), 8.35 (2H, d, J = 7.6 Hz), 13.35 (1H, br s).431429 1-231 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.79 (3H, t, J = 7.4 Hz), 1.19 (6H, s), 1.64 (2H, q, J = 7.4 Hz), 3.02 (3H, s), 4.61 (2H, s), 7.43 (1H, d, J = 8.3 Hz), 7.56 (2H. t, J = 7.6 Hz), 7.61-7.69 (3H, m), 8.34 (2H, d, J = 7.6 Hz), 13.33 (1H, br s).425423

TABLE 1-301-232 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.88 (6H, d, J = 6.7 Hz), 1.35 (9H, s), 1.84-1.95 (1H, m), 2.55 (2H, d, J = 7.4 Hz), 2.90 (3H, s), 3.77 (2H, s), 7.35 (2H, d, J = 8.1 Hz), 7.41 (1H, d, J = 8.3 Hz), 7.57 (1H, d, J = 8.3 Hz), 7.62 (1H, s), 8.26 (2H, d, J = 8.1 Hz), 13.24 (1H, br s).467465 1-233 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.75-0.84 (6H, m), 1.33-1.46 (2H, m), 1.47-1.60 (2H, m), 2.62- 2.71 (1H, m), 2.88 (0.9H, s), 3.03 (2.1H, s), 4.61 (1.4H, s), 4.72 (0.6H, s), 7.41-7.47 (1H, m), 7.56 (2H, t J = 7.6 Hz), 7.61-7.69 (3H, m), 8.33 (2H, d, J= 7.6 Hz), 13.35 (1H, br s).425423 1-234 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.37 (6H, s), 2.78 (0.6H, br s), 3.14 (3H, s), 3.23 (2.4H, br s), 4.59 (1.6H, s), 5.09 (0.4H, br s), 7.45 (1H, d, J = 7.9 Hz), 7.56 (2H, t, J = 7.9 Hz). 7.61-7.69 (3H, m), 8.33 (2H, d, J = 7.9 Hz), 13.33 (1H, br s).427425 1-235 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.02 (9H, s), 1.12 (3H, t, J = 6.8 Hz), 1.50 (6H, s), 3.42 (2H, br s), 3.75 (2H, s), 4.66 (2H, s), 7.09 (2H, d, J = 8.8 Hz), 7.40 (1H, d, J = 8.3 Hz), 7.61 (1H, d, J = 8.3 Hz), 7.62 (1H, s), 8.30 (2H, d, J = 8.8 Hz), 13.14 (1H, s).565563 1-236 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.21 (6H, s), 3.04 (2H, s), 4.41 (2H, s), 7.48 (1H, d, J = 8.6 Hz), 7.57 (2H, t, J = 7.6 Hz), 7.64-7.71 (3H, m), 8.35 (2H, d, J = 7.6 Hz), 13.34 (1H, br s).395393 1-237 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.48 (6H, s), 2.45 (3H, br s), 4.55 (2H, br s), 7.18 (1H, t, J = 7.2 Hz), 7.22-7.26 (2H, m), 7.28-7.34 (2H, m), 7.41 (1H, br s), 7.53-7.68 (5H, m), 8.35 (2H, d, J = 8.1 Hz), 13.34 (1H, br s).473471 1-238 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.56-1.67 (4H, m), 2.08-2.17 (2H, m), 2.37-2.46 (2H, m), 3.02 (3H, s), 4.65 (2H, s), 7.39 (1H, d, J = 8.3 Hz), 7.55 (2H, t, J = 8.0 Hz), 7.60-7.68 (3H, m), 8.34 (2H, d, J = 8.0 Hz), 13.34 (1H, br s).491489 1-239 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.23 (6H, s), 3.02 (3H, s), 3.20 (3H, s), 3.43 (2H, s), 4.63 (2H, s), 7.42 (1H, d, J = 8.3 Hz), 7.54-7.70 (5H, m), 8.34 (2H, d, J = 7.6 Hz), 13.36 (1H, br s).441439

TABLE 1-311-240 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.07 (6H, s), 1.83 (2H t, J = 6.9 Hz), 3.21 (2H, t, J = 6.9 Hz), 4.44 (2H, s), 7.42 (1H, d, J = 8.3 Hz), 7.57 (2H, t, J = 7.6 Hz), 7.62-7.69 (3H, m), 8.34 (2H, d, J = 7.6 Hz), 13.34 (1H, br s).409407 1-241 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.15 (6H, s), 1.64- 1.69 (2H, m), 1.74-1.81 (2H, m), 3.26 (2H, t, J = 6.0 Hz), 4.53 (2H, s), 7.42 (1H, d, J = 8.1 Hz), 7.56 (2H, t, J = 7.4 Hz), 7.60-7.69 (3H, m), 8.35 (2H, d, J = 7.4 Hz), 13.33 (1H, br s).423421 1-242 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.61 (3H, s), 2.82 (0.6H. br s), 3.23 (2.4H, s), 3.37 (3H, s), 4.64 (1.6H, s), 4.87-5.12 (0.4H, m), 7.46 (1H, d, J = 8.3 Hz), 7.56 (2H, t, J = 7.6 Hz), 7.63-7.71 (3H, m), 8.34 (2H, d, J = 7.6 Hz), 13.34 (1H, br s).481479 1-243 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.61 (3H, s), 2.82 (0.6H, br s), 3.23 (2.4H, s), 3.37 (3H, s), 4.64 (1.6H, s), 4.87-5.12 (0.4H, m), 7.46 (1H, d, J = 8.3 Hz), 7.56 (2H, t, J = 7.6 Hz), 7.63-7.71 (3H, m), 8.34 (2H, d, J = 7.6 Hz), 13.34 (1H, br s).481479 1-244 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.18-1.31 (3H, m), 1.46-1.59 (3H, m), 1.63-1.72 (2H, m), 2.51- 2.58 (2H, m), 3.15 (3H, s), 4.71 (2H, s), 7.43 (1H, dd, J = 8.1, 1.8 Hz), 7.56 (2H, t, J = 7.5 Hz), 7.63-7.69 (3H, m), 8.34 (2H, d, J = 7.5 Hz), 13.36 (1H, br s).505503 1-245 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.81 (3H, t, J = 7.4 Hz), 1.15-1.25 (8H, m), 1.54-1.60 (2H, m), 3.02 (3H, s), 4.61 (2H, s), 7.42 (1H, d, J = 8.6 Hz), 7.56 (2H, t, J = 7.4 Hz), 7.60-7.69 (3H, m), 8.34 (2H, d, J = 7.4 Hz), 13.34 (1H, br s).439437 1-246 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 2.88 (0.9H, s), 3.04- 3.22 (6.1H, m), 3.63-3.77 (1H, m), 4.62 (1.4H, s), 4.80 (0.6H, s), 7.42-7.48 (1H, m), 7.52- 7.58 (2H, m), 7.60-7.69 (3H, m), 8.34 (2H, d, J = 7.9 Hz), 13.35 (1H, br s).471469 1-247 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.14-1.36 (5H, m), 1.38-1.50 (3H, m), 1.89-1.97 (2H, m), 3.37 (2H, s), 4.36 (2H, d, J = 6.0 Hz), 4.75 (1H, br s), 7.49 (1H, d, J = 8.3 Hz), 7.53-7.59 (3H, m), 7.64-7.70 (2H, m), 8.09 (1H, t, J = 6.0 Hz), 8.34 (2H, d, J = 7.4 Hz), 13.32 (1H, br s).453451

TABLE 1-321-248 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.26-1.37 (3H, m), 1.42-1.50 (1H, m), 1.59-1.71 (6H, m), 3.02 (2H, s), 4.40 (2H, s), 7.47 (1H, dd, J = 8.1, 2.1 Hz), 7.56 (2H, t, J = 7.9 Hz), 7.63-7.69 (3H, m), 8.34 (2H, d, J = 7.9 Hz), 13.33 (1H, br s).435433 1-249 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.21 (6H, s), 2.94 (2H, s), 3.11 (3H, s), 4.63 (2H, s), 7.06-7.10 (2H, m), 7.11-7.16 (1H, m), 7.16-7.22 (2H, m), 7.39 (1H, dd, J = 7.9, 1.8 Hz), 7.55 (2H, t, J = 7.4 Hz), 7.59-7.67 (3H, m), 8.34 (2H, d, J = 7.4 Hz), 13.35 (1H, br s).453451 1-250 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.71 (6H, t, J = 7.4 Hz), 1.51 (4H, q, J = 7.4 Hz), 3.47 (2H, d, J = 4.0 Hz), 4.34 (2H, d, J = 5.7 Hz), 4.62 (1H, br s), 7.48 (1H, d, J = 7.7 Hz), 7.53-7.61 (3H, m), 7.64-7.71 (2H, m), 8.11 (1H, t, J = 5.7 Hz), 8.34 (2H, d, J = 7.3 Hz), 13.34 (1H, br s).441439 1-251 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.87 (6H, t, J = 7.4 Hz), 1.52-1.63 (4H, m), 3.01 (2H, s), 4.40 (2H, s), 7.49 (1H, d, J = 8.3 Hz), 7.57 (2H, t, J = 7.9 Hz), 7.64-7.72 (3H, m), 8.34 (2H, d, J = 7.9 Hz), 13.35 (1H, br s).423421 1-252 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.02 (0.6H, br s), 1.17 (2.4H, t, J = 6.9 Hz), 1.62 (3H, s), 2.81 (0.6H, s), 3.25 (2.4H, s), 3.54-3.67 (2H, m), 4.63 (1.6H, s), 4.90-5.17 (0.4H, m), 7.45 (1H, dd, J = 8.2, 1.8 Hz), 7.56 (2H, t, J = 7.6 Hz), 7.63-7.69 (3H, m), 8.34 (2H, d, J = 7.6 Hz), 13.34 (1H, br s).495493 1-253 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.02 (0.6H, br s), 1.17 (2.4H, t, J = 6.9 Hz), 1.62 (3H, s), 2.81 (0.6H, s), 3.25 (2.4H, s), 3.54-3.67 (2H, m), 4.63 (1.6H, s), 4.90-5.17 (0.4H, m), 7.45 (1H, dd, J = 8.2, 1.8 Hz), 7.56 (2H, t, J = 7.6 Hz), 7.63-7.69 (3H, m), 8.34 (2H, d, J = 7.6 Hz), 13.34 (1H, br s).495493 1-254 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.51-1.55 (6H, m), 1.86-1.95 (2H, m), 3.46 (2H, s), 4.35 (2H, d, J = 6.0 Hz), 4.99 (1H, br s), 7.47 (1H, dd, J = 8.4, 2.0 Hz), 7.54-7.60 (3H, m), 7.64-7.69 (2H, m), 8.12 (1H, t, J = 6.0 Hz), 8.34 (2H, d, J = 7.7 Hz), 13.34 (1H, br s).439437 1-255 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.53-1.68 (4H, m), 1.77-1.89 (4H, m), 3.17 (2H, s), 4.42 (2H, s), 7.47 (1H, dd, J = 8.3, 2.1 Hz), 7.56 (2H, t, J = 7.6 Hz), 7.64-7.69 (3H, m), 8.35 (2H, d, J = 7.6 Hz).421419

TABLE 1-331-256 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.69 (3H, d, J = 6.2 Hz), 0.85-0.98 (2H, m), 1.06-1.28 (3H, m), 1.42-1.50 (2H, m), 2.05-2.12 (2H, m), 3.28 (2H, s), 4.36 (2H, d, J = 6.0 Hz), 4.73 (1H, br s), 7.49 (1H, d, J = 8.1 Hz), 7.53-7.59 (3H, m), 7.63-7.69 (2H, m), 8.10 (1H, t, J = 6.0 Hz), 8.34 (2H, d, J = 7.9 Hz), 13.34 (1H, br s).4674653 1-257 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.89 (3H, d, J = 5.5 Hz), 1.33-1.46 (3H, m), 1.51-1.62 (4H, m), 1.87-1.94 (2H, m), 2.94 (2H, s), 4.39 (2H, s), 7.47 (1H, dd, J = 8.3, 2.3 Hz), 7.56 (2H, t, J = 7.9 Hz), 7.63-7.69 (3H, m), 8.34 (2H, d, J = 7.9 Hz), 13.33 (1H, br s).4494473 1-258 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.34 (6H, s), 4.99 (2H, s), 6.97 (1H, d, J = 7.6 Hz), 7.05 (1H, t, J = 7.6 Hz), 7.20 (1H, t, J = 7.6 Hz), 7.39 (1H, d, J = 7.6 Hz), 7.48 (1H, dd, J = 8.3, 1.8 Hz), 7.55 (2H, t, J = 7.6 Hz), 7.59-7.68 (2H, m), 7.75 (1H, d, J = 1.8 Hz), 8.29 (2H, d, J = 7.6 Hz), 13.32 (1H, br s).457455 1-259 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.77 (6H, t, J = 7.3 Hz), 1.03-1.19 (4H, m), 1.36-1.50 (4H, m), 3.46 (2H, s), 4.33 (2H, d, J = 6.0 Hz), 4.62 (1H, br s), 7.47 (1H, d, J = 8.4 Hz), 7.53-7.60 (3H, m), 7.61-7.69 (2H, m), 8.12 (1H, t, J = 6.0 Hz), 8.34 (2H, d, J = 7.6 Hz), 13.35 (1H, br s).469467 1-260 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.84 (6H, t, J = 7.2 Hz), 1.14-1.29 (2H, m), 1.30-1.58 (6H, m), 3.02 (2H, s), 4.39 (2H, s), 7.48 (1H, dd, J = 8.3, 2.1Hz), 7.57 (2H, t, J = 7.9 Hz), 7.64- 7.70 (3H, m), 8.34 (2H, d, J = 7.9 Hz), 13.36 (1H, br s).451449 1-261 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.96-1.06 (3.9H, m), 1.10 (2.1 H, t, J = 7.2 Hz), 2.31 (0.6H, q, J = 7.2 Hz), 2.42 (1.4H, q, J = 7.2 Hz), 3.29-3.35 (2H, m), 4.56 (1.4H, s), 4.63 (0.6H, s), 7.45 (1H, d, J = 8.3 Hz), 7.54-7.69 (5H, m), 8.34 (2H, d, J = 7.9 Hz), 13.33 (1H, br s).397395 1-262 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.28 (3H, s), 2.75 (2H, d, J = 15.7 Hz), 3.36 (2H, d, J = 15.7 Hz), 4.37 (2H, d, J = 6.0 Hz), 7.06-7.11 (2H, m), 7.14-7.18 (2H, m), 7.44 (1H, dd, J = 8.3, 2.1 Hz), 7.52-7.62 (4H, m), 7.66 (1H, t, J = 7.4 Hz), 8.34 (2H, d, J = 7.4 Hz), 8.41 (1H, t, J = 6.0 Hz), 13.32 (1H, br s).471469 1-263 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.13 (3H, t, J = 6.9 Hz), 1.50 (6H, s), 3.42 (2H, br s), 4.66 (2H, s), 7.41 (1H, dd, J = 8.3, 1.8 Hz), 7.56 (2H, t, J = 7.9 Hz), 7.61-7.69 (3H, m), 8.34 (2H, d, J = 7.9 Hz), 13.33 (1H, br s).479477

TABLE 1-341-264 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.31 (3H, s), 2.91 (2H, d, J = 16.0 Hz), 3.04 (3H, br s), 3.51 (2H, d, J = 16.0 Hz), 4.66 (2H, br s), 7.11-7.15 (2H, m), 7.17-7.21 (2H, m), 7.45 (1H, dd, J = 8.3, 2.1 Hz), 7.54 (2H, t, J = 7.9 Hz), 7.61- 7.67 (3H, m), 8.33 (2H, d, J = 7.9 Hz), 13.33 (1H, br s).485483 1-265 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.34-1.42 (2H, m), 1.40 (9H, s), 1.53-1.63 (2H, m), 1.96 (2H, t, J = 6.8 Hz), 2.86-3.00 (2H, m), 3.25 (2H, t, J = 6.8 Hz), 3.78-3.87 (2H, m), 4.46 (2H, s), 7.42 (1H, dd, J = 8.3, 2.1 Hz), 7.54-7.59 (2H, m), 7.60-7.69 (3H, m), 8.34 (2H, d, J = 7.7 Hz), 13.34 (1H, br s).550548 1-266 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.83 (9H, s), 0.90- 0.99 (1H, m), 1.41-1.67 (6H, m), 1.96-2.03 (2H, m), 2.92 (2H, s), 4.38 (2H, s), 7.47 (1H, dd, J = 8.3, 1.8 Hz), 7.56 (2H, t, J = 7.6 Hz), 7.63-7.69 (3H, m), 8.34 (2H, d, J = 7.6 Hz), 13.34 (1H, br s).4914894 1-267 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.48-1.65 (4H, m), 1.67-1.77 (2H, m), 1.82-1.91 (2H, m), 3.03 (2H, s), 3.21 (3H, s), 3.23-3.29 (1H, m), 4.40 (2H, s), 7.48 (1H, dd, J = 8.1, 1.8 Hz), 7.56 (2H, t, J = 7.6 Hz), 7.63-7.70 (3H, m), 8.34 (2H. d, J = 7.6 Hz), 13.34 (1H, br s).4654635

TABLE 2-1MSMSExampleStructureNMR(M + H)(M âˆ’ H)Note2-1 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 2.24 (6H, s), 7.21 (2H, d, J = 7.6 Hz), 7.37 (1H, t, J = 7.6 Hz), 7.91 (2H, d, J = 8.4 Hz), 8.53 (2H, d, J = 8.4 Hz), 13.35 (1H, br s).346344 2-2 [Image Omitted]

1H-NMR (CDCl3) Î´: 2.34 (6H, s), 7.17 (2H, d, J = 7.7 Hz), 7.33 (1H, t, J = 7.7 Hz), 7.49 (2H, t, J = 7.7 Hz), 7.60 (1H, t, J = 7.7 Hz), 8.52 (2H, dd, J = 7.7, 1.2 Hz), 11.87 (1H, s).278276 2-3 [Image Omitted]

1H-NMR (CDCl3) Î´: 2.32 (6H, s), 2.72 (3H, s), 7.17 (2H, d, J = 7.7 Hz), 7.34 (1H, t, J = 7.7 Hz), 7.53-7.56 (2H, m), 8.12-8.16 (1H, m), 11.29 (1H, s).360358 2-4 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 3.84 (3H, s), 7.25 (2H, d, J = 8.3 Hz), 7.59 (1H, t, J = 8.3 Hz), 7.92 (2H, d, J = 8.3 Hz), 8.51 (2H, d, J = 8.3 Hz), 13.59 (1H, br s).382380 2-5 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 2.22 (6H, s), 5.22 (2H, s), 7.13-7.21 (2H, m), 7.32-7.37 (2H, m), 7.38-7.43 (2H, m), 7.46-7.48 (2H, m), 8.28- 8.32 (2H, m), 12.98 (1H, br s).384382 2-6 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 2.20 (6H, s), 6.86 (2H, dt, J = 9.4, 2.4 Hz), 7.17 (2H, d, J = 7.7 Hz), 7.32 (1H, t, J = 7.6 Hz), 8.19 (2H, dt, J = 9.5, 2.3 Hz), 10.32 (1H, br s), 12.87 (1H, br s).294292 2-7 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.32 (9H, s), 2.22 (6H, s), 7.20 (2H, d, J = 7.7 Hz), 7.35 (1H, t, J = 7.7 Hz), 7.56 (2H, d, J = 8.5 Hz), 8.28 (2H, d, J = 8.5 Hz), 13.08 (1H, br s).334332 2-8 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.23 (6H, d, J = 7.3 Hz), 2.22 (6H, s), 2.90-3.05 (1H, m), 7.20 (2H, d, J = 7.7 Hz), 7.35 (1H, t, J = 7.7 Hz), 7.41 (2H, d, J = 8.3 Hz), 8.27 (2H, d, J = 8.3 Hz), 13.09 (1H, br s).320318 2-9 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 2.24 (6H, s), 7.19 (2H, d, J = 7.7 Hz), 7.34 (1H, t, J = 7.7 Hz), 7.73 (1H, d, J = 8.1 Hz), 7.85 (1H, d, J = 10.7 Hz), 8.24 (1H, t, J = 7.9 Hz), 13.42 (1H, br s).364362

TABLE 2-22-10 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 7.50 (1H, t, J = 8.8 Hz), 7.57 (1H, d, J = 8.4 Hz), 7.67-7.73 (1H, m), 7.94 (2H, d, J = 8.1 Hz), 8.51 (2H, d, J = 8.1 Hz), 13.90 (1H, br s).370368 2-11 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 2.24 (6H, s), 7.20 (2H d, J = 7.7 Hz), 7.35 (1H, t, J = 7.7 Hz), 7.45-7.47 (3H, m), 7.59-7.63 (2H, m), 7.71 (2H, dd, J = 6.9, 2.0 Hz), 8.37 (2H, dd, J = 6.9, 2.0 Hz), 13.20 (1H, br s).378376 2-12 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 2.22 (6H, s), 2.89- 3.00 (4H, m), 7.16-7.29 (7H, m), 7.33 (1H, d, J = 7.7 Hz), 7.38 (2H, d, J = 8.4 Hz), 8.24 (2H, d, J = 8.4 Hz), 13.09 (1H, br s).382380 2-13 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 2.23 (6H, s), 2.39 (3H, s), 7.20 (2H d, J = 7.6 Hz), 7.35 (1H, t, J = 7.6 Hz), 7.40-7.48 (2H, m), 8.11-8.18 (2H, m), 13.14 (1H, br s).292290 2-14 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 2.23 (6H, s), 3.83 (3H, s), 7.18-7.24 (3H, m), 7.36 (1H, t, J = 7.8 Hz), 7.45 (1H, t, J = 7.8 Hz), 7.83-7.86 (1H, m), 7.94 (1H, d, J = 7.8 Hz), 13.17 (1H, br s).308306 2-15 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 2.23 (6H, s), 7.21 (2H, d, J = 7.6 Hz), 7.36 (1H, t, J = 7.6 Hz), 7.47-7.53 (1H, m), 7.57-7.63 (1H, m), 8.00- 8.05 (1H, m), 8.19 (1H, dt, J = 7.9, 1.3 Hz), 13.27 (1H, br s).296294 2-16 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 6.97 (1H, dd, J = 8.1, 0.7 Hz), 7.05 (1H, dd, J = 8.1, 0.7 Hz), 7.39 (1H, t, J = 8.1 Hz), 7.90 (2H, d, J = 8.4 Hz), 8.50 (2H, d, J = 8.4 Hz), 10.74 (1H, br s), 13.38 (1H, br s).368366 2-17 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.20 (3H, t, J = 7.0 Hz), 4.12 (2H, q, J = 7.0 Hz), 7.21 (2H, d, J = 8.3 Hz), 7.55 (1H, t, J = 8.3 Hz), 7.91 (2H, d, J = 8.3 Hz), 8.50 (2H, d, J = 8.3 Hz), 13.53 (1H, br s).396394 2-18 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.31-1.40 (3H, m), 1.45-1.54 (3H, m), 1.65-1.72 (2H, m), 1.81- 1.88 (2H, m), 2.22 (6H, s), 2.67-2.71 (1H, m), 7.20 (2H, d, J = 7.7 Hz), 7.35 (1H, t, J = 7.7 Hz), 7.51 (2H, dd, J = 6.7, 1.9 Hz), 8.29 (2H, dd, J = 6.7, 1.9 Hz), 13.16 (1H, br s).384382

TABLE 2-32-19 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 2.29 (3H, s), 7.38- 7.41 (1H, m), 7.47-7.52 (2H, m), 7.92 (2H d, J = 8.1 Hz), 8.52 (2H, d, J = 8.1 Hz), 13.61 (1H, br s).366364 2-20 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.80 (3H, t, J = 7.3 Hz), 1.55-1.63 (2H, m), 4.02 (2H, t, J = 6.3 Hz), 7.22 (2H, t, J = 8.3 Hz), 7.55 (1H, t, J = 8.3 Hz), 7.90 (2H, d, J = 8.3 Hz), 8.50 (2H, d, J = 8.3 Hz), 13.54 (1H, br s).410408 2-21 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.18 (6H, d, J = 6.0 Hz), 4.65-4.71 (1H, m), 7.20 (1H, d, J = 8.4 Hz), 7.25 (1H, d, J = 8.4 Hz), 7.54 (1H, t, J = 8.4 Hz), 7.91 (2H, d, J = 8.4 Hz), 8.50 (2H, d, J = 8.4 Hz), 13.49 (1H, br s).410408 2-22 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 3.59-3.62 (2H, br m), 4.09 (2H, t, J = 5.0 Hz), 4.81 (1H, br s), 7.24 (2H, t, J = 8.3 Hz), 7.56 (1H, t, J = 8.3 Hz), 7.92 (2H, d, J = 8.4 Hz), 8.52 (2H, d, J = 8.4 Hz), 13.49 (1H, s).412410 2-23 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.71-1.77 (2H, m), 3.39 (2H, t, J = 6.2 Hz), 4.14 (2H, t, J = 6.2 Hz), 4.45 (1H, br s), 7.23 (1H, d, J = 8.3 Hz), 7.24 (1H, d, J = 8.3 Hz), 7.56 (1H, t, J = 8.3 Hz), 7.92 (2H, d, J = 8.4 Hz), 8.52 (2H, d, J = 8.4 Hz), 13.56 (1H, br s).426424 2-24 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 2.23 (6H, s), 7.20 (2H, d, J = 7.7 Hz), 7.35 (1H, t, J = 7.7 Hz), 7.60 (2H, d, J = 8.6 Hz), 8.34 (2H, d, J = 8.6 Hz), 13.21 (1H, br s).296294 2-25 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 2.23 (6H, s), 7.20 (2H, d, J = 7.6 Hz), 7.35 (1H, t, J = 7.6 Hz), 7.60 (2H, d, J = 8.6 Hz), 8.34 (2H, d, J = 8.6 Hz), 13.21 (1H, br s).312310 2-26 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 2.22 (6H, s), 2.40 (3H, s), 7.20 (2H, d, J = 7.5 Hz), 7.32-7.37 (3H, m), 8.23 (2H, d, J = 8.2 Hz), 13.08 (1H, br s).292290 2-27 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 2.22 (6H, s), 3.85 (3H, s), 7.07 (2H, d, J = 9.0 Hz), 7.19 (2H, d, J = 7.6 Hz), 7.34 (1H, t, J = 7.6 Hz), 8.31 (2H, d, J = 9.0 Hz), 12.98 (1H, s).308306

TABLE 2-42-28 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 2.24 (6H, s), 7.21 (2H, d, J = 7.7 Hz), 7.37 (1H, t, J = 7.7 Hz), 7.80 (1H, t, J = 7.9 Hz), 8.03 (1H, d, J = 7.9 Hz), 8.57 (1H, s), 8.62 (1H, d, J = 7.9 Hz), 13.33 (1H, br s).346344 2-29 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 2.22 (6H, s), 5.19 (2H, s), 7.20 (2H, d, J = 7.5 Hz), 7.27-7.42 (5H, m), 7.43-7.49 (3H, m), 7.92-7.96 (2H, m), 13.17 (1H, br s). 384382 2-30 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 2.22 (6H, s), 6.99- 7.03 (1H, m), 7.20 (2H, d, J = 7.7 Hz), 7.30- 7.37 (2H, m), 7.76-7.80 (2H, m), 9.69 (1H, s), 13.12 (1H, br s).294292 2-31 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.76 (3H, t, J = 7.4 Hz), 1.22-1.31 (2H, m), 1.53-1.60 (2H, m), 4.07 (2H, t, J = 6.2 Hz), 7.23 (2H, dd, J = 8.4, 2.2 Hz), 7.56 (1H, t, J = 8.4 Hz), 7.92 (2H, d, J = 8.4 Hz), 8.51 (2H, d, J = 8.4 Hz), 13.56 (1H, br s).424422 2-32 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 5.24 (2H, s), 7.35- 7.24 (7H, m), 7.57 (1H, t, J = 8.3 Hz), 7.93 (2H, d, J = 8.2 Hz), 8.52 (2H, d, J = 8.2 Hz), 13.66 (1H, br s).458456 2-33 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.80 (6H, d, J = 6.7 Hz), 1.82-1.92 (1H, m), 3.83 (2H, d, J = 6.0 Hz), 7.20 (2H, dd, J = 8.3, 3.5 Hz), 7.54 (1H, t, J = 8.3 Hz), 7.90 (2H, d, J = 8.4 Hz), 8.50 (2H, d, J = 8.4 Hz), 13.56 (1H, br s).424422 2-34 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 3.12 (3H, s), 3.53 (2H, t, J = 4.5 Hz), 4.18 (2H, t, J = 4.5 Hz), 7.24 (2H, dd, J = 8.3, 3.8 Hz), 7.55 (1H, t, J = 8.3 Hz), 7.90 (2H, d, J = 8.4 Hz), 8.50 (2H, d, J = 8.4 Hz), 13.54 (1H, br s).426424 2-35 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 3.83 (3H, s), 5.22 (2H, s), 7.16 (2H, d, J = 8.8 Hz), 7.22 (2H, d, J = 8.8 Hz), 7.37-7.32 (1H, m), 7.43-7.38 (2H, m), 7.50-7.45 (2H, m), 7.61-7.53 (1H, m), 8.28 (2H, d, J = 8.8 Hz), 13.17 (1H, s).420418 2-36 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 4.90 (2H, q, J = 8.7 Hz), 7.38 (2H, dd, J = 8.4, 2.4 Hz), 7.64 (1H, t, J = 8.4 Hz), 7.93 (2H, d, J = 8.2 Hz), 8.51 (2H, d, J = 8.2 Hz), 13.68 (1H, br s).450448

TABLE 2-52-37 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.99-1.10 (2H, m), 1.13-1.30 (3H, m), 1.61-1.83 (6H, m), 2.20 (6H, s), 3.86 (2H, d, J = 6.3 Hz), 7.04 (2H, d, J = 9.1 Hz), 7.17 (2H, d, J = 7.7 Hz), 7.32 (1H, t, J = 7.7 Hz), 8.26 (2H, d, J = 9.1 Hz), 12.95 (1H, br s).390388 2-38 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.28 (6H, d, J = 6.0 Hz), 2.20 (6H, s), 4.70-4.76 (1H, m), 7.02 (2H, d, J = 8.8 Hz), 7.18 (2H, d, J = 7.6 Hz), 7.32 (1H, t, J = 7.6 Hz), 8.26 (2H, d, J = 8.8 Hz), 12.94 (1H, br s).336334 2-39 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.88 (6H, d, J = 6.4 Hz), 1.84-1.94 (1H, m), 2.23 (6H, s), 2.54 (2H, d, J = 6.9 Hz), 7.20 (2H, d, J = 7.7 Hz), 7.30-7.38 (3H, m), 8.26 (2H, d, J = 8.5 Hz), 13.09 (1H, br s).334332 2-40 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.35 (3H, t, J = 6.9 Hz), 2.22 (6H, s), 4.13 (2H, q, J = 6.9 Hz), 7.05 (2H, d, J = 8.8 Hz), 7.20 (2H, d, J = 7.5 Hz), 7.34 (1H, t, J = 7.5 Hz), 8.29 (2H, d, J = 8.8 Hz), 12.97 (1H, br s).322320 2-41 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.99 (3H, t, J = 7.4 Hz), 1.71-1.80 (2H, m), 2.22 (6H, s), 4.03 (2H, t, J = 6.6 Hz), 7.06 (2H, d, J = 9.0 Hz), 7.20 (2H, d, J = 7.5 Hz), 7.34 (1H, t, J = 7.5 Hz), 8.29 (2H, d, J = 9.0 Hz), 12.98 (1H, br s).336334 2-42 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.99 (6H, d, J = 6.8 Hz), 2.01-2.08 (1H, m), 2.22 (6H, s), 3.85 (2H, d, J = 6.6 Hz), 7.06 (2H, d, J = 8.8 Hz), 7.20 (2H, d, J = 7.5 Hz), 7.35 (1H, t, J = 7.5 Hz), 8.29 (2H, d, J = 8.8 Hz), 12.97 (1H, br s).350348 2-43 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.58 (3H, d, J = 6.4 Hz), 2.19 (6H, s), 5.66 (1H, q, J = 6.4 Hz), 7.04 (2H, d, J = 9.0 Hz), 7.17 (2H, d, J = 7.7 Hz), 7.23-7.28 (1H, m), 7.30-7.37 (3H, m), 7.41-7.43 (2H, m), 8.20 (2H, d, J = 9.0 Hz), 12.96 (1H, br s).398396 2-44 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.58 (3H, d, J = 6.4 Hz), 2.19 (6H, s), 5.66 (1H, q, J = 6.4 Hz), 7.04 (2H, d, J = 9.0 Hz), 7.18 (2H, d, J = 7.7 Hz), 7.24-7.28 (1H, m), 7.30-7.37 (3H, m), 7.41-7.43 (2H, m), 8.20 (2H, d, J = 9.0 Hz), 12.96 (1H, br s).398396 2-45 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 5.32 (2H, s), 7.34- 7.27 (4H, m), 7.57 (1H, t, J = 8.2 Hz), 7.71 (1H, td, J = 7.7, 1.7 Hz), 7.93 (2H, d, J = 8.2 Hz), 8.55-8.51 (3H, m), 13.74 (1H, s).459457

TABLE 2-62-46 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.23-1.32 (1H, m), 1.35-1.57 (5H, m), 1.68-1.75 (2H, m), 1.92- 1.98 (2H, m), 2.22 (6H, s), 4.47-4.51 (1H, m), 7.06 (2H, d, J = 8.8 Hz), 7.19 (2H, d, J = 7.6 Hz), 7.34 (1H, t, J = 7.6 Hz), 8.27 (2H, d, J = 8.8 Hz), 12.96 (1H, br s).376374 2-47 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.94 (6H, d, J = 4.2 Hz), 1.26-1.35 (2H, m), 1.41-1.48 (2H, m), 1.56-1.65 (2H, m), 1.81-1.87 (2H, m), 2.22 (6H, s), 4.46-4.50 (1H, m), 7.06 (2H, d, J = 8.8 Hz), 7.19 (2H, d, J = 7.5 Hz), 7.34 (1H, t, J = 7.5 Hz), 8.27 (2H, d, J = 8.8 Hz), 12.96 (1H, br s).404402 2-48 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 2.23 (6H, s), 5.31 (2H, s), 7.19-7.23 (2H, m), 7.32-7.43 (4H, m), 7.48-7.53 (2H, m), 7.63 (1H, d, J = 8.4 Hz), 7.97 (1H, dd, J = 8.4, 1.7 Hz), 8.10 (1H, d, J = 1.7 Hz), 13.22 (1H, br s).418416 2-49 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.99 (3H, t, J = 7.5 Hz), 2.55 (2H, q, J = 7.5 Hz), 5.22 (2H, s), 7.07 (1H, t, J = 6.8 Hz), 7.16 (1H, d, J = 6.8 Hz), 7.22 (1H, t, J = 6.8 Hz), 7.27 (1H, d, J = 8.2 Hz), 7.31 (1H, d, J = 6.8 Hz), 7.42 (1H, d, J = 8.2 Hz), 7.60 (1H, t, J = 8.2 Hz), 7.91 (2H, d, J = 8.6 Hz), 8.48 (2H, d, J = 8.6 Hz), 13.63 (1H, s).486484 2-50 [Image Omitted]

1H-NMR (DMSO-D6) Î´:1.30-1.38 (2H, m), 1.50-1.65 (4H, m), 1.74-1.82 (2H, m), 2.22 (6H, s), 2.29-2.36 (1H, m), 3.95 (2H, d, J = 7.1 Hz), 7.06 (2H, d, J = 8.8 Hz), 7.20 (2H, d, J = 7.7 Hz), 7.34 (1H, t, J = 7.7 Hz), 8.28 (2H, d, J = 8.8 Hz), 12.97 (1H, br s). 376374 2-51 [Image Omitted]

1H-NMR (DMSO-D6) Î´:1.81-1.94 (4H, m), 2.05-2.12 (2H, m), 2.22 (6H, s), 2.70-2.78 (1H, m), 4.05 (2H, d, J = 6.6 Hz), 7.06 (2H, d, J = 8.8 Hz), 7.20 (2H, d, J = 7.5 Hz), 7.34 (1H, t, J = 7.5 Hz), 8.29 (2H, d, J = 8.8 Hz), 12.97 (1H, br s).362360 2-52 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.38 (3H, t, J = 6.9 Hz), 2.22 (6H, s), 4.19 (2H, q, J = 6.9 Hz), 7.19 (2H, d, J = 7.6 Hz), 7.34 (1H, t, J = 7.6 Hz), 7.58 (1H, d, J = 8.3 Hz), 7.92 (1H, dd, J = 8.3, 1.7 Hz), 7.96 (1H, d, J = 1.7 Hz), 13.19 (1H, br s).356354 2-53 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 2.18 (3H, s), 5.18 (2H, s), 7.04 (1H, t. J = 7.4 Hz), 7.16-7.09 (2H, m), 7.21 (1H, d, J = 7.9 Hz), 7.28 (1H, d, J = 7.4 Hz), 7.34 (1H, d, J = 8.4 Hz), 7.57- 7.49 (1H, m), 7.88 (2H, d, J = 8.4 Hz), 8.47 (2H, d, J = 8.4 Hz), 13.63 (1H, s).472470 2-54 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.20 (3H, t, J = 7.5 Hz), 2.23 (6H, s), 2.80 (2H, q, J = 7.5 Hz), 7.21 (2H, d, J = 7.6 Hz), 7.36 (1H, t, J = 7.6 Hz), 7.58 (1H, d, J = 8.4 Hz), 8.17 (1H, dd, J = 8.4, 2.0 Hz), 8.29 (1H, d, J = 2.0 Hz), 13.20 (1H, br s).340338

TABLE 2-72-55 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 2.11 (3H, s), 5.17 (2H, s), 7.04 (1H, d, J = 7.7 Hz), 7.17-7.06 (3H, m), 7.25 (1H, d, J = 8.3 Hz), 7.29 (1H, d, J = 8.3 Hz), 7.57 (1H, t, J = 8.3 Hz), 7.91 (2H, d, J = 8.1 Hz), 8.52 (2H, d, J = 8.1 Hz), 13.64 (1H, br s).472470 2-56 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 2.22 (3H, s), 5.16 (2H, s), 7.06 (2H, d, J = 7.9 Hz), 7.20 (2H, d, J = 7.9 Hz), 7.23 (1H, d, J = 7.9 Hz), 7.28 (1H, d, J = 7.9 Hz), 7.55 (1H, t, J = 8.4 Hz), 7.92 (2H, d, J = 8.4 Hz), 8.49 (2H, d, J = 8.4 Hz), 13.62 (1H, br s).472470 2-57 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.34-1.23 (2H, m), 1.62-1.38 (6H, m), 1.72-1.63 (2H, m), 1.86- 1.77 (2H, m), 2.00-1.88 (1H, m), 2.22 (6H, s), 3.86 (2H, d, J = 6.8 Hz), 7.06 (2H, d, J = 8.8 Hz), 7.20 (2H, d, J = 7.6 Hz), 7.34 (1H, t, J = 7.6 Hz), 8.28 (2H, d, J = 8.8 Hz), 12.97 (1H, br s).404402 2-58 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.01 (9H, s), 2.22 (6H, s), 3.74 (2H, s), 7.07 (2H, d, J = 8.8 Hz), 7.20 (2H, d, J = 7.7 Hz), 7.35 (1H, t, J = 7.7 Hz), 8.29 (2H, d, J = 8.8 Hz), 12.97 (1H, br s).364362 2-59 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 2.24 (6H, s), 5.46 (2H, s), 7.02 (1H, d, J = 8.8 Hz), 7.20 (2H, d, J = 7.7 Hz), 7.31-7.41 (4H, m), 7.46-7.49 (2H, m), 8.53 (1H, dd, J = 8.8, 2.0 Hz), 9.10 (1H, d, J = 2.0 Hz), 13.13 (1H, br s).385383 2-60 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 5.30 (2H, s), 7.28 (1H, d, J = 7.9 Hz), 7.30-7.35 (2H, m), 7.59 (1H, t, J = 8.4 Hz), 7.73 (1H, dt, J = 7.9, 1.5 Hz), 7.93 (2H, d, J = 8.4 Hz), 8.48 (1H, dd, J = 4.9, 1.5 Hz), 8.51 (2H, d, J = 8.4 Hz), 8.56 (1H, d, J = 1.5 Hz), 13.67 (1H, br s).459457 2-61 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 5.33 (2H, s), 7.23- 7.32 (4H, m), 7.57 (1H, t, J = 8.4 Hz), 7.94 (2H, d, J = 8.4 Hz), 8.48 (2H, d, J = 5.3 Hz), 8.54 (2H, d, J = 8.4 Hz), 13.72 (1H, br s).459457 2-62 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.00 (9H, s), 3.73 (2H, s), 5.30 (2H, s), 7.08 (2H, t, J = 4.5 Hz), 7.30-7.20 (3H, m), 7.33 (1H, d, J = 7.7 Hz), 7.52 (1H, t, J = 8.1 Hz), 7.69 (1H, td, J = 7.7, 1.7 Hz), 8.28 (2H, d, J = 8.8 Hz), 8.53-8.49 (1H, m), 13.27 (1H, br s).477475 2-63 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.01 (9H, s), 1.46 (3H, d, J = 6.2 Hz), 3.74 (2H, s), 5.60 (1H, q, J = 6.2 Hz), 7.04-7.11 (3H, m), 7.17 (1H, d, J = 7.7 Hz), 7.27 (1H, dd, J = 7.0, 5.1 Hz), 7.37-7.44 (2H, m), 7.72 (1H, t, J = 7.0 Hz), 8.30 (2H, d, J = 8.8 Hz), 8.51 (1H, d, J = 4.2 Hz), 13.32 (1H, br s).491489

TABLE 2-82-64 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.01 (9H, s), 1.47 (3H, d, J = 6.3 Hz), 3.74 (2H, s), 5.60 (1H, q, J = 6.3 Hz), 7.13-7.02 (3H, m), 7.18 (1H, d, J = 7.7 Hz), 7.27 (1H, dd, J = 7.1, 5.2 Hz), 7.48-7.36 (2H, m), 7.72 (1H, t, J = 7.1 Hz), 8.31 (2H, d, J = 8.8 Hz), 8.51 (1H, d, J = 4.2 Hz), 13.32 (1H, br s).491489 2-65 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.01 (9H, s), 1.40 (3H, d, J = 6.2 Hz), 3.74 (2H, s), 5.62 (1H, q, J = 6.2 Hz), 7.05-7.15 (4H, m), 7.21-7.41 (6H, m), 8.31 (2H, d, J = 8.8 Hz), 13.21 (1H, br s).490488 2-66 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.99 (6H, d, J = 6.6 Hz), 2.04 (1H, td, J = 13.3, 6.6 Hz), 3.86 (2H, d, J = 6.6 Hz), 5.31 (2H, s), 7.08 (2H, d, J = 8.8 Hz), 7.31-7.21 (3H, m), 7.34 (1H, d, J = 7.7 Hz), 7.57-7.48 (1H, m), 7.70 (1H, td, J = 7.7, 1.6 Hz), 8.30 (2H, d, J = 8.8 Hz), 8.53 (1H, d, J = 4.9 Hz), 13.29 (1H, br s).463461 2-67 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.00 (6H, d, J = 6.6 Hz), 1.48 (3H, d, J = 6.6 Hz), 2.09-2.02 (1H, m), 3.87 (2H, d, J = 6.6 Hz), 5.61 (1H, q, J = 6.2 Hz), 7.14-7.04 (3H, m), 7.19 (1H, d, J = 7.9 Hz), 7.31-7.25 (1H, m), 7.48-7.37 (2H, m), 7.73 (1H, td, J = 7.9, 1.6 Hz), 8.32 (2H, d, J = 9.0 Hz), 8.53 (1H, d, J = 4.9 Hz), 13.33 (1H, br s).477475 2-68 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.02 (9H, s), 3.74 (2H, s), 5.40 (2H, s), 7.08 (2H, d, J = 8.8 Hz), 7.24 (2H, d, J = 7.7 Hz), 7.44 (1H, t, J = 4.9 Hz), 7.51 (1H, br s), 8.27 (2H, d, J = 8.8 Hz), 8.78 (2H, d, J = 4.9 Hz), 13.19 (1H, br s).478476 2-69 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.88 (3H, t, J = 7.2 Hz), 1.31-1.42 (4H, m), 1.69-1.76 (2H, m), 2.21 (6H, s), 4.05 (2H, t, J = 6.5 Hz), 7.04 (2H, d, J = 8.8 Hz), 7.18 (2H, d, J = 7.4 Hz), 7.33 (1H, t, J = 7.4 Hz), 8.27 (2H, d, J = 8.8 Hz), 12.96 (1H, br s).364362 2-70 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.92 (3H, t, J = 7.3 Hz), 1.38-1.48 (2H, m), 1.67-1.74 (2H, m), 2.21 (6H, s), 4.06 (2H, t, J = 6.5 Hz), 7.05 (2H, d, J = 8.8 Hz), 7.18 (2H, d, J = 7.7 Hz), 7.33 (1H, t, J = 7.7 Hz), 8.27 (2H, d, J = 8.8 Hz), 12.96 (1H, br s).350348 2-71 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.92 (6H, d, J = 6.6 Hz), 1.63 (2H, q, J = 6.6 Hz), 1.72-1.82 (1H, m), 2.21 (6H, s), 4.08 (2H, t, J = 6.6 Hz), 7.05 (2H, d, J = 8.8 Hz), 7.18 (2H, d, J = 7.4 Hz), 7.33 (1H, t, J = 7.4 Hz), 8.27 (2H, d, J = 8.8 Hz), 12.96 (1H, br s).364362 2-72 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.98 (6H, d, J = 6.5 Hz), 2.00-2.07 (1H, m), 2.19 (3H, s), 3.84 (2H, d, J = 6.5 Hz), 5.31 (2H, s), 7.05 (2H, d, J = 8.8 Hz), 7.20-7.26 (2H, m), 7.40 (1H, d, J = 8.1 Hz), 7.50-7.57 (2H, m), 8.23 (2H, d, J = 8.8 Hz), 8.33 (1H, d, J = 3.7 Hz), 13.25 (1H, br s).477475

TABLE 2-92-73 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.90 (6H, t, J = 7.4 Hz), 1.60-1.69 (4H, m), 2.22 (6H, s), 4.36- 4.42 (1H, m), 7.06 (2H, d, J = 8.8 Hz), 7.20 (2H, d, J = 7.5 Hz), 7.35 (1H, t, J = 7.5 Hz), 8.28 (2H, d, J = 8.8 Hz), 12.97 (1H, br s).364362 2-74 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.31-0.35 (2H, m), 0.55-0.59 (2H, m), 1.20-1.27 (1H, m), 2.21 (6H, s), 3.91 (2H, d, J = 7.0 Hz), 7.04 (2H, d, J = 8.8 Hz), 7.18 (2H, d, J = 7.4 Hz), 7.33 (1H, t, J = 7.4 Hz), 8.27 (2H, d, J = 8.8 Hz), 12.95 (1H, br s).348346 2-75 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.94-1.80 (4H, m), 2.11-2.03 (2H, m), 2.76-2.69 (1H, m), 4.05 (2H, d, J = 6.7 Hz), 5.29 (2H, s), 7.07 (2H, d, J = 8.8 Hz), 7.29-7.23 (3H, m), 7.32 (1H, d, J = 7.9 Hz), 7.56-7.48 (1H, m), 7.71-7.66 (1H, m), 8.28 (2H, d, J = 8.8 Hz), 8.52-8.50 (1H, m), 13.28 (1H, br s).475473 2-76 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.40-1.31 (3H, m), 1.54-1.45 (3H, m), 1.73-1.65 (2H, m), 1.88- 1.80 (2H, m), 2.73-2.66 (1H, m), 5.31 (2H, s), 7.31-7.25 (3H, m), 7.33 (1H, d, J = 7.7 Hz), 7.57-7.51 (3H, m), 7.70 (1H, td, J = 7.7, 1.7 Hz), 8.30 (2H, d, J = 8.4 Hz), 8.52 (1H, d, J = 4.6 Hz), 13.53 (1H, br s).497495 2-77 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.30-0.99 (5H, m), 1.83-1.60 (6H, m), 3.87 (2H, d, J = 6.3 Hz), 5.29 (2H, s), 7.06 (2H, d, J = 8.8 Hz), 7.29- 7.23 (3H, m), 7.32 (1H, d, J = 7.7 Hz), 7.55- 7.49 (1H, m), 7.68 (1H, t, J = 7.7 Hz), 8.27 (2H, d, J = 8.8 Hz), 8.51 (1H, d, J = 4.9 Hz), 13.27 (1H, br s).503501 2-78 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.90 (3H, t, J = 7.3 Hz), 1.27 (3H, d, J = 6.0 Hz), 1.47-1.31 (2H, m), 1.60-1.51 (1H, m), 1.72-1.63 (1H, m), 4.65-4.59 (1H, m), 5.31 (2H, s), 7.06 (2H, d, J = 9.0 Hz), 7.31-7.24 (3H, m), 7.34 (1H, d, J = 7.7 Hz), 7.53 (1H, t, J = 7.7 Hz), 7.70 (1H, td, J = 7.7, 1.5 Hz), 8.28 (2H, d, J = 9.0 Hz), 8.53 (1H, d, J = 4.2 Hz), 13.28 (1H, br s).477475 2-79 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.90 (3H, t, J = 7.3 Hz), 1.27 (3H, d, J = 6.0 Hz), 1.47-1.33 (2H, m), 1.61-1.51 (1H, m), 1.72-1.62 (1H, m), 4.65-4.59 (1H, m), 5.31 (2H, s), 7.06 (2H, d, J = 9.0 Hz), 7.31-7.24 (3H, m), 7.34 (1H, d, J = 7.9 Hz), 7.53 (1H, t, J = 7.9 Hz), 7.72-7.68 (1H, m), 8.28 (2H, d, J = 9.0 Hz), 8.53 (1H, d, J = 4.0 Hz), 13.28 (1H, br s).477475 2-80 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 5.31 (2H, s), 7.30- 7.25 (3H, m), 7.33 (1H, d, J = 7.7 Hz), 7.47- 7.43 (3H, m), 7.54 (1H, t, J = 8.5 Hz), 7.62- 7.57 (2H, m), 7.73-7.67 (3H, m), 8.36 (2H, d, J = 8.4 Hz), 8.52 (1H, d, J = 4.0 Hz), 13.56 (1H, br s).491489

TABLE 2-102-81 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.76-1.54 (6H, m), 2.04-1.95 (2H, m), 2.95-2.88 (1H, m), 5.31 (2H, s), 7.34-7.25 (4H, m), 7.58-7.51 (3H, m), 7.70 (1H, td, J = 7.7, 1.8 Hz), 8.29 (2H, d, J = 8.4 Hz), 8.54-8.52 (1H, m), 13.53 (1H, br s).483481 2-82 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.81-0.76 (2H, m), 0.96-0.90 (2H, m), 1.64-1.55 (1H, m), 5.31 (2H, s), 7.33-7.25 (4H, m), 7.58-7.50 (3H, m), 7.70 (1H, td, J = 7.7, 1.7 Hz), 8.28 (2H, d, J = 8.4 Hz), 8.53 (1H, d, J = 4.0 Hz), 13.52 (1H, br s).455453 2-83 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.31-1.40 (3H, m), 1.45-1.54 (3H, m), 1.65-1.72 (2H, m), 1.81- 1.87 (2H, m), 2.67-2.72 (1H, m), 5.44 (2H, s), 7.25-7.30 (2H, m), 7.51-7.56 (4H, m), 8.14 (1H, d, J = 8.2 Hz), 8.29 (2H, d, J = 8.6 Hz), 8.94 (1H, s), 13.52 (1H, br s).565563 2-84 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.94 (6H, d, J = 4.2 Hz), 1.35-1.25 (2H, m), 1.49-1.40 (2H, m), 1.66-1.55 (2H, m), 1.89-1.79 (2H, m), 4.53- 4.44 (1H, m), 5.30 (2H, s), 7.07 (2H, d, J = 8.8 Hz), 7.31-7.21 (3H, m), 7.34 (1H, d, J = 7.7 Hz), 7.56-7.48 (1H, m), 7.70 (1H, t, J = 7.1 Hz), 8.27 (2H, d, J = 8.8 Hz), 8.53 (1H, d, J = 4.6 Hz), 13.28 (1H, s).517515 2-85 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 5.38 (2H, s), 7.28 (1H, d, J = 8.1 Hz), 7.31 (1H, d, J = 8.1 Hz), 7.47-7.40 (5H, m), 7.55 (1H, d, J = 8.4 Hz), 7.61-7.57 (2H, m), 7.72 (2H, d, J = 8.8 Hz), 7.88-7.84 (1H, m), 8.36 (2H, d, J = 8.8 Hz), 8.58 (1H, d, J = 4.4 Hz).491489 2-86 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.51 (3H, d, J = 6.4 Hz), 5.65 (1H, q, J = 6.4 Hz), 7.11 (1H, d, J = 8.6 Hz), 7.22 (1H, d, J = 7.7 Hz), 7.32 (1H, dd, J = 6.9, 5.4 Hz), 7.50-7.41 (5H, m), 7.63- 7.59 (2H, m), 7.81-7.73 (3H, m), 8.40 (2H, d, J = 8.6 Hz), 8.55 (1H, d, J = 4.2 Hz).505503 2-87 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 2.53 (3H, s), 5.40 (2H, s), 7.30 (1H, d, J = 8.2 Hz), 7.33 (1H, d, J = 8.2 Hz), 7.42-7.35 (2H, m), 7.49-7.44 (3H, m), 7.64-7.56 (3H, m), 7.73 (2H, d, J = 8.8 Hz), 7.91-7.88 (1H, m), 8.37 (2H, d, J = 8.8 Hz).505503 2-88 [Image Omitted]

1H-NMR (CDCl3) Î´: 5.50 (0.90H, s), 5.60 (1.10H, s), 7.43-7.30 (5.45H, m), 7.69-7.54 (5.00H, m), 7.90-7.78 (2.00H, m), 8.05 (0.45H, d, J = 7.9 Hz), 8.71-8.60 (2.55H, m), 8.88 (0.55H, d, J = 4.2 Hz), 11.44 (0.45H, s), 14.69 (0.55H, br s).482480 2-89 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.38-0.32 (2H, m), 0.62-0.56 (2H, m), 1.30-1.20 (1H, m), 3.93 (2H, d, J = 6.8 Hz), 5.32 (2H, s), 7.08 (2H, d, J = 7.5 Hz), 7.36-7.24 (4H, m), 7.54 (1H, t, J = 8.4 Hz), 7.71 (1H, t, J = 7.6 Hz), 8.29 (2H, d, J = 7.5 Hz), 8.53 (1H, d, J = 4.6 Hz).461459

TABLE 2-112-90 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.32-0.35 (2H, m), 0.55-0.59 (2H, m), 1.20-1.25 (1H, m), 2.79 (3H, s), 2.91 (3H, s), 3.91 (2H, d, J = 7.0 Hz), 4.98 (2H, s), 7.05 (2H, d, J = 8.8 Hz), 7.16 (1H, d, J = 8.4 Hz), 7.23 (1H, d, J = 8.4 Hz), 7.51 (1H, t, J = 8.4 Hz), 8.26 (2H, d, J = 8.8 Hz), 13.15 (1H, br s).455453 2-91 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.76-1.53 (6H, m), 2.05-1.93 (2H, m), 2.95-2.88 (1H, m), 5.36 (2H, s), 7.28 (1H, dd, J = 7.9, 0.7 Hz), 7.30 (1H, d, J = 7.9 Hz), 7.43-7.36 (2H, m), 7.59- 7.50 (3H, m), 7.81 (1H, td, J = 7.7, 1.8 Hz), 8.29 (2H, d, J = 8.6 Hz), 8.57 (1H, dq, J = 5.0, 0.8 Hz).483481 2-92 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.41-1.30 (3H, m), 1.56-1.45 (3H, m), 1.74-1.64 (2H, m), 1.89- 1.80 (2H, m), 2.74-2.65 (1H, m), 5.37 (2H, s), 7.28 (1H, dd, J = 8.0, 0.6 Hz), 7.31 (1H, d, J = 8.0 Hz), 7.46-7.40 (2H, m), 7.59-7.52 (3H, m), 7.85 (1H, td, J = 7.8, 1.6 Hz), 8.29 (2H, d, J = 8.6 Hz), 8.59-8.58 (1H, m).497495 2-93 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.33-0.37 (2H, m), 0.54-0.59 (2H, m), 1.23-1.30 (1H, m), 4.21 (2H, d, J = 7.3 Hz), 5.23 (2H, s), 6.97 (1H, d, J = 8.8 Hz), 7.23-7.35 (7H, m), 7.55 (1H, t, J = 8.0 Hz), 8.48 (1H, dd, J = 8.8, 2.2 Hz), 9.05 (1H, d, J = 2.2 Hz), 13.41 (1H, br s).461459 2-94 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.31 (9H, s), 5.38 (2H, s), 7.28 (1H, d, J = 8.2 Hz), 7.31 (1H, d, J = 8.2 Hz), 7.47-7.41 (2H, m), 7.52 (2H, d, J = 8.8 Hz), 7.56 (1H, t, J = 8.4 Hz), 7.86 (1H, td, J = 7.7, 1.5 Hz), 8.29 (2H, d, J = 8.8 Hz), 8.59 (1H, dd, J = 5.0, 0.8 Hz).471469 2-95 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 5.38 (2H, s), 7.39- 7.34 (2H, m), 7.46-7.43 (3H, m), 7.54 (1H, d, J = 7.9 Hz), 7.61-7.57 (2H, m), 7.66 (1H, d, J = 8.6 Hz), 7.72 (2H, d, J = 8.4 Hz), 7.82-7.76 (2H, m), 8.34 (2H, d, J = 8.4 Hz), 8.56 (1H, d, J = 4.9 Hz).525523 2-96 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 5.25 (2H, s), 7.36- 7.24 (8H, m), 7.46 (1H, ddd, J = 7.8, 4.9, 1.0 Hz), 7.57 (1H, t, J = 8.3 Hz), 7.72 (1H, dt, J = 7.8, 1.0 Hz), 7.79 (2H, d, J = 8.6 Hz), 7.90 (1H, td, J = 7.8, 1.8 Hz), 8.40 (2H, d, J = 8.6 Hz), 8.65 (1H, dq, J = 4.9, 0.9 Hz).491489 2-97 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 5.40 (2H, s), 7.30 (1H, dd, J = 8.2, 0.7 Hz), 7.33 (1H, d, J = 8.2 Hz), 7.51-7.43 (3H, m), 7.58 (1H, t, J = 8.2 Hz), 7.75 (1H, dt, J = 7.8, 1.0 Hz), 7.80 (2H, dd, J = 6.7, 1.9 Hz), 7.95-7.87 (2H, m), 8.40 (2H, dd, J = 6.7, 1.9 Hz), 8.61 (1H, d, J = 5.0 Hz), 8.66 (1H, dq, J = 5.0, 0.9 Hz).492490 2-98 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 2.48 (3H, s), 5.37 (2H, s), 7.23 (1H, d, J = 7.3 Hz), 7.28 (1H, d, J = 7.3 Hz), 7.48-7.45 (3H, m), 7.56 (1H, d, J = 7.9 Hz), 7.64-7.59 (2H, m), 7.67 (1H, d, J = 8.6 Hz), 7.82-7.72 (4H, m), 8.35 (2H, dd, J = 6.8, 2.0 Hz).539537

TABLE 2-122-99 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 2.41 (3H, s), 5.30 (2H, s), 7.09 (1H, d, J = 7.7 Hz), 7.14 (1H, d, J = 7.7 Hz), 7.51-7.45 (3H, m), 7.54 (1H, d, J = 7.7 Hz), 7.60 (1H, t, J = 7.7 Hz), 7.66-7.64 (3H, m), 7.78 (1H, d, J = 8.2 Hz), 7.81 (1H, dd, J = 8.2, 0.8 Hz), 8.61 (1H, dd, J = 8.2, 0.8 Hz), 9.40-9.39 (1H, m), 13.78 (1H, br s).540538 2-100 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 2.50 (3H, s), 5.41 (2H, s), 7.29-7.24 (1H, m), 7.35 (2H, dd, J = 4.9, 1.1 Hz), 7.43-7.39 (2H, m), 7.56 (2H, dd, J = 7.7, 4.2 Hz), 7.67 (1H, d, J = 8.6 Hz), 7.73 (2H, d, J = 8.6 Hz), 7.86-7.78 (2H, m), 8.36 (2H, d, J = 8.6 Hz), 8.59-8.57 (1H, m).539537 2-101 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 2.34 (3H, s), 5.41 (2H, s), 7.27 (1H, d, J = 7.7 Hz), 7.34 (1H, t, J = 7.7 Hz), 7.43-7.39 (4H, m), 7.56 (1H, d, J = 7.7 Hz), 7.68 (1H, d, J = 8.4 Hz), 7.72 (2H, dd, J = 6.7, 1.9 Hz), 7.87-7.78 (2H, m), 8.35 (2H, dd, J = 6.7, 1.9 Hz), 8.59 (1H, d, J = 4.4 Hz).539537 2-102 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 2.35 (3H, s), 5.40 (2H, s), 7.27 (2H, d, J = 7.9 Hz), 7.42-7.37 (2H, m), 7.49 (2H, d, J = 7.9 Hz), 7.56 (1H, d, J = 7.9 Hz), 7.67 (1H, d, J = 8.6 Hz), 7.71 (2H, d, J = 8.6 Hz), 7.85-7.77 (2H, m), 8.34 (2H, d, J = 8.6 Hz), 8.58 (1H, dq, J = 4.9, 0.9 Hz).539537 2-103 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 5.39 (2H, s), 7.39- 7.35 (2H, m), 7.56 (1H, d, J = 7.9 Hz), 7.69- 7.64 (2H, m), 7.72 (2H, dd, J = 6.7, 1.9 Hz), 7.83-7.75 (3H, m), 7.89-7.85 (2H, m), 8.39 (2H, dd, J = 6.7, 1.9 Hz), 8.57 (1H, d, J = 4.9 Hz).593591 2-104 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 5.40 (2H, s), 7.40- 7.36 (2H, m), 7.56 (1H, d, J = 7.9 Hz), 7.67 (1H, d, J = 8.6 Hz), 7.72 (1H, d, J = 7.9 Hz), 7.84-7.76 (5H, m), 7.92 (1H, d, J = 7.7 Hz), 7.99 (1H, br s), 8.37 (2H, dd, J = 6.8, 2.0 Hz), 8.57 (1H, dq, J = 4.9, 0.9 Hz).593591 2-105 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 5.40 (2H, s), 7.41- 7.36 (2H, m), 7.56 (1H, d, J = 7.9 Hz), 7.68 (1H, d, J = 8.6 Hz), 7.84-7.77 (8H, m), 8.37 (2H, dd, J = 6.7, 1.9 Hz), 8.57 (1H, d, J = 4.9 Hz).593591 2-106 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 5.40 (2H, s), 7.41- 7.36 (2H, m), 7.51-7.45 (3H, m), 7.57 (1H, d, J = 7.7 Hz), 7.64-7.60 (2H, m), 7.68 (1H, d, J = 8.4 Hz), 7.84-7.79 (3H, m), 8.10 (1H, dd, J = 10.5, 1.4 Hz), 8.19 (1H, dd, J = 8.2, 1.5 Hz), 8.57 (1H, dq, J = 4.9, 0.9 Hz).543541 2-107 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 2.54 (3H, s), 5.45 (2H, s), 7.30 (1H, td, J = 7.6, 1.1 Hz), 7.44- 7.35 (3H, m), 7.56-7.50 (1H, m), 7.58 (1H, d, J = 7.9 Hz), 7.71-7.67 (2H, m), 7.75 (2H, dd, J = 6.7, 1.9 Hz), 7.81 (1H, t, J = 7.9 Hz), 7.92 (1H, br s), 8.36 (2H, dd, J = 6.7, 1.9 Hz).557555

TABLE 2-132-108 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 2.52 (3H, s), 5.43 (2H, s), 7.33 (1H, d, J = 7.7 Hz), 7.38 (1H, d, J = 7.7 Hz), 7.43 (1H, td, J = 7.7, 1.4 Hz), 7.48 (1H, td, J = 7.7, 1.9 Hz), 7.57 (1H, d, J = 8.0 Hz), 7.63 (1H, dd, J = 8.0, 1.2 Hz), 7.68 (1H, d, J = 8.3 Hz), 7.76-7.72 (3H, m), 7.81 (1H, t, J = 8.3 Hz), 7.90-7.84 (1H, m), 8.37 (2H, dd, J = 6.7, 1.9 Hz).573571 2-109 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 2.51 (3H, s), 3.88 (3H, s), 5.41 (2H, s), 7.00 (1H, td, J = 7.5, 0.9 Hz), 7.12 (1H, d, J = 7.9 Hz), 7.29 (1H, d, J = 7.9 Hz), 7.34 (1H, d, J = 7.5 Hz), 7.43 (1H, ddd, J = 8.8, 7.1, 1.3 Hz), 7.52 (1H, dd, J = 7.5, 1.7 Hz), 7.57 (1H, d, J = 7.9 Hz), 7.69- 7.66 (3H, m), 7.85-7.78 (2H, m), 8.34 (2H, dd, J = 6.8, 2.0 Hz).569567 2-110 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 2.24 (3H, s), 2.49 (3H, s), 5.41 (2H, s), 7.22 (1H, br s), 7.31 (1H, br s), 7.46-7.43 (3H, m), 7.60-7.57 (3H, m), 7.72-7.68 (3H, m), 7.82 (1H, t, J = 8.3 Hz), 8.33 (2H, dd, J = 6.7, 1.8 Hz).553551 2-111 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 5.42 (2H, d, J = 1.6 Hz), 7.42-7.48 (4H, m), 7.54 (1H, t, J = 4.2 Hz), 7.59-7.63 (2H, m). 7.64-7.73 (3H. m), 7.81 (2H, d, J = 4.2 Hz), 8.29 (2H, d, J = 8.6 Hz), 8.35-8.37 (1H, m).543541 2-112 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 5.37 (2H, s), 7.37 (1H, d, J = 8.3 Hz), 7.44-7.48 (3H, m), 7.55 (1H, d, J = 7.6 Hz), 7.58-7.66 (3H, m), 7.73 (2H, d, J = 8.6 Hz), 7.79 (1H, t, J = 8.1 Hz), 7.88 (1H, dd, J = 8.6, 2.3 Hz), 8.34 (2H, d, J = 8.6 Hz), 8.59 (1H, d, J = 2.3 Hz).559557 2-113 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 2.27 (3H, s), 5.43 (2H, s), 7.35 (1H, s), 7.39 (1H, d, J = 5.8 Hz). 7.44-7.48 (3H, m), 7.57-7.63 (3H, m), 7.69- 7.75 (3H, m), 7.83 (1H, t, J = 8.1 Hz), 8.35 (2H, d, J = 8.3 Hz), 8.51 (1H, d, J = 5.3 Hz).539537 2-114 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 7.36 (1H, d, J = 7.9 Hz), 7.44-7.48 (3H, m), 7.56 (1H, d, J = 7.9 Hz), 7.59-7.63 (2H, m), 7.66-7.75 (4H, m), 7.79 (1H, t, J = 8.1 Hz), 8.34 (2H, d, J = 8.6 Hz), 8.44 (1H, s).539537 2-115 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 5.36 (2H, s), 7.41 (1H, dd, J = 8.8, 4.4 Hz), 7.44-7.48 (3H, m), 7.55 (1H, d, J = 7.9 Hz), 7.58-7.63 (2H, m), 7.65-7.75 (4H, m), 7.79 (1H, t, J = 8.1 Hz), 8.34 (2H, d, J = 8.6 Hz), 8.53 (1H, d, J = 3.0 Hz).543541 2-116 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 2.53 (3H, s), 5.44 (2H, s), 7.54-7.30 (6H, m), 7.58 (1H, d, J = 7.7 Hz), 7.69 (1H, d, J = 8.0 Hz), 7.74 (2H, dd, J = 6.8, 1.8 Hz), 7.81 (1H, t, J = 8.0 Hz), 7.90 (1H, br s), 8.35 (2H, dd, J = 6.8, 1.8 Hz).557555

TABLE 2-142-117 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 2.51 (3H, s), 5.42 (2H, s), 7.33-7.29 (3H, m), 7.37 (1H, d, J = 7.9 Hz), 7.57 (1H, d, J = 7.9 Hz), 7.69-7.65 (3H, m), 7.72 (2H, dd, J = 6.8, 1.8 Hz), 7.89- 7.78 (2H, m), 8.34 (2H, dd, J = 6.8, 1.8 Hz).557555 2-118 [Image Omitted]

1H-NMR (CDCl3) Î´: 3.87 (3H, s), 5.27 (2H, s), 6.61 (1H, d, J = 8.2 Hz), 6.86 (1H, d, J = 7.3 Hz), 7.31 (1H, d, J = 8.6 Hz), 7.35-7.39 (3H, m), 7.43-7.51 (2H, m), 7.53-7.60 (3H, m), 7.64 (2H, d, J = 8.4 Hz), 8.50 (2H, d, J = 8.4 Hz).555553 2-119 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 5.10 (2H, s), 6.16- 6.32 (2H, m), 7.32-7.38 (1H, m), 7.44-7.48 (3H, m), 7.54-7.63 (4H, m), 7.70-7.75 (2H, m), 7.77-7.84 (1H, m), 8.34 (2H, d, J = 8.6 Hz).541539 2-120 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 3.89 (3H, s), 5.48 (2H, s), 7.20-7.26 (2H, m), 7.44-7.49 (3H, m), 7.58-7.64 (3H, m), 7.69-7.75 (3H, m), 7.84 (1H, t, J = 8.2 Hz), 8.33 (2H, d, J = 8.6 Hz), 8.56 (1H, d, J = 6.0 Hz).555553 2-121 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 5.47 (2H, s), 7.44- 7.48 (3H, m), 7.55-7.63 (4H, m), 7.68 (1H, d, J = 8.6 Hz), 7.73 (2H, d, J = 8.6 Hz), 7.78- 7.84 (2H, m), 8.03 (1H, t, J = 8.1 Hz), 8.35 (2H, d, J = 8.6 Hz), 13.58 (1H, br s).593591 2-122 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 5.47 (2H, s), 7.44- 7.51 (4H, m), 7.56-7.63 (3H, m), 7.67-7.74 (4H, m), 7.82 (1H, t, J = 8.1 Hz), 8.34 (2H, d, J = 8.6 Hz), 8.83 (1H, d, J = 5.1 Hz).593591 2-123 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 2.50 (3H, s), 5.40 (2H, s), 7.29 (1H, d, J = 7.7 Hz), 7.33 (1H, d, J = 7.7 Hz), 7.59-7.46 (4H, m), 7.68 (1H, d, J = 8.4 Hz), 7.71-7.69 (1H, m), 7.75 (2H, dd, J = 6.7, 1.9 Hz), 7.84-7.78 (2H, m), 8.36 (2H, dd, J = 6.7, 1.9 Hz).573571 2-124 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 2.49 (3H, s), 5.39 (2H, s), 7.26 (1H, d, J = 7.5 Hz), 7.31 (1H, d, J = 7.5 Hz), 7.53 (2H, dt, J = 8.7, 2.2 Hz), 7.56 (1H, d, J = 7.9 Hz), 7.63 (2H, dt, J = 8.7, 2.2 Hz), 7.67 (1H, d, J = 8.4 Hz), 7.74 (2H, dd, J = 6.7, 1.9 Hz), 7.83-7.76 (2H, m), 8.35 (2H, dd, J = 6.7, 1.9 Hz).573571 2-125 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 2.50 (3H, s), 3.80 (3H, s), 5.40 (2H, s), 7.03 (1H, dq, J = 8.6, 1.2 Hz), 7.19-7.14 (2H, m), 7.29 (1H, d, J = 7.3 Hz), 7.39-7.32 (2H, m), 7.57 (1H, d, J = 7.9 Hz), 7.67 (1H, d, J = 8.6 Hz), 7.73 (2H, dd, J = 6.7, 1.9 Hz), 7.85-7.77 (2H, m), 8.34 (2H, dd, J = 6.7, 1.9 Hz).569567

TABLE 2-152-126 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 2.49 (3H, s), 3.81 (3H, s), 5.40 (2H, s), 7.01 (2H, dt, J = 9.5, 2.4 Hz), 7.28 (1H, d, J = 6.8 Hz), 7.32 (1H, d, J = 7.5 Hz), 7.57-7.53 (3H, m), 7.70-7.66 (3H, m), 7.80 (2H, t, J = 7.9 Hz), 8.33 (2H, dd, J = 6.8, 2.0 Hz).569567 2-127 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 2.35 (3H, s), 2.51 (3H, s), 5.42 (2H, s), 7.27 (2H, d, J = 7.7 Hz), 7.32 (1H, d, J = 7.7 Hz), 7.36 (1H, d, J = 7.7 Hz), 7.49 (2H, d, J = 8.2 Hz), 7.57 (1H, d, J = 7.7 Hz), 7.72-7.66 (3H, m), 7.88-7.77 (2H, m), 8.33 (2H, dd, J = 6.7, 1.9 Hz).556551 2-128 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.19 (3H, t, J = 7.5 Hz), 2.52 (3H, s), 2.65 (2H, q, J = 7.6 Hz), 5.43 (2H, s), 7.30 (2H, d, J = 8.4 Hz), 7.34 (1H, d, J = 7.7 Hz), 7.39 (1H, d, J = 7.7 Hz), 7.52 (2H, d, J = 8.4 Hz), 7.57 (1H, d, J = 7.7 Hz), 7.72-7.67 (3H, m), 7.81 (1H, t, J = 8.3 Hz), 7.87 (1H, br s), 8.33 (2H, dd, J = 6.7, 1.9 Hz).567565 2-129 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 2.51 (3H, s), 5.42 (2H, s), 7.32 (1H, d, J = 7.1 Hz), 7.37 (1H, d, J = 7.7 Hz), 7.63-7.48 (4H, m), 7.72-7.67 (3H, m), 7.89-7.77 (3H, m), 8.37 (2H, dd, J = 6.8, 2.0 Hz).623621 2-130 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 2.55 (3H, s), 5.47 (2H, s), 7.40 (1H, d, J = 7.1 Hz), 7.48-7.43 (3H, m), 7.58 (1H, d, J = 7.7 Hz), 7.69 (1H, d, J = 8.5 Hz), 7.77-7.72 (4H, m), 7.82 (1H, t, J = 8.5 Hz), 7.95 (1H, br s), 8.35 (2H, dd, J = 6.7, 1.9 Hz).623621

TABLE 3-1ExampleStructureNMRMS(M + H)MS(M âˆ’ H)Note3-1 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 2.19 (6H, s), 2.30 (3H, s), 7.01 (2H, s), 7.90 (2H, d, J = 8.4 Hz), 8.51 (2H, d, J = 8.4 Hz), 13.26 (1H, br s).360358 3-2 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 3.72 (3H, s), 4.59 (2H, d, J = 5.5 Hz), 5.41 (1H, t, J = 5.5 Hz), 7.45 (1H, d, J = 8.4 Hz), 7.67 (1H, d, J = 8.4 Hz), 7.92 (2H, d, J = 8.4 Hz), 8.53 (2H, d, J = 8.4 Hz), 13.70 (1H, br s).412410 3-3 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 2.31 (3H, s), 3.71 (3H, s), 7.36 (1H, d, J = 8.4 Hz), 7.49 (1H, d, J = 8.4 Hz), 7.93 (2H, d, J = 8.3 Hz), 8.53 (2H, d, J = 8.3 Hz), 13.67 (1H, br s).396394 3-4 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.09 (3H, t, J = 7.0 Hz), 2.30 (3H, s), 3.94 (2H, q, J = 7.0 Hz), 7.34 (1H, d, J = 8.2 Hz), 7.48 (1H, d, J = 8.2 Hz), 7.93 (2H, d, J = 8.2 Hz), 8.53 (2H, d, J = 8.2 Hz), 13.66 (1H, br s).410408 3-5 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.91 (3H, s), 3.74 (3H, s), 4.32 (2H, d, J = 5.8 Hz), 7.43 (1H, d J = 8.4 Hz), 7.49 (1H, d, J = 8.4 Hz), 7.92 (2H, d, J = 8.4 Hz), 8.42 (1H, t, J = 5.8 Hz), 8.53 (2H, d, J = 8.4 Hz), 13.73 (1H, br s).453451 3-6 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 2.17 (3H, s), 4.55 (2H, d, J = 5.1 Hz), 5.35 (1H, t, J = 5.2 Hz), 7.48 (1H, d, J = 8.4 Hz), 7.60 (1H, d, J = 8.4 Hz), 7.91 (2H, d, J = 8.6 Hz), 8.51 (2H, d, J = 8.6 Hz), 13.61 (1H, br s).396394 3-7 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.90 (3H, s), 2.21 (3H, s), 4.28 (2H, d, J = 5.7 Hz), 7.41 (1H, d, J = 8.4 Hz), 7.47 (1H, d, J = 8.4 Hz), 7.91 (2H, d, J = 8.2 Hz), 8.37 (1H, t, J = 5.8 Hz), 8.51 (2H, d, J = 8.2 Hz), 13.62 (1H, br s).437435 3-8 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.16 (9H, s), 2.21 (3H, s), 4.27 (2H, d, J = 5.7 Hz), 7.34 (1H, d, J = 8.4 Hz), 7.46 (1H, d, J = 8.4 Hz), 7.91 (2H, d, J = 8.2 Hz), 8.09 (1H, t, J = 5.6 Hz), 8.51 (2H, d, J = 8.2 Hz), 13.64 (1H, br s).479477

TABLE 3-23-9 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 2.32 (3H, s), 3.47 (2H, t, J = 4.9 Hz), 3.88 (2H, t, J = 4.8 Hz), 4.70 (1H, br s), 7.34 (1H, d, J = 8.1 Hz), 7.47 (1H, d, J = 8.1 Hz), 7.92 (2H, d, J = 8.4 Hz), 8.51 (2H, d, J = 8.4 Hz), 13.56 (1H, br s).426424 3-10 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 1.33 (9H, s), 2.22 (3H, s), 4.30 (2H, d, J = 6.0 Hz), 7.52-7.54 (2H, m), 7.60 (1H, d, J = 8.4 Hz), 7.91 (2H, d, J = 8.4 Hz), 8.51 (2H, d, J = 8.4 Hz), 13.65 (1H, br s).515513 3-11 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 3.79-3.87 (3H, m), 7.28 (1H, dd, J = 9.3, 3.7 Hz), 7.67 (1H, t, J = 9.2 Hz), 7.93 (2H, d, J = 8.6 Hz), 8.51 (2H, d, J = 8.6 Hz), 13.68 (1H, br s).400398 3-12 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 3.82 (3H, s), 5.22 (2H, s), 7.17 (2H, d, J = 9.0 Hz), 7.24-7.27 (1H, br m), 7.35 (1H, t, J = 7.2 Hz), 7.41 (2H, t, J = 7.3 Hz), 7.47 (2H, d, J = 7.1 Hz), 7.63- 7.66 (1H, br m), 8.28 (2H, d, J = 9.0 Hz), 13.26 (1H, br s).438436 3-13 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 2.36 (3H, s), 5.01 (2H, s), 7.11-7.15 (1H, m), 7.22 (1H, d, J = 7.7 Hz), 7.39 (1H, d, J = 8.4 Hz), 7.52 (1H, d, J = 8.4 Hz), 7.60 (1H, td, J = 7.7, 1.7 Hz), 7.86 (2H, d, J = 8.4 Hz), 8.31 (1H, d, J = 4.0 Hz), 8.41 (2H, d, J = 8.4 Hz), 13.61 (1H, br s).473471 3-14 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.88 (6H, d, J = 6.6 Hz), 1.41 (6H, s), 1.83-1.95 (1H, m), 2.19 (3H, s), 2.54 (2H, d, J = 7.3 Hz), 4.33 (2H, d, J = 5.5 Hz), 7.28-7.38 (3H, m), 7.46 (1H, d, J = 8.4 Hz), 8.24 (2H, d, J = 8.2 Hz), 8.52-8.60 (1H, m), 13.35 (1H, br s).521519 3-15 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.88 (6H, d, J = 6.6 Hz), 1.18 (3H, t, J = 7.1 Hz), 1.83-1.94 (1H, m), 2.19 (3H, s), 2.54 (2H, d, J = 7.1 Hz), 4.02 (2H, q, J = 7.1 Hz), 4.21 (2H, d, J = 5.7 Hz), 7.33 (2H, d, J = 8.4 Hz), 7.39 (1H, d, J = 8.2 Hz), 7.46 (1H, d, J = 8.2 Hz), 7.69-7.75 (1H, m), 8.24 (2H, d, J = 8.4 Hz), 13.33 (1H, br s).455453 3-16 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.91 (3H, t, J = 7.3 Hz), 1.37 (6H, s), 1.60-1.67 (2H, m), 2.33 (3H, s), 2.62 (2H, t, J = 7.6 Hz), 4.31 (2H, d, J = 5.7 Hz), 7.30-7.38 (3H, m), 7.52 (1H, s), 8.10-8.15 (2H, m), 8.48 (1H, t, J = 5.7 Hz).507505

TABLE 3-33-17 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.35 (2H, dt, J = 8.0, 2.9 Hz), 0.59 (2H, ddd, J = 9.1, 5.0, 2.9 Hz), 1.20-1.29 (1H, m), 1.38 (6H, s), 2.35 (3H, s), 3.93 (2H, d, J = 7.0 Hz), 4.32 (2H, d, J = 5.6 Hz), 7.05 (2H, d, J = 8.8 Hz), 7.44 (1H, s), 7.56 (1H, s), 8.29 (2H, d, J = 8.8 Hz), 8.49 (1H, t, J = 5.6 Hz), 13.08 (1H, br s).535533 3-18 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.99 (6H, d, J = 6.6 Hz), 1.41 (6H, s), 1.98-2.10 (1H, m), 2.18 (3H, s), 3.85 (2H, d, J = 6.6 Hz), 4.32 (2H, d, J = 5.7 Hz), 7.06 (2H, d, J = 8.4 Hz), 7.30 (1H, d, J = 8.2 Hz), 7.44 (1H, d, J = 8.2 Hz), 8.27 (2H, d, J = 8.4 Hz), 8.52-8.59 (1H, m), 13.23 (1H, br s).537535 3-19 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.35 (2H, td, J = 5.2, 4.1 Hz), 0.57-0.62 (2H, m), 1.24-1.27 (1H, m), 2.61 (3H, s), 3.94 (2H, d, J = 7.2 Hz), 7.09 (2H, d, J = 8.8 Hz), 7.64 (1H, s), 8.27 (1H, s), 8.30 (2H, d, J = 8.8 Hz), 13.20 (2H, br s).412410 3-20 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.35 (2H, td, J = 5.1, 4.1 Hz), 0.58-0.60 (2H, m), 1.22-1.30 (1H, m), 2.45 (3H, s), 3.94 (2H, d, J = 7.2 Hz), 7.09 (2H, d, J = 9.1 Hz), 7.56 (2H, s), 7.81 (1H, s), 7.85 (1H, s), 8.32 (2H, d, J = 8.8 Hz), 13.06 (1H, br s).411409 3-21 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.35 (2H, td, J = 5.2, 4.0 Hz), 0.57-0.62 (2H, m), 1.22-1.27 (1H, m), 2.42 (3H, s), 2.77 (3H, d, J = 4.7 Hz), 3.94 (2H, d, J = 7.0 Hz), 7.09 (2H, d, J = 9.1 Hz), 7.56 (1H, s), 7.77 (1H, s), 8.31 (2H, d, J = 9.1 Hz), 8.32 (1H, s), 13.09 (1H, br s).425423 3-22 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.35 (2H, td, J = 5.6, 4.4 Hz), 0.57-0.61 (2H, m), 1.21-1.29 (1H, m), 2.28 (3H, s), 2.81 (3H, s), 3.02 (3H, s), 3.93 (2H, d, J = 7.2 Hz), 7.08 (2H, d, J = 9.1 Hz), 7.59 (1H, s), 7.62 (1H, s), 8.30 (2H, d, J = 9.1 Hz), 13.10 (1H, br s).439437 3-23 [Image Omitted]

1H-NMR (DMSO-D6) Î´: 0.35 (2H, td, J = 5.3, 4.0 Hz), 0.57-0.61 (2H, m), 0.90 (3H, t, J = 7.3 Hz), 1.20-1.30 (2H, m), 1.30-1.40 (2H, m), 1.46-1.53 (2H, m), 2.41 (3H, s), 3.24 (2H, q, J = 6.9 Hz), 3.94 (2H, d, J = 7.2 Hz), 7.09 (2H, d, J = 9.1 Hz), 7.56 (1H, s), 7.74 (1H, s), 8.31 (2H, d, J = 9.1 Hz), 8.37 (1H, t, J = 5.5 Hz), 13.09 (1H, br s).467465

Experimental Example 1Evaluation of Human mPGES-1 Enzyme Inhibitory Activity

The human mPGES-1 enzyme inhibitory activity of a test article was evaluated according to the report of Xu et al. (XU, D et al. MF63 [2-(6-chloro-1H-phenanthro[9,10-d]imidazol-2-yl)-isophthalonitrile], a selective microsomal prostaglandin E synthase-1 inhibitor, relieves pyresis and pain in preclinical models of inflammation. J Pharmacol Exp Ther. Sep. 2008, Vol. 326, No. 3, pages 754-763). That is, the amount of PGE2 produced by human mPGES-1 in the presence of a test article was measured by the HTRF (homogeneous time resolved fluorescence) method, based on which the human mPGES-1 enzyme inhibitory activity of the test article was determined.

1) Preparation of Human mPGES-1 Expressing Cell Microsome Fraction

A DNA fragment containing human mPGES-1, which is added with a BamHI recognition cleavage sequence immediately before the translation initiation codon and an EcoRI recognition cleavage sequence immediately after the translation termination codon was amplified by the PCR (Polymerase Chain Reaction) method using a human mPGES-1 expression plasmid DNA (pME-18S/iPGES-1) prepared in-house as a template. The purified DNA fragment was digested with BamHI and EcoRI, and ligated to pcDNA3.1(+) (Invitrogen, model number V790-20), similarly digested with BamHI and EcoRI, by using a DNA Ligation kit ver. 2.1 (Takara Bio, model number 6022). The human mPGES-1 expression plasmid DNA was isolated from Escherichia coli DH5Î± (TOYOBO, model number DNA-903) transformed with the obtained ligation product. The base sequence of human mPGES-1 cloned to a vector was determined by the Dye Terminator method by using BigDye Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems, #4337455). The determined sequence was identical with the sequence of the protein translational region of human mPGES-1 (Accession number NM 004878) registered in the NCBI Reference Database.

Human mPGES-1 expression plasmid DNA was transfected into Chinese hamster ovary-derived cells (FreeStyle CHO-S Cell, Invitrogen, #R800-07) by using a transgene reagent (FreeStyle MAX Reagent (Invitrogen, #16447-100)), and cultured with shaking (8% CO₂, 37Â° C.) in a medium containing 8 mmol/L L-glutamine (GIBCO FreeStyle CHO Expression Medium, Invitrogen, #12651-022) for 48 hr.

The CHO-S cells were suspended in Homogenate Buffer (100 mmol/L potassium phosphate (pH 7.4), 250 mmol/L Sucrose, 100 mmol/L EDTA, complete EDTA free (Roche, #1873580)). Using an ultrasonic disruptor UD-201 (Tomy Seiko), the suspended cells were disrupted at output: 3, duty cycle: 50 for 30 seconds. The precipitate was removed by centrifugation (1,000Ã—g, 5 min, 4Â° C.), and the supernatant was centrifuged (5,000Ã—g, 10 min, 4Â° C.) The supernatant was further centrifuged (105,000Ã—g, 60 min, 4Â° C.). The obtained precipitate was suspended in Resuspension Buffer (100 mmol/L potassium phosphate (pH 7.4), 250 mmol/L sucrose, 100 mmol/L EDTA, 10% glycerol) to give a microsome fraction.

The protein concentration of the microsome fraction was measured by the Bradford method (Protein Assay Kit, Bio-Rad). The microsome fraction was rapidly frozen in liquid nitrogen and preserved at âˆ’80Â° C. Human mPGES-1 in the microsome fraction was detected by Western Blot using rabbit anti-mPGES-1 polyclonal antibody (ThermoFisher Scientific, #PA1-10264).

2) Evaluation of Human mPGES-1 Enzyme Inhibitory Activity

A test article solution diluted with 0.1 mol/L potassium phosphate, pH 7.4 (hereinafter to be referred to as KPB) or DMSO (Nacalai Tesque, #13407-45) was added at 5 Î¼L/well to 96 well V-bottom plate (Corning, #3363). The final DMSO concentration during the reaction was set to 2%(v/v). Furthermore, a microsome fraction of CHO-S cells expressing human mPGES-1, which was diluted with reduced GSH (12.5 mmol/L KPB solution, SIGMA, #G6529-25G) such that the protein concentration was 5 Î¼g/mL, was added at 20 Î¼L/well. The amount of the microsome fraction used is the amount of microsome fraction within a range where the amount of PGE2 produced under the reaction conditions shown below and the amount of microsome fraction used show linearity. To the blank was added reduced GSH (12.5 mmol/L KPB solution) at 20 Î¼L/well. After stirring at room temperature for 10 min, PGH2 (PGH2 dissolved in cold acetone to 100 Î¼g/mL and diluted with D-PBS(âˆ’) (Nikken biomedical laboratory, #CM6201) to 10 Î¼g/mL, Cayman Chemical, #17020) was added at 25 Î¼L/well, and the mixture was stood at room temperature for 45 seconds. Tin(II) chloride dihydrate (2 mg/mL 10 mmol/L citric acid solution, Wako Pure Chemical Industries, Ltd., #204-01562) was added at 50 Î¼L/well, and the plate was gently shaken to discontinue the enzyme reaction.

The concentration of PGE2 in the above-mentioned enzyme reaction mixture was measured using Prostaglandin E2 assay (CISbio Bioassays, #62P2APEC) according to the manual. As the reference standard for analytical curve, PGE2 (Cayman Chemical, #14010) was used. Using RUBYstar (BMG Labtech), the time-resolved fluorescence at 620 nm and 665 nm relative to the excitation light at 337 nm was measured. PGE2 concentration was extrapolated from the PGE2 analytical curve. Average of the PGE2 concentrations of the respectively-treated wells was used as the data.

The mPGES-1 enzyme inhibitory activity (%) of the test article was calculated according to the following formula 1.

mPGES-1 enzyme inhibitory activity(%)=(PGE2_Aâˆ’PGE2_x)/(PGE2_Aâˆ’PGE2_B)Ã—100â€ƒâ€ƒ[formula 1]

PGE2_A: PGE2 concentration of vehicle-treated well
PGE2_B: PGE2 concentration of blank well
PGE2_x: PGE2 concentration of test article-treated well

The IC₅₀value (50% inhibitory concentration) of the test article was calculated according to the following formula 2.

IC₅₀value=10^{{log 10(D/E)Ã—(}50âˆ’G)/(Fâˆ’G)+log 10(E)}â€ƒâ€ƒ[formula 2]

D: concentration of test article that shows activity of not less than 50% inhibition between two points across 50% inhibition
E: concentration of test article that shows activity of not more than 50% inhibition between two points across 50% inhibition
F: mPGES-1 enzyme inhibitory activity (%) when concentration of test article is D
G: mPGES-1 enzyme inhibitory activity (%) when concentration of test article is E

The results are shown in Table 4-1 to Table 4-9.

TABLE 4-1Human mPGES-1enzyme inhibitoryExampleactivity (Î¼M)1-10.8131-20.1381-30.1641-40.0251-50.6721-60.1631-70.6521-827.01-90.6011-105% inhibition(at 30 Î¼M)1-1142% inhibition(at 30 Î¼M)1-120.0151-130.3971-141.4131-150.00741-160.0101-170.7351-180.1141-190.4111-200.00161-210.9881-220.00271-230.1341-240.00061-250.1081-260.0181-270.00101-280.00061-290.00111-300.00061-310.00101-320.00071-330.00081-340.00591-350.0221-360.00071-370.00081-380.00151-390.00191-402.2311-410.00231-420.00101-430.00201-440.00061-450.1381-460.00071-470.0431-480.00091-490.00091-500.00091-510.0010

TABLE 4-2Human mPGES-1enzyme inhibitoryExampleactivity (Î¼M)1-520.00061-530.00451-540.00091-550.00111-560.00061-570.00051-580.00051-590.00061-600.00041-610.00071-620.00101-630.00051-640.00191-650.00861-660.00411-670.00101-680.00031-690.00031-700.00071-710.00251-720.00131-730.00061-740.00061-750.00671-760.00171-770.00091-780.00221-790.00121-800.00311-810.00061-820.00081-830.0111-840.00061-850.00051-860.00581-870.00081-880.00121-890.00091-900.00041-910.00041-920.00051-930.00071-940.00461-950.00211-960.0811-970.00911-980.00091-990.00071-1000.00091-1010.00581-1020.0009

TABLE 4-3Human mPGES-1enzyme inhibitoryExampleactivity (Î¼M)1-1030.00131-1040.00151-1050.00071-1060.00071-1070.00121-1080.00061-1090.00251-1100.00091-1110.00121-1120.00091-1130.00091-1140.00591-1150.00061-1160.00201-1170.00161-1180.00191-1190.00101-1200.0481-1210.00121-1220.00131-1230.00231-1240.00091-1250.00081-1260.00081-1270.00081-1280.00131-1290.00061-1300.0091-1310.00091-1320.00031-1330.00051-1340.0041-1350.00051-1360.00051-1370.00051-1380.00051-1390.00061-1400.00051-1410.00111-1420.00051-1430.00101-1440.00091-1450.00051-1460.00041-1470.00081-1480.00171-1490.00081-1500.00041-1510.00041-1520.00041-1530.0005

TABLE 4-4Human mPGES-1enzyme inhibitoryExampleactivity (Î¼M)1-1540.00051-1550.00351-1560.00411-1570.00071-1580.0121-1590.00071-1600.00141-1610.00131-1620.00121-1630.00101-1640.00371-1650.00091-1660.00111-1670.00191-1680.00211-1690.00201-1700.00151-1710.00051-1720.0171-1730.00541-1740.00311-1750.00131-1760.00181-1770.00131-1780.00141-1790.0161-1800.00411-1810.00241-1820.00161-1830.00131-1840.00191-1850.00171-1860.00141-1870.00531-1880.00161-1891.11-1900.0471-1910.0151-19241% inhibition(at 30 Î¼M)1-1938.71-19410.51-1956.01-1960.0421-1970.2891-1980.0141-1990.0311-2000.0101-2010.3061-2020.00821-2030.0201-2040.034

TABLE 4-5Human mPGES-1enzyme inhibitoryExampleactivity (Î¼M)1-2050.3671-2060.0141-2070.00431-2080.0161-2090.0591-2100.2881-2110.0631-2120.0321-2130.0881-2140.0241-2150.4521-2160.0391-2170.1261-2180.0701-2190.0411-2200.0161-2210.0791-2220.1651-2230.0071-2245.81-2254.21-2262.21-2270.0501-2280.6721-2290.5321-2300.7501-2310.0451-2320.5211-2330.8481-2341.01-2350.0701-2360.2631-2371.31-2380.00741-2390.4281-2400.4281-2410.2781-2420.0821-2430.1201-2440.0211-2450.1081-2460.3071-2470.0111-2480.0161-2490.2261-2500.0121-2510.0181-2520.5111-2530.7911-2540.0301-2550.045

TABLE 4-6Human mPGES-1enzyme inhibitoryExampleactivity (Î¼M)1-2560.0981-2570.0171-2581.91-2590.1761-2600.1471-26144% inhibition(at 30 Î¼M)1-2620.0071-2630.7021-2640.1631-2650.0561-2660.0111-2670.1502-10.2832-221.32-314.72-40.0662-50.1012-628% inhibition(at 30 Î¼M)2-73.52-81.92-96.42-100.0732-110.00602-120.1412-1323.12-1414.32-1516.42-160.4122-170.0392-180.00802-190.2112-200.0522-210.3412-220.2192-230.1552-242.42-250.2492-262.72-277.72-283.72-290.5032-3043% inhibition(at 30 Î¼M)2-310.0312-320.0142-330.1022-340.1632-350.0172-360.0532-370.0412-381.02-390.450

TABLE 4-7Human mPGES-1enzyme inhibitoryExampleactivity (Î¼M)2-401.32-410.4292-420.2392-430.5702-440.5632-450.0122-460.4942-470.2952-480.0192-490.0142-500.0612-510.0902-520.1002-530.0112-540.1702-550.0102-560.0182-570.0252-580.1452-590.0952-600.1212-610.0922-620.00932-630.2592-640.0122-650.1512-660.0162-670.0272-680.6722-690.0842-700.1582-710.1722-720.2832-730.4022-740.4242-750.00372-760.00582-770.00372-780.00682-790.00372-800.00162-810.00272-820.00172-830.0512-840.0172-850.00162-860.00222-870.00182-880.00202-890.0182-902.1

TABLE 4-8Human mPGES-1enzyme inhibitoryExampleactivity (Î¼M)2-910.00162-920.00442-930.0382-940.00412-950.00502-960.0212-970.00892-980.00372-990.00172-1000.00562-1010.00652-1020.00592-1030.0102-1040.0182-1050.0182-1060.00502-1070.00382-1080.00642-1090.00282-1100.00742-1110.00442-1120.00592-1130.00512-1140.00492-1150.00302-1160.00552-1170.00472-1180.00712-1190.00182-1200.00412-1210.0142-1220.0152-1230.0122-1240.0122-1250.00432-1260.00462-1270.00622-1280.0132-1290.0322-1300.0353-10.4843-20.1483-30.1413-40.2023-50.3413-60.0563-70.0193-80.00103-90.7763-100.0163-110.093

TABLE 4-9Human mPGES-1enzyme inhibitoryExampleactivity (Î¼M)3-120.0283-130.0343-140.0023-150.00513-160.00213-170.00083-180.00123-1924.63-201.13-210.6113-226.93-230.041

Experimental Example 2Evaluation of Action of mPGES-1 Inhibitor on Normal Intraocular Pressure of Macaca fascicularis

This test was performed using male Macaca fascicularis. To eliminate interindividual difference and an influence of the difference in administration days, a crossover test was used for the evaluation as shown in Table 5.

TABLE 5Animal No.First courseSecond courseThird courseSX1M01test articlereference articlevehicle10 mg/kgSX1M02reference articlevehicletest article30 mg/kgSX1M03reference articlevehicletest article30 mg/kgSX1M04vehicletest articlereference article30 mg/kgSX1M05vehicletest articlereference article30 mg/kg

To exclude the influence of the remaining test article, a 1-week washout period was set between tests. On the day of test, the monkeys were fed after the final measurement.

The test article (compound of Examples 2-98) was suspended in 0.5% methylcellulose (Wako Pure Chemical Industries, Ltd.), and administered by gavage by using a polypropylene syringe (sterilized disposable product, Nipro Corporation) and a stomach catheter (nelaton type A No. 9, Izumo health). The dose was set to 10 mg/kg/5 mL (N=1) or 30 mg/kg/5 mL (N=4) based on the body weight of each individual the day before the administration. To the vehicle group was administered the vehicle (0.5% methylcellulose (MC)) by a method similar to that for the test article. As a reference article, Xalatan (registered trade mark) ophthalmic solution 0.005% (Pfizer Inc., general name: latanoprost) was used. The reference article was administered by instillation of 20 Î¼L thereof to one eye by using a micropipette. After instillation, the lacrimal part was lightly fixed by gently pressing the lower eyelid for about 15 seconds. The opposite eye was treated in the same manner. The intraocular pressure was measured immediately before administration, and 2, 4, 8, 12 and 24 hr after administration. Before measurement of the intraocular pressure, the animal was fixed on a monkey chair, and topically anesthetized by instillation of an ophthalmic surface anesthetic (Benoxyl (registered trade mark) ophthalmic solution 0.4%, Santen Pharmaceutical Co., Ltd., general name: oxybuprocaine hydrochloride). A lid rectactor (Handaya Co., Ltd.) was set, and the intraocular pressure of the both eyes was measured using a pneumatic applanation tonometer (Model30 Classic, Reichert Inc.).

To confirm disappearance of the test article, after an intraocular pressure measurement at 24 hr after the third course administration, blood samples (1 mL) were collected from the femoral vein under unanesthetized condition by using polypropylene syringe and 23 gauge injection needle (both sterilized disposable products) treated with heparin sodium, and the concentration of unaltered compound in the plasma containing the test article was measured.

An intraocular pressure difference (Î”mmHg; in first decimal place) from the value immediately before administration was determined for each measurement eye at each measurement time point, an average of the both eyes was calculated and taken as the evaluation data of the individual. The mean and standard deviation (in second decimal place) of the intraocular pressure difference was calculated for each group, and the test article administration group or reference article administration group was subjected to a homoscedasticity test (significance level 5%) based on F-test with the vehicle group. When the dispersion was equal, Student's t-test was performed and, when the dispersion was not equal, Aspin-Welch's t-test was performed. In addition, the maximum ocular hypotensive effect (Î”mmHg; maximum descent value from value immediately before administration, in first decimal place) was determined for each group, and the groups were compared in the same manner. The two-sided test was performed. It is a significant variation when a difference from the vehicle group was found at a 5% significance level and shown in FIG. 1 separately as 5% and 1%. Since the test article 10 mg/kg administration group contained only one animal, it was excluded from the statistical analysis.

The intraocular pressure of Macaca fascicularis used for this test before administration of a test article was 19.6Â±1.7 mmHg. After the measurement of intraocular pressure at 24 hr after the third course administration, the concentration of an unaltered test article in the plasma of the vehicle group and the reference article administration group was less than the lower detection limit. The results are shown in FIG. 1.

Experimental Example 3Evaluation of Effect on Prostaglandin Composition in Guinea Pig Aqueous Humor

A test article was dissolved in saline containing 0.5% polysorbate80 (Fluka) and 0.003% ophthalmic solution (pH 7.0âˆ’8.0) was prepared. The test article was administered to Hartley male guinea pig by instillation of 20 Î¼L thereof to one eye by using a micropipette. After instillation, the lacrimal part was lightly fixed by gently pressing the lower eyelid for about 15 seconds. The opposite eye was treated in the same manner. To the vehicle group was administered the medium (0.5% polysorbate-containing saline) by a method similar to that for the test article. After 23 hr from the instillation, Mydrin P (registered trade mark) 0.5% ophthalmic solution (Santen Pharmaceutical Co., Ltd., general name: tropicamide/phenylephrine hydrochloride) was dropwisely added by one drop to the both eyes of a guinea pig to cause mydriasis. The guinea pig was anesthetized with Escain (registered trade mark) inhalation anesthetics (Pfizer Inc., general name: isoflurane), the cornea of the both eyes was tapped with a 30 G injection needle, and the leaked aqueous humor (primary aqueous humor) was collected. One hour later (24 hr after instillation), the guinea pig was anesthetized again with isoflurane, and the secondary aqueous humor was collected in the same manner. The concentration of prostaglandins in the secondary aqueous humor obtained from each group (4 guinea pigs, 8 eyes) was measured by the LC/MS/MS system (Ultra high performance liquid chromatography: Nexera (registered trademark) manufactured by Shimadzu Corporation, mass spectrometer: AB SCIEX manufactured by QTRAP (registered trademark) 5500), and the concentration ratio of each prostaglandin concentration relative to the total of all prostaglandin concentrations was calculated. The results are shown in Table 6.

TABLE 6Exam-6-keto-plePGE2 (%)PGF2Î± (%)PGF1Î± (%)PGD2 (%)TXB2 (%)vehicle80.876.84.70.71-5150.71421.713.20.41-81609.815.713.21.31-983814.231.216.30.31-10929.514.137.518.90.11-12237.311.727.7230.21-12836.213.929.719.30.81-12962.510.218.19.201-13073.6811.26.211-13142.99.827.918.80.61-13556.112.719.410.90.91-13666.77.917.37.40.71-13749.511.324.814.10.31-150698.91480.21-16928.713.540.3170.51-178301336.620.10.31-1845710.321.210.70.81-185501125.412.11.62-9837.814.827.320.10

Experimental Example 4Evaluation of Action of mPGES-1 Inhibitor on Normal Intraocular Pressure of Macaca fascicularis

This test is performed using male Macaca fascicularis. To eliminate interindividual difference and an influence of the difference in administration days, a crossover test is used for the evaluation as shown in Table 7.

TABLE 7Animal No.First courseSecond courseThird courseFourth courseSX1M01vehicletest articletest article +reference articlereference articleSX1M02test articlevehiclereference articletest article +reference articleSX1M03reference articletest article +test articlevehiclereference articleSX1M04test article +test articlevehiclereference articlereference articleSX1M05vehiclereference articletest article +test articlereference articleSX1M06test articlevehiclereference articletest article +reference article

To exclude the influence of the remaining test article, a 1-week washout period is set between tests. On the day of test, the monkeys are fed after the final measurement.

A test article is dissolved in saline containing 0.5% polysorbate80 (Fluka) and 0.1% ophthalmic solution (pH 7.9âˆ’8.1) is prepared. To the vehicle group is administered the medium (0.5% polysorbate-containing saline) by a method similar to that for the test article. As a reference article, Xalatan (registered trademark) ophthalmic solution 0.005% (Pfizer Inc., general name: latanoprost) is used. The test article is administered by instillation of 30 Î¼L thereof to one eye 5 times and 1 time of vehicle at 5-min intervals by using a micropipette (total 6 times instillation for each eye). Each of vehicle and reference article is adiministered 1 time and then vehicle is instilled 5 times (total 6 times instillation for each eye). In the test article+reference article combination group, the test article is instilled 5 times after instillation of the reference article (total 6 times instillation for each eye). After instillation at each time, the lacrimal part is lightly fixed by gently pressing the lower eyelid for about 15 seconds. The intraocular pressure is measured immediately before administration, and 2, 4, 8, 12 and 24 hr after administration. Before measurement of the intraocular pressure, the animal is fixed on a monkey chair, and topically anesthetized by instillation of an ophthalmic surface anesthetic (Benoxyl (registered trademark) ophthalmic solution 0.4%, Santen Pharmaceutical Co., Ltd., general name: oxybuprocaine hydrochloride). A lid rectactor (Handaya Co., Ltd.) is set, and the intraocular pressure of the both eyes is measured using a pneumatic applanation tonometer (Model30 Classic, Reichert Inc.).

An intraocular pressure difference (Î”mmHg; in first decimal place) from the value immediately before administration is determined for each measurement eye at each measurement time point, an average of the both eyes is calculated and taken as the evaluation data of the individual. The mean and standard deviation (in second decimal place) of the intraocular pressure difference is calculated for each group, and the test article administration group or reference article administration group is subjected to a homoscedasticity test (significance level 5%) based on F-test with the vehicle group. When the dispersion is equal, Student's t-test is performed and, when the dispersion is not equal, Aspin-Welch's t-test is performed. In addition, the maximum ocular hypotensive effect (Î”mmHg; maximum descent value from value immediately before administration, in first decimal place) is determined for each group, and the groups are compared in the same manner. The two-sided test is performed. It is a significant variation when a difference from the vehicle group is found at a 5% significance level.

The Formulation Examples of the present invention include the following formulations. However, the present invention is not limited by such Formulation Examples.

Formulation Example 1Production of Capsule

1) compound of Example 1-8630 mg2) microcrystalline cellulose10 mg3) lactose19 mg4) magnesium stearateâ€‚1 mg

1), 2), 3) and 4) are mixed and filled in a gelatin capsule.

Formulation Example 2Production of Tablet

1) compound of Example 8610 g2) lactose50 g3) cornstarch15 g4) carmellose calcium44 g5) magnesium stearateâ€‚1 g

The total amount of 1), 2), 3) and 30 g of 4) are kneaded with water, vacuum dried and sieved. The sieved powder is mixed with 14 g of 4) and 1 g of 5), and the mixture is tableted by a tableting machine. In this way, 1000 tablets containing 10 mg of the compound of Example 1-86 per tablet are obtained.

Formulation Example 3Production of Eye Drop

in 100 mL of eye drop1) compound of Example 1-86100 mg2) polysorbate80500 mg3) sodium chloride900 mg4) sodium hydroxideq.s.5) sterilized purified waterq.s.

The above components are aseptically blended to pH 7.9âˆ’8.1 to give an eye drop.

Formulation Example 4Production of Eye Drop

in 100 mL of eye drop1) compound of Example 1-86100 mg2) polysorbate80100 mg3) sodium dihydrogen phosphate dehydrate100 mg4) sodium chloride900 mg5) benzalkonium chlorideâ€ƒ5 mg6) sodium hydroxideq.s.7) sterilized purified waterq.s.

The above components are aseptically blended to pH 7.9âˆ’8.1 to give an eye drop.

Formulation Example 5Production of Eye Drop

in 100 mL of eye drop1) compound of Example 1-86100 mg2) boric acid700 mg3) boraxq.s.4) sodium chloride500 mg5) sodium edetate0.05 mgâ€‰6) benzalkonium chloride0.0005 mgâ€ƒâ€‰7) sterilized purified waterq.s.

The above components are aseptically blended to pH 7.9âˆ’8.1 to give an eye drop.

INDUSTRIAL APPLICABILITY

Since the compound of the present invention and a pharmaceutically acceptable salt thereof have an mPGES-1 inhibitory activity, they can afford a medicament effective for the prophylaxis or treatment of pain, rheumatism, osteoarthritis, fever, Alzheimer's disease, multiple sclerosis, arteriosclerosis, glaucoma, ocular hypertension, ischemic retinal disease, systemic scleroderma, cancer including colorectal cancer and diseases for which suppression of PGE2 production is effective.

This application is based on a patent application No. 2014-031035 filed in Japan on Feb. 20, 2014, the contents of which are incorporated in full herein.