The fluid may be administered to reduce or prevent or delay onset of ocular discomfort such as itchiness or ocular pain associated with severe ocular surface disease. The ocular pain may be associated with acute or chronic inflammation, or immune response. With the reduction of the pain, comes the reduction of secondary neuroinflammatory effects (Belmonte C et al., “TFOS DEWS II pain and sensation report”, The Ocular Surface, 15:404-437). The cause of the pain may be known or unknown.

In some embodiments, the severe ocular surface disease is severe dry eye. In other embodiments, the severe ocular surface disease is a severe ocular surface disease other than severe dry eye.

In some embodiments, the severe ocular surface disease is one or more listed in the Table 1.

TABLE 1

Examples of severe ocular surface disease

Swelling of the cornea
Hayes, S. et al, “The structural response of the cornea to

changes in stromal hydration”, J.R. Soc. Interface,

2017, 14: 1-9.

Congential corneal opacities
Mataftsi, A. et al., “Chromosome abnormalities and the

genetics of congenital corneal opacification”, Molecular

Vision, 2011, 17: 1624-1640.

Nischal, K. K., “Genetics of Congenital Corneal

Opacification-Impact on Diagnosis and Treatment,”

Cornea, 2015, 34 Suppl 10: S24-34.

Corneal scars
Rose J. S. et al., “Objective quantification of corneal

haziness using anterior segment optical coherence

tomography, Journal of Current Ophthalmology, 2018,

30(1): 54-57.

Torricelli, A. A. M. et al., “The corneal fibrosis response

to epithelial-stromal injury,” Exp Eye Res., 2016,

142: 110-118.

Giant papillary conjunctivitis
Dunn, Jr., J. P. et al., “Giant papillary conjunctivitis

associated with elevated corneal deposits, Cornea, 1990,

(4): 357-8.

Cicatricial Pemphigoid
Ebrahimiadib, N. et al., “Atopy in Patients With Ocular

Cicatricial Pemphigoid,” Cornea, 2018, 37(4): 436-441.

Corneal haze with Impaired
Liu, Y. L. et al., “Visual performance after excimer laser

visual performance after laser
photorefractive keratectomy for high myopia,” Taiwan J

treatment
Ophthalmol, 2017, 7(2): 82-88.

Severe ocular surface disease
Javadi, M-A. et al., “Chronic and Delayed-Onset

after Chemical bum
Mustard Gas Keratitis, Report of 48 Patients and Review

of Literature, Ophthalmology, 2005, 112: (4): 1-11.

Baradaran-Rafii, A., “Mustard Gas Induced Ocular

Surface Disorders”, Journal of Ophthalmic and Vision

Research, 2013, 8(4): 383-390.

Xerophthalmia
Rosen, D. S. et al., “Vitamin A deficiency and

xerophthalmia in western Yemen,” Eur J Clin Nutr.,

1996, 50(1): 54-7.

Trachoma with corneal
Whitcher , J. P., et al. , “Corneal blindness: a global

scarring and vascularization
perspective”, Bull World Health Organ, 2001, 79(3):

214-21.

Corneal scars after viral
Bowman, R. J. et al. “Non-trachomatous corneal opacities

infections (measles)
in the Gambia-aetiology and visual burden, Eye (Lond),

2002, 16(1): 27-32.

In some embodiments, the severe ocular surface disease is one or more selected from among the following, as identified by TCD coding:

- ICD-10-CM Codes>H00-H59 Diseases of the eye and adnexa>H15-H22
- Disorders of sclera, cornea, iris and ciliary body>
- Other disorders of cornea H18->
- Codes
- H00-H05 Disorders of eyelid, lacrimal system and orbit
- H10-H11 Disorders of conjunctiva
- H17.9 Corneal scars and opacifications, unspecified
- H17.1 other central corneal opacifications
- H17.8 specified corneal scars and opacifications
- Corneal Haze with:
- H15-H22 Disorders of sclera, cornea, iris and ciliary body
- H53-H54 Visual disturbances and blindness
- H55-H57 Other disorders of eye and adnexa
- H59-H59 Intraoperative and post-procedural complications and disorders of eye and adnexa, not elsewhere classified.

Optionally, the fluid further includes one or more bioactive agents (e.g., a hydrophobic active ingredient). As used herein, the term “bioactive agent” refers to any substance that has an effect on the human or non-human animal subject when administered in an effective amount to affect the tissue. The bioactive agent may be any class of substance such as a drug molecule or biologic (e.g., polypeptide, carbohydrate, glycoprotein, immunoglobulin, nucleic acid), may be natural products or artificially produced, and may act by any mechanism such as pharmacological, immunological, or metabolic. Examples of classes of bioactive agents include substances that modify the pressure of the eye (e.g., enzyme inhibitors) and anti-angiogenic agents. Some specific examples of bioactive agents include steroids (e.g., corticosteroids), antibiotics, immunosuppressants, immunomodulatory agents, tacrolimus, plasmin activator, anti-plasmin, and cyclosporin A. In some embodiments, the bioactive agent is a steroid or antibiotic to treat or prevent eye infection; glaucoma drug such as prostaglandin analog, beta blocker, alpha agonist, or carbonic anhydrase inhibitor; agent for allergy eye relief such as histamine antagonist or non-steroidal anti-inflammatory drug; or mydriatic agent. Unfortunately, in some cases, the bioactive agent or agents included in the fluid may be irritative or damaging to the eye (e.g., cyclosporin A). Advantageously, through its rheological property and other properties, the high molecular weight HA in the fluid can alleviate and/or protect the eye from the irritative and/or damaging effects of the biologically active agent or agents within the fluid (i.e., the bioactive agent would be more irritative or more damaging to the eye if administered without the high molecular weight HA).

In some embodiments, the fluid contains no steroid, antibiotic or immunomodulator. In some embodiments, the fluid contains no other bioactive agent (e.g., no hydrophobic active ingredient).

In some circumstances, it may be desirable to include one or more preservatives or detergents within the fluid. Often, such preservatives and detergents are irritative or damaging to the eye. Advantageously, through its rheological property and other properties, the fluid can alleviate and/or protect the eye from the irritative and/or damaging effects of the preservative or detergent within the fluid. Thus, in some embodiments, the fluid further comprises a preservative or detergent that is irritative or damaging to the eye (i.e., a preservative or detergent that would be more irritative or more damaging to the eye if administered without the high molecular weight HA). In some embodiments, the fluid contains no preservative or detergent.

In some embodiments, the fluid includes cyclosporin A, cetalkoniumchloride, tyloxapol, or a combination of two or more of the foregoing.

In some embodiments, the fluid is administered to the subject before, during, and/or after administration of another composition comprising a bioactive agent to the subject. In some circumstances, it may be desirable to include one or more preservatives or detergents within the other composition. As indicated above, often, such preservatives and detergents are irritative or damaging to the eye, and some bioactive agents themselves may be irritative or damaging to the eye. Advantageously, through its rheological property and other properties, the fluid can alleviate and/or protect the eye from the irritative and/or damaging effects of the bioactive agent, preservative, and/or detergent within the other composition. Thus, the bioactive agent, preservative, and/or detergent within the other composition would be more irritative or more damaging to the eye if administered without the fluid.

In some embodiments, the other composition includes one or more of an antibiotic, immunosuppressant, or immunomodulatory agent.

In some embodiments, the other composition includes cyclosporin A, cetalkoniumchloride, tyloxapol, or a combination of two or more of the foregoing.

The other composition administered to the subject may be in any form and administered by any route (e.g., local or systemic). In some embodiments, the other composition is administered to the eye, e.g., topically or by injection. In some embodiments, the other composition is topically administered to the ocular surface.

In some embodiments, the preservative or detergent included in the fluid or other composition is a chemical preservative or oxidative preservative.

In some embodiments, the preservative or detergent included in the fluid or other composition is one that kills susceptible microbial cells by disrupting the lipid structure of the microbial cell membrane, thereby increasing microbial cell membrane permeability.

In some embodiments, the preservative or detergent included in the fluid or other composition is one that causes damage to the corneal tissues, such as corneal epithelium, endothelium, stroma, and interfaces such as membranes.

In some embodiments, the preservative or detergent included in the fluid or other composition is selected from the group consisting of quaternary ammonium preservative (e.g., benzalkonium chloride (BAK) or cetalkoniumchloride), chlorobutanol, edetate disodium (EDTA), polyquaternarium-1 (e.g., Polyquad™ preservative), stabilized oxidizing agent (e.g., stabilized oxychloro complex (e.g., Purite™ preservative)), ionic-buffered preservative (e.g., sofZia™ preservative), polyhexamethylene biguanide (PHMB), sodium perborate (e.g., GenAqua™ preservative), tylopaxol, and sorbate.

In some embodiments, the fluid is at least essentially mucin-free; or in other words having a mucin concentration of <0.3% w/v.

In some embodiments, the fluid includes a preservative. In other embodiments, the fluid does not include a preservative (i.e., the fluid is preservative-free).

In some embodiments, the fluid further includes a glycosaminoglycan (GAG), i.e., includes one or more GAGs in addition to the high molecular weight HA; electrolyte (e.g., sodium chloride); buffer (e.g., phosphate buffer); or a combination of two or more of the foregoing.

In some embodiments, the severe ocular surface disease is severe dry eye. In other embodiments, the severe ocular surface disease is a severe ocular surface disease other than severe dry eye.

The subject may or may not have dry eye syndrome (the aqueous tear deficiency type or qualitative dry eye type) at the time the fluid is administered to the eye of the subject. In some embodiments of the therapeutic or prophylactic methods, the eye of the subject to which the fluid is administered does not have aqueous tear deficiency (ATD) at the time of administering the fluid (i.e., in the absence of ATD). In some embodiments of the therapeutic or prophylactic methods, the eye of the subject to which the fluid is administered does not have qualitative dry eye at the time of administering the fluid (i.e., in the absence of qualitative dry eye). In some embodiments of the therapeutic or prophylactic methods, the eye of the subject to which the fluid is administered does not have dry eye syndrome at the time of administering the fluid (i.e., in the absence of aqueous tear deficiency or qualitative dry eye).

In some embodiments of the therapeutic or prophylactic methods, the subject is not suffering from a tear volume deficiency; however, the subject has an ocular surface abnormality (a topographic anomaly) comprising elevations on the cornea or elsewhere on the eye surface for which the normal tear film (tear film of normal surface tension and viscosity) does not cover, resulting in areas of friction at the ocular surface (van Setten, Epitheliopathy of the bleb (EoB)-identifying attrition: A new model for failure of glaucoma surgery, New Frontiers in Ophthalmology, 2018: 4(3): 1-4).

The fluid may be used in conjunction with a bandage contact lens. Thus, the method may further include applying a bandage contact lens to the eye before, during, and/or after administering the fluid. For example, the fluid may be administered before applying the bandage contact lens, after the contact lens, and/or placing fluid on the bandage contact lens before applying the bandage contact lens to the eye. Use of the fluid allows the bandage contact lens to exert pressure on the ocular surface while simultaneously minimizing friction at the ocular surface. Advantageously, the fluid and bandage contact lens can safely be used shortly after ocular surgery, e.g., glaucoma surgery.

Another aspect of the invention concerns a kit that may be used for carrying out the method of the invention described herein, i.e., reducing or delaying the onset of severe ocular surface disease. The kit comprises the fluid described herein, and one or more bandage contact lenses. Bandage contact lenses may be packaged together with the fluid within the same container (with the bandage contact lenses in contact with the fluid), or the bandage contact lenses may be separate from the fluid, packaged in separate containers. Suitable containers include, for example, bottles, vials, syringes, blister pack, etc. The containers may be formed from a variety of materials such as glass or plastic.

The kit may include a delivery agent (separately or in association with the fluid) that is to be brought into contact with the ocular surface or other part of the eye. For example, the kit may include particles (e.g., microparticles or nanoparticles) that are coated with the fluid and/or release the fluid onto the ocular surface.

Optionally, the kit may include a device for dispensing eye drops (e.g., an eye dropper), which may or may not serve as a container for the fluid in the kit before the kit's outer packaging is accessed (e.g., opened), i.e., the eye drop dispensing device may function to contain the fluid provided in the unaccessed (unopened) kit, or may be empty and receive the fluid after the kit is accessed. Optionally, the kit may include a label or packaging insert with printed or digital instructions for use of the kit, e.g., for carrying out the method of the invention.

Kits can include packaging material that is compartmentalized to receive one or more containers such as vials, tubes, and the like, each of the container(s) including one of the separate elements to be used in a method described herein. Packaging materials for use in packaging pharmaceutical products include, by way of example only U.S. Pat. Nos. 5,323,907, 5,052,558 and 5,033,252. Examples of pharmaceutical packaging materials include, but are not limited to, blister packs, bottles, tubes, pumps, bags, vials, light-tight sealed containers, syringes, bottles, and any packaging material suitable for a selected formulation and intended mode of administration and treatment.

A kit may include one or more additional containers, each with one or more of various materials desirable from a commercial and user standpoint for use of the compositions described herein. Non-limiting examples of such materials include, but not limited to, buffers, diluents, carrier, package, container, vial and/or tube labels listing contents and/or instructions for use, and package inserts with instructions for use.

A label can be on or associated with the container. A label can be on a container when letters, numbers or other characters forming the label are attached, molded or etched into the container itself; a label can be associated with a container when it is present within a receptacle or carrier that also holds the container, e.g., as a package insert. A label can be used to indicate that the contents are to be used for a specific therapeutic application. The label can also indicate directions for use of the contents, such as in the methods described herein.

In some embodiments of the kit, the fluid can be presented in a pack or dispenser device which can contain one or more unit dosage forms containing a composition disclosed herein. The pack can for example contain metal or plastic foil, such as a blister pack. The pack or dispenser device can be accompanied by instructions for administration.

Corneal Opacity

The severe OSD may be a corneal opacity. The fluid can enhance transparency (reduce opacity) partly or entirely, and prevent or delay onset of desiccation of the ocular surface. Advantageously, the fluid can avoid the need for a nurse to add water to the eye during an examination or procedure.

The corneal opacity may involve complete opacity or partial opacity, and may involve the entire ocular surface, or a portion of the ocular surface.

The corneal opacity may have one or more of a variety of causative or contributing agents. In some embodiments, the corneal opacity is caused by corneal edema, such edema caused by glaucoma; birth injury; or Fuchs dystrophy.

In some embodiments, the corneal opacity is caused by corneal scarring.

In some embodiments, the corneal opacity comprises a congenital corneal opacity. The congenital corneal opacity may be caused, for example, by birth trauma; Peters anomaly; dermoid tumor (limbal dermoid); sclerocornea; congenital hereditary endothelial dystrophy (CHED); mucopolysaccharidoses; congenital glaucoma, microphthalmia, metabolic disease infection; or inflammation.

In some embodiments, the corneal opacity is caused by a lack of blinking. For example, the lack of blinking may be due to a birth defect in a child that lacks the blinking reflex. In some embodiments, subject has Stevens-Johnson Syndrome.

In some embodiments, the corneal opacity is caused by inflammation; sensitivity to non-infectious bacteria; ulcer on the eye; infection; keratitis; trachoma; onchocerciasis (River blindness); dryness of the eye due to Sjogren syndrome, vitamin A deficiency, LASIK, LASEK, or PRK eye surgery; dystrophy (inherited metabolic disease); keratoconus; or injury to the eye (e.g., chemical burn, welding injury, etc.).

Optionally, the fluid may be administered in combination with another intervention for treatment of the corneal opacity or treatment of the cause of the opacity (e.g., antibiotic, steroid, phototherapeutic keratectomy (PTK), cornea transplant). The other intervention may be administered before, during or after administration of the fluid. If the other intervention involves administration of a bioactive agent, the bioactive agent may be included in the fluid or administered in a separate composition by a route suitable for the bioactive agent.

Acute and Chronic Ocular Surface Inflammation

Acute or chronic ocular surface inflammation may cause or contribute to a severe ocular surface disease. Ocular inflammation may take the form of numerous eye disorders of varying severity depending on the location of the inflammation. OSDs that may be attributed to ocular inflammation include, but are not limited to, uveitis, conjunctivitis, episcleritis, scleritis, optic neuritis, retrobulbar neuritis, keratitis, blepharitis, and the like. Many of these conditions can occur secondary to an infection of the eye, such as a bacterial, viral, fungal, or protozoan (e.g., amoeba) infection. Ocular inflammation can also result following ophthalmologic surgical procedures or ocular trauma resulting from physical injury of the eye.

Inflammation is a normal component of the immune response and a necessary component of the healing process, but is detrimental if it proceeds at an inappropriate level or duration. An acute leucocytic inflammation is triggered within hours of exposure to the causative agent (e.g., antigen, wound to the eye, or other insult) and represents a normal, time-limited inflammatory reaction. The end of this phase varies, but reduced inflammation provides an indirect sign of improvement and slow reconstitution to a prior state.

In contrast to acute ocular inflammation, chronic ocular inflammation is a state that is not merely different quantitatively in duration (i.e., prolonged), but also qualitatively different in kind. The differences between acute ocular inflammation and chronic ocular inflammation are significant, analogous to the differences between the immunological events in acute, healing wounds and chronic, non-healing wounds (see, for example, Tarnuzzer R W and Schultz G S, “Biochemical analysis of acute and chronic wound environments,” Wound Repair Regen., 1996, July-September, 4(3):321-325). These differences in immunological events, particularly at conjunctival and subconjunctival tissue (as opposed to the cornea), underlie a diversity of ocular surface disorders and explain why some patients within a disease or disorder population are refractory to an otherwise effective treatment plan.

Ocular surface homeostasis is regulated by resident lymphocytes (CD8+ T cells, gamma delta T cells, and natural killer T cells) and CD4+ regulatory T cells, among other factors. These interact with anti-inflammatory factors, such as interleukin (IL)-1 receptor antagonist, transforming growth factor (TGF)-beta 2, and matrix protease inhibitors such as inhibitor of metalloproteinase (TIMP)-1. Stress factors including environment challenges, infections, endogenic stress, autoimmunity and genetic factors may all disturb the finely-tuned homeostatic balance existing on the ocular surface and activate an acute inflammatory response. The method of the invention provides a holistic approach to establishing, restoring, and preserving ocular surface homeostasis, and thereby reduce or delay the onset of severe ocular surface disease. Thus, before or after onset, topical administration of the fluid can result in attenuation and long-term alleviation of the severe ocular surface disease.

Epithelial-derived pro-inflammatory cytokines activate immature resident antigen-presenting cells (APCs), which are mainly dendritic cells, on the ocular surface. Mature APCs migrate to the regional lymph nodes and initiate an adaptive immune response by priming naïve CD4+ T cells including T helper (Th)1 and Th17 cells. Through activated angiogenesis and lymphangiogesis, these inflammatory mediators traffic back to the ocular surface, where Th1-secreted interferon (IFN)-gamma and Th17-secreted IL-17 increased cytokine production, induce epithelial and goblet cell apoptosis and alter conjunctival homeostasis, perpetuating a chronic inflammatory process. In a state of goblet cell deficiency, the deprivation of gel-forming mucins leads to increased friction, thus perpetuating inflammation (Pflugfelder S C et al., “Epithelial-immune cell interaction in dry eye,” Cornea, 2008, 27 Suppl 1:S9-11).

Whereas acute ocular surface inflammation is characterized by a rapid onset and relatively shorter duration (typically, a few minutes to a number of days), protein exudate, and the cellular infiltrate of the conjunctival and subconjunctival tissue is mainly neutrophils, chronic ocular surface inflammation is characterized by a longer duration (a number of days to years), fewer neutrophils, the cellular infiltrate is mainly macrophages and lymphocytes, and proliferation of blood vessels, fibrosis and tissue necrosis. Indeed, the primary cells of chronic ocular surface inflammation are macrophages and lymphocytes at the conjunctiva and subconjunctiva.

Typically, chronic ocular surface inflammation can also be identified by ocular surface inflammation markers including one or more of: tear film hyperosmolarity; elevated matrix metalloproteinase-9 (MMP-9) expression by epithelial cells and infiltrating leucocytes at the ocular surface; increased MMP-9 activity at the ocular surface (its MMP-3 and TIMP-1-mediated enzymatic activity); elevated interferon-gamma; over-expression of human leukocyte antigen-antigen D related (HLA-DR) by conjunctival epithelial cells; and intracellular adhesion molecule 1 (ICAM-1) by conjunctival epithelial cells, increased number of activated lymphocytes, and decreased number of goblet cells.

Steroids (e.g., corticosteroids) are considered the gold standard therapy for ocular inflammations. Dexamethasone, prednisolone, triamcinolone acetonide, fluocinolone acetonide and loteprednol etabonate are commonly employed, which act by various mechanisms such as inhibition of multiple inflammatory cytokines, fibrin deposition, polymorphonuclear leukocyte migration, and anti-angiogenesis effects by inhibition of nuclear factor-kappa-B (NF-kB) signal pathway (Nakao et al., Am J Pathol. 2007; 171(3):1058-65).

The anti-inflammatory action of steroids is thought to be due to interference with arachidonic acid metabolism, i.e., by inhibition of phospholipase A2 which causes the release of arachidonic acid from the tissue phospholipid pool. Although steroids are effective in the treatment of ocular inflammation, their extended use is complicated by severe side effects, the most common of which are cataract formation, glaucoma, and infectious eye diseases (Kymionis G D et al., “Treatment of chronic dry eye: treatment of cyclosporine,” Clin Ophthalmol. 2008 December; 2(4): 829-836; Schultz C, “Safety and efficacy of cyclosporine in the treatment of chronic dry eye,” Ophthalmol Eye Dis. 2014; 6: 37-42). Likewise, immunomodulatory drugs such as cyclosporine and anti-inflammatory agents such as tetracycline and its derivatives can cause ocular pain and irritation in some patients (e.g., burning and itching), which can be difficult to tolerate in an inflamed eye.

Advantageously, topical administration of high molecular weight hyaluronic acid (HA), e.g., 3-4 MDa, may be used as an effective natural immunomodulator to regain ocular surface immune homeostasis in patient groups with chronic ocular surface inflammation or seasonal allergic keratoconjunctivitis, without the aforementioned agents or their side effects. In particular, fluids of the invention may be used to restore immune homeostasis of the ocular surface in these patients. Furthermore, other immunomodulatory agents may be administered to the subject before, during, or after administration of the high molecular weight HA, and the high molecular weight HA can make the environment at the ocular surface more conducive to the immunomodulatory agent's activity, enhancing or facilitating the action of the immunomodulatory agent.

The influence of the molecular weight of the HA molecules in eye drops on their bio-chemical function has very recently come into the focus of ophthalmic research. Due to its function in the extracellular matrix, HA plays an important role in the proliferation and differentiation of keratinocytes, in corneal wound healing, and in the homeostasis of the corneal epithelium. The prevalence of dry eyes in elderly persons is likely to be associated with the significant decrease of free HA in the extracellular matrix starting from the fifth decade. Moreover, HA acts as a signal molecule. Whereas, high molecular weight HA (3-4 MDa) acts anti-angiogenic and immunosuppressive, HA of medium molecular weight has inflammatory, immunostimulating, as well as angiogenic properties (Stern R et al., “Hyaluronan fragments: An information-rich system”, European Journal of Cell Biology, 2006, 85:699-715; and Noble P W, “Hyaluronan and its catabolic products in tissue injury and repair,” Matrix Biology, 2002, 21:25-29). Elevated enzyme levels in chronic inflammation cause increased hydrolysis of HA and thus a shift toward lower molecular weight. High molecular weight HA in eye drops can contribute to minimize this inflammation-related shift by decreasing friction at the ocular surface and supporting the down-regulation of the inflammatory process. A decrease of friction may also lead to a decrease of nociceptive neurological stimuli that can trigger the maintenance or onset of neurogenic inflammation supportive pathways.

It is well known that during acute and chronic inflammation various putative mediators of inflammation are released by the inflamed tissues and by leukocytes. The concentrations of these mediators and leukocytes are indicative of the level or degree of inflammation. Likewise, a reduction in concentration of these mediators and leukocytes is an indication of the effectiveness of a drug in treating inflammation. Examples of ocular surface inflammation markers include, but are not limited to, those listed above, i.e., tear film hyper-osmolarity; elevated matrix metalloproteinase-9 (MMP-9) expression by epithelial cells and infiltrating leucocytes at the ocular surface; increased MMP-9 activity at the ocular surface (its MMP-3 and TIMP-1-mediated enzymatic activity); elevated interferon-gamma; over-expression of human leukocyte antigen-antigen D related (HLA-DR) by conjunctival epithelial cells; and intracellular adhesion molecule 1 (ICAM-1) by conjunctival epithelial cells, increased number of activated lymphocytes, and decreased number of goblet cells.

Optionally, before administration of the fluid of the invention, the method of the invention may further comprise identifying the subject/eye as having chronic ocular surface inflammation by detecting or measuring one or more of these inflammatory markers of the ocular surface. Optionally, the method may further comprise detecting or measuring one or more of these inflammatory markers of the ocular surface one or more times after administration of the fluid of the invention in order to monitor the inflammatory status of the eye(s) over time and guide the frequency and duration of further administration of the fluid of the invention.

Fluid Preparation

As indicated above, the hyaluronic acid of the fluid has an intrinsic viscosity of greater than 2.5 m³/kg and a concentration of <0.2% w/v. In some embodiments, the hyaluronic acid has an intrinsic viscosity of greater than 2.9 m³/kg.

Viscoelasticity is defined as characteristics of a fluid having both viscous and elastic properties. The zero shear viscosity is determined as the steady shear plateau viscosity at vanishing shear rate. For highly viscous formulations, measurement with a controlled stress rheometer is preferred.

The relation between molecular weight and intrinsic viscosity [7] in m³/kg is given through the Mark-Houwink equation:

[η]=k·(M_rm)a

with M_rmbeing the molecular mass in MDa

and the coefficients

k=1.3327·10⁻⁴

and

a=0.6691

which values for k and a having been found as most predictive.

The fluid may be produced by: sterilizing the filling line; adding purified water or water for injection (WFI) to a stainless steel mixing tank; adding salts while mixing; slowly adding HA and mixing until a homogeneous solution/fluid is achieved; adjusting pH value by adding NaOH or HCl, if required, while continuing the mixing process; transferring the solution over a 1 μm pore size filter cartridge to a sterile holding tank; and aseptically filling the solution via sterile filtration into the sterile primary package (monodose or vial). In the case of monodoses, this may be done by a blow-fill-seal (BFS) process.

Preferably, the fluid has at least essentially mucin-free or in other words having a mucin concentration of <0.3% w/v. This means that the flow behavior or properties essentially is reached or adjusted by hyaluronan and not by mucin naturally present in the subject's tear fluid and mainly responsible for the flow behavior thereof.

It is preferred that if substances are added that increase the viscosity, they are added towards, or during, or as a final step. The mixing is carried out so as to reach a homogeneous mixture. As an alternative or in addition, it is preferred to initially provide purified water or water for injection as a basis, and then, optionally, electrolytes, buffers and substances which do not increase the viscosity are added at first to the purified water or water for injection.

HA is further described in the monograph of the European Pharmacopoeia 9.0, page 3583 (Sodium Hyaluronate), which is incorporated herein by reference in its entirety.

In one embodiment, the fluid used in the method and kit of the invention has the characteristics listed in Table 2:

TABLE 2

Characteristic
Specification
Test Method

Appearance
clear and colorless solution,
Ph. Eur.

free from visible impurities

pH value
6.8 -
7.6
Ph. Eur.

Osmolality
240 -
330 mosmol/kg
Ph. Eur.

HA concentration
0.10 -
0.19% w/v
Ph. Eur.

NaCl concentration
7.6 -
10.5 g/l
Ph. Eur.

Sterility
Sterile
Ph. Eur.

Phosphate concentration
1.0 -
1.4 mmol/l
Ph. Eur.

Exemplified Embodiments

Embodiment 1. A method for reducing or delaying the onset of severe ocular surface disease, comprising topically administering a fluid comprising high molecular weight hyaluronic acid to the ocular surface of the eye of a human or non-human animal subject, wherein the hyaluronic acid has an intrinsic viscosity of >2.5 m³/kg and a concentration of <0.2% w/v.

Embodiment 2. The method of embodiment 1, wherein the fluid is administered prophylactically, before the severe ocular surface disease exists.

Embodiment 3. The method of embodiment 2, wherein the subject has a mild or moderate ocular surface disease at the time of administration, and the fluid is administered prophylactically to prevent or delay the progression of the ocular surface disease to a state of severe ocular surface disease.

Embodiment 4. The method of embodiment 1, wherein the fluid is administered therapeutically, after the severe ocular surface disease exists.

Embodiment 5. The method of any one of embodiments 1 to 4, wherein the severe ocular surface disease has one or more of the following characteristics: leukocyte invasion at the ocular surface and tears, CD44 upregulation at the ocular surface, and activation of an immune cascade that includes one or more of IL-1, IL-2, IL-5, IL-6, IL-8, CXCL8, IL-10, IL-12, IL-16, IL-33, MCP1, CCL2, MIP1d (also known as CCL15), ENA-78, CXCL5, sILR1, sIL-6R, sgp sEGFR, sTNFR, I-17A, IL-21, IL-22, CXCL9, MIG, CXCL11, I-TAC, CXCL10, IP-10, MIP-10, CCL4, RANTES, and CCL5.

Embodiment 6. The method of any preceding embodiment, wherein the severe ocular surface disease is caused by an external stimulus resulting in a disruption of the smoothness and/or integrity of the ocular surface (e.g., medical therapy, ocular surgery, non-surgical trauma, contact lens wearing, infection (e.g., bacterial, viral, or fungal), allergen, hapten, toxic agent, or irritative substance).

Embodiment 7. The method of any one of embodiments 1 to 5, wherein the severe ocular surface disease is caused by an internal stimulus (e.g., hormonal disturbance, rheumatic disease, epithelial-mesenchymal transition (EMT), or autoimmune disease).

Embodiment 8. The method of any preceding embodiment, wherein the severe ocular surface disease is caused by a wound of the eye.

Embodiment 9. The method of embodiment 8, wherein the wound is caused by physical trauma, chemical trauma, or radiation (radiation injury).

Embodiment 10. The method of embodiment 8, wherein the wound is caused by an ocular surgery.

Embodiment 11. The method of embodiment 10, wherein the ocular surgery is selected from among natural or artificial corneal transplantation, corneal implantation (e.g., intracorneal rings (ICRs), and keratoprosthesis), glaucoma surgery, cataract surgery (e.g., phacoemulsification, extracapsular cataract surgery, or intracapsular surgery), refractive surgery (e.g., radial keratotomy or refractive corneal incision), retinal surgery, squint (strabismus) surgery, corrective laser eye surgery (e.g., laser-assisted in situ keratomileusis (LASIK) or photorefractive keratectomy (PRK)), and cross-linking surgery.

Embodiment 12. The method of any preceding embodiment, wherein the severe ocular surface disease is accompanied by acute ocular surface inflammation.

Embodiment 13. The method of any one of embodiments 1 to 11, wherein the severe ocular surface disease is accompanied by chronic ocular surface inflammation.

Embodiment 14. The method of any one of embodiments 1 to 6, wherein the severe ocular surface disease is eye allergy.

Embodiment 15. The method of any one of embodiments 1 to 6, wherein the severe ocular surface disease is severe allergic keratoconjunctivitis (e.g., seasonal or non-seasonal) that causes one or more of the following in the subject: ocular pain; visual disturbance; acute loss of tissue homeostasis from a dysfunction of normal regulatory mechanism that maintain cellular hydration in tissues around the eye; and release of IgE and histamine.

Embodiment 16. The method of any preceding embodiment, wherein the severe ocular surface disease comprises a corneal opacity.

Embodiment 17. The method of embodiment 16, wherein the corneal opacity is caused by corneal edema.

Embodiment 18. The method of embodiment 17, wherein the corneal edema is caused by trauma; glaucoma; birth injury; or Fuchs dystrophy.

Embodiment 19. The method of embodiment 17, wherein the corneal opacity is caused by corneal scarring.

Embodiment 20. The method of embodiment 16, wherein the corneal opacity comprises a congenital corneal opacity.

Embodiment 21. The method of embodiment 16 or 20, wherein the corneal opacity is caused by birth trauma; Peters anomaly; dermoid tumor (limbal dermoid); sclerocornea; congenital hereditary endothelial dystrophy (CHED); mucopolysaccharidoses; congenital glaucoma, microphthalmia, metabolic disease infection; or inflammation.

Embodiment 22. The method of embodiment 16, wherein the corneal opacity is caused by a lack of blinking (e.g., due to a birth defect in a child without blink reflex).

Embodiment 23. The method of embodiment 16, wherein the subject has Stevens-Johnson Syndrome.

Embodiment 24. The method of embodiment 16, wherein the corneal opacity is caused by inflammation; sensitivity to non-infectious bacteria; ulcer on the eye; infection; keratitis; trachoma; onchocerciasis (River blindness); dryness of the eye due to Sjogren syndrome, vitamin A deficiency, LASIK, LASEK, or PRK eye surgery; dystrophy (inherited metabolic disease); keratoconus; or injury to the eye (e.g., chemical burn, welding injury, etc.).

Embodiment 25. The method of embodiment 16, wherein the fluid is administered in combination with another intervention for treatment of the corneal opacity or treatment of the cause of the opacity (e.g., antibiotic, steroid, phototherapeutic keratectomy (PTK), cornea transplant).

Embodiment 26. The method of any preceding embodiment, wherein the severe ocular surface disease causes discomfort such as itchiness or ocular pain.

Embodiment 27. The method of any preceding embodiment, wherein the subject is immunocompromised.

Embodiment 28. The method of any preceding embodiment, wherein the fluid contains no other bioactive agent (e.g., no hydrophobic active ingredient).

Embodiment 29. The method of any preceding embodiment, wherein the fluid contains no other immunomodulatory agent, immunosuppressive agent, or antibiotic.

Embodiment 30. The method of any one of embodiments 1 to 27, wherein the fluid further comprises a bioactive agent (e.g., a hydrophobic active ingredient).

Embodiment 31. The method of embodiment 30, wherein the bioactive agent is irritative or damaging to the eye.

Embodiment 32. The method of any one of embodiments 1 to 27, 29, 30, or 31 wherein the fluid further comprises a preservative or detergent.

Embodiment 33. The method of embodiment 32, wherein the preservative or detergent is irritative or damaging to the eye.

Embodiment 34. The method of any preceding embodiment, wherein the fluid is administered before, during, and/or after administration of a composition comprising: a bioactive agent (e.g., a hydrophobic active ingredient), preservative, detergent, or combination of two or more of the foregoing.

Embodiment 35. The method of embodiment 34, wherein the bioactive agent, preservative, detergent, or combination is irritative or damaging to the eye.

Embodiment 36. The method of any one of embodiments 32 to 35, wherein the preservative or detergent is a chemical preservative or oxidative preservative.

Embodiment 37. The method of any one of embodiments 32 to 36, wherein the preservative or detergent is one that kills susceptible microbial cells by disrupting the lipid structure of the microbial cell membrane, thereby increasing microbial cell membrane permeability.

Embodiment 38. The method of any one of embodiments 32 to 37, wherein the preservative or detergent is one that causes damage to the corneal tissues.

Embodiment 39. The method of any one of embodiments 32 to 38, wherein the preservative or detergent is selected from the group consisting of quaternary ammonium preservative (e.g., benzalkonium chloride (BAK)), chlorobutanol, edetate disodium (EDTA), polyquaternarium-1 (e.g., Polyquad™ preservative), stabilized oxidizing agent (e.g., stabilized oxychloro complex (e.g., Purite™ preservative)), ionic-buffered preservative (e.g., sofZia™ preservative), polyhexamethylene biguanide (PHMB), sodium perborate (e.g., GenAqua™ preservative), and sorbate.

Embodiment 40. The method of any preceding embodiment, wherein the fluid is administered directly to the ocular surface as drops or as a wash (e.g., lavage).

Embodiment 41. The method of embodiment 40, wherein 1 to 3 drops are administered, 1 to 3 times per day.

Embodiment 42. The method of any one of embodiments 1 to 40, wherein the severe ocular surface disease is accompanied by chronic ocular surface inflammation, and wherein 1 to 3 drops are administered, 4, 5, 6, 7, 8, 9, or 10 or more times per day.

Embodiment 43. The method of any one of embodiments 1 to 39, wherein the fluid is administered indirectly to the ocular surface by a delivery agent (a fluid delivery agent) that is topically administered to the ocular surface or other part of the eye (e.g., a particle that is coated with and/or secretes the fluid on to the ocular surface).

Embodiment 44. The method of any preceding embodiment, wherein the hyaluronic acid has an intrinsic viscosity of >2.9 m³/kg.

Embodiment 45. The method of any one of embodiments 1 to 27, wherein the fluid further comprises a preservative.

Embodiment 46. The method of any one of embodiments 1 to 27, wherein the fluid does not further comprise a preservative (i.e., the fluid is preservative-free).

Embodiment 47. The method of any one of embodiments 1 to 27, wherein the fluid further comprises an additional glycosaminoglycan (GAG), an electrolyte (e.g., sodium chloride), a buffer (e.g., phosphate buffer), or a combination of two or more of the foregoing.

Embodiment 48. The method of any preceding embodiment, wherein the eye of the subject does not have aqueous tear deficiency (ATD) at the time of said administering (i.e., in the absence of ATD).

Embodiment 49. The method of any preceding embodiment, wherein the eye of the subject does not have qualitative dry eye at the time of said administering (i.e., in the absence of qualitative dry eye).

Embodiment 50. The method of any preceding embodiment, wherein the eye of the subject does not have dry eye syndrome at the time of said administering (i.e., in the absence of aqueous tear deficiency or qualitative dry eye).

Embodiment 51. The method of any preceding embodiment, wherein the subject is not suffering from an aqueous tear deficiency (ATD), and wherein the subject has an ocular surface abnormality (a topographic anomaly) comprising elevations on the cornea or elsewhere on the eye surface for which the normal tear film (tear film of normal surface tension and viscosity) does not cover, resulting in areas of friction at the ocular surface, and wherein the administered fluid reduces the friction.

Embodiment 52. The method of any preceding embodiment, wherein the eye of the subject is in homeostasis at the ocular surface at the time of said administering.

Embodiment 53. The method of any preceding embodiment, further comprising applying a bandage contact lens to the eye before, during, or after said administering.

Embodiment 54. The method of embodiment 53, wherein the fluid is administered and the bandage contact lens is applied after ocular surgery.

Embodiment 55. The method of any preceding embodiment, wherein the administered fluid increases visual performance of the eye.

Embodiment 56. The method of embodiment 54, wherein the fluid is administered before, during, and/or after ocular surgery, and wherein the subject recovers visual performance following the ocular surgery (i.e., recovers greater visual performance following the ocular surgery than the subject would have in the absence of administration of the fluid).

Embodiment 57. The method of any preceding embodiment, wherein the hyaluronic acid has a molecular weight of at least 3 million Daltons.

Embodiment 58. The method of any preceding embodiment, wherein the hyaluronic acid has a molecular weight in the range of 3 million to 4 million Daltons.

Embodiment 59. A kit comprising: the fluid of any one of embodiments 1 to 48, and one or more bandage contact lenses.

The present invention also concerns a fluid (a topical fluid) for topical administration to an ocular surface.

Embodiment 60. A fluid for use in reducing or delaying the onset of severe ocular surface disease, wherein the fluid comprises high molecular weight hyaluronic acid having an intrinsic viscosity of >2.5 m³/kg and a concentration of <0.2% w/v, and wherein the fluid is topically administered to the ocular surface of the eye of a human or non-human animal subject.

Embodiment 61. The fluid of embodiment 60, wherein the fluid is administered prophylactically, before the severe ocular surface disease exists.

Embodiment 62. The fluid of embodiment 61, wherein the subject has a mild or moderate ocular surface disease at the time of administration, and the fluid is administered prophylactically to prevent or delay the progression of the ocular surface disease to a state of severe ocular surface disease.

Embodiment 63. The fluid of embodiment 60, wherein the fluid is administered therapeutically, after the severe ocular surface disease exists.

Embodiment 64. The fluid of any one of embodiments 60 to 63, wherein the severe ocular surface disease has one or more of the following characteristics: leukocyte invasion at the ocular surface and tears, CD44 upregulation at the ocular surface, and activation of an immune cascade that includes one or more of IL-1, IL-2, IL-5, IL-6, IL-8, CXCL8, IL-10, IL-12, IL-16, IL-33, MCP1, CCL2, MIP1d (also known as CCL15), ENA-78, CXCL5, sILR1, sIL-6R, sgp sEGFR, sTNFR, I-17A, IL-21, IL-22, CXCL9, MIG, CXCL11, I-TAC, CXCL10, IP-10, MIP-10, CCL4, RANTES, and CCL5.

Embodiment 65. The fluid of any one of embodiments 50 to 63, wherein the severe ocular surface disease is caused by an external stimulus resulting in a disruption of the smoothness and/or integrity of the ocular surface (e.g., medical therapy, ocular surgery, non-surgical trauma, contact lens wearing, microbial infection, allergen, hapten, toxic agent, or irritative substance).

Embodiment 66. The fluid of any one of embodiments 60 to 64, wherein the severe ocular surface disease is caused by an internal stimulus (e.g., hormonal disturbance, rheumatic disease, epithelial-mesenchymal transition (EMT), or autoimmune disease).

Embodiment 67. The fluid of any one of embodiments 60 to 65, wherein the severe ocular surface disease is caused by a wound of the eye.

Embodiment 68. The fluid of embodiment 67, wherein the wound is caused by physical trauma, chemical trauma, or radiation (radiation injury).

Embodiment 69. The fluid of embodiment 67, wherein the wound is caused by an ocular surgery.

Embodiment 70. The fluid of embodiment 69, wherein the ocular surgery is selected from among natural or artificial corneal transplantation, corneal implantation (e.g., intracorneal rings (ICRs), and keratoprosthesis), glaucoma surgery, cataract surgery (e.g., phacoemulsification, extracapsular cataract surgery, or intracapsular surgery), refractive surgery (e.g., radial keratotomy or refractive corneal incision), retinal surgery, squint (strabismus) surgery, corrective laser eye surgery (e.g., laser-assisted in situ keratomileusis (LASIK) or photorefractive keratectomy (PRK)), and cross-linking surgery.

Embodiment 71. The fluid of any one of embodiments 60 to 63, wherein the severe ocular surface disease is acute ocular surface inflammation.

Embodiment 72. The fluid of any one of embodiments 60 to 70, wherein the severe ocular surface disease is accompanied by chronic ocular surface inflammation.

Embodiment 73. The fluid of any one of embodiments 60 to 52, wherein the severe ocular surface disease is eye allergy.

Embodiment 74. The fluid of any one of embodiments 60 to 64, wherein the severe ocular surface disease is severe allergic keratoconjunctivitis (e.g., seasonal or non-seasonal) that causes one or more of the following in the subject: ocular pain; visual disturbance; acute loss of tissue homeostasis from a dysfunction of normal regulatory mechanism that maintain cellular hydration in tissues around the eye; and release of IgE and histamine.

Embodiment 75. The fluid of any one of embodiments 60 to 65, wherein the severe ocular surface disease causes discomfort such as itchiness or ocular pain.

Embodiment 76. The fluid of any one of embodiments 60 to 65, wherein the fluid is administered prophylactically, to prevent or delay the onset of the severe ocular surface disease, and wherein the subject is immunocompromised.

Embodiment 77. The fluid of any one of embodiments 60 to 66, wherein the fluid contains no other bioactive agent (e.g., no hydrophobic active ingredient).

Embodiment 78. The fluid of any one of embodiments 60 to 67, wherein the fluid contains no other immunomodulatory agent, immunosuppressive agent, or antibiotic.

Embodiments 79. The fluid of any one of embodiments 60 to 68, wherein the fluid further comprises a bioactive agent (e.g., a hydrophobic active ingredient).

Embodiment 80. The fluid of embodiment 79, wherein the bioactive agent is irritative or damaging to the eye.

Embodiment 81. The fluid of any one of embodiments 60 to 64, wherein the fluid further comprises a preservative or detergent.

Embodiment 82. The fluid of embodiment 81, wherein the preservative or detergent is irritative or damaging to the eye.

Embodiment 83. The fluid of any one of embodiments 60 to 64, wherein the fluid is administered before, during, and/or after administration of a composition comprising: a bioactive agent (e.g., a hydrophobic active ingredient), preservative, detergent, or combination of two or more of the foregoing.

Embodiment 84. The fluid of embodiment 83, wherein the bioactive agent, preservative, detergent, or combination is irritative or damaging to the eye.

Embodiment 85. The fluid of embodiment 81, wherein the preservative or detergent is a chemical preservative or oxidative preservative.

Embodiment 86. The fluid of embodiment 81, wherein the preservative or detergent is one that kills susceptible microbial cells by disrupting the lipid structure of the microbial cell membrane, thereby increasing microbial cell membrane permeability.

Embodiment 87. The fluid of embodiment 81, wherein the preservative or detergent is one that causes damage to the corneal tissues.

Embodiment 88. The fluid of embodiment 82, wherein the preservative or detergent is selected from the group consisting of quaternary ammonium preservative (e.g., benzalkonium chloride (BAK)), chlorobutanol, edetate disodium (EDTA), polyquaternarium-1 (e.g., Polyquad™ preservative), stabilized oxidizing agent (e.g., stabilized oxychloro complex (e.g., Purite™ preservative)), ionic-buffered preservative (e.g., sofZia™ preservative), polyhexamethylene biguanide (PHMB), sodium perborate (e.g., GenAqua™ preservative), and sorbate.

Embodiment 89. The fluid of any one of embodiments 60 to 63, wherein the fluid is administered directly to the ocular surface as drops or as a wash.

Embodiment 90. The fluid of embodiment 89, wherein 1 to 3 drops are administered, 1 to 3 times per day.

Embodiment 91. The fluid of any one of embodiments 60 to 63, wherein the inflammation is chronic ocular surface inflammation, and wherein 1 to 3 drops are administered 4, 5, 6, 7, 8, 9, or 10 or more times per day.

Embodiment 92. The fluid of any one of embodiments 60 to 63, wherein the fluid is administered indirectly to the ocular surface by a delivery agent that is topically administered to the ocular surface or other part of the eye (e.g., a particle that is coated with and/or secretes the fluid on to the ocular surface).

Embodiment 93. The fluid of any one of embodiments 60 to 63, wherein the hyaluronic acid has an intrinsic viscosity of >2.9 m³/kg.

Embodiment 94. The fluid of any one of embodiments 60 to 63, wherein the fluid further comprises a preservative.

Embodiment 95. The fluid of any one of embodiments 60 to 63, wherein the fluid does not further comprise a preservative (i.e., the fluid is preservative-free).

Embodiment 96. The fluid of any one of embodiments 60 to 63, wherein the fluid further comprises an additional glycosaminoglycan (GAG), an electrolyte (e.g., sodium chloride), a buffer (e.g., phosphate buffer), or a combination of two or more of the foregoing.

Embodiment 97. The fluid of any one of embodiments 60 to 63, wherein the eye of the subject does not have aqueous tear deficiency (ATD) at the time of said administering (i.e., in the absence of ATD).

Embodiment 98. The fluid of any one of embodiments 60 to 63, wherein the eye of the subject does not have qualitative dry eye at the time of said administering (i.e., in the absence of qualitative dry eye).

Embodiment 99. The fluid of any one of embodiments 60 to 63, wherein the eye of the subject does not have dry eye syndrome at the time of said administering (i.e., in the absence of aqueous tear deficiency or qualitative dry eye).

Embodiment 100. The fluid of any one of embodiments 60 to 63, wherein the subject is not suffering from an aqueous tear deficiency (ATD), and wherein the subject has an ocular surface abnormality (a topographic anomaly) comprising elevations on the cornea or elsewhere on the eye surface for which the normal tear film (tear film of normal surface tension and viscosity) does not cover, resulting in areas of friction at the ocular surface, and wherein the administered fluid reduces the friction.

Embodiment 101. The fluid of any of embodiments 60 to 63, wherein the eye of the subject is in homeostasis at the ocular surface at the time of said administering.

Embodiment 102. The fluid of any one of embodiments 60 to 63, further comprising applying a bandage contact lens to the eye before, during, or after said administering.

Embodiment 103. The fluid of embodiment 102, wherein the fluid is administered and the bandage contact lens is applied after ocular surgery.

Embodiment 104. The fluid of any one of embodiments 60 to 63, wherein the administered fluid increases visual performance of the eye.

Embodiment 105. The fluid of embodiment 104, wherein the fluid is administered before, during, and/or after ocular surgery, and wherein the subject recovers visual performance following the ocular surgery.

Embodiment 106. The fluid of any one of embodiments 60 to 105, wherein the hyaluronic acid has a molecular weight of at least 3 million Daltons.

Embodiment 107. The fluid of any one of embodiments 60 to 106, wherein the hyaluronic acid has a molecular weight in the range of 3 million to 4 million Daltons.

Definitions

The term “a,” “an,” “the” and similar terms used in the context of the present invention (especially in the context of the claims) are to be construed to cover both the singular and plural unless otherwise indicated herein or clearly contradicted by the context. Thus, for example, reference “a cell” or “a compound” should be construed to cover both a singular cell or singular compound and a plurality of cells and a plurality of compounds unless indicated otherwise or clearly contradicted by the context. Similarly, the word “or” is intended to include “and” unless the context clearly indicates otherwise. The abbreviation, “e.g.” is derived from the Latin exempli gratia, and is used herein to indicate a non-limiting example. Thus, the abbreviation “e.g.” is synonymous with the term “for example.”

The term “effective amount” in the context of the administered fluid of the invention means the amount of fluid necessary to obtain a desired result, such as reduction or elimination of the severe ocular surface disease, or reduction or elimination of a mild or moderate ocular surface disease and prevention or delayed progression to a severe ocular surface disease. In some embodiments, an effective amount may be the amount capable of preventing, delaying the onset of, treating, or ameliorating a severe ocular surface disease, or otherwise capable of producing an intended therapeutic effect.

The term “isolated,” when used as a modifier of a composition, means that the compositions are made by human intervention or are separated from their naturally occurring in vivo environment. Generally, compositions so separated are substantially free of one or more materials with which they normally associate with in nature, for example, one or more protein, nucleic acid, lipid, carbohydrate, cell membrane. A “substantially pure” molecule can be combined with one or more other molecules. Thus, the term “substantially pure” does not exclude combinations of compositions. Substantial purity can be at least about 60% or more of the molecule by mass. Purity can also be about 70% or 80% or more, and can be greater, for example, 90% or more. Purity can be determined by any appropriate method, including, for example, UV spectroscopy, chromatography (e.g., HPLC, gas phase), gel electrophoresis (e.g., silver or coomassie staining) and sequence analysis (for nucleic acid and peptide).

As used herein, the term “homeostasis” refers to the capacity of a physiological system to maintain internal stability, or to the state of stability itself, owing to the coordinated response of its parts to any situation or stimulus that would tend to disturb its normal, non-pathological condition or function.

As used herein, the term “hyaluronic acid” (HA) refers to the glycosaminoglycan composed of disaccharide repeats of N-acetylglucosamine and glucuronic acid found in nature, also known as hyaluronan (e.g., the straight chain, glycosaminoglycan polymer composed of repeating units of the disaccharide [-D-glucuronic acid-b1,3-N-acetyl-D-glucosamine-b1,4-]n), as well as derivatives of hyaluronan having chemical modifications such as esters of hyaluronan, amide derivatives, alkyl-amine derivatives, low molecular weight and high molecular weight forms of hyaluronans, and cross-linked forms such as hylans. Thus, the disaccharide chain may be linear or non-linear. Hyaluronan can be cross-linked by attaching cross-linkers such as thiols, methacrylates, hexadecylamides, and tyramines. Hyaluronan can also be cross-linked directly with formaldehyde and divinylsulfone. Examples of hylans include, but are not limited to, hylan A, hylan A (a formaldehyde cross-linked glycosaminoglycan polymer), hylan B (a divinylsulfone cross-linked glycosaminoglycan polymer), and hylan G-F 20 (Cowman M K et al., Carbohydrate Polymers 2000, 41:229-235; Takigami S et al., Carbohydrate Polymers, 1993, 22:153-160; Balazs E A et al., “Hyaluronan, its cross-linked derivative—Hylan—and their medical applications”, in Cellulosics Utilization: Research and Rewards in Cellulosics, Proceedings of Nisshinbo International Conference on Cellulosics Utilization in the Near Future (Eds Inagaki, H and Phillips G O), Elsevier Applied Science (1989), NY, pp. 233-241; Koehler L et al., Scientific Reports, 2017, 7, article no. 1210; and Pavan M et al., Carbohydr Polym, 2013, 97(2): 321-326; which are each incorporated herein by reference in their entirety).

The term “hyaluronic acid” or HA includes HA itself and pharmaceutically acceptable salts thereof. The HA can be formulated into pharmaceutically-acceptable salt forms. Pharmaceutically-acceptable salts of HA can be prepared using conventional techniques.

The term “high molecular weight” or “HMW” in the context of hyaluronic acid of the invention refers to hyaluronic acid having an intrinsic viscosity of >2.5 m³/kg as determined by the method of the European Pharmacopoeia 9.0, “Sodium Hyaluronate”, page 3584 (which is incorporated herein by reference in its entirety). Briefly, the intrinsic viscosity [η] is calculated by linear least-squares regression analysis using the Martin equation: Log₁₀(n_r−1/c)=log₁₀[η]+κ[η]c. In some embodiments, the high molecular weight hyaluronic acid has an intrinsic viscosity of greater than 2.9 m³/kg.

As used herein, the term “immunocompromised” refers to a subject with an innate, acquired, or induced inability to develop a normal immune response. An immunocompromised subject, therefore, has a weakened or impaired immune system relative to one of a normal subject. A subject with a weakened or impaired immune system has an “immunodeficiency” or “immunocompromised condition,” which is associated with a primary or secondary deficiency, induced or non-induced, in one or more of the elements of the normal immune defense system. An immunocompromised condition may be due to a medical treatment, e.g., radiation therapy, chemotherapy or other immunosuppressing treatment, such as induced by treatment with steroids, cyclophosphamide, azathioprine, methotrexate, cyclosporine or rapamycin, in particular in relation to cancer treatment or the treatment or prevention of transplant rejection. The presence of an immunocompromised condition in a subject can be diagnosed by any suitable technique known to persons of skill the art. Strong indicators that an immunocompromised condition may be present is when rare diseases occur or the subject gets ill from organisms that do not normally cause diseases, especially if the subject gets repeatedly infected. Other possibilities are typically considered, such as recently acquired infections, for example, HIV, hepatitis, tuberculosis, etc. Generally, however, definitive diagnoses are based on laboratory tests that determine the exact nature of the immunocompromised condition. Most tests are performed on blood samples. Blood contains antibodies, lymphocytes, phagocytes, and complement components, all of the major immune components that might cause immunodeficiency. A blood cell count can be used to determine if the number of phagocytic cells or lymphocytes is below normal. Lower than normal counts of either of these two cell types correlates with an immunocompromised condition. The blood cells are also typically checked for their appearance. Sometimes, a subject may have normal cell counts, but the cells are structurally defective. If the lymphocyte cell count is low, further testing is usually conducted to determine whether any particular type of lymphocyte is lower than normal. A lymphocyte proliferation test may be conducted to determine if the lymphocytes can respond to stimuli. The failure to respond to stimulants correlates with an immunocompromised condition. Antibody levels and complement levels can also be determined for diagnosing the presence of an immunocompromised condition.

As used herein, the term “ocular surface” refers to the cornea and conjunctiva, and portions thereof, including the conjunctiva covering the upper and lower lids. The fluid may be topically administered to one or more parts of the ocular surface, including, for example, the entire ocular surface.

“Pharmaceutically acceptable salt” includes both acid and base addition salts. A pharmaceutically acceptable salt of HA or any one of the other compounds described herein is intended to encompass any and all pharmaceutically suitable salt forms. Preferred pharmaceutically acceptable salts described herein are pharmaceutically acceptable acid addition salts and pharmaceutically acceptable base addition salts.