In one embodiment, the methylase comprises a type II DNA methylase. In another embodiment, the methylase comprises a type I DNA methylase. In one embodiment, the methylase comprises a single domain methylase without restriction enzyme activity, or a modified methylase having a non-functional restriction enzyme activity. For example, the modification may comprise modifying the N6 position of methyladenine. In one embodiment, the DNA methylase recognition sequence comprises at least 4 base pairs. The DNA methylase may be active, such as optimally active, at about 37° C.

In one embodiment, the opposing non-protectable restriction enzyme recognition sequence is not capable of being blocked by methylation with the methylase that recognises the DNA methylase recognition sequence of the methylation-protectable restriction element. In one embodiment, the methylase that recognises the DNA methylase recognition sequence of the methylation-protectable restriction element may not recognise a sequence within or overlapping with the opposing non-protectable restriction enzyme recognition sequence. For example, the non-protectable opposing restriction enzyme recognition sequence may not overlap with or comprise a sequence that is recognised by the methylase that recognises DNA methylase recognition sequence of the methylation-protectable restriction element. The restriction enzyme recognition sequence of the methylation-protectable restriction element may be methylated, for example by the methylase that recognises the DNA methylase recognition sequence of the methylation-protectable restriction element. The methylase that recognises the DNA methylase recognition sequence of the methylation-protectable restriction element may be arranged to methylate a nucleotide within the sequence of the restriction enzyme recognition sequence of the methylation-protectable restriction element. The methylation may be capable of blocking the cutting of the DNA by the restriction enzyme that recognises the restriction enzyme recognition sequence of the methylation-protectable restriction element. In one embodiment, the restriction enzyme recognition sequence of the methylation-protectable restriction element may become non-functional as a result of methylation of at least one of the nucleotides in the sequence. In one embodiment, the methylated nucleotide may be an adenine, or the nucleotide arranged to be methylated may be an adenine. In another embodiment, the methylated nucleotide may be a cytosine, or the nucleotide arranged to be methylated may be a cytosine. The methylation may be any one of methylation types selected from N4-methylcytosine (m4C), C5-methyylcytosine (m5C) or N6-methyladenine (m6A). The skilled person will recognize that the type of methylation provided should block/impair the overlapping restriction site function. Additionally, for in vivo methylation, the strain used to express the methylase may be deficient for modification-dependent restriction enzymes that may recognize the methylated bases, such as Mcr/Mrr family restriction enzymes. In one embodiment, the methylation type may comprise N6-methyladenine (m6A).

In one embodiment, the methylase may or may not methylate the outer-most bases of the restriction enzyme recognition sequence. For example in a restriction enzyme recognition sequence of 6 bp, the bases 1 or 6 may not be methylated. In one embodiment, the methylase may or may not methylate the 2^ndbase of the restriction enzyme recognition sequence. In one embodiment, the methylase may methylate position 3, 4, or 5 for 6 bp restriction enzyme recognition sites. In one embodiment, the methylase may methylate position 3, 4, 5, or 6 for restriction enzyme recognition sites.

In an embodiment comprising two methylation-protectable restriction elements, the DNA methylase recognition sequences of each methylation-protectable restriction element may be recognised by the same methylase. The DNA methylase recognition sequence of the methylation-protectable restriction elements may comprise or consist of the same sequence.

In an embodiment comprising two methylation-protectable restriction elements, the restriction enzyme recognition sequences of each methylation-protectable restriction element may be recognised by the same restriction enzyme species. Each restriction enzyme recognition sequence of the methylation-protectable restriction elements may comprise or consist of the same sequence. The two methylation-protectable restriction elements may comprise or consist of the same sequence.

The use of the same restriction enzyme recognition sequence advantageously provides that a single restriction enzyme species can be used in a DNA assembly reaction. The use of a single restriction enzyme species opens the possibility of less complex DNA assembly, because the presence of further restriction enzyme species in a reaction significantly increases the probability the more that the sequence will randomly/inadvertently contain a recognition and restriction cut site, causing a failure in the cloning procedure.

In one embodiment, the methylation-protectable restriction element comprises or consists of a sequence according to any one of the overlapping methylation/restriction sites identified in Table 3 herein. In an alternative embodiment, the methylation-protectable restriction element comprises or consists of a sequence according to any one of the overlapping methylation/restriction sites identified in Table 3 herein, excluding those which provide methylation of the first or last base in the restriction sequence. In one embodiment, the methylation-protectable restriction element comprises or consists of the sequence GACNNGGTCTCNNNNN (BsaI/M.Osp807II—SEQ ID NO: 1). In another embodiment, the methylation-protectable restriction element comprises or consists of the sequence GAAGACGCNNNNNN (BpiI/M2.NmeMC58II—SEQ ID NO: 2). In another embodiment, the methylation-protectable restriction element comprises or consists of the sequence GAAGCTCTTCNNNN (LguI/M.XmnI—SEQ ID NO: 3).

In one embodiment, the methylation-protectable and/or non-protectable restriction enzyme recognition sequence comprises or consists of a sequence according to any one of the restriction enzyme recognition sequences identified in Table 3 herein. In an alternative embodiment, the methylation-protectable and/or non-protectable restriction enzyme recognition sequence comprises or consists of a sequence according to any one of the restriction enzyme recognition sequences identified in Table 3 herein, excluding those which provide for methylation of the first or last base in the restriction sequence. In one embodiment, the methylation-protectable and/or non-protectable restriction enzyme recognition sequence comprises or consists of the sequence GGTCTC (BsaI-SEQ ID NO: 4). In another embodiment, the methylation-protectable and/or non-protectable restriction enzyme recognition sequence comprises or consists of a sequence GAAGAC (BpiI—SEQ ID NO: 5). In another embodiment, the methylation-protectable and/or non-protectable restriction enzyme recognition sequence comprises or consists of a sequence GCTCTTC (LguI—SEQ ID NO: 6).

In one embodiment, the DNA methylase recognition sequence comprises or consists of a sequence according to any one of the type II DNA methylase recognition sequences identified in Table 3 herein. In an alternative embodiment, the DNA methylase recognition sequence comprises or consists of a sequence according to any one of the type II DNA methylase recognition sequences identified in Table 3 herein, excluding those which provide methylation of the first or last base in the restriction sequence. In one embodiment, the DNA methylase recognition sequence comprises or consists of the sequence GACNNNGTC (M.Osp807II—SEQ ID NO: 7). In another embodiment, the DNA methylase recognition sequence comprises or consists of the sequence GACGC (M2.NmeMC58II—SEQ ID NO: 8). In another embodiment, the DNA methylase recognition sequence comprises or consists of the sequence GAANNNNTTC (M.XmnI—SEQ ID NO: 9).

The restriction enzyme that recognizes the restriction enzyme recognition sequence of the methylation-protectable restriction element, and/or the opposing restriction enzyme recognition sequence, may be capable of cutting nucleic acid to leave at least a 2 bp overhang/sticky end. Alternatively, the restriction enzyme that recognizes the restriction enzyme recognition sequence of the methylation-protectable restriction element, and/or the opposing restriction enzyme recognition sequence, may be capable of cutting nucleic acid to leave at least a 3 bp overhang/sticky end. In another embodiment, the restriction enzyme that recognizes the restriction enzyme recognition sequence of the methylation-protectable restriction element, and/or the opposing restriction enzyme recognition sequence, may be capable of cutting nucleic acid to leave a 3 bp overhang/sticky end. In another embodiment, the restriction enzyme that recognizes the restriction enzyme recognition sequence of the methylation-protectable restriction element, and/or the opposing restriction enzyme recognition sequence, may be capable of cutting nucleic acid to leave a 4 bp overhang/sticky end. In another embodiment, the restriction enzyme that recognizes the restriction enzyme recognition sequence of the methylation-protectable restriction element, and/or the opposing restriction enzyme recognition sequence, may be capable of cutting nucleic acid to leave a 2 bp to 6 bp overhang/sticky end. In another embodiment, the restriction enzyme that recognizes the restriction enzyme recognition sequence of the methylation-protectable restriction element, and/or the opposing restriction enzyme recognition sequence, may be capable of cutting nucleic acid to leave a 2 bp to 5 bp overhang/sticky end. In another embodiment, the restriction enzyme that recognizes the restriction enzyme recognition sequence of the methylation-protectable restriction element, and/or the opposing restriction enzyme recognition sequence, may be capable of cutting nucleic acid to leave a 3 bp to 5 bp overhang/sticky end. In another embodiment, the restriction enzyme that recognizes the restriction enzyme recognition sequence of the methylation-protectable restriction element, and/or the opposing restriction enzyme recognition sequence, may be capable of cutting nucleic acid to leave a 4 bp to 5 bp overhang/sticky end.

In one embodiment, the restriction enzyme comprises or consists of any one type IIS restriction enzyme identified in Table 3 herein. In another embodiment, the type IIS restriction enzyme comprises or consists of BsaI, BpiI, or LguI.

In one embodiment, the DNA methylase comprises or consists of any one type II DNA methylase identified in Table 3 herein. In an alternative embodiment, the DNA methylase comprises or consists of any one type II DNA methylase identified in Table 3 herein, excluding those which provide methylation of the first or last base in the restriction sequence. In another embodiment, the DNA methylase comprises or consists of M.Osp807II, M2. NmeMC58II, or M.XmnI.

In one embodiment, the restriction enzyme and the DNA methylase comprise or consist of any one pairing of type IIS restriction enzymes and the type II DNA methylases identified in Table 3 herein. In one embodiment, the restriction enzyme and the DNA methylase comprise or consist of the pairings BsaI/M.Osp807II, BpiI/M2.NmeMC58II, or LguI/M.XmnI.

In one embodiment, the restriction enzyme recognition sequence and the DNA methylase recognition sequence comprise or consist of any one pairing of type IIS restriction enzyme recognition sequences and the type II DNA methylase recognition sequences identified in Table 3 herein. In an alternative embodiment, the restriction enzyme recognition sequence and the DNA methylase recognition sequence comprise or consist of any one pairing of type IIS restriction enzyme recognition sequences and the type II DNA methylase recognition sequences identified in Table 3 herein, excluding those which provide methylation of the first or last base in the restriction sequence.

In an embodiment comprising two methylation-protectable restriction elements (for example with a discard sequence therebetween), the overhang/sticky end produced by cutting with the restriction enzyme at the first methylation-protectable restriction element may be different in sequence than the overhang/sticky end produced by cutting with the restriction enzyme at the second methylation-protectable restriction element. The skilled person will be familiar with providing different overhangs for directional DNA assembly/cloning or controlling which end of the nucleic acid is ligated to an insert/fragment of interest.

In one embodiment involving a maintained composite element, the nucleic acid may comprise or consist of the sequence of GACNNGGTCTCNNNNNNGAGACC (SEQ ID NO: 10). In another embodiment involving a maintained composite element, the nucleic acid may comprise or consist of the sequence of GAAGACGCNNNNNNGTCTTC (SEQ ID NO: 11). In another embodiment involving a maintained composite element, the nucleic acid may comprise or consist of the sequence of GAAGCTCTTCNNNNNGAAGAGC (SEQ ID NO: 12). N may mean A, C, T or G. Other sequences may be provided according to the combined/overlapping restriction enzyme and methylase recognition sequences provided in table 3, wherein following the series of N bases, the sequence further comprises the palindromic/reverse-complementary sequence of the restriction enzyme recognition sequence.

In one embodiment involving two maintained composite elements, the nucleic acid may comprise or consist of the sequence of GACNNGGTCTCNNNNNNGAGACC(N^x)GGTCTCNNNNNNGAGACCNNGTC (SEQ ID NO: 13). In another embodiment involving two maintained composite elements, the nucleic acid may comprise or consist of the sequence of GAAGACGCNNNNNNGTCTTC(N^x)GAAGACNNNNNNGCGTCTTC (SEQ ID NO: 14). In another embodiment involving two maintained composite elements, the nucleic acid consist of may comprise or the sequence of GAAGCTCTTCNNNNNGAAGAGC(N^x)GCTCTTCNNNNNGAAGAGCTTC (SEQ ID NO: 15). N may mean A, C, T or G. (N^x) may mean any number of A, C, T or G, or between 0 bp and 300 kbp of A, C, T, or G.

The skilled person will understand that for some restriction enzyme recognition sequences, such as for LguI, the number of nucleotides (N^x) between two maintained composite elements could be negative with the two inner nonprotectable restriction enzyme recognition sites overlapping. Therefore, in another embodiment involving two maintained composite elements, the nucleic acid may comprise or consist of the sequence of GAAGCTCTTCNNNNNGAAGAGCTCTTCNNNNNGAAGAGCTTC (SEQ ID NO: 16) (the overlapping nucleotides are shown in bold and underlined).

In one embodiment involving a truncated composite element, the nucleic acid may comprise or consist of the sequence of GACNNGGTCTCNNGAGACC (SEQ ID NO: 17). In one embodiment involving a truncated composite element, the nucleic acid may comprise or consist of the sequence of GAAGCTCTTCNNGAAGAGC (SEQ ID NO: 18). N may mean A, C, T or G. N may mean A, C, T or G. Other sequences may be provided according to the combined/overlapping restriction enzyme and methylase recognition sequences provided in table 3, wherein following the series of N bases, the sequence further comprises the palindromic/reverse-complementary sequence of the restriction enzyme recognition sequence, and wherein the number of N bases between the restriction recognition sequences is reduced by the number overhang bases produced by the selected restriction enzyme.

In one embodiment involving a maintained and truncated composite element, the nucleic acid may comprise or consist of the sequence of GACNNGGTCTCNNNNNNGAGACC(N^x)GGTCTCNNGAGACCNNGTC (SEQ ID NO: 19). In one embodiment involving a maintained and truncated composite element, the nucleic acid comprise or consist of may the sequence of GAAGCTCTTCNNNNNGAAGAGC(N^x)GCTCTTCNNGAAGAGCTTC (SEQ ID NO: 20). N may mean A, C, T or G. (N^x) may mean any number of A, C, T or G, or between 0 bp and 300 kbp of A, C, T, or G.

The skilled person will understand that for some restriction enzyme recognition sequences, such as for LguI, the number of nucleotides (N^x) between a maintained and truncated composite element could be negative with the two inner nonprotectable restriction enzyme recognition sites overlapping. Therefore, in another embodiment involving a maintained and truncated composite element, the nucleic acid may comprise or consist of the sequence of GAAGCTCTTCNNNNNGAAGAGCTCTTCNNGAAGAGCTTC (SEQ ID NO: 21) (the overlapping nucleotides are shown in bold and underlined).

In one embodiment involving a maintained and truncated composite element, the nucleic acid may comprise or consist of the sequence of GACNNGGTCTCNNGAGACC(N^x)GGTCTCNNNNNNGAGACCNNGTC (SEQ ID NO: 22). In one embodiment involving a maintained and truncated composite element, the nucleic acid may comprise or consist of the sequence of GAAGCTCTTCNNGAAGAGC(N^x)GCTCTTCNNNNNGAAGAGCTTC (SEQ ID NO: 23). N may mean A, C, T or G. (N^x) may mean any number of A, C, T or G, or between 0 bp and 300 kbp of A, C, T, or G. In another embodiment involving a maintained and truncated composite element, the nucleic acid may comprise or consist of the sequence of GAAGCTCTTCNNGAAGAGCTCTTCNNNNNGAAGAGCTTC (SEQ ID NO: 24) (the overlapping nucleotides are shown in bold and underlined).

In one embodiment involving two truncated composite elements, the nucleic acid may comprise or consist of the sequence of GACNNGGTCTCNNGAGACC(N^x)GGTCTCNNGAGACCNNGTC (SEQ ID NO: 25). In one embodiment involving two truncated composite elements, the nucleic acid may comprise or consist of the sequence of GAAGCTCTTCNNGAAGAGC(N)GCTCTTCNNGAAGAGCTTC (SEQ ID NO: 26). N may mean A, C, T or G. (N^x) may mean any number of A, C, T or G, or between 0 bp and 300 kbp of A, C, T, or G.

The skilled person will understand that for some restriction enzyme recognition sequences, such as for LguI, the number of nucleotides (N^x) between two truncated composite elements could be negative with the two inner nonprotectable restriction enzyme recognition sites overlapping. Therefore, in another embodiment involving two truncated composite elements, the nucleic acid may comprise or consist of the sequence of GAAGCTCTTCNNGAAGAGCTCTTCNNGAAGAGCTTC (SEQ ID NO: 27) (the overlapping nucleotides are shown in bold and underlined).

The nucleic acid may be isolated nucleic acid. In one embodiment, the nucleic acid may be synthetic (i.e. not found in nature). In one embodiment, the nucleic acid may be isolated or derived from a bacterial strain, such as E. coli, that expresses a DNA methylase that recognises (and methylates) the methylase recognition sequence of the methylation-protectable restriction element. For example, a bacterial strain, such as E. coli, that expresses any one type II DNA methylase identified in Table 3 herein. In one embodiment the nucleic acid may be isolated or derived from a bacterial strain, such as E. coli, that expresses M.Osp807II, M2.NmeMC58II, or M.XmnI. The expressed DNA methylase may be functional. The bacterial strain may be genetically modified/engineered to express such DNA methylase.

The nucleic acid may be a vector, such as a cloning vector. The skilled person will be familiar with various cloning vectors, which may include, for example, a replication origin, a selectable marker, a reporter gene, expression elements, or combinations thereof. The vector may comprise nucleic acid that comprises a DNA element that supports the replication of DNA that contains it (e.g. a replication origin) and a selection marker (e.g. an antibiotic selection marker). The vector may be a high or low copy number vector. In one embodiment, the vector is a high copy number vector. The skilled person will be familiar with cloning methods and materials/vectors, for example as provided in Green, M. R., Sambrook, J. Molecular Cloning: A Laboratory Manual. 4th. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY; 2012, which is herein incorporated by reference.

In linearized embodiments of the nucleic acid, the nucleic acid may not comprise more than two copies/sites of the restriction enzyme recognition sequence.

According to another aspect of the invention, there is provided a method of assembling DNA comprising:

- providing a linearised methylated nucleic acid according to the invention, wherein the methylation-protectable restriction element is methylated with the DNA methylase;
- providing two or more DNA fragments of interest for assembly with the linearised methylated nucleic acid, wherein a first DNA fragment comprises a complementary overhang for ligation with a first end of the linearised methylated nucleic acid, and a second DNA fragment comprises complementary overhangs for ligation with the other/second end of the linearised methylated nucleic acid; and
- (i) the first and second DNA fragments further comprise complementary overhangs for ligation with each other; or
- (ii) the first and second DNA fragments further comprise complementary overhangs for ligation with one or more further DNA fragments having complementary overhangs, such that ligating the DNA fragments and the linearised methylated nucleic acid with a DNA ligase would result in a single assembled DNA molecule;
- ligating the DNA fragments and linearised methylated nucleic acid with a ligase to form a single assembled DNA molecule comprising the sequence of the assembled DNA fragments flanked by the restriction enzyme recognition sequences of the methylation-protectable restriction elements.

The single assembled DNA molecule may be circular, such as a circular DNA vector.

Advantageously, further cutting of the single assembled DNA molecule by the restriction enzyme that recognises the restriction enzyme recognition sequences of the methylation-protectable restriction elements is blocked by the methylation of the restriction enzyme recognition sequences.

The linearised nucleic acid may be provided by providing a nucleic acid according to the invention in the form of a circular destination vector comprising two methylated methylation-protectable restriction elements and a discard sequence therebetween, wherein the method comprises the step of cutting the circular destination vector with restriction enzymes that recognise the opposing non-protectable restriction enzyme recognition sequences in the discard sequence, thereby leaving a linearised nucleic acid having overhangs defined by the restriction enzymes. The restriction enzymes may be the same species. The restriction enzyme recognition sequences of the methylated methylation-protectable restriction elements may comprise the same sequence. In one embodiment, the restriction enzyme recognition sequences of the methylated methylation-protectable restriction elements and the opposing non-protectable restriction enzyme recognition sequences may comprise the same sequence.

The restriction enzyme and methylase (or recognition sequences thereof) used in the method of the invention may be selected in accordance with the restriction enzyme and methylases (or recognition sequences thereof) provided for the nucleic acid aspect of the present invention herein.

The circular destination vector may be purified/isolated from a bacterial strain that expresses the DNA methylase that recognises the DNA methylase recognition sequence of the methylation-protectable restriction element. In another embodiment, the circular or linearised destination vector may be methylated by the DNA methylase in vitro.

The two or more DNA fragments of interest for assembly with the nucleic acid may be provided in circular donation vectors, wherein the method comprises the step of cutting the circular donation vectors to release the DNA fragments of interest. The cutting of the donor vectors may be with a restriction enzyme that leaves complementary overhangs for ligation with the linearised methylated nucleic acid. The cutting of the donor vectors may be with a restriction enzyme that leaves complementary overhangs for ligation with the linearised methylated nucleic acid and with another DNA fragment of interest for insertion. The cutting of the donor vectors may be with a restriction enzyme that leaves complementary overhangs for ligation with two other DNA fragments of interest for insertion.

The restriction enzyme for cutting the circular donation vectors may comprise a type IIS restriction enzyme. The restriction enzyme for cutting the circular donation vectors may comprise the same restriction enzyme, or a restriction enzyme that recognises the same sequence, as the restriction enzyme used to cut the circular destination vector. The complementary overhangs of the DNA fragments of interest for ligation with the linearised nucleic acid may be defined by the restriction enzyme.

Advantageously, the provision of the same restriction enzyme recognition sequence for the methylation-protectable restriction elements, the opposing restriction enzyme recognition sequence on the discard sequence, and the restriction enzyme recognition sequences on the donor vectors provides that a single type of restriction enzyme can be used. This allows for reduced cost/complexity in removing internal type IIS restriction site from DNA parts, because there are less restriction sites to remove. This makes assembly of longer sequence easier and less costly. Furthermore, the invention allows for a so-called “one-pot” reaction, whereby the restriction and ligation can be carried out in one pot in a single step.

Therefore, the method may combine the steps of restricting and ligating. In particular, the circular destination vector, the donor vectors, the restriction enzyme and the ligase may be provided in the same composition.

Following assembly of the single assembled DNA molecule, the method may further comprise the step of transforming the single assembled DNA molecule into a bacterial strain that does not express the DNA methylase that recognises the DNA methylase recognition sequence of the methylation-protectable restriction element.

Advantageously, transforming the single assembled DNA molecule into a bacterial strain that does not express the DNA methylase that recognises the DNA methylase recognition sequence of the methylation-protectable restriction element results in the cloning and production of a nucleic acid sequence, such as a vector, that comprises a non-methylated (i.e. non-blocked) restriction enzyme recognition sequence to allow subsequent restriction reactions.

The method may further comprise a multistage assembly process in order to form assembled DNA fragments or vectors of greater sequence length. For example the assembled DNA, which is the product of the method of the invention, may be used further for a higher order assembly. For example, the method may comprise a second round of DNA assembly according to the invention wherein the DNA fragments of interest for assembly with the linearised methylated nucleic acid may comprise assembled DNA from a first/earlier DNA assembly round according to the invention.

Providing multiple rounds of DNA assembly to form larger fragments of DNA advantageously reduces the number of adapter/overhang sequences that are necessary. The success rate of DNA assembly is also increased with fewer DNA fragments to be assembled. Additionally, it can be easier to screen for the successfully assembled DNA with fewer fragments assembled in a single reaction.

Scarless DNA Assembly Method

According to another aspect of the present invention, there is provided a method of scarless DNA assembly of DNA fragments comprising the steps of:

- (A) providing a first linearised methylated nucleic acid according to the invention, having a maintained composite element at one end and a truncating composite element at the other end, wherein the maintained and truncating composite elements are methylated with the DNA methylase;
- providing a first DNA fragment for assembly having overhang ends that are adapted to ligate to the overhang ends of the first linearised methylated nucleic acid;
- ligating the first DNA fragment for assembly and first linearised methylated nucleic acid with a ligase to form a first methylated intermediate vector;
- transforming the first methylated intermediate vector into a bacterial strain that does not express the DNA methylase(s) that recognises either of the DNA methylase recognition sequence(s) of the maintained and truncating composite elements, thereby forming a first intermediate vector that is not methylated;
- isolating the first intermediate vector;
- (B) providing a second linearised methylated nucleic acid according to the invention, having a maintained composite element at one end and a truncating composite element at the other end, wherein the maintained and truncating composite elements are methylated with the DNA methylase, and wherein the overhang provided by the maintained composite element of the first linearised methylated nucleic acid is different in sequence to the overhang provided by the methylation-protectable restriction element of the second linearised methylated nucleic acid;
- providing a second DNA fragment for assembly having overhang ends that are adapted to ligate to the overhang ends of the second linearised methylated nucleic acid;
- ligating the second DNA fragment for assembly and second linearised methylated nucleic acid with a ligase to form a second methylated intermediate vector;
- transforming the second methylated intermediate vector into a bacterial strain that does not express the DNA methylase(s) that recognises either of the DNA methylase recognition sequence(s) of the maintained and truncating composite elements, thereby forming a second intermediate vector that is not methylated;
- isolating the second intermediate vector;
- (C) cutting the first intermediate vector with a restriction enzyme that recognises the restriction enzyme recognition sequence of the maintained composite element and a restriction enzyme that recognises the restriction enzyme recognition sequence of the truncating composite element, thereby forming a first adapted DNA fragment insert that comprises a maintained-overhang sequence that is determined by the maintained composite element and an opposing native-overhang sequence that is determined by the native sequence of the first DNA fragment for assembly;
- (D) cutting the second intermediate vector with a restriction enzyme that recognises the restriction enzyme recognition sequence of the maintained composite element and a restriction enzyme that recognises the restriction enzyme recognition sequence of the truncating composite element, thereby forming a second adapted DNA fragment insert that comprises a maintained-overhang sequence that is determined by the maintained composite element and an opposing native-overhang sequence that is determined by the native sequence of the second DNA fragment for assembly;
- wherein
- (i) the first and second adapted DNA fragments are end fragments wherein their native-overhang sequences are complementary, such that they are arranged to ligate together; or
- (ii) one or more middle DNA fragments for assembly are provided wherein the first and second adapted DNA fragments are respective end fragments in the assembly, and the one or more middle DNA fragments are arranged to be ligated between the first and second adapted DNA fragments via complementary native-overhang sequences;
- further comprising the step of ligating together, with a ligase, the first and second adapted DNA fragments, or the first and second adapted DNA fragments and one or more middle DNA fragments, to form an assembled DNA fragment having maintained-overhangs at each end.

The one or more middle DNA fragments may comprise native-overhang sequences at both ends that are determined by their native sequence. In an embodiment comprising a single middle DNA fragment for assembly, the middle DNA fragment may comprise a first native-overhang sequence that is complementary to the native-overhang of the first adapted DNA fragment and a second native-overhang sequence that is complementary to the native-overhang of the second adapted DNA fragment.

In an embodiment comprising a two or more middle DNA fragments for assembly, the middle DNA fragments may each comprise native-overhang sequences that are complementary to the native-overhang of a neighbouring middle DNA fragment, such that they can be ligated together in a pre-determined order. The first and last middle DNA fragments in a sequence will be arranged to ligate to the respective first adapted DNA fragment and second adapted DNA fragment via complementary native-overhang sequences.

In one embodiment, a middle DNA fragment for assembly may be provided by

- providing a further linearised methylated nucleic acid according to the invention, wherein the linearised methylated nucleic acid comprises a truncating composite element at each end, wherein the truncating composite elements are methylated with the DNA methylase;
- providing an adapted middle DNA fragment for assembly having overhang ends that are adapted to ligate to the overhang ends of the linearised methylated nucleic acid;
- ligating the adapted middle DNA fragment for assembly and linearised methylated nucleic acid with a ligase to form a methylated intermediate vector;
- transforming the methylated intermediate vector into a bacterial strain that does not express the DNA methylase(s) that recognises either of the DNA methylase recognition sequence(s) of the truncating composite elements, thereby forming an intermediate vector that is not methylated;
- isolating the intermediate vector;
- cutting the intermediate vector with restriction enzymes that recognise the restriction enzyme recognition sequence of the truncating composite elements, thereby forming a middle DNA fragment insert that comprises native-overhang sequences at each end that are determined by the native sequence of the middle DNA fragment for assembly.

The DNA fragments for assembly may comprise double stranded DNA comprising the overhangs/stick ends at each end. A DNA fragment for assembly may be prepared by PCR from a template, by gene synthesis, or by annealing of two oligonucleotides. Such processes, e.g. PCR or gene synthesis, may generate blunt ended double stranded DNA. For assembly starting with PCR product, an appropriate adaptor sequence containing the restriction site may be included in the PCR primer to trim the DNA to generate the overhangs for cloning into the intermediate vectors. For embodiments comprising the use of annealed oligonucleotides there are more choices. The sequence design could be the same as for PCR/gene synthesis with restriction sites at both ends for trimming with a restriction enzyme to generate the overhangs. Alternatively, two annealed oligos may form the overhangs themselves.

In one embodiment, the DNA fragments for assembly, such as the first DNA fragment for assembly, the second DNA fragment for assembly, and the one or more adapted middle DNA fragments for assembly, may be provided by PCR from a template sequence (i.e. the DNA fragments for assembly may be PCR products). The template sequence may provide the full sequence that is desired to be cloned as the assembled DNA fragment. The DNA fragments for assembly may be replicated, for example by PCR, from different sections of the template, and generated neighbouring PCR products may partially overlap in sequence (i.e. the sequence near the end of one PCR product may comprise the same sequence near the start of the next PCR product for assembly). The overlap may be partial, but sufficient in length to provide a complementary sticky end. In one embodiment, the overlap sequence within one PCR product (DNA fragment for assembly) and the overlap in the next neighbouring PCR product (DNA fragment for assembly) may be at least 2 base pairs in length. In another embodiment, the overlap sequence within one PCR product (DNA fragment for assembly) and the overlap in the next neighbouring PCR product (DNA fragment for assembly) may be at least 3 base pairs in length. In another embodiment, the overlap sequence within one PCR product (DNA fragment for assembly) and the overlap in the next neighbouring PCR product (DNA fragment for assembly) may be 3-6 base pairs in length. In another embodiment, the overlap sequence within one PCR product (DNA fragment for assembly) and the overlap in the next neighbouring PCR product (DNA fragment for assembly) may be 3-5 base pairs in length. In another embodiment, the overlap sequence within one PCR product (DNA fragment for assembly) and the overlap in the next neighbouring PCR product (DNA fragment for assembly) may be 4-5 base pairs in length. In another embodiment, the overlap sequence within one PCR product (DNA fragment for assembly) and the overlap in the next neighbouring PCR product (DNA fragment for assembly) may be 3-4 base pairs in length. In another embodiment, the overlap sequence within one PCR product (DNA fragment for assembly) and the overlap in the next neighbouring PCR product (DNA fragment for assembly) may be 4 base pairs in length.

PCR primers may be arranged to amplify the partially overlapping sections of template DNA in order to form the DNA fragments for assembly that would comprise complementary overhangs. The PCR reaction may add adaptor sequences containing the restriction recognition sequence (i.e. the universal adapter sequence, for example the restriction enzyme recognition sequence may comprise the same sequence as the restriction enzyme recognition sequence of the maintained composite element and/or truncating composite element) plus the sequence for the overhang that would be complementary to the overhang of the neighbouring/consecutive fragment for assembly. The overhang comprising the sequence that overlaps, for example by 4 bp, between consecutive/neighbouring fragments may be formed following digestion with the restriction enzyme (i.e. the overlap among PCR product is referring to the sequence, for example of 4 bp, that inside the whole adaptor sequence comprising the restriction enzyme recognition sequence).

The PCR primers may further provide the adapter sequences for the DNA fragments, which form the complementary overhang for ligation with the linearised methylated nucleic acids (such as the first linearised methylated nucleic acid, second linearised methylated nucleic acid or further linearised methylated nucleic acid) and formation of the intermediate vectors. The PCR primers may further provide a restriction enzyme recognition sequence that is arranged to direct the restriction enzyme to cut the PCR product to form the adapter sequences. The restriction enzyme recognition sequence provided by the PCR primers may be a restriction enzyme recognition sequence. The restriction enzyme recognition sequence may comprise the same sequence as the restriction enzyme recognition sequence of the maintained composite element and/or truncating composite element.

In one embodiment, the adapted middle DNA fragment comprises a region of overlapping native sequence that is the same sequence as a native overhang sequence provided in a consecutive/neighbouring DNA fragment intended for ligation. For example, where neighbouring/consecutive DNA fragments for assembly with each other are encoded from a template, they may each comprise an overlapping/same sequence region from the template, which would form the complementary native overhangs between the two neighbouring/consecutive sequences in a subsequent ligation. In one embodiment, the adapted middle DNA fragment comprises two regions of overlapping native sequence, that are arranged to form the native-overhangs of the middle DNA fragment once cut by the recognising restriction enzyme.

The method may further comprise the step of providing a linearised destination vector for insertion of the assembled DNA fragments. The linearised destination vector may comprise respective overhang sequences that are complementary to the maintained overhangs of the assembled DNA fragment. The overhang sequences of the linearised destination vector may comprise a first overhang that is complementary to the maintained-overhang sequence of the first adapted DNA fragment and a second overhang that is complementary to the second the maintained-overhang sequence of the second adapted DNA fragment.

The linearised destination vector may be provided by cutting a circular destination vector with the restriction enzyme(s) that are arranged to provide the same complementary overhangs as the assembled DNA fragments. For example, the linearised destination vector may be provided by cutting a circular destination vector with the restriction enzyme(s) that recognise the restriction enzyme recognition sequences of the maintained and/or truncating composite element. The linearised destination vector may be a cloning vector.

In one embodiment, the maintained composite element comprises the same sequence in all intermediate vectors that comprise the maintained composite element. In one embodiment, the truncated composite element comprises the same sequence in all intermediate vectors. In one embodiment, the maintained composite element comprises the same restriction enzyme recognition sequence as the truncated composite element. In one embodiment, the restriction enzyme recognition sequences of the maintained composite elements and/or the truncated composite elements of the intermediate vectors are the same. In particular the same restriction enzyme species may be used for restriction of the intermediate vectors, and optionally the destination vector.

The vectors used in the scarless assembly method of the invention may be high copy number vectors. For example the first and/or second linearised methylated nucleic acid may comprise a linearised methylated high copy number vector.

In one embodiment, the DNA assembly method of the invention is carried out in a single composition (i.e. so called “one-pot” process). For example, the DNA assembly process (including digestion with restriction enzyme and ligation) may be carried out in a single composition containing the restriction enzyme and DNA ligase using circular insert plasmids or linearized insert fragments and circular intermediate and/or destination vectors, or linearized versions thereof.

In one embodiment the methylation of the nucleic acid is carried out in a bacterial strain that is modified to expresses the methylase that recognises the methylase recognition sequence. In one embodiment the methylation of the nucleic acid is carried out in vitro.

According to another aspect of the invention, there is provided a modified bacterial strain that is modified to express a DNA methylase described herein.

The modified bacterial strain that is modified to express a DNA methylase may be an E. coli strain. The modification may comprise insertion of the methylase gene in the arsB locus of E. coli genome. The modified bacterial strain that is modified to express a DNA methylase may be formed by assembly of a vector with a methylase expression cassette followed by an antibiotic selection cassette, such as a zeocin antibiotic selection cassette, and flanked by homologous sequence from the arsb gene of E coli, followed by PCR using this as a template to generate linear DNA containing the above element, and recombineering with this fragment followed by selection with the antibiotic, such as zeocin, to generate bacteria strains with the methylase expression cassette and selection marker stably inserted into the arsB locus of E. coli genome.

According to another aspect of the invention, there is provided the use of a modified bacterial strain that is modified to express a DNA methylase described herein, for manufacturing a nucleic acid molecule according to the invention.

The modified bacterial strain may be E. coli.

According to another aspect of the inventions, there is provided a composition comprising the nucleic acid according to the invention. The composition may comprise buffer.

According to another aspect of the inventions, there is provided a kit comprising one or more nucleic acids according to the invention herein.

The kit may further comprise a restriction enzyme and/or a ligase, such as T4 DNA ligase. Additionally or alternatively, the kit may comprise a modified bacterial strain that is modified to express a DNA methylase according to the invention herein and/or a DNA methylase.

Additionally or alternatively, the kit may comprise one or more buffer solutions.

Additionally or alternatively, the kit may comprise antibiotics for clone selection.

One or more, or all, of the nucleic acid, buffer, bacterial strain, restriction enzyme, ligase, and methylase may be provided in a container, such as an Eppendorf tube.

According to another aspect of the invention, there is provided a host cell comprising nucleic acid according to the invention.

According to another aspect of the invention, there is provided the use of the nucleic acid according to the invention for assembling DNA fragments of interest, optionally wherein the use is in vitro.

Reference to the term “scarless” or “scarless assembly” herein is intended to refer to a sequence of DNA that has been assembled from multiple sequences, wherein the joint between the sequences does not comprise artefacts, such as unwanted base pairs, of the restriction and ligation of the DNA assembly process. For example, scarless assembly of DNA does not introduce additional sequence into the assembled DNA fragment. In some literatures it is also called seamless DNA assembly. In one example, an adapter sequence designed to provide complementary sticky ends to facilitate the joining of DNA fragments may be considered a “scar” between the joined sequences.

Reference to the term “adapter” herein is intended to refer to a sequence designed to provide complementary sticky ends to facilitate the joining of DNA fragments.

Reference to the term “overlap” or “overlapping” in the context of overlapping recognition sequences is intended to refer to at least part of the recognition sequence of one enzyme (for example any nucleotide other than N in a recognition sequence) being in a position of the nucleotide sequence that specifies the recognition sequence of another enzyme. (i.e. overlapping sequences share at least some common nucleotides).

Reference to the term “native” in the context of an overhang/sticky end sequence is intended to refer to the natural, non-adapted, sequence of the DNA fragment of interest to be inserted or joined to the nucleic acid. For example, the sequence will not be designed or amended relative to the sequence of the template DNA of interest. This is in contrast with overhangs determined by the vector sequence to facilitate DNA assembly, which might be introduced as a scar into the assembled sequence.

Reference to a “single assembled DNA molecule” is intended to refer to a single type of molecule, and is not intended to refer to the number of copies of the same molecule. In particular, in any given reaction or composition, there may be a plurality of copies of the single assembled DNA molecule.

Reference to “high copy number” plasmids/vectors may refer to those with a copy number of 100 or more copies per cell, including but not limiting to plasmids with replication of origins such as 1. mutated pMB1 replication origin from plasmid pUC plasmids (Vieira and Messing 1982. Gene 19:259-268; Vieira and Messing 1987. Methods Enzymol. 153:3-11; Messing 1983. Methods Enzymol. 101:20-78; Lin-Chao et al. 1992. Mol. Microbiol. 6:3385-3393, each of which are incorporated by reference herein); and 2. ColE1-derived replication origin from pBluescript plasmids—pBluescript II Phagemid Vectors, Instruction Manual, Catalog #212205, #212206. #212207 and #212208, Revision A.01, which is incorporated by reference herein). The replication origin carried by pbluescript is from pUC with a single nucleotide mutation. Particular guides on low and high copy plasmid/vector regulation can be found in Molecular Cloning A Laboratory Manual 3rd edition (2001), Joseph Sambrook and David W Russell, volume 1 page 1.4 Table 1-1 and volume 1 page 4.2 Table 4-1.

Reference to “low copy number” plasmids/vectors may refer to those with a copy number of less than 100 copies per cell, including but not limiting to plasmids with replication of origin such as 1. p15A replication origin from pACYC plasmids (Chang and Cohen 1978. J. Bacteriol. 134:1141-1156, which is incorporated by reference herein); 2. pSC101 replication origin from pSC101 plasmids (Stoker et al. 1982. Gene 18:335-341, which is incorporated by reference herein); 3. F replication origin from F plasmids (Kim et al. 1996. Genomics 34:213-218; Asakawa et al. 1997. Gene 191:69-79, each of which are incorporated by reference herein); 4. pMB1 replication origin from pBR322 plasmids (Bolivar et al. 1977. Gene 2:95-113, which is incorporated by reference herein); and 5. ColE1 replication origin from ColE1 plasmid (Kahn et al. 1979. Methods Enzymol. 68:268-280, which is incorporated by reference herein)

The skilled person will understand that optional features of one embodiment or aspect of the invention may be applicable, where appropriate, to other embodiments or aspects of the invention.

BRIEF DESCRIPTION OF THE DRAWINGS

Embodiments of the invention will now be described in more detail, by way of example only, with reference to the accompanying drawings.

FIG. 1. Identification of methylases that block overlapping methylation/restriction sites

A. Diagram of overlapping methylation/restriction sites for the initial screening. Restriction enzyme recognition sites are boxed in solid lines. The adhesive end generated by the restriction enzyme is shown by a solid line. Methylation enzyme recognition sites are boxed in dashed lines, and methylated bases are in bold font. All the methylases listed modify N6-adenine, except M.SacI and M.AspJHL3I, which modify C5-cytosine and N4-cytosine respectively.

B. Experimental designs to test blocking of overlapping methylation/restriction sites by in vivo methylation. For each methylase/restriction enzyme combination tested, the test contains plasmid a head-to-head overlapping methylation/restriction site and a nonmethylatable restriction site. Restriction digest of test plasmid prepared from normal strain would result in restriction at both sites, and releases a ˜600 bp fragment from the ˜4.3 kb vector backbone. Restriction digest of test plasmid prepared from methylase-expressing strain would result in a single ˜4.9 kb band, due blocking of overlapping methylation/restriction sites by in vivo methylation. The restriction sites of test plasmid for each restriction enzyme are shown, with the restriction site boxed in solid line, methylation sequence boxed in dashed line, and methylated bases in bold. The head-to-head arrangement of overlapping methylation/restriction site allows assaying of nickase activity in the same assay.

C. Agarose gel electrophoresis analysis of test plasmid for each overlapping methylase/restriction enzyme combination prepared in normal strain or strain expressing DNA methylase and digested with corresponding type IIS restriction enzymes. The combinations tested are BsaI with M.Osp807II (M.Osp) using test plasmid pMOP_BsaINC, BpiI with M2.NmeMC58II (M2.Nme) using test plasmid pMOP_BpiINC, and LguI with M.XmnI using test plasmid pMOP_LguINC. Test conditions are 60 fmol test plasmid digested using 5U BsaI or BpiI, or 2.5U LguI in 10 μl reaction at 37° C. for 1h. Result shows that each of the methylases tested results in successful blocking through in vivo methylation of a restriction site for the corresponding type IIS restriction enzyme when the methylase recognition site overlaps the restriction enzyme site. The data shown represents results from three independent experiments.

FIG. 2. BsaI-M.Osp807II based Metclo system

The donor plasmids contain DNA fragments to be assembled (Frag A-D) flanked by BsaI sites that generate compatible adhesive ends (labelled aaaa-eeee). The BsaI sites overlap with the M.Osp807II methylase recognition sequence. Donor plasmids were prepared from a normal strain that lacks M.Osp807II methylase activity. As a result the BsaI sites are not methylated and so the insert DNA fragments can be released by BsaI digestion. The assembly vector contains a LacZ selection marker flanked by head-to-head BsaI sites. The outside pair of BsaI sites closer to the vector backbone overlap with M.Osp807II methylation sequence, whereas the inside pair do not. Preparation of the assembly vector in M.Osp807II-expressing DH10B strain results in blocking of the outside pair of BsaI sites specifically. The LacZ fragment could still be released by BsaI through the action on the inside pair of BsaI sites. Following a one-pot reaction using BsaI and T4 DNA ligase, ligation among compatible adhesive ends results in ordered assembly of DNA fragments to the assembly vector backbone. The assembled fragment in the assembled plasmid is flanked by blocked BsaI sites refractory to BsaI digestion. Following transformation into normal strain that lacks M.Osp807II activity, methylation of the flanking restriction sites is lost, and the assembled fragment could be released by BsaI for next stage assembly.

FIG. 3. DNA assembly using Metclo vector containing functional DNA elements between the methylation-protectable restriction site and non-protectable restriction site.

A. Diagram showing assembly of a transcription unit for expression of a protein with three different domains (D1, D2, D3) using Metclo vector with the promoter element (Prom) and polyA signal (polyA) pre-embedded into the vector. Methylation-protected BsaI sites are boxed in solid line, functional BsaI sites in dotted line, and methylated bases in bold. XXXX and YYYY represents the adaptor sequence at 5′ and 3′end of the assembled DNA fragment (transcription unit) that could be released for next round of DNA assembly. They may or may not be different from the internal adaptor sequences (AATG, ACTA, ATCT and GCTT), because they are not involved in the assembly reaction.

B. Diagram showing assembly of the same transcription unit using Metclo with the promoter element and polyA signal provided in separate insert plasmids. XXXX and YYYY represents the adaptor sequence at 5′ and 3′end of the assembled DNA fragment (transcription unit) that could be released for next round of DNA assembly. They are different from the internal adaptor sequences.

FIG. 4. Principle of adhesive end switching using universal cloning vectors

A. Diagram of three types of universal assembly vectors.

B. Details of adhesive end switching process of universal cloning vectors through Metclo, using vector VL as an example. Functional type IIS restriction sites are boxed in solid line, blocked restriction sites boxed in dashed line, and methylated bases in bold.

C. Symbolic system for representation of the adhesive end switching process. u and v represents the universal adhesive ends CTCC and CGAG respectively. x and y represents the 4 bp sequence inside the universal adhesive ends defined by the sequence. Assembly using different vector types results in switching of different adhesive ends.

D. Adhesive end switching process as applied to multiple fragments assembly.

FIG. 5. Scarless DNA assembly using universal cloning vectors

A. The DNA fragment to be assembled carries no internal BsaI restriction sites. The aim of the assembly process is to generate a single assembled fragment that carries adhesive ends x and y defined by the ends of the fragment.

B. The sequence is first broken up digitally into 9 different fragments (fragments A-I) with 4 bp overlaps (a-h).

C. Adaptor sequences were then added to the fragments digitally, resulting in DNA fragments that could be trimmed by BsaI to generate fragments flanked by the universal adhesive ends u and v.

D. Synthesized double stranded DNA fragments were first cloned into specific universal assembly vectors to switch the adhesive ends depending on the position of the fragment in the next stage assembly.

E. Cloned fragments were assembled 3 per group into specific universal assembly vectors, with the vector type depending on the position of the assembled fragment in the next stage assembly.

F Final stage of assembly generates the fully assembled fragment with sequence-specified adhesive ends x and y.

FIG. 6. Advantage of Metclo based scarless universal assembly compared to Moclo based universal assembly

For multistage assembly using universal assembly vectors, the Moclo based system uses two different restriction enzymes (BsaI and BpiI) for consecutive stages, which requires different universal adaptors for a given block (CTCC for BsaI and AGAA for BpiI). In order to clone the fragments from different stages into universal assembly vectors, the 5′ end of the first block and the 3′ end of the last block in each subassembly needs to switch between different adaptor sequences depending on the enzyme used for the assembly. The illustration shows the change of the 5′ adaptor for the first block (block A) during 3 consecutive stages of assembly reaction, with BsaI sites boxed in solid line, and BpiI sites boxed in dashed lines. A very long adaptor needs to be added to the 5′ end of block A to cope with the switch of adaptors during the different stages of assembly. The adaptor is “chewed back” 4 bp after each stage of assembly, so the length of the adaptor sequence depends on the number of the stages. The design based on Metclo is much simpler, because it uses a single enzyme BsaI for different stages of assembly. Here nonmethylatable BsaI sites are boxed in solid line, and BsaI sites blocked by methylation boxed in dashed line. The universal adaptor sequence CTCC at the 5′ end of the first block (block A) can be used for different stages of assembly. There is no need to switch adaptor sequence for the outer blocks. As a result the adaptor design for the initial sequence is much simpler.

FIG. 7. DNA assembly using Metclo. The figure illustrates the assembly of four DNA fragments (Fragments A, B, C and D) into a single DNA fragment (Fragment ABCD) through two cycles of Metclo using a single type IIS restriction enzyme. Stage 1 and Stage 2 are effectively the same process with progressively larger inserts. In Stage 1, the insert donor plasmids contain insert fragments (Fragments A, B, C or D) flanked by methylatable type IIS restriction sites that generate compatible adhesive ends. Preparation of insert plasmids in normal E. coli strains, which lack the appropriate site-specific methylase, results in insert fragments releasable by the type IIS restriction enzyme. The assembly vector contains the negative selection marker LacZα flanked by a purposely designed head-to-head configuration of type IIS restriction sites. The inside pair of the restriction sites are designed to be nonmethylatable, but the outside pair are methylatable by a site-specific methylase because the restriction enzyme recognition sequence overlaps with the methylase recognition sequence. Preparation of the assembly vector in E. coli strains expressing the corresponding methylase leads to selective methylation of the outside pair of methylatable restriction sites, and digestion with the type IIS restriction enzyme generates a vector backbone that retains the outside pair of methylated type IIS restriction sites. Ligation of the insert fragments with the vector backbone generates correctly assembled plasmid refractory to restriction by the type IIS restriction enzyme. All of the other unwanted fragments including the vector backbone of the insert plasmid and the LacZα fragment from the assembly vector contain active type IIS restriction sites, which are sensitive to restriction by the enzyme. This scheme enables a one-pot assembly reaction that combines the restriction and ligation step because the reaction favors generation of the correctly assembled plasmid. Transformation of the assembly reaction into a normal E. coli strain, which lacks the appropriate site-specific methylase, effectively results in demethylation of the methylated type IIS restriction sites flanking the assembled fragment in the assembled plasmid. In Stage 2, the assembled inserts (Fragments AB and CD) can then be used for a subsequent round of Metclo assembly into a larger fragment (Fragment ABCD).

FIG. 8. Level jumping: scheme for modification of DNA using components from different levels of assembly. The diagram shows the scheme for assembly of two DNA fragments (Fragments A and B) by the standard Metclo scheme and subsequent modification of the assembled fragment (Fragment AB) using a third fragment (Fragment C). The initial fragments used (Fragments A, B and C) are of the same level in the assembly hierarchy, in the sense that they carry the same antibiotic selection marker. The modification step modifies an existing fragment (Fragment AB) using a fragment (Fragment C) from a different level of assembly, hence “level jumping”. The modified fragment (Fragment ABC) carries the same adhesive ends as the starting fragment (Fragment AB), and an antibiotic selection marker (M3) different from both level 1 and level 2 blocks (M1 and M2). Therefore, the new fragment ABC can be used for the next level of DNA assembly using selection marker M1 in place of fragment AB. The maintenance of the adhesive ends during the modification step is achieved through the use of a special arrangement of head-to-head type IIS restriction sites (to the right of the LacZα fragment during the modification step), in which the inside nonmethylatable type IIS restriction site generates an adhesive end that is compatible with the modification fragment to be inserted (Fragment C), and the outside methylatable type IIS restriction site generates an adhesive end identical to the end of the DNA fragment to be modified (the right-hand end of Fragment AB in the diagram). The modification step is not possible with the standard Moclo approach using two type IIS restriction enzymes, because DNA fragments from consecutive levels of assembly would then need to be released by two different restriction enzymes, each of which needs to be different from the enzyme used for next level of DNA assembly after the modification step. The level-jumping modification scheme is useful for insertion of functional genetic elements such as transcription units into an existing assembly pipeline without changing the higher level assembly design, to maximize the reusability of existing sequence-verified DNA parts.

FIG. 9. Level insertion: scheme for assembly of DNA through an intermediate assembly stage. The diagram shows the scheme for assembly of four DNA fragments (Fragments A, B, C and D) into a single fragment (Fragment ABCD) through an intermediate stage of DNA assembly (Level insertion). In contrast to the standard Metclo assembly scheme (FIG. 3) that assembles the four fragment in a single reaction, this scheme involves an intermediate assembly stage by sub-assembly of fragments B and C into an intermediate assembly vector that carries a selection marker (M3) different from the one for level 1 DNA inserts (M1 carried by fragments A, B, C and D) and for level2 assembled DNA fragment (M2 carried by fragments ABCD). The level-insertion sub-assembly scheme offers more choices in the routes of DNA assembly, and increases the reusablity of intermediate assembled DNA fragments.

FIG. 10. Linear addition: scheme for assembly vector construction using BsaI-based Metclo. The diagram shows the scheme for linear addition of DNA parts for construction of assembly vectors carrying existing functional genetic elements (Fragments A and D). Starting with an assembly vector that carries a nonselectable stuffer DNA fragment flanked by nonmethylatable type IIS restriction sites, two DNA fragments (Fragments A and D) are first assembled with a LacZα selection marker into this vector. The LacZα selection marker is flanked by a specially designed head-to-head arrangement of type IIS restriction sites, such that the inside sites are methylatable and the outside sites are not. Preparation of the LacZα insert plasmid in an E. coli strain expressing the corresponding methylase results in selective blocking of the inside pairs of methylatable type IIS restriction sites. One-pot assembly of the LacZα insert with other inserts prepared from the normal E. coli strain (Fragments A and D) results in an assembled DNA fragment refractory to restriction by the type IIS restriction enzyme used for DNA assembly. Transformation of the assembled DNA into a normal E. coli strain that lacks the corresponding methylase activity results in demethylation of the methylatable type IIS restriction sites flanking the LacZα fragment. As a result, the assembled DNA can then be used as an assembly vector for addition of new DNA fragments (Fragments B and C) in place of the LacZα selection marker in the next round of DNA assembly. The net outcome is linear addition of DNA fragments into the assembly vector (Fragments A and D, then fragments B and C). The scheme is useful for construction of final stage assembly vectors that carry common functional genetic elements, such as mammalian selection markers.

EXAMPLE 1—METHYLATION ASSISTED MODULAR ASSEMBLY USING A SINGLE RESTRICTION ENZYME (METCLO)
Introduction
Overview of Metclo

Here we developed an improved type IIS restriction enzyme-based hierarchical assembly method named Metclo, which reduces the number of restriction enzymes used to one. We achieved this by adding a pair of specific type IIS restriction sites for the same enzyme used for assembly into the assembly vector, which are selectively blocked by DNA methylation. Transformation of assembled DNA into normal strains that lack the specific methylase unblocks the flanking restriction sites, resulting in plasmids carrying the assembled fragment which can be released using the same type IIS restriction enzyme and then used for a subsequent round of DNA assembly. We tested a number of methylases for their abilities to block overlapping methylation/restriction sites for three commonly used commercially available type IIS enzymes BsaI, BpiI and LguI, and identified suitable methylases for each of them, namely M.Osp807II (for BsaI with overlapping sequence GACNNGGTCTCN{circumflex over ( )}NNNN), M2.NmeMC58II (for BpiI with overlapping sequence GAAGACGC{circumflex over ( )}NNNN), and M.XmnI (for LguI with overlapping sequence GAAGCTCTTCN{circumflex over ( )}NNN, methylated base or opposite base is underlined, additional bases required to specify methylase activity in bold, restriction enzyme recognition sequence in italic; all three modifications are m6A N6-methyladenine). We developed E. coli strains carrying chromosomal copy of expression cassettes for constitutive expression of these methylases, and showed that DNA propagated in these strains is refractory to digestion by the restriction enzyme when the restriction site overlaps with the corresponding methylase recognition sequence. We then built proof-of-principle Metclo systems for each pair of methylase/restriction enzyme for assembly of four fragments into a single fragment, which could be released by the same restriction enzyme. Finally, we demonstrated the utility of the M.Osp807II/BsaI-based Metclo system for hierarchical assembly a 218 kb DNA from 28 ×7.7 kb DNA in 2 stages using BsaI.

Materials and Methods
Plasmid Construction

Plasmids were constructed by standard restriction enzyme- and ligation-based cloning techniques. DNA fragments for cloning were generated by PCR or by gene synthesis (IDT or Invitrogen). For proof of principle modular assembly of ˜1 kb DNA from 4 fragments, the modular assembly acceptor vector backbone is based on ampicillin-resistant Moclo vector pICH47732 (available from Addgene, and described in A modular cloning system for standardized assembly of multigene constructs. Weber E, Engler C, Gruetzner R, Werner S, Marillonnet S. PLOS One. 2011 Feb. 18; 6(2):e16765. doi: 10.1371/journal.pone.0016765) and the donor plasmid backbone based on a kanamycin-resistant vector built by replacing the ampicillin-resistant gene in pICH47732 with a kanamycin-resistant gene. For hierarchical assembly of ˜200 kb DNA from 28 fragments, the modular assembly acceptor vectors are based on gentamicin or kanamycin-resistant low copy BAC vector backbone with F replication origin, and the donor plasmid backbone is based on kanamycin-resistant low copy vector backbones with p15A or F replication origin. Plasmids for methylase overexpression were built based on a kanamycin-resistant low copy BAC vector backbone with F replication origin. The methylase genes were under the control of J23100 synthetic promoter, and flanked by a zeocin selection cassette as well as homologous sequences of the arsB locus to facilitate stable integration into the E coli genome by recombineering. Template plasmids for methylase assay are based on amplicillin-resistant pICH47732.

Generation of Methylase Expressing Strains

E. coli strains with stable expression of specific methylases were generated by recombineering. Briefly, DNA fragments containing the methylase gene, zeocin selection marker and flanking arsB homologous sequences were generated by PCR using the corresponding methylase expressing BAC plasmid as template. The fragments were used for lambda red recombineering in DH10B cells followed by zeocin selection to insert the methylase expression cassette into the arsB locus. Clones with correct chromosomal insertions were verified by PCR and sequencing. The methylase expressing strains can be stably maintained without antibiotic selection.

Modular Assembly

For proof of principle modular assembly of ˜1 kb DNA from 4 fragments, assembly reactions were set up using 60 fmol each of donor plasmids prepared from DH5alpha cells, 60 fmol assembly vector prepared from DH10B strains expressing compatible methylase, 1,000U T4 DNA ligase (NEB), and 5U BsaI (NEB) or BpiI (Fermentas) or 2.5U LguI (Fermentas) in 20 μl 1×T4 ligase buffer (NEB). The reaction condition was: 37° C. 15 min, followed by 45 cycles of 37° C. 2 min plus 16° C. 5 min, then 37° C. 20 min, and 80° C. 5 min. Assembled samples were transformed into DH5alpha chemical competent cells, and plated on LB plates with AIX selection (ampicillin 100 μg/ml, IPTG 100 μM, X-Gal 50 μg/ml) at 37° C. overnight. White colonies were expanded and screened by DNA sequencing.

For hierarchical assembly of ˜200 kb DNA from 28 fragments, the assembly was carried out in two stages. At stage 1, the fragments were assembled 7 per group using 15 fmol each of donor plasmids prepared from DH10B cells, 15 fmol assembly vector prepared from DH10B-M.Osp807II cells, 1,000U T4 ligase (NEB), and 5U BsaI (NEB) in 20 μl 1×T4 ligase buffer (NEB). The reaction condition was the same as described above. The assembled samples were then drop-dialyzed against 50 ml dH2O using 0.05 um cellulose filter (Millipore) at room temperature for 1h. 5 μl of dialyzed sample was transformed into 25 μl NEB-10beta eletrocompetent cells by electroporation at 0.9 kV 100Ω 25 μF, using Gene Pulser and 1 mm electroporation cuvettes (Biorad). Following electroporation, cells were recovered by adding 1 ml LB medium and incubated at 37° C. 220 rpm for 1h. Cells were plated on LB plates with GIX selection (gentamicin 2.5 μg/ml, IPTG 100 μM, X-gal 50 μg/ml) at 37° C. overnight. White colonies were expanded and screened by restriction digestion using XhoI.

At stage 2, the ˜200 kb DNA was assembled using protocols similar to stage 1 with the following modifications: 30 fmol each of donor plasmids and 15 fmol assembly vector was used in the assembly reaction; following modular assembly and before the drop dialysis step, 10U BsaI was added to the assembly reaction for incubation at 37° C. for 45 min followed by heat inactivation at 80° C. for 5 min. Transformed cells were plated on LB plates with KIX selection (kanamycin 30 μg/ml, IPTG 100 μM, X-gal 50 μg/ml). White colonies were screened by PCR using primers detecting the junction between the second and the third 54 kb fragments (TACCCACGTGATTCACGCTG and ATCCTACAGACTCGCTGTGG). Selected PCR positive clones were screened by restriction digest using XhoI or BsaI with pulsed-field electrophoresis.

Result

The Metclo system is based on the standard type IIS restriction enzyme-based one-pot DNA assembly system, and added an additional pair of type IIS restriction sites in the assembly vector backbone for release of the assembled DNA for next stage assembly. Unlike the Moclo system which uses restriction sites different from the type IIS enzyme used in the assembly process, Metclo uses the restriction sites for the same enzyme. To prevent the assembled plasmid from being cut by this enzyme in the one-pot assembly reaction, Metclo uses specific methylase to methylate the type IIS restriction sites overlapping with the methylase recognition sequences, in order to block these type IIS restriction sites in the vector backbone. The methylation is lost when the assembled plasmid is transformed into E. coli strains lacking the specific methylase, thus enabling the release of assembled fragment from the assembled plasmid by the same type IIS restriction enzyme used in the assembly reaction. The resulting Metclo system therefore allows hierarchical assembly of DNA fragments using one-pot reaction with a single type IIS restriction enzyme, among various applications.

The key to the Metclo system is the identification of suitable methylase to block type IIS restriction sites that overlap with the methylase recognition sequence in the vector backbone. There are several criteria for selection of suitable methylase. First of all, methylation of the overlapping methylation/restriction site must result in blocking the action of the type IIS restriction enzyme. The methylation process could occur by in vitro methylation of purified vector plasmid, or by propagating vector plasmid in strains that express the methylase in vivo. In vivo methylation is preferred due to the reduced cost and time in the vector preparation process. Secondly, the methylase recognition sequence must not be identical to or entirely enclosed by the type IIS restriction site; additional base pairs must be required to specify the action of the methylase on the overlapping methylation/restriction site. The reason for this requirement is because there are two pairs of type IIS restriction sites for the same enzyme in the assembly vector. One pair within the vector backbone need to be blocked by the methylase, whereas the other pair within the negative selection marker insert must not be blocked. If the methylase recognition sequence is identical to, or enclosed by the type IIS restriction site, all the restriction sites in the assembly vector for this particular type II restriction enzyme will be blocked by methylation, including the pair within the negative selection marker insert; the resulting methylated assembly vector won't be cut by the type IIS enzyme at all, therefore cannot be used for DNA assembly. Thirdly, additional base pairs to specify methylase activity should not overlap with the adhesive end sequence generated by the type IIS restriction enzyme. This will maintain maximum flexibility in design of adaptor sequence for DNA assembly.

We searched for methylases with predicted ability to block restriction sites for type IIS restriction enzymes BsaI, BpiI and LguI. These are common type IIS restriction enzymes used in type IIS-based assembly systems. We used a few extra criteria in addition to the ones listed above to choose candidate methylases for testing. We aimed to generate methylated plasmids by in vivo methylation, so the methylase should ideally be active at the growth temperature of E coli. We also preferred methylases that recognize longer sequences (5-6 bp), because with longer recognition sequences, fewer bases in the E coli host genome and assembly vector backbone would serve as methylation substrate by chance. This potentially increases the efficiency of methylation, and reduces the chance of adverse effect of heterologous DNA methylation on host gene function. In addition, we preferred single subunit methylases from type II restriction/modification systems that lack restriction activity towards unmethylated sequence to avoid potential problem of restriction during the vector construction step. Lastly, to increase the chance of methylation blocking the overlapping methylation/restriction sites, we preferred methylases that modify bases close to the middle of the restriction site.

Based on these criteria, we selected the following methylases for testing: M.Osp807II (GACNNGGTCTC m6A) for BsaI, M.Rsp7740I (TTCGAAGAC m6A) and M2.NmeMC58II (GAAGACGC m6A) for BpiI, and M.SacI (GAGCTCTTC m5C), M.AspJHL3I (GAGCTCTTC m4C) and M.XmnI (GAAGCTCTTC m6A) for LguI (methylated base or complementary base underlined). We screened methylase activity by preparing ColE-based (i.e. ColE-derived) high copy template plasmid carrying overlapping methylation/restriction sites in DH10B cells expressing the corresponding methylase under the J23100 constitutive synthetic promoter from a low copy BAC vector (low copy BAC vector backbone with F replication origin). This identified M.Osp807II (for BsaI), M2.NmeMC58II (for BpiI) and M.XmnI (for LguI) as suitable candidates for further analysis (FIG. 1A). We then integrated the methylase expression cassette with a zeocin selection marker into the arsB locus using lambda-red recombineering to generate stable strains overexpressing the methylase from a chromosomal copy (Microb Cell Fact. 2013 Jun. 24; 12:60. doi: 10.1186/1475-2859-12-60 (PMID 23799955)), and tested the ability of these methylases to block overlapping methylation/restriction sites in vivo. The test vector carries one restriction site that does not overlap with methylation sequence, thus permissible for restriction in both wild-type and methylase-expressing strain, and one site with two head-to-head type IIS restriction sites, both of which overlap with the corresponding methylation sequence and would, therefore, be blocked in the strain expressing the methylase. The head-to-head arrangement allows for assessment of nickase activity as well as double strand restriction activity in the same assay (FIG. 1B). Following 1h digestion of 60 fmol test plasmid (pMOP_BsaINC, pMOP_BpiINC and pMOP_LguINC) in 10 μl reaction using 5U (BsaI, BpiI) or 2.5U (LguI) enzyme at 37° C., DNA were analyzed by agarose gel electrophoresis with EtBr staining. The result shows that under test condition, the three methylases block restriction of the overlapping sites, as demonstrated by the lack of generation of ˜600 bp fragment (FIG. 1C).

We then developed proof-of-principle Metclo DNA assembly systems using these methylases for one-pot DNA assembly (FIG. 2 shows BsaI-MOsp807II as an example). In these systems, the donor DNA inserts to be assembled are flanked by overlapping methylation/restriction site. Because the donor plasmids containing the DNA inserts to be assembled are prepared in a normal E coli strain that lacks the corresponding methylase, the restriction site is not blocked and the donor insert could be released by the enzyme in the assembly reaction. The assembly vector carries a different selection marker from the donor plasmid, and a LacZ selection marker flanked by head-to-head typeIIS restriction sites. For each head-to-head site, the two type IIS restriction sites generate the same adhesive end. The outside site close to the vector backbone overlaps with the methylation sequence, and the inside site close to the LacZ insert does not. The destination vectors are prepared from an E coli strain that expresses the corresponding methylase. As a result, only the inside typeIIS sites are functional in the assembly reaction. During the reaction, the LacZ insert is released from the vector backbone due to action of enzyme on the inside typeIIS site; ligation of the donor insert with the destination vector results in typeIIS restriction sites within from the destination vector backbone, which is blocked by methylation; religation of the freed LacZ insert with the destination vector backbone results in regeneration of the head-to-head typeIIS site, which is sensitive to restriction due to the inside site not blocked. As a result, the reaction favors ligation of donor fragments to destination vector backbone. The moclo reaction is then transformed into normal E coli strain that lacks the specific methylase activity for screening for LacZ expression using LB plates containing IPTG and X-Gal. The white colonies that lack LacZ activity should contain successfully assembled plasmids. The assembled DNA fragment have flanking type IIS restriction sites that overlap with methylase recognition sequences, which are identical to the donor vector. As a result, this allows a further round of modular assembly at the next level using the same restriction enzyme and so on for multiple rounds as required.

We tested the systems for the three enzymes for assembly from four fragments (Table 1). The donor vectors are kanamycin-resistant, and destination vectors ampicillin-resistant. Destination vectors were prepared from corresponding methylase-expressing strains. Following selection, 8 white colonies were picked for miniprep and analyzed by Sanger sequencing. Sequencing result shows that 100% (8/8) of clones are correct for each of the three Metclo systems (Table 1). This confirms the utility of Metclo for modular assembly using a single restriction enzyme such as BsaI, BpiI or LguI.

To further explore the utility of Metclo for hierarchical assembly of large DNA constructs, we assembled a 218 kb DNA fragment from 28×7.7 kb fragments using BsaI restriction enzyme in two stages. In stage one, 28 DNA fragments of 7.7 kb which were free of BsaI sites were assembled 7 per group into 4 pieces of 54 kb fragments using BsaI-based Metclo system. In stage two, the 4 pieces of 54 kb fragments were assembled into a single 218 kb fragment. The final 218 kb fragment is composed of non-repetitive sequence with ˜48% GC content. Restriction digest shows a ˜50% success rate for stage 1 assembly (2/4 to 3/4 clones correct), and ˜20% for stage 2 assembly (14 out of 27 PCR verified, of which 3 out of 8 verified by restriction digest) (Table 2). This confirms that the Metclo system can be used for hierarchical assembly of large DNA construct using a single type IIS restriction enzyme.

Discussion

Here we present Metclo, a modified type IIS restriction enzyme based DNA assembly system for one-pot assembly of DNA using common type IIS restriction enzymes such as BsaI, BpiI or LguI, that generates assembled DNA releasable by the same restriction enzyme. This enables hierarchical assembly of large DNA constructs using a single type IIS restriction enzyme. The system uses methylase-overexpressing strains during the vector preparation step for in vivo methylation of overlapping methylation/restriction sites to block selective restriction sites in the vector. The assembly process is the same as the type IIS DNA assembly systems through the use of one-pot reaction, thus preserves the simplicity of the existing systems. The part preparation and transformation steps utilize normal strains similar to the standard Moclo system, allowing the use of routine cloning strains for DNA assembly, similar to the current type IIS assembly systems.

The Metclo system has a number of advantages over the existing type IIS restriction enzyme-based hierarchical DNA assembly systems. Firstly, the Metclo approach reduces the burden to remove internal restriction sites during component part construction. Compared with the standard Moclo system that uses two or more enzymes, the BsaI- and BpiI-based single enzyme Metclo system uses a 6 bp cutter that reduces the frequency of forbidden restriction sites from 1 per ˜1 kb to 1 per ˜2 kb for random DNA with 50% GC content, and the LguI-based Metclo system uses a 7 bp cutter that reduces the frequency further to 1 per ˜8 kb.

Metclo improves the flexibility of the hierarchical type IIS assembly system. Because assembled DNA fragments from different stages of assembly can be released by the same restriction enzyme, with appropriate design of adaptors and choice of antibiotic resistant vector, the system potentially allows design of several assembly schemes not achievable using the existing system. These include level jumping—the assembly of DNA using parts from different stages of assembly—and level insertion—the assembly of DNA fragments through an intermediate assembly stage. These schemes are useful for modification of large DNA constructs using existing parts, and for subassembly of complex parts such as multi-part open reading frames for added reusability.

Additional schemes could also be devised for linear addition of DNA parts during the assembly process. For example, DNA parts containing selection markers flanked by internal methylation-blocked type IIS restriction sites could be used during DNA assembly. The assembled plasmid could then be used as an assembly vector for insertion of additional DNA parts in place of the selection marker in the next stage assembly. This is particularly useful for construction of common assembly vectors containing functional modules.

Metclo also increases the exchangeability of parts from different assembly standards. Independent development of part library and assembly standards lead to assembly systems that use different type IIS restriction enzymes. For example, for plant biology, Moclo uses BsaI/BpiI (and Esp3I), Goldenbraid uses BsaI and BsmBI (and BtgZI); for E. coli, Ecoflex uses BsaI/BsmBI, CIDAR uses BsaI/BpiI. This place constraints on exchangeability of parts among different collections. The Metclo system that uses a single type IIS restriction enzyme is compatible with any system that uses that restriction enzyme. In particular, the BsaI-based system is compatible with all the existing systems that use BsaI. Given that BsaI has been used in most of the type IIS restriction enzyme-based hierarchical modular assembly systems to date (ACS Synth Biol. 2016 Oct. 21; 5(10):1059-1069 (PMID 27096716); ACS Synth Biol. 2016 Jan. 15; 5(1):99-103. doi: 10.1021/acssynbio.5b00124 (PMID 26479688; ACS Synth Biol. 2015 Sep. 18; 4(9):975-86. doi: 10.1021/sb500366v (PMID 25871405); and PLOS One. 2011 Feb. 18; 6(2):e16765. doi: 10.1371/journal.pone.0016765 (PMID 21364738), the BsaI-based Metclo system has potential to be used as a standard for modular assembly that is compatible with most of the existing part libraries.

There are DNA assembly systems that use DNA methylation to block internal type IIS restriction sites during the assembly process. For example, the TNT assembly system (Sci Rep. 2016 Jan. 13; 6:19278. doi: 10.1038/srep19278 (PMID 26758940)) uses M.TaqI to methylate overlapping Earl recognition site, which results in an assembly system using 7 bp cutter LguI (GCTCTTCN{circumflex over ( )}NNN) and 6 bp cutter Earl (CTCTTCN{circumflex over ( )}NNN) both giving 3 bp sticky ends, thus requiring removal of a single 6 bp sequence from DNA parts (CTCTTC). However, because the M.TaqI recognition sequence (TCGA) that overlaps with the Earl restriction site intrudes the adhesive end sequence (CTCTTCG{circumflex over ( )}ANN), the system places limitations on the design of adaptors, thus could only assembly fragments three at a time, and could not be expanded to an LguI-only assembly system. As another example, Greengate used methylated linker DNA as parts during the DNA assembly process to introduce new BsaI sites for subsequent rounds of assembly. The resulting system however could only be used for linear addition of DNA parts, thus limiting the use of the system to construction of DNA constructs with a small number of transcription units. Compared to these systems, the Metclo system, with the use of in vivo methylation of overlapping methylation/restriction site outside the adaptor sequence, preserves the advantage of the hierarchical multiplicative assembly scheme of the Moclo system, and maintains the freedom of choice of adaptor sequences for DNA assembly. In practice, similar result could be achieved by adding methylated linker DNA as the first and last parts during the assembly process. However, this would increase the number of parts in the assembly by two, which reduces success rate of assembly compared to the in vivo vector methylation approach in the Metclo system.

In summary, the Metclo system, through the use of in vivo methylation to block specific type IIS restriction sites within the assembly vector, enables hierarchical assembly of large DNA fragments using a single type IIS restriction enzyme. The system simplifies the existing type IIS restriction enzyme-based assembly method and increases the flexibility of design of assembly schemes. The BsaI-based Metclo system in particular is compatible with all the existing part libraries that uses BsaI, and could be used as a standard type IIS-based assembly system.

TABLE 1

Proof of principle assembly of DNA fragment using BsaI, BpiI or Lgui-based Metclo systems

Table 1. Proof of principle assembly of DNA fragment using BsaI, BpiI or Lgui-based Metclo systems

Plasmid
Vector
Inserts
Methylase
Enzmye
Size
Success rate

pMXP_test
pMXK2_A4E
pMXP_FragA pMXP_FragB pMXP_FragC pMXP_FragD
M.Osp807II
BsaI
908 bp
8/8

pMYP_test
pMYK2_A5E
pMYP_FragA pMYP_FragB pMYP_FragC pMYP_FragD
M2.NmeMC58II
BpiI
1152 bp
8/8

pMZP_test
pMZK2_P6T
pMZP_FragA pMZP_FragB pMZP_FragC pMZP_FragD
M.Xmnl
LguI
1149 bp
8/8

TABLE 2

Hierarchical assembly of a 218 kb DNA fragment using BsaI-based Metclo system

Success

Plasmid
Vector
Inserts
Size
rate

pMXBG_3A1
pMXBG_A7I
pMOLK_2A1 pMOLK_2B1 pMOLK_2C1 pMOLK_2D1 pMOLK_2E1
54 kb
3/4

pMOLK_2F1 pMOLK_2G1

pMXBG_3I1
pMXBG_I7G
pMOLK_2I1 pMOLK_2A2 pMOLK_2B2 pMOLK_2C2 pMOLK_2D2
55 kb
2/4

pMOBK_2E2 pMOLK_2F2

pMXBG_3G1
pMXBG_G7F
pMOLK_2G2 pMOLK_2I2 pMOLK_2A3 pMOLK_2B3 pMOLK_2C3
54 kb
2/4

pMOBK_2D3 pMOLK_2E3

pMXBG_3F1
pMXBG_F7E
pMOLK_2F3 pMOLK_2G3 pMOLK_2I3 pMOLK_2A4 pMOLK_2B4
54 kb
2/4

pMOLK_2C4 pMOLK_2D4

pMXBK_4A1
pMXBK_A7E
pMXBG_3A1 pMXBG_3I1 pMXBG_3G1 pMXBG_3F1
218 kb
3/8*

*14/27 PCR positive, of which 3/8 verified by restriction digest

Example of an Insertional Composite Element

In another scenario, longer DNA sequences could be included between the methylation-protectable restriction site and the opposing non-protectable restriction site in the assembly vector, such that following DNA assembly, these sequences will be added into the 5′ or 3′ end of the assembled DNA plasmid. This design is convenient for adding common elements to the assembled plasmid by using specialized vectors.

An example is shown in FIG. 3 for assembly of a transcription unit for expression of a protein with three different domains, starting from insert plasmids containing the three individual domains D1, D2 and D3 using BsaI-based Metclo. Figure A shows the Metclo assembly using a special Metclo vector containing a promoter between the methylation-protectable restriction site and the non-protectable restriction site at 5′ of the LacZ (discard sequence), and a polyA element at 3′ (“pre-embedded vector”. Metclo using this special pre-embedded vector allows the assembly of the three domains into a transcription unit in a one-pot reaction from three insert plasmids, and the assembled transcription unit consisting a promoter, a coding region (D1 D2 and D3), and a polyA signal could be released from the assembled plasmid for next round of Metclo assembly. This is in contrast with the standard Metclo using “maintained composite element”, whereas the transcription unit has to be assembled from 5 different insert plasmids (Figure B). In the figures XXXX and YYYY represents the adaptor sequence at 5′ and 3′end of the assembled DNA fragment (transcription unit) that could be released for next round of DNA assembly. For DNA assembly using “pre-embedded vectors”, these two adaptor sequences can be the same as internal adaptor sequences because they are not involved in the one pot three fragment assembly. For DNA assembly using standard Metclo vector though, XXXX and YYYY adaptor sequences must be different from the internal adaptor sequences (AATG, ACTA, ATCT and GCTT). In addition, the method using pre-embedded vectors (Figure A) has advantage that less number of DNA parts need to be assembled in a single reaction, which increases the rate of success. The disadvantage is that the specialized vector is more difficult to prepare.

EXAMPLE 2—SCARLESS DNA ASSEMBLY (SCARLESS METCLO)

A unique application of Metclo is a system for scarless hierarchical assembly of large DNA construct using a limited set of universal cloning vectors. Type IIS based DNA assembly using universal vectors relies on a specific arrangement of head-to-head type IIS restriction sites in the assembly vector that changes the adhesive end of the cloned fragment. Scarless assembly is achieved by using these universal vectors to generate assembled DNA fragments with adhesive ends (scars) defined by the DNA fragment for subsequent round of assembly, which buried the assembly scar into the assembled sequence. The use of the Metclo system allows maintenance of the same universal adhesive ends for the outer fragments during different stages of hierarchical assembly. A combination of these three features results in a universal scarless assembly system.

Here we describe the principles of scarless Metclo assembly system using the BsaI restriction enzyme. The system relies on a set of three types of universal cloning vectors that change the adhesive ends of the cloned fragments (FIG. 4A). The three vector types are named VL, VM and VR. The vectors are designed to clone DNA fragments with adhesive end CTCC at 5′ end and CGAG at 3′ end could be cloned into the above vectors. The cloned fragment, once released by BsaI from the vector, will have different adhesive ends depending on the vector used. Vector VL is designed to keep the 5′ adhesive end, and change the 3′ adhesive end to the four bases inside the 3′ CGAG sequence; Vector VR to keep the 3′ adhesive end, and change the 5′ adhesive end to the four bases inside the 5′ CTCC sequence; Vector VM to change both ends to the four bases inside. In this way a set of universal vectors could be used to generate DNA fragments with adhesive ends defined by the insert sequence.

The detail of adhesive end switching process is described in FIG. 4B using vector VL as an example. When the vector is prepared in methylase-expressing competent cells, methylation of adenine (bold) due to overlapping methylase recognition sequence results in blocking of the outside BsaI sites (boxed in dotted line), where the inside BsaI sites (boxed in solid line) are available to remove the LacZ insert. Note that the left arm of vector VL is similar to standard Metclo, that both the outside and inside BsaI sites give rise to the same adhesive end CTCC. The right arm of VL however is different that the adhesive end CGAG generated by the inside BsaI site overlaps with the outside BsaI site. The donor DNA carries an insert that could be released by flanking BsaI sites with compatible universal adhesive ends CTCC and CGAG. The double strand insert carries 4 bp sequence XXXX on the 5′ end and YYYY on the 3′ end inside the universal adhesive end. Following Metclo assembly of the donor DNA into vector VL, the resulting plasmid carries the corresponding insert that could be released by the flanking BsaI sites (boxed in solid line) in the vector. The released fragment would carry a 5′ adhesive end CTCC identical to the one used for cloning, but the 3′ adhesive end would be YYYY as defined by the insert sequence, which may be different from the universal 3′ adhesive end CGAG. In this way, vector VL switches the right hand adhesive end of the cloned fragment from universal adhesive end CGAG to the sequence-defined adhesive end YYYY.

The conversion process using the three types of universal cloning vectors could be described using a simple symbolic representation system (FIG. 41C). Here we use letter u to represent the 5′ universal adhesive end CTCC, and letter v to represent the 3′ universal adhesive end CGAG. x and y represents the 4 bp sequence at the 5′ and 3′end of the insert fragment inside the universal adhesive end. Insert (u,v) represents a plasmid carrying insert fragments flanked by BsaI sites that generate adhesive ends u and v. Assembly reaction with different vectors results in different adhesive ends being converted.

The same set of universal vectors could be used to assemble multiple fragments with compatible adhesive ends, as long as the 5′ end of the first fragment and the 3′ end of the last fragment have universal adhesive ends u and v (FIG. 4D). The assembled fragment carries different adhesive ends depending on the type of assembly vector used. Assembly of fragments into vector VL keeps the 5′ universal adhesive end, and converts the 3′ end into the internal 4 bp. Assembly into vector VM converts both ends, and assembly into vector VR converts the 5′ end and keeps the 3′ universal adhesive end. Note the fragment assembled into vector VL resembles fragment A that it carries only 5′ universal adhesive end u; the fragment assembled in vector VM resembles fragment B; and the fragment assembled in vector VR resembles fragment C. Therefore the process is self-similar, such that the assembled fragments could be used for next round DNA assembly using the same principle depending on the position of the fragment in the assembly order. This enables hierarchical scarless assembly of large DNA constructs using universal cloning vectors.

The whole process of hierarchical scarless assembly of DNA is described in FIG. 2, using DNA assembly from 9 fragments in 2 stages as an example. Starting with a DNA sequence free of internal BsaI sites, with the 4 bp at 5′ end named x, and 3′end named y, the aim is to assemble the DNA from 9 fragments into a single fragment that once released from the assembled plasmid carries adhesive ends x and y (FIG. 5A). The DNA sequence is first broken up into 9 fragments named A-I with 4 bp overlapping sequences, with the overlapping sequences named a-h (FIG. 5B). The positions of 4 bp overlaps could be chosen freely, as long as they are different to universal adhesive ends u and v, and to each other within each subassembly reaction. For the current set up, the following pairs of 4 bp overlaps should be different within each pair: (a,b), (d,e), (g,h) and (c,f). Appropriate adaptor sequences were added to the ends of sequences of fragments A-I, and double DNA synthesized by gene synthesis or PCR, so that following restriction digest with BsaI, the double stranded DNA could be trimmed into fragments A-I carrying universal adhesive ends u and v (FIG. 5C). These double stranded synthetic DNA fragments could then be cloned into appropriate types of universal cloning vectors to change the adhesive end of the fragment depending on the position of the fragment in the next stage assembly (FIG. 5D). Fragments A, D and G, which will locate at the 5′ end position in the next round of assembly, are cloned into vector type VL; B, E, H in the middle position in the next round are cloned into vector type VM; C, F, I at 3′ end position into vector type VR. Cloned fragments could be sequenced at this stage to check for errors in gene synthesis or PCR. These fragments could then be used for a new round of assembly, and again the type of assembly vector used depends on the position of the assembled fragment in the next round DNA assembly (FIG. 5E). Fragments A, B, C were assembled into vector VL, because the assembled fragment ABC will be used as the 5′ fragment in the next round assembly; fragments D, E, F into vector VM, and fragments G, H, I into vector VR. The final step is similar so that the three assembled fragments from step 2 is further assembled into vector VM, which generates the fully assembled fragment that carries adhesive ends x and y defined by the ends of the initial sequence (FIG. 5F).

A set of at least 6 universal cloning vectors representing 3 types (VL, VM, VR) and 2 antibiotic selection markers are required for hierarchical assembly. Ideally 12 vectors representing 2 different copy numbers are optimal in order to cope with different sizes of DNA fragments at different stages of assembly. Similar system could be developed for LguI based Metclo, but not BpiI, because the scarless system requires universal adaptor u and v to be different. For BsaI and SapI, the bases between the enzyme recognition sequence and adhesive end generated by the enzyme is flexible, therefore it is possible to design different adaptors u and v. For BpiI however, these bases are fixed because the two bases are both required to specify the methylase recognition sequence in BpiI based Metclo. In addition, the methylated base of BpiI based Metclo is located on the top strand of the restriction site. Head-to-head arrangement of BpiI sites for adhesive end switching inevitably results in the methylated base being carried by the LacZ selection marker, not the assembly vector backbone following BpiI digestion, which makes one pot Golden Gate assembly impossible.

The Relationships Between Scarless Metclo and Metclo

Metclo was invented to solve the problem of using a single restriction enzyme for type IIS restriction enzyme-based hierarchical assembly of large DNA construct. Scarless Metclo is a further development of Metclo that utilizes key components of the Metclo system (methylase-expressing strains, overlapping methylation/restriction site) to achieve scarless hierarchical assembly of large DNA constructs using a set of universal assembly vectors.

Scarless Metclo is based on a combination of several different concepts of type IIS-based assembly system:

- 1. A special design of head-to-head type IIS restriction site for adhesive end switching, which has been used in the Moclo system for “level-1” universal cloning vector design. The Moclo “level-1” vector design uses a BpiI-BsaI head-to-head restriction site, which is different from the BsaI-only head-to-head design in the Metclo, but the concept of adhesive end switching is based on the Moclo “level-1” vector design.
- 2. The removal of the assembly scar by designing the scar sequence around the assembled DNA sequence. This hasn't been demonstrated in type IIS-based hierarchical assembly before.
- 3. The use of Metclo system to maintain the same adhesive end sequences for the outer blocks of DNA fragments for each stage of assembly.

The use of Metclo system is key to achieve multi-stage hierarchical assembly using the same universal adhesive end sequence. The first two concepts alone are sufficient for scarless single stage assembly of DNA using a universal assembly vector. The single stage scarless assembly system however could not be adapted easily for multi-stage hierarchical assembly using the traditional Moclo system, because the universal adaptor sequence of the adhesive end switching vector design for different enzymes are different (BsaI would be CTCN and NGAG, and BpiI ACNN and NNGT). This means that in order to achieve hierarchical scarless assembly using the two enzyme-based Moclo, very long repetitive adaptor sequences need to be added to the ends of the outer blocks depending on the number of stages of assembly, which will be “chewed back” following each stage of assembly (FIG. 6). Because Metclo system uses a single enzyme for different stages of assembly, the same universal adaptor sequence could be used for the outer blocks during different stages of assembly, which greatly simplifies the design of the assembly process.

EXAMPLE 3—IDENTIFIED METHYLASE AND TYPE IIS RESTRICTION ENZYME COMBINATIONS

Table 3 contains a list of methylase/restriction enzyme combinations that may result in blocking of overlapping methylation/restriction site for all known type IIS restriction enzymes (78 enzymes representing 19 different specificity).

The list was generated using a custom Perl script based on a list of known type IIS restriction enzymes and a list of known type II DNA methylases, both from Rebase (PMID 25378308. (Nucleic Acids Res. 2015 43: D298-9).

The listed methylases are limited to methylases from type II restriction/modification systems with recognition sequences of at least 4 bp and for which the position of the methylated base is known. Methylases from the type IIG restriction/modification system that contain both methylase and restriction enzyme activity in the same protein are excluded. In addition to the restriction enzyme recognition sequence, further bases must be required to specify the methylase se activity towards the overlapping methylation/restriction site. Any methylase whose recognition sequence is identical to or enclosed by the restriction site are removed. In addition, the methylation sequence must not intrude into the adhesive end sequence generated by the type IIS restriction enzyme.

The DNA sequences are listed using the IUPAC Ambiguity Codes. M represents A or C; R represents A or G; W represents A or T; S represents C or G; Y represents C or T; K represents G or T; V represents A or C or G; H represents A or C or T; D represents A or G or T; B represents C or G or T; N represents A or T or C or G.

‘Restriction enzyme’ lists the name of type IIS restriction enzyme. Restriction enzymes that recognize the same sequence are grouped together.

‘Restriction site’ lists the recognition sequence of the restriction enzyme.

‘Methylase’ listed the name of the methylase that could block the overlapping methylation/restriction site. Methylases that recognize the same sequence and generate the same pattern of methylation are grouped together.

‘Methylation sequence’ lists the recognition sequence of the methylase.

‘Position of methylated base’ listed the position of the methylated base or opposite base in the methylase recognition sequence.

‘Type of methylation’ lists the types of methylation for the methylated bases in the order of the positions of methylated bases listed in the previous column. m6A represents N6-methyladenine; m5C represents C5-methylcytosine; m4C represents N4-methylcytosine; ‘Unknown’ represents unknown type of methylation.

‘Overlapping methylation/restriction site’ lists the sequence of the overlapping methylation/restriction site that may result in blocking of the action of listed restriction enzyme through methylation by the listed methylase. For some pairs of restriction enzyme/methylase combination, there are several different overlapping methylation/restriction sites to choose from. The bases bracketed in ‘[ ]’ represent the adhesive ends generated by the restriction enzyme.

TABLE 3

Posi-

tion

Re-

of

stric-

Methyla-
methyl-
Type of

Restriction
tion

tion
ated
methyl-
Overlapping methylation/

enzyme
site
Methylase
sequence
base
ation
restriction site

Sth132I
CCCG
M.AciI
CCGC
1, 3
m5C
CCCGCNNN[NNNN]

Sth132I
CCCG
M.Ama55I, M.CnaPQ2III, M.Rmo1926II,
CGGCCG
1, 6
m5C
CCCGGCCG[NNNN]

M.SenC1765IV, M.SspG1I, M.XorPXII

Sth132I
CCCG
M.ApaI
GGGCCC
3, 4
m5C
GGGCCCGNNNN[NNNN]

Sth132I
CCCG
M.AquI
CYCGRG
1, 6
m5C
CCCGRGNN[NNNN]

Sth132I
CCCG
M.AspDUT2 II, M.BstYI, M.CagIII,
RGATCY
2, 5
m4C
RGATCCCGNNNN[NNNN]

M.Mpa1757V, M.Mru1279V, M.RsaIII,

M.XhoII

Sth132I
CCCG
M.AspWA102I, M.AvaVII, M.BceJII, M.CbeI,
GGCC
2, 3
m4C
GGCCCGNNNN[NNNN]

M.Ckr177II, M.CmaLM2IV, M.CpeAVII,

M.Csp323II, M.PhoI, M.Ssp6714III, M.SuaI

Sth132I
CCCG
M.AvaV, M.SliSI
GATC
1, 4
m4C
GATCCCGNNNN[NNNN],

CCCGATCN[NNNN]

Sth1321
CCCG
M.BamHI
GGATCC
2, 5
m4C
GGATCCCGNNNN[NNNN]

Sth132I
CCCG
M.BanII, M.EcoT38I
GRGCYC
3, 4
m5C
GRGCCCGNNNN[NNNN]

Sth132I
CCCG
M.BanLII, M.CpeAII, M.SinI
GGWCC
2, 4
m5C
GGWCCCGNNNN[NNNN]

Sth132I
CCCG
M.BceSII
GGWCC
1, 5
m5C
GGWCCCCGNNNN[NNNN],

GGWCCCGNNNN[NNNN],

CCCGGWCC[NNNN]

Sth132I
CCCG
M.BepI, M.FpsJV, M.Gel16401III, M.Pdi8503I
CGCG
1, 4
m5C
CCCGCGNN[NNNN]

Sth132I
CCCG
M.Bgl2196III
CGTACG
2, 5
m4C
CCCGTACG[NNNN]

Sth132I
CCCG
M.BhaII, M.BspRI, M.BsuRI, M.CthVI,
GGCC
2, 3
m5C
GGCCCGNNNN[NNNN]

M.DthLII, M.HaeIII, M.MthTI, M.NgoAII,

M.NgoPII, M.Pod15391I

Sth132I
CCCG
M.BsoBI
CYCGRG
1, 6
m4C
CCCGRGNN[NNNN]

Sth132I
CCCG
M.Bsp14III, M.Bsp3003I, M.Bsp3004I,
GATC
1, 4
m5C
GATCCCGNNNN[NNNN],

M.CalB3III, M.CpeAIII, M.Esu35585I,

CCCGATCN[NNNN]

M.FpsJIV, M.Lba2031I, M.Lbo2004I,

M.LlaKR2I, M.Lmo1042I, M.Lmo7956II,

M.Lsp10393II, M.MspI7I, M.Sau3AI, M.Sba3I,

M.Sin395I, M.Spn23FI, M.Sth368I

Sth132I
CCCG
M.BsuFI, M.CpeAIV, M.MspI
CCGG
1, 4
m5C
CCCGGNNN[NNNN]

Sth132I
CCCG
M.Cac8I
GCNNGC
2, 5
m5C
GCCCGCNNN[NNNN]

Sth132I
CCCG
M.Cdi630III
CCSSGG
2, 5
m4C
CCCCGGNNN[NNNN], CCCGGGNN[NNNN],

CCCGSGGN[NNNN]

Sth132I
CCCG
M.Cfr9I, M.SmaI, M.Sma36365II, M.Xca85III,
CCCGGG
2, 5
m4C
CCCGGGNN[NNNN]

M.XcyI, M.XmaI, M.XveII

Sth132I
CCCG
M.CocIII, M.Fsi4947II, M.TdeIII,
GGNCC
1, 5
m4C
GGNCCCCGNNNN[NNNN],

M.Tph12270I

GGNCCCGNNNN[NNNN],

GGCCCGNNNN[NNNN], CCCGGNCC[NNNN]

Sth132I
CCCG
M.CviJI
RGCB
2, 3
m5C
CCCGCYNN[NNNN], VGCCCGNNNN[NNNN]

Sth132I
CCCG
M.Dac11109II, M.Hfo6591I, M.MhuII,
GTAC
1, 4
m4C
GTACCCGNNNN[NNNN],

M.MjaV, M.MlaZII, M.MspLW5I, M.RsaI

CCCGTACN[NNNN]

Sth132I
CCCG
M.Djo10085II, M.Ssp6803II
GGCC
2, 3
Unknown
GGCCCGNNNN[NNNN]

Sth132I
CCCG
M.DsaV, M.Ecl18kI, M.FpsJVI, M.NlaX,
CCNGG
2, 4
m5C
CCCGGNNN[NNNN]

M.SenPI, M.SsoII

Sth132I
CCCG
M.Eco29kI, M.NgoAIII, M.SacII
CCGCGG
2, 5
m5C
CCCGCGGN[NNNN]

Sth132I
CCCG
M.EcoO109I
RGGNCCY
3, 5
m5C
RGGNCCCGNNNN[NNNN]

Sth132I
CCCG
M.EfaRFII, M.Mae7806II
CCGG
2, 3
m4C
CCCGGNNN[NNNN]

Sth132I
CCCG
M.FisAW1I, M.ThaI, M.Tph12270II
CGCG
1, 4
m4C
CCCGCGNN[NNNN]

Sth132I
CCCG
M.FnoB1II, M.Hal24586I, M.Hpy298V,
CCGG
1, 4
m4C
CCCGGNNN[NNNN]

M.Hpy299IX, M.Hpy30V, M.Hpy32XI,

M.Hpy57V, M.Hpy66IX, M.Hpy99VIII,

M.HpyFV, M.HpyUM032IX, M.HpyUM037IX,

M.MjaVI, M.PspJDRI, M.TmeBII

Sth132I
CCCG
M.FpsJI, M.HpaII, M.Nme18II, M.NmeAI
CCGG
2, 3
m5C
CCCGGNNN[NNNN]

Sth1321
CCCG
M.HgiCI
GGYRCC
2, 5
m5C
GGYRCCCGNNNN[NNNN]

Sth132I
CCCG
M.Hpy66I, M.Hpy991
CGWCG
2, 4
m4C
CCCGWCGN[NNNN]

Sth132I
CCCG
M.Hpy99IV, M.HpyFORFX
CCNNGG
1, 6
m4C
CCCGGGNN[NNNN], CCCGNGGN[NNNN]

Sth132I
CCCG
M.Mae7806III, M.PspPI, M.Sau96I
GGNCC
2, 4
m5C
GGNCCCGNNNN[NNNN],

GGCCCGNNNN[NNNN]

Sth132I
CCCG
M.MjaII
GGNCC
1, 5
m5C
GGNCCCCGNNNN[NNNN],

GGNCCCGNNNN[NNNN],

GGCCCGNNNN[NNNN], CCCGGNCC[NNNN]

Sth132I
CCCG
M.MpeII
RGCY
2, 3
m5C
RGCCCGNNNN[NNNN]

Sth132I
CCCG
M.NgoAXV, M.NgoMV
GGNNCC
2, 5
m5C
GGNNCCCGNNNN[NNNN],

GGNCCCGNNNN[NNNN]

Sth132I
CCCG
M.NheI
GCTAGC
1, 6
m4C
GCTAGCCCGNNNN[NNNN]

Sth132I
CCCG
M.NmeDI
VCCGGY
2, 5
m5C
CCCGGYNN[NNNN]

Sth132I
CCCG
M.Pfa23572II
CGATCG
1, 6
m5C
CCCGATCG[NNNN]

Sth132I
CCCG
M.Psp737I
CCCCCD
4
m4C
CCCCCGNNNN[NNNN]

Sth132I
CCCG
M.RpaV
GCCCGCC
4
m4C
GCCCGCCNN[NNNN]

Sth132I
CCCG
M.SuaII
RGATCY
2, 5
m5C
RGATCCCGNNNN[NNNN]

Sth132I
CCCG
M1.Bag18758II, M2.Bag18758II
CCCGTG
2, 4
Unknown
CCCGTGNN[NNNN]

Sth132I
CCCG
M1.BcnI, M2.BcnI, M.NciI
CCSGG
2, 4
m4C
CCCGGNNN[NNNN]

Sth1321
CCCG
M1.BspACI
CCGC
1
m5C
CCCGCNNN[NNNN]

Sth132I
CCCG
M1.CspNS6II
TCCGCC
5
m5C
TCCGCCCGNNNN[NNNN]

Alw26I, BcoDI,
GTCTC
M.AatII, M.Lsp6406III
GACGTC
2, 5
m6A
GACGTCTCN[NNNN]

BsmAI, BsoMAI,

BstMAI

Alw26I, BcoDI,
GTCTC
M.AbaBs12I, M.Awo1030I, M.Cdu23823I,
CCWGG
1, 5
m5C
CCWGGTCTCN[NNNN]

BsmAI, BsoMAI,

M.Csa8155II, M.Cun9529I, M.EasL1Dcm,

BstMAI

M.Eba57Dcm, M.Ecl34399Dcm,

M.Eco12761Dcm, M.Eco3609Dcm,

M.Eco4255Dcm, M.EcoVR50Dcm,

M.EsaSP1II, M.Kpn34618Dcm,

M.Kpn38547Dcm, M.Kpn39I, M.Kpn43816I,

M.Kpn677ORFDcm, M.Kpn928Dcm,

M.KpnNIH1Dcm, M.KpnNIH10Dcm,

M.KpnNIH30Dcm, M.Kva120Dcm, M.Lsp48I,

M.Pmi1I, M.SpaD2III, M.SplRp8II,

M.SptADcm, M.Tsu2489III

Alw26I, BcoDI,
GTCTC
M.AbrSp7I, M.Apa101655II, M.Ath21654I,
GANTC
2, 4
m6A
GAGTCTCN[NNNN]

BsmAI, BsoMAI,

M.BdiNK6I, M.Bel76I, M.Bsp460I,

BstMAI

M.BspJKG1II, M.BspLA1II, M.BspRAC04II,

M.CagII, M.CcrMI, M.CcrNAI, M.CnaPQ2I,

M.Csp16704III, M.CspNS6I, M.CviBI,

M.CviQVI, M.Eli8509I, M.Fnu419I, M.Fsp71I,

M.HbaI, M.HhaII, M.HinfI, M.HinHia1I,

M.Hpy298IV, M.Hpy299IV, M.Hpy32IV,

M.Hpy57IV, M.Hpy66IV, M.Hpy99IX,

M.HpyAIV, M.HpyFIV, M.HpyUM032IV,

M.HpyUM037IV, M.Mbo45III, M.Msp10I,

M.Msp73BI, M.MspAL11I, M.MspLW5II,

M.NhaXII, M.NpeUS61I, M.OspHL35I,

M.Pin17395II, M.Pla5III, M.PspLM6I,

M.RbaNRL2I, M.Rgi145II, M.Rmo1926I,

M.RpaIII, M.RsaII, M.RshIII, M.Rsp8I,

M.SmeC3I, M.SmoLV, M.Sna24252I,

M.SscL1I, M.SspM41II, M.SstE37II, M.ThaIII,

M.Xsp91I, M.ZmoCP4I

Alw26I, BcoDI,
GTCTC
M.AciI
GCGG
2, 4
m5C
GCGGTCTCN[NNNN]

BsmAI, BsoMAI,

BstMAI

Alw26I, BcoDI,
GTCTC
M.AflIII
ACRYGT
2, 5
m4C
ACRYGTCTCN[NNNN]

BsmAI, BsoMAI,

BstMAI

Alw26I, BcoDI,
GTCTC
M.AhdI
GACNNNN
2, 10
m6A
GACNNNNNGTCTCN[NNNN]

BsmAI, BsoMAI,

NGTC

BstMAI

Alw26I, BcoDI,
GTCTC
M.Ama55I, M.CnaPQ2III, M.Rmo1926II,
CGGCCG
1, 6
m5C
CGGCCGTCTCN[NNNN]

BsmAI, BsoMAI,

M.SenC1765IV, M.SspG1I, M.XorPXII

BstMAI

Alw26I, BcoDI,
GTCTC
M.AquI
CYCGRG
1, 6
m5C
CYCGRGTCTCN[NNNN]

BsmAI, BsoMAI,

BstMAI

Alw26I, BcoDI,
GTCTC
M.AvaIX, M.Cfr10I, M.Nme18VI, M.VchAI,
RCCGGY
2, 5
m5C
RCCGGTCTCN[NNNN]

BsmAI, BsoMAI,

M.Vch0395I

BstMAI

Alw26I, BcoDI,
GTCTC
M.BcoSlacII, M.MspLW5III, M.Nbr128I,
CTCGAG
1, 6
m4C
CTCGAGTCTCN[NNNN]

BsmAI, BsoMAI,

M.Psy18884II, M.Tam77409II, M.TbrGE1II,

BstMAI

M.TpaRLII

Alw26I, BcoDI,
GTCTC
M.BepI, M.FpsJV, M.Gel16401III, M.Pdi8503I
CGCG
1, 4
m5C
CGCGTCTCN[NNNN]

BsmAI, BsoMAI,

BstMAI

Alw26I, BcoDI,
GTCTC
M.BsoBI
CYCGRG
1, 6
m4C
CYCGRGTCTCN[NNNN]

BsmAI, BsoMAI,

BstMAI

Alw26I, BcoDI,
GTCTC
M.BsuFI, M.CpeAIV, M.MspI
CCGG
1, 4
m5C
CCGGTCTCN[NNNN]

BsmAI, BsoMAI,

BstMAI

Alw26I, BcoDI,
GTCTC
M.Cab13497I, M.Dac11109III, M.Dco13527I,
CTAG
1, 4
m4C
CTAGTCTCN[NNNN]

BsmAI, BsoMAI,

M.Djo10085I, M.Hbo11551I, M.Hka19301III,

BstMAI

M.Hme33500I, M.HsaR1I, M.Hsi33800I,

M.HspKM1WII, M.HspNII, M.HtuI, M.HvoDSI,

M.Lba2029II, M.Mbo45II, M.MjaI, M.MthZI,

M.Nas12278I, M.Rfl3010I, M.SsmMI

Alw26I, BcoDI,
GTCTC
M.CagVIII
ACGCGT
2, 5
m4C
ACGCGTCTCN[NNNN]

BsmAI, BsoMAI,

BstMAI

Alw26I, BcoDI,
GTCTC
M.CfrBI, M.Sen1735IV, M.SenAnaIV
CCWWGG
1, 6
m4C
CCWWGGTCTCN[NNNN]

BsmAI, BsoMAI,

BstMAI

Alw26I, BcoDI,
GTCTC
M.Cmu51329I, M.EcoJA03PDcm,
CCWGG
1, 5
Unknown
CCWGGTCTCN[NNNN]

BsmAI, BsoMAI,

M.RorR1Dcm, M.Sen8391 Dcm, M.ZalSM2II

BstMAI

Alw26I, BcoDI,
GTCTC
M.DdeI
CTNAG
1, 5
m5C
CTNAGTCTCN[NNNN]

BsmAI, BsoMAI,

BstMAI

Alw26I, BcoDI,
GTCTC
M.Esp3I
CGTCTC
4, 3
m5C,

BsmAI, BsoMAI,

m6A

BstMAI

Alw26I, BcoDI,
GTCTC
M.FatI
CATG
1, 4
m5C
CATGTCTCN[NNNN]

BsmAI, BsoMAI,

BstMAI

Alw26I, BcoDI,
GTCTC
M.FisAW1I, M.ThaI, M.Tph12270II
CGCG
1, 4
m4C
CGCGTCTCN[NNNN]

BsmAI, BsoMAI,

BstMAI

Alw26I, BcoDI,
GTCTC
M.FnoB1II, M.Hal24586I, M.Hpy298V,
CCGG
1, 4
m4C
CCGGTCTCN[NNNN]

BsmAI, BsoMAI,

M.Hpy299IX, M.Hpy30V, M.Hpy32XI,

BstMAI

M.Hpy57V, M.Hpy66IX, M.Hpy99VIII,

M.HpyFV, M.HpyUM032IX, M.HpyUM037IX,

M.MjaVI, M.PspJDRI, M.TmeBII

Alw26I, BcoDI,
GTCTC
M.GsuRed1IV, M.Osp807II, M.Tth111I
GACNNNG
2, 8
m6A
GACNNNGTCTCN[NNNN]

BsmAI, BsoMAI,

TC

BstMAI

Alw26I, BcoDI,
GTCTC
M.Hpy298II, M.Hpy299II, M.Hpy66II,
ACNGT
2, 4
m4C
ACNGTCTCN[NNNN]

BsmAI, BsoMAI,

M.HpyUM032II, M.HpyUM037II

BstMAI

Alw26I, BcoDI,
GTCTC
M.Hpy30X, M.Hpy57VIII, M.HpyFXI, M.MhuI
CTNAG
1, 5
m4C
CTNAGTCTCN[NNNN]

BsmAI, BsoMAI,

BstMAI

Alw26I, BcoDI,
GTCTC
M.Hpy99IV, M.HpyFORFX
CCNNGG
1, 6
m4C
CCNNGGTCTCN[NNNN]

BsmAI, BsoMAI,

BstMAI

Alw26I, BcoDI,
GTCTC
M.Hpy99XI
ACGT
2, 3
m5C
ACGTCTCN[NNNN]

BsmAI, BsoMAI,

BstMAI

Alw26I, BcoDI,
GTCTC
M.HpyD27I
GAGG
2, 4
m6A,
GAGGTCTCN[NNNN]

BsmAI, BsoMAI,

m5C

BstMAI

Alw26I, BcoDI,
GTCTC
M.Lph145I, M.Lph93I
TCWCC
1
m6A
GTCTCC[NNNN]

BsmAI, BsoMAI,

BstMAI

Alw26I, BcoDI,
GTCTC
M.MlyI, M.Pen113I
GASTC
2, 4
m6A
GAGTCTCN[NNNN]

BsmAI, BsoMAI,

BstMAI

Alw26I, BcoDI,
GTCTC
M.Msp1II
CCAGG
5
m4C
CCAGGTCTCN[NNNN]

BsmAI, BsoMAI,

BstMAI

Alw26I, BcoDI,
GTCTC
M.Msp42II
CCWGG
1, 5
m4C
CCWGGTCTCN[NNNN]

BsmAI, BsoMAI,

BstMAI

Alw26I, BcoDI,
GTCTC
M.NmeDI
VCCGGY
2, 5
m5C
VCCGGTCTCN[NNNN]

BsmAI, BsoMAI,

BstMAI

Alw26I, BcoDI,
GTCTC
M.NmeMC58III
TCTGG
5
m5C
TCTGGTCTCN[NNNN]

BsmAI, BsoMAI,

BstMAI

Alw26I, BcoDI,
GTCTC
M.Nsp209III, M.Nsp51109II
CTCGAG
1, 6
m5C
CTCGAGTCTCN[NNNN]

BsmAI, BsoMAI,

BstMAI

Alw26I, BcoDI,
GTCTC
M.NspI, M.NspHI
RCATGY
2, 5
m5C
RCATGTCTCN[NNNN]

BsmAI, BsoMAI,

BstMAI

Alw26I, BcoDI,
GTCTC
M.Pam7686I
CCATGG
1, 6
m5C
CCATGGTCTCN[NNNN]

BsmAI, BsoMAI,

BstMAI

Alw26I, BcoDI,
GTCTC
M.Pfa23572II
CGATCG
1, 6
m5C
CGATCGTCTCN[NNNN]

BsmAI, BsoMAI,

BstMAI

Alw26I, BcoDI,
GTCTC
M.Pla5I
CTAG
1, 4
Unknown
CTAGTCTCN[NNNN]

BsmAI, BsoMAI,

BstMAI

Alw26I, BcoDI,
GTCTC
M.Sam53668II
CCATGG
1, 6
Unknown
CCATGGTCTCN[NNNN]

BsmAI, BsoMAI,

BstMAI

Alw26I, BcoDI,
GTCTC
M.Sen1781III
CCWWGG
1, 6
Unknown
CCWWGGTCTCN[NNNN]

BsmAI, BsoMAI,

BstMAI

Alw26I, BcoDI,
GTCTC
M.SonIII
GCANNNN
3, 9
m6A

BsmAI, BsoMAI,

GTC

BstMAI

Alw26I, BcoDI,
GTCTC
M.TdeII
GAAGAG
5, 6
m6A,
GAAGAGTCTCN[NNNN]

BsmAI, BsoMAI,

m4C

BstMAI

Alw26I, BcoDI,
GTCTC
M.TspRI
CASTG
1, 5
m5C
CASTGTCTCN[NNNN]

BsmAI, BsoMAI,

BstMAI

Alw26I, BcoDI,
GTCTC
M1.AgoG2I, M2.AgoG2I
CCAGT
1, 4
m4C
ACTGGTCTCN[NNNN],

BsmAI, BsoMAI,

CCAGTCTCN[NNNN]

BstMAI

Alw26I, BcoDI,
GTCTC
M1.BceSIII
GCCGT
4
m4C
GCCGTCTCN[NNNN]

BsmAI, BsoMAI,

BstMAI

Alw26I, BcoDI,
GTCTC
M1.BspACI
GCGG
4
m5C
GCGGTCTCN[NNNN]

BsmAI, BsoMAI,

BstMAI

Alw26I, BcoDI,
GTCTC
M1.Dsh12II, M2.Dsh12II
GCCGTC
3, 4
m4C
GCCGTCTCN[NNNN]

BsmAI, BsoMAI,

BstMAI

Alw26I, BcoDI,
GTCTC
M1.Eco31I, M1.EcoMI
GGTCTC
3
m6A
GGTCTCN[NNNN]

BsmAI, BsoMAI,

BstMAI

Alw26I, BcoDI,
GTCTC
M1.Hpy32II
TCYYC
1
m6A
GTCTCC[NNNN]

BsmAI, BsoMAI,

BstMAI

Alw26I, BcoDI,
GTCTC
M1.LplAG30I
GAAGG
5
m5C
GAAGGTCTCN[NNNN]

BsmAI, BsoMAI,

BstMAI

Alw26I, BcoDI,
GTCTC
M2.BceSIII, M1.Pru9I, M2.Pru9I
GCCGT
2, 4
m4C
GCCGTCTCN[NNNN]

BsmAI, BsoMAI,

BstMAI

Alw26I, BcoDI,
GTCTC
M2.BfiI
CCCAGT
5
m4C
CCCAGTCTCN[NNNN]

BsmAI, BsoMAI,

BstMAI

Alw26I, BcoDI,
GTCTC
M2.BfuAI
GCAGGT
5
m5C
GCAGGTCTCN[NNNN]

BsmAI, BsoMAI,

BstMAI

Alw26I, BcoDI,
GTCTC
M2.Eco31I
GGTCTC
4
m5C
GGTCTCN[NNNN]

BsmAI, BsoMAI,

BstMAI

Alw26I, BcoDI,
GTCTC
M2.HgaI, M1.LlaJI
GCGTC
3
m5C
GCGTCTCN[NNNN]

BsmAI, BsoMAI,

BstMAI

Alw26I, BcoDI,
GTCTC
M2.HpyAVI, M1.MnlI
GAGG
4
m5C
GAGGTCTCN[NNNN]

BsmAI, BsoMAI,

BstMAI

Alw26I, BcoDI,
GTCTC
M2.Nme579IV, M2.NmeMC58II
GCGTC
4
m6A
GCGTCTCN[NNNN]

BsmAI, BsoMAI,

BstMAI

AlwXI, BbvI,
GCAG
M.AbaBs12I, M.Awo1030I, M.Cdu23823I,
CCWGG
1, 5
m5C
CCWGGCAGCNNNNNNNN[NNNN],

BseKI, BseXI,
C
M.Csa8155II, M.Cun9529I, M.EasL1Dcm,

GCAGCCWGGNNNN[NNNN]

Bsp423I, Bst12I,

M.Eba57Dcm, M.Ecl34399Dcm,

Bst71I, BstV1I,

M.Eco12761Dcm, M.Eco3609Dcm,

GeoICI, Lsp1109I

M.Eco4255Dcm, M.EcoVR50Dcm,

M.EsaSP1II, M.Kpn34618Dcm,

M.Kpn38547Dcm, M.Kpn39I, M.Kpn43816I,

M.Kpn677ORFDcm, M.Kpn928Dcm,

M.KpnNIH1Dcm, M.KpnNIH10Dcm,

M.KpnNIH30Dcm, M.Kva120Dcm, M.Lsp48I,

M.Pmi1I, M.SpaD2III, M.SplRp8II,

M.SptADcm, M.Tsu2489III

AlwXI, BbvI,
GCAG
M.Acc65VI, M.SenAZIII
CTKMAG
2, 5
m6A
CTGCAGCNNNNNNNN[NNNN]

BseKI, BseXI,
C

Bsp423I, Bst12I,

Bst71I, BstV1I,

GeoICI, Lsp1109I

AlwXI, BbvI,
GCAG
M.AciI
CCGC
1, 3
m5C
GCGGCAGCNNNNNNNN[NNNN],

BseKI, BseXI,
C

GCAGCGGNNNNNN[NNNN],

Bsp423I, Bst12I,

CCGCAGCNNNNNNNN[NNNN],

Bst71I, BstV1I,

GCAGCCGCNNNNN[NNNN]

GeoICI, Lsp1109I

AlwXI, BbvI,
GCAG
M.Ade56712I, M.Asp588III, M.AveCB51II,
CTGCAG
2, 5
m6A
CTGCAGCNNNNNNNN[NNNN]

BseKI, BseXI,
C
M.BbrUIII, M.Blo30698I, M.BsuBI,

Bsp423I, Bst12I,

M.CpaYL7III, M.Dba7044I, M.Dfa45958II,

Bst71I, BstV1I,

M.Eco12581I, M.Eco2012I, M.EcoGIII,

GeoICI, Lsp1109I

M.EcoG089I, M.Eho16691II, M.Hal5920II,

M.Lsp6406II, M.MspNR41II, M.Pae490I,

M.Pox23570I, M.PstI, M.RpaII, M.Sca3403II,

M.Tsp61I, M.XmnII, M.YenWI, M.YinY228I

AlwXI, BbvI,
GCAG
M.AluBI
AGCT
1, 4
m6A
GCAGCTNNNNNNN[NNNN]

BseKI, BseXI,
C

Bsp423I, Bst12I,

Bst71I, BstV1I,

GeoICI, Lsp1109I

AlwXI, BbvI,
GCAG
M.AluI, M.CspCY2I
AGCT
2, 3
m5C
GCAGCTNNNNNNN[NNNN]

BseKI, BseXI,
C

Bsp423I, Bst12I,

Bst71I, BstV1I,

GeoICI, Lsp1109I

AlwXI, BbvI,
GCAG
M.Ama55I, M.CnaPQ2III, M.Rmo1926II,
CGGCCG
1, 6
m5C
CGGCCGCAGCNNNNNNNN[NNNN],

BseKI, BseXI,
C
M.SenC1765IV, M.SspG1I, M.XorPXII

GCAGCGGCCGNNN[NNNN]

Bsp423I, Bst12I,

Bst71I, BstV1I,

GeoICI, Lsp1109I

AlwXI, BbvI,
GCAG
M.AplI
CTGCAG
3, 4
m5C
CTGCAGCNNNNNNNN[NNNN]

BseKI, BseXI,
C

Bsp423I, Bst12I,

Bst71I, BstV1I,

GeoICI, Lsp1109I

AlwXI, BbvI,
GCAG
M.AquI
CYCGRG
1, 6
m5C
CYCGRGCAGCNNNNNNNN[NNNN],

BseKI, BseXI,
C

GCAGCYCGRGNNN[NNNN]

Bsp423I, Bst12I,

Bst71I, BstV1I,

GeoICI, Lsp1109I

AlwXI, BbvI,
GCAG
M.AsiSI
GCGATCGC
2, 7
m5C
GCGATCGCAGCNNNNNNNN[NNNN],

BseKI, BseXI,
C

GCAGCGATCGCNN[NNNN]

Bsp423I, Bst12I,

Bst71I, BstV1I,

GeoICI, Lsp1109I

AlwXI, BbvI,
GCAG
M.AvaIX, M.Cfr10I, M.Nme18VI, M.VchAI,
RCCGGY
2, 5
m5C
RCCGGCAGCNNNNNNNN[NNNN],

BseKI, BseXI,
C
M.Vch0395I

GCAGCCGGYNNNN[NNNN]

Bsp423I, Bst12I,

Bst71I, BstV1I,

GeoICI, Lsp1109I

AlwXI, BbvI,
GCAG
M.BcoSlacII, M.MspLW5III, M.Nbr128I,
CTCGAG
1, 6
m4C
CTCGAGCAGCNNNNNNNN[NNNN],

BseKI, BseXI,
C
M.Psy18884II, M.Tam77409II, M.TbrGE1II,

GCAGCTCGAGNNN[NNNN]

Bsp423I, Bst12I,

M.TpaRLII

Bst71I, BstV1I,

GeoICI, Lsp1109I

AlwXI, BbvI,
GCAG
M.BepI, M.FpsJV, M.Gel16401III, M.Pdi8503I
CGCG
1, 4
m5C
CGCGCAGCNNNNNNNN[NNNN],

BseKI, BseXI,
C

GCAGCGCGNNNNN[NNNN]

Bsp423I, Bst12I,

Bst71I, BstV1I,

GeoICI, Lsp1109I

AlwXI, BbvI
GCAG
M.BglI, M.CagI, M.DdeVCDI, M.GsuRed1I
GCCNNNN
2, 10
m4C
GCCNNNNNGGCAGCNNNNNNNN[NNNN]

BseKI, BseXI,
C

NGGC

Bsp423I, Bst12I,

Bst71I BstV1I,

GeoICI, Lsp1109I

AlwXI, BbvI,
GCAG
M.Ble402III, M.Psp10HI
SAGCTS
3, 4
m4C
GCAGCTSNNNNNN[NNNN]

BseKI, BseXI,
C

Bsp423I, Bst12I,

Bst71I, BstV1I,

GeoICI, Lsp1109I

AlwXI, BbvI,
GCAG
M.BsoBI
CYCGRG
1, 6
m4C
CYCGRGCAGCNNNNNNNN[NNNN],

BseKI, BseXI,
C

GCAGCYCGRGNNN[NNNN]

Bsp423I, Bst12I,

Bst71I, BstV1I,

GeoICI, Lsp11091

AlwXI, BbvI,
GCAG
M.BsuFI, M.CpeAIV, M.MspI
CCGG
1, 4
m5C
CCGGCAGCNNNNNNNN[NNNN],

BseKI, BseXI,
C

GCAGCCGGNNNNN[NNNN]

Bsp423I, Bst12I,

Bst71I, BstV1I,

GeoICI, Lsp1109I

AlwXI, BbvI,
GCAG
M.Cab13497I, M.Dac11109III, M.Dco13527I,
CTAG
1, 4
m4C
CTAGCAGCNNNNNNNN[NNNN],

BseKI, BseXI,
C
M.Djo10085I, M.Hbo11551I, M.Hka19301III,

GCAGCTAGNNNNN[NNNN]

Bsp423I, Bst12I,

M.Hme33500I, M.HsaR1I, M.Hsi33800I,

Bst71I, BstV1I,

M.HspKM1WII, M.HspNII, M.HtuI, M.HvoDSI,

GeoICI, Lsp1109I

M.Lba2029II, M.Mbo45II, M.MjaI, M.MthZI,

M.Nas12278I, M.Rfl3010I, M.SsmMI

AlwXI, BbvI,
GCAG
M.Cac8I
GCNNGC
2, 5
m5C
GCNNGCAGCNNNNNNNN[NNNN],

BseKI, BseXI,
C

GCAGCNNGCNNNN[NNNN]

Bsp423I, Bst12I,

Bst71I, BstV1I,

GeoICI, Lsp1109I

AlwXI, BbvI
GCAG
M.CcrNAV
YGCCGGCR
3, 6
m5C
YGCCGGCAGCNNNNNNNN[NNNN]

BseKI, BseXI,
C

Bsp423I, Bst12I,

Bst71I, BstV1I,

GeoICI, Lsp1109I

AlwXI, BbvI
GCAG
M.CfrBI, M.Sen1735IV, M.SenAnaIV
CCWWGG
1, 6
m4C
CCWWGGCAGCNNNNNNNN[NNNN],

BseKI, BseXI,
C

GCAGCCWWGGNNN[NNNN]

Bsp423I, Bst12I,

Bst71I, BstV1I,

GeoICI, Lsp1109I

AlwXI, BbvI,
GCAG
M.Cmu51329I, M.EcoJA03PDcm,
CCWGG
1, 5
Unknown
CCWGGCAGCNNNNNNNN[NNNN],

BseKI, BseXI,
C
M.RorR1Dcm, M.Sen8391Dcm, M.ZalSM2II

GCAGCCWGGNNNN[NNNN]

Bsp423I, Bst12I,

Bst71I, BstV1I,

GeoICI, Lsp1109I

AlwXI, BbvI,
GCAG
M.CsiFSMII, M.MfoCT6IV, M.NgoAIV,
GCCGGC
2, 5
m5C
GCCGGCAGCNNNNNNNN[NNNN],

BseKI, BseXI,
C
M.NgoMIV, M1.Sgr13350II

GCAGCCGGCNNNN[NNNN]

Bsp423I, Bst12I,

Bst71I, BstV1I,

GeoICI, Lsp1109I

AlwXI, BbvI,
GCAG
M.CviJI
RGCB
2, 3
m5C
GCAGCBNNNNNNN[NNNN]

BseKI, BseXI,
C

Bsp423I, Bst12I,

Bst71I, BstV1I,

GeoICI, Lsp1109I

AlwXI, BbvI,
GCAG
M.CviRI, M.Hpy298VIII, M.Hpy299VIII,
TGCA
1, 4
m6A
TGCAGCNNNNNNNN[NNNN]

BseKI, BseXI,
C
M.Hpy30VI, M.Hpy32V, M.Hpy57II,

Bsp423I, Bst12I,

M.Hpy66VIII, M.HpyFVI, M.HpyUM032VIII,

Bst71I, BstV1I,

M.HpyUM037VIII

GeoICI, Lsp1109I

AlwXI, BbvI,
GCAG
M.Ddel
CTNAG
1, 5
m5C
CTNAGCAGCNNNNNNNN[NNNN],

BseKI, BseXI,
C

GCAGCTNAGNNNN[NNNN]

Bsp423I, Bst12I,

Bst71I, BstV1I,

GeoICI, Lsp1109I

AlwXI, BbvI,
GCAG
M.DfeI, M.Fnu419II, M.Pen113II
CTNNAG
2, 5
m6A
CTGCAGCNNNNNNNN[NNNN]

BseKI, BseXI,
C

Bsp423I, Bst12I,

Bst71I, BstV1I,

GeoICI, Lsp1109I

AlwXI, BbvI,
GCAG
M.FatI
CATG
1, 4
m5C
CATGCAGCNNNNNNNN[NNNN],

BseKI, BseXI,
C

GCAGCATGNNNNN[NNNN]

Bsp423I, Bst12I,

Bst71I, BstV1I,

GeoICI, Lsp1109I

AlwXI, BbvI,
GCAG
M.FisAW1I, M.ThaI, M.Tph12270II
CGCG
1, 4
m4C
CGCGCAGCNNNNNNNN[NNNN],

BseKI, BseXI,
C

GCAGCGCGNNNNN[NNNN]

Bsp423I, Bst12I,

Bst71I, BstV1I,

GeoICI, Lsp1109I

AlwXI, BbvI,
GCAG
M.FnoB1II, M.Hal24586I, M.Hpy298V,
CCGG
1, 4
m4C
CCGGCAGCNNNNNNNN[NNNN],

BseKI, BseXI,
C
M.Hpy299IX, M.Hpy30V, M.Hpy32XI,

GCAGCCGGNNNNN[NNNN]

Bsp423I, Bst12I,

M.Hpy57V, M.Hpy66IX, M.Hpy99VIII,

Bst71I, BstV1I,

M.HpyFV, M.HpyUM032IX, M.HpyUM037IX,

GeoICI, Lsp1109I

M.MjaVI, M.PspJDRI, M.TmeBII

AlwXI, BbvI,
GCAG
M.Fsi4947I, M.Mru1279IV, M.NsoJS138I,
CAGCTG
3, 4
m4C
GCAGCTGNNNNNN[NNNN]

BseKI, BseXI,
C
M1.Psp320WII, M.PvuII, M.SenA46IV,

Bsp423I, Bst12I,

M.SptAI

Bst71I, BstV1I,

GeoICI, Lsp1109I

AlwXI, BbvI,
GCAG
M.HhaI, M.HinP1I, M.Hpy99III, M.HpyAVIII,
GCGC
2, 3
m5C
GCGCAGCNNNNNNNN[NNNN],

BseKI, BseXI,
C
M.HpyUM032XV

GCAGCGCNNNNNN[NNNN]

Bsp423I, Bst12I,

Bst71I, BstV1I,

GeoICI, Lsp11091

AlwXI, BbvI,
GCAG
M.Hme33500II
HGCWGCK
3
m4C
MGCWGCAGCNNNNNNNN[NNNN],

BseKI, BseXI,
C

MGCAGCDNNNNNNN[NNNN],

Bsp423I, Bst12I,

HGCAGCKNNNNNNN[NNNN],

Bst71I, BstV1I,

GCAGCWGCKNNNN[NNNN]

GeoICI, Lsp11091

AlwXI, BbvI,
GCAG
M.Hpy30X, M.Hpy57VIII, M.HpyFXI, M.MhuI
CTNAG
1, 5
m4C
CTNAGCAGCNNNNNNNN[NNNN],

BseKI, BseXI,
C

GCAGCTNAGNNNN[NNNN]

Bsp423I, Bst12I,

Bst71I, BstV1I,

GeoICI, Lsp1109I

AlwXI, BbvI,
GCAG
M.Hpy57X
CTRYAG
2, 5
m6A
CTGCAGCNNNNNNNN[NNNN]

BseKI, BseXI,
C

Bsp423I, Bst12I,

Bst71I, BstV1I,

GeoICI, Lsp1109I

AlwXI, BbvI,
GCAG
M.Hpy99IV, M.HpyFORFX
CCNNGG
1, 6
m4C
CCNNGGCAGCNNNNNNNN[NNNN],

BseKI, BseXI,
C

GCAGCCNNGGNNN[NNNN]

Bsp423I, Bst12I,

Bst71I, BstV1I,

GeoICI, Lsp1109I

AlwXI, BbvI,
GCAG
M.HpyD27I
GAGG
2, 4
m6A,
GCAGCCTCNNNNN[NNNN],

BseKI, BseXI,
C

m5C
GAGGCAGCNNNNNNNN[NNNN]

Bsp423I, Bst12I,

Bst71I, BstV1I,

GeoICI, Lsp1109I

AlwXI, BbvI,
GCAG
M.Mma5219II, M.TarMY3I
AGCT
2, 3
m4C
GCAGCTNNNNNNN[NNNN]

BseKI, BseXI,
C

Bsp423I, Bst12I,

Bst71I, BstV1I,

GeoICI, Lsp1109I

AlwXI, BbvI,
GCAG
M.MpeII
RGCY
2, 3
m5C
GCAGCYNNNNNNN[NNNN]

BseKI, BseXI,
C

Bsp423I, Bst12I,

Bst71I, BstV1I,

GeoICI, Lsp1109I

AlwXI, BbvI,
GCAG
M.Msp1II
CCAGG
5
m4C
GCAGCCTGGNNNN[NNNN],

BseKI, BseXI,
C

CCAGGCAGCNNNNNNNN[NNNN]

Bsp423I, Bst12I,

Bst71I, BstV1I,

GeoICI, Lsp1109I

AlwXI, BbvI,
GCAG
M.Msp42II
CCWGG
1, 5
m4C
CCWGGCAGCNNNNNNNN[NNNN],

BseKI, BseXI,
C

GCAGCCWGGNNNN[NNNN]

Bsp4231 Bst12I,

Bst71I, BstV1I,

GeoICI, Lsp1109I

AlwXI, BbvI,
GCAG
M.NgoAI
RGCGCY
3, 4
m5C
GCAGCGCYNNNNN[NNNN]

BseKI, BseXI,
C

Bsp423I, Bst12I,

Bst71I, BstV1I,

GeoICI, Lsp1109I

AlwXI, BbvI,
GCAG
M.NheI
GCTAGC
1, 6
m4C
GCTAGCAGCNNNNNNNN[NNNN],

BseKI, BseXI,
C

GCAGCTAGCNNNN[NNNN]

Bsp423I, Bst12I,

Bst71I, BstV1I,

GeoICI, Lsp1109I

AlwXI, BbvI,
GCAG
M.Nme18V
GCGCGC
2, 5
m5C
GCGCGCAGCNNNNNNNN[NNNN],

BseKI, BseXI,
C

GCAGCGCGCNNNN[NNNN]

Bsp423I, Bst12I,

Bst71I, BstV1I,

GeoICI, Lsp1109I

AlwXI, BbvI,
GCAG
M.NmeDI
VCCGGY
2, 5
m5C
VCCGGCAGCNNNNNNNN[NNNN],

BseKI, BseXI,
C

GCAGCCGGBNNNN[NNNN]

Bsp423I, Bst12I,

Bst71I, BstV1I

GeoICI, Lsp1109I

AlwXI, BbvI,
GCAG
M.NmeMC58III
CCAGA
1
m5C
TCTGGCAGCNNNNNNNN[NNNN],

BseKI, BseXI,
C

GCAGCCAGANNNN[NNNN]

Bsp423I, Bst12I,

Bst71I, BstV1I,

GeoICI, Lsp1109I

AlwXI, BbvI,
GCAG
M.Nsp209III, M.Nsp51109II
CTCGAG
1, 6
m5C
CTCGAGCAGCNNNNNNNN[NNNN],

BseKI, BseXI,
C

GCAGCTCGAGNNN[NNNN]

Bsp423I, Bst12I,

Bst71I, BstV1I,

GeoICI, Lsp11091

AlwXI, BbvI,
GCAG
M.NspI, M.NspHI
RCATGY
2, 5
m5C
RCATGCAGCNNNNNNNN[NNNN],

BseKI, BseXI,
C

GCAGCATGYNNNN[NNNN]

Bsp423I, Bst12I,

Bst71I, BstV1I,

GeoICI, Lsp1109I

AlwXI, BbvI,
GCAG
M.Pae10332II, M1.Pae50071I,
CAGCTC
3, 4
m4C
GCAGCTCNNNNNN[NNNN]

BseKI, BseXI,
C
M2.Pae50071I

Bsp423I, Bst12I,

Bst71I, BstV1I,

GeoICI, Lsp1109I

AlwXI, BbvI,
GCAG
M.Pam7686I
CCATGG
1, 6
m5C
CCATGGCAGCNNNNNNNN[NNNN],

BseKI, BseXI,
C

GCAGCCATGGNNN[NNNN]

Bsp423I, Bst12I,

Bst71I, BstV1I,

GeoICI, Lsp11091

AlwXI, BbvI,
GCAG
M.Pfa23572II
CGATCG
1, 6
m5C
CGATCGCAGCNNNNNNNN[NNNN],

BseKI, BseXI,
C

GCAGCGATCGNNN[NNNN]

Bsp423I, Bst12I,

Bst71I, BstV1I,

GeoICI, Lsp11091

AlwXI, BbvI,
GCAG
M.Pla5I
CTAG
1, 4
Unknown
CTAGCAGCNNNNNNNN[NNNN],

BseKI, BseXI,
C

GCAGCTAGNNNNN[NNNN]

Bsp423I, Bst12I,

Bst71I, BstV1I,

GeoICI, Lsp1109I

AlwXI, BbvI,
GCAG
M.Sam53668II
CCATGG
1, 6
Unknown
CCATGGCAGCNNNNNNNN[NNNN],

BseKI, BseXI,
C

GCAGCCATGGNNN[NNNN]

Bsp423I, Bst12I,

Bst71I, BstV1I,

GeoICI, Lsp1109I

AlwXI, BbvI,
GCAG
M.SbaUII
CAGCTG
2, 5
m6A
GCAGCTGNNNNNN[NNNN]

BseKI, BseXI,
C

Bsp423I, Bst12I,

Bst71I, BstV1I,

GeoICI, Lsp1109I

AlwXI, BbvI,
GCAG
M.Sen1781III
CCWWGG
1, 6
Unknown
CCWWGGCAGCNNNNNNNN[NNNN],

BseKI, BseXI,
C

GCAGCCWWGGNNN[NNNN]

Bsp423I Bst12I,

Bst71I, BstV1I,

GeoICI, Lsp1109I

AlwXI, BbvI,
GCAG
M.SonIII
GCANNNN
3, 9
m6A
GCAGCNNGTCNNN[NNNN]

BseKI, BseXI,
C

GTC

Bsp423I, Bst12I,

Bst71I, BstV1I,

GeoICI, Lsp11091

AlwXI, BbvI,
GCAG
M.TdeII
CTCTTC
1, 2
m4C,
GAAGAGCAGCNNNNNNNN[NNNN],

BseKI, BseXI,
C

m6A
GCAGCTCTTCNNN[NNNN]

Bsp423I, Bst12I,

Bst71I, BstV1I,

GeoICI, Lsp1109I

AlwXI, BbvI,
GCAG
M.TspRI
CASTG
1, 5
m5C
CASTGCAGCNNNNNNNN[NNNN],

BseKI, BseXI,
C

GCAGCASTGNNNN[NNNN]

Bsp423I, Bst12I,

Bst71I, BstV1I,

GeoICI, Lsp1109I

AlwXI, BbvI,
GCAG
M.Yen2516I
GCGCGC
2, 5
Unknown
GCGCGCAGCNNNNNNNN[NNNN],

BseKI, BseXI,
C

GCAGCGCGCNNNN[NNNN]

Bsp423I, Bst12I,

Bst71I, BstV1I,

GeoICI, Lsp11091

AlwXI, BbvI,
GCAG
M1.AgoG2I, M2.AgoG2I
CCAGT
1, 4
m4C
ACTGGCAGCNNNNNNNN[NNNN],

BseKI, BseXI,
C

GCAGCCAGTNNNN[NNNN]

Bsp423I, Bst12I,

Bst71I, BstV1I,

GeoICI, Lsp1109I

AlwXI, BbvI,
GCAG
M1.BspACI
CCGC
1
m5C
GCGGCAGCNNNNNNNN[NNNN],

BseKI, BseXI,
C

GCAGCCGCNNNNN[NNNN]

Bsp423I, Bst12I,

Bst71I, BstV1I,

GeoICI, Lsp1109I

AlwXI, BbvI,
GCAG
M1.HgaI, M2.LlaJI
GCGTC
2
m5C
GACGCAGCNNNNNNNN[NNNN],

BseKI, BseXI,
C

GCAGCGTCNNNNN[NNNN]

Bsp423I, Bst12I,

Bst71I, BstV1I,

GeoICI, Lsp1109I

AlwXI BbvI
GCAG
M1.LplAG30I
GAAGG
5
m5C
GCAGCCTTCNNNN[NNNN],

BseKI, BseXI,
C

GAAGGCAGCNNNNNNNN[NNNN]

Bsp423I, Bst12I,

Bst71I, BstV1I,

GeoICI, Lsp1109I

AlwXI, BbvI,
GCAG
M1.Sgr13350III, M2.Sgr13350III
GCTCTTC
2, 3
m4C,
GAAGAGCAGCNNNNNNNN[NNNN],

BseKI, BseXI,
C

m6A
GCAGCTCTTCNNN[NNNN]

Bsp423I, Bst12I,

Bst71I, BstV1I,

GeoICI, Lsp1109I

AlwXI, BbvI
GCAG
M2.BceSIII, M1.Pru9I, M2.Pru9I
ACGGC
2, 4
m4C
GCAGCCGTNNNNN[NNNN],

BseKI, BseXI,
C

ACGGCAGCNNNNNNNN[NNNN]

Bsp423I, Bst12I,

Bst71I, BstV1I,

GeoICI, Lsp1109I

AlwXI, BbvI,
GCAG
M2.HpyAVI, M1.MnlI
GAGG
4
m5C
GCAGCCTCNNNNN[NNNN],

BseKI, BseXI,
C

GAGGCAGCNNNNNNNN[NNNN]

Bsp423I, Bst12I,

Bst71I, BstV1I,

GeoICI, Lsp1109I

BmsI, BspST5I,
GCAT
M.AbaBs12I, M.Awo1030I, M.Cdu23823I,
CCWGG
1, 5
m5C
CCWGGCATCNNNNN[NNNN],

LweI, PhaI, SfaNI
C
M.Csa8155II, M.Cun9529I, M.EasL1Dcm,

GCATCCWGGN[NNNN]

M.Eba57Dcm, M.Ec134399Dcm,

M.Eco12761Dcm, M.Eco3609Dcm,

M.Eco4255Dcm, M.EcoVR50Dcm,

M.EsaSP1II, M.Kpn34618Dcm,

M.Kpn38547Dcm, M.Kpn39I, M.Kpn43816I,

M.Kpn677ORFDcm, M.Kpn928Dcm,

M.KpnNIH1Dcm, M.KpnNIH10Dcm,

M.KpnNIH30Dcm, M.Kva120Dcm, M.Lsp48I,

M.Pmi1I, M.SpaD2III, M.SplRp8II,

M.SptADcm, M.Tsu2489III

BmsI, BspST5I,
GCAT
M.AciI
CCGC
1, 3
m5C
GCGGCATCNNNNN[NNNN],

LweI, PhaI, SfaNI
C

CCGCATCNNNNN[NNNN],

GCATCCGCNN[NNNN]

BmsI, BspST5I,
GCAT
M.Ama55I, M.CnaPQ2III, M.Rmo1926II,
CGGCCG
1, 6
m5C
CGGCCGCATCNNNNN[NNNN],

LweI, PhaI, SfaNI
C
M.SenC1765IV, M.SspG1I, M.XorPXII

GCATCGGCCG[NNNN]

BmsI, BspST5I,
GCAT
M.AquI
CYCGRG
1, 6
m5C
CYCGRGCATCNNNNN[NNNN],

LweI, PhaI, SfaNI
C

GCATCYCGRG[NNNN]

BmsI, BspST5I,
GCAT
M.AsiSI
GCGATCGC
2, 7
m5C
GCGATCGCATCNNNNN[NNNN]

LweI, PhaI, SfaNI
C

BmsI, BspST5I,
GCAT
M.AvaIX, M.Cfr10I, M.Nme18VI, M.VchAI,
RCCGGY
2, 5
m5C
RCCGGCATCNNNNN[NNNN]

LweI, PhaI, SfaNI
C
M.Vch0395I

BmsI, BspST5I,
GCAT
M.BamWS8I, M.CthV, M.EfaRFI, M.Saf8902II,
GCWGC
2, 4
m5C
GCWGCATCNNNNN[NNNN]

LweI, PhaI, SfaNI
C
M.TacII, M.TcoKWC4III

BmsI, BspST5I,
GCAT
M.BbvI, M.BceSIV
GCAGC
2, 4
m5C
GCTGCATCNNNNN[NNNN],

LweI, PhaI, SfaNI
C

GCAGCATCNNNNN[NNNN]

BmsI, BspST5I,
GCAT
M.BcoSlacII, M.MspLW5III, M.Nbr128I,
CTCGAG
1, 6
m4C
CTCGAGCATCNNNNN[NNNN],

LweI, PhaI, SfaNI
C
M.Psy18884II, M.Tam77409II, M.TbrGE1II,

GCATCTCGAG[NNNN]

M.TpaRLII

BmsI, BspST5I,
GCAT
M.BepI, M.FpsJV, M.Gel16401III, M.Pdi8503I
CGCG
1, 4
m5C
CGCGCATCNNNNN[NNNN],

LweI, PhaI, SfaNI
C

GCATCGCGNN[NNNN]

BmsI, BspST5I,
GCAT
M.BfaSIII, M.Ccl10114I, M.CfrB38I,
ATGCAT
2, 5
m6A
ATGCATCNNNNN[NNNN]

LweI, PhaI, SfaNI
C
M.CfrH1I, M.EcoGVI, M.EcoKII,

M.Fnu10562II, M.SbaUIV, M.Sbo12419III,

M.Sbo268II, M.Sen10384II, M.Sen10708II,

M.Sen1080II, M.Sen1175III, M.Sen13IV,

M.Sen13311IV, M.Sen1387II, M.Sen1427III,

M.Sen14882III, M.Sen15791III, M.Sen158III,

M.Sen16I, M.Sen1655III, M.Sen1676III,

M.Sen1677III, M.Sen1728III, M.Sen1735III,

M.Sen1736IV, M.Sen1764III, M.Sen1766III,

M.Sen1781IV, M.Sen1783III, M.Sen18569II,

M.Sen1878III, M.Sen1880III, M.Sen1896III,

M.Sen1898III, M.Sen1899III, M.Sen1903III,

M.Sen1906III, M.Sen1908III, M.Sen1910III,

M.Sen1921III, M.Sen1927II, M.Sen195II,

M.Sen2050II, M.Sen2064III, M.Sen2069III,

M.Sen22462I, M.Sen255II, M.Sen318III,

M.Sen3387III, M.Sen3890III, M.Sen483III,

M.Sen624III, M.Sen641III, M.Sen7378II,

M.Sen8391III, M.Sen8692III, M.SenA46III,

M.SenAbaII, M.SenAboII, M.SenAnaIII,

M.SenC1736III, M.SenC1765III,

M.SenC1808IV, M.SenC1810III, M.SenJIII,

M.SenL1II, M.SenL15III, M.SenN1660II,

M.SenSARA26II, M.SenSPBIII, M.SenTFIII

BmsI, BspST5I,
GCAT
M.BglI, M.CagI, M.DdeVCDI, M.GsuRed1I
GCCNNNN
2, 10
m4C
GCCNNNNNGGCATCNNNNN[NNNN]

LweI, PhaI, SfaNI
C

NGGC

BmsI, BspST5I,
GCAT
M.BseCI, M.ClaI, M.MfoCT6III, M.Nfa11134I,
ATCGAT
2, 5
m6A
GCATCGATNN[NNNN]

LweI, PhaI, SfaNI
C
M.Nsp209I, M.Nsp51109I, M.Pfu17724II,

M.SonI

BmsI, BspST5I,
GCAT
M.BsoBI
CYCGRG
1, 6
m4C
CYCGRGCATCNNNNN[NNNN],

LweI, PhaI, SfaNI
C

GCATCYCGRG[NNNN]

BmsI, BspST5I,
GCAT
M.BsuFI, M.CpeAIV, M.MspI
CCGG
1, 4
m5C
CCGGCATCNNNNN[NNNN],

LweI, PhaI, SfaNI
C

GCATCCGGNN[NNNN]

BmsI, BspST5I,
GCAT
M.Cab13497I, M.Dac11109III, M.Dco13527I,
CTAG
1, 4
m4C
CTAGCATCNNNNN[NNNN],

LweI, PhaI, SfaNI
C
M.Djo10085I, M.Hbo11551I, M.Hka19301III,

GCATCTAGNN[NNNN]

M.Hme33500I, M.HsaR1I, M.Hsi33800I,

M.Hsp KM1WII, M.HspNII, M.HtuI, M.HvoDSI,

M.Lba2029II, M.Mbo45II, M.MjaI, M.MthZI,

M.Nas12278I, M.Rfl3010I, M.SsmMI

BmsI, BspST5I,
GCAT
M.Cac8I
GCNNGC
2, 5
m5C
GCNNGCATCNNNNN[NNNN]

LweI, PhaI, SfaNI
C

BmsI, BspST5I,
GCAT
M.CagVI
TCCGGA
1, 6
m6A
GCATCCGGAN[NNNN]

LweI, PhaI, SfaNI
C

BmsI, BspST5I,
GCAT
M.CcrNAV
YGCCGGCR
3, 6
m5C
YGCCGGCATCNNNNN[NNNN]

LweI, PhaI, SfaNI
C

BmsI, BspST5I,
GCAT
M.Cdi630IV, M.Ckr177III, M.CmaLM2II,
GCNGC
2, 4
m5C
GCNGCATCNNNNN[NNNN]

LweI, PhaI, SfaNI
C
M.CocII, M.Fnu4HI, M.Fsp4HI

BmsI, BspST5I,
GCAT
M.CfrBI, M.Sen1735IV, M.SenAnaIV
CCWWGG
1, 6
m4C
CCWWGGCATCNNNNN[NNNN],

LweI, PhaI, SfaNI
C

GCATCCWWGG[NNNN]

BmsI, BspST5I,
GCAT
M.Cmu51329I, M.EcoJA03PDcm,
CCWGG
1, 5
Unknown
CCWGGCATCNNNNN[NNNN],

LweI, PhaI, SfaNI
C
M.RorR1Dcm, M.Sen8391 Dcm, M.ZalSM2II

GCATCCWGGN[NNNN]

BmsI, BspST5I,
GCAT
M.CsiFSMII, M.MfoCT6IV, M.NgoAIV,
GCCGGC
2, 5
m5C
GCCGGCATCNNNNN[NNNN]

LweI, PhaI, SfaNI
C
M.NgoMIV, M1.Sgr13350II

BmsI, BspST5I,
GCAT
M.CviQIII, M.CviSIII, M.Hpy299XI,
TCGA
1, 4
m6A
GCATCGANNN[NNNN]

LweI, PhaI, SfaNI
C
M.Hpy30II, M.Hpy32X, M.Hpy99VII,

M.HpyAX, M.HpyFII, M.HpyUM032XVII,

M.Tam77409III, M.TaqI, M.TpaRLI,

M.Tth HB8I

BmsI, BspST5I,
GCAT
M.CviRI, M.Hpy298VIII, M.Hpy299VIII,
TGCA
1, 4
m6A
TGCATCNNNNN[NNNN]

LweI, PhaI, SfaNI
C
M.Hpy30VI, M.Hpy32V, M.Hpy57II,

M.Hpy66VIII, M.HpyFVI, M.HpyUM032VIII,

M.HpyUM037VIII

BmsI, BspST5I,
GCAT
M.Ddel
CTNAG
1, 5
m5C
CTNAGCATCNNNNN[NNNN],

LweI, PhaI, SfaNI
C

GCATCTNAGN[NNNN]

BmsI, BspST5I,
GCAT
M.FatI
CATG
1, 4
m5C
CATGCATCNNNNN[NNNN],

LweI, PhaI, SfaNI
C

GCATCATGNN[NNNN]

BmsI, BspST5I,
GCAT
M.FisAW1I, M.ThaI, M.Tph12270II
CGCG
1, 4
m4C
CGCGCATCNNNNN[NNNN],

LweI, PhaI, SfaNI
C

GCATCGCGNN[NNNN]

BmsI, BspST5I,
GCAT
M.FnoB1II, M.Hal24586I, M.Hpy298V,
CCGG
1, 4
m4C
CCGGCATCNNNNN[NNNN],

LweI, PhaI, SfaNI
C
M.Hpy299IX, M.Hpy30V, M.Hpy32XI,

GCATCCGGNN[NNNN]

M.Hpy57V, M.Hpy66IX, M.Hpy99VIII,

M.HpyFV, M.HpyUM032IX, M.HpyUM037IX,

M.MjaVI, M.PspJDRI, M.TmeBII

BmsI, BspST5I,
GCAT
M.HhaI, M.HinP1I, M.Hpy99III, M.HpyAVIII,
GCGC
2, 3
m5C
GCGCATCNNNNN[NNNN]

LweI, PhaI, SfaNI
C
M.HpyUM032XV

BmsI, BspST5I,
GCAT
M.Hka19301IV
TCGCGA
2, 5
m4C
GCATCGCGAN[NNNN]

LweI, PhaI, SfaNI
C

BmsI, BspST5I,
GCAT
M.Hme33500II
MGCWGCD
5
m4C
MGCWGCATCNNNNN[NNNN]

LweI, PhaI, SfaNI
C

BmsI, BspST5I,
GCAT
M.Hpy188I, M.Hpy298IX, M.Hpy299V,
TCNGA
1, 5
m6A
GCATCNGANN[NNNN]

LweI, PhaI, SfaNI
C
M.Hpy66V, M.HpyUM032V

BmsI, BspST5I,
GCAT
M.Hpy30VIII, M.Hpy32VIII, M.Hpy57III,
TCNNGA
1, 6
m6A
GCATCNNGAN[NNNN]

LweI, PhaI, SfaNI
C
M.Hpy66VI, M.Hpy99XVIII, M.HpyFVIII,

M.HpyPVIII

BmsI, BspST5I,
GCAT
M.Hpy30X, M.Hpy57VIII, M.HpyFXI, M.MhuI
CTNAG
1, 5
m4C
CTNAGCATCNNNNN[NNNN],

LweI, PhaI, SfaNI
C

GCATCTNAGN[NNNN]

BmsI, BspST5I,
GCAT
M.Hpy99IV, M.HpyFORFX
CCNNGG
1, 6
m4C
CCNNGGCATCNNNNN[NNNN],

LweI, PhaI, SfaNI
C

GCATCCNNGG[NNNN]

BmsI, BspST5I,
GCAT
M.HpyD27I
GAGG
2, 4
m6A,
GCATCCTCNN[NNNN],

LweI, PhaI, SfaNI
C

m5C
GAGGCATCNNNNN[NNNN]

BmsI, BspST5I,
GCAT
M.Lph145I, M.Lph93I
TCWCC
1
m6A
GCATCWCCNN[NNNN]

LweI, PhaI, SfaNI
C

BmsI, BspST5I,
GCAT
M.Msp1II
CCAGG
5
m4C
GCATCCTGGN[NNNN],

LweI, PhaI, SfaNI
C

CCAGGCATCNNNNN[NNNN]

BmsI, BspST5I,
GCAT
M.Msp42II
CCWGG
1, 5
m4C
CCWGGCATCNNNNN[NNNN],

LweI, PhaI, SfaNI
C

GCATCCWGGN[NNNN]

BmsI, BspST5I,
GCAT
M.NheI
GCTAGC
1, 6
m4C
GCTAGCATCNNNNN[NNNN]

LweI, PhaI, SfaNI
C

BmsI, BspST5I,
GCAT
M.Nme18III
TCTGG
2, 4
m5C
GCATCTGGNN[NNNN]

LweI, PhaI, SfaNI
C

BmsI, BspST5I,
GCAT
M.Nme18V
GCGCGC
2, 5
m5C
GCGCGCATCNNNNN[NNNN]

LweI, PhaI, SfaNI
C

BmsI, BspST5I,
GCAT
M.NmeDI
VCCGGY
2, 5
m5C
VCCGGCATCNNNNN[NNNN]

LweI, PhaI, SfaNI
C

BmsI, BspST5I,
GCAT
M.NmeMC58III
CCAGA
1
m5C
TCTGGCATCNNNNN[NNNN],

LweI, PhaI, SfaNI
C

GCATCCAGAN[NNNN]

BmsI, BspST5I,
GCAT
M.Nsp209III, M.Nsp51109II
CTCGAG
1, 6
m5C
CTCGAGCATCNNNNN[NNNN],

LweI, PhaI, SfaNI
C

GCATCTCGAG[NNNN]

BmsI, BspST5I,
GCAT
M.NspI, M.NspHI
RCATGY
2, 5
m5C
RCATGCATCNNNNN[NNNN]

LweI, PhaI, SfaNI
C

BmsI, BspST5I,
GCAT
M.Pam7686I
CCATGG
1, 6
m5C
CCATGGCATCNNNNN[NNNN],

LweI, PhaI, SfaNI
C

GCATCCATGG[NNNN]

BmsI, BspST5I,
GCAT
M.Pfa23572II
CGATCG
1, 6
m5C
CGATCGCATCNNNNN[NNNN],

LweI, PhaI, SfaNI
C

GCATCGATCG[NNNN]

BmsI, BspST5I,
GCAT
M.Pla5I
CTAG
1, 4
Unknown
CTAGCATCNNNNN[NNNN],

LweI, PhaI, SfaNI
C

GCATCTAGNN[NNNN]

BmsI, BspST5I,
GCAT
M.Sam53668II
CCATGG
1, 6
Unknown
CCATGGCATCNNNNN[NNNN],

LweI, PhaI, SfaNI
C

GCATCCATGG[NNNN]

BmsI, BspST5I,
GCAT
M.Sen1781III
CCWWGG
1, 6
Unknown
CCWWGGCATCNNNNN[NNNN],

LweI, PhaI, SfaNI
C

GCATCCWWGG[NNNN]

BmsI, BspST5I,
GCAT
M.SmoLVI, M.Spn23FII, M.Spn556I,
TCTAGA
1, 6
m6A
GCATCTAGAN[NNNN]

LweI, PhaI, SfaNI
C
M.Spn7465I, M.SpnRI, M.XbaI

BmsI, BspST5I,
GCAT
M.SonIII
GCANNNN
3, 9
m6A
GCATCNNGTC[NNNN]

LweI, PhaI, SfaNI
C

GTC

BmsI, BspST5I,
GCAT
M.TdeII
CTCTTC
1, 2
m4C,
GAAGAGCATCNNNNN[NNNN],

LweI, PhaI, SfaNI
C

m6A
GCATCTCTTC[NNNN]

BmsI, BspST5I,
GCAT
M.TspRI
CASTG
1, 5
m5C
CASTGCATCNNNNN[NNNN],

LweI, PhaI, SfaNI
C

GCATCASTGN[NNNN]

BmsI, BspST5I,
GCAT
M.Yen2516I
GCGCGC
2, 5
Unknown
GCGCGCATCNNNNN[NNNN]

LweI, PhaI, SfaNI
C

BmsI, BspST5I,
GCAT
M1.AgoG2I, M2.AgoG2I
CCAGT
1, 4
m4C
ACTGGCATCNNNNN[NNNN],

LweI, PhaI, SfaNI
C

GCATCCAGTN[NNNN]

BmsI, BspST5I,
GCAT
M1.BspACI
CCGC
1
m5C
GCGGCATCNNNNN[NNNN],

LweI, PhaI, SfaNI
C

GCATCCGCNN[NNNN]

BmsI, BspST5I,
GCAT
M1.BstF5I, M3.BstF5I, M2.Csp12AI
CATCC
3
m6A
GCATCCNNNN[NNNN]

LweI, PhaI, SfaNI
C

BmsI, BspST5I,
GCAT
M1.HgaI, M2.LlaJI
GACGC
4
m5C
GACGCATCNNNNN[NNNN]

LweI, PhaI, SfaNI
C

BmsI, BspST5I,
GCAT
M1.HphI
TCACC
2
m5C
GCATCACCNN[NNNN]

LweI, PhaI, SfaNI
C

BmsI, BspST5I,
GCAT
M1.Hpy32II
TCYYC
1
m6A
GCATCYYCNN[NNNN]

LweI, PhaI, SfaNI
C

BmsI, BspST5I,
GCAT
M1.HpyAII, M1.MboII, M1.NcuI
TCTTC
1
m6A
GCATCTTCNN[NNNN]

LweI, PhaI, SfaNI
C

BmsI, BspST5I,
GCAT
M1.LplAG30I
GAAGG
5
m5C
GCATCCTTCN[NNNN],

LweI, PhaI, SfaNI
C

GAAGGCATCNNNNN[NNNN]

BmsI, BspST5I,
GCAT
M1.Sgr13350III, M2.Sgr13350III
GAAGAGC
5, 6
m6A,
GAAGAGCATCNNNNN[NNNN]

LweI, PhaI, SfaNI
C

m4C

BmsI, BspST5I,
GCAT
M2.BceSIII, M1.Pru9I, M2.Pru91
ACGGC
2, 4
m4C
ACGGCATCNNNNN[NNNN]

LweI, PhaI, SfaNI
C

BmsI, BspST5I,
GCAT
M2.BstF5I
CATCC
2
m6A
GCATCCNNNN[NNNN]

LweI, PhaI, SfaNI
C

BmsI, BspST5I,
GCAT
M2.ClaSBI, M1.Fli6794IV, M2.HphI,
TCACC
1
m6A
GCATCACCNN[NNNN]

LweI, PhaI, SfaNI
C
M2.Ngo3502II, M1.Ngo6140II, M1.NgoAXVI,

M1.NgoBVIII, M1.NgoFA19I, M2.NgoMVIII,

M2.PspHUN102I, M1.Ssp10II

BmsI, BspST5I,
GCAT
M2.Hpy32II, M2.HpyAII, M2.MboII, M2.NcuI
TCTTC
2
m4C
GCATCTTCNN[NNNN]

LweI, PhaI, SfaNI
C

BmsI, BspST5I,
GCAT
M2.HpyAVI, M1.MnlI
GAGG
4
m5C
GCATCCTCNN[NNNN],

LweI, PhaI, SfaNI
C

GAGGCATCNNNNN[NNNN]

BmsI, BspST5I,
GCAT
M4.BstF5I, M1.Cin755I, M2.Cin755I,
CATCC
2, 3
m6A
GCATCCNNNN[NNNN]

LweI, PhaI, SfaNI
C
M1.CmeSR3I, M2.CmeSR3I, M1.Csp12AI,

M1.Csp323I, M2.Csp323I, M1.Csp410I,

M2.Csp410I, M.FokI, M1.Msp315I,

M2.Msp315I, M.Sau13435I, M.StsI,

M1.Tsu2489IV, M2.Tsu2489IV,

M3.Tsu2489IV

BpuSI, BslFI,
GGGA
M.AatII, M.Lsp6406III
GACGTC
2, 5
m6A
GGGACGTCNNNNNNN[NNNN]

BsmFI
C

BspLU11III,

BstOZ616I, FaqI

BpuSI, BslFI,
GGGA
M.AbaBs12I, M.Awo1030I, M.Cdu23823I,
CCWGG
1, 5
m5C
CCWGGGGACNNNNNNNNNN[NNNN],

BsmFI
C
M.Csa8155II, M.Cun9529I, M.EasL1Dcm,

CCWGGGACNNNNNNNNNN[NNNN],

BspLU11III,

M.Eba57Dcm, M.Ec134399Dcm,

GGGACCWGGNNNNNN[NNNN]

BstOZ616I, FaqI

M.Eco12761Dcm, M.Eco3609Dcm,

M.Eco4255Dcm, M.EcoVR50Dcm,

M.EsaSP1II, M.Kpn34618Dcm,

M.Kpn38547Dcm, M.Kpn39I, M.Kpn43816I,

M.Kpn677ORFDcm, M.Kpn928Dcm,

M.KpnNIH1Dcm, M.KpnNIH10Dcm,

M.KpnNIH30Dcm, M.Kva120Dcm, M.Lsp48I,

M.Pmi1I, M.SpaD2III, M.SplRp8II,

M.SptADcm, M.Tsu2489III

BpuSI, BslFI,
GGGA
M.AbrSp7I, M.Apa101655II, M.Ath21654I,
GANTC
2, 4
m6A
GGGACTCNNNNNNNN[NNNN]

BsmFI,
C
M.BdiNK6I, M.Bel76I, M.Bsp460I,

BspLU11III,

M.BspJKG1II, M.BspLA1II, M.BspRAC04II,

BstOZ616I, FaqI

M.CagII, M.CcrMI, M.CcrNAI, M.CnaPQ2I,

M.Csp16704III, M.CspNS6I, M.CviBI,

M.CviQVI, M.Eli8509I, M.Fnu419I, M.Fsp71I,

M.HbaI, M.HhaII, M.HinfI, M.HinHia1I,

M.Hpy298IV, M.Hpy299IV, M.Hpy32IV,

M.Hpy57IV, M.Hpy66IV, M.Hpy99IX,

M.HpyAIV, M.Hpy FIV, M.HpyUM032IV,

M.HpyUM037IV, M.Mbo45III, M.Msp10I,

M.Msp73BI, M.MspAL11I, M.MspLW5II,

M.NhaXII, M.NpeUS61I, M.OspHL35I,

M.Pin17395II, M.Pla5III, M.PspLM6I,

M.RbaNRL2I, M.Rgi145II, M.Rmo1926I,

M.RpaIII, M.RsaII, M.RshIII, M.Rsp8I,

M.SmeC3I, M.SmoLV, M.Sna24252I,

M.SscL1I, M.SspM41II, M.SstE37II, M.ThaIII,

M.Xsp91I, M.ZmoCP4I

BpuSI, BsIFI,
GGGA
M.AciI
CCGC
1, 3
m5C
GCGGGGACNNNNNNNNNN[NNNN],

BsmFI,
C

GCGGGACNNNNNNNNNN[NNNN],

BspLU11III,

GGGACCGCNNNNNNN[NNNN]

BstOZ616I, FaqI

BpuSI, BsIFI,
GGGA
M.AflIII
ACRYGT
2, 5
m4C
GGGACRYGTNNNNNN[NNNN]

BsmFI,
C

BspLU11III,

BstOZ616I, FaqI

BpuSI, BslFI,
GGGA
M.AhdI
GACNNNN
2, 10
m6A
GGGACNNNNNGTCNN[NNNN]

BsmFI,
C

NGTC

BspLU11III,

BstOZ616I, FaqI

BpuSI, Bs1FI,
GGGA
M.Ama55I, M.CnaPQ2III, M.Rmo1926II,
CGGCCG
1, 6
m5C
CGGCCGGGACNNNNNNNNNN[NNNN],

BsmFI,
C
M.SenC1765IV, M.SspG1I, M.XorPXII

GGGACGGCCGNNNNN[NNNN]

BspLU11III,

BstOZ616I, FaqI

BpuSI, BslFI,
GGGA
M.AquI
CYCGRG
1, 6
m5C
CYCGRGGGACNNNNNNNNNN[NNNN],

BsmFI,
C

CYCGGGGACNNNNNNNNNN[NNNN],

BspLU11III,

CYCGGGACNNNNNNNNNN[NNNN],

BstOZ616I, FaqI

GGGACYCGRGNNNNN[NNNN]

BpuSI, BslFI,
GGGA
M.AspU41II, M.BisIII, M.BsuW23II,
CCWGG
2, 4
m5C
CCWGGGACNNNNNNNNNN[NNNN]

BsmFI,
C
M.CnaPQ2II, M.EcoO6Dcm, M.Eco12581Dcm,

BspLU11III,

M.Eco1655Dcm, M.Eco29787Dcm,

BstOZ616I, FaqI

M.Eco3024Dcm, M.Eco3317Dcm,

M.Eco3325Dcm, M.Eco3740Dcm,

M.Eco3911Dcm, M.Eco4211Dcm,

M.Eco4465Dcm, M.Eco600Dcm,

M.Eco644Dcm, M.Eco86Dcm,

M.Eco9001Dcm, M.Eco9387Dcm,

M.EcoE1140Dcm, M.EcoE455Dcm,

M.EcoGDcm, M.EcoNU14Dcm, M.EcoRII,

M.Kpn223III, M.Kpn35657II, M.Kpn36Dcm,

M.Kpn555I, M.Kpn62629II, M.KpnAATV,

M.Kqu603Dcm, M.Psp345I, M.Sen158Dcm,

M.Sen641Dcm, M.SenA46Dcm,

M.SenAboDcm, M.TcoKWC4II

BpuSI, BslFI,
GGGA
M.AvaIX, M.Cfr10I, M.Nme18VI, M.VchAI,
RCCGGY
2, 5
m5C
GGGACCGGYNNNNNN[NNNN]

BsmFI,
C
M.Vch0395I

BspLU11III,

BstOZ616I, FaqI

BpuSI, BsIFI,
GGGA
M.BanLII, M.CpeAII, M.SinI
GGWCC
2, 4
m5C
GGGACCNNNNNNNNN[NNNN]

BsmFI,
C

BspLU11III,

BstOZ616I, FaqI

BpuSI, BsIFI,
GGGA
M.BceSII
GGWCC
1, 5
m5C
GGGACCNNNNNNNNN[NNNN]

BsmFI,
C

BspLU11III,

BstOZ616I, FaqI

BpuSI, BsIFI,
GGGA
M.BcoSlacII, M.MspLW5III, M.Nbr128I,
CTCGAG
1, 6
m4C
CTCGAGGGACNNNNNNNNNN[NNNN],

BsmFI,
C
M.Psy18884II, M.Tam77409II, M.TbrGE1II,

GGGACTCGAGNNNNN[NNNN]

BspLU11III,

M.TpaRLII

BstOZ616I, FaqI

BpuSI, BslFI,
GGGA
M.BepI, M.FpsJV, M.Gel16401III, M.Pdi8503I
CGCG
1, 4
m5C
CGCGGGACNNNNNNNNNN[NNNN],

BsmFI,
C

GGGACGCGNNNNNNN[NNNN]

BspLU11III,

BstOZ616I, FaqI

BpuSI, BsIFI,
GGGA
M.Bhy27164IV, M.BstNI, M.EcoDHB4Dcm,
CCWGG
2, 4
m4C
CCWGGGACNNNNNNNNNN[NNNN]

BsmFI,
C
M.EcoK54Dcm, M.Gth3570I, M.Hla49239I,

BspLU11III,

M.MvaI, M.Pdi8503II, M.Sso30807Dcm,

BstOZ616I, FaqI

M.YpeI, M.Ype41I, M.YpeShI

BpuSI, BsIFI,
GGGA
M.BsoBI
CYCGRG
1, 6
m4C
CYCGRGGGACNNNNNNNNNN[NNNN],

BsmFI,
C

CYCGGGGACNNNNNNNNNN[NNNN],

BspLU11III,

CYCGGGACNNNNNNNNNN[NNNN],

BstOZ616I, FaqI

GGGACYCGRGNNNNN[NNNN]

BpuSI, BslFI,
GGGA
M.BsuFI, M.CpeAIV, M.MspI
CCGG
1, 4
m5C
CCGGGGACNNNNNNNNNN[NNNN],

BsmFI,
C

CCGGGACNNNNNNNNNN[NNNN],

BspLU11III,

GGGACCGGNNNNNNN[NNNN]

BstOZ616I, FaqI

BpuSI, BslFI,
GGGA
M.Cab13497I, M.Dac11109III, M.Dco13527I,
CTAG
1, 4
m4C
CTAGGGACNNNNNNNNNN[NNNN],

BsmFI,
C
M.Djo10085I, M.Hbo11551I, M.Hka19301III,

GGGACTAGNNNNNNN[NNNN]

BspLU11III,

M.Hme33500I, M.HsaR1I, M.Hsi33800I,

BstOZ616I, FaqI

M.HspKM1WII, M.HspNII, M.HtuI, M.HvoDSI,

M.Lba2029II, M.Mbo45II, M.MjaI, M.MthZI,

M.Nas12278I, M.Rfl3010I, M.SsmMI

BpuSI, BsIFI,
GGGA
M.CagVIII
ACGCGT
2, 5
m4C
GGGACGCGTNNNNNN[NNNN]

BsmFI,
C

BspLU11III,

BstOZ616I, FaqI

BpuSI, BsIFI,
GGGA
M.Cdi630III
CCSSGG
2, 5
m4C
CCSSGGGACNNNNNNNNNN[NNNN],

BsmFI,
C

CCSGGGACNNNNNNNNNN[NNNN]

BspLU11III,

BstOZ616I, FaqI

BpuSI, BsIFI,
GGGA
M.Cfr9I, M.SmaI, M.Sma36365II, M.Xca85III,
CCCGGG
2, 5
m4C
CCCGGGGACNNNNNNNNNN[NNNN],

BsmFI,
C
M.XcyI, M.XmaI, M.XveII

CCCGGGACNNNNNNNNNN[NNNN]

BspLU11III,

BstOZ616I, FaqI

BpuSI, BslFI,
GGGA
M.CfrBI, M.Sen1735IV, M.SenAnaIV
CCWWGG
1, 6
m4C
CCWWGGGGACNNNNNNNNNN[NNNN],

BsmFI,
C

CCWWGGGACNNNNNNNNNN[NNNN],

BspLU11III,

GGGACCWWGGNNNNN[NNNN]

BstOZ616I, FaqI

BpuSI, BslFI,
GGGA
M.Cmu51329I, M.EcoJA03PDcm,
CCWGG
1, 5
Unknown
CCWGGGGACNNNNNNNNNN[NNNN],

BsmFI,
C
M.RorR1Dcm, M.Sen8391 Dcm, M.ZalSM2II

CCWGGGACNNNNNNNNNN[NNNN],

BspLU11III,

GGGACCWGGNNNNNN[NNNN]

BstOZ616I, FaqI

BpuSI, BslFI,
GGGA
M.CocIII, M.Fsi4947II, M.TdeIII,
GGNCC
1, 5
m4C
GGGACCNNNNNNNNN[NNNN]

BsmFI,
C
M.Tph12270I

BspLU11III,

BstOZ616I, FaqI

BpuSI, BsIFI,
GGGA
M.Cpa18696III, M.Ype19I, M.YpeDodI,
CCWGG
2, 4
Unknown
CCWGGGACNNNNNNNNNN[NNNN]

BsmFI,
C
M.YpeEDI, M.YpeJ9I, M.YpePGI

BspLU11III,

BstOZ616I, FaqI

BpuSI, BsIFI,
GGGA
M.DdeI
CTNAG
1, 5
m5C
CTNAGGGACNNNNNNNNNN[NNNN],

BsmFI,
C

GGGACTNAGNNNNNN[NNNN]

BspLU11III,

BstOZ616I, FaqI

BpuSI, BsIFI,
GGGA
M.DsaV, M.Ecl18kI, M.FpsJVI, M.NlaX,
CCNGG
2, 4
m5C
CCNGGGACNNNNNNNNNN[NNNN],

BsmFI,
C
M.SenPI, M.SsoII

CCGGGACNNNNNNNNNN[NNNN]

BspLU11III,

BstOZ616I, FaqI

BpuSI, BslFI,
GGGA
M.Eco29kI, M.NgoAIII, M.SacII
CCGCGG
2, 5
m5C
CCGCGGGACNNNNNNNNNN[NNNN]

BsmFI,
C

BspLU11III,

BstOZ616I, FaqI

BpuSI, BsIFI,
GGGA
M.EcoNI
CCTNNNN
2, 10
m4C
CCTNNNNNAGGGACNNNNNNNNNN[NNNN]

BsmFI,
C

NAGG

BspLU11III,

BstOZ616I, FaqI

BpuSI, BsIFI,
GGGA
M.EcoO109I
RGGNCCY
3, 5
m5C
GGGACCYNNNNNNNN[NNNN]

BsmFI,
C

BspLU11III,

BstOZ616I, FaqI

BpuSI, BslFI,
GGGA
M.EfaRFII, M.Mae7806II
CCGG
2, 3
m4C
CCGGGACNNNNNNNNNN[NNNN]

BsmFI,
C

BspLU11III,

BstOZ616I, FaqI

BpuSI, BslFI,
GGGA
M.FatI
CATG
1, 4
m5C
CATGGGACNNNNNNNNNN[NNNN],

BsmFI,
C

GGGACATGNNNNNNN[NNNN]

BspLU11III,

BstOZ616I, FaqI

BpuSI, BsIFI,
GGGA
M.FisAW1I, M.ThaI, M.Tph12270II
CGCG
1, 4
m4C
CGCGGGACNNNNNNNNNN[NNNN],

BsmFI,
C

GGGACGCGNNNNNNN[NNNN]

BspLU11III,

BstOZ616I, FaqI

BpuSI, BslFI,
GGGA
M.FnoB1II, M.Hal24586I, M.Hpy298V,
CCGG
1, 4
m4C
CCGGGGACNNNNNNNNNN[NNNN],

BsmFI,
C
M.Hpy299IX, M.Hpy30V, M.Hpy32XI,

CCGGGACNNNNNNNNNN[NNNN],

BspLU11III,

M.Hpy57V, M.Hpy66IX, M.Hpy99VIII,

GGGACCGGNNNNNNN[NNNN]

BstOZ616I, FaqI

M.HpyFV, M.HpyUM032IX, M.HpyUM037IX,

M.MjaVI, M.PspJDRI, M.TmeBII

BpuSI, BsIFI,
GGGA
M.FpsJI, M.HpaII, M.Nme18II, M.NmeAI
CCGG
2, 3
m5C
CCGGGACNNNNNNNNNN[NNNN]

BsmFI,
C

BspLU11III,

BstOZ616I, FaqI

BpuSI, BslFI,
GGGA
M.Gel16401IV
CTGG
3
m5C
CTGGGACNNNNNNNNNN[NNNN]

BsmFI,
C

BspLU11III,

BstOZ616I, FaqI

BpuSI, BsIFI,
GGGA
M.GsuRed1IV, M.Osp807II, M.Tth111I
GACNNNG
2, 8
m6A
GGGACNNNGTCNNNN[NNNN]

BsmFI,
C

TC

BspLU11III,

BstOZ616I, FaqI

BpuSI, BslFI,
GGGA
M.Hpy298II, M.Hpy299II, M.Hpy66II,
ACNGT
2, 4
m4C
GGGACNGTNNNNNNN[NNNN]

BsmFI,
C
M.HpyUM032II, M.HpyUM037II

BspLU11III,

BstOZ616I, FaqI

BpuSI, BslFI,
GGGA
M.Hpy30VIII, M.Hpy32VIII, M.Hpy57III,
TCNNGA
1, 6
m6A
TCGGGACNNNNNNNNNN[NNNN]

BsmFI,
C
M.Hpy66VI, M.Hpy99XVIII, M.HpyFVIII,

BspLU11III,

M.HpyPVIII

BstOZ616I, FaqI

BpuSI, BslFI,
GGGA
M.Hpy30X, M.Hpy57VIII, M.HpyFXI, M.MhuI
CTNAG
1, 5
m4C
CTNAGGGACNNNNNNNNNN[NNNN],

BsmFI,
C

GGGACTNAGNNNNNN[NNNN]

BspLU11III,

BstOZ616I, FaqI

BpuSI, BsIFI,
GGGA
M.Hpy99IV, M.HpyFORFX
CCNNGG
1, 6
m4C
CCNNGGGGACNNNNNNNNNN[NNNN],

BsmFI,
C

CCNNGGGACNNNNNNNNNN[NNNN],

BspLU11III,

CCNGGGACNNNNNNNNNN[NNNN],

BstOZ616I, FaqI

GGGACCNNGGNNNNN[NNNN]

BpuSI, BsIFI,
GGGA
M.Hpy99XI
ACGT
2, 3
m5C
GGGACGTNNNNNNNN[NNNN]

BsmFI,
C

BspLU11III,

BstOZ616I, FaqI

BpuSI, BsIFI,
GGGA
M.HpyAV, M.Nme18IV
GAAGG
3, 4
m6A,
GAAGGGACNNNNNNNNNN[NNNN]

BsmFI,
C

m5C

BspLU11III,

BstOZ616I, FaqI

BpuSI, BsIFI,
GGGA
M.Hpy D27I
GAGG
2, 4
m6A,
GGGACCTCNNNNNNN[NNNN],

BsmFI,
C

m5C
GAGGGGACNNNNNNNNNN[NNNN],

BspLU11III,

GAGGGACNNNNNNNNNN[NNNN]

BstOZ616I, FaqI

BpuSI, BsIFI,
GGGA
M.Mae7806III, M.PspPI, M.Sau96I
GGNCC
2, 4
m5C
GGGACCNNNNNNNNN[NNNN]

BsmFI
C

BspLU11III,

BstOZ616I, FaqI

BpuSI, BslFI,
GGGA
M.MjaII
GGNCC
1, 5
m5C
GGGACCNNNNNNNNN[NNNN]

BsmFI,
C

BspLU11III,

BstOZ616I, FaqI

BpuSI, BsIFI,
GGGA
M.MlyI, M.Pen113I
GASTC
2, 4
m6A
GGGACTCNNNNNNNN[NNNN]

BsmFI,
C

BspLU11III,

BstOZ616I, FaqI

BpuSI, BslFI,
GGGA
M.Msp1II
CCAGG
5
m4C
GGGACCTGGNNNNNN[NNNN],

BsmFI,
C

CCAGGGGACNNNNNNNNNN[NNNN],

BspLU11III,

CCAGGGACNNNNNNNNNN[NNNN]

BstOZ616I, FaqI

BpuSI, BslFI,
GGGA
M.Msp42II
CCWGG
1, 5
m4C
CCWGGGGACNNNNNNNNNN[NNNN],

BsmFI,
C

CCWGGGACNNNNNNNNNN[NNNN],

BspLU11III,

GGGACCWGGNNNNNN[NNNN]

BstOZ616I, FaqI

BpuSI, BsIFI,
GGGA
M.NgoAXV, M.NgoMV
GGNNCC
2, 5
m5C
GGGACCNNNNNNNNN[NNNN]

BsmFI,
C

BspLU11III,

BstOZ616I, FaqI

BpuSI, BsIFI,
GGGA
M.Nme18III
TCTGG
2, 4
m5C
TCTGGGACNNNNNNNNNN[NNNN]

BsmFI,
C

BspLU11III,

BstOZ616I, FaqI

BpuSI, BslFI,
GGGA
M.NmeDI
VCCGGY
2, 5
m5C
RCCGGGGACNNNNNNNNNN[NNNN],

BsmFI,
C

GGGACCGGBNNNNNN[NNNN],

BspLU11III,

RCCGGGACNNNNNNNNNN[NNNN]

BstOZ616I, FaqI

BpuSI, BslFI,
GGGA
M.NmeMC58III
CCAGA
1
m5C
TCTGGGGACNNNNNNNNNN[NNNN],

BsmFI,
C

TCTGGGACNNNNNNNNNN[NNNN],

BspLU11III,

GGGACCAGANNNNNN[NNNN]

BstOZ616I, FaqI

BpuSI, BsIFI,
GGGA
M.Nsp209III, M.Nsp51109II
CTCGAG
1, 6
m5C
CTCGAGGGACNNNNNNNNNN[NNNN],

BsmFI,
C

GGGACTCGAGNNNNN[NNNN]

BspLU11III,

BstOZ616I, FaqI

BpuSI, BslFI,
GGGA
M.NspI, M.NspHI
RCATGY
2, 5
m5C
GGGACATGYNNNNNN[NNNN]

BsmFI,
C

BspLU11III,

BstOZ616I, FaqI

BpuSI, Bs1FI,
GGGA
M.Pam7686I
CCATGG
1, 6
m5C
CCATGGGGACNNNNNNNNNN[NNNN],

BsmFI,
C

CCATGGGACNNNNNNNNNN[NNNN],

BspLU11III,

GGGACCATGGNNNNN[NNNN]

BstOZ616I, FaqI

BpuSI, BslFI,
GGGA
M.Pfa23572II
CGATCG
1, 6
m5C
CGATCGGGACNNNNNNNNNN[NNNN],

BsmFI,
C

GGGACGATCGNNNNN[NNNN]

BspLU11III,

BstOZ616I, FaqI

BpuSI, BslFI,
GGGA
M.Pla5I
CTAG
1, 4
Unknown
CTAGGGACNNNNNNNNNN[NNNN],

BsmFI,
C

GGGACTAGNNNNNNN[NNNN]

BspLU11III,

BstOZ616I, FaqI

BpuSI, BslFI,
GGGA
M.Sam53668II
CCATGG
1, 6
Unknown
CCATGGGGACNNNNNNNNNN[NNNN],

BsmFI,
C

CCATGGGACNNNNNNNNNN[NNNN],

BspLU11III,

GGGACCATGGNNNNN[NNNN]

BstOZ616I, FaqI

BpuSI, BsIFI,
GGGA
M.Sen1781III
CCWWGG
1, 6
Unknown
CCWWGGGGACNNNNNNNNNN[NNNN],

BsmFI,
C

CCWWGGGACNNNNNNNNNN[NNNN],

BspLU11III,

GGGACCWWGGNNNNN[NNNN]

BstOZ616I, FaqI

BpuSI, BsIFI,
GGGA
M.SonIII
GACNNNN
2, 8
m6A
GGGACNNNNTGCNNN[NNNN]

BsmFI,
C

TGC

BspLU11III,

BstOZ616I, FaqI

BpuSI, BsIFI,
GGGA
M.TdeII
CTCTTC
1, 2
m4C
GAAGAGGGACNNNNNNNNNN[NNNN],

BsmFI,
C

m6A
GGGACTCTTCNNNNN[NNNN]

BspLU11III,

BstOZ616I, FaqI

BpuSI, BsIFI,
GGGA
M.TspRI
CASTG
1, 5
m5C
CASTGGGACNNNNNNNNNN[NNNN],

BsmFI,
C

GGGACASTGNNNNNN[NNNN]

BspLU11III,

BstOZ616I, FaqI

BpuSI, BslFI,
GGGA
M1.AgoG2I, M2.AgoG2I
CCAGT
1, 4
m4C
ACTGGGGACNNNNNNNNNN[NNNN],

BsmFI,
C

ACTGGGACNNNNNNNNNN[NNNN],

BspLU11III,

GGGACTGGNNNNNNN[NNNN],

BstOZ616I, FaqI

GGGACCAGTNNNNNN[NNNN]

BpuSI, BsIFI,
GGGA
M1.Bag18758II, M2.Bag18758II
CACGGG
3, 5
Unknown
CACGGGGACNNNNNNNNNN[NNNN],

BsmFI,
C

CACGGGACNNNNNNNNNN[NNNN]

BspLU11III,

BstOZ616I, FaqI

BpuSI, BsIFI,
GGGA
M1.BceSIII
ACGGC
2
m4C
GGGACGGCNNNNNNN[NNNN]

BsmFI,
C

BspLU11III,

BstOZ616I, FaqI

BpuSI, BsIFI,
GGGA
M1.BcnI, M2.BcnI, M.NciI
CCSGG
2, 4
m4C
CCSGGGACNNNNNNNNNN[NNNN],

BsmFI,
C

CCGGGACNNNNNNNNNN[NNNN]

BspLU11III,

BstOZ616I, FaqI

BpuSI, BsIFI,
GGGA
M1.BfiI
ACTGGG
5
m4C
ACTGGGGACNNNNNNNNNN[NNNN],

BsmFI
C

ACTGGGACNNNNNNNNNN[NNNN]

BspLU11III,

BstOZ616I, FaqI

BpuSI, BsIFI,
GGGA
M1.BspACI
CCGC
1
m5C
GCGGGGACNNNNNNNNNN[NNNN],

BsmFI,
C

GCGGGACNNNNNNNNNN[NNNN],

BspLU11III,

GGGACCGCNNNNNNN[NNNN]

BstOZ616I, FaqI

BpuSI,
GGGA
M1.Dsh12II, M2.Dsh12II
GACGGC
3, 4
m4C
GGGACGGCNNNNNNN[NNNN]

BsIFI,
C

BsmFI,

BspLU11III,

BstOZ616I, FaqI

BpuSI, BsIFI,
GGGA
M1.Hpy32II
GRRGA
5
m6A
GGGGACNNNNNNNNNN[NNNN]

BsmFI,
C

BspLU11III,

BstOZ616I, FaqI

BpuSI, BslFI,
GGGA
M1.LplAG30I
GAAGG
5
m5C
GGGACCTTCNNNNNN[NNNN],

BsmFI,
C

GAAGGGGACNNNNNNNNNN[NNNN],

BspLU11III,

GAAGGGACNNNNNNNNNN[NNNN]

BstOZ616I, FaqI

BpuSI, BslFI,
GGGA
M2.BceSIII, M1.Pru9I, M2.Pru9I
ACGGC
2, 4
m4C
GGGACGGCNNNNNNN[NNNN]

BsmFI,
C

BspLU11III,

BstOZ616I, FaqI

BpuSI, BsIFI,
GGGA
M2.BfiI
ACTGGG
2
m4C
GGGACTGGGNNNNNN[NNNN]

BsmFI,
C

BspLU11III,

BstOZ616I, FaqI

BpuSI, BslFI,
GGGA
M2.BfuAI
ACCTGC
2
m5C
GGGACCTGCNNNNNN[NNNN]

BsmFI,
C

BspLU11III,

BstOZ616I, FaqI

BpuSI, BslFI,
GGGA
M2.HgaI, M1.LlaJI
GACGC
3
m5C
GGGACGCNNNNNNNN[NNNN]

BsmFI,
C

BspLU11III,

BstOZ616I, FaqI

BpuSI, BslFI,
GGGA
M2.HpyAVI, M1.MnlI
GAGG
4
m5C
GGGACCTCNNNNNNN[NNNN],

BsmFI,
C

GAGGGGACNNNNNNNNNN[NNNN],

BspLU11III,

GAGGGACNNNNNNNNNN[NNNN]

BstOZ616I, FaqI

BpuSI, BslFI,
GGGA
M2.Nme579IV, M2.NmeMC58II
GACGC
2
m6A
GGGACGCNNNNNNNN[NNNN]

BsmFI,
C

BspLU11III,

BstOZ616I, FaqI

CseI, HgaI
GACG
M.AbaBs12I, M.Awo1030I, M.Cdu23823I,
CCWGG
1, 5
m5C
CCWGGACGCNNNNN[NNNNN],

C
M.Csa8155II, M.Cun9529I, M.EasL1Dcm,

GACGCCWGGN[NNNNN]

M.Eba57Dcm, M.Ec134399Dcm,

M.Eco12761Dcm, M.Eco3609Dcm,

M.Eco4255Dcm, M.EcoVR50Dcm,

M.EsaSP1II, M.Kpn34618Dcm,

M.Kpn38547Dcm, M.Kpn39I, M.Kpn43816I,

M.Kpn677ORFDcm, M.Kpn928Dcm,

M.KpnNIH1Dcm, M.KpnNIH10Dcm,

M.KpnNIH30Dcm, M.Kva120Dcm, M.Lsp48I,

M.Pmi1I, M.SpaD2III, M.SplRp8II,

M.SptADcm, M.Tsu2489III

CseI, HgaI
GACG
M.AccI, M.FpsFPG3II, M.SdeAIV
GTMKAC
2, 5
m6A
GTMGACGCNNNNN[NNNNN]

C

CseI, HgaI
GACG
M.AciI
CCGC
1, 3
m5C
GCGGACGCNNNNN[NNNNN],

C

GACGCGGNNN[NNNNN],

GACGCCGCNN[NNNNN]

CseI, HgaI
GACG
M.AflIII
ACRYGT
2, 5
m4C
GACGCGTNNN[NNNNN]

C

CseI, HgaI
GACG
M.Alw26I
GAGAC
4, 3
m6A,
GAGACGCNNNNN[NNNNN]

C

m5C

CseI, HgaI
GACG
M.Ama55I, M.CnaPQ2III, M.Rmo1926II,
CGGCCG
1, 6
m5C
CGGCCGACGCNNNNN[NNNNN],

C
M.SenC1765IV, M.SspG1I, M.XorPXII

GACGCGGCCG[NNNNN]

CseI, HgaI
GACG
M.AorT14III, M.GsuRed1III, M.SalI
GTCGAC
2, 5
m6A
GTCGACGCNNNNN[NNNNN]

C

CseI, HgaI
GACG
M.AquI
CYCGRG
1, 6
m5C
CYCGRGACGCNNNNN[NNNNN],

C

GACGCYCGRG[NNNNN]

CseI, HgaI
GACG
M.AvaIX, M.Cfr10I, M.Nme18VI, M.VchAI,
RCCGGY
2, 5
m5C
GACGCCGGYN[NNNNN]

C
M.Vch0395I

CseI, HgaI
GACG
M.BamWS8I, M.CthV, M.EfaRFI, M.Saf8902II,
GCWGC
2, 4
m5C
GACGCWGCNN[NNNNN]

C
M.TacII, M.TcoKWC4III

CseI, HgaI
GACG
M.BbvI, M.BceSIV
GCAGC
2, 4
m5C
GACGCTGCNN[NNNNN],

C

GACGCAGCNN[NNNNN]

CseI, HgaI
GACG
M.BcoSlacII, M.MspLW5III, M.Nbr128I,
CTCGAG
1, 6
m4C
CTCGAGACGCNNNNN[NNNNN],

C
M.Psy18884II, M.Tam77409II, M.TbrGE1II,

GACGCTCGAG[NNNNN]

M.TpaRLII

CseI, HgaI
GACG
M.BepI, M.FpsJV, M.Gel16401III, M.Pdi8503I
CGCG
1, 4
m5C
GACGCGNNNN[NNNNN],

C

CGCGACGCNNNNN[NNNNN]

CseI, HgaI
GACG
M.BsoBI
CYCGRG
1, 6
m4C
CYCGRGACGCNNNNN[NNNNN],

C

GACGCYCGRG[NNNNN]

CseI, HgaI
GACG
M.BsuFI, M.CpeAIV, M.MspI
CCGG
1, 4
m5C
CCGGACGCNNNNN[NNNNN],

C

GACGCCGGNN[NNNNN]

CseI, HgaI
GACG
M.Cab13497I, M.Dac11109III, M.Dco13527I,
CTAG
1, 4
m4C
CTAGACGCNNNNN[NNNNN],

C
M.Djo10085I, M.Hbo11551I, M.Hka19301III,

GACGCTAGNN[NNNNN]

M.Hme33500I, M.HsaR1I, M.Hsi33800I,

M.HspKM1WII, M.HspNII, M.HtuI, M.HvoDSI,

M.Lba2029II, M.Mbo45II, M.MjaI, M.MthZI,

M.Nas12278I, M.Rfl3010I, M.SsmMI

CseI, HgaI
GACG
M.Cac8I
GCNNGC
2, 5
m5C
GACGCNNGCN[NNNNN]

C

CseI, HgaI
GACG
M.CagVI
TCCGGA
1, 6
m6A
TCCGGACGCNNNNN[NNNNN]

C

CseI, HgaI
GACG
M.CagVIII
ACGCGT
2, 5
m4C
GACGCGTNNN[NNNNN]

C

CseI, HgaI
GACG
M.CcrNAV
YGCCGGCR
3, 6
m5C
GACGCCGGCR[NNNNN]

C

CseI, HgaI
GACG
M.Cdi630IV, M.Ckr177III, M.CmaLM2II,
GCNGC
2, 4
m5C
GACGCNGCNN[NNNNN]

C
M.CocII, M.Fnu4HI, M.Fsp 4HI

CseI, HgaI
GACG
M.CfrBI, M.Sen1735IV, M.SenAnaIV
CCWWGG
1, 6
m4C
CCWWGGACGCNNNNN[NNNNN],

C

GACGCCWWGG[NNNNN]

CseI, HgaI
GACG
M.Cmu51329I, M.EcoJA03PDcm,
CCWGG
1, 5
Unknown
CCWGGACGCNNNNN[NNNNN],

C
M.RorR1Dcm, M.Sen8391Dcm, M.ZalSM2II

GACGCCWGGN[NNNNN]

CseI, HgaI
GACG
M.CocI, M.Fli6794II, M.HincII, M.HindII,
GTYRAC
2, 5
m6A
GTYGACGCNNNNN[NNNNN]

C
M.HinHia1II

CseI, HgaI
GACG
M.CsiFSMII, M.MfoCT6IV, M.NgoAIV,
GCCGGC
2, 5
m5C
GACGCCGGCN[NNNNN]

C
M.NgoMIV, M1.Sgr13350II

CseI, HgaI
GACG
M.CviJI
VGCY
2, 3
m5C
GACGCYNNNN[NNNNN]

C

CseI, HgaI
GACG
M.CviQIII, M.CviSIII, M.Hpy299XI,
TCGA
1, 4
m6A
TCGACGCNNNNN[NNNNN]

C
M.Hpy30II, M.Hpy32X, M.Hpy99VII,

M.HpyAX, M.HpyFII, M.HpyUM032XVII,

M.Tam77409III, M.TaqI, M.TpaRLI,

M.TthHB8I

CseI, HgaI
GACG
M.DdeI
CTNAG
1, 5
m5C
CTNAGACGCNNNNN[NNNNN],

C

GACGCTNAGN[NNNNN]

CseI, HgaI
GACG
M.Esp3I
GAGACG
4, 3
m6A,
GAGACGCNNNNN[NNNNN]

C

m5C

CseI, HgaI
GACG
M.FatI
CATG
1, 4
m5C
CATGACGCNNNNN[NNNNN],

C

GACGCATGNN[NNNNN]

CseI, HgaI
GACG
M.FisAW1I, M.ThaI, M.Tph12270II
CGCG
1, 4
m4C
GACGCGNNNN[NNNNN],

C

CGCGACGCNNNNN[NNNNN]

CseI, HgaI
GACG
M.FnoB1II, M.Hal24586I, M.Hpy298V,
CCGG
1, 4
m4C
CCGGACGCNNNNN[NNNNN],

C
M.Hpy299IX, M.Hpy30V, M.Hpy32XI,

GACGCCGGNN[NNNNN]

M.Hpy57V, M.Hpy66IX, M.Hpy99VIII,

M.HpyFV, M.HpyUM032IX, M.HpyUM037IX,

M.MjaVI, M.PspJDRI, M.TmeBII

CseI, HgaI
GACG
M.GsuRed1IV, M.Osp807II, M.Tth111I
GACNNNG
2, 8
m6A
GACGCNGTCN[NNNNN]

C

TC

CseI, HgaI
GACG
M.HhaI, M.HinP1I, M.Hpy99III, M.HpyAVIII,
GCGC
2, 3
m5C
GACGCGCNNN[NNNNN]

C
M.HpyUM032XV

CseI, HgaI
GACG
M.Hka19301IV
TCGCGA
2, 5
m4C
TCGCGACGCNNNNN[NNNNN]

C

CseI, HgaI
GACG
M.Hme33500II
HGCWGCK
3
m4C
GACGCWGCKN[NNNNN]

C

CseI, HgaI
GACG
M.Hpy188I, M.Hpy298IX, M.Hpy299V,
TCNGA
1, 5
m6A
TCNGACGCNNNNN[NNNNN]

C
M.Hpy66V, M.HpyUM032V

CseI, HgaI
GACG
M.Hpy30IX, M.Hpy32IX, M.Hpy57IX,
GTNNAC
2, 5
m6A
GTNGACGCNNNNN[NNNNN]

C
M.Hpy66XIII, M.Hpy8I, M.HpyFIX,

M.HpyUM032XI, M.HpyUM037V, M.MjaIV,

M.MspRAC08II

CseI, HgaI
GACG
M.Hpy30VIII, M.Hpy32VIII, M.Hpy57III,
TCNNGA
1, 6
m6A
TCNNGACGCNNNNN[NNNNN]

C
M.Hpy66VI, M.Hpy99XVIII, M.HpyFVIII,

M.HpyPVIII

CseI, HgaI
GACG
M.Hpy30X, M.Hpy57VIII, M.HpyFXI, M.MhuI
CTNAG
1, 5
m4C
CTNAGACGCNNNNN[NNNNN],

C

GACGCTNAGN[NNNNN]

CseI, HgaI
GACG
M.Hpy66I, M.Hpy99I
CGWCG
2, 4
m4C
CGACGCNNNNN[NNNNN]

C

CseI, HgaI
GACG
M.Hpy99II, M.Tsp45I, M.Tsu2489II
GTSAC
2, 4
m6A
GTGACGCNNNNN[NNNNN]

C

CseI, HgaI
GACG
M.Hpy99IV, M.HpyFORFX
CCNNGG
1, 6
m4C
CCNNGGACGCNNNNN[NNNNN],

C

GACGCCNNGG[NNNNN]

CseI, HgaI
GACG
M.HpyD27I
GAGG
2, 4
m6A,
GACGCCTCNN[NNNNN],

C

m5C
GAGGACGCNNNNN[NNNNN]

CseI, HgaI
GACG
M.Lph145I, M.Lph93I
GGWGA
5
m6A
GGWGACGCNNNNN[NNNNN]

C

CseI, HgaI
GACG
M.Msp1II
CCAGG
5
m4C
GACGCCTGGN[NNNNN],

C

CCAGGACGCNNNNN[NNNNN]

CseI, HgaI
GACG
M.Msp42II
CCWGG
1, 5
m4C
CCWGGACGCNNNNN[NNNNN],

C

GACGCCWGGN[NNNNN]

CseI, HgaI
GACG
M.NheI
GCTAGC
1, 6
m4C
GACGCTAGCN[NNNNN]

C

CseI, HgaI
GACG
M.Nme18III
CCAGA
2, 4
m5C
CCAGACGCNNNNN[NNNNN]

C

CseI, HgaI
GACG
M.Nme18V
GCGCGC
2, 5
m5C
GACGCGCGCN[NNNNN]

C

CseI, HgaI
GACG
M.NmeDI
VCCGGY
2, 5
m5C
GACGCCGGBN[NNNNN]

C

CseI, HgaI
GACG
M.NmeMC58III
CCAGA
1
m5C
TCTGGACGCNNNNN[NNNNN],

C

GACGCCAGAN[NNNNN]

CseI, HgaI
GACG
M.Nsp209III, M.Nsp51109II
CTCGAG
1, 6
m5C
CTCGAGACGCNNNNN[NNNNN],

C

GACGCTCGAG[NNNNN]

CseI, HgaI
GACG
M.NspI, M.NspHI
RCATGY
2, 5
m5C
GACGCATGYN[NNNNN]

C

CseI, HgaI
GACG
M.Pam7686I
CCATGG
1, 6
m5C
CCATGGACGCNNNNN[NNNNN],

C

GACGCCATGG[NNNNN]

CseI, HgaI
GACG
M.Pfa23572II
CGATCG
1, 6
m5C
CGATCGACGCNNNNN[NNNNN],

C

GACGCGATCG[NNNNN]

CseI, HgaI
GACG
M.Pla5I
CTAG
1, 4
Unknown
CTAGACGCNNNNN[NNNNN],

C

GACGCTAGNN[NNNNN]

CseI, HgaI
GACG
M.Sam53668II
CCATGG
1, 6
Unknown
CCATGGACGCNNNNN[NNNNN],

C

GACGCCATGG[NNNNN]

CseI, HgaI
GACG
M.Sen1781III
CCWWGG
1, 6
Unknown
CCWWGGACGCNNNNN[NNNNN],

C

GACGCCWWGG[NNNNN]

CseI, HgaI
GACG
M.SmoLVI, M.Spn23FII, M.Spn556I,
TCTAGA
1, 6
m6A
TCTAGACGCNNNNN[NNNNN]

C
M.Spn7465I, M.SpnRI, M.XbaI

CseI, HgaI
GACG
M.SonIII
GACNNNN
2, 8
m6A
GACGCNNTGC[NNNNN]

C

TGC

CseI, HgaI
GACG
M.TdeII
CTCTTC
1, 2
m4C,
GAAGAGACGCNNNNN[NNNNN],

C

m6A
GACGCTCTTC[NNNNN]

CseI, HgaI
GACG
M.TspRI
CASTG
1, 5
m5C
CASTGACGCNNNNN[NNNNN],

C

GACGCASTGN[NNNNN]

CseI, HgaI
GACG
M.Yen2516I
GCGCGC
2, 5
Unknown
GACGCGCGCN[NNNNN]

C

CseI, HgaI
GACG
M1.AgoG2I, M2.AgoG2I
CCAGT
1, 4
m4C
ACTGGACGCNNNNN[NNNNN],

C

GACGCCAGTN[NNNNN]

CseI, HgaI
GACG
M1.BspACI
CCGC
1
m5C
GCGGACGCNNNNN[NNNNN],

C

GACGCCGCNN[NNNNN]

CseI, HgaI
GACG
M1.HphI
GGTGA
4
m5C
GGTGACGCNNNNN[NNNNN]

C

CseI, HgaI
GACG
M1.Hpy32II
GRRGA
5
m6A
GRRGACGCNNNNN[NNNNN]

C

CseI, HgaI
GACG
M1.HpyAII, M1.MboII, M1.NcuI
GAAGA
5
m6A
GAAGACGCNNNNN[NNNNN]

C

CseI, HgaI
GACG
M1.LplAG30I
GAAGG
5
m5C
GACGCCTTCN[NNNNN],

C

GAAGGACGCNNNNN[NNNNN]

CseI, HgaI
GACG
M1.Sgr13350III, M2.Sgr13350III
GCTCTTC
2, 3
m4C,
GACGCTCTTC[NNNNN]

C

m6A

CseI, HgaI
GACG
M2.BceSIII, M1.Pru9I, M2.Pru9I
GCCGT
2, 4
m4C
GACGCCGTNN[NNNNN]

C

CseI, HgaI
GACG
M2.ClaSBI, M1.Fli6794IV, M2.HphI,
GGTGA
5
m6A
GGTGACGCNNNNN[NNNNN]

C
M2.Ngo3502II, M1.Ngo6140II, M1.NgoAXVI,

M1.NgoBVIII, M1.NgoFA19I, M2.NgoMVIII,

M2.PspHUN102I, M1.Ssp10II

CseI, HgaI
GACG
M2.Hpy32II, M2.HpyAII, M2.MboII, M2.NcuI
GAAGA
4
m4C
GAAGACGCNNNNN[NNNNN]

C

CseI, HgaI
GACG
M2.HpyAVI, M1.MnlI
GAGG
4
m5C
GACGCCTCNN[NNNNN],

C

GAGGACGCNNNNN[NNNNN]

CseI, HgaI
GACG
M2.RspA76I
GGGAC
4
m6A
GGGACGCNNNNN[NNNNN]

C

FokI
GGAT
M.AbaBs12I, M.Awo1030I, M.Cdu23823I,
CCWGG
1, 5
m5C
CCWGGGATGNNNNNNNNN[NNNN],

G
M.Csa8155II, M.Cun9529I, M.EasL1Dcm,

CCWGGATGNNNNNNNNN[NNNN]

M.Eba57Dcm, M.Ec134399Dcm,

M.Eco12761Dcm, M.Eco3609Dcm,

M.Eco4255Dcm, M.EcoVR50Dcm,

M.EsaSP1II, M.Kpn34618Dcm,

M.Kpn38547Dcm, M.Kpn39I, M.Kpn43816I,

M.Kpn677ORFDcm, M.Kpn928Dcm,

M.KpnNIH1Dcm, M.KpnNIH10Dcm,

M.KpnNIH30Dcm, M.Kva120Dcm, M.Lsp48I,

M.Pmi1I, M.SpaD2III, M.SplRp8II,

M.SptADcm, M.Tsu2489III

FokI
GGAT
M.AciI
GCGG
2, 4
m5C
GCGGGATGNNNNNNNNN[NNNN],

G

GCGGATGNNNNNNNNN[NNNN]

FokI
GGAT
M.Ama55I, M.CnaPQ2III, M.Rmo1926II,
CGGCCG
1, 6
m5C
CGGCCGGATGNNNNNNNNN[NNNN]

G
M.SenC1765IV, M.SspG1I, M.XorPXII

FokI
GGAT
M.AquI
CYCGRG
1, 6
m5C
CYCGRGGATGNNNNNNNNN[NNNN],

G

CYCGGGATGNNNNNNNNN[NNNN]

FokI
GGAT
M.AspU41II, M.BisIII, M.BsuW23II,
CCWGG
2, 4
m5C
CCWGGATGNNNNNNNNN[NNNN]

G
M.CnaPQ2II, M.EcoO6Dcm, M.Eco12581Dcm,

M.Eco1655Dcm, M.Eco29787Dcm,

M.Eco3024Dcm, M.Eco3317Dcm,

M.Eco3325Dcm, M.Eco3740Dcm,

M.Eco3911Dcm, M.Eco4211Dcm,

M.Eco4465Dcm, M.Eco600Dcm,

M.Eco644Dcm, M.Eco86Dcm,

M.Eco9001Dcm, M.Eco9387Dcm,

M.EcoE1140Dcm, M.EcoE455Dcm,

M.EcoGDcm, M.EcoNU14Dcm, M.EcoRII,

M.Kpn223III, M.Kpn35657II, M.Kpn36Dcm,

M.Kpn555I, M.Kpn62629II, M.KpnAATV,

M.Kqu603Dcm, M.Psp345I, M.Sen158Dcm,

M.Sen641Dcm, M.SenA46Dcm,

M.SenAboDcm, M.TcoKWC4II

FokI
GGAT
M.AvaV, M.SliSI
GATC
1, 4
m4C
GGATGATCNNNNNN[NNNN]

G

FokI
GGAT
M.BceSII
GGWCC
1, 5
m5C
GGATGGWCCNNNNN[NNNN]

G

FokI
GGAT
M.BcoSlacII, M.MspLW5III, M.Nbr128I,
CTCGAG
1, 6
m4C
CTCGAGGATGNNNNNNNNN[NNNN]

G
M.Psy18884II, M.Tam77409II, M.TbrGE1II,

M.TpaRLII

FokI
GGAT
M.BepI, M.FpsJV, M.Gel16401III, M.Pdi8503I
CGCG
1, 4
m5C
CGCGGATGNNNNNNNNN[NNNN]

G

FokI
GGAT
M.BfaSIII, M.Ccl10114I, M.CfrB38I,
ATGCAT
2, 5
m6A
GGATGCATNNNNNN[NNNN]

G
M.CfrH1I, M.EcoGVI, M.EcoKII,

M.Fnu10562II, M.SbaUIV, M.Sbo12419III,

M.Sbo268II, M.Sen10384II, M.Sen10708II,

M.Sen1080II, M.Sen1175III, M.Sen13IV,

M.Sen13311IV, M.Sen1387II, M.Sen1427III,

M.Sen14882III, M.Sen15791III, M.Sen158III,

M.Sen16I, M.Sen1655III, M.Sen1676III,

M.Sen1677III, M.Sen1728III, M.Sen1735III,

M.Sen1736IV, M.Sen1764III, M.Sen1766III,

M.Sen1781IV, M.Sen1783III, M.Sen18569II,

M.Sen1878III, M.Sen1880III, M.Sen1896III,

M.Sen1898III, M.Sen1899III, M.Sen1903III,

M.Sen1906III, M.Sen1908III, M.Sen1910III,

M.Sen1921III, M.Sen1927II, M.Sen195II,

M.Sen2050II, M.Sen2064III, M.Sen2069III,

M.Sen22462I, M.Sen255II, M.Sen318III,

M.Sen3387III, M.Sen3890III, M.Sen483III,

M.Sen624III, M.Sen641III, M.Sen7378II,

M.Sen8391III, M.Sen8692III, M.SenA46III,

M.SenAbaII, M.SenAboII, M.SenAnaIII,

M.SenC1736III, M.SenC1765III,

M.SenC1808IV, M.SenC1810III, M.SenJIII,

M.SenL1II, M.SenL15III, M.SenN1660II,

M.SenSARA26II, M.SenSPBIII, M.SenTFIII

FokI
GGAT
M.Bhy27164IV, M.BstNI, M.EcoDHB4Dcm,
CCWGG
2, 4
m4C
CCWGGATGNNNNNNNNN[NNNN]

G
M.EcoK54Dcm, M.Gth3570I, M.Hla49239I,

M.MvaI, M.Pdi8503II, M.Sso30807Dcm,

M.YpeI, M.Ype41I, M.YpeShI

FokI
GGAT
M.BsoBI
CYCGRG
1, 6
m4C
CYCGRGGATGNNNNNNNNN[NNNN],

G

CYCGGGATGNNNNNNNNN[NNNN]

FokI
GGAT
M.Bsp14III, M.Bsp3003I, M.Bsp3004I,
GATC
1, 4
m5C
GGATGATCNNNNNN[NNNN]

G
M.CalB3III, M.CpeAIII, M.Esu35585I,

M.FpsJIV, M.Lba2031I, M.Lbo2004I,

M.LlaKR2I, M.Lmo1042I, M.Lmo7956II,

M.Lsp10393II, M.MspI7I, M.Sau3AI, M.Sba3I,

M.Sin395I, M.Spn23FI, M.Sth368I

FokI
GGAT
M.BstXI
CCANNNN
3, 10
m6A
CCANNNGGATGGNNNNNNNN[NNNN]

G

NNTGG

FokI
GGAT
M.BsuFI, M.CpeAIV, M.MspI
CCGG
1, 4
m5C
CCGGGATGNNNNNNNNN[NNNN],

G

CCGGATGNNNNNNNNN[NNNN]

FokI
GGAT
M.Cab13497I, M.Dac11109III, M.Dco13527I,
CTAG
1, 4
m4C
CTAGGATGNNNNNNNNN[NNNN]

G
M.Djo10085I, M.Hbo11551I, M.Hka19301III,

M.Hme33500I, M.HsaR1I, M.Hsi33800I,

M.Hsp KM1WII, M.HspNII, M.HtuI, M.HvoDSI,

M.Lba2029II, M.Mbo45II, M.MjaI, M.MthZI,

M.Nas12278I, M.Rfl3010I, M.SsmMI

FokI
GGAT
M.CagVI
TCCGGA
1, 6
m6A
TCCGGATGNNNNNNNNN[NNNN]

G

FokI
GGAT
M.Cdi630III
CCSSGG
2, 5
m4C
CCSSGGATGNNNNNNNNN[NNNN]

G

FokI
GGAT
M.Cfr9I, M.SmaI, M.Sma36365II, M.Xca85III,
CCCGGG
2, 5
m4C
CCCGGGATGNNNNNNNNN[NNNN]

G
M.XcyI, M.XmaI, M.XveII

FokI
GGAT
M.CfrBI, M.Sen1735IV, M.SenAnaIV
CCWWGG
1, 6
m4C
CCWWGGGATGNNNNNNNNN[NNNN],

G

CCWWGGATGNNNNNNNNN[NNNN]

FokI
GGAT
M.Cmu51329I, M.EcoJA03PDcm,
CCWGG
1, 5
Unknown
CCWGGGATGNNNNNNNNN[NNNN],

G
M.RorR1Dcm, M.Sen8391Dcm, M.ZalSM2II

CCWGGATGNNNNNNNNN[NNNN]

FokI
GGAT
M.CocIII, M.Fsi4947II, M.TdeIII,
GGNCC
1, 5
m4C
GGATGGNCCNNNNN[NNNN]

G
M.Tph12270I

FokI
GGAT
M.Cpa18696III, M.Ype19I, M.YpeDodI,
CCWGG
2, 4
Unknown
CCWGGATGNNNNNNNNN[NNNN]

G
M.YpeEDI, M.YpeJ9I, M.YpePGI

FokI
GGAT
M.CviRI, M.Hpy298VIII, M.Hpy299VIII,
TGCA
1, 4
m6A
GGATGCANNNNNNN[NNNN]

G
M.Hpy30VI, M.Hpy32V, M.Hpy57II,

M.Hpy66VIII, M.HpyFVI, M.HpyUM032VIII,

M.HpyUM037VIII

FokI
GGAT
M.Dac11109II, M.Hfo6591I, M.MhuII,
GTAC
1, 4
m4C
GGATGTACNNNNNN[NNNN]

G
M.MjaV, M.MlaZII, M.MspLW5I, M.RsaI

FokI
GGAT
M.DdeI
CTNAG
1, 5
m5C
CTNAGGATGNNNNNNNNN[NNNN]

G

FokI
GGAT
M.DsaV, M.Ecl18kI, M.FpsJVI, M.NlaX,
CCNGG
2, 4
m5C
CCNGGATGNNNNNNNNN[NNNN]

G
M.SenPI, M.SsoII

FokI
GGAT
M.Eco29kI, M.NgoAIII, M.SacII
CCGCGG
2, 5
m5C
CCGCGGATGNNNNNNNNN[NNNN]

G

FokI
GGAT
M.EcoNI
CCTNNNN
2, 10
m4C
CCTNNNNNAGGATGNNNNNNNNN[NNNN]

G

NAGG

FokI
GGAT
M.EfaRFII, M.Mae7806II
CCGG
2, 3
m4C
CCGGATGNNNNNNNNN[NNNN]

G

FokI
GGAT
M.Esp1396I
CCANNNN
3, 9
m6A
CCANNGGATGGNNNNNNNN[NNNN]

G

NTGG

FokI
GGAT
M.FatI
CATG
1, 4
m5C
CATGGATGNNNNNNNNN[NNNN]

G

FokI
GGAT
M.FisAW1I, M.ThaI, M.Tph12270II
CGCG
1, 4
m4C
CGCGGATGNNNNNNNNN[NNNN]

G

FokI
GGAT
M.FnoB1II, M.Hal24586I, M.Hpy298V,
CCGG
1, 4
m4C
CCGGGATGNNNNNNNNN[NNNN],

G
M.Hpy299IX, M.Hpy30V, M.Hpy32XI,

CCGGATGNNNNNNNNN[NNNN]

M.Hpy57V, M.Hpy66IX, M.Hpy99VIII,

M.HpyFV, M.HpyUM032IX, M.HpyUM037IX,

M.MjaVI, M.PspJDRI, M.TmeBII

FokI
GGAT
M.FpsJI, M.HpaII, M.Nme18II, M.NmeAI
CCGG
2, 3
m5C
CCGGATGNNNNNNNNN[NNNN]

G

FokI
GGAT
M.Gel16401IV
CTGG
3
m5C
CTGGATGNNNNNNNNN[NNNN]

G

FokI
GGAT
M.Hpy188I, M.Hpy298IX, M.Hpy299V,
TCNGA
1, 5
m6A
TCGGATGNNNNNNNNN[NNNN]

G
M.Hpy66V, M.HpyUM032V

FokI
GGAT
M.Hpy30VIII, M.Hpy32VIII, M.Hpy57III,
TCNNGA
1, 6
m6A
TCNGGATGNNNNNNNNN[NNNN]

G
M.Hpy66VI, M.Hpy99XVIII, M.HpyFVIII,

M.HpyPVIII

FokI
GGAT
M.Hpy30X, M.Hpy57VIII, M.HpyFXI, M.MhuI
CTNAG
1, 5
m4C
CTNAGGATGNNNNNNNNN[NNNN]

G

FokI
GGAT
M.Hpy99IV, M.HpyFORFX
CCNNGG
1, 6
m4C
CCNNGGGATGNNNNNNNNN[NNNN],

G

CCNNGGATGNNNNNNNNN[NNNN]

FokI
GGAT
M.HpyAV, M.Nme18IV
GAAGG
3, 4
m6A,
GAAGGATGNNNNNNNNN[NNNN]

G

m5C

FokI
GGAT
M.HpyD27I
GAGG
2, 4
m6A,
GAGGGATGNNNNNNNNN[NNNN],

G

m5C
GAGGATGNNNNNNNNN[NNNN]

FokI
GGAT
M.MjaII
GGNCC
1, 5
m5C
GGATGGNCCNNNNN[NNNN]

G

FokI
GGAT
M.Msp1II
CCAGG
5
m4C
CCAGGGATGNNNNNNNNN[NNNN],

G

CCAGGATGNNNNNNNNN[NNNN]

FokI
GGAT
M.Msp42II
CCWGG
1, 5
m4C
CCWGGGATGNNNNNNNNN[NNNN],

G

CCWGGATGNNNNNNNNN[NNNN]

FokI
GGAT
M.NheI
GCTAGC
1, 6
m4C
GGATGCTAGCNNNN[NNNN]

G

FokI
GGAT
M.Nme18III
TCTGG
2, 4
m5C
TCTGGATGNNNNNNNNN[NNNN]

G

FokI
GGAT
M.NmeDI
RCCGGB
2, 5
m5C
RCCGGGATGNNNNNNNNN[NNNN]

G

FokI
GGAT
M.NmeMC58III
TCTGG
5
m5C
TCTGGGATGNNNNNNNNN[NNNN],

G

TCTGGATGNNNNNNNNN[NNNN]

FokI
GGAT
M.Nsp209III, M.Nsp51109II
CTCGAG
1, 6
m5C
CTCGAGGATGNNNNNNNNN[NNNN]

G

FokI
GGAT
M.Pam7686I
CCATGG
1, 6
m5C
CCATGGGATGNNNNNNNNN[NNNN],

G

CCATGGATGNNNNNNNNN[NNNN]

FokI
GGAT
M.Pfa23572II
CGATCG
1, 6
m5C
CGATCGGATGNNNNNNNNN[NNNN]

G

FokI
GGAT
M.Pla5I
CTAG
1, 4
Unknown
CTAGGATGNNNNNNNNN[NNNN]

G

FokI
GGAT
M.Sam53668II
CCATGG
1, 6
Unknown
CCATGGGATGNNNNNNNNN[NNNN],

G

CCATGGATGNNNNNNNNN[NNNN]

FokI
GGAT
M.Sen1781III
CCWWGG
1, 6
Unknown
CCWWGGGATGNNNNNNNNN[NNNN],

G

CCWWGGATGNNNNNNNNN[NNNN]

FokI
GGAT
M.SonIII
GACNNNN
2, 8
m6A
GACNGGATGCNNNNNNNN[NNNN]

G

TGC

FokI
GGAT
M.TdeII
GAAGAG
5, 6
m6A,
GAAGAGGATGNNNNNNNNN[NNNN]

G

m4C

FokI
GGAT
M.TspRI
CASTG
1, 5
m5C
CASTGGATGNNNNNNNNN[NNNN]

G

FokI
GGAT
M.XcmI
CCANNNN
3, 13
m6A
CCANNNNNNGGATGGNNNNNNNN[NNNN]

G

NNNNNTG

G

FokI
GGAT
M1.AgoG2I, M2.AgoG2I
ACTGG
2, 5
m4C
ACTGGGATGNNNNNNNNN[NNNN],

G

ACTGGATGNNNNNNNNN[NNNN]

FokI
GGAT
M1.Bag18758II, M2.Bag18758II
CACGGG
3, 5
Unknown
CACGGGATGNNNNNNNNN[NNNN]

G

FokI
GGAT
M1.BccI, M1.Hpy30XI, M2.Hpy66X,
GATGG
2
m6A
GGATGGNNNNNNNN[NNNN]

G
M1.Jsp2502I, M2.MmyCVI

FokI
GGAT
M1.BcnI, M2.BcnI, M.NciI
CCSGG
2, 4
m4C
CCSGGATGNNNNNNNNN[NNNN]

G

FokI
GGAT
M1.BfiI
ACTGGG
5
m4C
ACTGGGATGNNNNNNNNN[NNNN]

G

FokI
GGAT
M1.BhaI, M1.Bst19I, M.Fnu10562III,
GATGC
3
m6A
GGATGCNNNNNNNN[NNNN]

G
M1.Mbo45IV, M2.Mbo45IV

FokI
GGAT
M1.BspACI
GCGG
4
m5C
GCGGGATGNNNNNNNNN[NNNN],

G

GCGGATGNNNNNNNNN[NNNN]

FokI
GGAT
M1.Csp16704IV, M2.Csp16704IV,
GATGC
2, 3
m6A
GGATGCNNNNNNNN[NNNN]

G
M.Lsp10393I, M.Mha183II, M.Mha185II,

M.Mha807II, M.Mva1312II

FokI
GGAT
M1.Hpy299X, M2.Hpy32XIII, M1.HpyFXIII,
GATGG
2, 3
m6A
GGATGGNNNNNNNN[NNNN]

G
M2.HpyFXIII, M2.HpyUM037VI, M1.McaCI,

M2.McaCI, M1.PspHUN102II,

M2.PspHUN102II

FokI
GGAT
M1.Hpy32II
GRRGA
5
m6A
GRGGATGNNNNNNNNN[NNNN]

G

FokI
GGAT
M1.LplAG30I
GAAGG
5
m5C
GAAGGGATGNNNNNNNNN[NNNN],

G

GAAGGATGNNNNNNNNN[NNNN]

FokI
GGAT
M2.BccI, M2.Hpy299X, M1.Hpy66X,
GATGG
3
m6A
GGATGGNNNNNNNN[NNNN]

G
M1.HpyC1I, M2.HpyC1I, M1.HpyUM032X,

M2.Jsp2502I, M1.MmyCVI

FokI
GGAT
M2.BhaI
GATGC
2
m6A
GGATGCNNNNNNNN[NNNN]

G

FokI
GGAT
M2.HpyAVI, M1.MnlI
GAGG
4
m5C
GAGGGATGNNNNNNNNN[NNNN],

G

GAGGATGNNNNNNNNN[NNNN]

StsI
GGAT
M.AbaBs12I, M.Awo1030I, M.Cdu23823I,
CCWGG
1, 5
m5C
CCWGGGATGNNNNNNNNNN[NNNN],

G
M.Csa8155II, M.Cun9529I, M.EasL1Dcm,

CCWGGATGNNNNNNNNNN[NNNN]

M.Eba57Dcm, M.Ec134399Dcm,

M.Eco12761Dcm, M.Eco3609Dcm,

M.Eco4255Dcm, M.EcoVR50Dcm,

M.EsaSP1II, M.Kpn34618Dcm,

M.Kpn38547Dcm, M.Kpn39I, M.Kpn43816I,

M.Kpn677ORFDcm, M.Kpn928Dcm,

M.KpnNIH1Dcm, M.KpnNIH10Dcm,

M.KpnNIH30Dcm, M.Kva120Dcm, M.Lsp48I,

M.Pmi1I, M.SpaD2III, M.SplRp8II,

M.SptADcm, M.Tsu2489III

StsI
GGAT
M.AciI
GCGG
2, 4
m5C
GCGGGATGNNNNNNNNNN[NNNN],

G

GCGGATGNNNNNNNNNN[NNNN]

StsI
GGAT
M.Ama55I, M.CnaPQ2III, M.Rmo1926II,
CGGCCG
1, 6
m5C
CGGCCGGATGNNNNNNNNNN[NNNN]

G
M.SenC1765IV, M.SspG1I, M.XorPXII

StsI
GGAT
M.AquI
CYCGRG
1, 6
m5C
CYCGRGGATGNNNNNNNNNN[NNNN],

G

CYCGGGATGNNNNNNNNNN[NNNN]

StsI
GGAT
M.AspU41II, M.BisIII, M.BsuW23II,
CCWGG
2, 4
m5C
CCWGGATGNNNNNNNNNN[NNNN]

G
M.CnaPQ2II, M.EcoO6Dcm, M.Eco12581Dcm,

M.Eco1655Dcm, M.Eco29787Dcm,

M.Eco3024Dcm, M.Eco3317Dcm,

M.Eco3325Dcm, M.Eco3740Dcm,

M.Eco3911Dcm, M.Eco4211Dcm,

M.Eco4465Dcm, M.Eco600Dcm,

M.Eco644Dcm, M.Eco86Dcm,

M.Eco9001Dcm, M.Eco9387Dcm,

M.EcoE1140Dcm, M.EcoE455Dcm,

M.EcoGDcm, M.EcoNU14Dcm, M.EcoRII,

M.Kpn223III, M.Kpn35657II, M.Kpn36Dcm,

M.Kpn555I, M.Kpn62629II, M.KpnAATV,

M.Kqu603Dcm, M.Psp345I, M.Sen158Dcm,

M.Sen641Dcm, M.SenA46Dcm,

M.SenAboDcm, M.TcoKWC4II

StsI
GGAT
M.AvaV, M.SliSI
GATC
1, 4
m4C
GGATGATCNNNNNNN[NNNN]

G

StsI
GGAT
M.BceSII
GGWCC
1, 5
m5C
GGATGGWCCNNNNNN[NNNN]

G

StsI
GGAT
M.BcoSlacII, M.MspLW5III, M.Nbr128I,
CTCGAG
1, 6
m4C
CTCGAGGATGNNNNNNNNNN[NNNN]

G
M.Psy18884II, M.Tam77409II, M.TbrGE1II,

M.TpaRLII

StsI
GGAT
M.BepI, M.FpsJV, M.Gel16401III, M.Pdi8503I
CGCG
1, 4
m5C
CGCGGATGNNNNNNNNNN[NNNN]

G

StsI
GGAT
M.BfaSIII, M.Ccl10114I, M.CfrB38I,
ATGCAT
2, 5
m6A
GGATGCATNNNNNNN[NNNN]

G
M.CfrH1I, M.EcoGVI, M.EcoKII,

M.Fnu10562II, M.SbaUIV, M.Sbo12419III,

M.Sbo268II, M.Sen10384II, M.Sen10708II,

M.Sen1080II, M.Sen1175III, M.Sen13IV,

M.Sen13311IV, M.Sen1387II, M.Sen1427III,

M.Sen14882III, M.Sen15791III, M.Sen158III,

M.Sen16I, M.Sen1655III, M.Sen1676III,

M.Sen1677III, M.Sen1728III, M.Sen1735III,

M.Sen1736IV, M.Sen1764III, M.Sen1766III,

M.Sen1781IV, M.Sen1783III, M.Sen18569II,

M.Sen1878III, M.Sen1880III, M.Sen1896III,

M.Sen1898III, M.Sen1899III, M.Sen1903III,

M.Sen1906III, M.Sen1908III, M.Sen1910III,

M.Sen1921III, M.Sen1927II, M.Sen195II,

M.Sen2050II, M.Sen2064III, M.Sen2069III,

M.Sen22462I, M.Sen255II, M.Sen318III,

M.Sen3387III, M.Sen3890III, M.Sen483III,

M.Sen624III, M.Sen641III, M.Sen7378II,

M.Sen8391III, M.Sen8692III, M.SenA46III,

M.SenAbaII, M.SenAboII, M.SenAnaIII,

M.SenC1736III, M.SenC1765III,

M.SenC1808IV, M.SenC1810III, M.SenJIII,

M.SenL1II, M.SenL15III, M.SenN1660II,

M.SenSARA26II, M.SenSPBIII, M.SenTFIII

StsI
GGAT
M.Bhy27164IV, M.BstNI, M.EcoDHB4Dcm,
CCWGG
2, 4
m4C
CCWGGATGNNNNNNNNNN[NNNN]

G
M.EcoK54Dcm, M.Gth3570I, M.Hla49239I,

M.MvaI, M.Pdi8503II, M.Sso30807Dcm,

M.YpeI, M.Ype41I, M.YpeShI

StsI
GGAT
M.BsoBI
CYCGRG
1, 6
m4C
CYCGRGGATGNNNNNNNNNN[NNNN],

G

CYCGGGATGNNNNNNNNNN[NNNN]

StsI
GGAT
M.Bsp14III, M.Bsp3003I, M.Bsp3004I,
GATC
1, 4
m5C
GGATGATCNNNNNNN[NNNN]

G
M.CalB3III, M.CpeAIII, M.Esu35585I,

M.FpsJIV, M.Lba2031I, M.Lbo2004I,

M.LlaKR2I, M.Lmo1042I, M.Lmo7956II,

M.Lsp10393II, M.MspI7I, M.Sau3AI, M.Sba3I,

M.Sin395I, M.Spn23FI, M.Sth368I

StsI
GGAT
M.BstXI
CCANNNN
3, 10
m6A
CCANNNGGATGGNNNNNNNNN[NNNN]

G

NNTGG

StsI
GGAT
M.BsuFI, M.CpeAIV, M.MspI
CCGG
1, 4
m5C
CCGGGATGNNNNNNNNNN[NNNN],

G

CCGGATGNNNNNNNNNN[NNNN]

StsI
GGAT
M.Cab13497I, M.Dac11109III, M.Dco13527I,
CTAG
1, 4
m4C
CTAGGATGNNNNNNNNNN[NNNN]

G
M.Djo10085I, M.Hbo11551I, M.Hka19301III,

M.Hme33500I, M.HsaR1I, M.Hsi33800I,

M.HspKM1WII, M.HspNII, M.HtuI, M.HvoDSI,

M.Lba2029II, M.Mbo45II, M.MjaI, M.MthZI,

M.Nas12278I, M.Rfl3010I, M.SsmMI

StsI
GGAT
M.CagVI
TCCGGA
1, 6
m6A
TCCGGATGNNNNNNNNNN[NNNN]

G

StsI
GGAT
M.Cdi630III
CCSSGG
2, 5
m4C
CCSSGGATGNNNNNNNNNN[NNNN]

G

StsI
GGAT
M.Cfr9I, M.SmaI, M.Sma36365II, M.Xca85III,
CCCGGG
2, 5
m4C
CCCGGGATGNNNNNNNNNN[NNNN]

G
M.XcyI, M.XmaI, M.XveII

StsI
GGAT
M.CfrBI, M.Sen1735IV, M.SenAnaIV
CCWWGG
1, 6
m4C
CCWWGGGATGNNNNNNNNNN[NNNN],

G

CCWWGGATGNNNNNNNNNN[NNNN]

StsI
GGAT
M.Cmu51329I, M.EcoJA03PDcm,
CCWGG
1, 5
Unknown
CCWGGGATGNNNNNNNNNN[NNNN],

G
M.RorR1Dcm, M.Sen8391Dcm, M.ZalSM2II

CCWGGATGNNNNNNNNNN[NNNN]

StsI
GGAT
M.CocIII, M.Fsi4947II, M.TdeIII,
GGNCC
1, 5
m4C
GGATGGNCCNNNNNN[NNNN]

G
M.Tph12270I

StsI
GGAT
M.Cpa18696III, M.Ype19I, M.YpeDodI,
CCWGG
2, 4
Unknown
CCWGGATGNNNNNNNNNN[NNNN]

G
M.YpeEDI, M.YpeJ9I, M.YpePGI

StsI
GGAT
M.CviRI, M.Hpy298VIII, M.Hpy299VIII,
TGCA
1, 4
m6A
GGATGCANNNNNNNN[NNNN]

G
M.Hpy30VI, M.Hpy32V, M.Hpy57II,

M.Hpy66VIII, M.HpyFVI, M.HpyUM032VIII,

M.HpyUM037VIII

StsI
GGAT
M.Dac11109II, M.Hfo6591I, M.MhuII,
GTAC
1, 4
m4C
GGATGTACNNNNNNN[NNNN]

G
M.MjaV, M.MlaZII, M.MspLW5I, M.RsaI

StsI
GGAT
M.DdeI
CTNAG
1, 5
m5C
CTNAGGATGNNNNNNNNNN[NNNN]

G

StsI
GGAT
M.DsaV, M.Ecl18kI, M.FpsJVI, M.NlaX,
CCNGG
2, 4
m5C
CCNGGATGNNNNNNNNNN[NNNN]

G
M.SenPI, M.SsoII

StsI
GGAT
M.Eco29kI, M.NgoAIII, M.SacII
CCGCGG
2, 5
m5C
CCGCGGATGNNNNNNNNNN[NNNN]

G

StsI
GGAT
M.EcoNI
CCTNNNN
2, 10
m4C
CCTNNNNNAGGATGNNNNNNNNNN[NNNN]

G

NAGG

StsI
GGAT
M.EfaRFII, M.Mae7806II
CCGG
2, 3
m4C
CCGGATGNNNNNNNNNN[NNNN]

G

StsI
GGAT
M.Esp1396I
CCANNNN
3, 9
m6A
CCANNGGATGGNNNNNNNNN[NNNN]

G

NTGG

StsI
GGAT
M.FatI
CATG
1, 4
m5C
CATGGATGNNNNNNNNNN[NNNN]

G

StsI
GGAT
M.FisAW1I, M.ThaI, M.Tph12270II
CGCG
1, 4
m4C
CGCGGATGNNNNNNNNNN[NNNN]

G

StsI
GGAT
M.FnoB1II, M.Hal24586I, M.Hpy298V,
CCGG
1, 4
m4C
CCGGGATGNNNNNNNNNN[NNNN],

G
M.Hpy299IX, M.Hpy30V, M.Hpy32XI,

CCGGATGNNNNNNNNNN[NNNN]

M.Hpy57V, M.Hpy66IX, M.Hpy99VIII,

M.HpyFV, M.HpyUM032IX, M.HpyUM037IX,

M.MjaVI, M.PspJDRI, M.TmeBII

StsI
GGAT
M.FpsJI, M.HpaII, M.Nme18II, M.NmeAI
CCGG
2, 3
m5C
CCGGATGNNNNNNNNNN[NNNN]

G

StsI
GGAT
M.Gel16401IV
CTGG
3
m5C
CTGGATGNNNNNNNNNN[NNNN]

G

StsI
GGAT
M.Hpy188I, M.Hpy298IX, M.Hpy299V,
TCNGA
1, 5
m6A
TCGGATGNNNNNNNNNN[NNNN]

G
M.Hpy66V, M.HpyUM032V

StsI
GGAT
M.Hpy30VIII, M.Hpy32VIII, M.Hpy57III,
TCNNGA
1, 6
m6A
TCNGGATGNNNNNNNNNN[NNNN]

G
M.Hpy66VI, M.Hpy99XVIII, M.HpyFVIII,

M.HpyPVIII

StsI
GGAT
M.Hpy30X, M.Hpy57VIII, M.HpyFXI, M.MhuI
CTNAG
1, 5
m4C
CTNAGGATGNNNNNNNNNN[NNNN]

G

StsI
GGAT
M.Hpy99IV, M.HpyFORFX
CCNNGG
1, 6
m4C
CCNNGGGATGNNNNNNNNNN[NNNN],

G

CCNNGGATGNNNNNNNNNN[NNNN]

StsI
GGAT
M.HpyAV, M.Nme18IV
GAAGG
3, 4
m6A,
GAAGGATGNNNNNNNNNN[NNNN]

G

m5C

StsI
GGAT
M.HpyD27I
GAGG
2, 4
m6A,
GAGGGATGNNNNNNNNNN[NNNN],

G

m5C
GAGGATGNNNNNNNNNN[NNNN]

StsI
GGAT
M.MjaII
GGNCC
1, 5
m5C
GGATGGNCCNNNNNN[NNNN]

G

StsI
GGAT
M.Msp1II
CCAGG
5
m4C
CCAGGGATGNNNNNNNNNN[NNNN],

G

CCAGGATGNNNNNNNNNN[NNNN]

StsI
GGAT
M.Msp42II
CCWGG
1, 5
m4C
CCWGGGATGNNNNNNNNNN[NNNN],

G

CCWGGATGNNNNNNNNNN[NNNN]

StsI
GGAT
M.NheI
GCTAGC
1, 6
m4C
GGATGCTAGCNNNNN[NNNN]

G

Stsl
GGAT
M.Nme18III
TCTGG
2, 4
m5C
TCTGGATGNNNNNNNNNN[NNNN]

G

StsI
GGAT
M.NmeDI
RCCGGB
2, 5
m5C
RCCGGGATGNNNNNNNNNN[NNNN]

G

StsI
GGAT
M.NmeMC58III
TCTGG
5
m5C
TCTGGGATGNNNNNNNNNN[NNNN],

G

TCTGGATGNNNNNNNNNN[NNNN]

StsI
GGAT
M.Nsp209III, M.Nsp51109II
CTCGAG
1, 6
m5C
CTCGAGGATGNNNNNNNNNN[NNNN]

G

StsI
GGAT
M.Pam7686I
CCATGG
1, 6
m5C
CCATGGGATGNNNNNNNNNN[NNNN],

G

CCATGGATGNNNNNNNNNN[NNNN]

StsI
GGAT
M.Pfa23572II
CGATCG
1, 6
m5C
CGATCGGATGNNNNNNNNNN[NNNN]

G

StsI
GGAT
M.Pla5I
CTAG
1, 4
Unknown
CTAGGATGNNNNNNNNNN[NNNN]

G

StsI
GGAT
M.Sam53668II
CCATGG
1, 6
Unknown
CCATGGGATGNNNNNNNNNN[NNNN],

G

CCATGGATGNNNNNNNNNN[NNNN]

StsI
GGAT
M.Sen1781III
CCWWGG
1, 6
Unknown
CCWWGGGATGNNNNNNNNNN[NNNN],

G

CCWWGGATGNNNNNNNNNN[NNNN]

StsI
GGAT
M.SonIII
GACNNNN
2, 8
m6A
GACNGGATGCNNNNNNNNN[NNNN]

G

TGC

StsI
GGAT
M.TdeII
GAAGAG
5, 6
m6A,
GAAGAGGATGNNNNNNNNNN[NNNN]

G

m4C

StsI
GGAT
M.TspRI
CASTG
1, 5
m5C
CASTGGATGNNNNNNNNNN[NNNN]

G

StsI
GGAT
M.XcmI
CCANNNN
3, 13
m6A
CCANNNNNNGGATGGNNNNNNNNN[NNNN]

G

NNNNNTG

G

StsI
GGAT
M1.AgoG2I, M2.AgoG2I
ACTGG
2, 5
m4C
ACTGGGATGNNNNNNNNNN[NNNN],

G

ACTGGATGNNNNNNNNNN[NNNN]

StsI
GGAT
M1.Bag18758II, M2.Bag18758II
CACGGG
3, 5
Unknown
CACGGGATGNNNNNNNNNN[NNNN]

G

StsI
GGAT
M1.BccI, M1.Hpy30XI, M2.Hpy66X,
GATGG
2
m6A
GGATGGNNNNNNNNN[NNNN]

G
M1.Jsp2502I, M2.MmyCVI

StsI
GGAT
M1.BcnI, M2.BcnI, M.NciI
CCSGG
2, 4
m4C
CCSGGATGNNNNNNNNNN[NNNN]

G

StsI
GGAT
M1.BfiI
ACTGGG
5
m4C
ACTGGGATGNNNNNNNNNN[NNNN]

G

StsI
GGAT
M1.BhaI, M1.Bst19I, M.Fnu10562III,
GATGC
3
m6A
GGATGCNNNNNNNNN[NNNN]

G
M1.Mbo45IV, M2.Mbo45IV

StsI
GGAT
M1.BspACI
GCGG
4
m5C
GCGGGATGNNNNNNNNNN[NNNN],

G

GCGGATGNNNNNNNNNN[NNNN]

StsI
GGAT
M1.Csp16704IV, M2.Csp16704IV,
GATGC
2, 3
m6A
GGATGCNNNNNNNNN[NNNN]

G
M.Lsp10393I, M.Mha183II, M.Mha185II,

M.Mha807II, M.Mva1312II

StsI
GGAT
M1.Hpy299X, M2.Hpy32XIII, M1.HpyFXIII,
GATGG
2, 3
m6A
GGATGGNNNNNNNNN[NNNN]

G
M2.HpyFXIII, M2.HpyUM037VI, M1.McaCI,

M2.McaCI, M1.PspHUN102II,

M2.PspHUN102II

StsI
GGAT
M1.Hpy32II
GRRGA
5
m6A
GRGGATGNNNNNNNNNN[NNNN]

G

StsI
GGAT
M1.LplAG30I
GAAGG
5
m5C
GAAGGGATGNNNNNNNNNN[NNNN],

G

GAAGGATGNNNNNNNNNN[NNNN]

StsI
GGAT
M2.BccI, M2.Hpy299X, M1.Hpy66X,
GATGG
3
m6A
GGATGGNNNNNNNNN[NNNN]

G
M1.HpyC1I, M2.HpyC1I, M1.HpyUM032X,

M2.Jsp2502I, M1.MmyCVI

StsI
GGAT
M2.BhaI
GATGC
2
m6A
GGATGCNNNNNNNNN[NNNN]

G

StsI
GGAT
M2.HpyAVI, M1.MnlI
GAGG
4
m5C
GAGGGATGNNNNNNNNNN[NNNN],

G

GAGGATGNNNNNNNNNN[NNNN]

Acc36I, BfuAI,
ACCT
M.AauTCI, M.AchA6II, M.Asp146I,
TTAA
1, 4
m6A
TTAACCTGCNNNN[NNNN]

BspMI, BveI
GC
M.Asp24188I, M.Asp31YII, M.AspU41I,

M.BspJKG1III, M.EsaDix4I, M.EsaDix5I,

M.Llu27648II, M.Mch9957II, M.Mru1279VI,

M.MseI, M.Msi9946I, M.Sam53668III,

M.Sth20745I, M.Tam 77409I

Acc36I, BfuAI,
ACCT
M.AbaBs12I, M.Awo1030I, M.Cdu23823I,
CCWGG
1, 5
m5C
ACCTGCCWGG[NNNN]

BspMI, BveI
GC
M.Csa8155II, M.Cun9529I, M.EasL1Dcm,

M.Eba57Dcm, M.Ecl34399Dcm,

M.Eco12761Dcm, M.Eco3609Dcm,

M.Eco4255Dcm, M.EcoVR50Dcm,

M.EsaSP1II, M.Kpn34618Dcm,

M.Kpn38547Dcm, M.Kpn39I, M.Kpn43816I,

M.Kpn677ORFDcm, M.Kpn928Dcm,

M.KpnNIH1Dcm, M.KpnNIH10Dcm,

M.KpnNIH30Dcm, M.Kva120Dcm, M.Lsp48I,

M.Pmi1I, M.SpaD2III, M.SplRp8II,

M.SptADcm, M.Tsu2489III

Acc36I, BfuAI,
ACCT
M.Acc65I, M.Eco86I, M.KpnI, M.Lal216III,
GGTACC
3, 4
m6A
GGTACCTGCNNNN[NNNN]

BspMI, BveI
GC
M.Sen3387IV, M.Sen8391IV

Acc36I, BfuAI,
ACCT
M.Acc65VI, M.SenAZIII
CTKMAG
2, 5
m6A
ACCTGCAGNN[NNNN]

BspMI, BveI
GC

Acc36I, BfuAI,
ACCT
M.AccI, M.FpsFPG3II, M.SdeAIV
GTMKAC
2, 5
m6A
GTMKACCTGCNNNN[NNNN]

BspMI, BveI
GC

Acc36I, BfuAI,
ACCT
M.AciI
CCGC
1, 3
m5C
ACCTGCGGNN[NNNN],

BspMI, BveI
GC

ACCTGCCGCN[NNNN]

Acc36I, BfuAI,
ACCT
M.Ade56712I, M.Asp588III, M.AveCB51II,
CTGCAG
2, 5
m6A
ACCTGCAGNN[NNNN]

BspMI, BveI
GC
M.BbrUIII, M.Blo30698I, M.BsuBI,

M.CpaYL7III, M.Dba7044I, M.Dfa45958II,

M.Eco12581I, M.Eco2012I, M.EcoGIII,

M.EcoG089I, M.Eho16691II, M.Hal5920II,

M.Lsp6406II, M.MspNR41II, M.Pae490I,

M.Pox23570I, M.PstI, M.RpaII, M.Sca3403II,

M.Tsp61I, M.XmnII, M.YenWI, M.YinY228I

Acc36I, BfuAI,
ACCT
M.AgsI
TTSAA
1, 5
m6A
TTSAACCTGCNNNN[NNNN]

BspMI, BveI
GC

Acc36I, BfuAI,
ACCT
M.AhdI
GACNNNN
2, 10
m6A
GACCTGCNGTC[NNNN]

BspMI, BveI
GC

NGTC

Acc36I, BfuAI,
ACCT
M.Alw26I
GAGAC
4, 3
m6A,
GAGACCTGCNNNN[NNNN]

BspMI, BveI
GC

m5C

Acc36I, BfuAI,
ACCT
M.AorT14III, M.GsuRed1III, M.SalI
GTCGAC
2, 5
m6A
GTCGACCTGCNNNN[NNNN]

BspMI, BveI
GC

Acc36I, BfuAI,
ACCT
M.AplI
CTGCAG
3, 4
m5C
ACCTGCAGNN[NNNN]

BspMI, BveI
GC

Acc36I, BfuAI,
ACCT
M.AvaIX, M.Cfr10I, M.Nme18VI, M.VchAI,
RCCGGY
2, 5
m5C
ACCTGCCGGY [NNNN]

BspMI, BveI
GC
M.Vch0395I

Acc36I, BfuAI,
ACCT
M.BamWS8I, M.CthV, M.EfaRFI, M.Saf8902II,
GCWGC
2, 4
m5C
ACCTGCWGCN[NNNN]

BspMI, BveI
GC
M.TacII, M.TcoKWC4III

Acc36I, BfuAI,
ACCT
M.BanLII, M.CpeAII, M.SinI
GGWCC
2, 4
m5C
GGACCTGCNNNN[NNNN]

BspMI, BveI
GC

Acc36I, BfuAI,
ACCT
M.BbvI, M.BceSIV
GCAGC
2, 4
m5C
ACCTGCTGCN[NNNN],

BspMI, BveI
GC

ACCTGCAGCN[NNNN]

Acc36I, BfuAI,
ACCT
M.Bce16656II, M.Bce22E1II, M.Bce25416II,
GTWWAC
2, 5
m6A
GTWWACCTGCNNNN[NNNN]

BspMI, BveI
GC
M.Bce842II, M.Bce895II, M.BceJIV,

M.BceK56II, M.Bgl2196I, M.BmaBMKII,

M.BmaBMZII, M.Bps10134II, M.Bps1651II,

M.Bps7894II, M.Bps840III, M.BpsR668II,

M.BspRB39I, M.Bth1643II, M.Cba4G11II,

M.MspNR41I, M.Oba14I, M.Pap16535I,

M.Pfa23572I, M.Pox23570II, M.PpnI,

M.PpnRB38I, M.Pth25325I

Acc36I, BfuAI,
ACCT
M.BceSII
GGWCC
1, 5
m5C
GGACCTGCNNNN[NNNN]

BspMI, BveI
GC

Acc36I, BfuAI,
ACCT
M.BepI, M.FpsJV, M.Gel16401III, M.Pdi8503I
CGCG
1, 4
m5C
ACCTGCGCGN[NNNN]

BspMI, BveI
GC

Acc36I, BfuAI,
ACCT
M.Bhy27164V, M.DraI, M.Lsp6406V,
TTTAAA
1, 6
m6A
TTTAAACCTGCNNNN[NNNN]

BspMI, BveI
GC
M.Tsu2489V

Acc36I, BfuAI,
ACCT
M.BspRAC04I, M.EcaI, M.Eco3936IV
GGTNACC
3, 5
m6A
GGTNACCTGCNNNN[NNNN]

BspMI, BveI
GC

Acc36I, BfuAI,
ACCT
M.BstXI
CCANNNN
3, 10
m6A
CCACCTGCNTGG[NNNN]

BspMI, BveI
GC

NNTGG

Acc36I, BfuAI,
ACCT
M.BsuFI, M.CpeAIV, M.MspI
CCGG
1, 4
m5C
ACCTGCCGGN[NNNN]

BspMI, BveI
GC

Acc36I, BfuAI,
ACCT
M.Cab13497I, M.Dac11109III, M.Dco13527I,
CTAG
1, 4
m4C
ACCTGCTAGN[NNNN]

BspMI, BveI
GC
M.Djo10085I, M.Hbo11551I, M.Hka19301III,

M.Hme33500I, M.HsaR1I, M.Hsi33800I,

M.HspKM1WII, M.HspNII, M.HtuI, M.HvoDSI,

M.Lba2029II, M.Mbo45II, M.MjaI, M.MthZI,

M.Nas12278I, M.Rfl3010I, M.SsmMI

Acc36I, BfuAI,
ACCT
M.Cac8I
GCNNGC
2, 5
m5C
ACCTGCNNGC[NNNN]

BspMI, BveI
GC

Acc36I, BfuAI,
ACCT
M.CagV, M.Lsp6406I, M.Rsp7740I,
TTCGAA
1, 6
m6A
TTCGAACCTGCNNNN[NNNN]

BspMI, BveI
GC
M.RspPBTS2II, M.VbaLP2AI

Acc36I, BfuAI,
ACCT
M.CagVI
TCCGGA
1, 6
m6A
TCCGGACCTGCNNNN[NNNN]

BspMI, BveI
GC

Acc36I, BfuAI,
ACCT
M.Cdi630IV, M.Ckr177III, M.CmaLM2II,
GCNGC
2, 4
m5C
ACCTGCNGCN[NNNN]

BspMI, BveI
GC
M.CocII, M.Fnu4HI, M.Fsp4HI

Acc36I, BfuAI,
ACCT
M.Cdi630V, M.Cdi81I, M.CdiG46II,
CAAAAA
6
m6A
CAAAAACCTGCNNNN[NNNN]

BspMI, BveI
GC
M.CmaLM2I, M.CpaYL7II, M.Pat19335II,

M.PbaVA2I, M.Pdi9689I

Acc36I, BfuAI,
ACCT
M.Cmu51329I, M.EcoJA03PDcm,
CCWGG
1, 5
Unknown
ACCTGCCWGG[NNNN]

BspMI, BveI
GC
M.RorR1Dcm, M.Sen8391Dcm, M.ZalSM2II

Acc36I, BfuAI,
ACCT
M.CocI, M.Fli6794II, M.HincII, M.HindII,
GTYRAC
2, 5
m6A
GTYRACCTGCNNNN[NNNN]

BspMI, BveI
GC
M.HinHia1II

Acc36I, BfuAI,
ACCT
M.CocIII, M.Fsi4947II, M.TdeIII,
GGNCC
1, 5
m4C
GGACCTGCNNNN[NNNN]

BspMI, BveI
GC
M.Tph12270I

Acc36I, BfuAI,
ACCT
M.CpeAVI, M.TcoKWC4I
GTATAC
2, 5
m6A
GTATACCTGCNNNN[NNNN]

BspMI, BveI
GC

Acc36I, BfuAI,
ACCT
M.CsiFSMII, M.MfoCT6IV, M.NgoAIV,
GCCGGC
2, 5
m5C
ACCTGCCGGC[NNNN]

BspMI, BveI
GC
M.NgoMIV, M1.Sgr13350II

Acc36I, BfuAI,
ACCT
M.CviQI, M.Hpy32XII, M.Hpy57XI,
GTAC
2, 3
m6A
GTACCTGCNNNN[NNNN]

BspMI, BveI
GC
M.Hpy66XII, M.Hpy99XII, M.HpyFXII,

M.HpyPIV, M.HpyUM037XI, M.PabI

Acc36I, BfuAI,
ACCT
M.CviQIII, M.CviSIII, M.Hpy299XI,
TCGA
1, 4
m6A
TCGACCTGCNNNN[NNNN]

BspMI, BveI
GC
M.Hpy30II, M.Hpy32X, M.Hpy99VII,

M.HpyAX, M.HpyFII, M.HpyUM032XVII,

M.Tam77409III, M.TaqI, M.TpaRLI,

M.Tth HB8I

Acc36I, BfuAI,
ACCT
M.CviRI, M.Hpy298VIII, M.Hpy299VIII,
TGCA
1, 4
m6A
TGCACCTGCNNNN[NNNN],

BspMI, BveI
GC
M.Hpy30VI, M.Hpy32V, M.Hpy57II,

ACCTGCANNN[NNNN]

M.Hpy66VIII, M.HpyFVI, M.HpyUM032VIII,

M.HpyUM037VIII

Acc36I, BfuAI,
ACCT
M.Dac11109II, M.Hfo6591I, M.MhuII,
GTAC
1, 4
m4C
GTACCTGCNNNN[NNNN]

BspMI, BveI
GC
M.MjaV, M.MlaZII, M.MspLW5I, M.RsaI

Acc36I, BfuAI,
ACCT
M.DdeI
CTNAG
1, 5
m5C
ACCTGCTNAG[NNNN]

BspMI, BveI
GC

Acc36I, BfuAI,
ACCT
M.DfeI, M.Fnu419II, M.Pen113II
CTNNAG
2, 5
m6A
ACCTGCAGNN[NNNN]

BspMI, BveI
GC

Acc36I, BfuAI,
ACCT
M.EcoO109I
RGGNCCY
3, 5
m5C
RGGACCTGCNNNN[NNNN]

BspMI, BveI
GC

Acc36I, BfuAI,
ACCT
M.Esp1396I
CCANNNN
3, 9
m6A
CCACCTGCTGGN[NNNN]

BspMI, BveI
GC

NTGG

Acc36I, BfuAI,
ACCT
M.FatI
CATG
1, 4
m5C
ACCTGCATGN[NNNN]

BspMI, BveI
GC

Acc36I, BfuAI,
ACCT
M.FisAW1I, M.ThaI, M.Tph12270II
CGCG
1, 4
m4C
ACCTGCGCGN[NNNN]

BspMI, BveI
GC

Acc36I, BfuAI,
ACCT
M.FnoB1II, M.Hal24586I, M.Hpy298V,
CCGG
1, 4
m4C
ACCTGCCGGN[NNNN]

BspMI, BveI
GC
M.Hpy299IX, M.Hpy30V, M.Hpy32XI,

M.Hpy57V, M.Hpy66IX, M.Hpy99VIII,

M.HpyFV, M.HpyUM032IX, M.HpyUM037IX,

M.MjaVI, M.PspJDRI, M.TmeBII

Acc36I, BfuAI,
ACCT
M.HgiCI
GGYRCC
2, 5
m5C
GGYACCTGCNNNN[NNNN]

BspMI, BveI
GC

Acc36I, BfuAI,
ACCT
M.HhaI, M.HinP1I, M.Hpy99III, M.HpyAVIII,
GCGC
2, 3
m5C
ACCTGCGCNN[NNNN]

BspMI, BveI
GC
M.HpyUM032XV

Acc36I, BfuAI,
ACCT
M.Hme33500II
HGCWGCK
3
m4C
ACCTGCWGCK[NNNN]

BspMI, BveI
GC

Acc36I, BfuAI,
ACCT
M.HpaI, M.Mwh3033I, M.Rsp7I
GTTAAC
2, 5
m6A
GTTAACCTGCNNNN[NNNN]

BspMI, BveI
GC

Acc36I, BfuAI,
ACCT
M.Hpy188I, M.Hpy298IX, M.Hpy299V,
TCNGA
1, 5
m6A
TCNGACCTGCNNNN[NNNN]

BspMI, BveI
GC
M.Hpy66V, M.HpyUM032V

Acc36I, BfuAI,
ACCT
M.Hpy30IX, M.Hpy32IX, M.Hpy57IX,
GTNNAC
2, 5
m6A
GTNNACCTGCNNNN[NNNN]

BspMI, BveI
GC
M.Hpy66XIII, M.Hpy8I, M.HpyFIX,

M.HpyUM032XI, M.HpyUM037V, M.MjaIV,

M.MspRAC08II

Acc36I, BfuAI,
ACCT
M.Hpy30VIII, M.Hpy32VIII, M.Hpy57III,
TCNNGA
1, 6
m6A
TCNNGACCTGCNNNN[NNNN]

BspMI, BveI
GC
M.Hpy66VI, M.Hpy99XVIII, M.HpyFVIII,

M.HpyPVIII

Acc36I, BfuAI,
ACCT
M.Hpy30X, M.Hpy57VIII, M.HpyFXI, M.MhuI
CTNAG
1, 5
m4C
ACCTGCTNAG[NNNN]

BspMI, BveI
GC

Acc36I, BfuAI,
ACCT
M.Hpy57X
CTRYAG
2, 5
m6A
ACCTGCAGNN[NNNN]

BspMI, BveI
GC

Acc36I, BfuAI,
ACCT
M.Hpy99II, M.Tsp45I, M.Tsu2489II
GTSAC
2, 4
m6A
GTSACCTGCNNNN[NNNN]

BspMI, BveI
GC

Acc36I, BfuAI,
ACCT
M.HpyD27I
CCTC
1, 3
m5C,
ACCTGCCTCN[NNNN]

BspMI, BveI
GC

m6A

Acc36I, BfuAI,
ACCT
M.Lph145I, M.Lph93I
GGWGA
5
m6A
GGWGACCTGCNNNN[NNNN]

BspMI, BveI
GC

Acc36I, BfuAI,
ACCT
M.Mae7806III, M.PspPI, M.Sau96I
GGNCC
2, 4
m5C
GGACCTGCNNNN[NNNN]

BspMI, BveI
GC

Acc36I, BfuAI,
ACCT
M.MjaII
GGNCC
1, 5
m5C
GGACCTGCNNNN[NNNN]

BspMI, BveI
GC

Acc36I, BfuAI,
ACCT
M.Msp1II
CCTGG
1
m4C
ACCTGCCTGG[NNNN]

BspMI, BveI
GC

Acc36I, BfuAI,
ACCT
M.Msp42II
CCWGG
1, 5
m4C
ACCTGCCWGG[NNNN]

BspMI, BveI
GC

Acc36I, BfuAI,
ACCT
M.NgoAXV, M.NgoMV
GGNNCC
2, 5
m5C
GGNACCTGCNNNN[NNNN]

BspMI, BveI
GC

Acc36I, BfuAI,
ACCT
M.NheI
GCTAGC
1, 6
m4C
ACCTGCTAGC[NNNN]

BspMI, BveI
GC

Acc36I, BfuAI,
ACCT
M.Nme18V
GCGCGC
2, 5
m5C
ACCTGCGCGC[NNNN]

BspMI, BveI
GC

Acc36I, BfuAI,
ACCT
M.NmeDI
VCCGGY
2, 5
m5C
ACCTGCCGGB[NNNN]

BspMI, BveI
GC

Acc36I, BfuAI,
ACCT
M.NmeMC58III
CCAGA
1
m5C
ACCTGCCAGA[NNNN]

BspMI, BveI
GC

Acc36I, BfuAI,
ACCT
M.NspI, M.NspHI
RCATGY
2, 5
m5C
ACCTGCATGY [NNNN]

BspMI, BveI
GC

Acc36I, BfuAI,
ACCT
M.Pla5I
CTAG
1, 4
Unknown
ACCTGCTAGN[NNNN]

BspMI, BveI
GC

Acc36I, BfuAI,
ACCT
M.SmoLVI, M.Spn23FII, M.Spn556I,
TCTAGA
1, 6
m6A
TCTAGACCTGCNNNN[NNNN]

BspMI, BveI
GC
M.Spn7465I, M.SpnRI, M.XbaI

Acc36I, BfuAI,
ACCT
M.SonIII
GACNNNN
2, 8
m6A
GACCTGCTGCN[NNNN],

BspMI, BveI
GC

TGC

GACNACCTGCNNNN[NNNN]

Acc36I, BfuAI,
ACCT
M.TspRI
CASTG
1, 5
m5C
ACCTGCASTG[NNNN]

BspMI, BveI
GC

Acc36I, BfuAI,
ACCT
M.Yen2516I
GCGCGC
2, 5
Unknown
ACCTGCGCGC[NNNN]

BspMI, BveI
GC

Acc36I, BfuAI,
ACCT
M1.AgoG2I, M2.AgoG2I
CCAGT
1, 4
m4C
ACCTGCCAGT[NNNN]

BspMI, BveI
GC

Acc36I, BfuAI,
ACCT
M1.BspACI
CCGC
1
m5C
ACCTGCCGCN[NNNN]

BspMI, BveI
GC

Acc36I, BfuAI,
ACCT
M1.Eco31I, M1.EcoMI
GAGACC
4
m6A
GAGACCTGCNNNN[NNNN]

BspMI, BveI
GC

Acc36I, BfuAI,
ACCT
M1.HgaI, M2.LlaJI
GCGTC
2
m5C
ACCTGCGTCN[NNNN]

BspMI, BveI
GC

Acc36I, BfuAI,
ACCT
M1.Hpy32II
GRRGA
5
m6A
GRRGACCTGCNNNN[NNNN]

BspMI, BveI
GC

Acc36I, BfuAI,
ACCT
M1.HpyAII, M1.MboII, M1.NcuI
GAAGA
5
m6A
GAAGACCTGCNNNN[NNNN]

BspMI, BveI
GC

Acc36I, BfuAI,
ACCT
M1.LplAG30I
CCTTC
1
m5C
ACCTGCCTTC[NNNN]

BspMI, BveI
GC

Acc36I, BfuAI,
ACCT
M2.BceSIII, M1.Pru9I, M2.Pru9I
GCCGT
2, 4
m4C
ACCTGCCGTN[NNNN]

BspMI, BveI
GC

Acc36I, BfuAI,
ACCT
M2.ClaSBI, M1.Fli6794IV, M2.HphI,
GGTGA
5
m6A
GGTGACCTGCNNNN[NNNN]

BspMI, BveI
GC
M2.Ngo3502II, M1.Ngo6140II, M1.NgoAXVI,

M1.NgoBVIII, M1.NgoFA19I, M2.NgoMVIII,

M2.PspHUN102I, M1.Ssp10II

Acc36I, BfuAI,
ACCT
M2.HpyAVI, M1.MnlI
CCTC
1
m5C
ACCTGCCTCN[NNNN]

BspMI, BveI
GC

Acc36I, BfuAI,
ACCT
M2.RspA76I
GGGAC
4
m6A
GGGACCTGCNNNN[NNNN]

BspMI, BveI
GC

AceIII
CAGC
M.AbaBs12I, M.Awo1030I, M.Cdu23823I,
CCWGG
1, 5
m5C
CAGCTCCWGGNNN[NNNN]

TC
M.Csa8155II, M.Cun9529I, M.EasL1Dcm,

M.Eba57Dcm, M.Ec134399Dcm,

M.Eco12761Dcm M.Eco3609Dcm,

M.Eco4255Dcm, M.EcoVR50Dcm,

M.EsaSP1II, M.Kpn34618Dcm,

M.Kpn38547Dcm, M.Kpn39I, M.Kpn43816I,

M.Kpn677ORFDcm, M.Kpn928Dcm,

M.KpnNIH1Dcm, M.KpnNIH10Dcm,

M.KpnNIH30Dcm, M.Kva120Dcm, M.Lsp48I,

M.Pmi1I, M.SpaD2III, M.SplRp8II,

M.SptADcm, M.Tsu2489III

AceIII
CAGC
M.Acc65VI, M.SenAZIII
CTKMAG
2, 5
m6A
CTKCAGCTCNNNNNNN[NNNN]

TC

AceIII
CAGC
M.AciI
CCGC
1, 3
m5C
CAGCTCCGCNNNN[NNNN]

TC

AceIII
CAGC
M.Ade56712I, M.Asp588III, M.AveCB51II,
CTGCAG
2, 5
m6A
CTGCAGCTCNNNNNNN[NNNN]

TC
M.BbrUIII, M.Blo30698I, M.BsuBI,

M.CpaYL7III, M.Dba7044I, M.Dfa45958II,

M.Eco12581I, M.Eco2012I, M.EcoGIII,

M.EcoG089I, M.Eho16691II, M.Hal5920II,

M.Lsp6406II, M.MspNR41II, M.Pae490I,

M.Pox23570I, M.PstI, M.RpaII, M.Sca3403II,

M.Tsp61I, M.XmnII, M.YenWI, M.YinY228I

AceIII
CAGC
M.Ama55I, M.CnaPQ2III, M.Rmo1926II,
CGGCCG
1, 6
m5C
CAGCTCGGCCGNN[NNNN]

TC
M.SenC1765IV, M.SspG1I, M.XorPXII

AceIII
CAGC
M.AplI
CTGCAG
3, 4
m5C
CTGCAGCTCNNNNNNN[NNNN]

TC

AceIII
CAGC
M.AquI
CYCGRG
1, 6
m5C
CAGCTCGRGNNNN[NNNN],

TC

CAGCTCYCGRGNN[NNNN]

AceIII
CAGC
M.Asp588II, M.BstVI, M.Dsp964I, M.DvuII,
CTCGAG
2, 5
m6A
CAGCTCGAGNNNN[NNNN]

TC
M.GsuRed1II, M.Hka19301II, M.Mch9957IV,

M.MfoCT6II, M.Mhy14884I, M.Mru1279II,

M.MsmI, M.Msm8159I, M.Nal45188I,

M.Osp807I, M.Pae63I, M.PaeR7I, M.XhoI

AceIII
CAGC
M.AvaV, M.SliSI
GATC
1, 4
m4C
GATCAGCTCNNNNNNN[NNNN]

TC

AceIII
CAGC
M.BamWS8I, M.CthV, M.EfaRFI, M.Saf8902II,
GCWGC
2, 4
m5C
GCAGCTCNNNNNNN[NNNN]

TC
M.TacII, M.TcoKWC4III

AceIII
CAGC
M.BbvI, M.BceSIV
GCAGC
2, 4
m5C
GCAGCTCNNNNNNN[NNNN]

TC

AceIII
CAGC
M.BceSII
GGWCC
1, 5
m5C
GGWCCAGCTCNNNNNNN[NNNN]

TC

AceIII
CAGC
M.BcoSlacII, M.MspLW5III, M.Nbr128I,
CTCGAG
1, 6
m4C
CAGCTCGAGNNNN[NNNN],

TC
M.Psy18884II, M.Tam77409II, M.TbrGE1II,

CAGCTCTCGAGNN[NNNN]

M.TpaRLII

AceIII
CAGC
M.BepI, M.FpsJV, M.Gel16401III, M.Pdi8503I
CGCG
1, 4
m5C
CAGCTCGCGNNNN[NNNN]

TC

AceIII
CAGC
M.BsoBI
CYCGRG
1, 6
m4C
CAGCTCGRGNNNN[NNNN],

TC

CAGCTCYCGRGNN[NNNN]

AceIII
CAGC
M.Bsp14III, M.Bsp3003I, M.Bsp3004I,
GATC
1, 4
m5C
GATCAGCTCNNNNNNN[NNNN]

TC
M.CalB3III, M.CpeAIII, M.Esu35585I,

M.FpsJIV, M.Lba2031I, M.Lbo2004I,

M.LlaKR2I, M.Lmo1042I, M.Lmo7956II,

M.Lsp10393II, M.MspI7I, M.Sau3AI, M.Sba3I,

M.Sin395I, M.Spn23FI, M.Sth368I

AceIII
CAGC
M.BstXI
CCANNNN
3, 10
m6A
CCAGCTCNNTGGNN[NNNN]

TC

NNTGG

AceIII
CAGC
M.BsuFI, M.CpeAIV, M.MspI
CCGG
1, 4
m5C
CAGCTCCGGNNNN[NNNN]

TC

AceIII
CAGC
M.Cab13497I, M.Dac11109III, M.Dco13527I,
CTAG
1, 4
m4C
CAGCTCTAGNNNN[NNNN]

TC
M.Djo10085I, M.Hbo11551I, M.Hka19301III,

M.Hme33500I, M.HsaR1I, M.Hsi33800I,

M.HspKM1WII, M.HspNII, M.HtuI, M.HvoDSI,

M.Lba2029II, M.Mbo45II, M.MjaI, M.MthZI,

M.Nas12278I, M.Rfl3010I, M.SsmMI

AceIII
CAGC
M.Cac8I
GCNNGC
2, 5
m5C
GCCAGCTCNNNNNNN[NNNN],

TC

CAGCTCGCNNNNN[NNNN]

AceIII
CAGC
M.CagVI
TCCGGA
1, 6
m6A
CAGCTCCGGANNN[NNNN]

TC

AceIII
CAGC
M.Cdi630IV, M.Ckr177III, M.CmaLM2II,
GCNGC
2, 4
m5C
GCAGCTCNNNNNNN[NNNN]

TC
M.CocII, M.Fnu4HI, M.Fsp4HI

AceIII
CAGC
M.CfrBI, M.Sen1735IV, M.SenAnaIV
CCWWGG
1, 6
m4C
CAGCTCCWWGGNN[NNNN]

TC

AceIII
CAGC
M.Cmu51329I, M.EcoJA03PDcm,
CCWGG
1, 5
Unknown
CAGCTCCWGGNNN[NNNN]

TC
M.RorR1Dcm, M.Sen8391Dcm, M.ZalSM2II

AceIII
CAGC
M.CocIII, M.Fsi4947II, M.TdeIII,
GGNCC
1, 5
m4C
GGNCCAGCTCNNNNNNN[NNNN]

TC
M.Tph12270I

AceIII
CAGC
M.Cph17I, M.Cph41I
CTYCAG
5
m6A
CTYCAGCTCNNNNNNN[NNNN]

TC

AceIII
CAGC
M.CviQIII, M.CviSIII, M.Hpy299XI,
TCGA
1, 4
m6A
CAGCTCGANNNNN[NNNN]

TC
M.Hpy30II, M.Hpy32X, M.Hpy99VII,

M.HpyAX, M.HpyFII, M.HpyUM032XVII,

M.Tam77409III, M.TaqI, M.TpaRLI,

M.TthHB8I

AceIII
CAGC
M.CviRI, M.Hpy298VIII, M.Hpy299VIII,
TGCA
1, 4
m6A
TGCAGCTCNNNNNNN[NNNN]

TC
M.Hpy30VI, M.Hpy32V, M.Hpy57II,

M.Hpy66VIII, M.HpyFVI, M.HpyUM032VIII,

M.HpyUM037VIII

AceIII
CAGC
M.Dac11109II, M.Hfo6591I, M.MhuII,
GTAC
1, 4
m4C
GTACAGCTCNNNNNNN[NNNN]

TC
M.MjaV, M.MlaZII, M.MspLW5I, M.RsaI

AceIII
CAGC
M.DdeI
CTNAG
1, 5
m5C
CTCAGCTCNNNNNNN[NNNN],

TC

CAGCTCAGNNNNN[NNNN],

CAGCTCTNAGNNN[NNNN]

AceIII
CAGC
M.DfeI, M.Fnu419II, M.Pen113II
CTNNAG
2, 5
m6A
CTNCAGCTCNNNNNNN[NNNN],

TC

CAGCTCNAGNNNN[NNNN]

AceIII
CAGC
M.Esp1396I
CCANNNN
3, 9
m6A
CCAGCTCNTGGNNN[NNNN]

TC

NTGG

AceIII
CAGC
M.FatI
CATG
1, 4
m5C
CAGCTCATGNNNN[NNNN]

TC

AceIII
CAGC
M.FisAW1I, M.ThaI, M.Tph12270II
CGCG
1, 4
m4C
CAGCTCGCGNNNN[NNNN]

TC

AceIII
CAGC
M.FnoB1II, M.Hal24586I, M.Hpy298V,
CCGG
1, 4
m4C
CAGCTCCGGNNNN[NNNN]

TC
M.Hpy299IX, M.Hpy30V, M.Hpy32XI,

M.Hpy57V, M.Hpy66IX, M.Hpy99VIII,

M.HpyFV, M.HpyUM032IX, M.HpyUM037IX,

M.MjaVI, M.PspJDRI, M.TmeBII

AceIII
CAGC
M.Gel16401IV
CCAG
2
m5C
CCAGCTCNNNNNNN[NNNN]

TC

AceIII
CAGC
M.Hka19301IV
TCGCGA
2, 5
m4C
CAGCTCGCGANNN[NNNN]

TC

AceIII
CAGC
M.Hme33500II
HGCWGCK
3
m4C
HGCAGCTCNNNNNNN[NNNN]

TC

AceIII
CAGC
M.Hpy188I, M.Hpy298IX, M.Hpy299V,
TCNGA
1, 5
m6A
CAGCTCNGANNNN[NNNN]

TC
M.Hpy66V, M.HpyUM032V

AceIII
CAGC
M.Hpy30VIII, M.Hpy32VIII, M.Hpy57III,
TCNNGA
1, 6
m6A
CAGCTCNNGANNN[NNNN]

TC
M.Hpy66VI, M.Hpy99XVIII, M.HpyFVIII,

M.HpyPVIII

AceIII
CAGC
M.Hpy30X, M.Hpy57VIII, M.HpyFXI, M.MhuI
CTNAG
1, 5
m4C
CTCAGCTCNNNNNNN[NNNN],

TC

CAGCTCAGNNNNN[NNNN],

CAGCTCTNAGNNN[NNNN]

AceIII
CAGC
M.Hpy57X
CTRYAG
2, 5
m6A
CTRCAGCTCNNNNNNN[NNNN]

TC

AceIII
CAGC
M.Hpy99IV, M.HpyFORFX
CCNNGG
1, 6
m4C
CAGCTCCNNGGNN[NNNN]

TC

AceIII
CAGC
M.HpyD27I
CCTC
1, 3
m5C,
CAGCTCCTCNNNN[NNNN]

TC

m6A

AceIII
CAGC
M.Kpn555II, M.Mha183III
CTTCAG
2, 5
m6A
CTTCAGCTCNNNNNNN[NNNN]

TC

AceIII
CAGC
M.Lph145I, M.Lph93I
TCWCC
1
m6A
CAGCTCWCCNNNN[NNNN]

TC

AceIII
CAGC
M.Lph145II, M.Lph93II
CYAG
3
m6A
CCAGCTCNNNNNNN[NNNN]

TC

AceIII
CAGC
M.Lph15I
CCAG
3
m6A
CCAGCTCNNNNNNN[NNNN]

TC

AceIII
CAGC
M.Maf25III, M.Mbo26III, M.Mbo30III,
CTCCAG
2, 5
m6A
CTCCAGCTCNNNNNNN[NNNN],

TC
M.MboBCG21I, M.MkaDI, M.Mtu22103III,

CAGCTCCAGNNNN[NNNN]

M.Mtu22115II, M.Mtu26105II, M.Mtu28I,

M.Mtu37004II, M.MtuF1I, M.MtuHIII,

M.MtuJKIII

AceIII
CAGC
M.MjaII
GGNCC
1, 5
m5C
GGNCCAGCTCNNNNNNN[NNNN]

TC

AceIII
CAGC
M.Msp1II
CCTGG
1
m4C
CAGCTCCTGGNNN[NNNN]

TC

AceIII
CAGC
M.Msp42II
CCWGG
1, 5
m4C
CAGCTCCWGGNNN[NNNN]

TC

AceIII
CAGC
M.NheI
GCTAGC
1, 6
m4C
GCTAGCAGCTCNNNNNNN[NNNN]

TC

AceIII
CAGC
M.Nme18III
TCTGG
2, 4
m5C
CAGCTCTGGNNNN[NNNN]

TC

AceIII
CAGC
M.NmeMC58III
CCAGA
1
m5C
CAGCTCCAGANNN[NNNN]

TC

AceIII
CAGC
M.Nsp209III, M.Nsp51109II
CTCGAG
1, 6
m5C
CAGCTCGAGNNNN[NNNN],

TC

CAGCTCTCGAGNN[NNNN]

AceIII
CAGC
M.Pam7686I
CCATGG
1, 6
m5C
CAGCTCCATGGNN[NNNN]

TC

AceIII
CAGC
M.Pfa23572II
CGATCG
1, 6
m5C
CAGCTCGATCGNN[NNNN]

TC

AceIII
CAGC
M.Pla5I
CTAG
1, 4
Unknown
CAGCTCTAGNNNN[NNNN]

TC

AceIII
CAGC
M.RpaIV
CTCGAG
3, 4
m5C
CAGCTCGAGNNNN[NNNN]

TC

AceIII
CAGC
M.Sam53668II
CCATGG
1, 6
Unknown
CAGCTCCATGGNN[NNNN]

TC

AceIII
CAGC
M.Sen1781III
CCWWGG
1, 6
Unknown
CAGCTCCWWGGNN[NNNN]

TC

AceIII
CAGC
M.SmoLVI, M.Spn23FII, M.Spn556I,
TCTAGA
1, 6
m6A
CAGCTCTAGANNN[NNNN]

TC
M.Spn7465I, M.SpnRI, M.XbaI

AceIII
CAGC
M.SonIII
GCANNNN
3, 9
m6A
GCAGCTCGTCNNNN[NNNN]

TC

GTC

AceIII
CAGC
M.TdeII
CTCTTC
1, 2
m4C,
CAGCTCTTCNNNN[NNNN],

TC

m6A
CAGCTCTCTTCNN[NNNN]

AceIII
CAGC
M.TspRI
CASTG
1, 5
m5C
CAGCTCASTGNNN[NNNN]

TC

AceIII
CAGC
M1.AgoG2I, M2.AgoG2I
CCAGT
1, 4
m4C
CAGCTCCAGTNNN[NNNN]

TC

AceIII
CAGC
M1.BspACI
CCGC
1
m5C
CAGCTCCGCNNNN[NNNN]

TC

AceIII
CAGC
M1.HphI
TCACC
2
m5C
CAGCTCACCNNNN[NNNN]

TC

AceIII
CAGC
M1.Hpy32II
TCYYC
1
m6A
CAGCTCYYCNNNN[NNNN]

TC

AceIII
CAGC
M1.HpyAII, M1.MboII, M1.NcuI
TCTTC
1
m6A
CAGCTCTTCNNNN[NNNN]

TC

AceIII
CAGC
M1.LplAG30I
CCTTC
1
m5C
CAGCTCCTTCNNN[NNNN]

TC

AceIII
CAGC
M1.Sgr13350III, M2.Sgr13350III
GCTCTTC
2, 3
m4C,
CAGCTCTTCNNNN[NNNN]

TC

m6A

AceIII
CAGC
M2.ClaSBI, M1.Fli6794IV, M2.HphI,
TCACC
1
m6A
CAGCTCACCNNNN[NNNN]

TC
M2.Ngo3502II, M1.Ngo6140II, M1.NgoAXVI,

M1.NgoBVIII, M1.NgoFA19I, M2.NgoMVIII,

M2.PspHUN102I, M1.Ssp10II

AceIII
CAGC
M2.Hpy32II, M2.HpyAII, M2.MboII, M2.NcuI
TCTTC
2
m4C
CAGCTCTTCNNNN[NNNN]

TC

AceIII
CAGC
M2.HpyAVI, M1.MnlI
CCTC
1
m5C
CAGCTCCTCNNNN[NNNN]

TC

Bbr7I
GAAG
M.AatII, M.Lsp6406III
GACGTC
2, 5
m6A
GAAGACGTCNNNN[NNNN]

AC

Bbr7I
GAAG
M.AbaBs12I, M.Awo1030I, M.Cdu23823I,
CCWGG
1, 5
m5C
CCWGGAAGACNNNNNNN[NNNN],

AC
M.Csa8155II, M.Cun9529I, M.EasL1Dcm,

GAAGACCWGGNNN[NNNN]

M.Eba57Dcm, M.Ecl34399Dcm,

M.Eco12761Dcm, M.Eco3609Dcm,

M.Eco4255Dcm, M.EcoVR50Dcm,

M.EsaSP1II, M.Kpn34618Dcm,

M.Kpn38547Dcm, M.Kpn39I, M.Kpn43816I,

M.Kpn677ORFDcm, M.Kpn928Dcm,

M.KpnNIH1Dcm, M.KpnNIH10Dcm,

M.KpnNIH30Dcm, M.Kva120Dcm, M.Lsp48I,

M.Pmi1I, M.SpaD2III, M.SplRp8II,

M.SptADcm, M.Tsu2489III

Bbr7I
GAAG
M.AbrSp7I, M.Apa101655II, M.Ath21654I,
GANTC
2, 4
m6A
GAAGACTCNNNNN[NNNN]

AC
M.BdiNK6I, M.Bel76I, M.Bsp460I,

M.BspJKG1II, M.BspLA1II, M.BspRAC04II,

M.CagII, M.CcrMI, M.CcrNAI, M.CnaPQ2I,

M.Csp16704III, M.CspNS6I, M.CviBI,

M.CviQVI, M.Eli8509I, M.Fnu419I, M.Fsp71I,

M.HbaI, M.HhaII, M.HinfI, M.HinHia1I,

M.Hpy298IV, M.Hpy299IV, M.Hpy 32IV,

M.Hpy57IV, M.Hpy66IV, M.Hpy99IX,

M.HpyAIV, M.HpyFIV, M.HpyUM032IV,

M.HpyUM037IV, M.Mbo45III, M.Msp10I,

M.Msp73BI, M.MspAL11I, M.MspLW5II,

M.NhaXII, M.NpeUS61I, M.OspHL35I,

M.Pin17395II, M.Pla5III, M.PspLM6I,

M.RbaNRL2I, M.Rgi145II, M.Rmo1926I,

M.RpaIII, M.RsaII, M.RshIII, M.Rsp8I,

M.SmeC3I, M.SmoLV, M.Sna24252I,

M.SscL1I, M.SspM41II, M.SstE37II, M.ThaIII,

M.Xsp91I, M.ZmoCP4I

Bbr7I
GAAG
M.Acc65VI, M.SenAZIII
CTKMAG
2, 5
m6A
CTGAAGACNNNNNNN[NNNN]

AC

Bbr7I
GAAG
M.AciI
CCGC
1, 3
m5C
GCGGAAGACNNNNNNN[NNNN],

AC

GAAGACCGCNNNN[NNNN]

Bbr7I
GAAG
M.AflIII
ACRYGT
2, 5
m4C
GAAGACRYGTNNN[NNNN]

AC

Bbr7I
GAAG
M.AgsI
TTSAA
1, 5
m6A
TTGAAGACNNNNNNN[NNNN]

AC

Bbr7I
GAAG
M.Ama55I, M.CnaPQ2III, M.Rmo1926II,
CGGCCG
1, 6
m5C
CGGCCGAAGACNNNNNNN[NNNN],

AC
M.SenC1765IV, M.SspG1I, M.XorPXII

GAAGACGGCCGNN[NNNN]

Bbr7I
GAAG
M.AquI
CYCGRG
1, 6
m5C
CYCGRGAAGACNNNNNNN[NNNN],

AC

GAAGACYCGRGNN[NNNN]

Bbr7I
GAAG
M.AvaIX, M.Cfr10I, M.Nme18VI, M.VchAI,
RCCGGY
2, 5
m5C
GAAGACCGGYNNN[NNNN]

AC
M.Vch0395I

Bbr7I
GAAG
M.BcoSlacII, M.MspLW5III, M.Nbr128I,
CTCGAG
1, 6
m4C
CTCGAGAAGACNNNNNNN[NNNN],

AC
M.Psy18884II, M.Tam77409II, M.TbrGE1II,

GAAGACTCGAGNN[NNNN]

M.TpaRLII

Bbr7I
GAAG
M.BepI, M.FpsJV, M.Gel16401III, M.Pdi8503I
CGCG
1, 4
m5C
CGCGAAGACNNNNNNN[NNNN],

AC

GAAGACGCGNNNN[NNNN]

Bbr7I
GAAG
M.BsoBI
CYCGRG
1, 6
m4C
CYCGRGAAGACNNNNNNN[NNNN],

AC

GAAGACYCGRGNN[NNNN]

Bbr7I
GAAG
M.BsuFI, M.CpeAIV, M.MspI
CCGG
1, 4
m5C
CCGGAAGACNNNNNNN[NNNN],

AC

GAAGACCGGNNNN[NNNN]

Bbr7I
GAAG
M.Cab13497I, M.Dac11109III, M.Dco13527I,
CTAG
1, 4
m4C
CTAGAAGACNNNNNNN[NNNN],

AC
M.Djo10085I, M.Hbo11551I, M.Hka19301III,

GAAGACTAGNNNN[NNNN]

M.Hme33500I, M.HsaR1I, M.Hsi33800I,

M.HspKM1WII, M.HspNII, M.HtuI, M.HvoDSI,

M.Lba2029II, M.Mbo45II, M.MjaI, M.MthZI,

M.Nas12278I, M.Rfl3010I, M.SsmMI

Bbr7I
GAAG
M.CagV, M.Lsp6406I, M.Rsp7740I,
TTCGAA
1, 6
m6A
TTCGAAGACNNNNNNN[NNNN]

AC
M.RspPBTS2II, M.VbaLP2AI

Bbr7I
GAAG
M.CagVI
TCCGGA
1, 6
m6A
TCCGGAAGACNNNNNNN[NNNN]

AC

Bbr7I
GAAG
M.CagVIII
ACGCGT
2, 5
m4C
GAAGACGCGTNNN[NNNN]

AC

Bbr7I
GAAG
M.CfrBI, M.Sen1735IV, M.SenAnaIV
CCWWGG
1, 6
m4C
CCWWGGAAGACNNNNNNN[NNNN],

AC

GAAGACCWWGGNN[NNNN]

Bbr7I
GAAG
M.Cmu51329I, M.EcoJA03PDcm,
CCWGG
1, 5
Unknown
CCWGGAAGACNNNNNNN[NNNN],

AC
M.RorR1Dcm, M.Sen8391Dcm, M.ZalSM2II

GAAGACCWGGNNN[NNNN]

Bbr7I
GAAG
M.CviQIII, M.CviSIII, M.Hpy299XI,
TCGA
1, 4
m6A
TCGAAGACNNNNNNN[NNNN]

AC
M.Hpy30II, M.Hpy32X, M.Hpy99VII,

M.HpyAX, M.HpyFII, M.HpyUM032XVII,

M.Tam77409III, M.TaqI, M.TpaRLI,

M.TthHB8I

Bbr7I
GAAG
M.DdeI
CTNAG
1, 5
m5C
CTNAGAAGACNNNNNNN[NNNN],

AC

GAAGACTNAGNNN[NNNN]

Bbr7I
GAAG
M.DfeI, M.Fnu419II, M.Pen113II
CTNNAG
2, 5
m6A
CTGAAGACNNNNNNN[NNNN]

AC

Bbr7I
GAAG
M.FatI
CATG
1, 4
m5C
CATGAAGACNNNNNNN[NNNN],

AC

GAAGACATGNNNN[NNNN]

Bbr7I
GAAG
M.FisAW1I, M.ThaI, M.Tph12270II
CGCG
1, 4
m4C
CGCGAAGACNNNNNNN[NNNN],

AC

GAAGACGCGNNNN[NNNN]

Bbr71
GAAG
M.FnoB1II, M.Hal24586I, M.Hpy298V,
CCGG
1, 4
m4C
CCGGAAGACNNNNNNN[NNNN],

AC
M.Hpy299IX, M.Hpy30V, M.Hpy32XI,

GAAGACCGGNNNN[NNNN]

M.Hpy57V, M.Hpy66IX, M.Hpy99VIII,

M.HpyFV, M.HpyUM032IX, M.HpyUM037IX,

M.MjaVI, M.PspJDRI, M.TmeBII

Bbr7I
GAAG
M.FpsJIII
CTGAAG
5
m6A
CTGAAGACNNNNNNN[NNNN]

AC

Bbr7I
GAAG
M.GsuRed1IV, M.Osp807II, M.Tth111I
GACNNNG
2, 8
m6A
GAAGACNNNGTCN[NNNN]

AC

TC

Bbr7I
GAAG
M.Hka19301IV
TCGCGA
2, 5
m4C
TCGCGAAGACNNNNNNN[NNNN]

AC

Bbr7I
GAAG
M.Hpy188I, M.Hpy298IX, M.Hpy299V,
TCNGA
1, 5
m6A
TCNGAAGACNNNNNNN[NNNN]

AC
M.Hpy66V, M.HpyUM032V

Bbr7I
GAAG
M.Hpy298II, M.Hpy299II, M.Hpy66II,
ACNGT
2, 4
m4C
GAAGACNGTNNNN[NNNN]

AC
M.HpyUM032II, M.HpyUM037II

Bbr7I
GAAG
M.Hpy30VIII, M.Hpy32VIII, M.Hpy57III,
TCNNGA
1, 6
m6A
TCNNGAAGACNNNNNNN[NNNN]

AC
M.Hpy66VI, M.Hpy99XVIII, M.HpyFVIII,

M.HpyPVIII

Bbr7I
GAAG
M.Hpy30X, M.Hpy57VIII, M.HpyFXI, M.MhuI
CTNAG
1, 5
m4C
CTNAGAAGACNNNNNNN[NNNN],

AC

GAAGACTNAGNNN[NNNN]

Bbr7I
GAAG
M.Hpy99IV, M.HpyFORFX
CCNNGG
1, 6
m4C
CCNNGGAAGACNNNNNNN[NNNN],

AC

GAAGACCNNGGNN[NNNN]

Bbr7I
GAAG
M.Hpy99XI
ACGT
2, 3
m5C
GAAGACGTNNNNN[NNNN]

AC

Bbr7I
GAAG
M.HpyD27I
GAGG
2, 4
m6A,
GAAGACCTCNNNN[NNNN],

AC

m5C
GAGGAAGACNNNNNNN[NNNN]

Bbr7I
GAAG
M.Kpn555II, M.Mha183III
CTGAAG
2, 5
m6A
CTGAAGACNNNNNNN[NNNN]

AC

Bbr7I
GAAG
M.Lph145I, M.Lph93I
GGWGA
5
m6A
GGWGAAGACNNNNNNN[NNNN]

AC

Bbr7I
GAAG
M.MlyI, M.Pen113I
GASTC
2, 4
m6A
GAAGACTCNNNNN[NNNN]

AC

Bbr7I
GAAG
M.Msp1II
CCAGG
5
m4C
GAAGACCTGGNNN[NNNN],

AC

CCAGGAAGACNNNNNNN[NNNN]

Bbr7I
GAAG
M.Msp42II
CCWGG
1, 5
m4C
CCWGGAAGACNNNNNNN[NNNN],

AC

GAAGACCWGGNNN[NNNN]

Bbr7I
GAAG
M.Nme18III
CCAGA
2, 4
m5C
CCAGAAGACNNNNNNN[NNNN]

AC

Bbr7I
GAAG
M.NmeDI
VCCGGY
2, 5
m5C
GAAGACCGGBNNN[NNNN]

AC

Bbr7I
GAAG
M.NmeMC58III
CCAGA
1
m5C
TCTGGAAGACNNNNNNN[NNNN],

AC

GAAGACCAGANNN[NNNN]

Bbr7I
GAAG
M.Nsp209III, M.Nsp51109II
CTCGAG
1, 6
m5C
CTCGAGAAGACNNNNNNN[NNNN],

AC

GAAGACTCGAGNN[NNNN]

Bbr7I
GAAG
M.NspI, M.NspHI
RCATGY
2, 5
m5C
GAAGACATGYNNN[NNNN]

AC

Bbr7I
GAAG
M.Pam7686I
CCATGG
1, 6
m5C
CCATGGAAGACNNNNNNN[NNNN],

AC

GAAGACCATGGNN[NNNN]

Bbr7I
GAAG
M.Pfa23572II
CGATCG
1, 6
m5C
CGATCGAAGACNNNNNNN[NNNN],

AC

GAAGACGATCGNN[NNNN]

Bbr7I
GAAG
M.Pla5I
CTAG
1, 4
Unknown
CTAGAAGACNNNNNNN[NNNN],

AC

GAAGACTAGNNNN[NNNN]

Bbr7I
GAAG
M.Sam53668II
CCATGG
1, 6
Unknown
CCATGGAAGACNNNNNNN[NNNN],

AC

GAAGACCATGGNN[NNNN]

Bbr7I
GAAG
M.Sen1781III
CCWWGG
1, 6
Unknown
CCWWGGAAGACNNNNNNN[NNNN],

AC

GAAGACCWWGGNN[NNNN]

Bbr7I
GAAG
M.SmoLVI, M.Spn23FII, M.Spn556I,
TCTAGA
1, 6
m6A
TCTAGAAGACNNNNNNN[NNNN]

AC
M.Spn7465I, M.SpnRI, M.XbaI

Bbr7I
GAAG
M.SonIII
GACNNNN
2, 8
m6A
GAAGACNNNNTGC[NNNN]

AC

TGC

Bbr7I
GAAG
M.TdeII
CTCTTC
1, 2
m4C,
GAAGAGAAGACNNNNNNN[NNNN],

AC

m6A
GAAGACTCTTCNN[NNNN]

Bbr7I
GAAG
M.TspRI
CASTG
1, 5
m5C
CASTGAAGACNNNNNNN[NNNN],

AC

GAAGACASTGNNN[NNNN]

Bbr7I
GAAG
M.XmnI
GAANNNN
2, 9
m6A
GAAGACNTTCNNN[NNNN]

AC

TTC

Bbr7I
GAAG
M1.AgoG2I, M2.AgoG2I
CCAGT
1, 4
m4C
ACTGGAAGACNNNNNNN[NNNN],

AC

GAAGACTGGNNNN[NNNN],

GAAGACCAGTNNN[NNNN]

Bbr7I
GAAG
M1.BceSIII
ACGGC
2
m4C
GAAGACGGCNNNN[NNNN]

AC

Bbr7I
GAAG
M1.BspACI
CCGC
1
m5C
GCGGAAGACNNNNNNN[NNNN],

AC

GAAGACCGCNNNN[NNNN]

Bbr7I
GAAG
M1.Dsh12II, M2.Dsh12II
GACGGC
3, 4
m4C
GAAGACGGCNNNN[NNNN]

AC

Bbr7I
GAAG
M1.HphI
GGTGA
4
m5C
GGTGAAGACNNNNNNN[NNNN]

AC

Bbr7I
GAAG
M1.LplAG30I
GAAGG
5
m5C
GAAGACCTTCNNN[NNNN],

AC

GAAGGAAGACNNNNNNN[NNNN]

Bbr7I
GAAG
M2.BceSIII, M1.Pru9I, M2.Pru9I
ACGGC
2, 4
m4C
GAAGACGGCNNNN[NNNN]

AC

Bbr7I
GAAG
M2.BfiI
ACTGGG
2
m4C
GAAGACTGGGNNN[NNNN]

AC

Bbr7I
GAAG
M2.BfuAI
ACCTGC
2
m5C
GAAGACCTGCNNN[NNNN]

AC

Bbr71
GAAG
M2.ClaSBI, M1.Fli6794IV, M2.HphI,
GGTGA
5
m6A
GGTGAAGACNNNNNNN[NNNN]

AC
M2.Ngo3502II, M1.Ngo6140II, M1.NgoAXVI,

M1.NgoBVIII, M1.NgoFA19I, M2.NgoMVIII,

M2.PspHUN102I, M1.Ssp10II

Bbr7I
GAAG
M2.HgaI, M1.LlaJI
GACGC
3
m5C
GAAGACGCNNNNN[NNNN]

AC

Bbr7I
GAAG
M2.HpyAVI, M1.MnlI
GAGG
4
m5C
GAAGACCTCNNNN[NNNN],

AC

GAGGAAGACNNNNNNN[NNNN]

Bbr7I
GAAG
M2.Nme579IV, M2.NmeMC58II
GACGC
2
m6A
GAAGACGCNNNNN[NNNN]

AC

BbsI, BbvII,
GAAG
M.AbaBs12I, M.Awo1030I, M.Cdu23823I,
CCWGG
1, 5
m5C
CCWGGAAGACNN[NNNN]

Bbv16II, BpiI,
AC
M.Csa8155II, M.Cun9529I, M.EasL1Dcm,

BpuAI, Bsc91I,

M.Eba57Dcm, M.Ecl34399Dcm,

BspBS31I,

M.Eco12761Dcm, M.Eco3609Dcm,

BspIS4I,

M.Eco4255Dcm, M.EcoVR50Dcm,

BspTS514I,

M.EsaSP1II, M.Kpn34618Dcm,

BstBS32I, BstTS5I,

M.Kpn38547Dcm, M.Kpn39I, M.Kpn43816I,

BstV2I

M.Kpn677ORFDcm, M.Kpn928Dcm,

M.KpnNIH1Dcm, M.KpnNIH10Dcm,

M.KpnNIH30Dcm, M.Kva120Dcm, M.Lsp48I,

M.Pmi1I, M.SpaD2III, M.SplRp8II,

M.SptADcm, M.Tsu2489III

BbsI, BbvII,
GAAG
M.AbrSp7I, M.Apa101655II, M.Ath21654I,
GANTC
2, 4
m6A
GAAGACTC[NNNN]

Bbv16II, BpiI,
AC
M.BdiNK6I, M.Bel76I, M.Bsp460I,

BpuAI, Bsc91I,

M.BspJKG1II, M.BspLA1II, M.BspRAC04II,

BspBS31I,

M.CagII, M.CcrMI, M.CcrNAI, M.CnaPQ2I,

BspIS4I,

M.Csp16704III, M.CspNS6I, M.CviBI,

BspTS514I,

M.CviQVI, M.Eli8509I, M.Fnu419I, M.Fsp71I,

BstBS32I, BstTS5I,

M.HbaI, M.HhaII, M.HinfI, M.HinHia1I,

BstV2I

M.Hpy298IV, M.Hpy299IV, M.Hpy32IV,

M.Hpy57IV, M.Hpy66IV, M.Hpy99IX,

M.HpyAIV, M.HpyFIV, M.HpyUM032IV,

M.HpyUM037IV, M.Mbo45III, M.Msp10I,

M.Msp73BI, M.MspAL11I, M.MspLW5II,

M.NhaXII, M.NpeUS61I, M.OspHL35I,

M.Pin17395II, M.Pla5III, M.PspLM6I,

M.RbaNRL2I, M.Rgi145II, M.Rmo1926I,

M.RpaIII, M.RsaII, M.RshIII, M.Rsp8I,

M.SmeC3I, M.SmoLV, M.Sna24252I,

M.SscL1I, M.SspM41II, M.SstE37II, M.ThaIII,

M.Xsp91I, M.ZmoCP4I

BbsI, BbvII,
GAAG
M.Acc65VI, M.SenAZIII
CTKMAG
2, 5
m6A
CTGAAGACNN[NNNN]

Bbv16II, BpiI,
AC

BpuAI, Bsc91I,

BspBS31I,

BspIS4I,

BspTS514I,

BstBS32I, BstTS5I,

BstV2I

BbsI, BbvII,
GAAG
M.AciI
GCGG
2, 4
m5C
GCGGAAGACNN[NNNN]

Bbv16II, BpiI,
AC

BpuAI, Bsc91I,

BspBS31I,

BspIS4I,

BspTS514I,

BstBS32I, BstTS5I,

BstV2I

BbsI, BbvII,
GAAG
M.AgsI
TTSAA
1, 5
m6A
TTGAAGACNN[NNNN]

Bbv16II, BpiI,
AC

BpuAI, Bsc91I,

BspBS31I,

BspIS4I,

BspTS514I,

BstBS32I, BstTS5I,

BstV2I

BbsI, BbvII,
GAAG
M.Ama55I, M.CnaPQ2III, M.Rmo1926II,
CGGCCG
1, 6
m5C
CGGCCGAAGACNN[NNNN]

Bbv16II, BpiI,
AC
M.SenC1765IV, M.SspG1I, M.XorPXII

BpuAI, Bsc91I,

BspBS31I,

BspIS4I,

BspTS514I,

BstBS32I, BstTS5I,

BstV2I

BbsI, BbvII,
GAAG
M.AquI
CYCGRG
1, 6
m5C
CYCGRGAAGACNN[NNNN]

Bbv16II, BpiI,
AC

BpuAI, Bsc91I,

BspBS31I,

BspIS4I,

BspTS514I,

BstBS32I, BstTS5I,

BstV2I

BbsI, BbvII,
GAAG
M.BcoSlacII, M.MspLW5III, M.Nbr128I,
CTCGAG
1, 6
m4C
CTCGAGAAGACNN[NNNN]

Bbv16II, BpiI,
AC
M.Psy18884II, M.Tam77409II, M.TbrGE1II,

BpuAI, Bsc91I,

M.TpaRLII

BspBS31I,

BspIS4I,

BspTS514I,

BstBS32I, BstTS5I,

BstV2I

BbsI, BbvII,
GAAG
M.BepI, M.FpsJV, M.Gel16401III, M.Pdi8503I
CGCG
1, 4
m5C
CGCGAAGACNN[NNNN]

Bbv16II, BpiI,
AC

BpuAI, Bsc91I,

BspBS31I,

BspIS4I,

BspTS514I,

BstBS32I, BstTS5I,

BstV2I

BbsI, BbvII,
GAAG
M.BsoBI
CYCGRG
1, 6
m4C
CYCGRGAAGACNN[NNNN]

Bbv16II, BpiI,
AC

BpuAI, Bsc91I,

BspBS31I,

BspIS4I,

BspTS514I,

BstBS32I, BstTS5I,

BstV2I

BbsI, BbvII,
GAAG
M.BsuFI, M.CpeAIV, M.MspI
CCGG
1, 4
m5C
CCGGAAGACNN[NNNN]

Bbv16II, BpiI,
AC

BpuAI, Bsc91I,

BspBS31I,

BspIS4I,

BspTS514I,

BstBS32I, BstTS5I,

BstV2I

BbsI, BbvII,
GAAG
M.Cab13497I, M.Dac11109III, M.Dco13527I,
CTAG
1, 4
m4C
CTAGAAGACNN[NNNN]

Bbv16II, BpiI,
AC
M.Djo10085I, M.Hbo11551I, M.Hka19301III,

BpuAI, Bsc91I,

M.Hme33500I, M.HsaR1I, M.Hsi33800I,

BspBS31I,

M.HspKM1WII, M.HspNII, M.HtuI, M.HvoDSI,

BspIS4I,

M.Lba2029II, M.Mbo45II, M.MjaI, M.MthZI,

BspTS514I,

M.Nas12278I, M.Rfl3010I, M.SsmMI

BstBS32I, BstTS5I,

BstV2I

BbsI, BbvII,
GAAG
M.CagV, M.Lsp6406I, M.Rsp7740I,
TTCGAA
1, 6
m6A
TTCGAAGACNN[NNNN]

Bbv16II, BpiI,
AC
M.RspPBTS2II, M.VbaLP2AI

BpuAI, Bsc91I,

BspBS31I,

BspIS4I,

BspTS514I,

BstBS32I, BstTS5I,

BstV2I

BbsI, BbvII,
GAAG
M.CagVI
TCCGGA
1, 6
m6A
TCCGGAAGACNN[NNNN]

Bbv16II, BpiI,
AC

BpuAI, Bsc91I,

BspBS31I,

BspIS4I,

BspTS514I,

BstBS32I, BstTS5I,

BstV2I

BbsI, BbvII,
GAAG
M.CfrBI, M.Sen1735IV, M.SenAnaIV
CCWWGG
1, 6
m4C
CCWWGGAAGACNN[NNNN]

Bbv16II, BpiI,
AC

BpuAI, Bsc91I,

BspBS31I,

BspIS4I,

BspTS514I,

BstBS32I, BstTS5I,

BstV2I

BbsI, BbvII,
GAAG
M.Cmu51329I, M.EcoJA03PDcm,
CCWGG
1, 5
Unknown
CCWGGAAGACNN[NNNN]

Bbv16II, BpiI,
AC
M.RorR1Dcm, M.Sen8391Dcm, M.ZalSM2II

BpuAI, Bsc91I,

BspBS31I,

BspIS4I,

BspTS514I,

BstBS32I, BstTS5I,

BstV2I

BbsI, BbvII,
GAAG
M.CviQIII, M.CviSIII, M.Hpy299XI,
TCGA
1, 4
m6A
TCGAAGACNN[NNNN]

Bbv16II, BpiI,
AC
M.Hpy30II, M.Hpy32X, M.Hpy99VII,

BpuAI, Bsc91I,

M.HpyAX, M.HpyFII, M.HpyUM032XVII,

BspBS31I,

M.Tam77409III, M.TaqI, M.TpaRLI,

BspIS4I,

M.TthHB8I

BspTS514I,

BstBS32I, BstTS5I,

BstV2I

BbsI, BbvII,
GAAG
M.DdeI
CTNAG
1, 5
m5C
CTNAGAAGACNN[NNNN]

Bbv16II, BpiI,
AC

BpuAI, Bsc91I,

BspBS31I,

BspIS4I,

BspTS514I,

BstBS32I, BstTS5I,

BstV2I

BbsI, BbvII,
GAAG
M.DfeI, M.Fnu419II, M.Pen113II
CTNNAG
2, 5
m6A
CTGAAGACNN[NNNN]

Bbv16II, BpiI,
AC

BpuAI, Bsc91I,

BspBS31I,

BspIS4I,

BspTS514I,

BstBS32I, BstTS5I,

BstV2I

BbsI, BbvII,
GAAG
M.FatI
CATG
1, 4
m5C
CATGAAGACNN[NNNN]

Bbv16II, BpiI,
AC

BpuAI, Bsc91I,

BspBS31I,

BspIS4I,

BspTS514I,

BstBS32I, BstTS5I,

BstV2I

BbsI, BbvII,
GAAG
M.FisAW1I, M.ThaI, M.Tph12270II
CGCG
1, 4
m4C
CGCGAAGACNN[NNNN]

Bbv16II, BpiI,
AC

BpuAI, Bsc91I,

BspBS31I,

BspIS4I,

BspTS514I,

BstBS32I, BstTS5I,

BstV2I

BbsI, BbvII,
GAAG
M.FnoB1II, M.Hal24586I, M.Hpy298V,
CCGG
1, 4
m4C
CCGGAAGACNN[NNNN]

Bbv16II, BpiI,
AC
M.Hpy299IX, M.Hpy30V, M.Hpy32XI,

BpuAI, Bsc91I,

M.Hpy57V, M.Hpy66IX, M.Hpy99VIII,

BspBS31I,

M.HpyFV, M.HpyUM032IX, M.HpyUM037IX,

BspIS4I,

M.MjaVI, M.PspJDRI, M.TmeBII

BspTS514I,

BstBS32I, BstTS5I,

BstV2I

BbsI, BbvII,
GAAG
M.FpsJIII
CTGAAG
5
m6A
CTGAAGACNN[NNNN]

Bbv16II, BpiI,
AC

BpuAI, Bsc91I,

BspBS31I,

BspIS4I,

BspTS514I,

BstBS32I, BstTS5I,

BstV2I

BbsI, BbvII,
GAAG
M.Hka19301IV
TCGCGA
2, 5
m4C
TCGCGAAGACNN[NNNN]

Bbv16II, BpiI,
AC

BpuAI, Bsc91I,

BspBS31I,

BspIS4I,

BspTS514I,

BstBS32I, BstTS5I,

BstV2I

BbsI, BbvII,
GAAG
M.Hpy188I, M.Hpy298IX, M.Hpy299V,
TCNGA
1, 5
m6A
TCNGAAGACNN[NNNN]

Bbv16II, BpiI,
AC
M.Hpy66V, M.HpyUM032V

BpuAI, Bsc91I,

BspBS31I,

BspIS4I,

BspTS514I,

BstBS32I, BstTS5I,

BstV2I

BbsI, BbvII,
GAAG
M.Hpy30VIII, M.Hpy32VIII, M.Hpy57III,
TCNNGA
1, 6
m6A
TCNNGAAGACNN[NNNN]

Bbv16II, BpiI,
AC
M.Hpy66VI, M.Hpy99XVIII, M.HpyFVIII,

BpuAI, Bsc91I,

M.HpyPVIII

BspBS31I,

BspIS4I,

BspTS514I,

BstBS32I, BstTS5I,

BstV21

BbsI, BbvII,
GAAG
M.Hpy30X, M.Hpy57VIII, M.HpyFXI, M.MhuI
CTNAG
1, 5
m4C
CTNAGAAGACNN[NNNN]

Bbv16II, BpiI,
AC

BpuAI, Bsc91I,

BspBS31I,

BspIS4I,

BspTS514I,

BstBS32I, BstTS5I,

BstV2I

BbsI, BbvII,
GAAG
M.Hpy99IV, M.HpyFORFX
CCNNGG
1, 6
m4C
CCNNGGAAGACNN[NNNN]

Bbv16II, BpiI,
AC

BpuAI, Bsc91I,

BspBS31I,

BspIS4I,

BspTS514I,

BstBS32I, BstTS5I,

BstV2I

BbsI, BbvII,
GAAG
M.Hpy99XI
ACGT
2, 3
m5C
GAAGACGT[NNNN]

Bbv16II, BpiI,
AC

BpuAI, Bsc91I,

BspBS31I,

BspIS4I,

BspTS514I,

BstBS32I, BstTS5I,

BstV2I

BbsI, BbvII,
GAAG
M.HpyD27I
GAGG
2, 4
m6A,
GAGGAAGACNN[NNNN]

Bbv16II, BpiI,
AC

m5C

BpuAI, Bsc91I,

BspBS31I,

BspIS4I,

BspTS514I,

BstBS32I, BstTS5I,

BstV2I

BbsI, BbvII,
GAAG
M.Kpn555II, M.Mha183III
CTGAAG
2, 5
m6A
CTGAAGACNN[NNNN]

Bbv16II, BpiI,
AC

BpuAI, Bsc91I,

BspBS31I,

BspIS4I,

BspTS514I,

BstBS32I, BstTS5I,

BstV2I

BbsI, BbvII,
GAAG
M.Lph145I, M.Lph93I
GGWGA
5
m6A
GGWGAAGACNN[NNNN]

Bbv16II, BpiI,
AC

BpuAI, Bsc91I,

BspBS31I,

BspIS4I,

BspTS514I,

BstBS32I, BstTS5I,

BstV2I

BbsI, BbvII,
GAAG
M.MlyI, M.Pen113I
GASTC
2, 4
m6A
GAAGACTC[NNNN]

Bbv16II, BpiI,
AC

BpuAI, Bsc91I,

BspBS31I,

BspIS4I,

BspTS514I,

BstBS32I, BstTS5I,

BstV2I

BbsI, BbvII,
GAAG
M.Msp1II
CCAGG
5
m4C
CCAGGAAGACNN[NNNN]

Bbv16II, BpiI,
AC

BpuAI, Bsc91I,

BspBS31I,

BspIS4I,

BspTS514I,

BstBS32I, BstTS5I,

BstV2I

BbsI, BbvII,
GAAG
M.Msp42II
CCWGG
1, 5
m4C
CCWGGAAGACNN[NNNN]

Bbv16II, BpiI,
AC

BpuAI, Bsc91I,

BspBS31I,

BspIS4I,

BspTS514I,

BstBS32I, BstTS5I,

BstV2I

BbsI, BbvII,
GAAG
M.Nme18III
CCAGA
2, 4
m5C
CCAGAAGACNN[NNNN]

Bbv16II, BpiI,
AC

BpuAI, Bsc91I,

BspBS31I,

BspIS4I,

BspTS514I,

BstBS32I, BstTS5I,

BstV2I

BbsI, BbvII,
GAAG
M.NmeMC58III
TCTGG
5
m5C
TCTGGAAGACNN[NNNN]

Bbv16II, BpiI,
AC

BpuAI, Bsc91I,

BspBS31I,

BspIS4I,

BspTS514I,

BstBS32I, BstTS5I,

BstV2I

BbsI, BbvII,
GAAG
M.Nsp209III, M.Nsp51109II
CTCGAG
1, 6
m5C
CTCGAGAAGACNN[NNNN]

Bbv16II, BpiI,
AC

BpuAI, Bsc91I,

BspBS31I,

BspIS4I,

BspTS514I,

BstBS32I, BstTS5I,

BstV2I

BbsI, BbvII,
GAAG
M.Pam7686I
CCATGG
1, 6
m5C
CCATGGAAGACNN[NNNN]

Bbv16II, BpiI,
AC

BpuAI, Bsc91I,

BspBS31I,

BspIS4I,

BspTS514I,

BstBS32I, BstTS5I,

BstV2I

BbsI, BbvII,
GAAG
M.Pfa23572II
CGATCG
1, 6
m5C
CGATCGAAGACNN[NNNN]

Bbv16II, BpiI,
AC

BpuAI, Bsc91I,

BspBS31I,

BspIS4I,

BspTS514I,

BstBS32I, BstTS5I,

BstV2I

BbsI, BbvII,
GAAG
M.Pla5I
CTAG
1, 4
Unknown
CTAGAAGACNN[NNNN]

Bbv16II, BpiI,
AC

BpuAI, Bsc91I,

BspBS31I,

BspIS4I,

BspTS514I,

BstBS32I, BstTS5I,

BstV2I

BbsI, BbvII,
GAAG
M.Sam53668II
CCATGG
1, 6
Unknown
CCATGGAAGACNN[NNNN]

Bbv16II, BpiI,
AC

BpuAI, Bsc91I,

BspBS31I,

BspIS4I,

BspTS514I,

BstBS32I, BstTS5I,

BstV2I

BbsI, BbvII,
GAAG
M.Sen1781III
CCWWGG
1, 6
Unknown
CCWWGGAAGACNN[NNNN]

Bbv16II, BpiI,
AC

BpuAI, Bsc91I,

BspBS31I,

BspIS4I,

BspTS514I,

BstBS32I, BstTS5I,

BstV2I

BbsI, BbvII,
GAAG
M.SmoLVI, M.Spn23FII, M.Spn556I,
TCTAGA
1, 6
m6A
TCTAGAAGACNN[NNNN]

Bbv16II, BpiI,
AC
M.Spn7465I, M.SpnRI, M.XbaI

BpuAI, Bsc91I,

BspBS31I,

BspIS4I,

BspTS514I,

BstBS32I, BstTS5I,

BstV2I

BbsI, BbvII,
GAAG
M.TdeII
GAAGAG
5, 6
m6A,
GAAGAGAAGACNN[NNNN]

Bbv16II, BpiI,
AC

m4C

BpuAI, Bsc91I,

BspBS31I,

BspIS4I,

BspTS514I,

BstBS32I, BstTS5I,

BstV2I

BbsI, BbvII,
GAAG
M.TspRI
CASTG
1, 5
m5C
CASTGAAGACNN[NNNN]

Bbv16II, BpiI,
AC

BpuAI, Bsc91I,

BspBS31I,

BspIS4I,

BspTS514I,

BstBS32I, BstTS5I,

BstV21

BbsI, BbvII,
GAAG
M1.AgoG2I, M2.AgoG2I
ACTGG
2, 5
m4C
ACTGGAAGACNN[NNNN]

Bbv16II, BpiI,
AC

BpuAI, Bsc91I,

BspBS31I,

BspIS4I,

BspTS514I,

BstBS32I, BstTS5I,

BstV2I

BbsI, BbvII,
GAAG
M1.BspACI
GCGG
4
m5C
GCGGAAGACNN[NNNN]

Bbv16II, BpiI,
AC

BpuAI, Bsc91I,

BspBS31I,

BspIS4I,

BspTS514I,

BstBS32I, BstTS5I,

BstV2I

BbsI, BbvII,
GAAG
M1.HphI
GGTGA
4
m5C
GGTGAAGACNN[NNNN]

Bbv16II, BpiI,
AC

BpuAI, Bsc91I,

BspBS31I,

BspIS4I,

BspTS514I,

BstBS32I, BstTS5I,

BstV2I

BbsI, BbvII,
GAAG
M1.LplAG30I
GAAGG
5
m5C
GAAGGAAGACNN[NNNN]

Bbv16II, BpiI,
AC

BpuAI, Bsc91I,

BspBS31I,

BspIS4I,

BspTS514I,

BstBS32I, BstTS5I,

BstV2I

BbsI, BbvII,
GAAG
M2.ClaSBI, M1.Fli6794IV, M2.HphI,
GGTGA
5
m6A
GGTGAAGACNN[NNNN]

Bbv16II, BpiI,
AC
M2.Ngo3502II, M1.Ngo6140II, M1.NgoAXVI,

BpuAI, Bsc91I,

M1.NgoBVIII, M1.NgoFA19I, M2.NgoMVIII,

BspBS31I,

M2.PspHUN102I, M1.Ssp10II

BspIS4I,

BspTS514I,

BstBS32I, BstTS5I,

BstV2I

BbsI, BbvII,
GAAG
M2.HgaI, M1.LlaJI
GACGC
3
m5C
GAAGACGC[NNNN]

Bbv16II, BpiI,
AC

BpuAI, Bsc91I,

BspBS31I,

BspIS4I,

BspTS514I,

BstBS32I, BstTS5I,

BstV2I

BbsI, BbvII,
GAAG
M2.HpyAVI, M1.MnlI
GAGG
4
m5C
GAGGAAGACNN[NNNN]

Bbv16II, BpiI,
AC

BpuAI, Bsc91I,

BspBS31I,

BspIS4I,

BspTS514I,

BstBS32I, BstTS5I,

BstV21

BbsI, BbvII,
GAAG
M2.Nme579IV, M2.NmeMC58II
GACGC
2
m6A
GAAGACGC[NNNN]

Bbv16II, BpiI,
AC

BpuAI, Bsc91I,

BspBS31I,

BspIS4I,

BspTS514I,

BstBS32I, BstTS5I,

BstV2I

Bco5I, Bco116I,
CTCTT
M.AbrSp7I, M.Apa101655II, M.Ath21654I,
GANTC
2, 4
m6A
GACTCTTCN[NNN]

BcoKI, BseZI,
C
M.BdiNK6I, M.Bel76I, M.Bsp460I,

Bst6I, Bsu6I,

M.BspJKG1II, M.BspLA1II, M.BspRAC04II,

CatHI, Eam1104I,

M.CagII, M.CcrMI, M.CcrNAI, M.CnaPQ2I,

EarI, Ksp632I

M.Csp16704III, M.CspNS6I, M.CviBI,

M.CviQVI, M.Eli8509I, M.Fnu419I, M.Fsp71I,

M.HbaI, M.HhaII, M.HinfI, M.HinHia1I,

M.Hpy298IV, M.Hpy299IV, M.Hpy32IV,

M.Hpy57IV, M.Hpy66IV, M.Hpy99IX,

M.HpyAIV, M.HpyFIV, M.HpyUM032IV,

M.HpyUM037IV, M.Mbo45III, M.Msp10I,

M.Msp73BI, M.MspAL11I, M.MspLW5II,

M.NhaXII, M.NpeUS61I, M.OspHL35I,

M.Pin17395II, M.Pla5III, M.PspLM6I,

M.RbaNRL2I, M.Rgi145II, M.Rmo1926I,

M.RpaIII, M.RsaII, M.RshIII, M.Rsp8I,

M.SmeC3I, M.SmoLV, M.Sna24252I,

M.SscL1I, M.SspM41II, M.SstE37II, M.ThaIII,

M.Xsp91I, M.ZmoCP4I

Bco5I, Bco116I,
CTCTT
M.AluBI
AGCT
1, 4
m6A
AGCTCTTCN[NNN]

BcoKI, BseZI,
C

Bst6I, Bsu6I,

CatHI, Eam1104I,

EarI, Ksp632I

Bco5I, Bco116I,
CTCTT
M.AluI, M.CspCY2I
AGCT
2, 3
m5C
AGCTCTTCN[NNN]

BcoKI, BseZI,
C

Bst6I, Bsu6I,

CatHI, Eam1104I,

EarI, Ksp632I

Bco5I, Bco116I,
CTCTT
M.Alw26I
GTCTC
3, 2
m5C,
GTCTCTTCN[NNN]

BcoKI, BseZI,
C

m6A

Bst6I, Bsu6I,

CatHI, Eam1104I,

EarI, Ksp632I

Bco5I, Bco116I,
CTCTT
M.AspDUT2II, M.BstYI, M.CagIII,
RGATCY
2, 5
m4C

BcoKI, BseZI,
C
M.Mpa1757V, M.Mru1279V, M.RsaIII,

Bst6I, Bsu6I,

M.XhoII

CatHI, Eam1104I,

EarI, Ksp632I

Bco5I, Bco116I,
CTCTT
M.AspJHL3I, M2.Psp320WII
GAGCTC
3, 4
m4C
GAGCTCTTCN[NNN]

BcoKI, BseZI,
C

Bst6I, Bsu6I,

CatHI, Eam1104I,

EarI, Ksp632I

Bco5I, Bco116I,
CTCTT
M.AvaV, M.SliSI
GATC
1, 4
m4C
GATCTCTTCN[NNN]

BcoKI, BseZI,
C

Bst6I, Bsu6I,

CatHI, Eam1104I,

EarI, Ksp632I

Bco5I, Bco116I,
CTCTT
M.BanII, M.EcoT38I
GRGCYC
3, 4
m5C
GRGCTCTTCN[NNN]

BcoKI, BseZI,
C

Bst6I, Bsu6I,

CatHI, Eam1104I,

EarI, Ksp632I

Bco5I, Bco116I,
CTCTT
M.BceSII
GGWCC
1, 5
m5C
GGWCCTCTTCN[NNN]

BcoKI, BseZI,
C

Bst6I, Bsu6I,

CatHI, Eam1104I,

EarI, Ksp632I

Bco5I, Bco116I,
CTCTT
M.BglII
AGATCT
2, 5
m4C
AGATCTCTTCN [NNN]

BcoKI, BseZI,
C

Bst6I, Bsu6I,

CatHI, Eam1104I,

EarI, Ksp632I

Bco5I, Bco116I,
CTCTT
M.Ble402III, M.Psp10HI
SAGCTS
3, 4
m4C
SAGCTCTTCN[NNN]

BcoKI, BseZI,
C

Bst6I, Bsu6I,

CatHI, Eam1104I,

EarI, Ksp632I

Bco5I, Bco116I,
CTCTT
M.Bsp14III, M.Bsp3003I, M.Bsp3004I,
GATC
1, 4
m5C
GATCTCTTCN[NNN]

BcoKI, BseZI,
C
M.CalB3III, M.CpeAIII, M.Esu35585I,

Bst6I, Bsu6I,

M.FpsJIV, M.Lba2031I, M.Lbo2004I,

CatHI, Eam1104I,

M.LlaKR2I, M.Lmo1042I, M.Lmo7956II,

EarI, Ksp632I

M.Lsp10393II, M.MspI7I, M.Sau3AI, M.Sba3I,

M.Sin395I, M.Spn23FI, M.Sth368I

Bco5I, Bco116I,
CTCTT
M.CocIII, M.Fsi4947II, M.TdeIII,
GGNCC
1, 5
m4C
GGNCCTCTTCN[NNN]

BcoKI, BseZI,
C
M.Tph12270I

Bst6I, Bsu6I,

CatHI, Eam1104I,

EarI, Ksp632I

Bco5I, Bco116I,
CTCTT
M.CviJI
RGCB
2, 3
m5C
VGCTCTTCN[NNN]

BcoKI, BseZI,
C

Bst6I, Bsu6I,

CatHI, Eam1104I,

EarI, Ksp632I

Bco5I, Bco116I,
CTCTT
M.Dac11109II, M.Hfo6591I, M.MhuII,
GTAC
1, 4
m4C
GTACTCTTCN[NNN]

BcoKI, BseZI,
C
M.MjaV, M.MlaZII, M.MspLW5I, M.RsaI

Bst6I, Bsu6I,

CatHI, Eam1104I,

EarI, Ksp632I

Bco5I, Bco116I,
CTCTT
M.Esp3I
CGTCTC
4, 3
m5C,
CGTCTCTTCN[NNN]

BcoKI, BseZI,
C

m6A

Bst6I, Bsu6I,

CatHI, Eam1104I,

EarI, Ksp632I

Bco5I, Bco116I,
CTCTT
M.HpyD27I
CCTC
1, 3
m5C,
CCTCTTCN[NNN]

BcoKI, BseZI,
C

m6A

Bst6I, Bsu6I,

CatHI, Eam1104I,

EarI, Ksp6321

Bco5I, Bco116I,
CTCTT
M.MjaII
GGNCC
1, 5
m5C
GGNCCTCTTCN[NNN]

BcoKI, BseZI,
C

Bst6I, Bsu6I,

CatHI, Eam1104I,

EarI, Ksp632I

Bco5I, Bco116I,
CTCTT
M.MlyI, M.Pen113I
GASTC
2, 4
m6A
GACTCTTCN[NNN]

BcoKI, BseZI,
C

Bst6I, Bsu6I,

CatHI, Eam1104I,

EarI, Ksp632I

Bco5I, Bco116I,
CTCTT
M.Mma5219II, M.TarMY3I
AGCT
2, 3
m4C
AGCTCTTCN[NNN]

BcoKI, BseZI,
C

Bst6I, Bsu6I,

CatHI, Eam1104I,

EarI, Ksp632I

Bco5I, Bco116I,
CTCTT
M.MpeII
RGCY
2, 3
m5C
RGCTCTTCN[NNN]

BcoKI, BseZI,
C

Bst6I, Bsu6I,

CatHI, Eam1104I,

EarI, Ksp632I

Bco5I, Bco116I,
CTCTT
M.NheI
GCTAGC
1, 6
m4C
GCTAGCTCTTCN[NNN]

BcoKI, BseZI,
C

Bst6I, Bsu6I,

CatHI, Eam1104I,

EarI, Ksp632I

Bco5I, Bco116I,
CTCTT
M.Nme579III, M.NmeSI, M.ScaI, M.Ssp6714II
AGTACT
2, 5
m4C
AGTACTCTTCN[NNN]

BcoKI, BseZI,
C

Bst6I, Bsu6I,

CatHI, Eam1104I,

EarI, Ksp632I

Bco5I, Bco116I,
CTCTT
M.Pae10332II, M1.Pae50071I,
CAGCTC
3, 4
m4C
CAGCTCTTCN[NNN]

BcoKI, BseZI,
C
M2.Pae50071I

Bst6I, Bsu6I,

CatHI, Eam1104I,

EarI, Ksp632I

Bco5I, Bco116I,
CTCTT
M.SacI, M.Sgr13350I
GAGCTC
3, 4
m5C
GAGCTCTTCN[NNN]

BcoKI, BseZI,
C

Bst6I, Bsu6I,

CatHI, Eam1104I,

EarI, Ksp632I

Bco5I, Bco116I,
CTCTT
M.SuaII
RGATCY
2, 5
m5C
RGATCTCTTCN[NNN]

BcoKI, BseZI,
C

Bst6I, Bsu6I,

CatHI, Eam1104I,

EarI, Ksp632I

Bco5I, Bco116I,
CTCTT
M.TacIII
GGCCT
4
m4C
GGCCTCTTCN[NNN]

BcoKI, BseZI,
C

Bst6I, Bsu6I,

CatHI, Eam1104I,

EarI, Ksp632I

Bco5I, Bco116I,
CTCTT
M.Tam77409IV
CCRCTC
4
m4C
CCRCTCTTCN[NNN]

BcoKI, BseZI,
C

Bst6I, Bsu6I,

CatHI, Eam1104I,

EarI, Ksp632I

Bco5I, Bco116I,
CTCTT
M.TspPM5I
AGGCCT
2, 5
m4C
AGGCCTCTTCN[NNN]

BcoKI, BseZI,
C

Bst6I, Bsu6I,

CatHI, Eam1104I,

EarI, Ksp6321

Bco5I, Bco116I,
CTCTT
M.XmnI
GAANNNN
2, 9
m6A
GAANCTCTTCN[NNN]

BcoKI, BseZI,
C

TTC

Bst6I, Bsu6I,

CatHI, Eam1104I,

EarI, Ksp632I

Bco5I, Bco116I,
CTCTT
M1.Sgr13350III, M2.Sgr13350III
GCTCTTC
2, 3
m4C,
GCTCTTCN[NNN]

BcoKI, BseZI,
C

m6A

Bst6I, Bsu6I,

CatHI, Eam1104I,

EarI, Ksp632I

Bco5I, Bco116I,
CTCTT
M2.BstSEI
GACTC
4
m6A
GACTCTTCN[NNN]

BcoKI, BseZI,
C

Bst6I, Bsu6I,

CatHI, Eam1104I,

EarI, Ksp632I

Bco5I, Bco116I,
CTCTT
M2.Csu24377II, M2.Hpy298VII,
CCTC
3
m6A
CCTCTTCN[NNN]

BcoKI, BseZI,
C
M2.Hpy299VI, M2.Hpy30XII, M1.Hpy32VI,

Bst6I, Bsu6I,

M1.Hpy57VI, M2.Hpy66XIV, M1.Hpy99V,

CatHI, Eam1104I,

M1.HpyAVI, M1.HpyPVI, M2.HpyUM032VI,

EarI, Ksp632I

M.Mdi27140I, M2.MnlI

Bco5I, Bco116I,
CTCTT
M2.Eco31I
GGTCTC
4
m5C
GGTCTCTTCN[NNN]

BcoKI, BseZI,
C

Bst6I, Bsu6I,

CatHI, Eam1104I,

EarI, Ksp632I

Bli736I, BsaI,
GGTC
M.AbaBs12I, M.Awo1030I, M.Cdu23823I,
CCWGG
1, 5
m5C
CCWGGGTCTCN[NNNN],

Bso31I, BspTNI,
TC
M.Csa8155II, M.Cun9529I, M.EasL1Dcm,

CCWGGTCTCN[NNNN]

Bsu537I, Eco31I,

M.Eba57Dcm, M.Ecl34399Dcm,

EcoA4I, EcoO44I

M.Eco12761Dcm, M.Eco3609Dcm,

M.Eco4255Dcm, M.EcoVR50Dcm,

M.EsaSP1II, M.Kpn34618Dcm,

M.Kpn38547Dcm, M.Kpn39I, M.Kpn43816I,

M.Kpn677ORFDcm, M.Kpn928Dcm,

M.KpnNIH1Dcm, M.KpnNIH10Dcm,

M.KpnNIH30Dcm, M.Kva120Dcm, M.Lsp48II,

M.Pmi1I, M.SpaD2III, M.SplRp8II,

M.SptADcm, M.Tsu2489III

Bli736I, BsaI,
GGTC
M.AciI
GCGG
2, 4
m5C
GCGGGTCTCN[NNNN],

Bso31I, BspTNI,
TC

GCGGTCTCN[NNNN]

Bsu537I, Eco31I,

EcoA4I, EcoO44I

Bli736I, BsaI,
GGTC
M.AhdI
GACNNNN
2, 10
m6A
GACNNNNGGTCTCN[NNNN]

Bso31I, BspTNI,
TC

NGTC

Bsu537I, Eco31I,

EcoA4I, EcoO44I

Bli736I, BsaI,
GGTC
M.Ama55I, M.CnaPQ2III, M.Rmo1926II,
CGGCCG
1, 6
m5C
CGGCCGGTCTCN[NNNN]

Bso31I, BspTNI,
TC
M.SenC1765IV, M.SspG1I, M.XorPXII

Bsu537I, Eco31I,

EcoA4I, EcoO44I

Bli736I, BsaI,
GGTC
M.AquI
CYCGRG
1, 6
m5C
CYCGRGGTCTCN[NNNN],

Bso31I, BspTNI,
TC

CYCGGGTCTCN[NNNN]

Bsu537I, Eco31I,

EcoA4I, EcoO44I

Bli736I, BsaI,
GGTC
M.AspU41II, M.BisIII, M.BsuW23II,
CCWGG
2, 4
m5C
CCWGGTCTCN[NNNN]

Bso31I, BspTNI,
TC
M.CnaPQ2II, M.EcoO6Dcm, M.Eco12581Dcm,

Bsu537I, Eco31I,

M.Eco1655Dcm, M.Eco29787Dcm,

EcoA4I, EcoO44I

M.Eco3024Dcm, M.Eco3317Dcm,

M.Eco3325Dcm, M.Eco3740Dcm,

M.Eco3911Dcm, M.Eco4211Dcm,

M.Eco4465Dcm, M.Eco600Dcm,

M.Eco644Dcm, M.Eco86Dcm,

M.Eco9001Dcm, M.Eco9387Dcm,

M.EcoE1140Dcm, M.EcoE455Dcm,

M.EcoGDcm, M.EcoNU14Dcm, M.EcoRII,

M.Kpn223III, M.Kpn35657II, M.Kpn36Dcm,

M.Kpn555I, M.Kpn62629II, M.KpnAATV,

M.Kqu603Dcm, M.Psp345I, M.Sen158Dcm,

M.Sen641Dcm, M.SenA46Dcm,

M.SenAboDcm, M.TcoKWC4II

Bli736I, BsaI,
GGTC
M.AvaIX, M.Cfr10I, M.Nme18VI, M.VchAI,
RCCGGY
2, 5
m5C
RCCGGTCTCN[NNNN]

Bso31I, BspTNI,
TC
M.Vch0395I

Bsu537I, Eco31I,

EcoA4I, EcoO44I

Bli736I, BsaI,
GGTC
M.BcoSlacII, M.MspLW5III, M.Nbr128I,
CTCGAG
1, 6
m4C
CTCGAGGTCTCN[NNNN]

Bso31I, BspTNI,
TC
M.Psy18884II, M.Tam77409II, M.TbrGE1II,

Bsu537I, Eco31I,

M.TpaRLII

EcoA4I, EcoO44I

Bli736I, BsaI
GGTC
M.BepI, M.FpsJV, M.Gel16401III, M.Pdi8503I
CGCG
1, 4
m5C
CGCGGTCTCN[NNNN]

Bso31I, BspTNI,
TC

Bsu537I, Eco31I,

EcoA4I, EcoO44I

Bli736I, BsaI,
GGTC
M.Bhy27164IV, M.BstNI, M.EcoDHB4Dcm,
CCWGG
2, 4
m4C
CCWGGTCTCN[NNNN]

Bso31I, BspTNI,
TC
M.EcoK54Dcm, M.Gth3570I, M.Hla49239I,

Bsu537I, Eco31I,

M.MvaI, M.Pdi8503II, M.Sso30807Dcm,

EcoA4I, EcoO44I

M.YpeI, M.Ype41I, M.YpeShI

Bli736I, BsaI,
GGTC
M.BsoBI
CYCGRG
1, 6
m4C
CYCGRGGTCTCN[NNNN],

Bso31I, BspTNI,
TC

CYCGGGTCTCN[NNNN]

Bsu537I, Eco31I,

EcoA4I, EcoO44I

Bli736I, BsaI,
GGTC
M.BsuFI, M.CpeAIV, M.MspI
CCGG
1, 4
m5C
CCGGGTCTCN[NNNN], CCGGTCTCN[NNNN]

Bso31I, BspTNI,
TC

Bsu537I, Eco31I,

EcoA4I, EcoO44I

Bli736I, BsaI,
GGTC
M.Cab13497I, M.Dac11109III, M.Dco13527I,
CTAG
1, 4
m4C
CTAGGTCTCN[NNNN]

Bso31I, BspTNI,
TC
M.Djo10085I, M.Hbo11551I, M.Hka19301III,

Bsu537I, Eco31I,

M.Hme33500I, M.HsaR1I, M.Hsi33800I,

EcoA4I, EcoO44I

M.HspKM1WII, M.HspNII, M.HtuI, M.HvoDSI,

M.Lba2029II, M.Mbo45II, M.MjaI, M.MthZI,

M.Nas12278I, M.Rfl3010I, M.SsmMI

Bli736I, BsaI,
GGTC
M.Cdi630III
CCSSGG
2, 5
m4C
CCSSGGTCTCN[NNNN]

Bso31I, BspTNI,
TC

Bsu537I, Eco31I,

EcoA4I, EcoO44I

Bli736I, BsaI,
GGTC
M.Cfr9I, M.SmaI, M.Sma36365II, M.Xca85III,
CCCGGG
2, 5
m4C
CCCGGGTCTCN[NNNN]

Bso31I, BspTNI,
TC
M.XcyI, M.XmaI, M.XveII

Bsu537I, Eco31I,

EcoA4I, EcoO44I

Bli736I, BsaI,
GGTC
M.CfrBI, M.Sen1735IV, M.SenAnaIV
CCWWGG
1, 6
m4C
CCWWGGGTCTCN[NNNN],

Bso31I, BspTNI,
TC

CCWWGGTCTCN[NNNN]

Bsu537I, Eco31I,

EcoA4I, EcoO44I

Bli736I, BsaI
GGTC
M.Cmu51329I, M.EcoJA03PDcm,
CCWGG
1, 5
Unknown
CCWGGGTCTCN[NNNN],

Bso31I, BspTNI,
TC
M.RorR1Dcm, M.Sen8391Dcm, M.ZalSM2II

CCWGGTCTCN[NNNN]

Bsu537I, Eco31I,

EcoA4I, EcoO44I

Bli736I, BsaI,
GGTC
M.Cpa18696III, M.Ype19I, M.YpeDodI,
CCWGG
2, 4
Unknown
CCWGGTCTCN[NNNN]

Bso31I, BspTNI,
TC
M.YpeEDI, M.YpeJ9I, M.YpePGI

Bsu537I, Eco31I,

EcoA4I, EcoO44I

Bli736I, BsaI,
GGTC
M.DdeI
CTNAG
1, 5
m5C
CTNAGGTCTCN[NNNN]

Bso31I, BspTNI,
TC

Bsu537I, Eco31I,

EcoA4I, EcoO44I

Bli736I, BsaI,
GGTC
M.DsaV, M.Ecl18kI, M.FpsJVI, M.NlaX,
CCNGG
2, 4
m5C
CCNGGTCTCN[NNNN]

Bso31I, BspTNI,
TC
M.SenPI, M.SsoII

Bsu537I, Eco31I,

EcoA4I, EcoO44I

Bli736I, BsaI,
GGTC
M.Eco29kI, M.NgoAIII, M.SacII
CCGCGG
2, 5
m5C
CCGCGGTCTCN[NNNN]

Bso31I, BspTNI,
TC

Bsu537I, Eco31I,

EcoA4I, EcoO44I

Bli736I, BsaI,
GGTC
M.EcoNI
CCTNNNN
2, 10
m4C
CCTNNNNNAGGTCTCN[NNNN]

Bso31I, BspTNI,
TC

NAGG

Bsu537I, Eco31I,

EcoA4I, EcoO44I

Bli736I, BsaI,
GGTC
M.EfaRFII, M.Mae7806II
CCGG
2, 3
m4C
CCGGTCTCN[NNNN]

Bso31I, BspTNI,
TC

Bsu537I, Eco31I,

EcoA4I, EcoO44I

Bli736I, BsaI,
GGTC
M.FatI
CATG
1, 4
m5C
CATGGTCTCN[NNNN]

Bso31I, BspTNI,
TC

Bsu537I, Eco31I,

EcoA4I, EcoO44I

Bli736I, BsaI,
GGTC
M.FisAW1I, M.ThaI, M.Tph12270II
CGCG
1, 4
m4C
CGCGGTCTCN[NNNN]

Bso31I, BspTNI,
TC

Bsu537I, Eco31I,

EcoA4I, EcoO44I

Bli736I, BsaI,
GGTC
M.FnoB1II, M.Hal24586I, M.Hpy298V,
CCGG
1, 4
m4C
CCGGGTCTCN[NNNN], CCGGTCTCN[NNNN]

Bso31I, BspTNI,
TC
M.Hpy299IX, M.Hpy30V, M.Hpy32XI,

Bsu537I, Eco31I,

M.Hpy57V, M.Hpy66IX, M.Hpy99VIII,

EcoA4I, EcoO441

M.HpyFV, M.HpyUM032IX, M.HpyUM037IX,

M.MjaVI, M.PspJDRI, M.TmeBII

Bli736I, BsaI,
GGTC
M.FpsJI, M.HpaII, M.Nme18II, M.NmeAI
CCGG
2, 3
m5C
CCGGTCTCN[NNNN]

Bso31I, BspTNI,
TC

Bsu537I, Eco31I,

EcoA4I, EcoO44I

Bli736I, BsaI,
GGTC
M.Gel16401IV
CTGG
3
m5C
CTGGTCTCN[NNNN]

Bso31I, BspTNI,
TC

Bsu537I, Eco31I,

EcoA4I, EcoO44I

Bli736I, BsaI,
GGTC
M.GsuRed1IV, M.Osp807II, M.Tth111I
GACNNNG
2, 8
m6A
GACNNGGTCTCN[NNNN]

Bso31I, BspTNI,
TC

TC

Bsu537I, Eco31I,

EcoA4I, EcoO44I

Bli736I, BsaI
GGTC
M.Hpy298II, M.Hpy299II, M.Hpy66II,
ACNGT
2, 4
m4C
ACGGTCTCN[NNNN]

Bso31I, BspTNI,
TC
M.HpyUM032II, M.HpyUM037II

Bsu537I, Eco31I,

EcoA4I, EcoO44I

Bli736I, BsaI,
GGTC
M.Hpy30X, M.Hpy57VIII, M.HpyFXI, M.MhuI
CTNAG
1, 5
m4C
CTNAGGTCTCN[NNNN]

Bso31I, BspTNI,
TC

Bsu537I, Eco31I,

EcoA4I, EcoO44I

Bli736I, BsaI,
GGTC
M.Hpy99IV, M.HpyFORFX
CCNNGG
1, 6
m4C
CCNNGGGTCTCN[NNNN],

Bso31I, BspTNI,
TC

CCNNGGTCTCN[NNNN]

Bsu537I, Eco31I,

EcoA4I, EcoO44I

Bli736I, BsaI,
GGTC
M.HpyAV, M.Nme18IV
GAAGG
3, 4
m6A,
GAAGGTCTCN[NNNN]

Bso31I, BspTNI,
TC

m5C

Bsu537I, Eco31I,

EcoA4I, EcoO44I

Bli736I, BsaI,
GGTC
M.HpyD27I
GAGG
2, 4
m6A,
GAGGGTCTCN[NNNN],

Bso31I, BspTNI,
TC

m5C
GAGGTCTCN[NNNN]

Bsu537I, Eco31I,

EcoA4I, EcoO44I

Bli736I, BsaI,
GGTC
M.Lph145I, M.Lph93I
TCWCC
1
m6A
GGTCTCC[NNNN]

Bso31I, BspTNI,
TC

Bsu537I, Eco31I,

EcoA4I, EcoO44I

Bli736I, BsaI,
GGTC
M.Msp1II
CCAGG
5
m4C
CCAGGGTCTCN[NNNN],

Bso31I, BspTNI,
TC

CCAGGTCTCN[NNNN]

Bsu537I, Eco31I,

EcoA4I, EcoO44I

Bli736I, BsaI,
GGTC
M.Msp42II
CCWGG
1, 5
m4C
CCWGGGTCTCN[NNNN],

Bso31I, BspTNI,
TC

CCWGGTCTCN[NNNN]

Bsu537I, Eco31I,

EcoA4I, EcoO44I

Bli736I, BsaI,
GGTC
M.Nme18III
TCTGG
2, 4
m5C
TCTGGTCTCN[NNNN]

Bso31I, BspTNI,
TC

Bsu537I, Eco31I,

EcoA4I, EcoO44I

Bli736I, BsaI,
GGTC
M.NmeDI
VCCGGY
2, 5
m5C
VCCGGTCTCN[NNNN],

Bso31I, BspTNI,
TC

RCCGGGTCTCN[NNNN]

Bsu537I, Eco31I,

EcoA4I, EcoO44I

Bli736I, BsaI,
GGTC
M.NmeMC58III
TCTGG
5
m5C
TCTGGGTCTCN[NNNN],

Bso31I, BspTNI,
TC

TCTGGTCTCN[NNNN]

Bsu537I, Eco31I,

EcoA4I, EcoO44I

Bli736I, BsaI,
GGTC
M.Nsp209III, M.Nsp51109II
CTCGAG
1, 6
m5C
CTCGAGGTCTCN[NNNN]

Bso31I, BspTNI,
TC

Bsu537I, Eco31I,

EcoA4I, EcoO44I

Bli736I, BsaI,
GGTC
M.Pam7686I
CCATGG
1, 6
m5C
CCATGGGTCTCN[NNNN],

Bso31I, BspTNI,
TC

CCATGGTCTCN[NNNN]

Bsu537I, Eco31I,

EcoA4I, EcoO44I

Bli736I, BsaI,
GGTC
M.Pfa23572II
CGATCG
1, 6
m5C
CGATCGGTCTCN[NNNN]

Bso31I, BspTNI,
TC

Bsu537I, Eco31I,

EcoA4I, EcoO44I

Bli736I, BsaI,
GGTC
M.Pla5I
CTAG
1, 4
Unknown
CTAGGTCTCN[NNNN]

Bso31I, BspTNI,
TC

Bsu537I, Eco31I,

EcoA4I, EcoO44I

Bli736I, BsaI,
GGTC
M.Sam53668II
CCATGG
1, 6
Unknown
CCATGGGTCTCN[NNNN],

Bso31I, BspTNI,
TC

CCATGGTCTCN[NNNN]

Bsu537I, Eco31I,

EcoA4I, EcoO44I

Bli736I, BsaI,
GGTC
M.Sen1781III
CCWWGG
1, 6
Unknown
CCWWGGGTCTCN[NNNN],

Bso31I, BspTNI,
TC

CCWWGGTCTCN[NNNN]

Bsu537I, Eco31I,

EcoA4I, EcoO44I

Bli736I, BsaI,
GGTC
M.SonIII
GCANNNN
3, 9
m6A
GCANNNGGTCTCN[NNNN]

Bso31I, BspTNI,
TC

GTC

Bsu537I, Eco31I,

EcoA4I, EcoO44I

Bli736I, BsaI,
GGTC
M.TdeII
GAAGAG
5, 6
m6A,
GAAGAGGTCTCN[NNNN]

Bso31I, BspTNI,
TC

m4C

Bsu537I, Eco31I,

EcoA4I, EcoO44I

Bli736I, BsaI,
GGTC
M.TspRI
CASTG
1, 5
m5C
CASTGGTCTCN[NNNN]

Bso31I, BspTNI,
TC

Bsu537I, Eco31I,

EcoA4I, EcoO44I

Bli736I, BsaI,
GGTC
M1.AgoG2I, M2.AgoG2I
ACTGG
2, 5
m4C
ACTGGGTCTCN[NNNN],

Bso31I, BspTNI,
TC

ACTGGTCTCN[NNNN]

Bsu537I, Eco31I,

EcoA4I, EcoO44I

Bli736I, BsaI,
GGTC
M1.Bag18758II, M2.Bag18758II
CACGGG
3, 5
Unknown
CACGGGTCTCN[NNNN]

Bso31I, BspTNI,
TC

Bsu537I, Eco31I,

EcoA4I, EcoO44I

Bli736I, BsaI,
GGTC
M1.BcnI, M2.BcnI, M.NciI
CCSGG
2, 4
m4C
CCSGGTCTCN[NNNN]

Bso31I, BspTNI,
TC

Bsu537I, Eco31I,

EcoA4I, EcoO44I

Bli736I, BsaI,
GGTC
M1.BfiI
ACTGGG
5
m4C
ACTGGGTCTCN[NNNN]

Bso31I, BspTNI,
TC

Bsu537I, Eco31I,

EcoA4I, EcoO44I

Bli736I, BsaI,
GGTC
M1.BspACI
GCGG
4
m5C
GCGGGTCTCN[NNNN],

Bso31I, BspTNI,
TC

GCGGTCTCN[NNNN]

Bsu537I, Eco31I,

EcoA4I, EcoO44I

Bli736I, BsaI,
GGTC
M1.Hpy32II
TCYYC
1
m6A
GGTCTCC[NNNN]

Bso31I, BspTNI,
TC

Bsu537I, Eco31I,

EcoA4I, EcoO44I

Bli736I, BsaI,
GGTC
M1.LplAG30I
GAAGG
5
m5C
GAAGGGTCTCN[NNNN],

Bso31I, BspTNI,
TC

GAAGGTCTCN[NNNN]

Bsu537I, Eco31I,

EcoA4I, EcoO44I

Bli736I, BsaI,
GGTC
M2.BfuAI
GCAGGT
5 5
m5C
GCAGGTCTCN[NNNN]

Bso31I, BspTNI,
TC

Bsu537I, Eco31I,

EcoA4I, EcoO44I

Bli736I, BsaI,
GGTC
M2.HpyAVI, M1.MnlI
GAGG
4
m5C
GAGGGTCTCN[NNNN],

Bso31I, BspTNI,
TC

GAGGTCTCN[NNNN]

Bsu537I, Eco31I,

EcoA4I, EcoO44I

BsmBI, BstGZ53I,
CGTCT
M.AatII, M.Lsp6406III
GACGTC
2, 5
m6A
GACGTCTCN[NNNN]

Esp3I
C

BsmBI, BstGZ53I,
CGTCT
M.AflIII
ACRYGT
2, 5
m4C
ACRCGTCTCN[NNNN]

Esp3I
C

BsmBI, BstGZ53I,
CGTCT
M.AhdI
GACNNNN
2, 10
m6A
GACNNNNCGTCTCN[NNNN]

Esp3I
C

NGTC

BsmBI, BstGZ53I,
CGTCT
M.Ama55I, M.CnaPQ2III, M.Rmo1926II,
CGGCCG
1, 6
m5C
CGGCCGTCTCN[NNNN]

Esp3I
C
M.SenC1765IV, M.SspG1I, M.XorPXII

BsmBI, BstGZ53I,
CGTCT
M.AvaV, M.SliSI
GATC
1, 4
m4C
GATCGTCTCN[NNNN]

Esp3I
C

BsmBI, BstGZ53I,
CGTCT
M.BceSII
GGWCC
1, 5
m5C
GGWCCGTCTCN[NNNN]

Esp3I
C

BsmBI, BstGZ53I,
CGTCT
M.BepI, M.FpsJV, M.Gel16401III, M.Pdi8503I
CGCG
1, 4
m5C
CGCGTCTCN[NNNN]

Esp3I
C

BsmBI, BstGZ53I,
CGTCT
M.Bg12196III
CGTACG
2, 5
m4C
CGTACGTCTCN[NNNN]

Esp3I
C

BsmBI, BstGZ53I,
CGTCT
M.Bsp14III, M.Bsp3003I, M.Bsp3004I,
GATC
1, 4
m5C
GATCGTCTCN[NNNN]

Esp3I
C
M.CalB3III, M.CpeAIII, M.Esu35585I,

M.FpsJIV, M.Lba2031I, M.Lbo2004I,

M.LlaKR2I, M.Lmo1042I, M.Lmo7956II,

M.Lsp10393II, M.MspI7I, M.Sau3AI, M.Sba3I,

M.Sin395I, M.Spn23FI, M.Sth368I

BsmBI, BstGZ53I,
CGTCT
M.CagVIII
ACGCGT
2, 5
m4C
ACGCGTCTCN[NNNN]

Esp3I
C

BsmBI, BstGZ53I,
CGTCT
M.CocIII, M.Fsi4947II, M.TdeIII,
GGNCC
1, 5
m4C
GGNCCGTCTCN[NNNN]

Esp3I
C
M.Tph12270I

BsmBI, BstGZ53I,
CGTCT
M.CviJI
RGCB
2, 3
m5C
RGCGTCTCN[NNNN]

Esp3I
C

BsmBI, BstGZ53I,
CGTCT
M.Dac11109II, M.Hfo6591I, M.MhuII,
GTAC
1, 4
m4C
GTACGTCTCN[NNNN]

Esp3I
C
M.MjaV, M.MlaZII, M.MspLW5I, M.RsaI

BsmBI, BstGZ53I,
CGTCT
M.FisAW1I, M.ThaI, M.Tph12270II
CGCG
1, 4
m4C
CGCGTCTCN[NNNN]

Esp3I
C

BsmBI, BstGZ53I,
CGTCT
M.GsuRed1IV, M.Osp807II, M.Tth111I
GACNNNG
2, 8
m6A
GACNNCGTCTCN[NNNN]

Esp31
C

TC

BsmBI, BstGZ53I,
CGTCT
M.Hpy298II, M.Hpy299II, M.Hpy66II,
ACNGT
2, 4
m4C
ACCGTCTCN[NNNN]

Esp3I
C
M.HpyUM032II, M.HpyUM037II

BsmBI, BstGZ53I,
CGTCT
M.Hpy66I, M.Hpy99I
CGWCG
2, 4
m4C
CGWCGTCTCN[NNNN]

Esp3I
C

BsmBI, BstGZ53I,
CGTCT
M.Hpy99XI
ACGT
2, 3
m5C
ACGTCTCN[NNNN]

Esp3I
C

BsmBI, BstGZ53I,
CGTCT
M.Lph145I, M.Lph93I
TCWCC
1
m6A
CGTCTCC[NNNN]

Esp3I
C

BsmBI, BstGZ53I,
CGTCT
M.MjaII
GGNCC
1, 5
m5C
GGNCCGTCTCN[NNNN]

Esp3I
C

BsmBI, BstGZ53I,
CGTCT
M.NheI
GCTAGC
1, 6
m4C
GCTAGCGTCTCN[NNNN]

Esp3I
C

BsmBI, BstGZ53I,
CGTCT
M.Pfa23572II
CGATCG
1, 6
m5C
CGATCGTCTCN[NNNN]

Esp3I
C

BsmBI, BstGZ53I,
CGTCT
M.SonIII
GCANNNN
3, 9
m6A
GCANNNCGTCTCN[NNNN]

Esp3I
C

GTC

BsmBI, BstGZ53I,
CGTCT
M1.BceSIII
GCCGT
4
m4C
GCCGTCTCN[NNNN]

Esp3I
C

BsmBI, BstGZ53I,
CGTCT
M1.Dsh12II, M2.Dsh12II
GCCGTC
3, 4
m4C
GCCGTCTCN[NNNN]

Esp3I
C

BsmBI, BstGZ53I,
CGTCT
M1.HgaI, M2.LlaJI
GCGTC
2
m5C
GCGTCTCN[NNNN]

Esp3I
C

BsmBI, BstGZ53I,
CGTCT
M1.Hpy32II
TCYYC
1
m6A
CGTCTCC[NNNN]

Esp3I
C

BsmBI, BstGZ53I,
CGTCT
M2.BceSIII, M1.Pru9I, M2.Pru91
GCCGT
2, 4
m4C
GCCGTCTCN[NNNN]

Esp3I
C

BsmBI, BstGZ53I,
CGTCT
M2.HgaI, M1.LlaJI
GCGTC
3
m5C
GCGTCTCN[NNNN]

Esp3I
C

BsmBI, BstGZ53I,
CGTCT
M2.Nme579IV, M2.NmeMC58II
GCGTC
4
m6A
GCGTCTCN[NNNN]

Esp3I
C

BtgZI
GCGA
M.AbaBs12I, M.Awo1030I, M.Cdu23823I,
CCWGG
1, 5
m5C
CCWGGCGATGNNNNNNNNNN[NNNN]

TG
M.Csa8155II, M.Cun9529I, M.EasL1Dcm,

M.Eba57Dcm, M.Ec134399Dcm,

M.Eco12761Dcm, M.Eco3609Dcm,

M.Eco4255Dcm, M.EcoVR50Dcm,

M.EsaSP1II, M.Kpn34618Dcm,

M.Kpn38547Dcm, M.Kpn39I, M.Kpn43816I,

M.Kpn677ORFDcm, M.Kpn928Dcm,

M.KpnNIH1Dcm, M.KpnNIH10Dcm,

M.KpnNIH30Dcm, M.Kva120Dcm, M.Lsp48I,

M.Pmi1I, M.SpaD2III, M.SplRp8II,

M.SptADcm, M.Tsu2489III

BtgZI
GCGA
M.AciI
CCGC
1, 3
m5C
GCGGCGATGNNNNNNNNNN[NNNN],

TG

CCGCGATGNNNNNNNNNN[NNNN]

BtgZI
GCGA
M.Ama55I, M.CnaPQ2III, M.Rmo1926II,
CGGCCG
1, 6
m5C
CGGCCGCGATGNNNNNNNNNN[NNNN]

TG
M.SenC1765IV, M.SspG1I, M.XorPXII

BtgZI
GCGA
M.AquI
CYCGRG
1, 6
m5C
CYCGRGCGATGNNNNNNNNNN[NNNN]

TG

BtgZI
GCGA
M.AsiSI
GCGATCGC
2, 7
m5C
GCGATCGCGATGNNNNNNNNNN[NNNN]

TG

BtgZI
GCGA
M.AvaIX, M.Cfr10I, M.Nme18VI, M.VchAI,
RCCGGY
2, 5
m5C
RCCGGCGATGNNNNNNNNNN[NNNN]

TG
M.Vch0395I

BtgZI
GCGA
M.AvaV, M.SliSI
GATC
1, 4
m4C
GCGATGATCNNNNNNN[NNNN]

TG

BtgZI
GCGA
M.BamWS8I, M.CthV, M.EfaRFI, M.Saf8902II,
GCWGC
2, 4
m5C
GCWGCGATGNNNNNNNNNN[NNNN]

TG
M.TacII, M.TcoKWC4III

BtgZI
GCGA
M.BbvI, M.BceSIV
GCAGC
2, 4
m5C
GCTGCGATGNNNNNNNNNN[NNNN],

TG

GCAGCGATGNNNNNNNNNN[NNNN]

BtgZI
GCGA
M.BceSII
GGWCC
1, 5
m5C
GCGATGGWCCNNNNNN[NNNN]

TG

BtgZI
GCGA
M.BcoSlacII, M.MspLW5III, M.Nbr128I,
CTCGAG
1, 6
m4C
CTCGAGCGATGNNNNNNNNNN[NNNN]

TG
M.Psy18884II, M.Tam77409II, M.TbrGE1II,

M.TpaRLII

BtgZI
GCGA
M.BepI, M.FpsJV, M.Gel16401III, M.Pdi8503I
CGCG
1, 4
m5C
CGCGCGATGNNNNNNNNNN[NNNN],

TG

CGCGATGNNNNNNNNNN[NNNN]

BtgZI
GCGA
M.BfaSIII, M.Ccl10114I, M.CfrB38I,
ATGCAT
2, 5
m6A
GCGATGCATNNNNNNN[NNNN]

TG
M.CfrH1I, M.EcoGVI, M.EcoKII,

M.Fnu10562II, M.SbaUIV, M.Sbo12419III,

M.Sbo268II, M.Sen10384II, M.Sen10708II,

M.Sen1080II, M.Sen1175III, M.Sen13IV,

M.Sen13311IV, M.Sen1387II, M.Sen1427III,

M.Sen14882III, M.Sen15791III, M.Sen158III,

M.Sen16I, M.Sen1655III, M.Sen1676III,

M.Sen1677III, M.Sen1728III, M.Sen1735III,

M.Sen1736IV, M.Sen1764III, M.Sen1766III,

M.Sen1781IV, M.Sen1783III, M.Sen18569II,

M.Sen1878III, M.Sen1880III, M.Sen1896III,

M.Sen1898III, M.Sen1899III, M.Sen1903III,

M.Sen1906III, M.Sen1908III, M.Sen1910III,

M.Sen1921III, M.Sen1927II, M.Sen195II,

M.Sen2050II, M.Sen2064III, M.Sen2069III,

M.Sen22462I, M.Sen255II, M.Sen318III,

M.Sen3387III, M.Sen3890III, M.Sen483III,

M.Sen624III, M.Sen641III, M.Sen7378II,

M.Sen8391III, M.Sen8692III, M.SenA46III,

M.SenAbaII, M.SenAboII, M.SenAnaIII,

M.SenC1736III, M.SenC1765III,

M.SenC1808IV, M.SenC1810III, M.SenJIII,

M.SenL1II, M.SenL15III, M.SenN1660II,

M.SenSARA26II, M.SenSPBIII, M.SenTFIII

BtgZI
GCGA
M.BglI, M.CagI, M.DdeVCDI, M.GsuRed1I
GCCNNNN
2, 10
m4C
GCCNNNNNGGCGATGNNNNNNNNNN[NNNN]

TG

NGGC

BtgZI
GCGA
M.BsoBI
CYCGRG
1, 6
m4C
CYCGRGCGATGNNNNNNNNNN[NNNN]

TG

BtgZI
GCGA
M.Bsp14III, M.Bsp3003I, M.Bsp3004I,
GATC
1, 4
m5C
GCGATGATCNNNNNNN[NNNN]

TG
M.CalB3III, M.CpeAIII, M.Esu35585I,

M.FpsJIV, M.Lba2031I, M.Lbo2004I,

M.LlaKR2I, M.Lmo1042I, M.Lmo7956II,

M.Lsp10393II, M.MspI7I, M.Sau3AI, M.Sba3I,

M.Sin395I, M.Spn23FI, M.Sth368I

BtgZI
GCGA
M.BstXI
CCANNNN
3, 10
m6A
CCANNGCGATGGNNNNNNNNN[NNNN]

TG

NNTGG

BtgZI
GCGA
M.BsuFI, M.CpeAIV, M.MspI
CCGG
1, 4
m5C
CCGGCGATGNNNNNNNNNN[NNNN]

TG

BtgZI
GCGA
M.Cab13497I, M.Dac11109III, M.Dco13527I,
CTAG
1, 4
m4C
CTAGCGATGNNNNNNNNNN[NNNN]

TG
M.Djo10085I, M.Hbo11551I, M.Hka19301III,

M.Hme33500I, M.HsaR1I, M.Hsi33800I,

M.HspKM1WII, M.HspNII, M.HtuI, M.HvoDSI,

M.Lba2029II, M.Mbo45II, M.MjaI, M.MthZI,

M.Nas12278I, M.Rfl3010I, M.SsmMI

BtgZI
GCGA
M.Cac8I
GCNNGC
2, 5
m5C
GCNNGCGATGNNNNNNNNNN[NNNN]

TG

BtgZI
GCGA
M.CcrNAV
YGCCGGCR
3, 6
m5C
YGCCGGCGATGNNNNNNNNNN[NNNN]

TG

BtgZI
GCGA
M.Cdi630IV, M.Ckr177III, M.CmaLM2II,
GCNGC
2, 4
m5C
GCNGCGATGNNNNNNNNNN[NNNN]

TG
M.CocII, M.Fnu4HI, M.Fsp4HI

BtgZI
GCGA
M.CfrBI, M.Sen1735IV, M.SenAnaIV
CCWWGG
1, 6
m4C
CCWWGGCGATGNNNNNNNNNN[NNNN]

TG

BtgZI
GCGA
M.Cmu51329I, M.EcoJA03PDcm,
CCWGG
1, 5
Unknown
CCWGGCGATGNNNNNNNNNN[NNNN]

TG
M.RorR1Dcm, M.Sen8391Dcm, M.ZalSM2II

BtgZI
GCGA
M.CocIII, M.Fsi4947II, M.TdeIII,
GGNCC
1, 5
m4C
GCGATGGNCCNNNNNN[NNNN]

TG
M.Tph12270I

BtgZI
GCGA
M.CsiFSMII, M.MfoCT6IV, M.NgoAIV,
GCCGGC
2, 5
m5C
GCCGGCGATGNNNNNNNNNN[NNNN]

TG
M.NgoMIV, M1.Sgr13350II

BtgZI
GCGA
M.CviJI
RGCB
2, 3
m5C
RGCGATGNNNNNNNNNN[NNNN]

TG

BtgZI
GCGA
M.CviRI, M.Hpy298VIII, M.Hpy299VIII,
TGCA
1, 4
m6A
GCGATGCANNNNNNNN[NNNN]

TG
M.Hpy30VI, M.Hpy32V, M.Hpy57II,

M.Hpy66VIII, M.HpyFVI, M.HpyUM032VIII,

M.HpyUM037VIII

BtgZI
GCGA
M.Dac11109II, M.Hfo6591I, M.MhuII,
GTAC
1, 4
m4C
GCGATGTACNNNNNNN[NNNN]

TG
M.MjaV, M.MlaZII, M.MspLW5I, M.RsaI

BtgZI
GCGA
M.DdeI
CTNAG
1, 5
m5C
CTNAGCGATGNNNNNNNNNN[NNNN]

TG

BtgZI
GCGA
M.Esp1396I
CCANNNN
3, 9
m6A
CCANGCGATGGNNNNNNNNN[NNNN]

TG

NTGG

BtgZI
GCGA
M.FatI
CATG
1, 4
m5C
CATGCGATGNNNNNNNNNN[NNNN]

TG

BtgZI
GCGA
M.FisAW1I, M.ThaI, M.Tph12270II
CGCG
1, 4
m4C
CGCGCGATGNNNNNNNNNN[NNNN],

TG

CGCGATGNNNNNNNNNN[NNNN]

BtgZI
GCGA
M.FnoB1II, M.Hal24586I, M.Hpy298V,
CCGG
1, 4
m4C
CCGGCGATGNNNNNNNNNN[NNNN]

TG
M.Hpy299IX, M.Hpy30V, M.Hpy32XI,

M.Hpy57V, M.Hpy66IX, M.Hpy99VIII,

M.HpyFV, M.HpyUM032IX, M.HpyUM037IX,

M.MjaVI, M.PspJDRI, M.TmeBII

BtgZI
GCGA
M.HhaI, M.HinP1I, M.Hpy99III, M.HpyAVIII,
GCGC
2, 3
m5C
GCGCGATGNNNNNNNNNN[NNNN]

TG
M.HpyUM032XV

BtgZI
GCGA
M.Hka19301IV
TCGCGA
2, 5
m4C
TCGCGATGNNNNNNNNNN[NNNN]

TG

BtgZI
GCGA
M.Hme33500II
MGCWGCD
5
m4C
MGCWGCGATGNNNNNNNNNN[NNNN]

TG

BtgZI
GCGA
M.Hpy30VIII, M.Hpy32VIII, M.Hpy57III,
TCNNGA
1, 6
m6A
TCGCGATGNNNNNNNNNN[NNNN]

TG
M.Hpy66VI, M.Hpy99XVIII, M.HpyFVIII,

M.HpyPVIII

BtgZI
GCGA
M.Hpy30X, M.Hpy57VIII, M.HpyFXI, M.MhuI
CTNAG
1, 5
m4C
CTNAGCGATGNNNNNNNNNN[NNNN]

TG

BtgZI
GCGA
M.Hpy99IV, M.HpyFORFX
CCNNGG
1, 6
m4C
CCNNGGCGATGNNNNNNNNNN[NNNN]

TG

BtgZI
GCGA
M.HpyD27I
GAGG
2, 4
m6A,
GAGGCGATGNNNNNNNNNN[NNNN]

TG

m5C

BtgZI
GCGA
M.MjaII
GGNCC
1, 5
m5C
GCGATGGNCCNNNNNN[NNNN]

TG

BtgZI
GCGA
M.Msp1II
CCAGG
5
m4C
CCAGGCGATGNNNNNNNNNN [NNNN]

TG

BtgZI
GCGA
M.Msp42II
CCWGG
1, 5
m4C
CCWGGCGATGNNNNNNNNNN[NNNN]

TG

BtgZI
GCGA
M.NheI
GCTAGC
1, 6
m4C
GCTAGCGATGNNNNNNNNNN[NNNN],

TG

GCGATGCTAGCNNNNN[NNNN]

BtgZI
GCGA
M.Nme18V
GCGCGC
2, 5
m5C
GCGCGCGATGNNNNNNNNNN[NNNN]

TG

BtgZI
GCGA
M.NmeDI
VCCGGY
2, 5
m5C
VCCGGCGATGNNNNNNNNNN[NNNN]

TG

BtgZI
GCGA
M.NmeMC58III
TCTGG
5
m5C
TCTGGCGATGNNNNNNNNNN[NNNN]

TG

BtgZI
GCGA
M.Nsp209III, M.Nsp51109II
CTCGAG
1, 6
m5C
CTCGAGCGATGNNNNNNNNNN[NNNN]

TG

BtgZI
GCGA
M.NspI, M.NspHI
RCATGY
2, 5
m5C
RCATGCGATGNNNNNNNNNN[NNNN]

TG

BtgZI
GCGA
M.Pam7686I
CCATGG
1, 6
m5C
CCATGGCGATGNNNNNNNNNN[NNNN]

TG

BtgZI
GCGA
M.Pfa23572II
CGATCG
1, 6
m5C
CGATCGCGATGNNNNNNNNNN[NNNN]

TG

BtgZI
GCGA
M.Pla5I
CTAG
1, 4
Unknown
CTAGCGATGNNNNNNNNNN[NNNN]

TG

BtgZI
GCGA
M.Sam53668II
CCATGG
1, 6
Unknown
CCATGGCGATGNNNNNNNNNN[NNNN]

TG

BtgZI
GCGA
M.Sen1781III
CCWWGG
1, 6
Unknown
CCWWGGCGATGNNNNNNNNNN[NNNN]

TG

BtgZI
GCGA
M.SonIII
GACNNNN
2, 8
m6A
GACGCGATGCNNNNNNNNN[NNNN]

TG

TGC

BtgZI
GCGA
M.TdeII
GAAGAG
5, 6
m6A,
GAAGAGCGATGNNNNNNNNNN[NNNN]

TG

m4C

BtgZI
GCGA
M.TspRI
CASTG
1, 5
m5C
CASTGCGATGNNNNNNNNNN[NNNN]

TG

BtgZI
GCGA
M.XcmI
CCANNNN
3, 13
m6A
CCANNNNNGCGATGGNNNNNNNNN[NNNN]

TG

NNNNNTG

G

BtgZI
GCGA
M.Yen2516I
GCGCGC
2, 5
Unkno
GCGCGCGATGNNNNNNNNNN[NNNN]

TG

wn

BtgZI
GCGA
M1.AgoG2I, M2.AgoG2I
ACTGG
2, 5
m4C
ACTGGCGATGNNNNNNNNNN[NNNN]

TG

BtgZI
GCGA
M1.BccI, M1.Hpy30XI, M2.Hpy66X,
GATGG
2
m6A
GCGATGGNNNNNNNNN[NNNN]

TG
M1.Jsp2502I, M2.MmyCVI

BtgZI
GCGA
M1.BhaI, M1.Bst19I, M.Fnu10562III,
GATGC
3
m6A
GCGATGCNNNNNNNNN[NNNN]

TG
M1.Mbo45IV, M2.Mbo45IV

BtgZI
GCGA
M1.BspACI
GCGG
4
m5C
GCGGCGATGNNNNNNNNNN[NNNN]

TG

BtgZI
GCGA
M1.Csp16704IV, M2.Csp16704IV,
GATGC
2, 3
m6A
GCGATGCNNNNNNNNN[NNNN]

TG
M.Lsp10393I, M.Mha183II, M.Mha185II,

M.Mha807II, M.Mva1312II

BtgZI
GCGA
M1.HgaI, M2.LlaJI
GACGC
4
m5C
GACGCGATGNNNNNNNNNN[NNNN]

TG

BtgZI
GCGA
M1.Hpy299X, M2.Hpy32XIII, M1.HpyFXIII,
GATGG
2, 3
m6A
GCGATGGNNNNNNNNN[NNNN]

TG
M2.HpyFXIII, M2.HpyUM037VI, M1.McaCI,

M2.McaCI, M1.PspHUN102II,

M2.PspHUN102II

BtgZI
GCGA
M1.LplAG30I
GAAGG
5
m5C
GAAGGCGATGNNNNNNNNNN[NNNN]

TG

BtgZI
GCGA
M1.Sgr13350III, M2.Sgr13350III
GAAGAGC
5, 6
m6A,
GAAGAGCGATGNNNNNNNNNN[NNNN]

TG

m4C

BtgZI
GCGA
M2.BccI, M2.Hpy299X, M1.Hpy66X,
GATGG
3
m6A
GCGATGGNNNNNNNNN[NNNN]

TG
M1.HpyC1I, M2.HpyC1I, M1.HpyUM032X,

M2.Jsp2502I, M1.MmyCVI

BtgZI
GCGA
M2.BceSIII, M1.Pru9I, M2.Pru9I
ACGGC
2, 4
m4C
ACGGCGATGNNNNNNNNNN[NNNN]

TG

BtgZI
GCGA
M2.BhaI
GATGC
2
m6A
GCGATGCNNNNNNNNN[NNNN]

TG

BtgZI
GCGA
M2.HpyAVI, M1.MnlI
GAGG
4
m5C
GAGGCGATGNNNNNNNNNN[NNNN]

TG

RleAI
CCCAC
M.AflIII
ACRYGT
2, 5
m4C
CCCACAYGTNNNNNN[NNN]

A

RleAI
CCCAC
M.AluBI
AGCT
1, 4
m6A
CCCACAGCTNNNNNN[NNN]

A

RleAI
CCCAC
M.ApaI
GGGCCC
3, 4
m5C
GGGCCCACANNNNNNNNN[NNN]

A

RleAI
CCCAC
M.AspDUT2II, M.BstYI, M.CagIII,
RGATCY
2, 5
m4C
RGATCCCACANNNNNNNNN[NNN]

A
M.Mpa1757V, M.Mru1279V, M.RsaIII,

M.XhoII

RleAI
CCCAC
M.AspWA102I, M.AvaVII, M.BceJII, M.CbeI,
GGCC
2, 3
m4C
GGCCCACANNNNNNNNN[NNN]

A
M.Ckr177II, M.CmaLM2IV, M.CpeAVII,

M.Csp323II, M.PhoI, M.Ssp6714III, M.SuaI

RleAI
CCCAC
M.Asu14238I, M.Bhy27164III, M.CagIX,
CATG
2, 3
m6A
CCCACATGNNNNNNN[NNN]

A
M.Cje11351II, M.CviAII, M.CviQVII,

M.Fli6794III, M.Gli15749I, M.HauIII, M.HpyI,

M.Hpy166IV, M.Hpy188II, M.Hpy298I,

M.Hpy299I, M.Hpy30I, M.Hpy32I, M.Hpy57I,

M.Hpy66XI, M.Hpy99X, M.HpyAI, M.HpyFI,

M.HpyPI, M.HpyUM032I, M.HpyUM037I,

M.Lsp48II, M.LspLP1II, M.NlaIII, M.NsaI,

M.Sba3II, M.SdeAIII, M.SspT436I, M.ThaIV,

M.TvoI

RleAI
CCCAC
M.AvaV, M.SliSI
GATC
1, 4
m4C
GATCCCACANNNNNNNNN[NNN]

A

RleAI
CCCAC
M.BamHI
GGATCC
2, 5
m4C
GGATCCCACANNNNNNNNN[NNN]

A

RleAI
CCCAC
M.BanII, M.EcoT38I
GRGCYC
3, 4
m5C
GRGCCCACANNNNNNNNN[NNN]

A

RleAI
CCCAC
M.BanLII, M.CpeAII, M.SinI
GGWCC
2, 4
m5C
GGWCCCACANNNNNNNNN[NNN]

A

RleAI
CCCAC
M.BceSII
GGWCC
1, 5
m5C
GGWCCCCACANNNNNNNNN[NNN],

A

GGWCCCACANNNNNNNNN[NNN]

RleAI
CCCAC
M.BglI, M.CagI, M.DdeVCDI, M.GsuRed1I
GCCNNNN
2, 10
m4C
GCCCACANGGCNNNNN[NNN]

A

NGGC

RleAI
CCCAC
M.BhaII, M.BspRI, M.BsuRI, M.CthVI,
GGCC
2, 3
m5C
GGCCCACANNNNNNNNN[NNN]

A
M.DthLII, M.HaeIII, M.MthTI, M.NgoAII,

M.NgoPII, M.Pod15391I

RleAI
CCCAC
M.Bsp14III, M.Bsp3003I, M.Bsp3004I,
GATC
1, 4
m5C
GATCCCACANNNNNNNNN[NNN]

A
M.CalB3III, M.CpeAIII, M.Esu35585I,

M.FpsJIV, M.Lba2031I, M.Lbo2004I,

M.LlaKR2I, M.Lmo1042I, M.Lmo7956II,

M.Lsp10393II, M.MspI7I, M.Sau3AI, M.Sba3I,

M.Sin395I, M.Spn23FI, M.Sth368I

RleAI
CCCAC
M.BstXI
CCANNNN
3, 10
m6A
CCCACANNNNTGGNN[NNN]

A

NNTGG

RleAI
CCCAC
M.CocIII, M.Fsi4947II, M.TdeIII,
GGNCC
1, 5
m4C
GGNCCCCACANNNNNNNNN[NNN],

A
M.Tph12270I

GGNCCCACANNNNNNNNN[NNN],

GGCCCACANNNNNNNNN[NNN]

RleAI
CCCAC
M.CviJI
RGCB
2, 3
m5C
VGCCCACANNNNNNNNN[NNN]

A

RleAI
CCCAC
M.Dac11109II, M.Hfo6591I, M.MhuII,
GTAC
1, 4
m4C
GTACCCACANNNNNNNNN[NNN]

A
M.MjaV, M.MlaZII, M.MspLW5I, M.RsaI

RleAI
CCCAC
M.Djo10085II, M.Ssp6803II
GGCC
2, 3
Unknown
GGCCCACANNNNNNNNN[NNN]

A

RleAI
CCCAC
M.EcoO109I
RGGNCCY
3, 5
m5C
RGGNCCCACANNNNNNNNN[NNN]

A

RleAI
CCCAC
M.EcoVIII, M.HindIII, M.Lad60222I, M.LlaCI
AAGCTT
1, 6
m6A
CCCACAAGCTTNNNN[NNN]

A

RleAI
CCCAC
M.Esp1396I
CCANNNN
3, 9
m6A
CCCACANNNTGGNNN[NNN]

A

NTGG

RleAI
CCCAC
M.FatI
CATG
1, 4
m5C
CCCACATGNNNNNNN[NNN]

A

RleAI
CCCAC
M.HgiCI
GGYRCC
2, 5
m5C
GGYRCCCACANNNNNNNNN[NNN]

A

RleAI
CCCAC
M.Hpy298II, M.Hpy299II, M.Hpy66II,
ACNGT
2, 4
m4C
CCCACAGTNNNNNNN[NNN]

A
M.HpyUM032II, M.HpyUM037II

RleAI
CCCAC
M.HtuII, M.Nas12278II
CATTC
2
m6A
CCCACATTCNNNNNN[NNN]

A

RleAI
CCCAC
M.Mae7806III, M.PspPI, M.Sau96I
GGNCC
2, 4
m5C
GGNCCCACANNNNNNNNN[NNN],

A

GGCCCACANNNNNNNNN[NNN]

RleAI
CCCAC
M.MjaII
GGNCC
1, 5
m5C
GGNCCCCACANNNNNNNNN[NNN],

A

GGNCCCACANNNNNNNNN[NNN],

GGCCCACANNNNNNNNN[NNN]

RleAI
CCCAC
M.MpeII
RGCY
2, 3
m5C
RGCCCACANNNNNNNNN[NNN]

A

RleAI
CCCAC
M.NgoAXV, M.NgoMV
GGNNCC
2, 5
m5C
GGNNCCCACANNNNNNNNN[NNN],

A

GGNCCCACANNNNNNNNN[NNN]

RleAI
CCCAC
M.NheI
GCTAGC
1, 6
m4C
GCTAGCCCACANNNNNNNNN[NNN]

A

RleAI
CCCAC
M.NspI, M.NspHI
RCATGY
2, 5
m5C
CCCACATGYNNNNNN[NNN]

A

RleAI
CCCAC
M.Psp737I
CCCCCD
4
m4C
CCCCCACANNNNNNNNN[NNN]

A

RleAI
CCCAC
M.SbaUII
CAGCTG
2, 5
m6A
CCCACAGCTGNNNNN[NNN]

A

RleAI
CCCAC
M.SuaII
RGATCY
2, 5
m5C
RGATCCCACANNNNNNNNN[NNN]

A

RleAI
CCCAC
M.TspRI
CASTG
1, 5
m5C
CCCACASTGNNNNNN[NNN]

A

RleAI
CCCAC
M1.CspNS6II
TCCGCC
5
m5C
TCCGCCCACANNNNNNNNN[NNN]

A

RleAI
CCCAC
M2.BstF5I
CATCC
2
m6A
CCCACATCCNNNNNN[NNN]

A

RleAI
CCCAC
M4.BstF5I, M1.Cin755I, M2.Cin755I,
CATCC
2, 3
m6A
CCCACATCCNNNNNN[NNN]

A
M1.CmeSR3I, M2.CmeSR3I, M1.Csp12AI,

M1.Csp323I, M2.Csp323I, M1.Csp410I,

M2.Csp410I, M.FokI, M1.Msp315I,

M2.Msp315I, M.Sau13435I, M.StsI,

M1.Tsu2489IV, M2.Tsu2489IV,

M3.Tsu2489IV

AarI
CACCT
M.AbaBs12I, M.Awo1030I, M.Cdu23823I,
CCWGG
1, 5
m5C
CACCTGCCWGG[NNNN]

GC
M.Csa8155II, M.Cun9529I, M.EasL1Dcm,

M.Eba57Dcm, M.Ecl34399Dcm,

M.Eco12761Dcm, M.Eco3609Dcm,

M.Eco4255Dcm, M.EcoVR50Dcm,

M.EsaSP1II, M.Kpn34618Dcm,

M.Kpn38547Dcm, M.Kpn39I, M.Kpn43816I,

M.Kpn677ORFDcm, M.Kpn928Dcm,

M.KpnNIH1Dcm, M.KpnNIH10Dcm,

M.KpnNIH30Dcm, M.Kva120Dcm, M.Lsp48I,

M.Pmi1I, M.SpaD2III, M.SplRp8II,

M.SptADcm, M.Tsu2489III

AarI
CACCT
M.Acc65VI, M.SenAZIII
CTKMAG
2, 5
m6A
CACCTGCAGNN[NNNN]

GC

AarI
CACCT
M.AciI
CCGC
1, 3
m5C
CACCTGCGGNN[NNNN],

GC

CACCTGCCGCN[NNNN]

AarI
CACCT
M.Ade56712I, M.Asp588III, M.AveCB51II,
CTGCAG
2, 5
m6A
CACCTGCAGNN[NNNN]

GC
M.BbrUIII, M.Blo30698I, M.BsuBI,

M.CpaYL7III, M.Dba7044I, M.Dfa45958II,

M.Eco12581I, M.Eco2012I, M.EcoGIII,

M.EcoG089I, M.Eho16691II, M.Hal5920II,

M.Lsp6406II, M.MspNR41II, M.Pae490I,

M.Pox23570I, M.PstI, M.RpaII, M.Sca3403II,

M.Tsp61I, M.XmnII, M.YenWI, M.YinY228I

AarI
CACCT
M.AplI
CTGCAG
3, 4
m5C
CACCTGCAGNN[NNNN]

GC

AarI
CACCT
M.AvaIX, M.Cfr10I, M.Nme18VI, M.VchAI,
RCCGGY
2, 5
m5C
CACCTGCCGGY[NNNN]

GC
M.Vch0395I

AarI
CACCT
M.AvaV, M.SliSI
GATC
1, 4
m4C
GATCACCTGCNNNN[NNNN]

GC

AarI
CACCT
M.BamWS8I, M.CthV, M.EfaRFI, M.Saf8902II,
GCWGC
2, 4
m5C
CACCTGCWGCN[NNNN]

GC
M.TacII, M.TcoKWC4III

AarI
CACCT
M.BbvI, M.BceSIV
GCAGC
2, 4
m5C
CACCTGCTGCN[NNNN],

GC

CACCTGCAGCN[NNNN]

AarI
CACCT
M.BceSII
GGWCC
1, 5
m5C
GGWCCACCTGCNNNN[NNNN]

GC

AarI
CACCT
M.BepI, M.FpsJV, M.Gel16401III, M.Pdi8503I
CGCG
1, 4
m5C
CACCTGCGCGN[NNNN]

GC

AarI
CACCT
M.Bsp14III, M.Bsp3003I, M.Bsp3004I,
GATC
1, 4
m5C
GATCACCTGCNNNN[NNNN]

GC
M.CalB3III, M.CpeAIII, M.Esu35585I,

M.FpsJIV, M.Lba2031I, M.Lbo2004I,

M.LlaKR2I, M.Lmo1042I, M.Lmo7956II,

M.Lsp10393II, M.MspI7I, M.Sau3AI, M.Sba3I,

M.Sin395I, M.Spn23FI, M.Sth368I

AarI
CACCT
M.BspRAC04I, M.EcaI, M.Eco3936IV
GGTNACC
3, 5
m6A
GGTCACCTGCNNNN[NNNN]

GC

AarI
CACCT
M.BstXI
CCANNNN
3, 10
m6A
CCACCTGCNTGG[NNNN]

GC

NNTGG

AarI
CACCT
M.BsuFI, M.CpeAIV, M.MspI
CCGG
1, 4
m5C
CACCTGCCGGN[NNNN]

GC

AarI
CACCT
M.Cab13497I, M.Dac11109III, M.Dco13527I,
CTAG
1, 4
m4C
CACCTGCTAGN[NNNN]

GC
M.Djo10085I, M.Hbo11551I, M.Hka19301III,

M.Hme33500I, M.HsaR1I, M.Hsi33800I,

M.Hsp KM1WII, M.HspNII, M.HtuI, M.HvoDSI,

M.Lba2029II, M.Mbo45II, M.MjaI, M.MthZI,

M.Nas12278I, M.Rfl3010I, M.SsmMI

AarI
CACCT
M.Cac8I
GCNNGC
2, 5
m5C
CACCTGCNNGC[NNNN]

GC

AarI
CACCT
M.Cdi630IV, M.Ckr177III, M.CmaLM2II,
GCNGC
2, 4
m5C
CACCTGCNGCN[NNNN]

GC
M.CocII, M.Fnu4HI, M.Fsp4HI

AarI
CACCT
M.Cmu51329I, M.EcoJA03PDcm,
CCWGG
1, 5
Unkno
CACCTGCCWGG[NNNN]

GC
M.RorR1Dcm, M.Sen8391Dcm, M.ZalSM2II

wn

AarI
CACCT
M.CocIII, M.Fsi4947II, M.TdeIII,
GGNCC
1, 5
m4C
GGNCCACCTGCNNNN[NNNN]

GC
M.Tph12270I

AarI
CACCT
M.CsiFSMII, M.MfoCT6IV, M.NgoAIV,
GCCGGC
2, 5
m5C
CACCTGCCGGC[NNNN]

GC
M.NgoMIV, M1.Sgr13350II

AarI
CACCT
M.CviRI, M.Hpy298VIII, M.Hpy299VIII,
TGCA
1, 4
m6A
TGCACCTGCNNNN[NNNN],

GC
M.Hpy30VI, M.Hpy32V, M.Hpy57II,

CACCTGCANNN[NNNN]

M.Hpy66VIII, M.HpyFVI, M.HpyUM032VIII,

M.HpyUM037VIII

AarI
CACCT
M.Dac11109II, M.Hfo6591I, M.MhuII,
GTAC
1, 4
m4C
GTACACCTGCNNNN[NNNN]

GC
M.MjaV, M.MlaZII, M.MspLW5I, M.RsaI

AarI
CACCT
M.DdeI
CTNAG
1, 5
m5C
CACCTGCTNAG[NNNN]

GC

AarI
CACCT
M.DfeI, M.Fnu419II, M.Pen113II
CTNNAG
2, 5
m6A
CACCTGCAGNN[NNNN]

GC

AarI
CACCT
M.Esp1396I
CCANNNN
3, 9
m6A
CCACCTGCTGGN[NNNN]

GC

NTGG

AarI
CACCT
M.FatI
CATG
1, 4
m5C
CACCTGCATGN[NNNN]

GC

AarI
CACCT
M.FisAW1I, M.ThaI, M.Tph12270II
CGCG
1, 4
m4C
CACCTGCGCGN[NNNN]

GC

AarI
CACCT
M.FnoB1II, M.Hal24586I, M.Hpy298V,
CCGG
1, 4
m4C
CACCTGCCGGN[NNNN]

GC
M.Hpy299IX, M.Hpy30V, M.Hpy32XI,

M.Hpy57V, M.Hpy66IX, M.Hpy99VIII,

M.HpyFV, M.HpyUM032IX, M.HpyUM037IX,

M.MjaVI, M.PspJDRI, M.TmeBII

AarI
CACCT
M.HgiCI
GGYRCC
2, 5
m5C
GGCACCTGCNNNN[NNNN]

GC

AarI
CACCT
M.HhaI, M.HinP1I, M.Hpy99III, M.HpyAVIII,
GCGC
2, 3
m5C
CACCTGCGCNN[NNNN]

GC
M.HpyUM032XV

AarI
CACCT
M.Hme33500II
HGCWGCK
3
m4C
CACCTGCWGCK[NNNN]

GC

AarI
CACCT
M.Hpy30IX, M.Hpy32IX, M.Hpy57IX,
GTNNAC
2, 5
m6A
GTNCACCTGCNNNN[NNNN]

GC
M.Hpy66XIII, M.Hpy8I, M.HpyFIX,

M.HpyUM032XI, M.HpyUM037V, M.MjaIV,

M.MspRAC08II

AarI
CACCT
M.Hpy30X, M.Hpy57VIII, M.HpyFXI, M.MhuI
CTNAG
1, 5
m4C
CACCTGCTNAG[NNNN]

GC

AarI
CACCT
M.Hpy57X
CTRYAG
2, 5
m6A
CACCTGCAGNN[NNNN]

GC

AarI
CACCT
M.Hpy99II, M.Tsp45I, M.Tsu2489II
GTSAC
2, 4
m6A
GTCACCTGCNNNN[NNNN]

GC

AarI
CACCT
M.HpyD27I
CCTC
1, 3
m5C,
CACCTGCCTCN[NNNN]

GC

m6A

AarI
CACCT
M.MjaII
GGNCC
1, 5
m5C
GGNCCACCTGCNNNN[NNNN]

GC

AarI
CACCT
M.Msp1II
CCTGG
1
m4C
CACCTGCCTGG[NNNN]

GC

AarI
CACCT
M.Msp42II
CCWGG
1, 5
m4C
CACCTGCCWGG[NNNN]

GC

AarI
CACCT
M.NgoAXV, M.NgoMV
GGNNCC
2, 5
m5C
GGCACCTGCNNNN[NNNN]

GC

AarI
CACCT
M.NheI
GCTAGC
1, 6
m4C
GCTAGCACCTGCNNNN[NNNN],

GC

CACCTGCTAGC[NNNN]

AarI
CACCT
M.Nme18V
GCGCGC
2, 5
m5C
CACCTGCGCGC[NNNN]

GC

AarI
CACCT
M.NmeDI
VCCGGY
2, 5
m5C
CACCTGCCGGB[NNNN]

GC

AarI
CACCT
M.NmeMC58III
CCAGA
1
m5C
CACCTGCCAGA[NNNN]

GC

AarI
CACCT
M.NspI, M.NspHI
RCATGY
2, 5
m5C
CACCTGCATGY [NNNN]

GC

AarI
CACCT
M.Pla5I
CTAG
1, 4
Unknown
CACCTGCTAGN[NNNN]

GC

AarI
CACCT
M.SonIII
GACNNNN
2, 8
m6A
GACCACCTGCNNNN[NNNN]

GC

TGC

AarI
CACCT
M.TspRI
CASTG
1, 5
m5C
CACCTGCASTG[NNNN]

GC

AarI
CACCT
M.Yen2516I
GCGCGC
2, 5
Unknown
CACCTGCGCGC[NNNN]

GC

AarI
CACCT
M1.AgoG2I, M2.AgoG2I
CCAGT
1, 4
m4C
CACCTGCCAGT[NNNN]

GC

AarI
CACCT
M1.BspACI
CCGC
1
m5C
CACCTGCCGCN[NNNN]

GC

AarI
CACCT
M1.HgaI, M2.LlaJI
GCGTC
2
m5C
CACCTGCGTCN[NNNN]

GC

AarI
CACCT
M1.HphI
TCACC
2
m5C
TCACCTGCNNNN[NNNN]

GC

AarI
CACCT
M1.LplAG30I
CCTTC
1
m5C
CACCTGCCTTC[NNNN]

GC

AarI
CACCT
M2.BceSIII, M1.Pru9I, M2.Pru9I
GCCGT
2, 4
m4C
CACCTGCCGTN[NNNN]

GC

AarI
CACCT
M2.HpyAVI, M1.MnlI
CCTC
1
m5C
CACCTGCCTCN[NNNN]

GC

BspQI, LguI, PciSI,
GCTCT
M.AbaBs12I, M.Awo1030I, M.Cdu23823I,
CCWGG
1, 5
m5C
CCWGGCTCTTCN[NNN]

SapI, VpaK32I
TC
M.Csa8155II, M.Cun9529I, M.EasL1Dcm,

M.Eba57Dcm, M.Ec134399Dcm,

M.Eco12761Dcm, M.Eco3609Dcm,

M.Eco4255Dcm, M.EcoVR50Dcm,

M.EsaSP1II, M.Kpn34618Dcm,

M.Kpn38547Dcm, M.Kpn39I, M.Kpn43816I,

M.Kpn677ORFDcm, M.Kpn928Dcm,

M.KpnNIH1Dcm, M.KpnNIH10Dcm,

M.KpnNIH30Dcm, M.Kva120Dcm, M.Lsp48I,

M.Pmi1I, M.SpaD2III, M.SplRp8II,

M.SptADcm, M.Tsu2489III

BspQI, LguI, PciSI,
GCTCT
M.AciI
CCGC
1, 3
m5C
GCGGCTCTTCN[NNN], CCGCTCTTCN[NNN]

SapI, VpaK32I
TC

BspQI, LguI, PciSI,
GCTCT
M.AluBI
AGCT
1, 4
m6A
AGCTCTTCN[NNN]

SapI, VpaK32I
TC

BspQI, LguI, PciSI,
GCTCT
M.AluI, M.CspCY2I
AGCT
2, 3
m5C
AGCTCTTCN[NNN]

SapI, VpaK32I
TC

BspQI, LguI, PciSI,
GCTCT
M.Ama55I, M.CnaPQ2III, M.Rmo1926II,
CGGCCG
1, 6
m5C
CGGCCGCTCTTCN[NNN]

SapI, VpaK32I
TC
M.SenC1765IV, M.SspG1I, M.XorPXII

BspQI, LguI, PciSI,
GCTCT
M.AquI
CYCGRG
1, 6
m5C
CYCGRGCTCTTCN[NNN]

SapI, VpaK32I
TC

BspQI, LguI, PciSI,
GCTCT
M.AsiSI
GCGATCGC
2, 7
m5C
GCGATCGCTCTTCN[NNN]

SapI, VpaK32I
TC

BspQI, LguI, PciSI,
GCTCT
M.AspJHL3I, M2.Psp32OWII
GAGCTC
3, 4
m4C
GAGCTCTTCN[NNN]

SapI, VpaK32I
TC

BspQI, LguI, PciSI,
GCTCT
M.AvaIX, M.Cfr10I, M.Nme18VI, M.VchAI,
RCCGGY
2, 5
m5C
RCCGGCTCTTCN[NNN]

SapI, VpaK32I
TC
M.Vch0395I

BspQI, LguI, PciSI,
GCTCT
M.BamWS8I, M.CthV, M.EfaRFI, M.Saf8902II,
GCWGC
2, 4
m5C
GCWGCTCTTCN[NNN]

SapI, VpaK32I
TC
M.TacII, M.TcoKWC4III

BspQI, LguI, PciSI,
GCTCT
M.BanII, M.EcoT38I
GRGCYC
3, 4
m5C
GRGCTCTTCN[NNN]

SapI, VpaK32I
TC

BspQI, LguI, PciSI,
GCTCT
M.BbvI, M.BceSIV
GCAGC
2, 4
m5C
GCTGCTCTTCN[NNN],

SapI, VpaK32I
TC

GCAGCTCTTCN[NNN]

BspQI, LguI, PciSI,
GCTCT
M.BcoSlacII, M.MspLW5III, M.Nbr128I,
CTCGAG
1, 6
m4C
CTCGAGCTCTTCN[NNN]

SapI, VpaK32I
TC
M.Psy18884II, M.Tam77409II, M.TbrGE1II,

M.TpaRLII

BspQI, LguI, PciSI,
GCTCT
M.BepI, M.FpsJV, M.Gel16401III, M.Pdi8503I
CGCG
1, 4
m5C
CGCGCTCTTCN[NNN]

SapI, VpaK32I
TC

BspQI, LguI, PciSI,
GCTCT
M.BglI, M.CagI, M.DdeVCDI, M.GsuRed1I
GCCNNNN
2, 10
m4C
GCCNNNNNGGCTCTTCN[NNN]

SapI, VpaK32I
TC

NGGC

BspQI, LguI, PciSI,
GCTCT
M.Ble402III, M.Psp10HI
SAGCTS
3, 4
m4C
SAGCTCTTCN[NNN]

SapI, VpaK32I
TC

BspQI, LguI, PciSI,
GCTCT
M.BsoBI
CYCGRG
1, 6
m4C
CYCGRGCTCTTCN[NNN]

SapI, VpaK32I
TC

BspQI, LguI, PciSI,
GCTCT
M.BsuFI, M.CpeAIV, M.MspI
CCGG
1, 4
m5C
CCGGCTCTTCN[NNN]

SapI, VpaK32I
TC

BspQI, LguI, PciSI,
GCTCT
M.Cab13497I, M.Dac11109III, M.Dco13527I,
CTAG
1, 4
m4C
CTAGCTCTTCN[NNN]

SapI, VpaK32I
TC
M.Djo10085I, M.Hbo11551I, M.Hka19301III,

M.Hme33500I, M.HsaR1I, M.Hsi33800I,

M.HspKM1WII, M.HspNII, M.HtuI, M.HvoDSI,

M.Lba2029II, M.Mbo45II, M.MjaI, M.MthZI,

M.Nas12278I, M.Rfl3010I, M.SsmMI

BspQI, LguI, PciSI,
GCTCT
M.Cac8I
GCNNGC
2, 5
m5C
GCNNGCTCTTCN[NNN]

SapI, VpaK32I
TC

BspQI, LguI, PciSI,
GCTCT
M.Cdi630IV, M.Ckr177III, M.CmaLM2II,
GCNGC
2, 4
m5C
GCNGCTCTTCN[NNN]

SapI, VpaK32I
TC
M.CocII, M.Fnu4HI, M.Fsp4HI

BspQI, LguI, PciSI,
GCTCT
M.CfrBI, M.Sen1735IV, M.SenAnaIV
CCWWGG
1, 6
m4C
CCWWGGCTCTTCN[NNN]

SapI, VpaK32I
TC

BspQI, LguI, PciSI,
GCTCT
M.Cmu51329I, M.EcoJA03PDcm,
CCWGG
1, 5
Unknown
CCWGGCTCTTCN[NNN]

SapI, VpaK32I
TC
M.RorR1Dcm, M.Sen8391Dcm, M.ZalSM2II

BspQI, LguI, PciSI,
GCTCT
M.CsiFSMII, M.MfoCT6IV, M.NgoAIV,
GCCGGC
2, 5
m5C
GCCGGCTCTTCN[NNN]

SapI, VpaK32I
TC
M.NgoMIV, M1.Sgr13350II

BspQI, LguI, PciSI,
GCTCT
M.CviJI
RGCB
2, 3
m5C
VGCTCTTCN[NNN]

SapI, VpaK32I
TC

BspQI, LguI, PciSI,
GCTCT
M.DdeI
CTNAG
1, 5
m5C
CTNAGCTCTTCN[NNN]

SapI, VpaK32I
TC

BspQI, LguI, PciSI,
GCTCT
M.FatI
CATG
1, 4
m5C
CATGCTCTTCN[NNN]

SapI, VpaK32I
TC

BspQI, LguI, PciSI,
GCTCT
M.FisAW1I, M.ThaI, M.Tph12270II
CGCG
1, 4
m4C
CGCGCTCTTCN[NNN]

SapI, VpaK32I
TC

BspQI, LguI, PciSI,
GCTCT
M.FnoB1II, M.Hal24586I, M.Hpy298V,
CCGG
1, 4
m4C
CCGGCTCTTCN[NNN]

SapI, VpaK32I
TC
M.Hpy299IX, M.Hpy30V, M.Hpy32XI,

M.Hpy57V, M.Hpy66IX, M.Hpy99VIII,

M.HpyFV, M.HpyUM032IX, M.HpyUM037IX,

M.MjaVI, M.PspJDRI, M.TmeBII

BspQI, LguI, PciSI,
GCTCT
M.HhaI, M.HinP1I, M.Hpy9911I, M.HpyAVIII,
GCGC
2, 3
m5C
GCGCTCTTCN[NNN]

SapI, VpaK32I
TC
M.HpyUM032XV

BspQI, LguI, PciSI,
GCTCT
M.Hme33500II
MGCWGCD
5
m4C
MGCWGCTCTTCN[NNN]

SapI, VpaK32I
TC

BspQI, LguI, PciSI,
GCTCT
M.Hpy30X, M.Hpy57VIII, M.HpyFXI, M.MhuI
CTNAG
1, 5
m4C
CTNAGCTCTTCN[NNN]

SapI, VpaK32I
TC

BspQI, LguI, PciSI,
GCTCT
M.Hpy99IV, M.HpyFORFX
CCNNGG
1, 6
m4C
CCNNGGCTCTTCN[NNN]

SapI, VpaK32I
TC

BspQI, LguI, PciSI,
GCTCT
M.HpyD27I
GAGG
2, 4
m6A,
GAGGCTCTTCN[NNN]

SapI, VpaK32I
TC

m5C

BspQI, LguI, PciSI,
GCTCT
M.Mma5219II, M.TarMY3I
AGCT
2, 3
m4C
AGCTCTTCN[NNN]

SapI, VpaK32I
TC

BspQI, LguI, PciSI,
GCTCT
M.MpeII
RGCY
2, 3
m5C
RGCTCTTCN[NNN]

SapI, VpaK32I
TC

BspQI, LguI, PciSI,
GCTCT
M.Msp1II
CCAGG
5
m4C
CCAGGCTCTTCN[NNN]

SapI, VpaK32I
TC

BspQI, LguI, PciSI,
GCTCT
M.Msp42II
CCWGG
1, 5
m4C
CCWGGCTCTTCN[NNN]

SapI, VpaK32I
TC

BspQI, LguI, PciSI,
GCTCT
M.NgoAI
RGCGCY
3, 4
m5C
RGCGCTCTTCN[NNN]

SapI, VpaK32I
TC

BspQI, LguI, PciSI,
GCTCT
M.NheI
GCTAGC
1, 6
m4C
GCTAGCTCTTCN[NNN]

SapI, VpaK32I
TC

BspQI, LguI, PciSI,
GCTCT
M.Nme18V
GCGCGC
2, 5
m5C
GCGCGCTCTTCN[NNN]

SapI, VpaK32I
TC

BspQI, LguI, PciSI,
GCTCT
M.NmeDI
VCCGGY
2, 5
m5C
VCCGGCTCTTCN[NNN]

SapI, VpaK32I
TC

BspQI, LguI, PciSI,
GCTCT
M.NmeMC58III
TCTGG
5
m5C
TCTGGCTCTTCN[NNN]

SapI, VpaK32I
TC

BspQI, LguI, PciSI,
GCTCT
M.Nsp209III, M.Nsp51109II
CTCGAG
1, 6
m5C
CTCGAGCTCTTCN[NNN]

SapI, VpaK32I
TC

BspQI, LguI, PciSI,
GCTCT
M.NspI, M.NspHI
RCATGY
2, 5
m5C
RCATGCTCTTCN[NNN]

SapI, VpaK32I
TC

BspQI, LguI, PciSI,
GCTCT
M.Pae10332II, M1.Pae50071I,
CAGCTC
3, 4
m4C
CAGCTCTTCN[NNN]

SapI, VpaK32I
TC
M2.Pae50071I

BspQI, LguI, PciSI,
GCTCT
M.Pam7686I
CCATGG
1, 6
m5C
CCATGGCTCTTCN[NNN]

SapI, VpaK32I
TC

BspQI, LguI, PciSI,
GCTCT
M.Pfa23572II
CGATCG
1, 6
m5C
CGATCGCTCTTCN[NNN]

SapI, VpaK32I
TC

BspQI, LguI, PciSI,
GCTCT
M.Pla5I
CTAG
1, 4
Unknown
CTAGCTCTTCN[NNN]

SapI, VpaK32I
TC

BspQI, LguI, PciSI,
GCTCT
M.SacI, M.Sgr13350I
GAGCTC
3, 4
m5C
GAGCTCTTCN[NNN]

SapI, VpaK32I
TC

BspQI, LguI, PciSI,
GCTCT
M.Sam53668II
CCATGG
1, 6
Unknown
CCATGGCTCTTCN[NNN]

SapI, VpaK32I
TC

BspQI, LguI, PciSI,
GCTCT
M.Sen1781III
CCWWGG
1, 6
Unknown
CCWWGGCTCTTCN[NNN]

SapI, VpaK32I
TC

BspQI, LguI, PciSI,
GCTCT
M.Tam77409IV
CCRCTC
4
m4C
CCGCTCTTCN[NNN]

SapI, VpaK32I
TC

BspQI, LguI, PciSI,
GCTCT
M.TspRI
CASTG
1, 5
m5C
CASTGCTCTTCN[NNN]

SapI, VpaK32I
TC

BspQI, LguI, PciSI,
GCTCT
M.XmnI
GAANNNN
2, 9
m6A
GAAGCTCTTCN[NNN]

SapI, VpaK32I
TC

TTC

BspQI, LguI, PciSI,
GCTCT
M.Yen2516I
GCGCGC
2, 5
Unknown
GCGCGCTCTTCN[NNN]

SapI, VpaK32I
TC

BspQI, LguI, PciSI,
GCTCT
M1.AgoG2I, M2.AgoG2I
ACTGG
2, 5
m4C
ACTGGCTCTTCN[NNN]

SapI, VpaK32I
TC

BspQI, LguI, PciSI,
GCTCT
M1.BspACI
GCGG
4
m5C
GCGGCTCTTCN[NNN]

SapI, VpaK32I
TC

BspQI, LguI, PciSI,
GCTCT
M1.HgaI, M2.LlaJI
GACGC
4
m5C
GACGCTCTTCN[NNN]

SapI, VpaK32I
TC

BspQI, LguI, PciSI,
GCTCT
M1.LplAG30I
GAAGG
5
m5C
GAAGGCTCTTCN[NNN]

SapI, VpaK32I
TC

BspQI, LguI, PciSI,
GCTCT
M2.BceSIII, M1.Pru9I, M2.Pru9I
ACGGC
2, 4
m4C
ACGGCTCTTCN[NNN]

SapI, VpaK32I
TC

BspQI, LguI, PciSI,
GCTCT
M2.HpyAVI, M1.MnlI
GAGG
4
m5C
GAGGCTCTTCN[NNN]

SapI, VpaK32I
TC

Use of Other Types of Methyltransferases

Despite using type II DNA methlyase enzymes in the examples, type I restriction-modification enzymes may also be considered. Type I restriction-modification enzymes are multi-subunit enzymes that have both methyltransferase and restriction endonuclease activity. They are usually consists of three types of subunits encoded by three different genes: methylation subunit, restriction subunit and DNA sequence-recognition subunit (specificity subunit). Both the methylation subunit and specificity subunit are required for methyltransferase activity.

For example, M.EcoCI5I is known to recognize GACNNNNNNRTAAY (methylated base/opposite base in bold, reverse complementary RTTAYNNNNNNGTC). A methylation-protectable restriction site according to the invention could be provided using this sequence to protect an overlapping BsaI site: RTTAYNNNNNGGTCTC (methylated base/opposite base in bold, BsaI recognition sequence underlined, M.EcoCI5I recognition sequence in italic). An E. coli strain expressing both the methylation subunit M.EcoCI5I and the specificity subunit S.EcoCI5I may be able to methylate the corresponding bases in the cut control element, which would block the methylation-protected BsaI site.

Table 4 provides some further example restriction enzyme and type I restriction-modification enzyme combinations, or recognition sequences thereof, that may be provided in accordance with the invention. The skilled person will understand that these are examples and not an exhaustive list of potential combinations that would be encompassed by the claims.

TABLE 4

Type I

Posi-

restric-

tion

tion-

of

Overlapping

Restric-
Restric-
modifi-

methyl-
Type of
methylation/

tion
tion
cation
Methylation
ated
methyl-
restriction

enzyme
site
enzyme
sequence
base
ation
site

AlwXI,
GCAG
M.AspU
GGCANNNN
4, 11
m6A
GGCAGCNNNNTGAN

BbvI,
C
41III
NNTGA

[NNNN]

BseKI,

BseXI,

Bsp42

3I,

Bst12I,

Bst71I,

BstV1I,

GeolCI,

Lsp11

09I

AlwXI,
GCAG
M.Sth3
GCANNNNN
3, 11
m6A
GCAGCNNNNNTCG

BbvI,
C
01I
NNTCG

[NNNN]

BseKI,

BseXI,

Bsp42

3I,

Bst12I,

Bst71I,

BstV1I,

GeolCI,

Lsp11

09I

StsI
GGAT
M.Bme
GAGNNNNN
2, 11
m6A
GAGNNNNGGATGGNNNNN

G
D34I
NNTGG

NNNN[NNNN]

BpmI,
CTGGA
M.Cba1
GAGNNNNN
2, 10
m6A
CTGGAGNNNNNRTGTNNN

GsuI
G
31
RTGT

NN[NN]

BsmBI,
CGTCT
M.SmaF
CAYNNNNNN
2, 10
m6A
CAYNNCGTCTCA[NNNN]

BstGZ
C
1I
TCA

53I,

Esp3I

RdeGB
ACCCA
M.Lal21
CCAANNNNN
4, 13
m6A
GCACCCAGNNNTTGGNNN

II
G
61
NNNTGC

NNNNNNNN[NN],

CCAACCCAGNNNTGCNNNN

NNNNNNNN[NN]

Example Vector Sequences

The sequences of plasmids for scarless Metclo are listed below in fasta format. The underlined sequence is the “discard sequence” containing the LacZ selection fragment and a replication origin for high copy vector DNA preparation. The [Image Omitted] is the “recessive/truncating composite element” that contains a “methylation-protectable element” and a “nonprotectable element” arranged head-to-head at appropriate distance for adapter switching. The rest of the sequence is the vector backbone containing a low copy replication origin (p15A based) and a selection marker (either kanamycin or spectinomycin).

For BsaI/M.Osp807II based scarless Metclo, the plasmids are:

pMXLK_VL
,
pMXLK_VM
,

pMXLK_VR
⁢

(
kanamycin
)

⁢

pMXLS_VL
,
pMXLS_VM
,

pMXLS_VR
⁢

(
spectinomycin
)

For LguI/M.XmnI based scarless Metclo, the plasmids are:

pMZLK_VL
,
pMZLK_VM
,

pMZLK_VR
⁢

(
kanamycin
)

⁢

pMZLS_VL
,
pMZLS_VM
,

pMZLS_VR
⁢

(
spectinomycin
)

The sequences of plasmids for standard 4-fragment assembly of 1 kb DNA and 2-stage assembly of ˜200 kb DNA from 28 fragments are listed in fasta format as well. The underlined sequence for 1 kb assembly vectors contain LacZ selection marker. The underlined sequence for 200 kb assembly vectors contain LacZ selection marker and a replication origin for high copy vector DNA preparation. The rest of the sequences are vector backbones.

For 1 kb assembly, the plasmids are:

pMXP_A4E
,
pMYP_A5E
,
pMZP_P6T

For 200 kb assembly, the plasmids are:

pMXBG_A7I
,
pMXBG_I7G
,
pMXBG_G7F
,
pMXBG_F7E
,

pMXBK_A7E

Such vector sequences are applicable for the vectors of the examples of the invention, with the specific truncating, maintained or insertional composite elements exchanged as appropriate. A skilled person familiar with molecular cloning can re-construct the vectors herein by PCR from the commercially available Moclo kit.

>pMXLK_VL

(SEQ ID NO: 28)

[Image Omitted]

GTCAGACCAAGTTTACTCAT

ATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAG

GTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTT

TTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTT

GAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCA

CCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTT

TCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTC

TAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCT

ACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGA

TAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGG

CGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAG

CGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAG

CGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCA

GGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGG

TATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATT

TTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACG

CGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTC

TTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGA

GTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAG

TGAGCGAGGAAGCGGATGAGCGCCCAATACGCAAACCGCCTCTCCCCGCG

CGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAA

AGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCATTAGG

CACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGAATT

GTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGC

CAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGC

TCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGG

CGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGC

GTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGC

CTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTG

CGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATG

CCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCC

TGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGAC

[Image Omitted]

CCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGA

GACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATG

GCTAAAATGAGAATATCACCGGAATTGAAAAAACTGATCGAAAAATACCG

CTGCGTAAAAGATACGGAAGGAATGTCTCCTGCTAAGGTATATAAGCTGG

TGGGAGAAAATGAAAACCTATATTTAAAAATGACGGACAGCCGGTATAAA

GGGACCACCTATGATGTGGAACGGGAAAAGGACATGATGCTATGGCTGGA

AGGAAAGCTGCCTGTTCCAAAGGTCCTGCACTTTGAACGGCATGATGGCT

GGAGCAATCTGCTCATGAGTGAGGCCGATGGCGTCCTTTGCTCGGAAGAG

TATGAAGATGAACAAAGCCCTGAAAAGATTATCGAGCTGTATGCGGAGTG

CATCAGGCTCTTTCACTCCATCGACATATCGGATTGTCCCTATACGAATA

GCTTAGACAGCCGCTTAGCCGAATTGGATTACTTACTGAATAACGATCTG

GCCGATGTGGATTGCGAAAACTGGGAAGAGGACACTCCATTTAAAGATCC

GCGCGAGCTGTATGATTTTTTAAAGACGGAAAAGCCCGAAGAGGAACTTG

TCTTTTCCCACGGCGACCTGGGAGACAGCAACATCTTTGTGAAAGATGGC

AAAGTAAGTGGCTTTATTGATCTTGGGAGAAGCGGCAGGGCGGACAAGTG

GTATGACATTGCCTTCTGCGTCCGGTCGCTCAGGGAGGATATCGGGGAAG

AACAGTATGTCGAGCTATTTTTTGACTTACTGGGGATCAAGCCTGATTGG

GAGAAAATAAAATATTATATTTTACTGGATGAATTGTTTTAGGAGCCTGT

TTAATAAGATGATCTTCTTGAGATCGTTTTGGTCTGCGCGTAATCTCTTG

CTCTGAAAACGAAAAAACCGCCTTGCAGGGCGGTTTTTCGAAGGTTCTCT

GAGCTACCAACTCTTTGAACCGAGGTAACTGGCTTGGAGGAGCGCAGTCA

CCAAAACTTGTCCTTTCAGTTTAGCCTTAACCGGCGCATGACTTCAAGAC

TAACTCCTCTAAATCAATTACCAGTGGCTGCTGCCAGTGGTGCTTTTGCA

TGTCTTTCCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCG

GTCGGACTGAACGGGGGGTTCGTGCATACAGTCCAGCTTGGAGCGAACTG

CCTACCCGGAACTGAGTGTCAGGCGTGGAATGAGACAAACGCGGCCATAA

CAGCGGAATGACACCGGTAAACCGAAAGGCAGGAACAGGAGAGCGCACGA

GGGAGCCGCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTT

CGCCACCACTGATTTGAGCGTCAGATTTCGTGATGCTTGTCAGGGGGGCG

GAGCCTATGGAAAAACGGCTTTGCCGCGGCCCTCTCACTTCCCTGTTAAG

TATCTTCCTGGCATCTTCCAGGAAATCTCCGCCCCGTTCGTAAGCCATTT

CCGCTCGCCGCAGTCGAACGACCGAGCGTAGCGAGTCAGTGAGCGAGGAA

GCGGAATATATCCTGTATCACATATTCTGCTGACGCACCGGTGCAGCCTT

TTTTCTCCTGCCACATGAAGCACTTCACTGACACCCTCATCAGTGCCAAC

ATAGTAAGCCAGTATACACTCCGCTAGCGCGCAAGCGGCCGCA

>pMXLK_VM

(SEQ ID NO: 29)

[Image Omitted]

GTCAGACCAAGTTTACTCAT

ATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAG

GTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTT

TTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTT

GAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCA

CCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTT

TCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTC

TAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCT

ACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGA

TAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGG

CGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAG

CGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAG

CGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCA

GGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGG

TATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATT

TTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACG

CGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTC

TTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGA

GTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAG

TGAGCGAGGAAGCGGATGAGCGCCCAATACGCAAACCGCCTCTCCCCGCG

CGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAA

AGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCATTAGG

CACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGAATT

GTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGC

CAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGC

TCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGG

CGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGC

GTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGC

CTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTG

CGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATG

CCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCC

TGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGAC

[Image Omitted]

CCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGA

GACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATG

GCTAAAATGAGAATATCACCGGAATTGAAAAAACTGATCGAAAAATACCG

CTGCGTAAAAGATACGGAAGGAATGTCTCCTGCTAAGGTATATAAGCTGG

TGGGAGAAAATGAAAACCTATATTTAAAAATGACGGACAGCCGGTATAAA

GGGACCACCTATGATGTGGAACGGGAAAAGGACATGATGCTATGGCTGGA

AGGAAAGCTGCCTGTTCCAAAGGTCCTGCACTTTGAACGGCATGATGGCT

GGAGCAATCTGCTCATGAGTGAGGCCGATGGCGTCCTTTGCTCGGAAGAG

TATGAAGATGAACAAAGCCCTGAAAAGATTATCGAGCTGTATGCGGAGTG

CATCAGGCTCTTTCACTCCATCGACATATCGGATTGTCCCTATACGAATA

GCTTAGACAGCCGCTTAGCCGAATTGGATTACTTACTGAATAACGATCTG

GCCGATGTGGATTGCGAAAACTGGGAAGAGGACACTCCATTTAAAGATCC

GCGCGAGCTGTATGATTTTTTAAAGACGGAAAAGCCCGAAGAGGAACTTG

TCTTTTCCCACGGCGACCTGGGAGACAGCAACATCTTTGTGAAAGATGGC

AAAGTAAGTGGCTTTATTGATCTTGGGAGAAGCGGCAGGGCGGACAAGTG

GTATGACATTGCCTTCTGCGTCCGGTCGCTCAGGGAGGATATCGGGGAAG

AACAGTATGTCGAGCTATTTTTTGACTTACTGGGGATCAAGCCTGATTGG

GAGAAAATAAAATATTATATTTTACTGGATGAATTGTTTTAGGAGCCTGT

TTAATAAGATGATCTTCTTGAGATCGTTTTGGTCTGCGCGTAATCTCTTG

CTCTGAAAACGAAAAAACCGCCTTGCAGGGCGGTTTTTCGAAGGTTCTCT

GAGCTACCAACTCTTTGAACCGAGGTAACTGGCTTGGAGGAGCGCAGTCA

CCAAAACTTGTCCTTTCAGTTTAGCCTTAACCGGCGCATGACTTCAAGAC

TAACTCCTCTAAATCAATTACCAGTGGCTGCTGCCAGTGGTGCTTTTGCA

TGTCTTTCCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCG

GTCGGACTGAACGGGGGGTTCGTGCATACAGTCCAGCTTGGAGCGAACTG

CCTACCCGGAACTGAGTGTCAGGCGTGGAATGAGACAAACGCGGCCATAA

CAGCGGAATGACACCGGTAAACCGAAAGGCAGGAACAGGAGAGCGCACGA

GGGAGCCGCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTT

CGCCACCACTGATTTGAGCGTCAGATTTCGTGATGCTTGTCAGGGGGGCG

GAGCCTATGGAAAAACGGCTTTGCCGCGGCCCTCTCACTTCCCTGTTAAG

TATCTTCCTGGCATCTTCCAGGAAATCTCCGCCCCGTTCGTAAGCCATTT

CCGCTCGCCGCAGTCGAACGACCGAGCGTAGCGAGTCAGTGAGCGAGGAA

GCGGAATATATCCTGTATCACATATTCTGCTGACGCACCGGTGCAGCCTT

TTTTCTCCTGCCACATGAAGCACTTCACTGACACCCTCATCAGTGCCAAC

ATAGTAAGCCAGTATACACTCCGCTAGCGCGCAAGCGGCCGCA

>pMXLK_VR

(SEQ ID NO: 30)

[Image Omitted]

GTCAGACCAAGTTTACTCAT

ATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAG

GTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTT

TTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTT

GAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCA

CCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTT

TCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTC

TAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCT

ACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGA

TAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGG

CGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAG

CGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAG

CGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCA

GGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGG

TATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATT

TTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACG

CGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTC

TTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGA

GTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAG

TGAGCGAGGAAGCGGATGAGCGCCCAATACGCAAACCGCCTCTCCCCGCG

CGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAA

AGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCATTAGG

CACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGAATT

GTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGC

CAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGC

TCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGG

CGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGC

GTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGC

CTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTG

CGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATG

CCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCC

TGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGAC

[Image Omitted]

CCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGA

GACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATG

GCTAAAATGAGAATATCACCGGAATTGAAAAAACTGATCGAAAAATACCG

CTGCGTAAAAGATACGGAAGGAATGTCTCCTGCTAAGGTATATAAGCTGG

TGGGAGAAAATGAAAACCTATATTTAAAAATGACGGACAGCCGGTATAAA

GGGACCACCTATGATGTGGAACGGGAAAAGGACATGATGCTATGGCTGGA

AGGAAAGCTGCCTGTTCCAAAGGTCCTGCACTTTGAACGGCATGATGGCT

GGAGCAATCTGCTCATGAGTGAGGCCGATGGCGTCCTTTGCTCGGAAGAG

TATGAAGATGAACAAAGCCCTGAAAAGATTATCGAGCTGTATGCGGAGTG

CATCAGGCTCTTTCACTCCATCGACATATCGGATTGTCCCTATACGAATA

GCTTAGACAGCCGCTTAGCCGAATTGGATTACTTACTGAATAACGATCTG

GCCGATGTGGATTGCGAAAACTGGGAAGAGGACACTCCATTTAAAGATCC

GCGCGAGCTGTATGATTTTTTAAAGACGGAAAAGCCCGAAGAGGAACTTG

TCTTTTCCCACGGCGACCTGGGAGACAGCAACATCTTTGTGAAAGATGGC

AAAGTAAGTGGCTTTATTGATCTTGGGAGAAGCGGCAGGGCGGACAAGTG

GTATGACATTGCCTTCTGCGTCCGGTCGCTCAGGGAGGATATCGGGGAAG

AACAGTATGTCGAGCTATTTTTTGACTTACTGGGGATCAAGCCTGATTGG

GAGAAAATAAAATATTATATTTTACTGGATGAATTGTTTTAGGAGCCTGT

TTAATAAGATGATCTTCTTGAGATCGTTTTGGTCTGCGCGTAATCTCTTG

CTCTGAAAACGAAAAAACCGCCTTGCAGGGCGGTTTTTCGAAGGTTCTCT

GAGCTACCAACTCTTTGAACCGAGGTAACTGGCTTGGAGGAGCGCAGTCA

CCAAAACTTGTCCTTTCAGTTTAGCCTTAACCGGCGCATGACTTCAAGAC

TAACTCCTCTAAATCAATTACCAGTGGCTGCTGCCAGTGGTGCTTTTGCA

TGTCTTTCCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCG

GTCGGACTGAACGGGGGGTTCGTGCATACAGTCCAGCTTGGAGCGAACTG

CCTACCCGGAACTGAGTGTCAGGCGTGGAATGAGACAAACGCGGCCATAA

CAGCGGAATGACACCGGTAAACCGAAAGGCAGGAACAGGAGAGCGCACGA

GGGAGCCGCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTT

CGCCACCACTGATTTGAGCGTCAGATTTCGTGATGCTTGTCAGGGGGGCG

GAGCCTATGGAAAAACGGCTTTGCCGCGGCCCTCTCACTTCCCTGTTAAG

TATCTTCCTGGCATCTTCCAGGAAATCTCCGCCCCGTTCGTAAGCCATTT

CCGCTCGCCGCAGTCGAACGACCGAGCGTAGCGAGTCAGTGAGCGAGGAA

GCGGAATATATCCTGTATCACATATTCTGCTGACGCACCGGTGCAGCCTT

TTTTCTCCTGCCACATGAAGCACTTCACTGACACCCTCATCAGTGCCAAC

ATAGTAAGCCAGTATACACTCCGCTAGCGCGCAAGCGGCCGCA

>pMZLK_VL

(SEQ ID NO: 31)

[Image Omitted]

GGTCAGACCAAGTTTACTCAT

ATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAG

GTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTT

TTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTT

GAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCA

CCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTT

TCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTC

TAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCT

ACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGA

TAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGG

CGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAG

CGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAG

CGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCA

GGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGG

TATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATT

TTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACG

CGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTC

TTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGA

GTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAG

TGAGCGAGGAAGCGGATGAGCGCCCAATACGCAAACCGCCTCTCCCCGCG

CGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAA

AGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCATTAGG

CACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGAATT

GTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGC

CAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGC

TCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGG

CGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGC

GTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGC

CTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTG

CGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATG

CCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCC

TGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACC

[Image Omitted]

CCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGA

GACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATG

GCTAAAATGAGAATATCACCGGAATTGAAAAAACTGATCGAAAAATACCG

CTGCGTAAAAGATACGGAAGGAATGTCTCCTGCTAAGGTATATAAGCTGG

TGGGAGAAAATGAAAACCTATATTTAAAAATGACGGACAGCCGGTATAAA

GGGACCACCTATGATGTGGAACGGGAAAAGGACATGATGCTATGGCTGGA

AGGAAAGCTGCCTGTTCCAAAGGTCCTGCACTTTGAACGGCATGATGGCT

GGAGCAATCTGCTCATGAGTGAGGCCGATGGCGTCCTTTGCTCGGAAGAG

TATGAAGATGAACAAAGCCCTGAAAAGATTATCGAGCTGTATGCGGAGTG

CATCAGGCTCTTTCACTCCATCGACATATCGGATTGTCCCTATACGAATA

GCTTAGACAGCCGCTTAGCCGAATTGGATTACTTACTGAATAACGATCTG

GCCGATGTGGATTGCGAAAACTGGGAAGAGGACACTCCATTTAAAGATCC

GCGCGAGCTGTATGATTTTTTAAAGACGGAAAAGCCCGAAGAGGAACTTG

TCTTTTCCCACGGCGACCTGGGAGACAGCAACATCTTTGTGAAAGATGGC

AAAGTAAGTGGCTTTATTGATCTTGGGAGAAGCGGCAGGGCGGACAAGTG

GTATGACATTGCCTTCTGCGTCCGGTCGCTCAGGGAGGATATCGGGGAAG

AACAGTATGTCGAGCTATTTTTTGACTTACTGGGGATCAAGCCTGATTGG

GAGAAAATAAAATATTATATTTTACTGGATGAATTGTTTTAGGAGCCTGT

TTAATAAGATGATCTTCTTGAGATCGTTTTGGTCTGCGCGTAATCTCTTG

CTCTGAAAACGAAAAAACCGCCTTGCAGGGCGGTTTTTCGAAGGTTCTCT

GAGCTACCAACTCTTTGAACCGAGGTAACTGGCTTGGAGGAGCGCAGTCA

CCAAAACTTGTCCTTTCAGTTTAGCCTTAACCGGCGCATGACTTCAAGAC

TAACTCCTCTAAATCAATTACCAGTGGCTGCTGCCAGTGGTGCTTTTGCA

TGTCTTTCCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCG

GTCGGACTGAACGGGGGGTTCGTGCATACAGTCCAGCTTGGAGCGAACTG

CCTACCCGGAACTGAGTGTCAGGCGTGGAATGAGACAAACGCGGCCATAA

CAGCGGAATGACACCGGTAAACCGAAAGGCAGGAACAGGAGAGCGCACGA

GGGAGCCGCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTT

CGCCACCACTGATTTGAGCGTCAGATTTCGTGATGCTTGTCAGGGGGGCG

GAGCCTATGGAAAAACGGCTTTGCCGCGGCCCTCTCACTTCCCTGTTAAG

TATCTTCCTGGCATCTTCCAGGAAATCTCCGCCCCGTTCGTAAGCCATTT

CCGCTCGCCGCAGTCGAACGACCGAGCGTAGCGAGTCAGTGAGCGAGGAA

GCGGAATATATCCTGTATCACATATTCTGCTGACGCACCGGTGCAGCCTT

TTTTCTCCTGCCACATGAAGCACTTCACTGACACCCTCATCAGTGCCAAC

ATAGTAAGCCAGTATACACTCCGCTAGCGCGCAAGCGGCCGCA

>pMZLK_VM

(SEQ ID NO: 32)

[Image Omitted]

GGTCAGACCAAGTTTACTCAT

ATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAG

GTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTT

TTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTT

GAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCA

CCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTT

TCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTC

TAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCT

ACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGA

TAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGG

CGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAG

CGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAG

CGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCA

GGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGG

TATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATT

TTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACG

CGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTC

TTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGA

GTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAG

TGAGCGAGGAAGCGGATGAGCGCCCAATACGCAAACCGCCTCTCCCCGCG

CGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAA

AGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCATTAGG

CACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGAATT

GTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGC

CAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGC

TCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGG

CGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGC

GTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGC

CTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTG

CGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATG

CCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCC

TGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACC

[Image Omitted]

CCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGA

GACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATG

GCTAAAATGAGAATATCACCGGAATTGAAAAAACTGATCGAAAAATACCG

CTGCGTAAAAGATACGGAAGGAATGTCTCCTGCTAAGGTATATAAGCTGG

TGGGAGAAAATGAAAACCTATATTTAAAAATGACGGACAGCCGGTATAAA

GGGACCACCTATGATGTGGAACGGGAAAAGGACATGATGCTATGGCTGGA

AGGAAAGCTGCCTGTTCCAAAGGTCCTGCACTTTGAACGGCATGATGGCT

GGAGCAATCTGCTCATGAGTGAGGCCGATGGCGTCCTTTGCTCGGAAGAG

TATGAAGATGAACAAAGCCCTGAAAAGATTATCGAGCTGTATGCGGAGTG

CATCAGGCTCTTTCACTCCATCGACATATCGGATTGTCCCTATACGAATA

GCTTAGACAGCCGCTTAGCCGAATTGGATTACTTACTGAATAACGATCTG

GCCGATGTGGATTGCGAAAACTGGGAAGAGGACACTCCATTTAAAGATCC

GCGCGAGCTGTATGATTTTTTAAAGACGGAAAAGCCCGAAGAGGAACTTG

TCTTTTCCCACGGCGACCTGGGAGACAGCAACATCTTTGTGAAAGATGGC

AAAGTAAGTGGCTTTATTGATCTTGGGAGAAGCGGCAGGGCGGACAAGTG

GTATGACATTGCCTTCTGCGTCCGGTCGCTCAGGGAGGATATCGGGGAAG

AACAGTATGTCGAGCTATTTTTTGACTTACTGGGGATCAAGCCTGATTGG

GAGAAAATAAAATATTATATTTTACTGGATGAATTGTTTTAGGAGCCTGT

TTAATAAGATGATCTTCTTGAGATCGTTTTGGTCTGCGCGTAATCTCTTG

CTCTGAAAACGAAAAAACCGCCTTGCAGGGCGGTTTTTCGAAGGTTCTCT

GAGCTACCAACTCTTTGAACCGAGGTAACTGGCTTGGAGGAGCGCAGTCA

CCAAAACTTGTCCTTTCAGTTTAGCCTTAACCGGCGCATGACTTCAAGAC

TAACTCCTCTAAATCAATTACCAGTGGCTGCTGCCAGTGGTGCTTTTGCA

TGTCTTTCCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCG

GTCGGACTGAACGGGGGGTTCGTGCATACAGTCCAGCTTGGAGCGAACTG

CCTACCCGGAACTGAGTGTCAGGCGTGGAATGAGACAAACGCGGCCATAA

CAGCGGAATGACACCGGTAAACCGAAAGGCAGGAACAGGAGAGCGCACGA

GGGAGCCGCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTT

CGCCACCACTGATTTGAGCGTCAGATTTCGTGATGCTTGTCAGGGGGGCG

GAGCCTATGGAAAAACGGCTTTGCCGCGGCCCTCTCACTTCCCTGTTAAG

TATCTTCCTGGCATCTTCCAGGAAATCTCCGCCCCGTTCGTAAGCCATTT

CCGCTCGCCGCAGTCGAACGACCGAGCGTAGCGAGTCAGTGAGCGAGGAA

GCGGAATATATCCTGTATCACATATTCTGCTGACGCACCGGTGCAGCCTT

TTTTCTCCTGCCACATGAAGCACTTCACTGACACCCTCATCAGTGCCAAC

ATAGTAAGCCAGTATACACTCCGCTAGCGCGCAAGCGGCCGCA

>pMZLK_VR

(SEQ ID NO: 33)

[Image Omitted]

GGTCAGACCAAGTTTACTCAT

ATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAG

GTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTT

TTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTT

GAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCA

CCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTT

TCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTC

TAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCT

ACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGA

TAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGG

CGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAG

CGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAG

CGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCA

GGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGG

TATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATT

TTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACG

CGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTC

TTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGA

GTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAG

TGAGCGAGGAAGCGGATGAGCGCCCAATACGCAAACCGCCTCTCCCCGCG

CGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAA

AGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCATTAGG

CACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGAATT

GTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGC

CAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGC

TCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGG

CGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGC

GTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGC

CTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTG

CGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATG

CCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCC

TGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACC

[Image Omitted]

CCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGA

GACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATG

GCTAAAATGAGAATATCACCGGAATTGAAAAAACTGATCGAAAAATACCG

CTGCGTAAAAGATACGGAAGGAATGTCTCCTGCTAAGGTATATAAGCTGG

TGGGAGAAAATGAAAACCTATATTTAAAAATGACGGACAGCCGGTATAAA

GGGACCACCTATGATGTGGAACGGGAAAAGGACATGATGCTATGGCTGGA

AGGAAAGCTGCCTGTTCCAAAGGTCCTGCACTTTGAACGGCATGATGGCT

GGAGCAATCTGCTCATGAGTGAGGCCGATGGCGTCCTTTGCTCGGAAGAG

TATGAAGATGAACAAAGCCCTGAAAAGATTATCGAGCTGTATGCGGAGTG

CATCAGGCTCTTTCACTCCATCGACATATCGGATTGTCCCTATACGAATA

GCTTAGACAGCCGCTTAGCCGAATTGGATTACTTACTGAATAACGATCTG

GCCGATGTGGATTGCGAAAACTGGGAAGAGGACACTCCATTTAAAGATCC

GCGCGAGCTGTATGATTTTTTAAAGACGGAAAAGCCCGAAGAGGAACTTG

TCTTTTCCCACGGCGACCTGGGAGACAGCAACATCTTTGTGAAAGATGGC

AAAGTAAGTGGCTTTATTGATCTTGGGAGAAGCGGCAGGGCGGACAAGTG

GTATGACATTGCCTTCTGCGTCCGGTCGCTCAGGGAGGATATCGGGGAAG

AACAGTATGTCGAGCTATTTTTTGACTTACTGGGGATCAAGCCTGATTGG

GAGAAAATAAAATATTATATTTTACTGGATGAATTGTTTTAGGAGCCTGT

TTAATAAGATGATCTTCTTGAGATCGTTTTGGTCTGCGCGTAATCTCTTG

CTCTGAAAACGAAAAAACCGCCTTGCAGGGCGGTTTTTCGAAGGTTCTCT

GAGCTACCAACTCTTTGAACCGAGGTAACTGGCTTGGAGGAGCGCAGTCA

CCAAAACTTGTCCTTTCAGTTTAGCCTTAACCGGCGCATGACTTCAAGAC

TAACTCCTCTAAATCAATTACCAGTGGCTGCTGCCAGTGGTGCTTTTGCA

TGTCTTTCCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCG

GTCGGACTGAACGGGGGGTTCGTGCATACAGTCCAGCTTGGAGCGAACTG

CCTACCCGGAACTGAGTGTCAGGCGTGGAATGAGACAAACGCGGCCATAA

CAGCGGAATGACACCGGTAAACCGAAAGGCAGGAACAGGAGAGCGCACGA

GGGAGCCGCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTT

CGCCACCACTGATTTGAGCGTCAGATTTCGTGATGCTTGTCAGGGGGGCG

GAGCCTATGGAAAAACGGCTTTGCCGCGGCCCTCTCACTTCCCTGTTAAG

TATCTTCCTGGCATCTTCCAGGAAATCTCCGCCCCGTTCGTAAGCCATTT

CCGCTCGCCGCAGTCGAACGACCGAGCGTAGCGAGTCAGTGAGCGAGGAA

GCGGAATATATCCTGTATCACATATTCTGCTGACGCACCGGTGCAGCCTT

TTTTCTCCTGCCACATGAAGCACTTCACTGACACCCTCATCAGTGCCAAC

ATAGTAAGCCAGTATACACTCCGCTAGCGCGCAAGCGGCCGCA

>pMXLS_VL

(SEQ ID NO: 34)

[Image Omitted]

GTCAGACCAAGTTTACTCAT

ATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAG

GTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTT

TTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTT

GAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCA

CCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTT

TCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTC

TAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCT

ACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGA

TAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGG

CGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAG

CGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAG

CGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCA

GGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGG

TATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATT

TTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACG

CGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTC

TTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGA

GTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAG

TGAGCGAGGAAGCGGATGAGCGCCCAATACGCAAACCGCCTCTCCCCGCG

CGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAA

AGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCATTAGG

CACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGAATT

GTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGC

CAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGC

TCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGG

CGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGC

GTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGC

CTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTG

CGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATG

CCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCC

TGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGAC

[Image Omitted]

TGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTAT

CCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAG

GAAGAGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCG

GTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTTT

GGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGT

GGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCA

GTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAA

GTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGA

ACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCC

TGAAGCCACACAGCGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTT

GATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGC

TTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTG

TGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAA

TTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGC

CACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATA

GCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCT

GAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTC

GCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCC

GCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCT

GCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACT

TGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGC

GCGCAGATCAGTTGGAAGAATTTGTCCATTACGTAAAAGGCGAGATCACC

AAGGTAGTCGGCAAATAAGAGCCTGTTTAATAAGATGATCTTCTTGAGAT

CGTTTTGGTCTGCGCGTAATCTCTTGCTCTGAAAACGAAAAAACCGCCTT

GCAGGGCGGTTTTTCGAAGGTTCTCTGAGCTACCAACTCTTTGAACCGAG

GTAACTGGCTTGGAGGAGCGCAGTCACCAAAACTTGTCCTTTCAGTTTAG

CCTTAACCGGCGCATGACTTCAAGACTAACTCCTCTAAATCAATTACCAG

TGGCTGCTGCCAGTGGTGCTTTTGCATGTCTTTCCGGGTTGGACTCAAGA

CGATAGTTACCGGATAAGGCGCAGCGGTCGGACTGAACGGGGGGTTCGTG

CATACAGTCCAGCTTGGAGCGAACTGCCTACCCGGAACTGAGTGTCAGGC

GTGGAATGAGACAAACGCGGCCATAACAGCGGAATGACACCGGTAAACCG

AAAGGCAGGAACAGGAGAGCGCACGAGGGAGCCGCCAGGGGGAAACGCCT

GGTATCTTTATAGTCCTGTCGGGTTTCGCCACCACTGATTTGAGCGTCAG

ATTTCGTGATGCTTGTCAGGGGGGCGGAGCCTATGGAAAAACGGCTTTGC

CGCGGCCCTCTCACTTCCCTGTTAAGTATCTTCCTGGCATCTTCCAGGAA

ATCTCCGCCCCGTTCGTAAGCCATTTCCGCTCGCCGCAGTCGAACGACCG

AGCGTAGCGAGTCAGTGAGCGAGGAAGCGGAATATATCCTGTATCACATA

TTCTGCTGACGCACCGGTGCAGCCTTTTTTCTCCTGCCACATGAAGCACT

TCACTGACACCCTCATCAGTGCCAACATAGTAAGCCAGTATACACTCCGC

TAGCGCGCAAGCGGCCGCA

>pMXLS_VM

(SEQ ID NO: 35)

[Image Omitted]

GTCAGACCAAGTTTACTCAT

ATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAG

GTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTT

TTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTT

GAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCA

CCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTT

TCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTC

TAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCT

ACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGA

TAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGG

CGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAG

CGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAG

CGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCA

GGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGG

TATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATT

TTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACG

CGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTC

TTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGA

GTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAG

TGAGCGAGGAAGCGGATGAGCGCCCAATACGCAAACCGCCTCTCCCCGCG

CGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAA

AGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCATTAGG

CACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGAATT

GTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGC

CAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGC

TCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGG

CGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGC

GTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGC

CTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTG

CGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATG

CCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCC

TGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGAC

[Image Omitted]

TGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTAT

CCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAG

GAAGAGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCG

GTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTTT

GGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGT

GGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCA

GTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAA

GTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGA

ACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCC

TGAAGCCACACAGCGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTT

GATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGC

TTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTG

TGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAA

TTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGC

CACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATA

GCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCT

GAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTC

GCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCC

GCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCT

GCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACT

TGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGC

GCGCAGATCAGTTGGAAGAATTTGTCCATTACGTAAAAGGCGAGATCACC

AAGGTAGTCGGCAAATAAGAGCCTGTTTAATAAGATGATCTTCTTGAGAT

CGTTTTGGTCTGCGCGTAATCTCTTGCTCTGAAAACGAAAAAACCGCCTT

GCAGGGCGGTTTTTCGAAGGTTCTCTGAGCTACCAACTCTTTGAACCGAG

GTAACTGGCTTGGAGGAGCGCAGTCACCAAAACTTGTCCTTTCAGTTTAG

CCTTAACCGGCGCATGACTTCAAGACTAACTCCTCTAAATCAATTACCAG

TGGCTGCTGCCAGTGGTGCTTTTGCATGTCTTTCCGGGTTGGACTCAAGA

CGATAGTTACCGGATAAGGCGCAGCGGTCGGACTGAACGGGGGGTTCGTG

CATACAGTCCAGCTTGGAGCGAACTGCCTACCCGGAACTGAGTGTCAGGC

GTGGAATGAGACAAACGCGGCCATAACAGCGGAATGACACCGGTAAACCG

AAAGGCAGGAACAGGAGAGCGCACGAGGGAGCCGCCAGGGGGAAACGCCT

GGTATCTTTATAGTCCTGTCGGGTTTCGCCACCACTGATTTGAGCGTCAG

ATTTCGTGATGCTTGTCAGGGGGGCGGAGCCTATGGAAAAACGGCTTTGC

CGCGGCCCTCTCACTTCCCTGTTAAGTATCTTCCTGGCATCTTCCAGGAA

ATCTCCGCCCCGTTCGTAAGCCATTTCCGCTCGCCGCAGTCGAACGACCG

AGCGTAGCGAGTCAGTGAGCGAGGAAGCGGAATATATCCTGTATCACATA

TTCTGCTGACGCACCGGTGCAGCCTTTTTTCTCCTGCCACATGAAGCACT

TCACTGACACCCTCATCAGTGCCAACATAGTAAGCCAGTATACACTCCGC

TAGCGCGCAAGCGGCCGCA

>pMXLS_VR

(SEQ ID NO: 36)

[Image Omitted]

GTCAGACCAAGTTTACTCAT

ATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAG

GTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTT

TTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTT

GAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCA

CCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTT

TCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTC

TAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCT

ACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGA

TAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGG

CGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAG

CGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAG

CGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCA

GGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGG

TATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATT

TTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACG

CGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTC

TTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGA

GTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAG

TGAGCGAGGAAGCGGATGAGCGCCCAATACGCAAACCGCCTCTCCCCGCG

CGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAA

AGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCATTAGG

CACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGAATT

GTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGC

CAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGC

TCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGG

CGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGC

GTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGC

CTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTG

CGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATG

CCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCC

TGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGAC

[Image Omitted]

TGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTAT

CCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAG

GAAGAGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCG

GTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTTT

GGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGT

GGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCA

GTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAA

GTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGA

ACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCC

TGAAGCCACACAGCGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTT

GATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGC

TTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTG

TGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAA

TTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGC

CACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATA

GCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCT

GAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTC

GCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCC

GCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCT

GCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACT

TGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGC

GCGCAGATCAGTTGGAAGAATTTGTCCATTACGTAAAAGGCGAGATCACC

AAGGTAGTCGGCAAATAAGAGCCTGTTTAATAAGATGATCTTCTTGAGAT

CGTTTTGGTCTGCGCGTAATCTCTTGCTCTGAAAACGAAAAAACCGCCTT

GCAGGGCGGTTTTTCGAAGGTTCTCTGAGCTACCAACTCTTTGAACCGAG

GTAACTGGCTTGGAGGAGCGCAGTCACCAAAACTTGTCCTTTCAGTTTAG

CCTTAACCGGCGCATGACTTCAAGACTAACTCCTCTAAATCAATTACCAG

TGGCTGCTGCCAGTGGTGCTTTTGCATGTCTTTCCGGGTTGGACTCAAGA

CGATAGTTACCGGATAAGGCGCAGCGGTCGGACTGAACGGGGGGTTCGTG

CATACAGTCCAGCTTGGAGCGAACTGCCTACCCGGAACTGAGTGTCAGGC

GTGGAATGAGACAAACGCGGCCATAACAGCGGAATGACACCGGTAAACCG

AAAGGCAGGAACAGGAGAGCGCACGAGGGAGCCGCCAGGGGGAAACGCCT

GGTATCTTTATAGTCCTGTCGGGTTTCGCCACCACTGATTTGAGCGTCAG

ATTTCGTGATGCTTGTCAGGGGGGCGGAGCCTATGGAAAAACGGCTTTGC

CGCGGCCCTCTCACTTCCCTGTTAAGTATCTTCCTGGCATCTTCCAGGAA

ATCTCCGCCCCGTTCGTAAGCCATTTCCGCTCGCCGCAGTCGAACGACCG

AGCGTAGCGAGTCAGTGAGCGAGGAAGCGGAATATATCCTGTATCACATA

TTCTGCTGACGCACCGGTGCAGCCTTTTTTCTCCTGCCACATGAAGCACT

TCACTGACACCCTCATCAGTGCCAACATAGTAAGCCAGTATACACTCCGC

TAGCGCGCAAGCGGCCGCA

>pMZLS_VL

(SEQ ID NO: 37)

[Image Omitted]

GGTCAGACCAAGTTTACTCAT

ATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAG

GTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTT

TTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTT

GAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCA

CCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTT

TCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTC

TAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCT

ACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGA

TAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGG

CGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAG

CGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAG

CGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCA

GGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGG

TATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATT

TTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACG

CGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTC

TTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGA

GTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAG

TGAGCGAGGAAGCGGATGAGCGCCCAATACGCAAACCGCCTCTCCCCGCG

CGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAA

AGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCATTAGG

CACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGAATT

GTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGC

CAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGC

TCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGG

CGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGC

GTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGC

CTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTG

CGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATG

CCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCC

TGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACC

[Image Omitted]

TGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTAT

CCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAG

GAAGAGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCG

GTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTTT

GGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGT

GGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCA

GTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAA

GTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGA

ACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCC

TGAAGCCACACAGCGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTT

GATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGC

TTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTG

TGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAA

TTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGC

CACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATA

GCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCT

GAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTC

GCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCC

GCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCT

GCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACT

TGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGC

GCGCAGATCAGTTGGAAGAATTTGTCCATTACGTAAAAGGCGAGATCACC

AAGGTAGTCGGCAAATAAGAGCCTGTTTAATAAGATGATCTTCTTGAGAT

CGTTTTGGTCTGCGCGTAATCTCTTGCTCTGAAAACGAAAAAACCGCCTT

GCAGGGCGGTTTTTCGAAGGTTCTCTGAGCTACCAACTCTTTGAACCGAG

GTAACTGGCTTGGAGGAGCGCAGTCACCAAAACTTGTCCTTTCAGTTTAG

CCTTAACCGGCGCATGACTTCAAGACTAACTCCTCTAAATCAATTACCAG

TGGCTGCTGCCAGTGGTGCTTTTGCATGTCTTTCCGGGTTGGACTCAAGA

CGATAGTTACCGGATAAGGCGCAGCGGTCGGACTGAACGGGGGGTTCGTG

CATACAGTCCAGCTTGGAGCGAACTGCCTACCCGGAACTGAGTGTCAGGC

GTGGAATGAGACAAACGCGGCCATAACAGCGGAATGACACCGGTAAACCG

AAAGGCAGGAACAGGAGAGCGCACGAGGGAGCCGCCAGGGGGAAACGCCT

GGTATCTTTATAGTCCTGTCGGGTTTCGCCACCACTGATTTGAGCGTCAG

ATTTCGTGATGCTTGTCAGGGGGGCGGAGCCTATGGAAAAACGGCTTTGC

CGCGGCCCTCTCACTTCCCTGTTAAGTATCTTCCTGGCATCTTCCAGGAA

ATCTCCGCCCCGTTCGTAAGCCATTTCCGCTCGCCGCAGTCGAACGACCG

AGCGTAGCGAGTCAGTGAGCGAGGAAGCGGAATATATCCTGTATCACATA

TTCTGCTGACGCACCGGTGCAGCCTTTTTTCTCCTGCCACATGAAGCACT

TCACTGACACCCTCATCAGTGCCAACATAGTAAGCCAGTATACACTCCGC

TAGCGCGCAAGCGGCCGCA

>pMZLS_VM

(SEQ ID NO: 38)

[Image Omitted]

GGTCAGACCAAGTTTACTCAT

ATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAG

GTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTT

TTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTT

GAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCA

CCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTT

TCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTC

TAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCT

ACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGA

TAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGG

CGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAG

CGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAG

CGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCA

GGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGG

TATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATT

TTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACG

CGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTC

TTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGA

GTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAG

TGAGCGAGGAAGCGGATGAGCGCCCAATACGCAAACCGCCTCTCCCCGCG

CGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAA

AGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCATTAGG

CACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGAATT

GTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGC

CAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGC

TCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGG

CGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGC

GTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGC

CTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTG

CGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATG

CCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCC

TGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACC

[Image Omitted]

TGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTAT

CCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAG

GAAGAGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCG

GTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTTT

GGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGT

GGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCA

GTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAA

GTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGA

ACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCC

TGAAGCCACACAGCGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTT

GATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGC

TTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTG

TGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAA

TTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGC

CACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATA

GCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCT

GAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTC

GCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCC

GCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCT

GCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACT

TGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGC

GCGCAGATCAGTTGGAAGAATTTGTCCATTACGTAAAAGGCGAGATCACC

AAGGTAGTCGGCAAATAAGAGCCTGTTTAATAAGATGATCTTCTTGAGAT

CGTTTTGGTCTGCGCGTAATCTCTTGCTCTGAAAACGAAAAAACCGCCTT

GCAGGGCGGTTTTTCGAAGGTTCTCTGAGCTACCAACTCTTTGAACCGAG

GTAACTGGCTTGGAGGAGCGCAGTCACCAAAACTTGTCCTTTCAGTTTAG

CCTTAACCGGCGCATGACTTCAAGACTAACTCCTCTAAATCAATTACCAG

TGGCTGCTGCCAGTGGTGCTTTTGCATGTCTTTCCGGGTTGGACTCAAGA

CGATAGTTACCGGATAAGGCGCAGCGGTCGGACTGAACGGGGGGTTCGTG

CATACAGTCCAGCTTGGAGCGAACTGCCTACCCGGAACTGAGTGTCAGGC

GTGGAATGAGACAAACGCGGCCATAACAGCGGAATGACACCGGTAAACCG

AAAGGCAGGAACAGGAGAGCGCACGAGGGAGCCGCCAGGGGGAAACGCCT

GGTATCTTTATAGTCCTGTCGGGTTTCGCCACCACTGATTTGAGCGTCAG

ATTTCGTGATGCTTGTCAGGGGGGCGGAGCCTATGGAAAAACGGCTTTGC

CGCGGCCCTCTCACTTCCCTGTTAAGTATCTTCCTGGCATCTTCCAGGAA

ATCTCCGCCCCGTTCGTAAGCCATTTCCGCTCGCCGCAGTCGAACGACCG

AGCGTAGCGAGTCAGTGAGCGAGGAAGCGGAATATATCCTGTATCACATA

TTCTGCTGACGCACCGGTGCAGCCTTTTTTCTCCTGCCACATGAAGCACT

TCACTGACACCCTCATCAGTGCCAACATAGTAAGCCAGTATACACTCCGC

TAGCGCGCAAGCGGCCGCA

>pMZLS_VR

(SEQ ID NO: 39)

[Image Omitted]

GGTCAGACCAAGTTTACTCAT

ATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAG

GTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTT

TTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTT

GAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCA

CCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTT

TCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTC

TAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCT

ACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGA

TAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGG

CGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAG

CGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAG

CGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCA

GGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGG

TATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATT

TTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACG

CGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTC

TTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGA

GTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAG

TGAGCGAGGAAGCGGATGAGCGCCCAATACGCAAACCGCCTCTCCCCGCG

CGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAA

AGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCATTAGG

CACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGAATT

GTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGC

CAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGC

TCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGG

CGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGC

GTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGC

CTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTG

CGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATG

CCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCC

TGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACC

[Image Omitted]

TGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTAT

CCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAG

GAAGAGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCG

GTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTTT

GGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGT

GGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCA

GTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAA

GTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGA

ACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCC

TGAAGCCACACAGCGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTT

GATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGC

TTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTG

TGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAA

TTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGC

CACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATA

GCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCT

GAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTC

GCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCC

GCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCT

GCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACT

TGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGC

GCGCAGATCAGTTGGAAGAATTTGTCCATTACGTAAAAGGCGAGATCACC

AAGGTAGTCGGCAAATAAGAGCCTGTTTAATAAGATGATCTTCTTGAGAT

CGTTTTGGTCTGCGCGTAATCTCTTGCTCTGAAAACGAAAAAACCGCCTT

GCAGGGCGGTTTTTCGAAGGTTCTCTGAGCTACCAACTCTTTGAACCGAG

GTAACTGGCTTGGAGGAGCGCAGTCACCAAAACTTGTCCTTTCAGTTTAG

CCTTAACCGGCGCATGACTTCAAGACTAACTCCTCTAAATCAATTACCAG

TGGCTGCTGCCAGTGGTGCTTTTGCATGTCTTTCCGGGTTGGACTCAAGA

CGATAGTTACCGGATAAGGCGCAGCGGTCGGACTGAACGGGGGGTTCGTG

CATACAGTCCAGCTTGGAGCGAACTGCCTACCCGGAACTGAGTGTCAGGC

GTGGAATGAGACAAACGCGGCCATAACAGCGGAATGACACCGGTAAACCG

AAAGGCAGGAACAGGAGAGCGCACGAGGGAGCCGCCAGGGGGAAACGCCT

GGTATCTTTATAGTCCTGTCGGGTTTCGCCACCACTGATTTGAGCGTCAG

ATTTCGTGATGCTTGTCAGGGGGGCGGAGCCTATGGAAAAACGGCTTTGC

CGCGGCCCTCTCACTTCCCTGTTAAGTATCTTCCTGGCATCTTCCAGGAA

ATCTCCGCCCCGTTCGTAAGCCATTTCCGCTCGCCGCAGTCGAACGACCG

AGCGTAGCGAGTCAGTGAGCGAGGAAGCGGAATATATCCTGTATCACATA

TTCTGCTGACGCACCGGTGCAGCCTTTTTTCTCCTGCCACATGAAGCACT

TCACTGACACCCTCATCAGTGCCAACATAGTAAGCCAGTATACACTCCGC

TAGCGCGCAAGCGGCCGCA

>pMXP_A4E

(SEQ ID NO: 40)

[Image Omitted]

TTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTA

GCTCACTCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTA

TGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTAT

GACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATC

CCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGAC

TGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCC

TTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCC

AACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTC

CTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTAC

AATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACAC

CCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGA

[Image Omitted]

ACTATCAGTGTTTGACAGGATATATTGGCGGGTAAACCTAAGAGAAAAGA

GCGTTTATTAGAATAATCGGATATTTAAAAGGGCGTGAAAAGGTTTATCC

GTTCGTCCATTTGTATGTGCATGCCAACCACAGGGTTCCCCAGATCAGGC

GCTGGCTGCTGAACCCCCAGCCGGAACTGACCCCACAAGGCCCTAGCGTT

TGCAATGCACCAGGTCATCATTGACCCAGGCGTGTTCCACCAGGCCGCTG

CCTCGCAACTCTTCGCAGGCTTCGCCGACCTGCTCGCGCCACTTCTTCAC

GCGGGTGGAATCCGATCCGCACATGAGGCGGAAGGTTTCCAGCTTGAGCG

GGTACGGCTCCCGGTGCGAGCTGAAATAGTCGAACATCCGTCGGGCCGTC

GGCGACAGCTTGCGGTACTTCTCCCATATGAATTTCGTGTAGTGGTCGCC

AGCAAACAGCACGACGATTTCCTCGTCGATCAGGACCTGGCAACGGGACG

TTTTCTTGCCACGGTCCAGGACGCGGAAGCGGTGCAGCAGCGACACCGAT

TCCAGGTGCCCAACGCGGTCGGACGTGAAGCCCATCGCCGTCGCCTGTAG

GCGCGACAGGCATTCCTCGGCCTTCGTGTAATACCGGCCATTGATCGACC

AGCCCAGGTCCTGGCAAAGCTCGTAGAACGTGAAGGTGATCGGCTCGCCG

ATAGGGGTGCGCTTCGCGTACTCCAACACCTGCTGCCACACCAGTTCGTC

ATCGTCGGCCCGCAGCTCGACGCCGGTGTAGGTGATCTTCACGTCCTTGT

TGACGTGGAAAATGACCTTGTTTTGCAGCGCCTCGCGCGGGATTTTCTTG

TTGCGCGTGGTGAACAGGGCAGAGCGGGCCGTGTCGTTTGGCATCGCTCG

CATCGTGTCCGGCCACGGCGCAATATCGAACAAGGAAAGCTGCATTTCCT

TGATCTGCTGCTTCGTGTGTTTCAGCAACGCGGCCTGCTTGGCCTCGCTG

ACCTGTTTTGCCAGGTCCTCGCCGGCGGTTTTTCGCTTCTTGGTCGTCAT

AGTTCCTCGCGTGTCGATGGTCATCGACTTCGCCAAACCTGCCGCCTCCT

GTTCAAGACGACGCGAACGCTCCACGGCGGCCGATGGCGCGGGCAGGGCA

GGGGGAGCCAGTTGCACGCTGTCGCGCTCGATCTTGGCCGTAGCTTGCTG

GACCATCGAGCCGACGGACTGGAAGGTTTCGCGGGGCGCACGCATGACGG

TGCGGCTTGCGATGGTTTCGGCATCCTCGGCGGAAAACCCCGCGTCGATC

AGTTCTTGCCTGTATGCCTTCCGGTCAAACGTCCGATTCATTCACCCTCC

TTGCGGGATTGCCCCGACTCACGCCGGGGCAATGTGCCCTTATTCCTGAT

TTGACCCGCCTGGTGCCTTGGTGTCCAGATAATCCACCTTATCGGCAATG

AAGTCGGTCCCGTAGACCGTCTGGCCGTCCTTCTCGTACTTGGTATTCCG

AATCTTGCCCTGCACGAATACCAGCGACCCCTTGCCCAAATACTTGCCGT

GGGCCTCGGCCTGAGAGCCAAAACACTTGATGCGGAAGAAGTCGGTGCGC

TCCTGCTTGTCGCCGGCATCGTTGCGCCACATCTAGGATCTGCCAGGAAC

CGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGA

CGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAG

GACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCT

CCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTC

GGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGG

TGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAG

CCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGT

AAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCA

GAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAAC

TACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCC

AGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCA

CCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGA

AAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGC

TCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAA

AAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCA

ATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAAT

CAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTG

CCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCT

GGCCCCAGTGCTGCAATGATACCGCGAGAACCACGCTCACCGGCTCCAGA

TTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTC

CTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCT

AGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGC

TACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCT

CCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAA

AAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGC

CGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTG

TCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAG

TCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTC

AATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCA

TTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTG

AGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATC

TTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATG

CCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTC

TTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAG

CGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGC

GCACGAATTGGCCAGCGCTGCCATTTTTGGGGTGAGGCCGTTCGCGGCCG

AGGGGCGCAGCCCCTGGGGGGATGGGAGGCCCGCGTTAGCGGGCCGGGAG

GGTTCGAGAAGGGGGGGCACCCCCCTTCGGCGTGCGCGGTCACGCGCACA

GGGCGCAGCCCTGGTTAAAAACAAGGTTTATAAATATTGGTTTAAAAGCA

GGTTAAAAGACAGGTTAGCGGTGGCCGAAAAACGGGCGGAAACCCTTGCA

AATGCTGGATTTTCTGCCTGTGGACAGCCCCTCAAATGTCAATAGGTGCG

CCCCTCATCTGTCAGCACTCTGCCCCTCAAGTGTCAAGGATCGCGCCCCT

CATCTGTCAGTAGTCGCGCCCCTCAAGTGTCAATACCGCAGGGCACTTAT

CCCCAGGCTTGTCCACATCATCTGTGGGAAACTCGCGTAAAATCAGGCGT

TTTCGCCGATTTGCGAGGCTGGCCAGCTCCACGTCGCCGGCCGAAATCGA

GCCTGCCCCTCATCTGTCAACGCCGCGCCGGGTGAGTCGGCCCCTCAAGT

GTCAACGTCCGCCCCTCATCTGTCAGTGAGGGCCAAGTTTTCCGCGAGGT

ATCCACAACGCCGGCGGCCGCGGTGTCTCGCACACGGCTTCGACGGCGTT

TCTGGCGCGTTTGCAGGGCCATAGACGGCCGCCAGCCCAGCGGCGAGGGC

AACCAGCCCGGTGAGCGTCGCAAAGGAGATCCTGATCTGACTGATGGGCT

GCCTGTATCGAGTGGTGATTTTGTGCCGAGCTGCCGGTCGGGGAGCTGTT

GGCTGGCTGGTGGCAGGATATATTGTGGTGTAAACAAATTGACGCTTAGA

CAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGATGCAG

TGGGCCC

>pMYP_A5E

(SEQ ID NO: 41)

[Image Omitted]

CCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCT

CACTCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGT

TGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGAC

CATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCC

GGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGG

GAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTT

CGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAAC

AGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTT

ACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAAT

CTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCG

CTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAA

[Image Omitted]

AGTGTTTGACAGGATATATTGGCGGGTAAACCTAAGAGAAAAGAGCGTTT

ATTAGAATAATCGGATATTTAAAAGGGCGTGAAAAGGTTTATCCGTTCGT

CCATTTGTATGTGCATGCCAACCACAGGGTTCCCCAGATCAGGCGCTGGC

TGCTGAACCCCCAGCCGGAACTGACCCCACAAGGCCCTAGCGTTTGCAAT

GCACCAGGTCATCATTGACCCAGGCGTGTTCCACCAGGCCGCTGCCTCGC

AACTCTTCGCAGGCTTCGCCGACCTGCTCGCGCCACTTCTTCACGCGGGT

GGAATCCGATCCGCACATGAGGCGGAAGGTTTCCAGCTTGAGCGGGTACG

GCTCCCGGTGCGAGCTGAAATAGTCGAACATCCGTCGGGCCGTCGGCGAC

AGCTTGCGGTACTTCTCCCATATGAATTTCGTGTAGTGGTCGCCAGCAAA

CAGCACGACGATTTCCTCGTCGATCAGGACCTGGCAACGGGACGTTTTCT

TGCCACGGTCCAGGACGCGGAAGCGGTGCAGCAGCGACACCGATTCCAGG

TGCCCAACGCGGTCGGACGTGAAGCCCATCGCCGTCGCCTGTAGGCGCGA

CAGGCATTCCTCGGCCTTCGTGTAATACCGGCCATTGATCGACCAGCCCA

GGTCCTGGCAAAGCTCGTAGAACGTGAAGGTGATCGGCTCGCCGATAGGG

GTGCGCTTCGCGTACTCCAACACCTGCTGCCACACCAGTTCGTCATCGTC

GGCCCGCAGCTCGACGCCGGTGTAGGTGATCTTCACGTCCTTGTTGACGT

GGAAAATGACCTTGTTTTGCAGCGCCTCGCGCGGGATTTTCTTGTTGCGC

GTGGTGAACAGGGCAGAGCGGGCCGTGTCGTTTGGCATCGCTCGCATCGT

GTCCGGCCACGGCGCAATATCGAACAAGGAAAGCTGCATTTCCTTGATCT

GCTGCTTCGTGTGTTTCAGCAACGCGGCCTGCTTGGCCTCGCTGACCTGT

TTTGCCAGGTCCTCGCCGGCGGTTTTTCGCTTCTTGGTCGTCATAGTTCC

TCGCGTGTCGATGGTCATCGACTTCGCCAAACCTGCCGCCTCCTGTTCAA

GACGACGCGAACGCTCCACGGCGGCCGATGGCGCGGGCAGGGCAGGGGGA

GCCAGTTGCACGCTGTCGCGCTCGATCTTGGCCGTAGCTTGCTGGACCAT

CGAGCCGACGGACTGGAAGGTTTCGCGGGGCGCACGCATGACGGTGCGGC

TTGCGATGGTTTCGGCATCCTCGGCGGAAAACCCCGCGTCGATCAGTTCT

TGCCTGTATGCCTTCCGGTCAAACGTCCGATTCATTCACCCTCCTTGCGG

GATTGCCCCGACTCACGCCGGGGCAATGTGCCCTTATTCCTGATTTGACC

CGCCTGGTGCCTTGGTGTCCAGATAATCCACCTTATCGGCAATGAAGTCG

GTCCCGTAGACCGTCTGGCCGTCCTTCTCGTACTTGGTATTCCGAATCTT

GCCCTGCACGAATACCAGCGACCCCTTGCCCAAATACTTGCCGTGGGCCT

CGGCCTGAGAGCCAAAACACTTGATGCGGAAGAAGTCGGTGCGCTCCTGC

TTGTCGCCGGCATCGTTGCGCCACATCTAGGATCTGCCAGGAACCGTAAA

AAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCA

TCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTAT

AAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTT

CCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAG

CGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGG

TCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGAC

CGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACA

CGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGA

GGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGC

TACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTAC

CTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTG

GTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAA

GGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTG

GAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGA

TCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAA

AGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGA

GGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGAC

TCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCC

AGTGCTGCAATGATACCGCGAGAACCACGCTCACCGGCTCCAGATTTATC

AGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAA

CTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTA

AGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGG

CATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTT

CCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCG

GTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGT

GTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGC

CATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTC

TGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACG

GGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAA

AACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCC

AGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTAC

TTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAA

AAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTT

TTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATA

CATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACGA

ATTGGCCAGCGCTGCCATTTTTGGGGTGAGGCCGTTCGCGGCCGAGGGGC

GCAGCCCCTGGGGGGATGGGAGGCCCGCGTTAGCGGGCCGGGAGGGTTCG

AGAAGGGGGGGCACCCCCCTTCGGCGTGCGCGGTCACGCGCACAGGGCGC

AGCCCTGGTTAAAAACAAGGTTTATAAATATTGGTTTAAAAGCAGGTTAA

AAGACAGGTTAGCGGTGGCCGAAAAACGGGCGGAAACCCTTGCAAATGCT

GGATTTTCTGCCTGTGGACAGCCCCTCAAATGTCAATAGGTGCGCCCCTC

ATCTGTCAGCACTCTGCCCCTCAAGTGTCAAGGATCGCGCCCCTCATCTG

TCAGTAGTCGCGCCCCTCAAGTGTCAATACCGCAGGGCACTTATCCCCAG

GCTTGTCCACATCATCTGTGGGAAACTCGCGTAAAATCAGGCGTTTTCGC

CGATTTGCGAGGCTGGCCAGCTCCACGTCGCCGGCCGAAATCGAGCCTGC

CCCTCATCTGTCAACGCCGCGCCGGGTGAGTCGGCCCCTCAAGTGTCAAC

GTCCGCCCCTCATCTGTCAGTGAGGGCCAAGTTTTCCGCGAGGTATCCAC

AACGCCGGCGGCCGCGGTGTCTCGCACACGGCTTCGACGGCGTTTCTGGC

GCGTTTGCAGGGCCATAGACGGCCGCCAGCCCAGCGGCGAGGGCAACCAG

CCCGGTGAGCGTCGCAAAGGAGATCCTGATCTGACTGATGGGCTGCCTGT

ATCGAGTGGTGATTTTGTGCCGAGCTGCCGGTCGGGGAGCTGTTGGCTGG

CTGGTGGCAGGATATATTGTGGTGTAAACAAATTGACGCTTAGACAACTT

AATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGATGCAGTGGGCC

C

>pMZP_P6T

(SEQ ID NO: 42)

[Image Omitted]

TTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAG

CTCACTCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTAT

GTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATG

ACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCC

CCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACT

GGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCT

TTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCA

ACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCC

TTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACA

ATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACC

CGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGAC

[Image Omitted]

TATCAGTGTTTGACAGGATATATTGGCGGGTAAACCTAAGAGAAAAGAGC

GTTTATTAGAATAATCGGATATTTAAAAGGGCGTGAAAAGGTTTATCCGT

TCGTCCATTTGTATGTGCATGCCAACCACAGGGTTCCCCAGATCAGGCGC

TGGCTGCTGAACCCCCAGCCGGAACTGACCCCACAAGGCCCTAGCGTTTG

CAATGCACCAGGTCATCATTGACCCAGGCGTGTTCCACCAGGCCGCTGCC

TCGCAACTCTTCGCAGGCTTCGCCGACCTGCTCGCGCCACTTCTTCACGC

GGGTGGAATCCGATCCGCACATGAGGCGGAAGGTTTCCAGCTTGAGCGGG

TACGGCTCCCGGTGCGAGCTGAAATAGTCGAACATCCGTCGGGCCGTCGG

CGACAGCTTGCGGTACTTCTCCCATATGAATTTCGTGTAGTGGTCGCCAG

CAAACAGCACGACGATTTCCTCGTCGATCAGGACCTGGCAACGGGACGTT

TTCTTGCCACGGTCCAGGACGCGGAAGCGGTGCAGCAGCGACACCGATTC

CAGGTGCCCAACGCGGTCGGACGTGAAGCCCATCGCCGTCGCCTGTAGGC

GCGACAGGCATTCCTCGGCCTTCGTGTAATACCGGCCATTGATCGACCAG

CCCAGGTCCTGGCAAAGCTCGTAGAACGTGAAGGTGATCGGCTCGCCGAT

AGGGGTGCGCTTCGCGTACTCCAACACCTGCTGCCACACCAGTTCGTCAT

CGTCGGCCCGCAGCTCGACGCCGGTGTAGGTGATCTTCACGTCCTTGTTG

ACGTGGAAAATGACCTTGTTTTGCAGCGCCTCGCGCGGGATTTTCTTGTT

GCGCGTGGTGAACAGGGCAGAGCGGGCCGTGTCGTTTGGCATCGCTCGCA

TCGTGTCCGGCCACGGCGCAATATCGAACAAGGAAAGCTGCATTTCCTTG

ATCTGCTGCTTCGTGTGTTTCAGCAACGCGGCCTGCTTGGCCTCGCTGAC

CTGTTTTGCCAGGTCCTCGCCGGCGGTTTTTCGCTTCTTGGTCGTCATAG

TTCCTCGCGTGTCGATGGTCATCGACTTCGCCAAACCTGCCGCCTCCTGT

TCAAGACGACGCGAACGCTCCACGGCGGCCGATGGCGCGGGCAGGGCAGG

GGGAGCCAGTTGCACGCTGTCGCGCTCGATCTTGGCCGTAGCTTGCTGGA

CCATCGAGCCGACGGACTGGAAGGTTTCGCGGGGCGCACGCATGACGGTG

CGGCTTGCGATGGTTTCGGCATCCTCGGCGGAAAACCCCGCGTCGATCAG

TTCTTGCCTGTATGCCTTCCGGTCAAACGTCCGATTCATTCACCCTCCTT

GCGGGATTGCCCCGACTCACGCCGGGGCAATGTGCCCTTATTCCTGATTT

GACCCGCCTGGTGCCTTGGTGTCCAGATAATCCACCTTATCGGCAATGAA

GTCGGTCCCGTAGACCGTCTGGCCGTCCTTCTCGTACTTGGTATTCCGAA

TCTTGCCCTGCACGAATACCAGCGACCCCTTGCCCAAATACTTGCCGTGG

GCCTCGGCCTGAGAGCCAAAACACTTGATGCGGAAGAAGTCGGTGCGCTC

CTGCTTGTCGCCGGCATCGTTGCGCCACATCTAGGATCTGCCAGGAACCG

TAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACG

AGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGA

CTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCC

TGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGG

GAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTG

TAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCC

CGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA

GACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGA

GCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTA

CGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAG

TTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACC

GCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAA

AAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTC

AGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAA

AGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAAT

CTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCA

GTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCC

TGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGG

CCCCAGTGCTGCAATGATACCGCGAGAACCACGCTCACCGGCTCCAGATT

TATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCT

GCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAG

AGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTA

CAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCC

GGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAA

AGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCG

CAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTC

ATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTC

ATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAA

TACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATT

GGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAG

ATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTT

TTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCC

GCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTT

CCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCG

GATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGC

ACGAATTGGCCAGCGCTGCCATTTTTGGGGTGAGGCCGTTCGCGGCCGAG

GGGCGCAGCCCCTGGGGGGATGGGAGGCCCGCGTTAGCGGGCCGGGAGGG

TTCGAGAAGGGGGGGCACCCCCCTTCGGCGTGCGCGGTCACGCGCACAGG

GCGCAGCCCTGGTTAAAAACAAGGTTTATAAATATTGGTTTAAAAGCAGG

TTAAAAGACAGGTTAGCGGTGGCCGAAAAACGGGCGGAAACCCTTGCAAA

TGCTGGATTTTCTGCCTGTGGACAGCCCCTCAAATGTCAATAGGTGCGCC

CCTCATCTGTCAGCACTCTGCCCCTCAAGTGTCAAGGATCGCGCCCCTCA

TCTGTCAGTAGTCGCGCCCCTCAAGTGTCAATACCGCAGGGCACTTATCC

CCAGGCTTGTCCACATCATCTGTGGGAAACTCGCGTAAAATCAGGCGTTT

TCGCCGATTTGCGAGGCTGGCCAGCTCCACGTCGCCGGCCGAAATCGAGC

CTGCCCCTCATCTGTCAACGCCGCGCCGGGTGAGTCGGCCCCTCAAGTGT

CAACGTCCGCCCCTCATCTGTCAGTGAGGGCCAAGTTTTCCGCGAGGTAT

CCACAACGCCGGCGGCCGCGGTGTCTCGCACACGGCTTCGACGGCGTTTC

TGGCGCGTTTGCAGGGCCATAGACGGCCGCCAGCCCAGCGGCGAGGGCAA

CCAGCCCGGTGAGCGTCGCAAAGGAGATCCTGATCTGACTGATGGGCTGC

CTGTATCGAGTGGTGATTTTGTGCCGAGCTGCCGGTCGGGGAGCTGTTGG

CTGGCTGGTGGCAGGATATATTGTGGTGTAAACAAATTGACGCTTAGACA

ACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGATGCAGTG

GGCCC

>pMXBG_A7I

(SEQ ID NO: 43)

[Image Omitted]

CATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATC

TAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGA

GTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTT

CTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAA

CCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCT

TTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCC

TTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCG

CCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGG

CGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATA

AGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTG

GAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGA

AAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCG

GCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCC

TGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCG

ATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCA

ACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATG

TTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTT

TGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGT

CAGTGAGCGAGGAAGCGGATGAGCGCCCAATACGCAAACCGCCTCTCCCC

GCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTG

GAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCATT

AGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGA

ATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTA

CGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCG

AGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCC

TGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCT

GGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGC

AGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCT

GTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTG

ATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCG

CCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGAC

[Image Omitted]

GCCGCACGAATCCATGTGGGAGTTTATTCTTGACACAGATATTTATGATA

TAATAACTGAGTAAGCTTAACATAAGGAGGAAAAACATATGTTACGCAGC

AGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAGGTGG

CTCAAGTATGGGCATCATTCGCACATGTAGGCTCGGCCCTGACCAAGTCA

AATCCATGCGGGCTGCTCTTGATCTTTTCGGTCGTGAGTTCGGAGACGTA

GCCACCTACTCCCAACATCAGCCGGACTCCGATTACCTCGGGAACTTGCT

CCGTAGTAAGACATTCATCGCGCTTGCTGCCTTCGACCAAGAAGCGGTTG

TTGGCGCTCTCGCGGCTTACGTTCTGCCCAAGTTTGAGCAGCCGCGTAGT

GAGATCTATATCTATGATCTCGCAGTCTCCGGCGAGCACCGGAGGCAGGG

CATTGCCACCGCGCTCATCAATCTCCTCAAGCATGAGGCCAACGCGCTTG

GTGCTTATGTGATCTACGTGCAAGCAGATTACGGTGACGATCCCGCAGTG

GCTCTCTATACAAAGTTGGGCATACGGGAAGAAGTGATGCACTTTGATAT

CGACCCAAGTACCGCCACCTAAGAGCTGGAGCGGCGTAACCGTCGCACAG

GAAGGACAGAGAAAGCGCGGATCTGGGAAGTGACGGACAGAACGGTCAGG

ACCTGGATTGGGGAGGCGGTTGCCGCCGCTGCTGCTGACGGTGTGACGTT

CTCTGTTCCGGTCACACCACATACGTTCCGCCATTCCTATGCGATGCACA

TGCTGTATGCCGGTATACCGCTGAAAGTTCTGCAAAGCCTGATGGGACAT

AAGTCCATCAGTTCAACGGAAGTCTACACGAAGGTTTTTGCGCTGGATGT

GGCTGCCCGGCTCCGGGTGCAGTTTGCGATGCCGGAGTCTGATGCGGTTG

CGATGCTGAAACAATTATCCTGAGAATAAATGCCTTGGCCTTTATATGGA

AATGTGGAACTGAGTGGATATGCTGTTTTTGTCTGTTAAACAGAGAAGCT

GGCTGTTATCCACTGAGAAGCGAACGAAACAGTCGGGAAAATCTCCCATT

ATCGTAGAGATCCGCATTATTAATCTCAGGAGCCTGTGTAGCGTTTATAG

GAAGTAGTGTTCTGTCATGATGCCTGCAAGCGGTAACGAAAACGATTTGA

ATATGCCTTCAGGAACAATAGAAATCTTCGTGCGGTGTTACGTTGAAGTG

GAGCGGATTATGTCAGCAATGGACAGAACAACCTAATGAACACAGAACCA

TGATGTGGTCTGTCCTTTTACAGCCAGTAGTGCTCGCCGCAGTCGAGCGA

CAGGGCGAAGCCCTCGAGTGAGCGAGGAAGCACCAGGGAACAGCACTTAT

ATATTCTGCTTACACACGATGCCTGAAAAAACTTCCCTTGGGGTTATCCA

CTTATCCACGGGGATATTTTTATAATTATTTTTTTTATAGTTTTTAGATC

TTCTTTTTTAGAGCGCCTTGTAGGCCTTTATCCATGCTGGTTCTAGAGAA

GGTGTTGTGACAAATTGCCCTTTCAGTGTGACAAATCACCCTCAAATGAC

AGTCCTGTCTGTGACAAATTGCCCTTAACCCTGTGACAAATTGCCCTCAG

AAGAAGCTGTTTTTTCACAAAGTTATCCCTGCTTATTGACTCTTTTTTAT

TTAGTGTGACAATCTAAAAACTTGTCACACTTCACATGGATCTGTCATGG

CGGAAACAGCGGTTATCAATCACAAGAAACGTAAAAATAGCCCGCGAATC

GTCCAGTCAAACGACCTCACTGAGGCGGCATATAGTCTCTCCCGGGATCA

AAAACGTATGCTGTATCTGTTCGTTGACCAGATCAGAAAATCTGATGGCA

CCCTACAGGAACATGACGGTATCTGCGAGATCCATGTTGCTAAATATGCT

GAAATATTCGGATTGACCTCTGCGGAAGCCAGTAAGGATATACGGCAGGC

ATTGAAGAGTTTCGCGGGGAAGGAAGTGGTTTTTTATCGCCCTGAAGAGG

ATGCCGGCGATGAAAAAGGCTATGAATCTTTTCCTTGGTTTATCAAACGT

GCGCACAGTCCATCCAGAGGGCTTTACAGTGTACATATCAACCCATATCT

CATTCCCTTCTTTATCGGGTTACAGAACCGGTTTACGCAGTTTCGGCTTA

GTGAAACAAAAGAAATCACCAATCCGTATGCCATGCGTTTATACGAATCC

CTGTGTCAGTATCGTAAGCCGGATGGCTCAGGCATCGTGTCTCTGAAAAT

CGACTGGATCATAGAGCGTTACCAGCTGCCTCAAAGTTACCAGCGTATGC

CTGACTTCCGCCGCCGCTTCCTGCAGGTCTGTGTTAATGAGATCAACAGC

AGAACTCCAATGCGCCTCTCATACATTGAGAAAAAGAAAGGCCGCCAGAC

GACTCATATCGTATTTTCCTTCCGCGATATCACTTCCATGACGACAGGAT

AGTCTGAGGGTTATCTGTCACAGATTTGAGGGTGGTTCGTCACATTTGTT

CTGACCTACTGAGGGTAATTTGTCACAGTTTTGCTGTTTCCTTCAGCCTG

CATGGATTTTCTCATACTTTTTGAACTGTAATTTTTAAGGAAGCCAAATT

TGAGGGCAGTTTGTCACAGTTGATTTCCTTCTCTTTCCCTTCGTCATGTG

ACCTGATATCGGGGGTTAGTTCGTCATCATTGATGAGGGTTGATTATCAC

AGTTTATTACTCTGAATTGGCTATCCGCGTGTGTACCTCTACCTGGAGTT

TTTCCCACGGTGGATATTTCTTCTTGCGCTGAGCGTAAGAGCTATCTGAC

AGAACAGTTCTTCTTTGCTTCCTCGCCAGTTCGCTCGCTATGCTCGGTTA

CACGGCTGCGGCGAGCGCTAGTGATAATAAGTGACTGAGGTATGTGGTGT

TCTTATCTCCTTTTGTAGTGTTGCTCTTATTTTAAACAACTTTGCGGTTT

TTTGATGACTTTGCGATTTTGTTGTTGCTTTGCAGTAAATTGCAAGATTT

AATAAAAAAACGCAAAGCAATGATTAAAGGATGTTCAGAATGAAACTCAT

GGAAACACTTAACCAGTGCATAAACGCTGGTCATGAAATGACGAAGGCTA

TCGCCATTGCACAGTTTAATGATGACAGCCCGGAAGCGAGGAAAATAACC

CGGCGCTGGAGAATAGGTGAAGCAGCGGATTTAGTTGGGGTTTCTTCTCA

GGCTATCAGAGATGCCGAGAAAGCAGGGCGACTACCGCACCCGGATATGG

AAATTCGAGGACGGGTTGAGCAACGTGTTGGTTATACAATTGAACAAATT

AATCATATGCGTGATGTGTTTGGTACGCGATTGCGACGTGCTGAGGACGT

ATTTCCACCGGTGATCGGGGTTGCTGCCCATAAAGGTGGCGTTTACAAAA

CCTCAGTTTCTGTTCATCTTGCTCAGGATCTGGCTCTGAAGGGGCTACGT

GTTTTGCTCGTGGAAGGTAACGACCCCCAGGGAACAGCCTCAATGTATCA

CGGATGGGTACCAGATCTTCATATTCATGCAGAAGATACTCTCCTGCCTT

TCTATCTTGGGGAAAAGGACGATGTCACTTATGCAATAAAGCCCACTTGC

TGGCCGGGGCTTGACATTATTCCTTCCTGTCTGGCTCTGCACCGTATTGA

AACTGAGTTAATGGGCAAATTTGATGAAGGTAAACTGCCCACCGATCCAC

ACCTGATGCTCCGACTGGCCATTGAAACTGTTGCTCATGACTATGATGTC

ATAGTTATTGACAGCGCGCCTAACCTGGGTATCGGCACGATTAATGTCGT

ATGTGCTGCTGATGTGCTGATTGTTCCCACGCCTGCTGAGTIGTTTGACT

ACACCTCCGCACTGCAGTTTTTCGATATGCTTCGTGATCTGCTCAAGAAC

GTTGATCTTAAAGGGTTCGAGCCTGATGTACGTATTTTGCTTACCAAATA

CAGCAATAGTAATGGCTCTCAGTCCCCGTGGATGGAGGAGCAAATTCGGG

ATGCCTGGGGAAGCATGGTTCTAAAAAATGTTGTACGTGAAACGGATGAA

GTTGGTAAAGGTCAGATCCGGATGAGAACTGTTTTTGAACAGGCCATTGA

TCAACGCAGTTCAACTGGTGCCTGGAGAAATGCTCTTTCTATTTGGGAAC

CTGTCTGCAATGAAATTTTCGATCGTCTGATTAAACCACGCTGGGAGATT

AGATAATGAAGCGTGCGCCTGTTATTCCAAAACATACGCTCAATACTCAA

CCGGTTGAAGATACTTCGTTATCGACACCAGCTGCCCCGATGGTGGATTC

GTTAATTGCGCGCGTAGGAGTAATGGCTCGCGGTAATGCCATTACTTTGC

CTGTATGTGGTCGGGATGTGAAGTTTACTCTTGAAGTGCTCCGGGGTGAT

AGTGTTGAGAAAACCTCTCGGGTATGGTCAGGTAATGAACGTGACCAGGA

GCTGCTTACTGAGGACGCACTGGATGATCTCATCCCTTCTTTTCTACTGA

CTGGTCAACAGACACCGGCGTTCGGTCGAAGAGTATCTGGTGTCATAGAA

ATTGCCGATGGGAGTCGCCGTCGTAAAGCTGCTGCACTTACCGAAAGTGA

TTATCGTGTTCTGGTTGGCGAGCTGGATGATGAGCAGATGGCTGCATTAT

CCAGATTGGGTAACGATTATCGCCCAACAAGTGCTTATGAACGTGGTCAG

CGTTATGCAAGCCGATTGCAGAATGAATTTGCTGGAAATATTTCTGCGCT

GGCTGATGCGGAAAATATTTCACGTAAGATTATTACCCGCTGTATCAACA

CCGCCAAATTGCCTAAATCAGTTGTTGCTCTTTTTTCTCACCCCGGTGAA

CTATCTGCCCGGTCAGGTGATGCACTTCAAAAAGCCTTTACAGATAAAGA

GGAATTACTTAAGCAGCAGGCATCTAACCTTCATGAGCAGAAAAAAGCTG

GGGTGATATTTGAAGCTGAAGAAGTTATCACTCTTTTAACTTCTGTGCTT

AAAACGTCATCTGCATCAAGAACTAGTTTAAGCTCACGACATCAGTTTGC

TCCTGGAGCGACAGTATTGTATAAGGGCGATAAAATGGTGCTTAACCTGG

ACAGGTCACGTGTTCCAACTGAGTGTATAGAGAAAATTGAGGCCATTCTT

AAGGAACTTGAAAAGCCAGCACCCTGATGCGACCACGTTTTAGTCTACGT

TTATCTGTCTTTACTTAATGTCCTTTGTTACAGGCCAGAAAGCATAACTG

GCCTGAATATTCTCTCTGGGCCCACTGTTCCACTTGTATCGTCGGTCTGA

TAATCAGACTGGGACCACGGTCCCACTCGTATCGTCGGTCTGATTATTAG

TCTGGGACCACGGTCCCACTCGTATCGTCGGTCTGATTATTAGTCTGGGA

CCACGGTCCCACTCGTATCGTCGGTCTGATAATCAGACTGGGACCACGGT

CCCACTCGTATCGTCGGTCTGATTATTAGTCTGGGACCATGGTCCCACTC

GTATCGTCGGTCTGATTATTAGTCTGGGACCACGGTCCCACTCGTATCGT

CGGTCTGATTATTAGTCTGGAACCACGGTCCCACTCGTATCGTCGGTCTG

ATTATTAGTCTGGGACCACGGTCCCACTCGTATCGTCGGTCTGATTATTA

GTCTGGGACCACGATCCCACTCGTGTTGTCGGTCTGATTATCGGTCTGGG

ACCACGGTCCCACTTGTATTGTCGATCAGACTATCAGCGTGAGACTACGA

TTCCATCAATGCCTGTCAAGGGCAAGTATTGACATGTCGTCGTAACCTGT

AGAACGGAGTAACCTCGGTGTGCGGTTGTATGCCTGCTGTGGATTGCTGC

TGTGTCCTGCTTATCCACAACATTTTGCGCACGGTTATGTGGACAAAATA

CCTGGTTACCCAGGCCGTGCCGGCACGTTAACCGGGCTGCATCCGATGCA

AGTGTGTCGCTGTCGACGCAAGCGGCCGCA

>pMXBG_I7G

(SEQ ID NO: 44)

[Image Omitted]

CATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATC

TAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGA

GTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTT

CTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAA

CCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCT

TTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCC

TTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCG

CCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGG

CGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATA

AGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTG

GAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGA

AAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCG

GCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCC

TGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCG

ATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCA

ACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATG

TTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTT

TGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGT

CAGTGAGCGAGGAAGCGGATGAGCGCCCAATACGCAAACCGCCTCTCCCC

GCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTG

GAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCATT

AGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGA

ATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTA

CGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCG

AGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCC

TGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCT

GGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGC

AGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCT

GTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTG

ATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCG

CCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGAC

[Image Omitted]

GCCGCACGAATCCATGTGGGAGTTTATTCTTGACACAGATATTTATGATA

TAATAACTGAGTAAGCTTAACATAAGGAGGAAAAACATATGTTACGCAGC

AGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAGGTGG

CTCAAGTATGGGCATCATTCGCACATGTAGGCTCGGCCCTGACCAAGTCA

AATCCATGCGGGCTGCTCTTGATCTTTTCGGTCGTGAGTTCGGAGACGTA

GCCACCTACTCCCAACATCAGCCGGACTCCGATTACCTCGGGAACTTGCT

CCGTAGTAAGACATTCATCGCGCTTGCTGCCTTCGACCAAGAAGCGGTTG

TTGGCGCTCTCGCGGCTTACGTTCTGCCCAAGTTTGAGCAGCCGCGTAGT

GAGATCTATATCTATGATCTCGCAGTCTCCGGCGAGCACCGGAGGCAGGG

CATTGCCACCGCGCTCATCAATCTCCTCAAGCATGAGGCCAACGCGCTTG

GTGCTTATGTGATCTACGTGCAAGCAGATTACGGTGACGATCCCGCAGTG

GCTCTCTATACAAAGTTGGGCATACGGGAAGAAGTGATGCACTTTGATAT

CGACCCAAGTACCGCCACCTAAGAGCTGGAGCGGCGTAACCGTCGCACAG

GAAGGACAGAGAAAGCGCGGATCTGGGAAGTGACGGACAGAACGGTCAGG

ACCTGGATTGGGGAGGCGGTTGCCGCCGCTGCTGCTGACGGTGTGACGTT

CTCTGTTCCGGTCACACCACATACGTTCCGCCATTCCTATGCGATGCACA

TGCTGTATGCCGGTATACCGCTGAAAGTTCTGCAAAGCCTGATGGGACAT

AAGTCCATCAGTTCAACGGAAGTCTACACGAAGGTTTTTGCGCTGGATGT

GGCTGCCCGGCTCCGGGTGCAGTTTGCGATGCCGGAGTCTGATGCGGTTG

CGATGCTGAAACAATTATCCTGAGAATAAATGCCTTGGCCTTTATATGGA

AATGTGGAACTGAGTGGATATGCTGTTTTTGTCTGTTAAACAGAGAAGCT

GGCTGTTATCCACTGAGAAGCGAACGAAACAGTCGGGAAAATCTCCCATT

ATCGTAGAGATCCGCATTATTAATCTCAGGAGCCTGTGTAGCGTTTATAG

GAAGTAGTGTTCTGTCATGATGCCTGCAAGCGGTAACGAAAACGATTTGA

ATATGCCTTCAGGAACAATAGAAATCTTCGTGCGGTGTTACGTTGAAGTG

GAGCGGATTATGTCAGCAATGGACAGAACAACCTAATGAACACAGAACCA

TGATGTGGTCTGTCCTTTTACAGCCAGTAGTGCTCGCCGCAGTCGAGCGA

CAGGGCGAAGCCCTCGAGTGAGCGAGGAAGCACCAGGGAACAGCACTTAT

ATATTCTGCTTACACACGATGCCTGAAAAAACTTCCCTTGGGGTTATCCA

CTTATCCACGGGGATATTTTTATAATTATTTTTTTTATAGTTTTTAGATC

TTCTTTTTTAGAGCGCCTTGTAGGCCTTTATCCATGCTGGTTCTAGAGAA

GGTGTTGTGACAAATTGCCCTTTCAGTGTGACAAATCACCCTCAAATGAC

AGTCCTGTCTGTGACAAATTGCCCTTAACCCTGTGACAAATTGCCCTCAG

AAGAAGCTGTTTTTTCACAAAGTTATCCCTGCTTATTGACTCTTTTTTAT

TTAGTGTGACAATCTAAAAACTTGTCACACTTCACATGGATCTGTCATGG

CGGAAACAGCGGTTATCAATCACAAGAAACGTAAAAATAGCCCGCGAATC

GTCCAGTCAAACGACCTCACTGAGGCGGCATATAGTCTCTCCCGGGATCA

AAAACGTATGCTGTATCTGTTCGTTGACCAGATCAGAAAATCTGATGGCA

CCCTACAGGAACATGACGGTATCTGCGAGATCCATGTTGCTAAATATGCT

GAAATATTCGGATTGACCTCTGCGGAAGCCAGTAAGGATATACGGCAGGC

ATTGAAGAGTTTCGCGGGGAAGGAAGTGGTTTTTTATCGCCCTGAAGAGG

ATGCCGGCGATGAAAAAGGCTATGAATCTTTTCCTTGGTTTATCAAACGT

GCGCACAGTCCATCCAGAGGGCTTTACAGTGTACATATCAACCCATATCT

CATTCCCTTCTTTATCGGGTTACAGAACCGGTTTACGCAGTTTCGGCTTA

GTGAAACAAAAGAAATCACCAATCCGTATGCCATGCGTTTATACGAATCC

CTGTGTCAGTATCGTAAGCCGGATGGCTCAGGCATCGTGTCTCTGAAAAT

CGACTGGATCATAGAGCGTTACCAGCTGCCTCAAAGTTACCAGCGTATGC

CTGACTTCCGCCGCCGCTTCCTGCAGGTCTGTGTTAATGAGATCAACAGC

AGAACTCCAATGCGCCTCTCATACATTGAGAAAAAGAAAGGCCGCCAGAC

GACTCATATCGTATTTTCCTTCCGCGATATCACTTCCATGACGACAGGAT

AGTCTGAGGGTTATCTGTCACAGATTTGAGGGTGGTTCGTCACATTTGTT

CTGACCTACTGAGGGTAATTTGTCACAGTTTTGCTGTTTCCTTCAGCCTG

CATGGATTTTCTCATACTTTTTGAACTGTAATTTTTAAGGAAGCCAAATT

TGAGGGCAGTTTGTCACAGTTGATTTCCTTCTCTTTCCCTTCGTCATGTG

ACCTGATATCGGGGGTTAGTTCGTCATCATTGATGAGGGTTGATTATCAC

AGTTTATTACTCTGAATTGGCTATCCGCGTGTGTACCTCTACCTGGAGTT

TTTCCCACGGTGGATATTTCTTCTTGCGCTGAGCGTAAGAGCTATCTGAC

AGAACAGTTCTTCTTTGCTTCCTCGCCAGTTCGCTCGCTATGCTCGGTTA

CACGGCTGCGGCGAGCGCTAGTGATAATAAGTGACTGAGGTATGTGGTGT

TCTTATCTCCTTTTGTAGTGTTGCTCTTATTTTAAACAACTTTGCGGTTT

TTTGATGACTTTGCGATTTTGTTGTTGCTTTGCAGTAAATTGCAAGATTT

AATAAAAAAACGCAAAGCAATGATTAAAGGATGTTCAGAATGAAACTCAT

GGAAACACTTAACCAGTGCATAAACGCTGGTCATGAAATGACGAAGGCTA

TCGCCATTGCACAGTTTAATGATGACAGCCCGGAAGCGAGGAAAATAACC

CGGCGCTGGAGAATAGGTGAAGCAGCGGATTTAGTTGGGGTTTCTTCTCA

GGCTATCAGAGATGCCGAGAAAGCAGGGCGACTACCGCACCCGGATATGG

AAATTCGAGGACGGGTTGAGCAACGTGTTGGTTATACAATTGAACAAATT

AATCATATGCGTGATGTGTTTGGTACGCGATTGCGACGTGCTGAGGACGT

ATTTCCACCGGTGATCGGGGTTGCTGCCCATAAAGGTGGCGTTTACAAAA

CCTCAGTTTCTGTTCATCTTGCTCAGGATCTGGCTCTGAAGGGGCTACGT

GTTTTGCTCGTGGAAGGTAACGACCCCCAGGGAACAGCCTCAATGTATCA

CGGATGGGTACCAGATCTTCATATTCATGCAGAAGATACTCTCCTGCCTT

TCTATCTTGGGGAAAAGGACGATGTCACTTATGCAATAAAGCCCACTTGC

TGGCCGGGGCTTGACATTATTCCTTCCTGTCTGGCTCTGCACCGTATTGA

AACTGAGTTAATGGGCAAATTTGATGAAGGTAAACTGCCCACCGATCCAC

ACCTGATGCTCCGACTGGCCATTGAAACTGTTGCTCATGACTATGATGTC

ATAGTTATTGACAGCGCGCCTAACCTGGGTATCGGCACGATTAATGTCGT

ATGTGCTGCTGATGTGCTGATTGTTCCCACGCCTGCTGAGTIGTTTGACT

ACACCTCCGCACTGCAGTTTTTCGATATGCTTCGTGATCTGCTCAAGAAC

GTTGATCTTAAAGGGTTCGAGCCTGATGTACGTATTTTGCTTACCAAATA

CAGCAATAGTAATGGCTCTCAGTCCCCGTGGATGGAGGAGCAAATTCGGG

ATGCCTGGGGAAGCATGGTTCTAAAAAATGTTGTACGTGAAACGGATGAA

GTTGGTAAAGGTCAGATCCGGATGAGAACTGTTTTTGAACAGGCCATTGA

TCAACGCAGTTCAACTGGTGCCTGGAGAAATGCTCTTTCTATTTGGGAAC

CTGTCTGCAATGAAATTTTCGATCGTCTGATTAAACCACGCTGGGAGATT

AGATAATGAAGCGTGCGCCTGTTATTCCAAAACATACGCTCAATACTCAA

CCGGTTGAAGATACTTCGTTATCGACACCAGCTGCCCCGATGGTGGATTC

GTTAATTGCGCGCGTAGGAGTAATGGCTCGCGGTAATGCCATTACTTTGC

CTGTATGTGGTCGGGATGTGAAGTTTACTCTTGAAGTGCTCCGGGGTGAT

AGTGTTGAGAAAACCTCTCGGGTATGGTCAGGTAATGAACGTGACCAGGA

GCTGCTTACTGAGGACGCACTGGATGATCTCATCCCTTCTTTTCTACTGA

CTGGTCAACAGACACCGGCGTTCGGTCGAAGAGTATCTGGTGTCATAGAA

ATTGCCGATGGGAGTCGCCGTCGTAAAGCTGCTGCACTTACCGAAAGTGA

TTATCGTGTTCTGGTTGGCGAGCTGGATGATGAGCAGATGGCTGCATTAT

CCAGATTGGGTAACGATTATCGCCCAACAAGTGCTTATGAACGTGGTCAG

CGTTATGCAAGCCGATTGCAGAATGAATTTGCTGGAAATATTTCTGCGCT

GGCTGATGCGGAAAATATTTCACGTAAGATTATTACCCGCTGTATCAACA

CCGCCAAATTGCCTAAATCAGTTGTTGCTCTTTTTTCTCACCCCGGTGAA

CTATCTGCCCGGTCAGGTGATGCACTTCAAAAAGCCTTTACAGATAAAGA

GGAATTACTTAAGCAGCAGGCATCTAACCTTCATGAGCAGAAAAAAGCTG

GGGTGATATTTGAAGCTGAAGAAGTTATCACTCTTTTAACTTCTGTGCTT

AAAACGTCATCTGCATCAAGAACTAGTTTAAGCTCACGACATCAGTTTGC

TCCTGGAGCGACAGTATTGTATAAGGGCGATAAAATGGTGCTTAACCTGG

ACAGGTCACGTGTTCCAACTGAGTGTATAGAGAAAATTGAGGCCATTCTT

AAGGAACTTGAAAAGCCAGCACCCTGATGCGACCACGTTTTAGTCTACGT

TTATCTGTCTTTACTTAATGTCCTTTGTTACAGGCCAGAAAGCATAACTG

GCCTGAATATTCTCTCTGGGCCCACTGTTCCACTTGTATCGTCGGTCTGA

TAATCAGACTGGGACCACGGTCCCACTCGTATCGTCGGTCTGATTATTAG

TCTGGGACCACGGTCCCACTCGTATCGTCGGTCTGATTATTAGTCTGGGA

CCACGGTCCCACTCGTATCGTCGGTCTGATAATCAGACTGGGACCACGGT

CCCACTCGTATCGTCGGTCTGATTATTAGTCTGGGACCATGGTCCCACTC

GTATCGTCGGTCTGATTATTAGTCTGGGACCACGGTCCCACTCGTATCGT

CGGTCTGATTATTAGTCTGGAACCACGGTCCCACTCGTATCGTCGGTCTG

ATTATTAGTCTGGGACCACGGTCCCACTCGTATCGTCGGTCTGATTATTA

GTCTGGGACCACGATCCCACTCGTGTTGTCGGTCTGATTATCGGTCTGGG

ACCACGGTCCCACTTGTATTGTCGATCAGACTATCAGCGTGAGACTACGA

TTCCATCAATGCCTGTCAAGGGCAAGTATTGACATGTCGTCGTAACCTGT

AGAACGGAGTAACCTCGGTGTGCGGTTGTATGCCTGCTGTGGATTGCTGC

TGTGTCCTGCTTATCCACAACATTTTGCGCACGGTTATGTGGACAAAATA

CCTGGTTACCCAGGCCGTGCCGGCACGTTAACCGGGCTGCATCCGATGCA

AGTGTGTCGCTGTCGACGCAAGCGGCCGCA

>pMXBG_G7F

(SEQ ID NO: 45)

[Image Omitted]

CATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATC

TAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGA

GTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTT

CTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAA

CCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCT

TTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCC

TTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCG

CCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGG

CGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATA

AGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTG

GAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGA

AAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCG

GCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCC

TGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCG

ATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCA

ACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATG

TTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTT

TGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGT

CAGTGAGCGAGGAAGCGGATGAGCGCCCAATACGCAAACCGCCTCTCCCC

GCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTG

GAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCATT

AGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGA

ATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTA

CGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCG

AGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCC

TGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCT

GGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGC

AGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCT

GTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTG

ATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCG

CCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGAC

[Image Omitted]

GCCGCACGAATCCATGTGGGAGTTTATTCTTGACACAGATATTTATGATA

TAATAACTGAGTAAGCTTAACATAAGGAGGAAAAACATATGTTACGCAGC

AGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAGGTGG

CTCAAGTATGGGCATCATTCGCACATGTAGGCTCGGCCCTGACCAAGTCA

AATCCATGCGGGCTGCTCTTGATCTTTTCGGTCGTGAGTTCGGAGACGTA

GCCACCTACTCCCAACATCAGCCGGACTCCGATTACCTCGGGAACTTGCT

CCGTAGTAAGACATTCATCGCGCTTGCTGCCTTCGACCAAGAAGCGGTTG

TTGGCGCTCTCGCGGCTTACGTTCTGCCCAAGTTTGAGCAGCCGCGTAGT

GAGATCTATATCTATGATCTCGCAGTCTCCGGCGAGCACCGGAGGCAGGG

CATTGCCACCGCGCTCATCAATCTCCTCAAGCATGAGGCCAACGCGCTTG

GTGCTTATGTGATCTACGTGCAAGCAGATTACGGTGACGATCCCGCAGTG

GCTCTCTATACAAAGTTGGGCATACGGGAAGAAGTGATGCACTTTGATAT

CGACCCAAGTACCGCCACCTAAGAGCTGGAGCGGCGTAACCGTCGCACAG

GAAGGACAGAGAAAGCGCGGATCTGGGAAGTGACGGACAGAACGGTCAGG

ACCTGGATTGGGGAGGCGGTTGCCGCCGCTGCTGCTGACGGTGTGACGTT

CTCTGTTCCGGTCACACCACATACGTTCCGCCATTCCTATGCGATGCACA

TGCTGTATGCCGGTATACCGCTGAAAGTTCTGCAAAGCCTGATGGGACAT

AAGTCCATCAGTTCAACGGAAGTCTACACGAAGGTTTTTGCGCTGGATGT

GGCTGCCCGGCTCCGGGTGCAGTTTGCGATGCCGGAGTCTGATGCGGTTG

CGATGCTGAAACAATTATCCTGAGAATAAATGCCTTGGCCTTTATATGGA

AATGTGGAACTGAGTGGATATGCTGTTTTTGTCTGTTAAACAGAGAAGCT

GGCTGTTATCCACTGAGAAGCGAACGAAACAGTCGGGAAAATCTCCCATT

ATCGTAGAGATCCGCATTATTAATCTCAGGAGCCTGTGTAGCGTTTATAG

GAAGTAGTGTTCTGTCATGATGCCTGCAAGCGGTAACGAAAACGATTTGA

ATATGCCTTCAGGAACAATAGAAATCTTCGTGCGGTGTTACGTTGAAGTG

GAGCGGATTATGTCAGCAATGGACAGAACAACCTAATGAACACAGAACCA

TGATGTGGTCTGTCCTTTTACAGCCAGTAGTGCTCGCCGCAGTCGAGCGA

CAGGGCGAAGCCCTCGAGTGAGCGAGGAAGCACCAGGGAACAGCACTTAT

ATATTCTGCTTACACACGATGCCTGAAAAAACTTCCCTTGGGGTTATCCA

CTTATCCACGGGGATATTTTTATAATTATTTTTTTTATAGTTTTTAGATC

TTCTTTTTTAGAGCGCCTTGTAGGCCTTTATCCATGCTGGTTCTAGAGAA

GGTGTTGTGACAAATTGCCCTTTCAGTGTGACAAATCACCCTCAAATGAC

AGTCCTGTCTGTGACAAATTGCCCTTAACCCTGTGACAAATTGCCCTCAG

AAGAAGCTGTTTTTTCACAAAGTTATCCCTGCTTATTGACTCTTTTTTAT

TTAGTGTGACAATCTAAAAACTTGTCACACTTCACATGGATCTGTCATGG

CGGAAACAGCGGTTATCAATCACAAGAAACGTAAAAATAGCCCGCGAATC

GTCCAGTCAAACGACCTCACTGAGGCGGCATATAGTCTCTCCCGGGATCA

AAAACGTATGCTGTATCTGTTCGTTGACCAGATCAGAAAATCTGATGGCA

CCCTACAGGAACATGACGGTATCTGCGAGATCCATGTTGCTAAATATGCT

GAAATATTCGGATTGACCTCTGCGGAAGCCAGTAAGGATATACGGCAGGC

ATTGAAGAGTTTCGCGGGGAAGGAAGTGGTTTTTTATCGCCCTGAAGAGG

ATGCCGGCGATGAAAAAGGCTATGAATCTTTTCCTTGGTTTATCAAACGT

GCGCACAGTCCATCCAGAGGGCTTTACAGTGTACATATCAACCCATATCT

CATTCCCTTCTTTATCGGGTTACAGAACCGGTTTACGCAGTTTCGGCTTA

GTGAAACAAAAGAAATCACCAATCCGTATGCCATGCGTTTATACGAATCC

CTGTGTCAGTATCGTAAGCCGGATGGCTCAGGCATCGTGTCTCTGAAAAT

CGACTGGATCATAGAGCGTTACCAGCTGCCTCAAAGTTACCAGCGTATGC

CTGACTTCCGCCGCCGCTTCCTGCAGGTCTGTGTTAATGAGATCAACAGC

AGAACTCCAATGCGCCTCTCATACATTGAGAAAAAGAAAGGCCGCCAGAC

GACTCATATCGTATTTTCCTTCCGCGATATCACTTCCATGACGACAGGAT

AGTCTGAGGGTTATCTGTCACAGATTTGAGGGTGGTTCGTCACATTTGTT

CTGACCTACTGAGGGTAATTTGTCACAGTTTTGCTGTTTCCTTCAGCCTG

CATGGATTTTCTCATACTTTTTGAACTGTAATTTTTAAGGAAGCCAAATT

TGAGGGCAGTTTGTCACAGTTGATTTCCTTCTCTTTCCCTTCGTCATGTG

ACCTGATATCGGGGGTTAGTTCGTCATCATTGATGAGGGTTGATTATCAC

AGTTTATTACTCTGAATTGGCTATCCGCGTGTGTACCTCTACCTGGAGTT

TTTCCCACGGTGGATATTTCTTCTTGCGCTGAGCGTAAGAGCTATCTGAC

AGAACAGTTCTTCTTTGCTTCCTCGCCAGTTCGCTCGCTATGCTCGGTTA

CACGGCTGCGGCGAGCGCTAGTGATAATAAGTGACTGAGGTATGTGGTGT

TCTTATCTCCTTTTGTAGTGTTGCTCTTATTTTAAACAACTTTGCGGTTT

TTTGATGACTTTGCGATTTTGTTGTTGCTTTGCAGTAAATTGCAAGATTT

AATAAAAAAACGCAAAGCAATGATTAAAGGATGTTCAGAATGAAACTCAT

GGAAACACTTAACCAGTGCATAAACGCTGGTCATGAAATGACGAAGGCTA

TCGCCATTGCACAGTTTAATGATGACAGCCCGGAAGCGAGGAAAATAACC

CGGCGCTGGAGAATAGGTGAAGCAGCGGATTTAGTTGGGGTTTCTTCTCA

GGCTATCAGAGATGCCGAGAAAGCAGGGCGACTACCGCACCCGGATATGG

AAATTCGAGGACGGGTTGAGCAACGTGTTGGTTATACAATTGAACAAATT

AATCATATGCGTGATGTGTTTGGTACGCGATTGCGACGTGCTGAGGACGT

ATTTCCACCGGTGATCGGGGTTGCTGCCCATAAAGGTGGCGTTTACAAAA

CCTCAGTTTCTGTTCATCTTGCTCAGGATCTGGCTCTGAAGGGGCTACGT

GTTTTGCTCGTGGAAGGTAACGACCCCCAGGGAACAGCCTCAATGTATCA

CGGATGGGTACCAGATCTTCATATTCATGCAGAAGATACTCTCCTGCCTT

TCTATCTTGGGGAAAAGGACGATGTCACTTATGCAATAAAGCCCACTTGC

TGGCCGGGGCTTGACATTATTCCTTCCTGTCTGGCTCTGCACCGTATTGA

AACTGAGTTAATGGGCAAATTTGATGAAGGTAAACTGCCCACCGATCCAC

ACCTGATGCTCCGACTGGCCATTGAAACTGTTGCTCATGACTATGATGTC

ATAGTTATTGACAGCGCGCCTAACCTGGGTATCGGCACGATTAATGTCGT

ATGTGCTGCTGATGTGCTGATTGTTCCCACGCCTGCTGAGTIGTTTGACT

ACACCTCCGCACTGCAGTTTTTCGATATGCTTCGTGATCTGCTCAAGAAC

GTTGATCTTAAAGGGTTCGAGCCTGATGTACGTATTTTGCTTACCAAATA

CAGCAATAGTAATGGCTCTCAGTCCCCGTGGATGGAGGAGCAAATTCGGG

ATGCCTGGGGAAGCATGGTTCTAAAAAATGTTGTACGTGAAACGGATGAA

GTTGGTAAAGGTCAGATCCGGATGAGAACTGTTTTTGAACAGGCCATTGA

TCAACGCAGTTCAACTGGTGCCTGGAGAAATGCTCTTTCTATTTGGGAAC

CTGTCTGCAATGAAATTTTCGATCGTCTGATTAAACCACGCTGGGAGATT

AGATAATGAAGCGTGCGCCTGTTATTCCAAAACATACGCTCAATACTCAA

CCGGTTGAAGATACTTCGTTATCGACACCAGCTGCCCCGATGGTGGATTC

GTTAATTGCGCGCGTAGGAGTAATGGCTCGCGGTAATGCCATTACTTTGC

CTGTATGTGGTCGGGATGTGAAGTTTACTCTTGAAGTGCTCCGGGGTGAT

AGTGTTGAGAAAACCTCTCGGGTATGGTCAGGTAATGAACGTGACCAGGA

GCTGCTTACTGAGGACGCACTGGATGATCTCATCCCTTCTTTTCTACTGA

CTGGTCAACAGACACCGGCGTTCGGTCGAAGAGTATCTGGTGTCATAGAA

ATTGCCGATGGGAGTCGCCGTCGTAAAGCTGCTGCACTTACCGAAAGTGA

TTATCGTGTTCTGGTTGGCGAGCTGGATGATGAGCAGATGGCTGCATTAT

CCAGATTGGGTAACGATTATCGCCCAACAAGTGCTTATGAACGTGGTCAG

CGTTATGCAAGCCGATTGCAGAATGAATTTGCTGGAAATATTTCTGCGCT

GGCTGATGCGGAAAATATTTCACGTAAGATTATTACCCGCTGTATCAACA

CCGCCAAATTGCCTAAATCAGTTGTTGCTCTTTTTTCTCACCCCGGTGAA

CTATCTGCCCGGTCAGGTGATGCACTTCAAAAAGCCTTTACAGATAAAGA

GGAATTACTTAAGCAGCAGGCATCTAACCTTCATGAGCAGAAAAAAGCTG

GGGTGATATTTGAAGCTGAAGAAGTTATCACTCTTTTAACTTCTGTGCTT

AAAACGTCATCTGCATCAAGAACTAGTTTAAGCTCACGACATCAGTTTGC

TCCTGGAGCGACAGTATTGTATAAGGGCGATAAAATGGTGCTTAACCTGG

ACAGGTCACGTGTTCCAACTGAGTGTATAGAGAAAATTGAGGCCATTCTT

AAGGAACTTGAAAAGCCAGCACCCTGATGCGACCACGTTTTAGTCTACGT

TTATCTGTCTTTACTTAATGTCCTTTGTTACAGGCCAGAAAGCATAACTG

GCCTGAATATTCTCTCTGGGCCCACTGTTCCACTTGTATCGTCGGTCTGA

TAATCAGACTGGGACCACGGTCCCACTCGTATCGTCGGTCTGATTATTAG

TCTGGGACCACGGTCCCACTCGTATCGTCGGTCTGATTATTAGTCTGGGA

CCACGGTCCCACTCGTATCGTCGGTCTGATAATCAGACTGGGACCACGGT

CCCACTCGTATCGTCGGTCTGATTATTAGTCTGGGACCATGGTCCCACTC

GTATCGTCGGTCTGATTATTAGTCTGGGACCACGGTCCCACTCGTATCGT

CGGTCTGATTATTAGTCTGGAACCACGGTCCCACTCGTATCGTCGGTCTG

ATTATTAGTCTGGGACCACGGTCCCACTCGTATCGTCGGTCTGATTATTA

GTCTGGGACCACGATCCCACTCGTGTTGTCGGTCTGATTATCGGTCTGGG

ACCACGGTCCCACTTGTATTGTCGATCAGACTATCAGCGTGAGACTACGA

TTCCATCAATGCCTGTCAAGGGCAAGTATTGACATGTCGTCGTAACCTGT

AGAACGGAGTAACCTCGGTGTGCGGTTGTATGCCTGCTGTGGATTGCTGC

TGTGTCCTGCTTATCCACAACATTTTGCGCACGGTTATGTGGACAAAATA

CCTGGTTACCCAGGCCGTGCCGGCACGTTAACCGGGCTGCATCCGATGCA

AGTGTGTCGCTGTCGACGCAAGCGGCCGCA

>pMXBG_F7E

(SEQ ID NO: 46)

[Image Omitted]

CATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATC

TAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGA

GTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTT

CTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAA

CCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCT

TTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCC

TTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCG

CCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGG

CGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATA

AGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTG

GAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGA

AAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCG

GCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCC

TGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCG

ATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCA

ACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATG

TTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTT

TGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGT

CAGTGAGCGAGGAAGCGGATGAGCGCCCAATACGCAAACCGCCTCTCCCC

GCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTG

GAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCATT

AGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGA

ATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTA

CGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCG

AGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCC

TGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCT

GGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGC

AGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCT

GTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTG

ATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCG

CCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGAC

[Image Omitted]

GCCGCACGAATCCATGTGGGAGTTTATTCTTGACACAGATATTTATGATA

TAATAACTGAGTAAGCTTAACATAAGGAGGAAAAACATATGTTACGCAGC

AGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAGGTGG

CTCAAGTATGGGCATCATTCGCACATGTAGGCTCGGCCCTGACCAAGTCA

AATCCATGCGGGCTGCTCTTGATCTTTTCGGTCGTGAGTTCGGAGACGTA

GCCACCTACTCCCAACATCAGCCGGACTCCGATTACCTCGGGAACTTGCT

CCGTAGTAAGACATTCATCGCGCTTGCTGCCTTCGACCAAGAAGCGGTTG

TTGGCGCTCTCGCGGCTTACGTTCTGCCCAAGTTTGAGCAGCCGCGTAGT

GAGATCTATATCTATGATCTCGCAGTCTCCGGCGAGCACCGGAGGCAGGG

CATTGCCACCGCGCTCATCAATCTCCTCAAGCATGAGGCCAACGCGCTTG

GTGCTTATGTGATCTACGTGCAAGCAGATTACGGTGACGATCCCGCAGTG

GCTCTCTATACAAAGTTGGGCATACGGGAAGAAGTGATGCACTTTGATAT

CGACCCAAGTACCGCCACCTAAGAGCTGGAGCGGCGTAACCGTCGCACAG

GAAGGACAGAGAAAGCGCGGATCTGGGAAGTGACGGACAGAACGGTCAGG

ACCTGGATTGGGGAGGCGGTTGCCGCCGCTGCTGCTGACGGTGTGACGTT

CTCTGTTCCGGTCACACCACATACGTTCCGCCATTCCTATGCGATGCACA

TGCTGTATGCCGGTATACCGCTGAAAGTTCTGCAAAGCCTGATGGGACAT

AAGTCCATCAGTTCAACGGAAGTCTACACGAAGGTTTTTGCGCTGGATGT

GGCTGCCCGGCTCCGGGTGCAGTTTGCGATGCCGGAGTCTGATGCGGTTG

CGATGCTGAAACAATTATCCTGAGAATAAATGCCTTGGCCTTTATATGGA

AATGTGGAACTGAGTGGATATGCTGTTTTTGTCTGTTAAACAGAGAAGCT

GGCTGTTATCCACTGAGAAGCGAACGAAACAGTCGGGAAAATCTCCCATT

ATCGTAGAGATCCGCATTATTAATCTCAGGAGCCTGTGTAGCGTTTATAG

GAAGTAGTGTTCTGTCATGATGCCTGCAAGCGGTAACGAAAACGATTTGA

ATATGCCTTCAGGAACAATAGAAATCTTCGTGCGGTGTTACGTTGAAGTG

GAGCGGATTATGTCAGCAATGGACAGAACAACCTAATGAACACAGAACCA

TGATGTGGTCTGTCCTTTTACAGCCAGTAGTGCTCGCCGCAGTCGAGCGA

CAGGGCGAAGCCCTCGAGTGAGCGAGGAAGCACCAGGGAACAGCACTTAT

ATATTCTGCTTACACACGATGCCTGAAAAAACTTCCCTTGGGGTTATCCA

CTTATCCACGGGGATATTTTTATAATTATTTTTTTTATAGTTTTTAGATC

TTCTTTTTTAGAGCGCCTTGTAGGCCTTTATCCATGCTGGTTCTAGAGAA

GGTGTTGTGACAAATTGCCCTTTCAGTGTGACAAATCACCCTCAAATGAC

AGTCCTGTCTGTGACAAATTGCCCTTAACCCTGTGACAAATTGCCCTCAG

AAGAAGCTGTTTTTTCACAAAGTTATCCCTGCTTATTGACTCTTTTTTAT

TTAGTGTGACAATCTAAAAACTTGTCACACTTCACATGGATCTGTCATGG

CGGAAACAGCGGTTATCAATCACAAGAAACGTAAAAATAGCCCGCGAATC

GTCCAGTCAAACGACCTCACTGAGGCGGCATATAGTCTCTCCCGGGATCA

AAAACGTATGCTGTATCTGTTCGTTGACCAGATCAGAAAATCTGATGGCA

CCCTACAGGAACATGACGGTATCTGCGAGATCCATGTTGCTAAATATGCT

GAAATATTCGGATTGACCTCTGCGGAAGCCAGTAAGGATATACGGCAGGC

ATTGAAGAGTTTCGCGGGGAAGGAAGTGGTTTTTTATCGCCCTGAAGAGG

ATGCCGGCGATGAAAAAGGCTATGAATCTTTTCCTTGGTTTATCAAACGT

GCGCACAGTCCATCCAGAGGGCTTTACAGTGTACATATCAACCCATATCT

CATTCCCTTCTTTATCGGGTTACAGAACCGGTTTACGCAGTTTCGGCTTA

GTGAAACAAAAGAAATCACCAATCCGTATGCCATGCGTTTATACGAATCC

CTGTGTCAGTATCGTAAGCCGGATGGCTCAGGCATCGTGTCTCTGAAAAT

CGACTGGATCATAGAGCGTTACCAGCTGCCTCAAAGTTACCAGCGTATGC

CTGACTTCCGCCGCCGCTTCCTGCAGGTCTGTGTTAATGAGATCAACAGC

AGAACTCCAATGCGCCTCTCATACATTGAGAAAAAGAAAGGCCGCCAGAC

GACTCATATCGTATTTTCCTTCCGCGATATCACTTCCATGACGACAGGAT

AGTCTGAGGGTTATCTGTCACAGATTTGAGGGTGGTTCGTCACATTTGTT

CTGACCTACTGAGGGTAATTTGTCACAGTTTTGCTGTTTCCTTCAGCCTG

CATGGATTTTCTCATACTTTTTGAACTGTAATTTTTAAGGAAGCCAAATT

TGAGGGCAGTTTGTCACAGTTGATTTCCTTCTCTTTCCCTTCGTCATGTG

ACCTGATATCGGGGGTTAGTTCGTCATCATTGATGAGGGTTGATTATCAC

AGTTTATTACTCTGAATTGGCTATCCGCGTGTGTACCTCTACCTGGAGTT

TTTCCCACGGTGGATATTTCTTCTTGCGCTGAGCGTAAGAGCTATCTGAC

AGAACAGTTCTTCTTTGCTTCCTCGCCAGTTCGCTCGCTATGCTCGGTTA

CACGGCTGCGGCGAGCGCTAGTGATAATAAGTGACTGAGGTATGTGGTGT

TCTTATCTCCTTTTGTAGTGTTGCTCTTATTTTAAACAACTTTGCGGTTT

TTTGATGACTTTGCGATTTTGTTGTTGCTTTGCAGTAAATTGCAAGATTT

AATAAAAAAACGCAAAGCAATGATTAAAGGATGTTCAGAATGAAACTCAT

GGAAACACTTAACCAGTGCATAAACGCTGGTCATGAAATGACGAAGGCTA

TCGCCATTGCACAGTTTAATGATGACAGCCCGGAAGCGAGGAAAATAACC

CGGCGCTGGAGAATAGGTGAAGCAGCGGATTTAGTTGGGGTTTCTTCTCA

GGCTATCAGAGATGCCGAGAAAGCAGGGCGACTACCGCACCCGGATATGG

AAATTCGAGGACGGGTTGAGCAACGTGTTGGTTATACAATTGAACAAATT

AATCATATGCGTGATGTGTTTGGTACGCGATTGCGACGTGCTGAGGACGT

ATTTCCACCGGTGATCGGGGTTGCTGCCCATAAAGGTGGCGTTTACAAAA

CCTCAGTTTCTGTTCATCTTGCTCAGGATCTGGCTCTGAAGGGGCTACGT

GTTTTGCTCGTGGAAGGTAACGACCCCCAGGGAACAGCCTCAATGTATCA

CGGATGGGTACCAGATCTTCATATTCATGCAGAAGATACTCTCCTGCCTT

TCTATCTTGGGGAAAAGGACGATGTCACTTATGCAATAAAGCCCACTTGC

TGGCCGGGGCTTGACATTATTCCTTCCTGTCTGGCTCTGCACCGTATTGA

AACTGAGTTAATGGGCAAATTTGATGAAGGTAAACTGCCCACCGATCCAC

ACCTGATGCTCCGACTGGCCATTGAAACTGTTGCTCATGACTATGATGTC

ATAGTTATTGACAGCGCGCCTAACCTGGGTATCGGCACGATTAATGTCGT

ATGTGCTGCTGATGTGCTGATTGTTCCCACGCCTGCTGAGTTGTTTGACT

ACACCTCCGCACTGCAGTTTTTCGATATGCTTCGTGATCTGCTCAAGAAC

GTTGATCTTAAAGGGTTCGAGCCTGATGTACGTATTTTGCTTACCAAATA

CAGCAATAGTAATGGCTCTCAGTCCCCGTGGATGGAGGAGCAAATTCGGG

ATGCCTGGGGAAGCATGGTTCTAAAAAATGTTGTACGTGAAACGGATGAA

GTTGGTAAAGGTCAGATCCGGATGAGAACTGTTTTTGAACAGGCCATTGA

TCAACGCAGTTCAACTGGTGCCTGGAGAAATGCTCTTTCTATTTGGGAAC

CTGTCTGCAATGAAATTTTCGATCGTCTGATTAAACCACGCTGGGAGATT

AGATAATGAAGCGTGCGCCTGTTATTCCAAAACATACGCTCAATACTCAA

CCGGTTGAAGATACTTCGTTATCGACACCAGCTGCCCCGATGGTGGATTC

GTTAATTGCGCGCGTAGGAGTAATGGCTCGCGGTAATGCCATTACTTTGC

CTGTATGTGGTCGGGATGTGAAGTTTACTCTTGAAGTGCTCCGGGGTGAT

AGTGTTGAGAAAACCTCTCGGGTATGGTCAGGTAATGAACGTGACCAGGA

GCTGCTTACTGAGGACGCACTGGATGATCTCATCCCTTCTTTTCTACTGA

CTGGTCAACAGACACCGGCGTTCGGTCGAAGAGTATCTGGTGTCATAGAA

ATTGCCGATGGGAGTCGCCGTCGTAAAGCTGCTGCACTTACCGAAAGTGA

TTATCGTGTTCTGGTTGGCGAGCTGGATGATGAGCAGATGGCTGCATTAT

CCAGATTGGGTAACGATTATCGCCCAACAAGTGCTTATGAACGTGGTCAG

CGTTATGCAAGCCGATTGCAGAATGAATTTGCTGGAAATATTTCTGCGCT

GGCTGATGCGGAAAATATTTCACGTAAGATTATTACCCGCTGTATCAACA

CCGCCAAATTGCCTAAATCAGTTGTTGCTCTTTTTTCTCACCCCGGTGAA

CTATCTGCCCGGTCAGGTGATGCACTTCAAAAAGCCTTTACAGATAAAGA

GGAATTACTTAAGCAGCAGGCATCTAACCTTCATGAGCAGAAAAAAGCTG

GGGTGATATTTGAAGCTGAAGAAGTTATCACTCTTTTAACTTCTGTGCTT

AAAACGTCATCTGCATCAAGAACTAGTTTAAGCTCACGACATCAGTTTGC

TCCTGGAGCGACAGTATTGTATAAGGGCGATAAAATGGTGCTTAACCTGG

ACAGGTCACGTGTTCCAACTGAGTGTATAGAGAAAATTGAGGCCATTCTT

AAGGAACTTGAAAAGCCAGCACCCTGATGCGACCACGTTTTAGTCTACGT

TTATCTGTCTTTACTTAATGTCCTTTGTTACAGGCCAGAAAGCATAACTG

GCCTGAATATTCTCTCTGGGCCCACTGTTCCACTTGTATCGTCGGTCTGA

TAATCAGACTGGGACCACGGTCCCACTCGTATCGTCGGTCTGATTATTAG

TCTGGGACCACGGTCCCACTCGTATCGTCGGTCTGATTATTAGTCTGGGA

CCACGGTCCCACTCGTATCGTCGGTCTGATAATCAGACTGGGACCACGGT

CCCACTCGTATCGTCGGTCTGATTATTAGTCTGGGACCATGGTCCCACTC

GTATCGTCGGTCTGATTATTAGTCTGGGACCACGGTCCCACTCGTATCGT

CGGTCTGATTATTAGTCTGGAACCACGGTCCCACTCGTATCGTCGGTCTG

ATTATTAGTCTGGGACCACGGTCCCACTCGTATCGTCGGTCTGATTATTA

GTCTGGGACCACGATCCCACTCGTGTTGTCGGTCTGATTATCGGTCTGGG

ACCACGGTCCCACTTGTATTGTCGATCAGACTATCAGCGTGAGACTACGA

TTCCATCAATGCCTGTCAAGGGCAAGTATTGACATGTCGTCGTAACCTGT

AGAACGGAGTAACCTCGGTGTGCGGTTGTATGCCTGCTGTGGATTGCTGC

TGTGTCCTGCTTATCCACAACATTTTGCGCACGGTTATGTGGACAAAATA

CCTGGTTACCCAGGCCGTGCCGGCACGTTAACCGGGCTGCATCCGATGCA

AGTGTGTCGCTGTCGACGCAAGCGGCCGCA

>pMXBK_A7E

(SEQ ID NO: 47)

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CATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATC

TAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGA

GTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTT

CTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAA

CCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCT

TTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCC

TTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCG

CCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGG

CGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATA

AGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTG

GAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGA

AAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCG

GCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCC

TGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCG

ATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCA

ACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATG

TTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTT

TGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGT

CAGTGAGCGAGGAAGCGGATGAGCGCCCAATACGCAAACCGCCTCTCCCC

GCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTG

GAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCATT

AGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGA

ATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTA

CGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCG

AGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCC

TGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCT

GGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGC

AGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCT

GTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTG

ATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCG

CCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGAC

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GCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCG

CTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAA

GAGTATGGCTAAAATGAGAATATCACCGGAATTGAAAAAACTGATCGAAA

AATACCGCTGCGTAAAAGATACGGAAGGAATGTCTCCTGCTAAGGTATAT

AAGCTGGTGGGAGAAAATGAAAACCTATATTTAAAAATGACGGACAGCCG

GTATAAAGGGACCACCTATGATGTGGAACGGGAAAAGGACATGATGCTAT

GGCTGGAAGGAAAGCTGCCTGTTCCAAAGGTCCTGCACTTTGAACGGCAT

GATGGCTGGAGCAATCTGCTCATGAGTGAGGCCGATGGCGTCCTTTGCTC

GGAAGAGTATGAAGATGAACAAAGCCCTGAAAAGATTATCGAGCTGTATG

CGGAGTGCATCAGGCTCTTTCACTCCATCGACATATCGGATTGTCCCTAT

ACGAATAGCTTAGACAGCCGCTTAGCCGAATTGGATTACTTACTGAATAA

CGATCTGGCCGATGTGGATTGCGAAAACTGGGAAGAGGACACTCCATTTA

AAGATCCGCGCGAGCTGTATGATTTTTTAAAGACGGAAAAGCCCGAAGAG

GAACTTGTCTTTTCCCACGGCGACCTGGGAGACAGCAACATCTTTGTGAA

AGATGGCAAAGTAAGTGGCTTTATTGATCTTGGGAGAAGCGGCAGGGCGG

ACAAGTGGTATGACATTGCCTTCTGCGTCCGGTCGCTCAGGGAGGATATC

GGGGAAGAACAGTATGTCGAGCTATTTTTTGACTTACTGGGGATCAAGCC

TGATTGGGAGAAAATAAAATATTATATTTTACTGGATGAATTGTTTTAGG

AGCTGGAGCGGCGTAACCGTCGCACAGGAAGGACAGAGAAAGCGCGGATC

TGGGAAGTGACGGACAGAACGGTCAGGACCTGGATTGGGGAGGCGGTTGC

CGCCGCTGCTGCTGACGGTGTGACGTTCTCTGTTCCGGTCACACCACATA

CGTTCCGCCATTCCTATGCGATGCACATGCTGTATGCCGGTATACCGCTG

AAAGTTCTGCAAAGCCTGATGGGACATAAGTCCATCAGTTCAACGGAAGT

CTACACGAAGGTTTTTGCGCTGGATGTGGCTGCCCGGCTCCGGGTGCAGT