Patexia. Research
Patent No. US 10738349
Issue Date Aug 11, 2020
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Patent 10738349 - Polynucleotide based movement, kits and methods related thereto > Claims

  • 1. A rolling particle system comprising, a spherical particle comprising a coating of single stranded DNA;a planar substrate comprising a coating of single stranded RNA, wherein the RNA density is between about 2×104 molecules/μm2 and about 6×104 molecules/μm2, wherein the RNA is conjugated to a fluorescent molecule, wherein the substrate further comprises polyethylene glycol; andRNase H; andwherein the single stranded DNA hybridizes to the single stranded RNA on the substrate and the particle is configured on the substrate such that the particle moves upon mixing RNase H with the DNA hybridized to the RNA.
    • 2. The rolling particle system of claim 1, wherein the DNA is between 5 and 500 nucleotides in length.
    • 3. The rolling particle system of claim 1, wherein the particle has a diameter of 0.001 micrometers to 1 centimeters.
    • 4. The rolling particle system of claim 1, wherein the DNA or the RNA encodes a mutation associated with a genetic disorder.
    • 5. The rolling particle system of claim 1, wherein the DNA has a density of 91,000 molecules/μm2.
      • 6. The rolling particle system of claim 5, wherein RNase H is at a concentration of 140 nM or a 10 fold increase thereof.
  • 7. A rolling rod system comprising, a rod of two or more spherical particles comprising a coating of single stranded DNA;a planar substrate comprising a coating of single stranded RNA, wherein the RNA density is between about 2×104 molecules/μm2 and about 6×104 molecules/μm2, wherein the RNA is conjugated to a fluorescent molecule, wherein the substrate further comprises polyethylene glycol; andRNase H;wherein the single stranded DNA hybridizes to the single stranded RNA on the substrate and the rod is configured on the substrate such that the rod moves upon mixing RNase H with the DNA hybridized to the RNA.
    • 8. The rolling rod system of claim 7, wherein the DNA is between 5 and 500 nucleotides in length.
    • 9. The rolling rod system of claim 7, wherein the DNA or the RNA encodes a mutation associated with a genetic disorder.
    • 10. The rolling particle system of claim 7, wherein the DNA has a density of 91,000 molecules/μm2.
      • 11. The rolling particle system of claim 10, wherein RNase H is at a concentration of 140 nM or a 10 fold increase thereof.